A ganoderma lucidum peptide composition, a preparation method therefor, and an application thereof

Abstract

The present application relates to ganoderma peptide technical field, especially to a kind of ganoderma peptide composition and its preparation method and application.The composition includes the following component by mass fraction:ganoderma peptide 30-50 parts, purple cone chrysanthemum polysaccharide 10-20 parts, polygonatum polysaccharide 8-18 parts, dendrobium polysaccharide 5-25 parts, astragalus polysaccharide 9-16 parts, acanthopanax polysaccharide 12-24 parts, 20-50 parts of probiotic active bacteria powder, the number of active bacteria in the probiotic active bacteria powder is 4x10 10 CFU / g-6x10 20 CFU / g.The ganoderma peptide composition provided by the present application has excellent immunoregulatory function, can improve body immunity, enhance physical quality, resist pathogen invasion.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of ganoderma lucidum peptides, and particularly relates to a ganoderma lucidum peptide composition and a preparation method and application thereof. BACKGROUND

[0002] Ganoderma lucidum peptide is a kind of bioactive peptide extracted, separated from ganoderma or obtained by means of biological technology enzymolysis. It is connected by multiple amino acids with a peptide bond, and has a relatively small molecular weight. This characteristic enables it to be more efficiently absorbed by the gastrointestinal tract of the human body, avoiding the problem of incomplete digestion and absorption of some macromolecular proteins, and enabling it to quickly enter the blood circulation and exert efficacy. Ganoderma lucidum peptide contains a variety of essential amino acids for the human body. The reasonable collocation of these amino acids is the material basis for its numerous physiological activities. For example, branched-chain amino acids such as leucine and isoleucine in it are of great significance for muscle repair and energy metabolism regulation. At present, as an immunomodulator, ganoderma lucidum peptide can activate immune cells such as macrophages, NK cells, T cells and B cells in the human immune system, promote them to release cytokines, enhance the body's resistance to external pathogens, and help prevent colds, influenza and other infectious diseases, so it is often made into health products for people with low immunity to take regularly. However, the effect of simply taking ganoderma lucidum peptide is limited, and the function of improving immunity is limited.

[0003] CN110522901A discloses a composition of lamb bone marrow peptide and ganoderma lucidum peptide, and the raw materials include, by weight ratio, 1020 parts of lamb bone marrow peptide, 1020 parts of ganoderma lucidum peptide polysaccharide, 0.5-1.6 parts of SGF hydrolytic enzyme, 510 parts of animal protein extract, 510 parts of natural vegetable extract, 510 parts of seaweed extract, 35 parts of milk, 510 parts of wheat extract, 13 parts of thickening agent, 13 parts of sweetener and the balance of pure water. The application enhances the hematopoietic function of human bone marrow and improves immunity through the synergistic effect of lamb bone marrow peptide and ganoderma lucidum peptide. However, the two kinds of peptide components are superimposed, and when they are digested and absorbed in the gastrointestinal tract, the body's metabolic burden will be increased. Especially for the elderly and patients with gastrointestinal diseases who have poor digestive function, such as slow gastrointestinal peristalsis and insufficient secretion of digestive enzymes, a large amount of peptide substances suddenly enter the digestive system, which is more difficult to be fully decomposed and absorbed, and is easy to cause symptoms such as abdominal distension, belching and indigestion.

[0004] CN104522461A discloses a ganoderma peptide and rhizoma polygonati polysaccharide and a preparation process thereof, which is composed of ganoderma peptide polysaccharide, rhizoma polygonati polysaccharide and urtica polysaccharide, and the weight percentage is: ganoderma peptide polysaccharide 20-60%, rhizoma polygonati polysaccharide 10-50% and urtica polysaccharide 10-50%. The health-care food is prepared by culturing, filtering, purifying, drying, crushing, compounding and filling. The health-care food provided by the application has obvious effects of detoxification, anti-inflammation and analgesia, and also has the effects of dispelling wind, dredging collaterals and regulating nerve center. However, the composition of the application does not have the effect of improving immunity.

[0005] In view of this, the present application is proposed. SUMMARY

[0006] The present application aims to provide a ganoderma peptide composition and a preparation method and application thereof. The ganoderma peptide composition provided by the present application has excellent immune regulation function, and can improve immunity and enhance physical quality and resist pathogen invasion.

[0007] In order to achieve the above-mentioned purpose, the present application provides the following technical solutions.

[0008] In a first aspect, the present application provides a ganoderma peptide composition, which comprises the following components by mass fraction: ganoderma peptide 30-50 parts, echinacea polysaccharide 10-20 parts, rhizoma polygonati polysaccharide 8-18 parts, dendrobium polysaccharide 5-25 parts, astragalus polysaccharide 9-16 parts, acanthopanax polysaccharide 12-24 parts and 20-50 parts of probiotic active bacteria powder. The number of active bacteria in the probiotic active bacteria powder is 4 x 10 10 CFU / g-6 x 10 20 CFU / g.

[0009] In a preferred embodiment, the composition comprises the following components by mass fraction: ganoderma peptide 33-46 parts, echinacea polysaccharide 12-18 parts, rhizoma polygonati polysaccharide 10-16 parts, dendrobium polysaccharide 10-18 parts, astragalus polysaccharide 11-14 parts, acanthopanax polysaccharide 15-22 parts and probiotic active bacteria powder 25-40 parts.

[0010] The present application can improve immunity from the aspects of immune cell activation, immune factor regulation, antioxidant and anti-stress and intestinal flora optimization by compounding ganoderma peptide and traditional Chinese medicine extract polysaccharide.

[0011] At the level of immune cell activation, echinacea polysaccharides can activate macrophages and natural killer cells, enhance their ability to phagocytize and kill pathogens; astragalus polysaccharides can promote lymphocyte proliferation and differentiation, and enhance T cell and B cell activity. When they work together, different polysaccharides from their respective immune cell types cut in, stimulating more types of immune cells to be awakened and activated, and ganoderma peptides can also help regulate immune cell signaling, making the activation process smoother and more efficient, and improving immunity in all directions. At the same time, eleutherococcus polysaccharides help strengthen the memory function of immune cells, and when the same pathogen invades again, the immune response can be quickly and powerfully initiated. In combination with dendrobium polysaccharides, dendrobium polysaccharides provide energy for immune cell metabolism and proliferation, stabilize the intracellular environment, and allow immune memory cells to survive longer and function more strongly, while ganoderma peptides assist in modifying the recognition tags of immune memory cells, further consolidating the body's memory of pathogens.

[0012] At the level of immune factor regulation, polygonatum polysaccharides induce the body to produce interferons, interleukins and other immune factors, and astragalus polysaccharides also stimulate the release of various immune regulatory factors. The combination of multiple polysaccharides significantly increases the concentration of immune factors in the body within a short period of time, and ganoderma peptides act as a regulatory switch to precisely control the appropriate secretion amount and action duration of various immune factors, preventing excessive immune response and building a balanced and active immune regulation network. At the same time, these polysaccharides and ganoderma peptides together create a stable internal microenvironment, avoiding the inactivation of immune factors due to changes in temperature, pH, or enzymatic degradation, and ensuring the sustained pressure of immune factors on pathogens for a long time.

[0013] At the level of antioxidant and stress resistance, echinacea polysaccharides, polygonatum polysaccharides, and other antioxidants can efficiently scavenge free radicals generated by the body's metabolism and pathogen invasion, reducing the damage to immune cells and immune factors caused by free radicals, and ganoderma peptides can repair cell membranes and organelles damaged by free radicals, restoring the vitality and function of immune cells. At the same time, in the face of external stress, such as pathogen infection, eleutherococcus polysaccharides and astragalus polysaccharides regulate the secretion of stress hormones in the body, weakening the inhibition of the immune system by stress, and ganoderma peptides enhance the tolerance of immune cells to stress, allowing the immune system to maintain high combat effectiveness in adverse environments.

[0014] At the level of intestinal flora optimization, polysaccharides such as dendrobium polysaccharides and polygonatum polysaccharides are food for beneficial bacteria in the gut, promoting the proliferation of beneficial bacteria such as bifidobacteria and lactobacilli, and eleutherococcus polysaccharides regulate the distribution of intestinal flora, creating a healthy intestinal microecology. At the same time, the fermentation of polysaccharides by beneficial bacteria produces metabolites such as short-chain fatty acids, some of which can be absorbed indirectly to stimulate the immune system, and ganoderma peptides assist in repairing the intestinal barrier to prevent pathogens from invading from the gut, strengthening the immune defense line from the gut.

[0015] Further, the present application simultaneously adds probiotic live powder, which cooperates with various traditional Chinese medicine extracts to improve the body's immunity, and simultaneously solves the problem that traditional Chinese medicine extracts are difficult to be fully decomposed and absorbed.

[0016] In a preferred embodiment, the probiotic live powder is a mixture of Bifidobacterium live powder and Lactobacillus rhamnosus live powder, and the mass ratio of the Bifidobacterium live powder and the Lactobacillus rhamnosus live powder is 1:1.

[0017] In a preferred embodiment, the number of live bacteria in the Bifidobacterium live powder is 2 x 10 10 CFU / g-3 x 10 20 CFU / g.

[0018] In a preferred embodiment, the number of live bacteria in the Lactobacillus rhamnosus live powder is 2 x 10 10 CFU / g-3 x 10 20 CFU / g.

[0019] The present application further limits the probiotics to Bifidobacterium and Lactobacillus rhamnosus, which synergistically improve the function of the composition in regulating immunity in terms of intestinal barrier reinforcement, immune cell activation, immune factor regulation, and metabolic product synergism.

[0020] In terms of intestinal barrier reinforcement, Bifidobacterium can promote the secretion of mucus by intestinal epithelial cells, and these mucus can provide a protective clothing for the inner wall of the intestine to block the adhesion and invasion of harmful bacteria; Lactobacillus rhamnosus stimulates the proliferation of intestinal epithelial cells, accelerates the repair of damaged intestinal mucosa, makes the intestinal barrier more solid and complete, reduces the risk of pathogenic bacteria taking advantage of the situation to cause diseases, and reduces the burden on the immune system. Meanwhile, the two can jointly regulate the expression of tight junction proteins between intestinal epithelial cells, make the cell connection more tight, prevent harmful substances and allergens in the intestine from penetrating the intestinal wall into the blood, reduce the frequency of abnormal activation of immune cells, and maintain the stability of the intestinal immune system.

[0021] In terms of immune cell activation, Bifidobacterium and Lactobacillus rhamnosus have unique cell wall components such as peptidoglycan and teichoic acid, which are recognized by intestinal immune cells as "danger signals", thereby activating macrophages, dendritic cells and other innate immune cells, prompting them to open the phagocytosis and bactericidal mode; the two bacteria can also synergistically induce these immune cells to release immune regulatory factors and recruit more immune forces. They interact with the gut-associated lymphoid tissue, stimulate the differentiation and maturation of T lymphocytes and B lymphocytes, help the body to build a precise adaptive immune response, so that the immune system can more efficiently respond to various specific pathogens, such as virus infection, helping the body to produce specific antibodies.

[0022] In terms of immune factor regulation, Bifidobacterium and Lactobacillus rhamnosus can promote the secretion of various immune regulatory factors by intestinal epithelial cells and immune cells, including interleukins, interferons, etc. The combined action of the two bacteria can significantly increase the secretion of these immune factors in a short time, regulate immune response in all directions, and enhance the body's anti-inflammatory and anti-viral ability. At the same time, after the immune response is over, they will work together to inhibit the overactive immune factors, avoid persistent inflammation, guide the immune system to return to a stable state, and prevent autoimmune diseases caused by immune imbalance.

[0023] In terms of metabolic product synergies, Bifidobacterium and Lactobacillus rhamnosus ferment dietary fiber in the intestine to produce a large amount of short-chain fatty acids, such as acetic acid, propionic acid, and butyric acid. These short-chain fatty acids not only provide energy for intestinal epithelial cells to maintain cell health, but also are absorbed into the blood to act on immune cells throughout the body, further activating immune function and strengthening the body's ability to resist pathogens. At the same time, both participate in the synthesis of vitamins B, vitamin K, etc., which are essential nutrients for immune cell metabolism and proliferation. Adequate supply helps maintain the activity and number of immune cells, indirectly improving immunity.

[0024] Further, the complex live bacteria powder of the present application and the traditional Chinese medicine extract provided by the present application can synergistically improve the body's immunity. Specifically, Bifidobacterium and Lactobacillus rhamnosus are good at colonizing the intestine, and they promote the secretion of mucus by intestinal epithelial cells to strengthen the physical barrier of the intestine; at the same time, they regulate the tight junction proteins between cells to prevent pathogenic bacteria from leaking. Echinacea polysaccharide and dendrobium polysaccharide can provide nutrients for intestinal epithelial cells, accelerate the repair of damaged cells, and astragalus polysaccharide also helps maintain the metabolic stability of epithelial cells. Working together, the intestinal barrier is impregnable, blocking most pathogenic bacteria from invading and reducing the stress burden on the immune system. And the cell wall components of probiotics act as natural immune signals to activate macrophages, dendritic cells, etc. in the gut-associated lymphoid tissue. The synergistic effect of polygonatum polysaccharide and acanthopanax polysaccharide enhances the sensitivity of these immune cells to probiotic signals, prompting them to release immune regulatory factors more quickly, summoning lymphocytes, etc., and ganoderma peptide regulates the signal transmission efficiency between immune cells, allowing the intestinal immune response to be rapid and orderly.

[0025] Further, the combined stimulation of Bifidobacterium and Lactobacillus rhamnosus causes the phagocytic activity of macrophages and other innate immune cells to soar; Echinacea polysaccharide supplies energy to phagocytes, enhancing their continuous combat capability and enabling them to more efficiently clear pathogens. Astragalus polysaccharide can also induce the proliferation of natural killer cells, broadening the scope of innate immunity. At the same time, probiotics interact with intestinal lymphoid tissue to promote the maturation of T and B lymphocytes. Various polysaccharides provide a material basis for the proliferation and differentiation of lymphocytes, helping them develop into functional effector cells, and ganoderma peptide participates in the regulation of cell differentiation signals, enabling adaptive immunity to accurately respond to different pathogens and produce corresponding antibody and cellular immune responses.

[0026] In addition, the bifidobacterium and lactobacillus rhamnosus induce the secretion of immune factors such as interleukin and interferon by intestinal cells, and the echinacea polysaccharide and dendrobium polysaccharide follow up, greatly increase the secretion amount, and increase the concentration of immune factors in the body in a short time, and enhance the anti-inflammatory and anti-virus ability of the body. The astragalus polysaccharide can also stabilize the intracellular secretion environment in this process, and ensure the continuous and smooth secretion. At the same time, the acanthopanax polysaccharide and ganoderma peptide partners accurately regulate the activity of immune factors at the climax and trough of the immune response, avoid excessive inflammation caused by excessive secretion in the early stage, and prevent the immune factors from inactivating too fast in the later stage, so that the immune defense is long-term stable and efficient.

[0027] The echinacea polysaccharide and polygonatum polysaccharide have antioxidant properties, and can remove free radicals generated in the body due to infection and metabolism; the antioxidant enzymes synthesized by the bifidobacterium and lactobacillus rhamnosus can also reduce the damage of free radicals to immune cells and immune factors, maintain the stability of immune function, and ganoderma peptide repairs the cell membrane damaged by free radicals, and helps the reproduction of immune cells.

[0028] In a second aspect, the present application provides a preparation method of the ganoderma peptide composition, comprising the following steps:

[0029] (1) The ganoderma lucidum is crushed and sieved, mixed with water, inactivated after enzymolysis, centrifuged, dried and obtained as ganoderma peptide:

[0030] (2) The dendrobium officinale is crushed and sieved, mixed with water, soaked, extracted by heating, centrifuged and filtered to obtain a liquid phase, the liquid phase is vacuum concentrated, dissolved in ethanol, placed, centrifuged and dried to obtain dendrobium polysaccharide;

[0031] (3) The polygonatum sibiricum is crushed and sieved, mixed with water, soaked, extracted by heating, centrifuged and filtered to obtain a liquid phase, the liquid phase is vacuum concentrated, dissolved in ethanol, placed, centrifuged and dried to obtain polygonatum polysaccharide;

[0032] (4) The astragalus membranaceus is crushed and sieved, mixed with water, soaked, extracted by heating, centrifuged and filtered to obtain a liquid phase, the liquid phase is vacuum concentrated, dissolved in ethanol, placed, centrifuged and dried to obtain astragalus polysaccharide;

[0033] (5) The acanthopanax root and rhizome are crushed and sieved, mixed with water, soaked, extracted by heating, centrifuged and filtered to obtain a liquid phase, the liquid phase is vacuum concentrated, dissolved in ethanol, placed, centrifuged and dried to obtain acanthopanax polysaccharide;

[0034] (6) The echinacea is mixed with water, the pH is adjusted to 3-5, inactivated after enzymolysis, centrifuged and dried to obtain echinacea polysaccharide;

[0035] (7) The bifidobacterium strain is activated and cultured to a bacterial density of 2 x 10 10CFU / ml-3 x 10 20 After 2-3 generations of continuous culture, the obtained bacterial suspension is freeze-dried to obtain Ganoderma lucidum viable bacterial powder:

[0036] (8) The Lactobacillus rhamnosus strain is activated and cultured to a bacterial density of 2 x 10 10 CFU / ml-3 x 10 20 After 2-3 generations of continuous culture, the obtained bacterial suspension is freeze-dried to obtain Ganoderma lucidum viable bacterial powder:

[0037] (9) The Lactobacillus rhamnosus viable bacterial powder, the Bifidobacterium viable bacterial powder, the ganoderma peptide, the dendrobium officinale polysaccharide, the polygonatum sibiricum polysaccharide, the astragalus polysaccharide, the acanthopanax polysaccharide, and the purple cone flower polysaccharide are mixed to obtain a ganoderma peptide composition.

[0038] In a preferred embodiment, in step (1), the sieving uses a screen of 200-400 mesh.

[0039] In a preferred embodiment, in step (1), the Ganoderma lucidum and water are in a mass ratio of 1: (50-100).

[0040] In a preferred embodiment, in step (1), the enzymolysis conditions are:

[0041] The complex enzyme composed of hemicellulase and papain in a weight ratio of 1:1 is used for enzymolysis, the enzymolysis time is 60-100 min, the enzymolysis temperature is 40-50℃, and the addition amount of the complex enzyme is 1wt%-3wt% of the Ganoderma lucidum powder.

[0042] In a preferred embodiment, in step (2), the sieving uses a screen of 200-400 mesh.

[0043] In a preferred embodiment, in step (2), the dendrobium officinale and water are in a mass ratio of 1: (50-100).

[0044] In a preferred embodiment, in step (2), the soaking time is 2-4h.

[0045] In a preferred embodiment, in step (2), the temperature rising extraction conditions are: rising the temperature to 60-100℃ and keeping the temperature for 1.5-3h.

[0046] In a preferred embodiment, in step (2), the volume of the added ethanol is 2-4 times of the volume of the water.

[0047] In a preferred embodiment, in step (3), the sieving uses a screen of 200-400 mesh.

[0048] In a preferred embodiment, in step (3), the mass ratio of the Huangqi to water is 1:(50-100).

[0049] In a preferred embodiment, in step (3), the soaking time is 2-4h.

[0050] In a preferred embodiment, in step (3), the temperature rising extraction condition is: rising the temperature to 60-100℃ and keeping for 1.5-3h.

[0051] In a preferred embodiment, in step (3), the volume of the added ethanol is 2-4 times of the volume of the water.

[0052] In a preferred embodiment, in step (4), the sifting uses a 200-400 mesh screen.

[0053] In a preferred embodiment, in step (4), the mass ratio of the Huangqi to water is 1:(50-100).

[0054] In a preferred embodiment, in step (4), the soaking time is 2-4h.

[0055] In a preferred embodiment, in step (4), the temperature rising extraction condition is: rising the temperature to 60-100℃ and keeping for 1.5-3h.

[0056] In a preferred embodiment, in step (4), the volume of the added ethanol is 2-4 times of the volume of the water.

[0057] In a preferred embodiment, in step (5), the sifting uses a 200-400 mesh screen.

[0058] In a preferred embodiment, in step (5), the mass ratio of the Ciwujia root and rhizome to water is 1:(50-100).

[0059] In a preferred embodiment, in step (5), the soaking time is 2-4h.

[0060] In a preferred embodiment, in step (5), the temperature rising extraction condition is: rising the temperature to 60-100℃ and keeping for 1.5-3h.

[0061] In a preferred embodiment, in step (5), the volume of the added ethanol is 2-4 times of the volume of the water.

[0062] In a preferred embodiment, in step (6), the mass ratio of the Chrysanthemum morifolium Ramat to water is 1:(50-100).

[0063] In a preferred embodiment, in step (6), the enzymolysis conditions are as follows:

[0064] The enzymolysis is performed by using pectinase, the pectinase is added in an amount of 1wt%-4wt% of the purple cone flower, the enzymolysis temperature is 40-60 DEG C, and the enzymolysis time is 2-4h.

[0065] In a preferred embodiment, in steps (7) and (8), the culture medium used in the culture is MRS culture medium, and the raw materials in the MRS culture medium include, with water 1000ml as a relative amount, 5-15g of proteose peptone, 2-5g of yeast extract, 8-15g of beef extract, 15-20g of glucose, 2-4g of sodium acetate, 1-2g of diammonium citrate, 1-2ml of Tween 80, 0.2-0.4g of magnesium sulfate, 0.01-0.04g of manganese sulfate, 2-4g of dipotassium hydrogen phosphate, 15-20g of agar, 3-5g of ganoderma peptide, 1-2g of purple cone flower polysaccharide, 0.8-1.8g of polygonatum polysaccharide, 0.5-2.5g of dendrobium polysaccharide, 0.9-1.6g of astragalus polysaccharide, and 1.2-2.4g of acanthopanax polysaccharide.

[0066] In a third aspect, the application provides a use of the ganoderma peptide composition as described above in the preparation of an immune-enhancing drug and / or an auxiliary immune-enhancing drug.

[0067] Compared with the prior art, the application has the following advantages and beneficial effects:

[0068] 1. The ganoderma peptide and the polysaccharide of traditional Chinese medicine extract are compounded to improve the immunity from the aspects of immune cell activation, immune factor regulation, antioxidant and anti-stress, and intestinal flora optimization.

[0069] 2. The probiotic live powder is added to the composition, and the probiotic live powder and the polysaccharide of traditional Chinese medicine extract synergistically improve the immunity of the body, and the problem that the polysaccharide of traditional Chinese medicine extract is difficult to be fully decomposed and absorbed is solved.

[0070] 3. The probiotic is further limited to bifidobacterium and lactobacillus rhamnosus, and the two probiotics synergistically improve the function of the composition in regulating immunity in terms of intestinal barrier reinforcement, immune cell activation, immune factor regulation, and metabolic product synergism.

[0071] 4. The live bacteria powder compounded in this invention, together with the traditional Chinese medicine extracts provided by this invention, can synergistically enhance the body's immunity. Specifically, Bifidobacterium and Lactobacillus rhamnosus are adept at colonizing the intestines, prompting intestinal epithelial cells to secrete mucus, thus strengthening the physical barrier of the intestines; at the same time, they regulate intercellular tight junction proteins to prevent pathogen leakage. Echinacea polysaccharides and Dendrobium polysaccharides provide nutrients to intestinal epithelial cells, accelerating the repair of damaged cells, while Astragalus polysaccharides also help maintain the metabolic stability of epithelial cells. Through multiple approaches, the intestinal barrier becomes indestructible, blocking the invasion of most pathogens and reducing the stress burden on the immune system. Furthermore, the cell wall components of probiotics act as natural immune signals, activating macrophages and dendritic cells in gut-associated lymphoid tissue. Polygonatum polysaccharides and Acanthopanax senticosus polysaccharides work synergistically to enhance the sensitivity of these immune cells to probiotic signals, prompting them to release immunomodulatory factors more quickly and recruit lymphocytes, while Ganoderma lucidum peptides regulate the efficiency of signal transmission between immune cells, allowing the intestinal immune response to unfold rapidly and in an orderly manner. Detailed Implementation

[0072] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0073] Unless otherwise specified, all reagents and raw materials used in this invention are commercially available.

[0074] Lactobacillus rhamnosus, MP108, purchased from Jinqiao Biotechnology Co., Ltd.

[0075] Bifidobacterium, BB-12, purchased from Chr. Hansen, Denmark.

[0076] The culture medium used was MRS medium, with 1000 mL of water as the relative volume. The raw materials of the MRS medium included: 10 g peptone; 5 g yeast extract; 12 g beef extract; 18 g glucose; 3 g sodium acetate; 1.5 g diammonium citrate; 1.5 mL Tween 80; 0.3 g magnesium sulfate; 0.02 g manganese sulfate; 3 g dipotassium hydrogen phosphate; 18 g agar; 5 g Ganoderma lucidum peptide; 2 g echinacea polysaccharide; 1.8 g polygonatum polysaccharide; 2.5 g dendrobium polysaccharide; 1.6 g astragalus polysaccharide; and 2.4 g eleutherococcus senticosus polysaccharide.

[0077] Example 1

[0078] This embodiment provides a method for preparing a Ganoderma lucidum peptide composition, including the following steps:

[0079] (1) 1 part of Ganoderma lucidum is crushed to pass through a 200-mesh sieve and mixed with 70 parts of water, and then subjected to enzymatic hydrolysis using a complex enzyme composed of hemicellulase and papain at a weight ratio of 1:1, the enzymatic hydrolysis time is 80 min, the enzymatic hydrolysis temperature is 45℃, the addition amount of the complex enzyme is 1.5 wt% of the Ganoderma lucidum powder, and then inactivated, centrifuged, and dried to obtain Ganoderma lucidum peptides:

[0080] (2) 1 part of Dendrobium officinale is crushed to pass through a 200-mesh sieve and mixed with 70 parts of water, soaked for 2.5 h, and then subjected to extraction by increasing the temperature, and centrifuged and filtered to obtain a liquid phase, the liquid phase is vacuum concentrated, dissolved in ethanol, and then subjected to standing, centrifugation, and drying to obtain Dendrobium officinale polysaccharides

[0081] (3) 1 part of Polygonatum sibiricum is crushed to pass through a 200-mesh sieve and mixed with 70 parts of water, soaked for 2.5 h, and then subjected to extraction by increasing the temperature to 80℃ and maintaining for 3 h, and centrifuged and filtered to obtain a liquid phase, the liquid phase is vacuum concentrated, dissolved in 150 parts of ethanol, and then subjected to standing, centrifugation, and drying to obtain Polygonatum sibiricum polysaccharides;

[0082] (4) 1 part of Astragalus membranaceus is crushed to pass through a 200-mesh sieve and mixed with 70 parts of water, soaked for 2.5 h, and then subjected to extraction by increasing the temperature to 80℃ and maintaining for 3 h, and centrifuged and filtered to obtain a liquid phase, the liquid phase is vacuum concentrated, dissolved in 150 parts of ethanol, and then subjected to standing, centrifugation, and drying to obtain Astragalus membranaceus polysaccharides;

[0083] (5) 1 part of Acanthopanax senticosus root and rhizome is crushed to pass through a 200-mesh sieve and mixed with 70 parts of water, soaked for 2.5 h, and then subjected to extraction by increasing the temperature to 80℃ and maintaining for 3 h, and centrifuged and filtered to obtain a liquid phase, the liquid phase is vacuum concentrated, dissolved in 150 parts of ethanol, and then subjected to standing, centrifugation, and drying to obtain Acanthopanax senticosus polysaccharides;

[0084] (6) 1 part of Echinacea purpurea is mixed with 70 parts of water, and the pH is adjusted to 3.5, and then subjected to enzymatic hydrolysis using pectinase, the addition amount of the pectinase is 2 wt% of the Echinacea purpurea, the enzymatic hydrolysis temperature is 50℃, and the enzymatic hydrolysis time is 2.5 h, and then subjected to inactivation, centrifugation, and drying to obtain Echinacea purpurea polysaccharides;

[0085] (7) Bifidobacterium strain freeze-dried powder is activated and cultured to a bacterial density of 2.5 x 10 20 CFU / ml, and then cultured for 2 more generations, and the obtained bacterial suspension is freeze-dried to obtain Bifidobacterium live bacteria powder:

[0086] (8) Lactobacillus rhamnosus strain freeze-dried powder is activated and cultured to a bacterial density of 2.5 x 10 20 CFU / ml, and then cultured for 2 more generations, and the obtained bacterial suspension is freeze-dried to obtain Lactobacillus rhamnosus live bacteria powder;

[0087] (9) mixing 13 parts of Lactobacillus rhamnosus live bacteria powder, 13 parts of Bifidobacterium live bacteria powder, 33 parts of ganoderma peptide, 10 parts of dendrobium polysaccharide, 10 parts of polygonatum polysaccharide, 11 parts of astragalus polysaccharide, 15 parts of eleutherococcus senticosus polysaccharide, and 12 parts of guayule polysaccharide to obtain a ganoderma peptide composition.

[0088] Example 2

[0089] The present embodiment provides a preparation method of a ganoderma peptide composition, comprising the following steps:

[0090] (1) according to mass fraction, 1 part of Ganoderma lucidum powder is crushed through a 200-mesh sieve and mixed with 90 parts of water, and then subjected to enzymolysis by using a complex enzyme composed of hemicellulase and papain at a weight ratio of 1:1, the enzymolysis time is 80 min, the enzymolysis temperature is 45℃, the addition amount of the complex enzyme is 1.5wt% of the Ganoderma lucidum powder, then inactivated, centrifuged, and dried to obtain ganoderma peptide:

[0091] (2) according to mass fraction, 1 part of Dendrobium officinale Kimura et Migo is crushed through a 200-mesh sieve and mixed with 90 parts of water, soaked for 2.5h, and then subjected to extraction by heating, centrifuged and filtered to obtain a liquid phase, the liquid phase is vacuum concentrated and dissolved in ethanol, and then subjected to standing, centrifugation and drying to obtain dendrobium polysaccharide;

[0092] (3) according to mass fraction, 1 part of Polygonatum sibiricum is crushed through a 200-mesh sieve and mixed with 90 parts of water, soaked for 2.5h, and then subjected to extraction by heating to 80℃ and keeping for 3h, centrifuged and filtered to obtain a liquid phase, the liquid phase is vacuum concentrated and dissolved in 150 parts of ethanol, and then subjected to standing, centrifugation and drying to obtain polygonatum polysaccharide;

[0093] (4) according to mass fraction, 1 part of Astragalus membranaceus is crushed through a 200-mesh sieve and mixed with 90 parts of water, soaked for 2.5h, and then subjected to extraction by heating to 80℃ and keeping for 3h, centrifuged and filtered to obtain a liquid phase, the liquid phase is vacuum concentrated and dissolved in 150 parts of ethanol, and then subjected to standing, centrifugation and drying to obtain astragalus polysaccharide;

[0094] (5) according to mass fraction, 1 part of Acanthopanax senticosus root and rhizome is crushed through a 200-mesh sieve and mixed with 90 parts of water, soaked for 2.5h, and then subjected to extraction by heating to 80℃ and keeping for 3h, centrifuged and filtered to obtain a liquid phase, the liquid phase is vacuum concentrated and dissolved in 150 parts of ethanol, and then subjected to standing, centrifugation and drying to obtain eleutherococcus senticosus polysaccharide;

[0095] (6) according to mass fraction, 1 part of guayule is mixed with 90 parts of water, and the pH is adjusted to 3.5, and then subjected to enzymolysis by using pectinase, the addition amount of the pectinase is 2wt% of the guayule, the enzymolysis temperature is 50℃, the enzymolysis time is 2.5h, inactivated, centrifuged, and dried to obtain guayule polysaccharide;

[0096] (7) the strain of Bifidobacterium is activated and cultured to a bacterial density of 2.5×10 20After 2 generations of continuous culture, the obtained bacterial suspension was freeze-dried to obtain bifidobacterium viable powder:

[0097] (8) The strain of Lactobacillus rhamnosus was activated and cultured to a bacterial density of 2.5 x 10 20 After 2 generations of continuous culture, the obtained bacterial suspension was freeze-dried to obtain Lactobacillus rhamnosus viable powder;

[0098] (9) 20 parts of Lactobacillus rhamnosus viable powder, 20 parts of bifidobacterium viable powder, 46 parts of ganoderma peptide, 18 parts of dendrobium polysaccharide, 16 parts of polygonatum polysaccharide, 14 parts of astragalus polysaccharide, 22 parts of eleutherococcus polysaccharide, and 18 parts of guayule polysaccharide were mixed to obtain a ganoderma peptide composition.

[0099] Comparative Example 1

[0100] The difference between this comparative example and Example 1 is that no Lactobacillus rhamnosus viable powder and bifidobacterium viable powder are added.

[0101] Comparative Example 2

[0102] The difference between this comparative example and Example 1 is that the probiotic powder only contains 26 parts of Lactobacillus rhamnosus viable powder.

[0103] Comparative Example 3

[0104] The difference between this comparative example and Example 1 is that the probiotic powder only contains 26 parts of bifidobacterium viable powder.

[0105] Comparative Example 4

[0106] The difference between this comparative example and Example 1 is that no dendrobium polysaccharide is added, and the mass fraction of ganoderma peptide is changed to 43 parts.

[0107] Comparative Example 5

[0108] The difference between this comparative example and Example 1 is that no polygonatum polysaccharide is added, and the mass fraction of ganoderma peptide is changed to 43 parts.

[0109] Comparative Example 6

[0110] The difference between this comparative example and Example 1 is that no astragalus polysaccharide is added, and the mass fraction of ganoderma peptide is changed to 44 parts.

[0111] Comparative Example 7

[0112] The difference between this comparative example and Example 1 is that no eleutherococcus polysaccharide is added, and the mass fraction of ganoderma peptide is changed to 48 parts.

[0113] Comparative Example 8

[0114] The difference between this comparative example and Example 1 is that no guayule polysaccharide is added, and the mass fraction of ganoderma peptide is changed to 45 parts.

[0115] Animal experiment:

[0116] 1. Animal grouping: 200 SPF level SD rats (body weight 160±10g) of 6-8 weeks old were adaptively fed for 7 days, and then randomly divided into normal group, example 1 group, and comparative example 8 group, 20 rats in each group.

[0117] 2. Modeling and administration of animals: the example 1 group was given the ganoderma lucidum peptide composition prepared in example 1 (0.1g each time, 2 times a day), the comparative example 8 group was given the composition obtained in comparative example 1-8 (0.1g each time, 2 times a day), the normal group was not operated, and was continuously operated for 21 days, on the 22nd day, except for the normal group, the rats in each group were intraperitoneally injected with cyclophosphamide 60mg / kg, continuously for 4 days, and the normal group was given the same amount of normal saline. On the 35th day, all rats were anesthetized and killed by carbon dioxide asphyxia, and blood was taken.

[0118] 3. Test: the blood of rats in each test group was centrifuged at 13400rpm for 10 minutes at 4℃, and the supernatant was taken for immune index analysis. The concentrations of interferon-γ (IFN-γ), interleukin-4 (IL-4), immunoglobulin M (IgM) and immunoglobulin G (IgG) in the serum of rats were determined by enzyme-linked immunosorbent assay (ELISA), and the results are shown in table 1.

[0119] Table 1 analysis of immune index of rats

[0120]

[0121]

[0122] From the performance test results, it can be seen that example 1 improves the immune function of rats better, which is mainly because the synergistic effect of the compounded traditional Chinese medicine extract and the live probiotic powder improves the effect of the composition on immunity.

[0123] The comparative examples are significantly worse than the examples in the corresponding performance test because they do not use the necessary technical solutions, which better proves the irreplaceability of the specific technical solutions of the present application for achieving the technical effect and solving the technical problem.

[0124] The above is a preferred embodiment of the present application. It should be noted that for ordinary skilled persons in the art, without departing from the principles of the present application, a number of improvements and refinements can be made, which should also be considered as the protection scope of the present application.

Claims

1. A method for preparing ganoderma peptide composition, characterized in that, The ganoderma lucidum peptide composition comprises the following components in parts by mass: ganoderma lucidum peptide 30-50 parts, echinacea purpurea polysaccharide 10-20 parts, polygonatum sibiricum polysaccharide 8-18 parts, dendrobium polysaccharide 5-25 parts, astragalus polysaccharide 9-16 parts, acanthopanax polysaccharide 12-24 parts, 20-50 parts of probiotic live bacteria powder, and the number of live bacteria in the probiotic live bacteria powder is 4 x 10 10 CFU / g-6 x 10 20 CFU / g; The probiotic viable powder is a mixture of viable Bacillus subtilis powder and viable Lactobacillus rhamnosus powder, and the mass ratio of the viable Bacillus subtilis powder and the viable Lactobacillus rhamnosus powder is 1:1; The viable bacteria number in the bifidobacterium viable bacteria powder is 2 x 10 10 CFU / g-3 x 10 20 CFU / g; The viable cell count in the Lactobacillus rhamnosus powder is 2 x 10 10 CFU / g-3 x 10 20 CFU / g; The preparation method comprises the following steps: (1) After the Ganoderma lucidum is crushed and sieved, the Ganoderma lucidum is mixed with water, inactivated after enzymolysis, and dried after centrifugal separation to obtain Ganoderma lucidum peptides; the enzymolysis conditions are as follows: a composite enzyme composed of hemicellulase and papain at a weight ratio of 1:1 is used for enzymolysis; (2) After the Dendrobium officinale is crushed and sieved, the Dendrobium officinale is mixed with water, soaked, extracted by heating, and centrifuged and filtered to obtain a liquid phase; the liquid phase is vacuum concentrated, dissolved in ethanol, and dried after centrifugal separation to obtain Dendrobium officinale polysaccharides; (3) After the Polygonatum sibiricum is crushed and sieved, the Polygonatum sibiricum is mixed with water, soaked, extracted by heating, and centrifuged and filtered to obtain a liquid phase; the liquid phase is vacuum concentrated, dissolved in ethanol, and dried after centrifugal separation to obtain Polygonatum sibiricum polysaccharides; (4) After the Astragalus membranaceus is crushed and sieved, the Astragalus membranaceus is mixed with water, soaked, extracted by heating, and centrifuged and filtered to obtain a liquid phase; the liquid phase is vacuum concentrated, dissolved in ethanol, and dried after centrifugal separation to obtain Astragalus membranaceus polysaccharides; (5) After the Acanthopanax senticosus root and rhizome are crushed and sieved, the Acanthopanax senticosus root and rhizome are mixed with water, soaked, extracted by heating, and centrifuged and filtered to obtain a liquid phase; the liquid phase is vacuum concentrated, dissolved in ethanol, and dried after centrifugal separation to obtain Acanthopanax senticosus polysaccharides; (6) The Echinacea purpurea is mixed with water, and the pH is adjusted to 3-5; after enzymolysis, the Echinacea purpurea is inactivated, and dried after centrifugal separation to obtain Echinacea purpurea polysaccharides; the enzymolysis conditions are as follows: pectinase is used for enzymolysis; (7) The freeze-dried powder of Bifidobacterium is activated and cultured to a bacterial density of 2 x 10 10 CFU / ml-3 x 10 20 CFU / ml, and then cultured for 2-3 generations. The obtained bacterial suspension is freeze-dried to obtain the live Bifidobacterium powder: (8) The Lactobacillus rhamnosus strain is activated and cultured to a bacterial density of 2 x 1010 CFU / ml-3 x 1010 CFU / ml 10 CFU / ml-3 x 1010 CFU / ml 20 CFU / ml, and then the obtained bacterial suspension is freeze-dried to obtain the Lactobacillus rhamnosus live bacterial powder. (9) The viable Lactobacillus rhamnosus powder, the viable Bacillus subtilis powder, the Ganoderma lucidum peptides, the Dendrobium officinale polysaccharides, the Polygonatum sibiricum polysaccharides, the Astragalus membranaceus polysaccharides, the Acanthopanax senticosus polysaccharides and the Echinacea purpurea polysaccharides are mixed to obtain Ganoderma lucidum peptide compositions; In steps (7) and (8), the culture medium used for culture is an MRS culture medium, and water 1000ml is used as a relative amount; raw materials in the MRS culture medium include: protein peptone 5-15g; yeast paste 2-5g; beef paste 8-15g; glucose 15-20g; sodium acetate 2-4g; diammonium citrate 1-2g; Tween 80 1-2ml; magnesium sulfate 0.2-0.4g; manganese sulfate 0.01-0.04g; dipotassium hydrogen phosphate 2-4g; agar 15-20g; Ganoderma lucidum peptides 3-5g, Echinacea purpurea polysaccharides 1-2g, Polygonatum sibiricum polysaccharides 0.8-1.8g, Dendrobium officinale polysaccharides 0.5-2.5g, Astragalus membranaceus polysaccharides 0.9-1.6g, and Acanthopanax senticosus polysaccharides 1.2-2.4g.

2. The method of claim 1, wherein the Ganoderma lucidum peptide composition is prepared by the steps of: In step (1), the sieving uses a screen of 200-400 meshes; And / or, in step (1), the mass ratio of the Ganoderma lucidum to water is 1:(50-100); And / or, in step (1), the enzymolysis conditions are as follows: a composite enzyme composed of hemicellulase and papain at a weight ratio of 1:1 is used for enzymolysis, the enzymolysis time is 60-100min, the enzymolysis temperature is 40-50℃, and the addition amount of the composite enzyme is 1 wt%-3 wt% of the Ganoderma lucidum powder.

3. The method of claim 2, wherein the Ganoderma lucidum peptide composition is prepared by the steps of: In step (2), the sieving uses a screen of 200-400 meshes; And / or, in step (2), the mass ratio of the Dendrobium candidum to water is 1: (50-100); And / or, in step (2), the soaking time is 2-4h; And / or, in step (2), the temperature rising extraction condition is: rising to 60-100℃ and keeping for 1.5-3h; And / or, in step (2), the volume of the added ethanol is 2-4 times of the volume of the water.

4. The method of claim 3, wherein the Ganoderma lucidum peptide composition is prepared by the steps of: In step (3), the sieving uses a 200-400 mesh sieve; And / or, in step (3), the mass ratio of the Polygonatum sibiricum to water is 1: (50-100); And / or, in step (3), the soaking time is 2-4h; And / or, in step (3), the temperature rising extraction condition is: rising to 60-100℃ and keeping for 1.5-3h; And / or, in step (3), the volume of the added ethanol is 2-4 times of the volume of the water.

5. The method of claim 3, wherein the Ganoderma lucidum peptide composition is prepared by the steps of: In step (4), the sieving uses a 200-400 mesh sieve; And / or, in step (4), the mass ratio of the Astragalus membranaceus to water is 1: (50-100); And / or, in step (4), the soaking time is 2-4h; And / or, in step (4), the temperature rising extraction condition is: rising to 60-100℃ and keeping for 1.5-3h; And / or, in step (4), the volume of the added ethanol is 2-4 times of the volume of the water.

6. The method of claim 3, wherein the Ganoderma lucidum peptide composition is prepared by the steps of: In step (5), the sieving uses a 200-400 mesh sieve; And / or, in step (5), the mass ratio of the Acanthopanax senticosus root and rhizome to water is 1: (50-100); And / or, in step (5), the soaking time is 2-4h; And / or, in step (5), the temperature rising extraction condition is: rising to 60-100℃ and keeping for 1.5-3h; And / or, in step (5), the volume of the added ethanol is 2-4 times of the volume of the water. And / or, in step (6), the mass ratio of the Echinacea purpurea to water is 1: (50-100); And / or, in step (6), the enzymolysis condition is: using pectinase for enzymolysis, the added amount of the pectinase is 1 wt%-4 wt% of the Echinacea purpurea, the enzymolysis temperature is 40-60℃, and the enzymolysis time is 2-4h.

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