Rapid determination sampler for food allergens based on quantum dot labeling
By designing the cylinder separation structure and an automated sampling and mixing system in food allergen detection, the problem of quantum dot solution being susceptible to environmental influences and inaccurate control of mixing timing is solved, and efficient and accurate detection results are achieved.
Patent Information
- Application Number
- CN202510391774.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-31
- Publication Date
- 2025-07-11
AI Technical Summary
In the prior art, quantum dot solutions are susceptible to environmental factors in the detection of food allergens, and their stability is reduced, and the mixing timing is inaccurate, resulting in inaccurate detection results and poor reliability.
A rapid measurement sampler based on quantum dot marking is designed, using a cylinder to separate it into upper and lower chambers, and the blanking timing of the quantum dot solution is accurately controlled through the structure of the sealing block and the stirring rack. Combined with the coordinated operation of the motor, threaded rod and piston rod, the extraction and mixing of the food solution is automatically completed to ensure that the mixing is carried out in the optimal reaction state.
It improves the accuracy and reliability of the detection, reduces artificial errors, simplifies the detection process, and improves the detection efficiency and stability of the results.
Smart Images
Figure CN120293606A_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to the technical field of food sampling and detection, and particularly to a rapid determination sampler for food allergens based on quantum dot labeling. Background Art
[0002] In the field of food allergen detection, ensuring the accuracy and reliability of detection is of crucial importance. With the continuous increase in people's attention to food safety and the number of food allergy patients, precise detection of food allergens has become a key research direction. Currently, quantum dot labeling technology has gradually been applied in food allergen detection due to its unique advantages. However, there are many problems in the solution mixing and storage processes during actual operation.
[0003] In traditional detection devices, the treatment methods for quantum dot solutions and food solutions are relatively simple and crude. Usually, there is a lack of effective control over the storage environment of quantum dot solutions. Quantum dot solutions often come into contact with other reagents or the external environment, and are easily affected by factors such as temperature, humidity, light, and impurities, resulting in a decrease in their stability and further affecting the accuracy of detection results. For example, in some detection devices, quantum dot solutions and other reagents are placed in the same container or adjacent spaces, and the quantum dot solutions are easily interfered by the volatile components of other reagents, causing changes in their fluorescence characteristics and reducing the detection sensitivity.
[0004] In terms of solution mixing, the prior art cannot precisely control the mixing timing. Often, after the food sample is collected, the quantum dot solution is prematurely mixed with it. At this time, the food sample may not have been fully processed or the internal allergens have not been completely released, and the reaction has already started with the quantum dot solution, resulting in an incomplete reaction, an incomplete or unstable antigen-antibody-quantum dot complex, and affecting the reliability of the detection results. Additionally, although some detection devices are aware of the importance of the mixing timing, they lack effective control means and cannot mix the quantum dot solution with the food solution in a timely and accurate manner when the food solution reaches the optimal reaction state. Summary of the Invention
[0005] In order to overcome the above-mentioned drawbacks, the present invention provides a rapid determination sampler for food allergens based on quantum dot labeling.
[0006] The technical solution is as follows: A rapid determination sampler for food allergens based on quantum dot labeling, comprising a cylinder body, a connection port, a sampling needle, a sampling cylinder, a threaded rod, a piston rod and a detector. The bottom of the cylinder body is connected with the connection port, and the sampling needle is clamped at the connection port. The sampling cylinder is movably connected inside the cylinder body. The lower end of the sampling cylinder is hermetically communicated with the connection port. The piston rod is slidably connected inside the sampling cylinder through a vertical chute. The top of the piston rod is rotatably connected with the threaded rod. The threaded rod penetrates through the sampling cylinder. The detector is arranged at a position below and to the right of the cylinder body. It further comprises a partition plate, a stirring frame, a sealing block and a connecting rod. The upper side inside the cylinder body is connected with the partition plate, which divides the cylinder body into upper and lower chambers. The stirring frame is rotatably connected at the lower chamber inside the cylinder body. The stirring frame is in spline connection with the sampling cylinder. The connecting rod is slidably connected to the right side of the cylinder body. The bottom of the connecting rod is connected with the sealing block. A notch is opened on the right side of the partition plate, and a notch is also opened on the right side of the top of the stirring frame. The sealing block is in plug-in fit with the notch.
[0007] Further, a special rubber sealing ring is used at the connection between the connection port and the sampling needle.
[0008] Further, it also comprises a support frame, a container, a lifting frame and a sliding frame. The support frame stands on the ground, and the lifting frame is vertically slidably connected to the upper side thereof. The sliding frame is slidably connected in the left-right direction on the lifting frame. The cylinder body is installed on the sliding frame. A container for storing the processed food sample solution is placed at a position below the cylinder body on the support frame.
[0009] Further, it also comprises an electric cylinder, a driving module and a conveyor belt. The electric cylinder is installed in the middle of the top of the support frame. The telescopic rod of the electric cylinder is connected to the lifting frame. The driving module is installed on the right side of the lifting frame. The conveyor belt is connected to the rear side of the lifting frame. The sliding frame is connected to the lower half of the conveyor belt by a belt. The output shaft of the driving module is connected to the driving wheel of the conveyor belt.
[0010] Further, it also comprises a limit frame, a rotating gear, a return spring, a lifting disc, a motor, a transmission gear, a compression spring and a limit rod. The limit frame is connected to the top of the cylinder body. The sampling cylinder penetrates through the limit frame, and the threaded rod also penetrates through the limit frame. The limit rod is connected to the front side of the top of the limit frame. The threaded rod is slidably connected to the limit rod. The rotating gear is rotatably connected to the middle of the inner top of the limit frame. The rotating gear is in threaded connection with the threaded rod. The lifting disc is slidably connected to the upper side of the limit frame. Four return springs are connected at intervals between the lifting disc and the limit frame. The sampling cylinder abuts against the bottom surface of the lifting disc. The motor is installed on the right side of the top of the limit frame. The output shaft of the motor penetrates through the limit frame and is connected with the transmission gear. The transmission gear meshes with the rotating gear. A compression spring is connected between the connecting rod and the cylinder body. The lifting disc abuts against the top surface of the connecting rod, so that the compression spring is in a compressed state initially.
[0011] Further, it also comprises a spur gear and a rack. The spur gear is connected to the outer side of the upper end of the sampling cylinder. The rack is connected to the front side of the lifting frame. The rack meshes with the spur gear.
[0012] Furthermore, it further includes a push rod, a support rod, a baffle, and a return spring. A support rod is connected to the right side of the connection port. A baffle is slidably connected to the support rod. The baffle is slidably connected to the connection port and is located on the top surface of the sampling needle. A return spring is connected between the baffle and the connection port. The return spring is sleeved on the support rod. A blanking groove is formed on the right side of the sampling needle. The baffle initially blocks the blanking groove. A through hole is formed in the middle of the baffle. A push rod is connected to the lower right side of the support frame at a position on the right side of the baffle. The push rod is in contact and cooperation with the baffle.
[0013] Furthermore, it further includes a convex block and a toothed ring. A plurality of convex blocks are circumferentially and spacedly connected to the inner bottom of the limit frame. A toothed ring that is clamped and cooperated with the convex blocks is connected to the outer side of the upper end of the sampling cylinder.
[0014] The beneficial effects are as follows: 1. The cylinder body is separated into upper and lower chambers by a partition board, providing an independent and stable storage environment for the quantum dot solution. With the unique plug-in structure of the sealing block with the partition board and the notch of the stirring frame, the blanking timing of the quantum dot solution can be accurately controlled. After the food solution is transferred to the lower chamber of the cylinder body through the sampling cylinder, the blanking channel is opened, ensuring that the two are mixed at the best stage, effectively improving the accuracy and reliability of the detection.
[0015] 2. The motor, threaded rod, piston rod, and electric cylinder cooperate to automatically complete the extraction and transportation of the food solution. The motor drives the threaded rod to precisely control the up and down movement of the piston rod, evacuating the air in the sampling cylinder and extracting the solution. The electric cylinder accurately adjusts the height of the cylinder body, enabling the sampling needle to accurately insert into the sample container, greatly improving the sampling efficiency and reducing human error.
[0016] 3. When moving horizontally, through the cooperation of the spur gear and the rack, the sampling cylinder and the stirring frame are driven to rotate, fully mixing the food sample solution and the quantum dot solution, improving the accuracy of the detection result, and thus improving the working efficiency of the entire device.
[0017] 4. An automatic blanking system is composed of a push rod, a support rod, a baffle, and a return spring. When the cylinder body moves to the right, the baffle moves left after contacting the push rod, aligning the through hole with the blanking groove, automatically discharging the mixed solution to the detection port of the detector. After the detection is completed, the baffle automatically resets, simplifying the detection process and improving the detection efficiency. Description of the Drawings
[0018] Figure 1 It is a three-dimensional structural schematic diagram of the present invention.
[0019] Figure 2 It is a three-dimensional structural schematic diagram of the cylinder body, sampling cylinder, threaded rod and other components of the present invention.
[0020] Figure 3 It is a three-dimensional structural schematic diagram of the stirring frame, sampling cylinder, sampling needle and other components of the present invention.
[0021] Figure 4 This is a schematic plan view of components such as the stirring frame, sealing block and connecting rod of the present invention.
[0022] Figure 5 This is a schematic three-dimensional view of components such as the motor, transmission gear and rotating gear of the present invention.
[0023] Figure 6 This is a schematic three-dimensional view of components such as the lifting disc, sealing block and connecting rod of the present invention.
[0024] Figure 7 This is a schematic plan view of components such as the stirring frame, partition plate and sealing block of the present invention.
[0025] Figure 8 This is a schematic three-dimensional view of components such as the lifting frame, spur gear and rack of the present invention.
[0026] Figure 9 This is a schematic plan view of components such as the push rod, baffle and connecting port of the present invention.
[0027] Figure 10 This is a schematic three-dimensional view of components such as the support rod, baffle and return spring of the present invention.
[0028] Figure 11 This is a schematic three-dimensional view of components such as the return spring, blanking chute and sampling needle of the present invention.
[0029] Figure 12 This is an exploded view of components such as the convex block, toothed ring and support frame of the present invention.
[0030] Names and serial numbers of components in the figure: 1 - cylinder body, 101 - connecting port, 102 - sampling needle, 103 - sampling cylinder, 104 - threaded rod, 105 - piston rod, 106 - partition plate, 107 - detector, 108 - stirring frame, 109 - sealing block, 1010 - connecting rod, 201 - support frame, 202 - container, 203 - lifting frame, 204 - sliding frame, 205 - electric cylinder, 206 - driving module, 207 - conveyor belt, 301 - limiting frame, 302 - rotating gear, 303 - return spring, 304 - lifting disc, 305 - motor, 306 - transmission gear, 307 - compression spring, 308 - limiting rod, 401 - spur gear, 402 - rack, 501 - push rod, 502 - support rod, 503 - baffle, 504 - return spring, 505 - through hole, 506 - blanking chute, 601 - convex block, 602 - toothed ring. Detailed implementation manners
[0031] The technical solution of the present invention will be further described below with reference to the accompanying drawings.
[0032] Example: A rapid determination sampler for food allergens based on quantum dot labeling, asFigures 1 - 7 As shown in the figure, it includes a cylinder body 1, a connection port 101, a sampling needle 102, a sampling cylinder 103, a threaded rod 104, a piston rod 105, a partition plate 106, a detector 107, a stirring frame 108, a sealing block 109 and a connecting rod 1010. The bottom of the cylinder body 1 is connected with the connection port 101. The sampling needle 102 is clamped at the connection port 101, and the sampling needle 102 is a one-way structure, which can ensure that the liquid can only enter from the outside and prevent the sample from flowing back. A special rubber sealing ring is used at the connection between the connection port 101 and the sampling needle 102, and its material has the characteristics of acid and alkali resistance and organic solvent resistance, which can effectively prevent the solution from leaking, ensuring that during the whole detection process, the sample solution and the quantum dot solution are strictly sealed in the device, avoiding interference from the external environment and volatilization loss of the solution, and improving the stability of the detection result. The sampling cylinder 103 is movably connected in the cylinder body 1. The lower end of the sampling cylinder 103 is hermetically communicated with the connection port 101, ensuring the tightness during the sampling process of the sample. The piston rod 105 is slidably connected in the sampling cylinder 103 through a vertical chute, and the piston rod 105 can only slide up and down along the sampling cylinder 103 and will not rotate in the sampling cylinder 103. The top of the piston rod 105 is rotatably connected with the threaded rod 104, and the threaded rod 104 penetrates through the sampling cylinder 103. The upper side in the cylinder body 1 is connected with the partition plate 106, which divides the cylinder body 1 into upper and lower chambers. The upper chamber is specially used for storing the quantum dot solution, and the lower chamber is used as the mixing chamber for the food sample solution and the quantum dot solution. The detector 107 is arranged at a position on the lower right side of the cylinder body 1, and its function is to detect the processed quantum dot complex, and determine the presence and content of the allergen by detecting the fluorescence signal of the quantum dot. The stirring frame 108 is rotatably connected at the lower chamber in the cylinder body 1. The stirring frame 108 and the sampling cylinder 103 are in spline connection, which not only allows the sampling cylinder 103 to slide up and down, but also can drive the stirring frame 108 to rotate synchronously when the sampling cylinder 103 rotates, so as to realize the stirring function of the mixed solution. The connecting rod 1010 is slidably connected to the right side of the cylinder body 1. The bottom of the connecting rod 1010 is connected with the sealing block 109. A notch is opened on the right side of the partition plate 106, and a notch is also opened on the right side of the top of the stirring frame 108. The sealing block 109 is inserted and matched with the notch to control the opening and closing of the notch, and further control the flow path of the quantum dot solution.
[0033] As Figures 1 - 3As shown in the figure, it further includes a support frame 201, a container 202, a lifting frame 203, a sliding frame 204, an electric cylinder 205, a driving module 206 and a conveyor belt 207. The support frame 201 stands on the ground and provides stable support for the entire device. A lifting frame 203 is vertically slidably connected to the upper side thereof. A sliding frame 204 is slidably connected in the left-right direction on the lifting frame 203. The cylinder body 1 is installed on the sliding frame 204, enabling the cylinder body 1 to move flexibly in two directions. A container 202 for storing the processed food sample solution is placed at a position below the cylinder body 1 on the support frame 201. An electric cylinder 205 is installed in the middle of the top of the support frame 201 through bolts. The telescopic rod of the electric cylinder 205 is connected to the lifting frame 203. By the telescoping of the electric cylinder 205, the vertical position of the lifting frame 203 can be precisely controlled, thereby realizing the up and down movement of the cylinder body 1, facilitating the sampling needle 102 to insert into the container 202. A driving module 206 is installed on the right side of the lifting frame 203 through bolts. A conveyor belt 207 is connected to the rear side of the lifting frame 203. The sliding frame 204 is connected to the lower half of the conveyor belt 207 by a belt. The output shaft of the driving module 206 is connected to the driving wheel of the conveyor belt 207. By driving the conveyor belt 207 to rotate through the driving module 206, the sliding frame 204 can be driven to move left and right along the lifting frame 203, and further realize the position adjustment of the cylinder body 1 in the horizontal direction.
[0034] As Figures 3 - 8As shown in the figure, it further includes a limit frame 301, a rotating gear 302, a return spring 303, a lifting disc 304, a motor 305, a transmission gear 306, a compression spring 307, a limit rod 308, a gear 401 and a rack 402. The top of the cylinder body 1 is connected with the limit frame 301. The sampling cylinder 103 passes through the limit frame 301, and the threaded rod 104 also passes through the limit frame 301. The front side of the top of the limit frame 301 is connected with the limit rod 308. The threaded rod 104 is slidably connected with the limit rod 308, and it can only slide up and down under the limitation of the limit rod 308 and cannot rotate. The middle of the inner top of the limit frame 301 is rotatably connected with the rotating gear 302. The rotating gear 302 is threadedly connected with the threaded rod 104. By rotating the rotating gear 302, the up and down movement of the threaded rod 104 can be realized. The lifting disc 304 is slidably connected to the upper side of the limit frame 301. Four return springs 303 are connected at intervals between the lifting disc 304 and the limit frame 301. The sampling cylinder 103 abuts against the bottom surface of the lifting disc 304. The motor 305 is installed on the right side of the top of the limit frame 301 through bolts. The output shaft of the motor 305 passes through the limit frame 301 and is connected with the transmission gear 306. The transmission gear 306 meshes with the rotating gear 302. A compression spring 307 is connected between the connecting rod 1010 and the cylinder body 1. The lifting disc 304 abuts against the top surface of the connecting rod 1010, so that the compression spring 307 is in a compressed state initially, and the sealing block 109 is in a state of blocking the notch of the stirring frame 108. The outer side of the upper end of the sampling cylinder 103 is connected with a spur gear 401. The front side of the lifting frame 203 is connected with a rack 402. The rack 402 meshes with the spur gear 401.
[0035] When measuring food allergens, first, according to the relevant principles of biochemistry and material processing, the ground food sample is mixed with an appropriate amount of buffer solution to form a homogenate. From the perspective of the biological molecule release mechanism, the buffer solution can maintain an appropriate pH environment, which helps to destroy the cell structure of the food sample and release the allergens therein into the buffer solution sufficiently. After the homogenate preparation is completed, the mixed food solution is added into the container 202. At the same time, a solution containing quantum dot-labeled specific antibodies is prepared and injected into the upper chamber of the cylinder body 1. At this time, in the initial state, the sealing block 109 blocks the notch of the stirring frame 108, while the notch on the partition plate 106 is in an open state, so that the solution in the upper chamber can flow into the chamber between the partition plate 106 and the stirring frame 108 under the action of gravity and fluid pressure difference.
[0036] After all the preparatory work is completed, start the motor 305. The rotation of the output shaft of the motor 305 drives the transmission gear 306 to rotate, and then drives the rotating gear 302 to rotate. Since the rotating gear 302 is threadedly connected to the threaded rod 104, and at the same time the threaded rod 104 is limited by the limiting rod 308, the rotation of the rotating gear 302 will drive the threaded rod 104 and the piston rod 105 to move downward, exhausting the air inside the sampling cylinder 103 to form a negative pressure. After the piston rod 105 moves downward to the limit, turn off the motor 305. Then start the electric cylinder 205. The extension of the telescopic rod of the electric cylinder 205 drives the lifting frame 203 to move downward, thereby driving the sliding frame 204 and the cylinder body 1 to move downward as a whole, so that the sampling needle 102 enters the food solution in the container 202. Start the motor 305 again. The motor 305 drives the transmission gear 306 and the rotating gear 302 to rotate in the reverse direction, driving the threaded rod 104 and the piston rod 105 to move upward. At this time, the negative pressure state in the sampling cylinder 103 and the suction force formed by the upward movement of the piston rod 105, based on the principle of fluid mechanics that the pressure difference causes fluid flow, will pump the food solution in the container 202 into the sampling cylinder 103 through the sampling needle 102. After the extraction is completed, reverse the operation of the electric cylinder 205 to drive the lifting frame 203, the sliding frame 204 and the cylinder body 1 to move upward as a whole back to the initial height. During this process, the motor 305 continues to operate. When the piston rod 105 moves upward to abut against the top of the sampling cylinder 103, the rotating gear 302 continues to rotate, driving the threaded rod 104 and the piston rod 105 to continue to move upward. The piston rod 105 drives the sampling cylinder 103 to move upward along the cylinder body 1. As the sampling cylinder 103 moves upward, its inner cavity will communicate with the lower cavity of the cylinder body 1. Based on the principle of fluid communication, at this time, the food solution in the sampling cylinder 103 will fall into the lower cavity of the cylinder body 1 under the action of gravity. The upward movement of the sampling cylinder 103 will push the lifting plate 304 to move upward along the limiting frame 301, and the return spring 303 is further compressed. Since the lifting plate 304 blocked the connecting rod 1010 initially, when the lifting plate 304 moves upward, the compression spring 307 will rebound and reset according to the principle of conversion of elastic potential energy into kinetic energy, driving the connecting rod 1010 and the sealing block 109 to move upward. The sealing block 109 no longer blocks the notch on the stirring frame 108. At this time, based on the principle of fluid pressure difference and communication, the quantum dot solution in the chamber between the partition plate 106 and the stirring frame 108 will flow into the lower chamber of the cylinder body 1 to contact the food solution. At the same time, the upward movement of the sealing block 109 will block the notch on the partition plate 106, so that the quantum dot solution in the upper chamber of the cylinder body 1 will no longer flow into the chamber between the partition plate 106 and the stirring frame 108. This process effectively controls the flow direction of the solution and ensures the orderly progress of the reaction. The spur gear 401 moves upward with the sampling cylinder 103. After the spur gear 401 moves to mesh with the rack 402 on the front side of the lifting frame 203, turn off the motor 305.
[0037] Next, start the drive module 206. The drive module 206 drives the conveyor belt 207 to rotate, so as to drive the sliding frame 204 to move to the right along the lifting frame 203, and then drive the entire cylinder body 1 to move to the right. During the movement, the spur gear 401 meshes with the rack 402, and the spur gear 401 will rotate on its own axis, and then drive the sampling cylinder 103 to rotate. The sampling cylinder 103 and the stirring frame 108 are connected by splines. According to the transmission principle of spline connection, the rotation of the sampling cylinder 103 will drive the stirring frame 108 to rotate, so as to fully mix the quantum dot solution and the food solution in the lower chamber. After the sliding frame 204 moves to the right to the limit, the cylinder body 1 is aligned with the detector 107. At this time, the quantum dot solution and the food solution have been mixed into a quantum dot complex.
[0038] At this time, the sampling needle 102 can be removed, so that the complex in the lower chamber of the cylinder body 1 is discharged onto the detection port of the detector 107. The detector 107 detects the complex through the detection port according to the quantum dot fluorescence detection principle, and determines the type and content of the allergen based on the detected quantum dot fluorescence signal. After different types and contents of allergens bind to the specific antibodies labeled with quantum dots, characteristic changes will occur in aspects such as the intensity and wavelength of the quantum dot fluorescence signal. By analyzing these changes, the relevant information of the allergen can be determined. After the detection is completed, perform a reset operation on the device. First, start the motor 305 to drive the threaded rod 104 and the piston rod 105 to move downward. The sampling cylinder 103 is no longer subjected to the upward thrust of the piston rod 105, and the sampling cylinder 103 and the lifting disk 304 will be in a relaxed state. At this time, the reset spring 303 rebounds and resets, that is, it drives the lifting disk 304 to move downward. The lifting disk 304 then pushes the sampling cylinder 103 to move downward and reset. The downward movement of the lifting disk 304 synchronously squeezes the connecting rod 1010 and the sealing block 109 to move downward and reset. The sealing block 109 disengages from the notch on the partition plate 106, and then blocks the notch on the stirring frame 108. In this way, the solution in the upper chamber will enter the chamber between the partition plate 106 and the stirring frame 108 again based on the principle of fluid communication, waiting for the next use. After the sampling cylinder 103 is reset, control the drive module 206 to rotate in the reverse direction, drive the conveyor belt 207 to reverse, so as to drive the sliding frame 204 to move to the left along the lifting frame 203 to reset, and drive the entire cylinder body 1 structure to move to the left to reset. After cleaning the relevant components, according to the above operations, the determination of the allergen of the next type of food can be continued.
[0039] Such as Figures 9 - 11As shown, it also includes a push rod 501, a support rod 502, a baffle 503 and a return spring 504. The support rod 502 is welded on the right side of the connecting port 101. The baffle 503 is slidably connected to the support rod 502. The baffle 503 is slidably connected to the connecting port 101 and is located on the top surface of the sampling needle 102. A return spring 504 is connected between the baffle 503 and the connecting port 101. The return spring 504 is sleeved on the support rod 502. A feeding trough 506 is provided on the right side of the needle 102, and the baffle 503 initially blocks the feeding trough 506. A through hole 505 is provided in the middle of the baffle 503. When the through hole 505 is aligned with the feeding trough 506, the quantum dot complex in the lower chamber of the cylinder 1 can be discharged through the through hole 505 and the feeding trough 506. A push rod 501 is connected to the right side of the lower right side of the support frame 201, which is located on the right side of the baffle 503, and the push rod 501 is in contact with the baffle 503.
[0040] When the sliding frame 204 drives the cylinder 1 to move to the right, the stirring frame 108 mixes the food sample solution and the quantum dot solution, and the baffle 503 moves synchronously. When the baffle 503 contacts the push rod 501, the push rod 501 resists the baffle 503, and the cylinder 1 continues to move, which will force the baffle 503 to move to the left along the support rod 502, and the return spring 504 will be compressed accordingly. At this time, the through hole 505 on the baffle 503 will be aligned with the drop groove 506, and the mixed quantum dot complex in the lower chamber of the cylinder 1 will be discharged downward through the drop groove 506 to the detection port of the detector 107, realizing automatic drop. After the drop is completed, the sliding frame 204 drives the cylinder 1 to move to the left and reset, so that the baffle 503 is disengaged from the push rod 501, the return spring 504 rebounds and resets, and the baffle 503 also moves to the right and resets, thereby blocking the drop groove 506 again.
[0041] like Figure 12 As shown, it also includes a protrusion 601 and a toothed ring 602. A plurality of protrusions 601 are welded at intervals along the circumferential direction on the inner bottom of the limit frame 301. A toothed ring 602 is connected to the outer side of the upper end of the sampling tube 103 and is engaged with the protrusion 601. When the sampling tube 103 is in use, the engagement of the protrusion 601 and the toothed ring 602 can accurately determine that the sampling tube 103 has moved downward to the limit, thereby playing a positioning role. When the sampling tube 103 is not in use and the threaded rod 104 rotates alone, the sampling tube 103 and the piston rod 105 can be prevented from rotating.
[0042] The technical principles of the embodiments of the present invention are described above in conjunction with specific embodiments. These descriptions are only for explaining the principles of the embodiments of the present invention and cannot be interpreted in any way as limiting the protection scope of the embodiments of the present invention. Based on the explanations herein, those skilled in the art can think of other specific implementation methods of the embodiments of the present invention without creative work, and these methods will fall within the protection scope of the embodiments of the present invention.
Claims
1. A rapid determination sampler for food allergens based on quantum dot labeling, comprising a cylinder body (1), a connection port (101), a sampling needle (102), a sampling cylinder (103), a threaded rod (104), a piston rod (105) and a detector (107). A connection port (101) is connected to the bottom of the cylinder body (1), and a sampling needle (102) is clamped at the connection port (101). A sampling cylinder (103) is movably connected inside the cylinder body (1). The lower end of the sampling cylinder (103) is hermetically communicated with the connection port (101). A piston rod (105) is slidably connected inside the sampling cylinder (103) through a vertical chute. The top of the piston rod (105) is rotatably connected to a threaded rod (104). The threaded rod (104) penetrates through the sampling cylinder (103). A detector (107) is arranged at a position below and to the right of the cylinder body (1). It is characterized in that, It further includes a partition plate (106), a stirring frame (108), a sealing block (109) and a connecting rod (1010). A partition plate (106) is connected to the upper side inside the cylinder body (1), dividing the cylinder body (1) into upper and lower chambers. A stirring frame (108) is rotatably connected to the lower chamber inside the cylinder body (1). The stirring frame (108) and the sampling cylinder (103) are in spline connection. A connecting rod (1010) is slidably connected to the right side of the cylinder body (1). The bottom of the connecting rod (1010) is connected to a sealing block (109). A notch is formed on the right side of the partition plate (106), and a notch is also formed on the right side of the top of the stirring frame (108). The sealing block (109) is inserted and matched with the notch.
2. The rapid determination sampler for food allergens based on quantum dot labeling according to claim 1, wherein A special rubber sealing ring is used at the connection between the connection port (101) and the sampling needle (102).
3. A rapid determination sampler for food allergens based on quantum dot labeling according to claim 2, characterized in that, It further includes a support frame (201), a container (202), a lifting frame (203) and a sliding frame (204). The support frame (201) stands on the ground, and a lifting frame (203) is vertically slidably connected to the upper side thereof. A sliding frame (204) is slidably connected in the left-right direction on the lifting frame (203). The cylinder body (1) is installed on the sliding frame (204). A container (202) for storing the processed food sample solution is placed at a position below the cylinder body (1) on the support frame (201).
4. A rapid determination sampler for food allergens based on quantum dot labeling according to claim 3, characterized in that, It further includes an electric cylinder (205), a driving module (206) and a conveyor belt (207). An electric cylinder (205) is installed in the middle of the top of the support frame (201). The telescopic rod of the electric cylinder (205) is connected to the lifting frame (203). A driving module (206) is installed on the right side of the lifting frame (203). A conveyor belt (207) is connected to the rear side of the lifting frame (203). The sliding frame (204) is connected to the lower half of the conveyor belt (207) by a belt. The output shaft of the driving module (206) is connected to the driving wheel of the conveyor belt (207).
5. A rapid determination sampler for food allergens based on quantum dot labeling according to claim 4, characterized in that, It further includes a limit frame (301), a rotating gear (302), a return spring (303), a lifting disc (304), a motor (305), a transmission gear (306), a compression spring (307) and a limit rod (308). A limit frame (301) is connected to the top of the cylinder body (1). The sampling cylinder (103) penetrates through the limit frame (301), and the threaded rod (104) also penetrates through the limit frame (301). A limit rod (308) is connected to the front side of the top of the limit frame (301). The threaded rod (104) is slidably connected to the limit rod (308). A rotating gear (302) is rotatably connected to the middle of the inner top of the limit frame (301). The rotating gear (302) is threadedly connected to the threaded rod (104). A lifting disc (304) is slidably connected to the upper side of the limit frame (301). Four return springs (303) are connected at intervals between the lifting disc (304) and the limit frame (301). The sampling cylinder (103) abuts against the bottom surface of the lifting disc (304). A motor (305) is installed on the right side of the top of the limit frame (301). The output shaft of the motor (305) penetrates through the limit frame (301) and is connected with a transmission gear (306). The transmission gear (306) meshes with the rotating gear (302). A compression spring (307) is connected between the connecting rod (1010) and the cylinder body (1). The lifting disc (304) abuts against the top surface of the connecting rod (1010), so that the compression spring (307) is in a compressed state initially.
6. The rapid determination sampler for food allergens based on quantum dot labeling according to claim 5, wherein, It further includes a spur gear (401) and a rack (402). A spur gear (401) is connected to the outer side of the upper end of the sampling cylinder (103). A rack (402) is connected to the front side of the lifting frame (203). The rack (402) meshes with the spur gear (401).
7. A rapid determination sampler for food allergens based on quantum dot labeling according to claim 6, characterized in that It further includes a push rod (501), a support rod (502), a baffle (503) and a return spring (504). A support rod (502) is connected to the right side of the connection port (101). A baffle (503) is slidably connected to the support rod (502). The baffle (503) is slidably connected to the connection port (101) and is located on the top surface of the sampling needle (102). A return spring (504) is connected between the baffle (503) and the connection port (101). The return spring (504) is sleeved on the support rod (502). A blanking groove (506) is formed on the right side of the sampling needle (102). The baffle (503) blocks the blanking groove (506) initially. A through hole (505) is formed in the middle of the baffle (503). A push rod (501) is connected to the lower right side of the support frame (201) at a position on the right side of the baffle (503). The push rod (501) is in contact and cooperation with the baffle (503).
8. A rapid determination sampler for food allergens based on quantum dot labeling according to claim 7, characterized in that, It further includes a convex block (601) and a toothed ring (602). A plurality of convex blocks (601) are connected to the inner bottom of the limit frame (301) at intervals along the circumferential direction. A toothed ring (602) which is in clamping fit with the convex blocks (601) is connected to the outer side of the upper end of the sampling cylinder (103).