Ethanol-free pandanus odoratus tablet with anti-depression effect and preparation method of ethanol-free pandanus odoratus tablet

Abstract

The invention discloses an ethanol-free pandan scented tablet with an anti-depression effect. The ethanol-free pandan scented tablet is prepared from the following raw materials in parts by weight: 30-50 parts of pandan, 10-20 parts of rose, 5-15 parts of ambrota and 5-10 parts of amber. Through the synergistic effect of the pandan and other natural perfumes, the anti-depression function of the perfumed tablet is achieved. Researches show that the pandan leaves are rich in active ingredients such as 2-acetyl-1-pyrroline (2-AP), beta-caryophyllene and the like, and can stimulate a brain marginal system through an olfactory pathway, promote release of 5-hydroxytryptamine (5-HT) and dopamine (DA) and remarkably improve the emotional state. In addition, linalool of rose and ambrota ether of ambrota in the formula form a synergistic effect, a prefrontal cortex 5-HT1A receptor is activated, the alpha brain wave power spectrum density is enhanced, and rapid emotion regulation is achieved. According to the ethanol-free pandan scented tablet formula, side effects of traditional antidepressant drugs are avoided, a safe and convenient emotion intervention scheme is provided through natural aromatherapy, and the ethanol-free pandan scented tablet formula conforms to the non-drug mental health management trend advocated by WHO.

Description

Technical Field

[0001] The present invention relates to the technical field of ethanol - free pandan scented tablets, and particularly to an ethanol - free pandan scented tablet with antidepressant efficacy and a preparation method thereof. Background Art

[0002] Scented tablets are a type of fragrance product that can be used to improve the air quality in cars and add a comfortable atmosphere, slowly releasing fragrance through air circulation.

[0003] (1) Traditional scented tablets mostly rely on ethanol as a solvent, which is likely to cause skin irritation and pungent odors. Moreover, pandan scented tablets containing ethanol are highly flammable items and are prohibited from being carried on cars, railways, and airplanes. Encountering an open flame will trigger phenomena such as fire and explosion.

[0004] (2) In modern society, with greater pressure, the number of people suffering from depression is increasing. Existing scented tablets do not have the function of antidepressant. Summary of the Invention

[0005] The purpose of the present invention is to provide an ethanol - free pandan scented tablet with antidepressant efficacy and a preparation method thereof to solve the problems raised in the above background art.

[0006] To achieve the above purpose, the present invention provides the following technical solutions:

[0007] An ethanol - free pandan scented tablet with antidepressant efficacy, comprising raw materials composed of the following parts by weight: 30 - 50 parts of pandan, 10 - 20 parts of rose, 5 - 15 parts of ambergris, and 5 - 10 parts of amber.

[0008] As a further scheme of the present invention: it comprises raw materials composed of the following parts by weight: 40 - 50 parts of pandan, 10 - 18 parts of rose, 7 - 15 parts of ambergris, and 6 - 10 parts of amber.

[0009] As a further scheme of the present invention: it comprises raw materials composed of the following parts by weight: 45 parts of pandan, 15 parts of rose, 10 parts of ambergris, and 8 parts of amber.

[0010] A preparation method of an ethanol - free pandan scented tablet with antidepressant efficacy, comprising the following steps:

[0011] S1: Select fresh pandan leaves according to parts by weight, and obtain a green oily substance through supercritical carbon dioxide extraction;

[0012] S2: Weigh rose, ambergris, and amber according to parts by weight and crush them into powders with a particle size of 100 mesh;

[0013] S3: Mix the green oily substance obtained in S1 with the powder obtained in S2 and stir for homogenization;

[0014] S4: Inject into the mold, cure at room temperature for 24 hours, cut into 5 cm × 5 cm incense tablets, and package them in an aluminum foil bag for storage away from light.

[0015] As a further scheme of the present invention: In S1, the critical temperature of carbon dioxide for supercritical carbon dioxide extraction is 45 °C, the critical pressure is 25 MPa, and the extraction duration is 2 hours.

[0016] As a further scheme of the present invention: In S3, the stirring and homogenization are carried out at 25 - 30 °C, the stirring speed is 200 - 300 rpm, and the duration is 30 - 60 minutes.

[0017] As a further scheme of the present invention: In S4, the room-temperature curing is carried out under the condition of a relative humidity of 40% - 60%.

[0018] As a still further scheme of the present invention: In S4, the thickness of the incense tablet is 2 - 5 mm.

[0019] Compared with the prior art, the beneficial effects of the present invention are:

[0020] Through the synergistic effect of pandan and other natural fragrances, the present invention realizes the antidepressant function of the incense tablet. Research shows that the pandan leaves are rich in active ingredients such as 2-acetyl-1-pyrroline (2-AP) and β-caryophyllene, which can stimulate the limbic system of the brain through the olfactory pathway, promote the release of 5-hydroxytryptamine (5-HT) and dopamine (DA), and significantly improve the mood state. In addition, the linalool of rose in the formula and the ambrein of ambergris form a synergistic effect, activate the 5-HT1A receptor in the prefrontal cortex, and enhance the power spectral density of α brain waves to achieve rapid mood regulation. The ethanol-free pandan incense tablet formula of the present invention avoids the side effects of traditional antidepressant drugs (such as drowsiness and dry mouth), provides a safe and convenient mood intervention plan through natural aromatherapy, and conforms to the trend of non-drug mental health management advocated by the WHO. Description of the Drawings

[0021] Figure 1 It is a process flow chart of the preparation method of the ethanol-free pandan incense tablet with antidepressant efficacy. Detailed Embodiments

[0022] Hereinafter, various exemplary embodiments, features, and aspects of the present application will be described in detail with reference to the drawings. The same reference numerals in the drawings denote elements having the same or similar functions. Although various aspects of the embodiments are shown in the drawings, the drawings do not have to be drawn to scale unless otherwise specified.

[0023] The special term "exemplary" here means "serving as an example, embodiment, or illustrative". Any embodiment described as "exemplary" here does not have to be construed as superior to or better than other embodiments.

[0024] In addition, for a better illustration of the present application, numerous specific details are given in the following specific embodiments. Those skilled in the art should understand that the present application can be implemented without certain specific details. In some instances, methods, means, and components well-known to those skilled in the art are not described in detail in order to highlight the gist of the present application.

[0025] Example 1

[0026] An ethanol-free pandan scented tablet with antidepressant efficacy, comprising raw materials composed of the following parts by weight: 30 parts of pandan, 10 parts of rose, 5 parts of ambergris, and 5 parts of amber.

[0027] A method for preparing an ethanol-free pandan scented tablet with antidepressant efficacy, comprising the following steps:

[0028] S1: Select fresh pandan leaves according to parts by weight, and obtain a green oily substance through supercritical carbon dioxide extraction;

[0029] S2: Weigh rose, ambergris, and amber according to parts by weight, and crush them into powders with a particle size of 100 mesh;

[0030] S3: Mix the green oily substance obtained in S1 with the powder obtained in S2, and stir and homogenize;

[0031] S4: Inject into a mold, cure at room temperature for 24 hours, cut into 5 cm × 5 cm scented tablets, and package them in an aluminum foil bag for storage in the dark.

[0032] Preferably, in this embodiment, in S1, the critical temperature of carbon dioxide for supercritical carbon dioxide extraction is 45 °C, the critical pressure is 25 MPa, and the extraction duration is 2 hours.

[0033] Preferably, in this embodiment, in S3, the stirring and homogenization are carried out at 25 - 30 °C, the stirring speed is 200 - 300 rpm, and the duration is 30 - 60 minutes.

[0034] Preferably, in this embodiment, in S4, the room temperature curing is carried out under the condition of a relative humidity of 40% - 60%.

[0035] Preferably, in this embodiment, in S4, the thickness of the scented tablet is 2 - 5 mm.

[0036] Example 2

[0037] An ethanol-free pandan scented tablet with antidepressant efficacy, comprising raw materials composed of the following parts by weight: 50 parts of pandan, 20 parts of rose, 15 parts of ambergris, and 10 parts of amber.

[0038] A preparation method of ethanol-free pandan scented tablets with antidepressant efficacy, comprising the following steps:

[0039] S1: Select fresh pandan leaves by weight, and obtain a green oily substance through supercritical carbon dioxide extraction;

[0040] S2: Weigh rose, ambergris, and amber by weight, and crush them into a powder with a particle size of 100 mesh;

[0041] S3: Mix the green oily substance obtained in S1 with the powder obtained in S2, and stir and homogenize;

[0042] S4: Inject into a mold, cure at room temperature for 24 hours, cut into 5 cm × 5 cm scented tablets, and package them in an aluminum foil bag for storage in the dark.

[0043] Preferably, in this embodiment, in S1, the critical temperature of carbon dioxide for supercritical carbon dioxide extraction is 45 °C, the critical pressure is 25 MPa, and the extraction duration is 2 hours.

[0044] Preferably, in this embodiment, in S3, the stirring and homogenization are carried out at 25-30 °C, the stirring speed is 200-300 rpm, and the duration is 30-60 minutes.

[0045] Preferably, in this embodiment, in S4, the room temperature curing is carried out under the condition of a relative humidity of 40%-60%.

[0046] Preferably, in this embodiment, in S4, the thickness of the scented tablet is 2-5 mm.

[0047] Example Three

[0048] An ethanol-free pandan scented tablet with antidepressant efficacy, comprising the following raw materials by weight: 40 parts of pandan, 10 parts of rose, 7 parts of ambergris, and 6 parts of amber.

[0049] A preparation method of ethanol-free pandan scented tablets with antidepressant efficacy, comprising the following steps:

[0050] S1: Select fresh pandan leaves by weight, and obtain a green oily substance through supercritical carbon dioxide extraction;

[0051] S2: Weigh rose, ambergris, and amber by weight, and crush them into a powder with a particle size of 100 mesh;

[0052] S3: Mix the green oily substance obtained in S1 with the powder obtained in S2, and stir and homogenize;

[0053] S4: Inject into a mold, cure at room temperature for 24 hours, cut into 5 cm × 5 cm scented tablets, and package them in an aluminum foil bag for storage in the dark.

[0054] Preferably, in this embodiment, in S1, the critical temperature of carbon dioxide for supercritical carbon dioxide extraction is 45 °C, the critical pressure is 25 MPa, and the extraction duration is 2 hours.

[0055] Preferably, in this embodiment, in S3, the stirring and homogenization are carried out at 25 - 30 °C, the stirring speed is 200 - 300 rpm, and the duration is 30 - 60 minutes.

[0056] Preferably, in this embodiment, in S4, the room-temperature curing is carried out under the condition of a relative humidity of 40% - 60%.

[0057] Preferably, in this embodiment, in S4, the thickness of the scented sachet is 2 - 5 mm.

[0058] Example 4

[0059] An ethanol-free pandan scented sachet with antidepressant efficacy, comprising the following raw materials by weight: 50 parts of pandan, 18 parts of rose, 15 parts of ambergris, and 10 parts of amber.

[0060] A preparation method of an ethanol-free pandan scented sachet with antidepressant efficacy, comprising the following steps:

[0061] S1: Select fresh pandan leaves by weight, and obtain a green oily substance through supercritical carbon dioxide extraction;

[0062] S2: Weigh rose, ambergris, and amber by weight, and crush them into powders with a particle size of 100 mesh;

[0063] S3: Mix the green oily substance obtained in S1 with the powder obtained in S2, and stir and homogenize;

[0064] S4: Inject into a mold, cure at room temperature for 24 hours, cut into 5 cm × 5 cm scented sachets, and package them in an aluminum foil bag for light-proof storage.

[0065] Preferably, in this embodiment, in S1, the critical temperature of carbon dioxide for supercritical carbon dioxide extraction is 45 °C, the critical pressure is 25 MPa, and the extraction duration is 2 hours.

[0066] Preferably, in this embodiment, in S3, the stirring and homogenization are carried out at 25 - 30 °C, the stirring speed is 200 - 300 rpm, and the duration is 30 - 60 minutes.

[0067] Preferably, in this embodiment, in S4, the room-temperature curing is carried out under the condition of a relative humidity of 40% - 60%.

[0068] Preferably, in this embodiment, in S4, the thickness of the scented chip is 2 - 5 mm.

[0069] Example Five

[0070] An ethanol - free pandan scented chip with antidepressant efficacy, comprising raw materials composed of the following parts by weight: 45 parts of pandan, 15 parts of rose, 10 parts of ambergris, and 8 parts of amber.

[0071] A preparation method of an ethanol - free pandan scented chip with antidepressant efficacy, comprising the following steps:

[0072] S1: Select fresh pandan leaves by weight, and obtain a green oily substance through supercritical carbon dioxide extraction;

[0073] S2: Weigh rose, ambergris, and amber by weight, and crush them into powders with a particle size of 100 mesh;

[0074] S3: Mix the green oily substance obtained in S1 with the powder obtained in S2, and stir for homogenization;

[0075] S4: Inject into a mold, cure at room temperature for 24 hours, cut into 5 cm × 5 cm scented chips, and encapsulate them in an aluminum foil bag for storage in the dark.

[0076] Preferably, in this embodiment, in S1, the critical temperature of carbon dioxide for supercritical carbon dioxide extraction is 45 °C, the critical pressure is 25 MPa, and the extraction duration is 2 hours.

[0077] Preferably, in this embodiment, in S3, the stirring for homogenization is carried out at 25 - 30 °C, the stirring speed is 200 - 300 rpm, and the duration is 30 - 60 minutes.

[0078] Preferably, in this embodiment, in S4, the room - temperature curing is carried out under the condition of a relative humidity of 40% - 60%.

[0079] Preferably, in this embodiment, in S4, the thickness of the scented chip is 2 - 5 mm.

[0080] Experiment 1: Verification of the release of active ingredients

[0081] Use gas chromatography - mass spectrometry to monitor the characteristics of the scented chip at 25 °C;

[0082]

[0083]

[0084] Experiment 2: Animal behavior test (mouse forced swimming test)

[0085] **p < 0.01 vs. control group; Exposure condition: Enclosed space (1 m 3 ) hanging scented chips for 2 hours per day for 14 consecutive days;

[0086] Group Immobile time (s) 5-HT level (ng / mL) DA level (pg / mL) Control group 188±21 32.4±3.1 415±38 Incense chip exposure group 102±15** 58.7±4.5** 692±52** Fluoxetine group 89±12 62.1±5.2 705±49

[0087] Data indicate that the antidepressant effect of the present invention is comparable to that of fluoxetine, and there are no side effects such as weight loss or activity inhibition.

[0088] Experiment 3: Neuroreceptor specificity (in vitro CHO cell model)

[0089] Calcium ion fluorescence labeling method, concentration gradient of scented chip extract: 0.1 - 10 μg / mL;

[0090] Receptor type Activation rate (vs control group) EC50 (μg / mL) 5-HT1A 182％±15％** 0.32 5-HT2A 105％±8％ ＞10 D2 123％±9％* 1.78

[0091] Data show that the present invention can activate 5-HT1A receptors in the prefrontal cortex, enhance the power spectral density of α brain waves, and achieve rapid mood regulation.

[0092] Experiment 4: Human trial

[0093] Subjects: 60 patients with mild to moderate depression (BDI-II score ≥ 14), divided into two groups.

[0094] Intervention: The experimental group used scented chips for 2 hours per day, and the control group used inactive scented chips.

[0095] Results (after 4 weeks):

[0096]

[0097]

[0098] *p < 0.05, **p < 0.01. Experimental data indicate that the present invention is safe and effective.

[0099] Through the synergistic effect of pandan and other natural fragrances, the present invention realizes the antidepressant function of scented chips. Research shows that pandan leaves are rich in active ingredients such as 2-acetyl-1-pyrroline (2-AP) and β-caryophyllene, which can stimulate the limbic system of the brain through the olfactory pathway, promote the release of serotonin (5-HT) and dopamine (DA), and significantly improve the emotional state. In addition, the linalool of rose and the ambrein of ambergris in the formula form a synergistic effect, activate 5-HT1A receptors in the prefrontal cortex, enhance the power spectral density of α brain waves, and achieve rapid mood regulation. The ethanol-free pandan scented chip formula of the present invention avoids the side effects of traditional antidepressant drugs (such as drowsiness and dry mouth), provides a safe and convenient mood intervention program through natural aromatherapy, and conforms to the trend of non-drug mental health management advocated by the WHO.

[0100] For those skilled in the art, it is obvious that the present invention is not limited to the details of the above-mentioned exemplary embodiments, and the present invention can be implemented in other specific forms without departing from the spirit or basic characteristics of the present invention. Therefore, from any point of view, the embodiments should be regarded as exemplary and non-limiting. The scope of the present invention is defined by the appended claims rather than the above description. Therefore, all changes falling within the meaning and scope of the equivalent elements of the claims are intended to be embraced within the present invention.

[0101] In addition, it should be understood that although this specification is described according to embodiments, not every embodiment only contains an independent technical solution. This narrative manner of the specification is only for clarity. Those skilled in the art should regard the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A non-ethanol pandan scented tablet with antidepressant effect, characterized in that, The raw materials include the following components by weight: 30 - 50 parts of pandan, 10 - 20 parts of rose, 5 - 15 parts of ambergris, and 5 - 10 parts of amber.

2. The ethanol-free pandan scented tablets with antidepressant efficacy according to claim 1, characterized in that, The raw materials include the following components by weight: 40 - 50 parts of pandan, 10 - 18 parts of rose, 7 - 15 parts of ambergris, and 6 - 10 parts of amber.

3. The ethanol-free pandan scented tablets with antidepressant efficacy according to claim 1, characterized in that, The raw materials include the following components by weight: 45 parts of pandan, 15 parts of rose, 10 parts of ambergris, and 8 parts of amber.

4. A preparation method of an ethanol-free pandan scented tablet with antidepressant efficacy as described in claims 1-3, characterized in that, It includes the following steps: S1: Select fresh pandan leaves by weight, and obtain a green oily substance through supercritical carbon dioxide extraction. S2: Weigh rose, ambergris, and amber by weight, and crush them into powders with a particle size of 100 mesh. S3: Mix the green oily substance obtained in S1 with the powder obtained in S2, and stir and homogenize. S4: Inject into a mold, cure at room temperature for 24 hours, cut into 5 cm × 5 cm incense tablets, and encapsulate them in an aluminum foil bag for storage away from light.

5. The preparation method of the ethanol-free pandan scented tablets with antidepressant efficacy according to claim 4, characterized in that, In S1, the critical temperature of carbon dioxide for supercritical carbon dioxide extraction is 45 °C, the critical pressure is 25 MPa, and the extraction duration is 2 hours.

6. The preparation method of the ethanol-free pandan scented tablets with antidepressant efficacy according to claim 4, characterized in that, In S3, the stirring and homogenization are carried out at 25 - 30 °C, the stirring speed is 200 - 300 rpm, and the duration is 30 - 60 minutes.

7. The preparation method of the ethanol-free pandan scented tablets with antidepressant efficacy according to claim 4, characterized in that In S4, the room temperature curing is carried out under the condition of a relative humidity of 40% - 60%.

8. The preparation method of the ethanol-free pandan scented tablets with antidepressant efficacy according to claim 4, characterized in that, In S4, the thickness of the incense tablet is 2 - 5 mm.

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