Traditional Chinese medicine composition for treating heart failure and preparation method thereof

Abstract

The invention discloses a traditional Chinese medicine composition for treating heart failure and a preparation method thereof, and relates to the field of traditional Chinese medicines, and the traditional Chinese medicine composition is prepared from red ginseng, coptis chinensis, pericarpium trichosanthis, lumbricus, rhodiola rosea, ganoderma lucidum spore powder, polygonum multiflorum, selfheal, honey-fried licorice root and cassia twig. The preparation method comprises the following steps: soaking the traditional Chinese medicinal materials, decocting, collecting filtrate, filtering, concentrating to obtain extract, and adding ganoderma lucidum spore powder. According to the composition disclosed by the invention, by virtue of the compatibility principle of tonifying deficiency, purging excess and regulating cold and heat, the symptoms of deficiency of yang qi, phlegm-heat stasis resistance, collateral loss and yin exhaustion are accurately matched, and the heart failure complex symptom with preserved ejection fraction of chronic heart failure is remarkably improved.

Description

Technical Field

[0001] The present invention belongs to the field of traditional Chinese medicine, and particularly relates to a traditional Chinese medicine composition for treating heart failure and a preparation method thereof. Background Art

[0002] Chronic heart failure (CHF) is a cardiovascular disease caused by various reasons that damage the myocardium, resulting in reduced ventricular filling ability and weakened ejection function, and presenting symptoms of pulmonary circulation and systemic circulation congestion.

[0003] Currently, the conventional treatment methods mainly rely on drug treatment, including cardiotonic drugs, diuretics, and vasodilators, etc. However, since this disease requires long-term drug treatment, there are often certain adverse reactions in clinical practice. In traditional Chinese medicine theory, CHF belongs to the categories of "heart bi-syndrome", "palpitation", "dyspnea syndrome", "phlegm-fluid retention", etc. It is described in "Huangdi Neijing" as: "If the pulse bi-syndrome persists and is further invaded by pathogenic factors, it will lodge in the heart... For those with heart bi-syndrome, the pulse is blocked, and when troubled, there is throbbing under the heart, sudden shortness of breath and dyspnea, dry throat and frequent belching, and when the reverse qi ascends, there is fear." Thus, it can be seen that the main pathogenesis is due to the decline of heart qi deficiency, which obstructs the meridian circulation, forms blood stasis, and manifests as symptoms of phlegm turbidity and phlegm-fluid retention. The blood stasis and phlegm turbidity obstruct the qi movement, and the flooding of phlegm-fluid consumes yang qi. Repeating in a cycle for a long time will eventually lead to the failure of heart qi. The syndrome differentiation types of chronic heart failure in "Diagnosis and Treatment Guidelines for Common Diseases in Traditional Chinese Internal Medicine" and "Guiding Principles for Clinical Research of New Traditional Chinese Medicines" are specifically: ① syndrome of deficiency of both heart and lung qi; ② syndrome of deficiency of both qi and yin; ③ syndrome of qi deficiency and blood stasis; ④ syndrome of deficiency of both heart and kidney yang; ⑤ syndrome of phlegm-fluid congesting the heart. Traditional Chinese medicine prescriptions mostly target the syndrome types of "qi deficiency and blood stasis" or "syndrome of deficiency of both heart and kidney yang", and there are few research reports on the common composite syndrome types (such as deficiency of yang qi combined with phlegm-heat stasis obstruction and collateral damage with yin exhaustion) of heart failure with preserved ejection fraction (HFpEF).

[0004] CN119074883A, with the invention title "A Traditional Chinese Medicine Composition for Treating Heart Failure with Preserved Ejection Fraction and Its Application", discloses a traditional Chinese medicine composition composed of prepared rehmannia root, deer horn glue, cinnamon, carbonized prepared ginger, white mustard seed, ephedra, processed pinellia ternata, angelica sinensis, and raw licorice root, and points out that this traditional Chinese medicine composition combines warming yang and nourishing blood, and promotes the elimination of phlegm turbidity and dredges blood collaterals simultaneously, enabling the deficiency of yang to be tonified, the nutrient blood to be filled, and the phlegm turbidity and blood stasis to be removed. It can warm the kidney, benefit essence, and assist yang to treat the root cause, and use pungent-warm herbs to resolve phlegm and dredge collaterals to treat the symptoms. However, it only shows that this traditional Chinese medicine composition treats heart failure with preserved ejection fraction of yang deficiency syndrome and phlegm turbidity syndrome, and does not mention the treatment effect on heart failure with phlegm-heat stasis obstruction and collateral damage with yin exhaustion. Summary of the Invention

[0005] The present invention aims at the common composite syndrome types (deficiency of yang qi combined with phlegm-heat stasis obstruction and collateral damage with yin exhaustion) of heart failure with preserved ejection fraction (HFpEF), and provides a traditional Chinese medicine composition for treating heart failure and a preparation method thereof.

[0006] A traditional Chinese medicine composition for treating heart failure according to the present invention, wherein the traditional Chinese medicine composition is composed of 10-20 parts by weight of red ginseng, 5-15 parts by weight of coptis, 10-15 parts by weight of trichosanthes peel, 5-10 parts by weight of earthworm, 10-15 parts by weight of rhodiola rosea, 5-10 parts by weight of ganoderma lucidum spore powder, 5-10 parts by weight of fleeceflower root, 5-10 parts by weight of selfheal spike, 5-10 parts by weight of honey-fried licorice root and 5-10 parts by weight of cassia twig.

[0007] Further, the traditional Chinese medicine composition is composed of 15-20 parts by weight of red ginseng, 10-15 parts by weight of coptis, 12-15 parts by weight of trichosanthes peel, 5-9 parts by weight of earthworm, 12-15 parts by weight of rhodiola rosea, 6-8 parts by weight of ganoderma lucidum spore powder, 5-9 parts by weight of fleeceflower root, 5-9 parts by weight of selfheal spike, 5-6 parts by weight of honey-fried licorice root and 5-6 parts by weight of cassia twig.

[0008] Further, the traditional Chinese medicine composition is composed of 15 parts by weight of red ginseng, 10 parts by weight of coptis, 12 parts by weight of trichosanthes peel, 9 parts by weight of earthworm, 12 parts by weight of rhodiola rosea, 6 parts by weight of ganoderma lucidum spore powder, 9 parts by weight of fleeceflower root, 9 parts by weight of selfheal spike, 6 parts by weight of honey-fried licorice root and 6 parts by weight of cassia twig.

[0009] A preparation method of a traditional Chinese medicine composition for treating heart failure according to the present invention:

[0010] 1) Weigh 10-20 parts by weight of red ginseng, 5-15 parts by weight of coptis, 10-15 parts by weight of trichosanthes peel, 5-10 parts by weight of earthworm, 10-15 parts by weight of rhodiola rosea, 5-10 parts by weight of fleeceflower root, 5-10 parts by weight of selfheal spike, 5-10 parts by weight of honey-fried licorice root and 5-10 parts by weight of cassia twig according to the parts by weight, remove impurities and set aside;

[0011] 2) Add 8-10 times the amount of clear water to the above Chinese medicinal materials, soak them, and then decoct them. After boiling with strong fire, decoct with slow fire for 30-40 minutes, and collect the filtrate; then add 6 times the amount of clear water and decoct for the second time for 20-30 minutes, and collect the filtrate of the second decoction;

[0012] 3) Combine the two filtrates, filter, concentrate to obtain an extract, add 5-10 parts by weight of ganoderma lucidum spore powder, and stir evenly to obtain.

[0013] Further, the relative density of the extract at 20 °C is 1.08-1.12.

[0014] Further, the ganoderma lucidum spore powder is subjected to low-temperature wall-breaking treatment to a particle size of less than 10 μm before being added.

[0015] Further, the earthworm needs to be dried at low temperature, pulverized and passed through an 80-mesh sieve before decocting.

[0016] The present invention targets the main symptoms of yang qi deficiency combined with phlegm-heat stasis obstruction and collateral damage with yin exhaustion: shortness of breath and fatigue, palpitations (qi deficiency and blood stasis), secondary symptoms: chest tightness with copious phlegm, purple tongue with yellow and greasy coating (phlegm-heat stasis obstruction); night sweats and dry mouth (yin deficiency with internal heat); pathogenesis: based on qi deficiency and blood stasis, with phlegm-heat obstructing the lungs and yin deficiency with internal heat as the secondary aspects, which conforms to the pathological characteristics of patients with chronic heart failure (HFpEF) complicated with metabolic syndrome.

[0017] The mechanism of action of the composition of the present invention:

[0018] The sovereign drug: Red ginseng has the efficacy of supplementing qi and promoting blood circulation. It targets the root syndrome of "yang qi deficiency", improves cardiac function by tonifying qi and securing collapse (main symptoms: shortness of breath and fatigue), enhances myocardial contractility by greatly tonifying primordial qi, and improves cardiac output. Ginsenosides in red ginseng promote glucose utilization and enhance myocardial energy metabolism.

[0019] The ministerial drugs: Coptis chinensis (clearing heat and resolving phlegm) + Trichosanthes kirilowii pericarp (relieving chest oppression and regulating qi); Coptis chinensis clears heat and detoxifies (treating "phlegm-heat accumulation inside"), Trichosanthes kirilowii pericarp relieves chest oppression and regulates qi (treating "phlegm obstruction and qi stagnation"), and the combination of the two synergistically improves the secondary syndrome of "phlegm-heat stasis obstruction". Berberine in Coptis chinensis reduces systemic inflammation; Trichosanthes kirilowii pericarp reduces pulmonary artery hypertension.

[0020] The adjuvant drugs: Earthworm, Rhodiola rosea, Polygonum multiflorum, Prunella vulgaris intervene at multiple targets; Earthworm activates blood circulation and dredges collaterals: targeting the pathogenesis of "stasis obstruction", improving blood stasis (purple and dull tongue), and reducing blood viscosity through fibrinolytic enzyme activity. Rhodiola rosea supplements qi and resists hypoxia: enhances the expression of HIF-1α and protects myocardial cells from hypoxic injury. Polygonum multiflorum nourishes the liver and kidney: stilbene glycoside promotes mitochondrial autophagy and improves energy metabolism disorder. Prunella vulgaris clears the liver and resolves phlegm: Prunella vulgaris reduces myocardial fibrosis. Ganoderma lucidum spore powder regulates immunity: relieves systemic inflammation (treating the source of "phlegm-heat").

[0021] The guiding drug: Cinnamon twig warms yang + roasted licorice coordinates; Cinnamon twig is pungent and warm to assist yang (relieving yang deficiency with internal cold), roasted licorice coordinates all the drugs and balances the bitter cold nature of Coptis chinensis, forming a pattern of using cold and heat together, which is suitable for the syndrome of deficiency and excess coexisting.

[0022] The composition of the present invention precisely matches the syndrome type of "yang qi deficiency, phlegm-heat stasis obstruction combined with collateral damage and yin exhaustion" through the compatibility principle of tonifying deficiency and reducing excess, and regulating cold and heat, and significantly improves the complex syndrome type of heart failure with preserved ejection fraction in chronic heart failure.

[0023] The traditional Chinese medicine composition of the present invention improves the syndrome type of "yang qi deficiency combined with phlegm-heat stasis obstruction and collateral damage with yin exhaustion" through the compatibility principle of supplementing qi and warming yang, clearing heat and resolving phlegm, and nourishing yin and dredging collaterals: Energy metabolism repair: Red ginseng + Coptis chinensis work together to improve glucose utilization; Regulation of mitochondrial autophagy: Polygonum multiflorum removes damaged mitochondria; Anti-inflammatory and antioxidant: Coptis chinensis + Ganoderma lucidum spore powder have anti-inflammatory and antioxidant effects. Detailed implementation mode

[0024] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer and more understandable, the spirit of the content disclosed by the present invention will be described in detail below. After any person skilled in the relevant technical field understands the embodiments of the content of the present invention, they can make changes and modifications based on the technology taught by the content of the present invention, which do not depart from the spirit and scope of the content of the present invention.

[0025] The schematic embodiments of the present invention and their descriptions are used to explain the present invention, but do not limit the present invention.

[0026] Example 1

[0027] A method of a traditional Chinese medicine composition for treating heart failure in this embodiment:

[0028] 1) Take 15 g of red ginseng, 10 g of Coptis chinensis, 12 g of Trichosanthes kirilowii Maxim. pericarpium, 9 g of Pheretima aspergillum, 12 g of Rhodiola rosea, 9 g of Polygonum multiflorum Thunb., 9 g of Prunella vulgaris, 6 g of Cinnamomum cassia Presl, and 6 g of roasted licorice root, weigh them according to the proportion, remove impurities and wash them clean; after low-temperature drying, Pheretima aspergillum is pulverized and passed through an 80-mesh sieve;

[0029] 2) Put the above-mentioned medicinal materials into a decocting container, add 8 times the amount of clear water, soak at room temperature for 30 minutes to fully moisten the medicinal materials; for the first decoction: bring to a boil over high heat and then decoct over low heat for 40 minutes, collect the filtrate; for the second decoction: add 6 times the amount of clear water again, decoct for 30 minutes, and collect the filtrate;

[0030] 3) Combine the two filtrates, filter through a 200-mesh filter to remove the medicinal residues, concentrate to obtain an extract (the relative density of the extract at 20 °C is 1.08 - 1.12), and the Ganoderma lucidum spore powder is subjected to low-temperature wall-breaking treatment to a particle size below 10 μm before adding, add it to the extract, stir evenly, divide into portions, seal, and store in the refrigerator.

[0031] Example 2

[0032] Common composite syndromes of heart failure with preserved ejection fraction (HFpEF) in chronic heart failure: yang qi deficiency combined with phlegm-heat stasis obstruction and collateral damage with yin exhaustion. When patients with this syndrome type are combined with metabolic syndrome and pulmonary hypertension, they often show composite symptoms such as shortness of breath and fatigue (qi deficiency), lower limb edema (yang deficiency with fluid retention), chest tightness and excessive phlegm (phlegm-heat obstructing the lung), night sweats and dry mouth (yin deficiency with internal heat).

[0033] The clinical trials in this embodiment are as follows:

[0034] 1. Inclusion criteria

[0035] ① Aged 40 - 75 years old, diagnosed with chronic heart failure (NYHA II - III grade)

[0036] ②The traditional Chinese medicine (TCM) syndrome differentiation conforms to "syndrome of deficiency of yang qi combined with phlegm-heat stasis obstruction and collateral damage with yin exhaustion": The main symptoms are shortness of breath, fatigue, fear of cold with cold limbs, chest tightness with excessive phlegm, purple tongue with yellow and greasy coating, night sweats, and dry mouth (refer to the Guidelines for the Diagnosis and Treatment of Chronic Heart Failure in Traditional Chinese Medicine (2022)), and patients with acute infections, severe liver and kidney diseases, and malignant tumors are excluded.

[0037] 2. Treatment methods

[0038] Control group (n = 15): Qiliqiangxin Capsule (taken according to the instructions).

[0039] Experimental group (n = 15): The decoction prepared in Example 1 (1 dose per day, taken warm in two divided doses in the morning and evening).

[0040] Course of treatment: 12 weeks.

[0041] 3. Observation indicators

[0042] 1) TCM syndrome score: The improvement of main symptoms such as shortness of breath, fear of cold, chest tightness, and night sweats was evaluated according to the Guidelines for Clinical Research of New Chinese Medicines (graded from 0 to 3 points):

[0043] (1) Shortness of breath and fatigue

[0044] 0 point: No shortness of breath after activity, and daily activities are not restricted;

[0045] 1 point: Shortness of breath when walking fast or going upstairs, with mild fatigue;

[0046] 2 points: Shortness of breath when walking slowly on flat ground, with obvious fatigue;

[0047] 3 points: Shortness of breath still felt at rest, with extreme fatigue.

[0048] (2) Fear of cold with cold limbs

[0049] 0 point: Limbs are warm, without fear of cold;

[0050] 1 point: Limbs feel cold after exposure to cold, which can be relieved by wearing clothes;

[0051] 2 points: Persistent fear of cold, requiring thick clothes for warmth;

[0052] 3 points: Cold limbs, accompanied by pale complexion.

[0053] (3) Chest tightness with excessive phlegm

[0054] 0 point: No chest tightness, with little sputum;

[0055] 1 point: Occasionally feel chest tightness, with little sputum;

[0056] 2 points: Frequent chest tightness, with moderate sputum;

[0057] 3 points: Persistent chest tightness, with a large amount of thick and sticky sputum.

[0058] (4) Purple tongue with yellow and greasy fur

[0059] 0 point: Pale red tongue with thin and white fur;

[0060] 1 point: Slightly purple tongue with thin and yellow fur;

[0061] 2 points: Dark purple tongue with yellow and greasy fur;

[0062] 3 points: Dark purple tongue with ecchymosis and yellow and thick greasy fur.

[0063] (5) Night sweats and dry mouth

[0064] 0 point: No night sweats and no dry mouth;

[0065] 1 point: Occasionally have night sweats at night and slightly dry mouth;

[0066] 2 points: Frequent night sweats and need to change clothes;

[0067] 3 points: Sweating profusely, accompanied by severe dry mouth.

[0068] 2) Cardiac function indexes: Left ventricular ejection fraction (LVEF), 6-minute walk distance (6MWD).

[0069] 3) Serum markers: NT-proBNP, IL-6.

[0070] 4) Safety indexes: Liver and kidney functions, electrocardiogram, adverse reaction records.

[0071] 4. Results

[0072] 1) Improvement of traditional Chinese medicine syndromes

[0073] Table 1 Traditional Chinese medicine syndromes

[0074]

[0075] Note: *P<0.05, **P<0.01.

[0076] Before treatment, the symptom distributions of the two groups were balanced. There were no statistical differences in the distributions of age, gender, and underlying diseases (hypertension, diabetes) between the two groups of patients (p>0.05), and they were comparable.

[0077] Improvement rate = (Pre-treatment score - Post-treatment score) / Pre-treatment score × 100%.

[0078] It can be seen from the analysis of the above table that

[0079] (1) Yang qi deficiency (shortness of breath and fatigue, cold limbs)

[0080] The improvement rate of the shortness of breath and fatigue symptom in the experimental group was 65%, and that in the control group was 40%; the improvement rate of the cold limbs symptom in the experimental group was 60%, and that in the control group was 35%;

[0081] (2) Phlegm-heat and blood stasis (chest tightness, excessive phlegm, purple tongue with greasy yellow coating)

[0082] The improvement rate of chest tightness and sputum was 75% in the experimental group and 30% in the control group; the improvement rate of purple tongue coating and greasy yellow tongue coating was 68% in the experimental group and 32% in the control group;

[0083] (3) Damage to the collaterals and exhaustion of Yin (night sweats and dry mouth)

[0084] The improvement rate of night sweats and dry mouth symptoms in the experimental group was 62%, while that in the control group was 25%.

[0085] 2) Cardiac function and serum markers

[0086] Table 2 Cardiac function and serum markers 3) Security

[0087] There were no serious adverse reactions in both groups, and no abnormal changes in liver, kidney function and electrocardiogram.

[0088] Result analysis:

[0089] (1) Yang Qi deficiency (shortness of breath, fatigue, cold body and limbs)

[0090] Red ginseng + cinnamon twig + roasted licorice synergistically warms yang and replenishes qi. Red ginseng: greatly replenishes vital energy and improves myocardial energy metabolism. Red ginseng and cinnamon twig are used together, with red ginseng replenishing qi and strengthening the body as the "lord", and cinnamon twig warming and promoting heart yang as the "minister". The two synergistically enhance cardiac output (LVEF in the experimental group increased from 47.9% to 56.4%, which was more significantly increased than that in the control group to 52.1%), relieve shortness of breath and fatigue, and improve exercise endurance (6MWD in the experimental group increased by 29%, while the control group only increased by 16%). Cinnamon twig: pungent and warm to help yang, enhance microcirculatory blood flow velocity through volatile oil components (such as cinnamaldehyde), and used together with roasted licorice to warm yang and transform qi, offset the bitter and cold nature of coptis chinensis, and form a pattern of regulating cold and heat. Roasted licorice: harmonizes all the medicines, prolongs the half-life of red ginseng and cinnamon twig, and enhances the overall warming yang effect.

[0091] (2) Phlegm-heat and blood stasis (chest tightness, excessive phlegm, purple tongue with greasy yellow coating)

[0092] Coptis chinensis + Trichosanthes peel + Earthworm + Prunella vulgaris synergistically clear heat and resolve phlegm, activate blood circulation and resist fibrosis (NT-proBNP in the experimental group decreased by 66%, while that in the control group decreased by 33%). Coptis chinensis: Berberine inhibits the TLR4 / NF-κB pathway and reduces IL-6 (the decrease in the experimental group was 43%, which was significantly more effective than the 21% decrease in the control group). Coptis chinensis and Panax ginseng together improve energy metabolism. Coptis chinensis is combined with Trichosanthes peel. Coptis chinensis clears heat and detoxifies as the "minister", and Trichosanthes peel soothes the chest and regulates qi as the "assistant", jointly treating phlegm-heat obstructing the lungs. Trichosanthes peel: Improves pulmonary hypertension by reducing the level of ET-1 and relieves chest tightness and excessive phlegm. Trichosanthes peel is combined with Earthworm. Trichosanthes peel resolves phlegm and soothes the chest, while Earthworm dissolves thrombus and dredges collaterals, jointly treating the intermingling of phlegm and stasis. Earthworm: The activity of plasmin improves blood viscosity and relieves purple and dark tongue with ecchymosis. Prunella vulgaris: Ursolic acid inhibits the phosphorylation of TGF-β / Smad3 and reduces the deposition of collagen I / III.

[0093] (3) Collateral damage with yin exhaustion (night sweats and dry mouth)

[0094] Rhodiola rosea + Ganoderma lucidum spore powder + Polygonum multiflorum synergistically nourish yin and dredge collaterals, and have antioxidant effects. Polygonum multiflorum: Stilbene glycoside activates the SIRT1 / PINK1 pathway, promotes mitochondrial autophagy, and clears damaged mitochondria. Polygonum multiflorum is combined with Rhodiola rosea. Polygonum multiflorum nourishes the yin of the liver and kidneys, and Rhodiola rosea resists hypoxia, jointly treating collateral damage with yin exhaustion. Rhodiola rosea: Protects myocardial cells from hypoxic injury and relieves night sweats. Ganoderma lucidum spore powder: Has antioxidant effects and scavenges ROS.

[0095] It can be seen from this that the traditional Chinese medicine composition of the present invention has a significant therapeutic effect on the common composite syndromes of heart failure with preserved ejection fraction (HFpEF) in chronic heart failure: yang qi deficiency combined with phlegm-heat stasis obstruction and collateral damage with yin exhaustion.

[0096] Example 3

[0097] Mouse experiment

[0098] 1) Model: 8 - 10-week-old male C57BL / 6J mice were used. Starting from the 3rd day after TAC surgery, they were fed a high-fat diet (containing 45% fat, 20% protein, and 35% carbohydrates) for 8 - 12 weeks.

[0099] 2) Grouping: Control group (sham operation + normal diet + normal saline); Model group (normal saline); Experimental group: low dose (3 g / kg), medium dose (6 g / kg), high dose (9 g / kg) of the traditional Chinese medicine composition in Example 1; Positive control group: Qiliqiangxin Capsule (1.2 g / kg).

[0100] 3) Detection indicators: Blood glucose clearance rate: Glucose (2 g / kg) was intraperitoneally injected, and blood glucose was measured by tail vein blood sampling (0, 15, 30, 60 min). The number of myocardial mitochondrial autophagosomes was observed by transmission electron microscopy. The LC3-II / I ratio was detected. The serum TNF-α level and IL-6 were detected by ELISA, and the expression level of myocardial SOD2 was detected.

[0101] 4) Results

[0102] Table 3 Energy metabolism repair

[0103]

[0104] The results in the above table show that the blood glucose clearance rate of the experimental group (medium dose) was increased to 72%, indicating that the traditional Chinese medicine composition has the function of energy metabolism repair.

[0105] Table 4 Mitochondrial autophagy

[0106]

[0107] Note: **P < 0.01.

[0108] The results in the above table show that Polygonum multiflorum in the traditional Chinese medicine composition promotes mitochondrial autophagy, and the LC3-II / I ratio of the medium-dose experimental group was significantly increased to 1.1 compared with 0.3 in the model group. The ability to clear damaged mitochondria was significant (the number of autophagosomes in the medium-dose experimental group was increased by 4 times compared with the model group)

[0109] Table 5 Anti-inflammatory and antioxidant

[0110]

[0111] Note: **P < 0.01.

[0112] The results in the above table show that the combination of Coptis chinensis and Ganoderma lucidum spores in the traditional Chinese medicine composition synergistically improved the anti-inflammatory and antioxidant abilities. The TNF-α in the medium-dose group was reduced by 46% compared with the model group, and IL-6 was reduced by 40% compared with the model group. The expression level of SOD2 was increased by about 37%.

[0113] In summary, the traditional Chinese medicine composition of the present invention improves energy metabolism, mitochondrial autophagy, and inflammatory oxidative stress through multi-target synergistic effects, and its curative effect is significantly better than that of the positive control group (Qiliqiangxin Capsule).

Claims

1. A traditional Chinese medicine composition for treating heart failure, characterized in that The traditional Chinese medicine composition is composed of 10-20 parts by weight of red ginseng, 5-15 parts by weight of Coptis chinensis, 10-15 parts by weight of Trichosanthes kirilowii Maxim. pericarpium, 5-10 parts by weight of Pheretima, 10-15 parts by weight of Rhodiola rosea, 5-10 parts by weight of Ganoderma lucidum spores powder, 5-10 parts by weight of Polygonum multiflorum Thunb., 5-10 parts by weight of Prunella vulgaris, 5-10 parts by weight of honey-fried licorice root and 5-10 parts by weight of Ramulus Cinnamomi.

2. The traditional Chinese medicine composition for treating heart failure according to claim 1, wherein The traditional Chinese medicine composition is composed of 15-20 parts by weight of red ginseng, 10-15 parts by weight of Coptis chinensis, 12-15 parts by weight of Trichosanthes kirilowii Maxim. pericarpium, 5-9 parts by weight of Pheretima, 12-15 parts by weight of Rhodiola rosea, 6-8 parts by weight of Ganoderma lucidum spores powder, 5-9 parts by weight of Polygonum multiflorum Thunb., 5-9 parts by weight of Prunella vulgaris, 5-6 parts by weight of honey-fried licorice root and 5-6 parts by weight of Ramulus Cinnamomi.

3. To prepare a traditional Chinese medicine composition for treating heart failure as described in claim 1, characterized in that The traditional Chinese medicine composition is composed of 15 parts by weight of red ginseng, 10 parts by weight of Coptis chinensis, 12 parts by weight of Trichosanthes kirilowii Maxim. pericarpium, 9 parts by weight of Pheretima, 12 parts by weight of Rhodiola rosea, 6 parts by weight of Ganoderma lucidum spores powder, 9 parts by weight of Polygonum multiflorum Thunb., 9 parts by weight of Prunella vulgaris, 6 parts by weight of honey-fried licorice root and 6 parts by weight of Ramulus Cinnamomi.

4. A method for preparing a traditional Chinese medicine composition for treating heart failure according to claim 1, characterized in that The preparation method of the traditional Chinese medicine composition is as follows: 1) Weigh 10-20 parts by weight of red ginseng, 5-15 parts by weight of Coptis chinensis, 10-15 parts by weight of Trichosanthes kirilowii Maxim. pericarpium, 5-10 parts by weight of Pheretima, 10-15 parts by weight of Rhodiola rosea, 5-10 parts by weight of Polygonum multiflorum Thunb., 5-10 parts by weight of Prunella vulgaris, 5-10 parts by weight of honey-fried licorice root and 5-10 parts by weight of Ramulus Cinnamomi according to the parts by weight, remove impurities and reserve for use; 2) Add 8-10 times the amount of clear water to the above Chinese medicinal materials, soak them, and then decoct. After boiling with strong fire, decoct with slow fire for 30-40 min, and collect the filtrate; then add 6 times the amount of clear water and decoct for the second time for 20-30 min, and collect the filtrate of the second decoction; 3) Combine the two filtrates, filter, concentrate to obtain an extract, add 5-10 parts by weight of Ganoderma lucidum spores powder, and stir evenly to obtain.

5. The preparation method of a traditional Chinese medicine composition for treating heart failure according to claim 4, wherein The relative density of the extract at 20 °C is 1.08-1.

12.

6. The preparation method of a traditional Chinese medicine composition for treating heart failure according to claim 4, wherein Before adding the Ganoderma lucidum spores powder, it is subjected to low-temperature wall-breaking treatment until the particle size is below 10 μm.

7. The preparation method of a traditional Chinese medicine composition for treating heart failure according to claim 4, characterized in that The Pheretima needs to be dried at low temperature, crushed through an 80-mesh sieve, and then decocted.

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