Immobilized enzyme reactor for preparing low-carbon-chain triglyceride

Abstract

The invention discloses an immobilized enzyme reactor for preparing low-carbon-chain triglyceride, and relates to the technical field of bioreactors, the immobilized enzyme reactor comprises a reactor body, a bearing ball carrying enzyme, a feeding pipe, a discharging pipe, a conveying channel, a conveying mechanism and a collector; a filter pipe is arranged in the reactor body, the upper end of the filter pipe is communicated with the conveying channel through a feeding pipe, the lower end of the filter pipe is communicated with a collector through a discharging pipe, and the lower end of the collector is communicated with the conveying channel; unused bearing balls are contained in the collector, when the immobilized enzyme needs to be recycled, the vibrator on the filter pipe vibrates the filter pipe, the bearing balls in the filter pipe roll into the collector from the discharging pipe under the combined action of vibration and gravity, and the unused bearing balls in the collector are moved to the feeding pipe through the conveying mechanism and finally enter the filter pipe; an operator can open the cover plate of the collector and take out the used bearing balls from the discharge port, so that the effect of recycling the immobilized enzyme is realized.

Description

Technical Field

[0001] The present invention relates to the technical field of bioreactors, and particularly relates to an immobilized enzyme reactor for preparing low-carbon chain triglycerides. Background Art

[0002] Low-carbon chain triglycerides are compounds formed by the esterification of glycerol and medium-chain fatty acids, which are liquid at room temperature and are widely used in the fields of food, medicine, and industry. Low-carbon chain triglycerides are usually prepared through a reactor. The reactor stirs and mixes the substrates, and the reaction temperature is controlled at 50°C to 70°C. An immobilized enzyme is provided as a catalyst in the reactor. Although the immobilized enzyme does not directly participate in the reaction, due to certain factors, such as high temperature, erosion by organic solvents, and physical abrasion of the enzyme by the fluid, the activity of the enzyme will gradually decrease. Therefore, it is necessary to regularly supplement or replace the immobilized enzyme.

[0003] In the existing immobilized enzyme reactors, the immobilized enzyme is usually placed in a filter cartridge of the reactor, resulting in inconvenient replacement and recovery of the immobilized enzyme. Summary of the Invention

[0004] The main object of the present invention is to provide an immobilized enzyme reactor for preparing low-carbon chain triglycerides, aiming to solve the technical problem of inconvenient recovery of the immobilized enzyme in the existing immobilized enzyme reactors.

[0005] To achieve the above object, the present invention provides an immobilized enzyme reactor for preparing low-carbon chain triglycerides, comprising:

[0006] A reactor body, the reactor body is provided with a drain port; a spiral filter tube is fixed inside the reactor body, and the filter tube is provided with a plurality of flow holes; a vibrator is provided on the outer side wall of the filter tube;

[0007] A plurality of carrier balls, the carrier balls are provided with honeycomb-shaped pores, and the inner surface of the pores is attached with an enzyme. The carrier balls are located inside the filter tube;

[0008] A recovery assembly, including a feed pipe, a discharge pipe, a conveying channel, a conveying mechanism, and a collector; one end of the feed pipe is communicated with the upper end of the filter tube, and the other end of the feed pipe is communicated with the conveying channel; one end of the discharge pipe is communicated with the lower end of the filter tube, and the other end of the discharge pipe is communicated with the upper part inside the collector; a discharge port and a cover plate for sealing the discharge port are provided at the upper end of the collector. The lower end of the collector is in a funnel shape and is communicated with the lower part of the conveying channel. Valves are provided at the lower end of the collector, the feed pipe, and the discharge pipe; the diameters of the feed pipe, the discharge pipe, and the discharge port are all larger than the diameter of the carrier ball; the conveying mechanism is located inside the conveying channel and is used to convey the carrier balls close to the discharge port to the feed pipe.

[0009] Optionally, it further includes a first liquid storage tank, a first pipe, a first pump body and an ultrasonic generator; the first liquid storage tank is filled with a first reaction liquid inside; both ends of the first pipe are respectively communicated with the first liquid storage tank and the collector; the first pump body is arranged on the first pipe; the ultrasonic generator is arranged on the collector; the collector is provided with a drain pipe.

[0010] Optionally, it further includes a second liquid storage tank, a second pipe, a second pump body, a heater, a vibration assembly and a plurality of flexible pipes; the second liquid storage tank is filled with a second reaction liquid inside; both ends of the second pipe are respectively communicated with the second liquid storage tank and the collector; the second pump body is arranged on the second pipe; the collector is arranged on the vibration assembly; the discharge pipe, the conveying channel, the first pipe and the second pipe are respectively communicated with the collector through the flexible pipes; the heater is arranged on the collector.

[0011] Optionally, the vibration assembly includes a base, a mounting plate, a crank and connecting rod mechanism and two pairs of swing rods; the two pairs of swing rods are respectively located on both sides of the mounting plate, and each pair of swing rods is distributed in an inverted "eight" character structure. One end of the swing rod is rotatably connected to the base, and the other end of the swing rod is rotatably connected to the mounting plate; the collector is arranged on the mounting plate; the crank and connecting rod mechanism is arranged on the base and is used to drive the mounting plate to vibrate.

[0012] Optionally, it further includes a vacuum pump and a third pipe; both ends of the third pipe are respectively communicated with the vacuum pump and the collector.

[0013] Optionally, the conveying mechanism includes a first motor, a conveyor belt, a driving roller and a plurality of driven rollers; both the driving roller and the driven rollers are rotatably connected to the conveying channel, and the conveyor belt is drivingly connected to the driving roller and the driven rollers; the first motor is arranged on the conveying channel and is used to drive the driving roller to rotate; the conveyor belt is provided with a plurality of stoppers along the length direction of the conveyor belt, and the distance between two adjacent stoppers forms a clamping groove for clamping the carrier ball.

[0014] Optionally, a square groove penetrating through the stopper is formed in the middle of the stopper, the square groove divides the stopper into two clamping blocks arranged along the width direction of the conveyor belt, and the width of the square groove is greater than the radius of the carrier ball; the feed pipe is provided with a receiving groove for cooperating with the clamping blocks.

[0015] Optionally, it further includes a second motor, a reversing transmission mechanism and a stirrer; the stirrer is rotatably connected to the reactor body, and the stirrer and the filter tube share the same central axis; the second motor is arranged on the reactor body; the reversing transmission mechanism is used to transmit the power of the second motor to the stirrer and drive the stirrer to rotate forward and backward.

[0016] Optionally, the reversing transmission mechanism includes a gear, a rack and a driving crank; the reactor body is provided with a connecting plate; the driving crank is rotatably connected to the connecting plate, and the driving crank is provided with a traction shaft; the second motor is arranged on the connecting plate and used to drive the driving crank to rotate; the rack is slidably arranged on the connecting plate and the sliding direction is parallel to the length direction of the rack; the rack is provided with a mounting block, and the mounting block is provided with a sliding hole whose length direction is perpendicular to the length direction of the rack, and the sliding hole cooperates with the traction shaft; the gear is rotatably arranged on the connecting plate and is coaxially connected to the stirrer, and the gear is in transmission engagement with the rack.

[0017] Optionally, a sampling port and a valve are arranged on the feed pipe, and the sampling port is located between the reactor body and the valve.

[0018] The technical solution of the present invention provides an immobilized enzyme reactor for preparing low-carbon chain triglycerides. The substrate liquid in the reactor body can pass through the flow holes of the filter tube and contact the carrier balls, so that the enzymes on the carrier balls can catalyze the substrate liquid to promote the reaction; the collector is filled with unused carrier balls. When it is necessary to recycle the immobilized enzyme, the reactor body discharges the substrate liquid from the drain port. At this time, the valves on the lower end of the collector, the feed pipe and the discharge pipe are opened, and the vibrator vibrates the filter tube. The carrier balls in the filter tube roll into the collector from the discharge pipe under the combined action of vibration and gravity. The unused carrier balls in the collector are moved to the feed pipe through the transmission mechanism and finally enter the filter tube; the operator can open the cover plate of the collector and take out the used carrier balls from the discharge port, thereby achieving the effect of recycling the immobilized enzyme. Description of the Drawings

[0019] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for the description of the embodiments or the prior art. Obviously, the following drawings are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on the structures shown in these drawings.

[0020] Figure 1 It is a schematic structural diagram of the immobilized enzyme reactor for preparing low-carbon chain triglycerides in the embodiment of the present invention;

[0021] Figure 2 Schematic structural diagram of the collector and vibration assembly according to an embodiment of the present invention;

[0022] Figure 3 Partial schematic diagram of the feed pipe and conveying mechanism according to an embodiment of the present invention;

[0023] Figure 4 Schematic structural diagram of the commutation transmission mechanism according to an embodiment of the present invention;

[0024] Figure 5 Schematic structural diagram of the stirrer according to an embodiment of the present invention.

[0025] Icon: 101, reactor body; 102, liquid discharge port; 103, filter tube; 1031, circulation hole; 104, vibration motor; 105, toggle rod; 106, second motor; 107, commutation transmission mechanism; 1071, gear; 1072, rack; 1073, driving crank; 1074, mounting block; 10741, sliding hole; 1075, traction shaft; 108, stirrer; 1081, connecting shaft; 1082, blade; 1083, fixing ring; 109, connecting plate;

[0026] 200, bearing ball; 301, feed pipe; 3011, receiving groove; 3012, sampling port; 302, discharge pipe;

[0027] 303, conveying channel; 3041, conveyor belt; 3042, driving roller; 3043, driven roller; 3044, stop block; 30441, clamping block; 305, collector; 3051, cover plate; 3052, drain pipe; 401, first liquid storage tank; 402, first pipe; 403, first pump body; 404, ultrasonic generator; 501, second liquid storage tank; 502, second pipe; 503, second pump body; 504, heater; 5051, base; 5052, mounting plate; 5053, crank - connecting rod mechanism; 5054, swing rod; 506, flexible pipe; 601, vacuum pump; 602, third pipe. Detailed implementation manners

[0028] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without making creative efforts belong to the scope of protection of the present invention.

[0029] It should be noted that all directional indications (such as up, down, left, right, front, back, etc.) in the embodiments of the present invention are only used to explain the relative positional relationship, movement conditions, etc. between components in a specific posture (as shown in the attached drawings). If the specific posture changes, the directional indications will also change accordingly.

[0030] In the present invention, unless otherwise clearly defined and limited, the terms "connection", "fixation", etc. shall be understood in a broad sense. For example, "fixation" can be a fixed connection, a detachable connection, or integrated; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium, and it can be the communication inside two components or the interaction relationship between two components, unless otherwise clearly limited. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0031] In addition, if there are descriptions involving "first", "second", etc. in the embodiments of the present invention, the descriptions of "first", "second", etc. are only for descriptive purposes and cannot be understood as indicating or implying their relative importance or implicitly indicating the quantity of the indicated technical features. Thus, the features defined with "first" and "second" may explicitly or implicitly include at least one such feature. In addition, the meaning of "and / or" appearing throughout the text includes three parallel solutions. Taking "A and / or B" as an example, it includes solution A, solution B, or the solution where A and B are satisfied simultaneously. In addition, the technical solutions between various embodiments can be combined with each other, but it must be based on the fact that those of ordinary skill in the art can implement them. When the combination of technical solutions is contradictory or cannot be implemented, it should be considered that such a combination of technical solutions does not exist and is not within the protection scope required by the present invention.

[0032] Embodiment

[0033] An immobilized enzyme refers to an enzyme that is immobilized in a specific carrier or structure by physical or chemical methods, so that it remains in a fixed position during the reaction process instead of being dissolved in the reaction solution in a free state.

[0034] Reference Figures 1-5, An immobilized enzyme reactor for preparing low-carbon chain triglycerides, comprising: a reactor body 101, the reactor body 101 is provided with a liquid discharge port 102; a spiral filter tube 103 is fixed inside the reactor body 101, and the filter tube 103 is provided with a plurality of flow holes 1031; a vibrator is provided on the outer side wall of the filter tube 103; a plurality of carrier balls 200, the carrier balls 200 are provided with honeycomb-shaped pores, and the inner surface of the pores is attached with an enzyme, and the carrier balls 200 are located inside the filter tube 103; a recovery assembly, including a feed pipe 301, a discharge pipe 302, a conveying channel 303, a conveying mechanism and a collector 305; one end of the feed pipe 301 is communicated with the upper end of the filter tube 103, and the other end of the feed pipe 301 is communicated with the conveying channel 303; one end of the discharge pipe 302 is communicated with the lower end of the filter tube 103, and the other end of the discharge pipe 302 is communicated with the upper part inside the collector 305; the upper end of the collector 305 is provided with a discharging port and a cover plate 3051 for sealing the discharging port, the lower end of the collector 305 is funnel-shaped and communicated with the lower part of the conveying channel 303, and valves are provided on the lower end of the collector 305, the feed pipe 301 and the discharge pipe 302; the diameters of the feed pipe 301, the discharge pipe 302 and the discharging port are all larger than the diameter of the carrier ball 200; the conveying mechanism is located inside the conveying channel 303 and is used for conveying the carrier ball 200 close to the discharging port to the feed pipe 301.

[0035] Before the reactor body 101 is filled with the substrate liquid, the valves of the feed pipe 301 and the discharge pipe 302 are closed to prevent the substrate from flowing out of the reactor body 101. When preparing low-carbon chain triglycerides, the substrate liquid in the reactor body 101 can pass through the flow holes 1031 of the filter tube 103 to contact the carrier balls 200, so that the enzyme on the carrier balls 200 can catalyze the substrate liquid to promote the reaction. The collector 305 contains unused carrier balls 200. When the immobilized enzyme needs to be recovered, the reactor body 101 discharges the substrate liquid from the liquid discharge port 102. At this time, the valves on the lower end of the collector 305, the feed pipe 301 and the discharge pipe 302 are opened, and the vibrator vibrates the filter tube 103. The carrier balls 200 in the filter tube 103 roll into the collector 305 from the discharge pipe 302 under the combined action of vibration and gravity. The unused carrier balls 200 in the collector 305 are moved to the feed pipe 301 through the conveying mechanism and finally enter the filter tube 103; the operator can open the cover plate 3051 of the collector 305 and take out the used carrier balls 200 from the discharging port, thereby achieving the effect of recovering the immobilized enzyme.

[0036] The vibrator may include a vibration motor 104 and a toggle rod 105 connected in transmission. The vibration motor 104 may be arranged on the reactor body 101, and a buffer mechanism, such as sponge and spring, etc., may be arranged between the vibration motor 104 and the reactor body 101. The end of the toggle rod 105 abuts against the outer side wall of the filter tube 103, and sponge or rubber may be arranged between the toggle rod 105 and the filter tube 103. The vibration motor 104 drives the toggle rod 105 to vibrate, and the toggle rod 105 then forces the filter tube 103 to shake, so that the bearing balls 200 in the filter tube 103 can move along the filter tube 103, avoiding the blockage of the bearing balls 200 at the positions with a smaller bending radius of the filter tube 103.

[0037] The bearing ball 200 may be designed with a hollow interior to reduce the mass of the bearing ball 200.

[0038] As an alternative embodiment, it further includes a first liquid storage tank 401, a first pipe 402, a first pump body 403 and an ultrasonic generator 404; the first liquid storage tank 401 contains a first reaction liquid; both ends of the first pipe 402 are respectively communicated with the first liquid storage tank 401 and the collector 305; the first pump body 403 is arranged on the first pipe 402; the ultrasonic generator 404 is arranged on the collector 305; the collector 305 is provided with a drain pipe 3052.

[0039] The first reaction liquid may be n-hexane. After the substrate liquid in the reactor body 101 reacts for a period of time, the enzyme on the bearing ball 200 will be inactivated. At this time, the bearing ball 200 is transported into the collector 305 through the filter tube 103 and the discharge pipe 302. The first reaction liquid is pumped into the collector 305 by the first pump body 403, and the bearing ball 200 is immersed in the first reaction liquid for 1 hour. At this time, the ultrasonic generator 404 is turned on. After removing the grease on the surface of the bearing ball 200 by ultrasonic waves, the drain pipe 3052 can be opened to discharge the first reaction liquid carrying the grease from the collector 305.

[0040] As an alternative embodiment, it further includes a second liquid storage tank 501, a second pipe 502, a second pump body 503, a heater 504, a vibration assembly and a plurality of flexible pipes 506; the second liquid storage tank 501 contains a second reaction liquid; both ends of the second pipe 502 are respectively communicated with the second liquid storage tank 501 and the collector 305; the second pump body 503 is arranged on the second pipe 502; the collector 305 is arranged on the vibration assembly; the discharge pipe 302, the conveying channel 303, the first pipe 402 and the second pipe 502 are respectively communicated with the collector 305 through the flexible pipes 506; the heater 504 is arranged on the collector 305.

[0041] After the grease on the bearing ball 200 is removed by the first reaction liquid, the second reaction liquid is pumped from the second liquid storage tank 501 into the collector 305 by the second pump body 503. The second reaction liquid may contain Ca2+ The buffer solution has a pH value of 7, and then the heater 504 is used to heat the second reaction solution so that the temperature of the second reaction solution is 50 °C. Then, the vibration assembly is used to oscillate the collector 305 for 2 hours to activate the enzyme on the carrier ball 200.

[0042] The heater 504 can be an S-shaped heating tube, and the heater 504 is located on the inner side wall of the collector 305.

[0043] A thermometer for detecting the liquid temperature in the collector 305 can be provided on the collector 305. The operator can control the operation of the heater 504 according to the temperature of the second reaction solution to keep the second reaction solution at about 50 °C.

[0044] The discharge pipe 302, the conveying channel 303, the first pipe 402, and the second pipe 502 are all connected to the collector 305 through the flexible pipe 506, so that the vibration assembly will not drive the pipes to shake when driving the collector 305 to vibrate. The flexible pipe 506 has the function of bending and stretching.

[0045] As an alternative implementation, the vibration assembly includes a base 5051, a mounting plate 5052, a crank-link mechanism 5053, and two pairs of swing rods 5054; the two pairs of swing rods 5054 are respectively located on both sides of the mounting plate 5052, and each pair of swing rods 5054 is distributed in an inverted "V" structure. One end of the swing rod 5054 is rotatably connected to the base 5051, and the other end of the swing rod 5054 is rotatably connected to the mounting plate 5052; the collector 305 is arranged on the mounting plate 5052; the crank-link mechanism 5053 is arranged on the base 5051 and is used to drive the mounting plate 5052 to vibrate.

[0046] A third motor is provided on the base 5051. The third motor is drivingly connected to the crank of the crank-link mechanism 5053, and the connecting rod of the crank-link mechanism 5053 is rotatably connected to the mounting plate 5052. The third motor is located at one end of the base 5051, and the end of the connecting rod away from the third motor is close to the other end of the base 5051. The connecting rod extends in the length direction of the base 5051.

[0047] Each pair of swing rods 5054 is distributed in an inverted "V" structure. When one swing rod 5054 is perpendicular to the base 5051, the other swing rod 5054 will be inclined to the base 5051, causing the mounting plate 5052 to be inclined. When the third motor drives the crank and connecting rod mechanism 5053 to operate, the connecting rod can drive the mounting plate 5052 to reciprocate in the length direction of the base 5051, and the distance between the mounting plate 5052 and the base 5051 changes during the movement, thereby realizing the function of the mounting plate 5052 vibrating relative to the base 5051. The collector 305 is located on the mounting plate 5052 and can vibrate together with the mounting plate 5052, causing the second reaction liquid in the collector 305 to oscillate, so that the second reaction liquid can contact the enzyme on the carrier ball 200 to promote the activation of the enzyme.

[0048] As an alternative embodiment, it further includes a vacuum pump 601 and a third pipe 602; both ends of the third pipe 602 are respectively connected to the vacuum pump 601 and the collector 305.

[0049] When the collector 305 discharges the second reaction liquid through the drain pipe 3052, the collector 305 can close the valves of the discharge pipe 302, the first pipe 402, the second pipe 502 and the drain pipe 3052, use the vacuum pump 601 to evacuate the collector 305 through the third pipe 602, and then turn on the heater 504 in the collector 305 to keep the temperature in the collector 305 at 60°C. After drying the carrier ball 200 for a period of time, turn off the vacuum pump 601 and the heater 504, and conduct a sampling test on the activity of the enzyme on the carrier ball 200. After the enzyme activity meets the standard, the carrier ball 200 can be conveyed to the feed pipe 301 by the conveying mechanism subsequently.

[0050] As an alternative embodiment, the conveying mechanism includes a first motor, a conveyor belt 3041, a driving roller 3042 and a plurality of driven rollers 3043; both the driving roller 3042 and the driven rollers 3043 are rotatably connected to the conveying channel 303, and the conveyor belt 3041 is drivingly connected to the driving roller 3042 and the driven rollers 3043; the first motor is arranged on the conveying channel 303 and is used to drive the driving roller 3042 to rotate; a plurality of stoppers 3044 are arranged along the length direction of the conveyor belt 3041, and the distance between two adjacent stoppers 3044 forms a card slot for clamping the carrier ball 200.

[0051] Let the radius of the carrier ball 200 be R, the distance between the surface of the conveyor belt 3041 and the inner wall of the conveying channel 303 be a, and 2R < d < 3R; the distance between adjacent baffles is b, and 2R < d < 7R / 3; the height of the stopper 3044 (i.e., the distance between the end of the stopper 3044 and the surface of the conveyor belt 3041) is greater than R.

[0052] When the carrier balls 200 in the collector 305 enter the conveying channel 303 from the collector 305, the carrier balls 200 can be stuck into the card slots between the stoppers 3044. The first motor (not shown in the figure) drives the conveyor belt 3041 to move through the driving roller 3042, thereby carrying the carrier balls 200 from small to large to the feed pipe 301. Convex teeth can be provided on the inner surface of the conveyor belt 3041, and grooves matching the convex teeth can be provided on the outer side walls of the driving roller 3042 and the driven roller.

[0053] As an alternative embodiment, a square groove penetrating through the stopper 3044 is provided in the middle of the stopper 3044. The square groove divides the stopper 3044 into two clamping blocks 30441 arranged along the width direction of the conveyor belt 3041. The width of the square groove is greater than the radius of the carrier ball 200; the feed pipe 301 is provided with a receiving groove 3011 matching the clamping block 30441.

[0054] The feed pipe 301 is inserted into the interior of the conveying channel 303. When the conveyor belt 3041 rotates in the conveying channel 303, the stopper 3044 on the conveyor belt 3041 may interfere with the feed pipe 301. Therefore, a square groove is provided in the middle of the stopper 3044 to divide the stopper 3044 into two clamping blocks 30441. The feed pipe 301 is provided with two receiving grooves 3011 matching the clamping blocks 30441. The part between the two receiving grooves 3011 forms a support plate for supporting the carrier balls 200. When the carrier balls 200 enter the feed pipe 301, the carrier balls 200 can roll along the support plate into the inside of the feed pipe 301.

[0055] As an alternative embodiment, it further includes a second motor 106, a reversing transmission mechanism 107 and a stirrer 108; the stirrer 108 is rotatably connected to the reactor body 101, and the stirrer 108 and the filter pipe 103 are concentric; the second motor 106 is arranged on the reactor body 101; the reversing transmission mechanism 107 is used to transmit the power of the second motor 106 to the stirrer 108 and drive the stirrer 108 to rotate forward and backward.

[0056] The filter pipe 103 surrounds the stirrer 108. When the stirrer 108 agitates the substrate liquid to rotate, the substrate liquid can pass through the flow holes 1031 of the filter pipe 103 and contact the carrier balls 200 in the filter pipe 103. The second motor 106 drives the stirrer 108 to rotate forward and backward through the reversing transmission mechanism 107, so that the substrate liquid forms a turbulent flow in the reactor body 101, so that the substrate liquid can fully contact the carrier balls 200.

[0057] The agitator 108 may include a connecting shaft 1081, a plurality of blades 1082 and a plurality of fixing rings 1083; the connecting shaft 1081 is in transmission connection with the second motor 106; every three blades 1082 and one fixing ring 1083 form an agitating blade, and the fixing ring 1083 is sleeved on the outer ring of the three blades 1082 and fixedly connected to the blades 1082, so that when the agitating blade rotates in the substrate liquid, the blades 1082 can withstand a large tangential force.

[0058] As an alternative embodiment, the commutation transmission mechanism 107 includes a gear 1071, a rack 1072 and a driving crank 1073; the reactor body 101 is provided with a connecting plate 109; the driving crank 1073 is rotatably connected to the connecting plate 109, and the driving crank 1073 is provided with a traction shaft 1075; the second motor 106 is arranged on the connecting plate 109 and is used to drive the driving crank 1073 to rotate; the rack 1072 is slidably arranged on the connecting plate 109 and the sliding direction is parallel to the length direction of the rack 1072; the rack 1072 is provided with a mounting block 1074, and the mounting block 1074 is provided with a sliding hole 10741 whose length direction is perpendicular to the length direction of the rack 1072, and the sliding hole 10741 cooperates with the traction shaft 1075; the gear 1071 is rotatably arranged on the connecting plate 109 and is coaxially connected to the agitator 108, and the gear 1071 is in transmission engagement with the rack 1072.

[0059] The second motor 106 drives the driving crank 1073 to rotate, the traction shaft 1075 on the driving crank 1073 drives the rack 1072 to slide back and forth along the connecting plate 109, and the gear 1071 rotates forward and backward along with the movement of the rack 1072, thereby realizing the forward and reverse rotation of the agitator 108 relative to the reactor body 101. The rack 1072 may be provided with a limit block, and the connecting plate 109 may be provided with a limit hole cooperating with the limit block. The limit hole is a strip-shaped hole. When the rack 1072 slides relative to the connecting plate 109, the limit block moves in the limit hole to prevent the rack 1072 from laterally moving.

[0060] The number of the connecting plates 109 may be two. The gear 1071, the rack 1072 and the driving crank 1073 may be located between the two connecting plates 109. The rotating shaft of the agitator 108 passes through the connecting plate 109 and is connected to the gear 1071, and the rotating shaft of the second motor 106 passes through the connecting plate 109 and is connected to the driving crank 1073.

[0061] As an alternative embodiment, a sampling port 3012 and a valve are provided on the feed pipe 301, and the sampling port 3012 is located between the reactor body 101 and the valve.

[0062] The operator needs to regularly inspect the activity of the enzyme on the carrier ball 200. Before the inspection, close the valve on the feed pipe 301 to prevent the substrate liquid in the reactor body 101 from flowing into the conveying channel 303 through the feed pipe 301. Then, take out the carrier ball 200 in the filter pipe 103 and close to the feed pipe 301 from the sampling port 3012, and then inspect the enzyme on the carrier ball 200.

[0063] The valves on the lower end of the collector 305, the feed pipe 301, and the discharge pipe 302 can all be full-port ball valves.

[0064] The above are only alternative embodiments of the present invention, and do not limit the patent scope of the present invention. Any equivalent structural transformation made under the inventive concept of the present invention by using the content of the specification and drawings of the present invention, or directly / indirectly applied in other related technical fields, is included in the patent protection scope of the present invention.

Claims

1. An immobilized enzyme reactor for preparing low-carbon chain triglycerides, characterized in that, Comprising: A reactor body, the reactor body being provided with a liquid discharge port; a spiral filter tube is fixed inside the reactor body, the filter tube being provided with a plurality of flow holes; a vibrator is provided on the outer side wall of the filter tube; A plurality of carrier balls, the carrier balls being provided with honeycomb-shaped pores, the inner surface of the pores being attached with enzymes, the carrier balls being located inside the filter tube; A recovery assembly, including a feed pipe, a discharge pipe, a conveying channel, a conveying mechanism and a collector; one end of the feed pipe is communicated with the upper end of the filter tube, and the other end of the feed pipe is communicated with the conveying channel; one end of the discharge pipe is communicated with the lower end of the filter tube, and the other end of the discharge pipe is communicated with the upper part inside the collector; a discharge port and a cover plate for sealing the discharge port are provided at the upper end of the collector, the lower end of the collector is in a funnel shape and is communicated with the lower part of the conveying channel, valves are provided at the lower end of the collector, the feed pipe and the discharge pipe; the diameters of the feed pipe, the discharge pipe and the discharge port are all larger than the diameter of the carrier ball; the conveying mechanism is located inside the conveying channel and is used for conveying the carrier ball close to the discharge port to the feed pipe.

2. The immobilized enzyme reactor for preparing triglycerides with low carbon chains as claimed in claim 1, wherein, It further includes a first liquid storage tank, a first pipe, a first pump body and an ultrasonic generator; the first liquid storage tank is internally filled with a first reaction liquid; both ends of the first pipe are respectively communicated with the first liquid storage tank and the collector; the first pump body is arranged on the first pipe; the ultrasonic generator is arranged on the collector; the collector is provided with a liquid discharge pipe.

3. The immobilized enzyme reactor for preparing low-carbon-chain triglycerides according to claim 2, wherein It further includes a second liquid storage tank, a second pipe, a second pump body, a heater, a vibration assembly and a plurality of flexible pipes; the second liquid storage tank is internally filled with a second reaction liquid; both ends of the second pipe are respectively communicated with the second liquid storage tank and the collector; the second pump body is arranged on the second pipe; the collector is arranged on the vibration assembly; the discharge pipe, the conveying channel, the first pipe and the second pipe are respectively communicated with the collector through the flexible pipes; the heater is arranged on the collector.

4. The immobilized enzyme reactor for preparing triglycerides with low carbon chains according to claim 3, characterized in that, The vibration assembly includes a base, a mounting plate, a crank and connecting rod mechanism and two pairs of swing rods; the two pairs of swing rods are respectively located on both sides of the mounting plate, each pair of swing rods is distributed in an inverted "eight" character structure, one end of the swing rod is rotatably connected to the base, and the other end of the swing rod is rotatably connected to the mounting plate; the collector is arranged on the mounting plate; the crank and connecting rod mechanism is arranged on the base and is used for driving the mounting plate to vibrate.

5. The immobilized enzyme reactor for preparing low-carbon-chain triglycerides according to claim 3, characterized in that, It further includes a vacuum pump and a third pipe; both ends of the third pipe are respectively communicated with the vacuum pump and the collector.

6. The immobilized enzyme reactor for preparing triglycerides with low carbon chains as claimed in claim 1, wherein, The conveying mechanism includes a first motor, a conveyor belt, a driving roller and a plurality of driven rollers; the driving roller and the driven rollers are both rotatably connected to the conveying channel, the conveyor belt is drivingly connected to the driving roller and the driven rollers; the first motor is arranged on the conveying channel and is used for driving the driving roller to rotate; a plurality of stoppers are arranged along the length direction of the conveyor belt, and the distance between two adjacent stoppers forms a card slot for clamping the carrier ball.

7. The immobilized enzyme reactor for preparing low-carbon chain triglycerides according to claim 6, characterized in that, A square groove penetrating the middle of the stopper is provided, and the square groove divides the stopper into two clamping blocks arranged along the width direction of the conveyor belt. The width of the square groove is greater than the radius of the bearing ball; the feed pipe is provided with a receiving groove that cooperates with the clamping blocks.

8. The immobilized enzyme reactor for preparing low-carbon chain triglycerides according to claim 1, characterized in that, It further includes a second motor, a reversing transmission mechanism, and a stirrer; the stirrer is rotatably connected to the reactor body, and the stirrer and the filter pipe share the same central axis; the second motor is arranged on the reactor body; the reversing transmission mechanism is used to transmit the power of the second motor to the stirrer and drive the stirrer to rotate forward and backward.

9. The immobilized enzyme reactor for preparing low-carbon chain triglycerides according to claim 8, characterized in that, The reversing transmission mechanism includes a gear, a rack, and a driving crank; the reactor body is provided with a connecting plate; the driving crank is rotatably connected to the connecting plate, and the driving crank is provided with a traction shaft; the second motor is arranged on the connecting plate and is used to drive the driving crank to rotate; the rack is slidably arranged on the connecting plate and the sliding direction is parallel to the length direction of the rack; the rack is provided with a mounting block, and the mounting block is provided with a sliding hole whose length direction is perpendicular to the length direction of the rack, and the sliding hole cooperates with the traction shaft; the gear is rotatably arranged on the connecting plate and is coaxially connected to the stirrer, and the gear is in transmission engagement with the rack.

10. The immobilized enzyme reactor for preparing low-carbon-chain triglycerides according to claim 1, wherein A sampling port and a valve are arranged on the feed pipe, and the sampling port is located between the reactor body and the valve.

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