High-temperature-resistant optical dissolved oxygen electrode and preparation method thereof

Abstract

The invention provides a high-temperature-resistant optical dissolved oxygen electrode and a preparation method thereof. The high-temperature-resistant optical dissolved oxygen electrode is suitable for measuring dissolved oxygen under a 121 DEG C high-temperature steam sterilization condition. The electrode comprises a film cap shell, a refractor, a refractor fixing ring, a refractor sealing ring, a first temperature sensor, a second temperature sensor, a red light filter, a red fluorescence detector, a blue light filter, a blue LED lamp, a lamp holder, a PCB-1, a PCB-2, an electrode stem, a PG13.5 connector and a multi-layer sealing structure. The electrode ensures optical path stability and sealing performance in a high-temperature environment through a high-temperature resistant material, such as a PEEK electrode contact and an EPDM sealing ring. Temperature compensation is provided in real time through the first temperature sensor and the second temperature sensor, and measurement precision is maintained. The preparation method comprises the steps of element assembly, optical positioning, sealing installation and high-temperature test calibration, and solves the problems of poor high-temperature resistance and insufficient sealing performance in the prior art. The electrode is stable in performance after high-temperature sterilization, accurate and reliable in measurement and suitable for industries such as biological fermentation and cell culture.

Description

Technical Field

[0001] The present invention relates to the technical field of electrochemical sensors, and particularly to a high-temperature resistant optical dissolved oxygen electrode and a preparation method thereof. Background Art

[0002] Optical dissolved oxygen electrodes are important measuring devices in fields such as bioreactor fermentation, cell culture, and environmental monitoring, and they can achieve real-time monitoring of dissolved oxygen content through fluorescence or luminescence methods. Compared with traditional electrochemical dissolved oxygen electrodes, optical dissolved oxygen electrodes are widely used in industrial production and scientific research due to their advantages such as no need to consume oxygen, fast response speed, and simple maintenance. However, existing optical dissolved oxygen electrodes still have many deficiencies in terms of high-temperature resistance and sealing performance, and it is difficult to meet the strict requirements of repeated high-temperature sterilization in industries such as bioreactor fermentation and cell culture. Currently, optical dissolved oxygen electrodes mainly face the following problems during application: (1) Insufficient high-temperature resistance Traditional optical dissolved oxygen electrodes are prone to performance degradation problems during high-temperature sterilization (such as steam sterilization at 121°C). Solder joints of electronic components may become loose, resulting in unstable electrical connections; optical materials (such as light sources, detectors, and filters) are prone to physical deformation or changes in optical properties at high temperatures, leading to optical path deviation or signal attenuation, seriously affecting the measurement accuracy of dissolved oxygen. The insufficient stability of the mechanical structure in a high-temperature environment further exacerbates the risk of electrode failure.

[0003] (2) Poor sealing performance The sealing structure design of existing optical dissolved oxygen electrodes is unreasonable, or the sealing material deteriorates in a high-temperature steam environment, resulting in water vapor or moisture entering the electrode interior. The infiltration of water vapor may cause short circuits in electronic devices and moisture damage to optical components, thereby affecting the normal operation of the electrode and the accuracy and reliability of measurement data.

[0004] The above limitations make existing optical dissolved oxygen electrodes prone to damage in occasions that require high-temperature sterilization (such as bioreactor fermentation tanks or cell culture tanks), unable to work stably for a long time, and seriously restricting their popularization and application in related industries.

[0005] In response to the above problems, the current research and development of optical dissolved oxygen electrodes mainly focus on the following aspects: (1) Improving high-temperature resistance By selecting high-temperature resistant electronic components, optical materials, and structural designs, the stability of the electrode under 121°C high-temperature steam sterilization conditions is enhanced to ensure that the electrode can still maintain the stability of optical performance and electrical connection after high-temperature sterilization.

[0006] (2) Improving sealing performance Through the multi-layer sealing structure design and the selection of sealing materials with excellent high temperature tolerance (such as EPDM, PEEK, etc.), high-temperature steam can be effectively prevented from penetrating into the electrode, protecting electronic devices and optical components from damage, and ensuring long-term stable operation of the electrode.

[0007] (3) Simplified calibration method In order to meet the needs of rapid use after high-temperature sterilization, the electrode calibration method is optimized, the cumbersome operating steps are reduced, the measurement efficiency is improved, and the rapid recovery and application of the electrode after high-temperature sterilization is ensured.

[0008] Although optical dissolved oxygen electrodes have been widely used in related fields, the existing technology still has the following major problems: (1) Poor high temperature resistance The electronic components and optical materials of existing optical dissolved oxygen electrodes are not stable enough in high-temperature sterilization environments, and performance degradation often occurs. For example, the light source (such as LED) may lose luminous intensity at high temperatures, the optical filter may deform or fail, and the solder joints of electronic components may become loose, causing the electrode to not work properly and significantly reducing the measurement accuracy.

[0009] (2) Poor sealing performance The sealing structure design of the electrode is defective, or the selected sealing material has degraded performance in high temperature and high humidity environments, and cannot prevent steam or moisture from entering the electrode. The infiltration of water vapor may cause short circuits in electronic components and damage to optical components due to moisture, further affecting the reliability of the electrode and the accuracy of the measurement.

[0010] In summary, the optical dissolved oxygen electrode in the prior art is insufficient in terms of high temperature resistance and sealing performance, making it difficult to meet the requirements of long-term stable operation in application scenarios that require high temperature sterilization, such as biological fermentation and cell culture. Therefore, there is an urgent need for an optical dissolved oxygen electrode with good high temperature resistance, excellent sealing structure design and high-precision measurement capability to solve the problems existing in the prior art.

[0011] The present invention aims at addressing the defects in the above-mentioned prior art and proposes a high-temperature resistant optical dissolved oxygen electrode and a preparation method thereof. Through reasonable structural design, material selection and assembly process, the high-temperature resistance and sealing performance of the electrode are effectively improved, and the stability and measurement accuracy of the electrode after high-temperature sterilization are ensured, thereby meeting the actual application needs of industries such as biological fermentation and cell culture. Summary of the invention

[0012] To achieve the above object, the present invention provides a high temperature resistant optical dissolved oxygen electrode, comprising: Membrane cap housing A: used to form the optical channel at the front end of the electrode.

[0013] Refracting mirror fixing ring B and refracting mirror sealing ring D: Used to accurately position the refracting mirror C within the membrane cap housing A and provide sealing protection through the refracting mirror sealing ring D.

[0014] First temperature sensor E and second temperature sensor H: Arranged at different positions of the electrode respectively, monitoring temperature data in real time, providing temperature compensation to ensure measurement accuracy.

[0015] Optical components: Include a red filter F, a red fluorescence detector G, a blue filter I, and a blue LED lamp J. The red filter F and the red fluorescence detector G are installed on one side of the refracting mirror C, and the blue filter I and the blue LED lamp J are installed on the other side of the refracting mirror C. Precise positioning and fixation are achieved through the lamp holder K.

[0016] PCB components: Include PCB-1L and PCB-2T, which are respectively used to install electronic components, temperature sensors, and optical elements. PCB-1L and PCB-2T are connected by high-temperature solder to ensure stable and reliable electrical connection after high-temperature sterilization.

[0017] Sealing structure: Membrane cap sealing ring M: Set between the membrane cap housing A and the electrode rod N; Sealing ring O: Set inside the electrode rod N; Connector sealing ring Q: Set at the connection between the PG13.5 connector P and the electrode rod N; PCB housing sealing ring 1S and PCB housing sealing ring 2V: Set on the PCB housing 1R and the PCB housing 2U respectively; Electrode connector sealing ring Y: Set between the electrode connector X and the electrode connector housing Z.

[0018] The multi-layer sealing structure effectively prevents high-temperature steam from entering the electrode interior and protects the electronic and optical components.

[0019] Electrode connector X and electrode connector housing Z: Made of PEEK material, with good high-temperature resistance performance, and limited by the connector snap ring W to ensure structural stability and sealing.

[0020] Furthermore, the present invention also provides a preparation method for a high-temperature resistant optical dissolved oxygen electrode, including the following steps: Assembly of electronic components: Install electronic components such as the first temperature sensor E, the second temperature sensor H, the red fluorescence detector G, and the blue LED lamp J onto PCB-1L and PCB-2T, and perform high-temperature welding.

[0021] Installation of optical elements: Use a high-precision tooling to position the red filter F, the blue filter I, the refracting mirror C, and the light source, and fix them with high-temperature resistant glue.

[0022] Sealing structure installation: Install the membrane cap seal M, seal O, connector seal Q, PCB housing seal 1S, PCB housing seal 2V, and electrode connector seal Y in sequence to form a multi-layer sealing structure.

[0023] Assembly and gluing: Thread-connect each component and apply high-temperature and waterproof glue at the connection points to ensure the sealing performance.

[0024] Testing and calibration: Conduct high-temperature steam sterilization testing on the electrode and calibrate it in a standard dissolved oxygen solution to verify the measurement accuracy and sealing stability of the electrode.

[0025] The electrode prepared by the above method still has good sealing performance, high-temperature resistance performance, and measurement accuracy after high-temperature steam sterilization, meeting the actual requirements of optical dissolved oxygen measurement in industries such as biological fermentation and cell culture.

[0026] Advantages of the present invention: 1. Improved high-temperature stability: The special designs of the electronic components, optical sensing part, and electrode housing make their performance stable after high-temperature sterilization, ensuring the measurement accuracy.

[0027] 2. Good sealing performance: The multi-layer sealing structure and high-precision sealing process effectively prevent steam from entering the electrode interior and damaging the electronic devices and optical components, ensuring the reliability of the measurement results.

[0028] The optical dissolved oxygen electrode of the present invention with high-temperature sterilization resistance, without the need to add electrolyte, greatly simplifies the maintenance process, reduces the use cost, improves the working efficiency and measurement accuracy, on the premise of meeting the high-temperature sterilization or steam sterilization requirements of 110 to 135 degrees in the biological fermentation industry and cell culture industry. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] In order to more clearly illustrate the technical solutions in the present invention or the prior art, the following will briefly introduce the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings in the following description are only those of the present invention. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative efforts.

[0030] Figure 1 It is a schematic diagram of the overall structure of the electrode according to the embodiment of the present invention; Figure 2 It is a schematic diagram of the optical sensing part according to the embodiment of the present invention; Figure 3 It is a schematic cross-sectional view of the high-temperature resistant connector according to the embodiment of the present invention; Figure 4 It is a schematic diagram of the optical path installation method according to the embodiment of the present invention; Figure 5 Schematic diagram of the optical path installation method according to an embodiment of the present invention; Figure 6 Schematic diagram of the electrode joint installation method according to an embodiment of the present invention; Figure 7 Schematic diagram of the electrode joint installation method according to an embodiment of the present invention; Figure 8 Schematic diagram of the overall structural design of the housing according to an embodiment of the present invention.

[0031] The markings in the figure are: A, membrane cap housing; B, refractive mirror fixing ring; C, refractive mirror; D, refractive mirror sealing ring; E, first temperature sensor; F, red filter; G, red fluorescence detector; H, second temperature sensor; I, blue filter; J, blue LED lamp; K, lamp holder; L, PCB-1; M, membrane cap sealing ring; N, electrode rod; O, sealing ring; P, PG13.5 connector; Q, connector sealing ring; R, PCB housing 1; S, PCB housing sealing ring 1; T, PCB-2; U, PCB housing 2; V, PCB housing sealing ring 2; W, joint snap ring; X, electrode joint; Y, electrode joint sealing ring; Z, electrode joint housing. Detailed implementation manners

[0032] The present invention will be described in detail below with reference to the accompanying drawings and specific embodiments. At the same time, it should be noted here that in order to make the embodiments more detailed, the following embodiments are the best and preferred embodiments. For some well-known technologies, those skilled in the art can also adopt other alternative methods for implementation; and the accompanying drawings are only for more specifically describing the embodiments, and are not intended to specifically limit the present invention.

[0033] It should be pointed out that in the specification, when referring to "an embodiment", "embodiment", "exemplary embodiment", "some embodiments", etc., it indicates that the described embodiment may include specific features, structures or characteristics, but not necessarily every embodiment includes such specific features, structures or characteristics. In addition, when combining an embodiment to describe a specific feature, structure or characteristic, implementing such a feature, structure or characteristic in combination with other embodiments (whether explicitly described or not) should be within the knowledge of those skilled in the relevant art.

[0034] Generally, the terms can be understood at least in part from their use in the context. For example, at least in part depending on the context, the term "one or more" used herein can be used to describe any feature, structure or characteristic in a singular sense, or can be used to describe a combination of features, structures or characteristics in a plural sense. In addition, the term "based on" can be understood as not necessarily intended to convey a set of exclusive factors, but instead, at least in part depending on the context, allowing the existence of other factors that may not be explicitly described.

[0035] Specifically refer to Figure 1 、 Figure 2 、 Figure 3 and Figure 8 as shown in the content of, Overall structure design of the electrode The electrode of the present invention is composed of a membrane cap housing A, a refractive mirror fixing ring B, a refractive mirror C, a refractive mirror sealing ring D, a first temperature sensor E, a red filter F, a red fluorescence detector G, a second temperature sensor H, a blue filter I, a blue LED lamp J, a lamp holder K, a PCB-1 L, a membrane cap sealing ring M, an electrode rod N, a sealing ring O, a PG13.5 connector P, a connector sealing ring Q, a PCB housing 1 R, a PCB housing sealing ring 1 S, a PCB-2 T, a PCB housing 2 U, a PCB housing sealing ring 2 V, a joint snap ring W, an electrode joint X, an electrode joint sealing ring Y, and an electrode joint housing Z, etc.

[0036] Membrane cap housing A: As the housing at the front end of the electrode, an optical channel is formed inside.

[0037] Refractive mirror C, refractive mirror fixing ring B, refractive mirror sealing ring D: Precisely fix the refractive mirror C in the membrane cap housing A, and form a seal through the refractive mirror sealing ring D to ensure that the optical path is not affected by external steam.

[0038] First temperature sensor E, second temperature sensor H: Are respectively located at different positions inside the electrode, used to monitor temperature data in real time, provide temperature compensation, and ensure the accuracy of dissolved oxygen measurement.

[0039] Optical components: The red filter F and the blue filter I respectively cooperate with the red fluorescence detector G and the blue LED lamp J, and are precisely positioned and fixed by the lamp holder K, and specific wavelength light excitation and detection are carried out through the optical path of the refractive mirror C.

[0040] PCB components: PCB-1 L and PCB-2 T are installed with electronic components, including the first temperature sensor E, the second temperature sensor H, optical elements G and J, and the two PCBs are connected by high-temperature solder and high-temperature resistant wires.

[0041] Sealing structure: The membrane cap sealing ring M, the sealing ring O, the connector sealing ring Q, the PCB housing sealing ring 1 S, the PCB housing sealing ring 2 V, and the electrode joint sealing ring Y respectively form a multi-layer sealing structure at different parts to prevent high-temperature steam from entering the interior.

[0042] Electrode joint X and electrode joint housing Z: Adopt PEEK material to ensure the electrical stability and overall sealing performance of the electrode joint in a high-temperature steam environment.

[0043] Preparation method of the electrode (1) Assembly of electronic components Weld the first temperature sensor E, the second temperature sensor H, the red fluorescence detector G, the blue LED lamp J and other electronic components onto PCB-1L and PCB-2T. Use high-temperature solder during the welding process to ensure that the solder joints do not become loose under high-temperature sterilization at 121°C.

[0044] (2) Installation of optical components Use a tooling (accuracy ±0.02) to accurately position the blue filter I and the blue LED lamp J, and the red filter F and the red fluorescence detector G within the membrane cap housing A.

[0045] Install the refractive mirror C at the designated position in the membrane cap housing A, fix it with the refractive mirror fixing ring B, and ensure the sealing performance through the refractive mirror sealing ring D.

[0046] After the blue LED lamp J and the red fluorescence detector G are fixed, apply an appropriate amount of high-temperature resistant glue to the rear end for preliminary fixation. After the glue cures, ensure the stability of the optical path.

[0047] (3) Installation of the sealing structure Install the membrane cap sealing ring M between the membrane cap housing A and the electrode rod N.

[0048] Install the sealing ring O inside the electrode rod N, and install the connector sealing ring Q at the connection between the PG13.5 connector P and the electrode rod N.

[0049] Install the PCB housing sealing ring 1S around the PCB housing 1R, and install the PCB housing sealing ring 2V between the PCB housing 2U and PCB-2T.

[0050] Install the electrode connector sealing ring Y outside the electrode connector X, and tighten and seal the electrode connector housing Z with the electrode connector X.

[0051] (4) Thread gluing and connector fixing Apply high-temperature resistant and waterproof glue evenly to all threaded connections, and use the connector snap ring W to restrict the axial displacement of the electrode connector X to ensure that there is no loosening or air leakage after high-temperature sterilization.

[0052] (5) High-temperature sterilization test and calibration Conduct multiple high-temperature steam sterilization tests at 121°C on the assembled electrode to verify the sealing performance and the stability of the optical and electronic components.

[0053] Place the electrode in a standard solution for calibration, and use the first temperature sensor E and the second temperature sensor H to provide temperature data for real-time compensation to ensure the accuracy and stability of dissolved oxygen measurement.

[0054] As Figure 4 and Figure 5 shown The optical path installation method is as follows Due to the high requirements for the optical path, the blue filter A and the blue LED lamp are preliminarily fixed on the housing by using a tooling (accuracy ±0.02) for positioning, and then Figure 8 an appropriate amount of high-temperature glue is applied and fixed behind the blue LED lamp shown; the red filter C and the red fluorescence detector D are preliminarily fixed on the housing by using a tooling (accuracy ±0.02), and then the pins of the red fluorescence detector D are soldered and fixed on the PCB.

[0055] Such as Figure 6 and Figure 7 shown The optical path installation method is as follows Electrode connector installation First, put the sealing ring B on the housing A, insert the sealing ring D into the housing E, then install the housing C on the slot of the housing B, align the electrode connector D with the slot of the housing C, and finally apply an appropriate amount of high-temperature glue at the thread of the housing B, put the housing E on the electrode connector D, and screw it clockwise and fix it on the thread of the housing B. After tightening, wait for the glue to dry and fix.

[0056] Example: Example 1: Stability test under high-temperature sterilization conditions Place the optical dissolved oxygen electrode prepared according to the above steps in a high-temperature steam environment of 121°C for 30 minutes of sterilization time, and sterilize continuously for 20 times. After each sterilization, perform the following detections: Check the sealing conditions of the membrane cap housing A, the electrode rod N, the electrode connector X, and all the sealing rings M, O, Q, S, V, Y, and there is no steam infiltration phenomenon.

[0057] Measure the transmission stability of the optical signal, and there is no obvious attenuation in the intensity of the excitation light emitted by the blue LED lamp J, and the detection sensitivity of the red fluorescence detector G remains stable.

[0058] In a standard dissolved oxygen solution, calibrate the electrode and measure the dissolved oxygen content, and the error is less than ±0.5%.

[0059] Result: The optical dissolved oxygen electrode of the present invention has good sealing performance and the stability of optical and electronic components under high-temperature sterilization conditions, and can meet the requirements of long-term repeated high-temperature sterilization use.

[0060] Example 2: Measurement accuracy in practical applications Apply the calibrated optical dissolved oxygen electrode to a bioreactor, with the temperature in the tank being 40°C and the standard value of dissolved oxygen being 6.5 mg / L.

[0061] The electrode monitors the dissolved oxygen content in real time, and uses the first temperature sensor E and the second temperature sensor H to provide temperature data for compensation.

[0062] Continuous monitoring for 48 hours, the deviation between the measured value of dissolved oxygen and the standard value does not exceed ±0.2 mg / L.

[0063] Results: The electrode of the present invention shows high measurement accuracy and stability in practical applications and is suitable for dissolved oxygen measurement in high-temperature sterilization scenarios.

[0064] Through the description of the above specific embodiments and examples, the high-temperature-resistant optical dissolved oxygen electrode and its preparation method of the present invention solve problems such as the sealing performance, stability, and measurement accuracy of the electrode after high-temperature sterilization, are applicable to scenarios such as biological fermentation and cell culture that require high-temperature sterilization, and have broad industrial application value.

[0065] The present invention covers any substitutions, modifications, equivalent methods, and solutions made within the spirit and scope of the present invention. For the public to have a thorough understanding of the present invention, specific details are described in detail in the following preferred embodiments of the present invention, and those skilled in the art can fully understand the present invention without the description of these details. Additionally, well-known methods, processes, procedures, components, and circuits, etc. are not described in detail to avoid unnecessary confusion to the essence of the present invention.

[0066] The above are only the preferred embodiments of the present invention. It should be noted that for those of ordinary skill in the art, without departing from the principle of the present invention, several improvements and refinements can be made, and these improvements and refinements should also be regarded as the protection scope of the present invention.

Claims

1. A high-temperature resistant optical dissolved oxygen electrode, comprising a membrane cap housing (A), a first temperature sensor (E), a second temperature sensor (H), a refractive mirror fixing ring (B), and a refractive mirror sealing ring (D), characterized in that, Comprising: A membrane cap housing (A), inside which an optical channel for optical signal transmission is formed; A refractive mirror fixing ring (B) and a refractive mirror sealing ring (D) are installed inside the membrane cap housing (A). The refractive mirror fixing ring (B) is used to fix the refractive mirror (C) in a predetermined position, and the refractive mirror sealing ring (D) forms a seal between the refractive mirror (C) and the membrane cap housing (A) to prevent high-temperature steam from entering the electrode interior; A first temperature sensor (E) and a second temperature sensor (H) are both arranged at predetermined positions inside the membrane cap housing (A), for monitoring the temperature inside the electrode in real time during high-temperature sterilization and dissolved oxygen measurement, and providing temperature compensation data to the measuring instrument; A red filter (F) and a red fluorescence detector (G) are arranged in cooperation on one side of the refractive mirror (C), and a blue filter (I) and a blue LED lamp (J) are arranged in cooperation on the other side of the refractive mirror (C). The red filter (F) and the red fluorescence detector (G), and the blue filter (I) and the blue LED lamp (J) are fixed by high-precision (±0.02) positioning and high-temperature resistant glue curing, ensuring the accuracy of the optical path and the stability of the measurement signal are still maintained after 121°C high-temperature steam sterilization; A lamp holder (K) is fixed inside the membrane cap housing (A), for stably supporting and positioning the blue LED lamp (J) and the red fluorescence detector (G) under high-temperature steam sterilization conditions to maintain the spatial position and optical path accuracy of the optical components; PCB-1 (L) and PCB-2 (T) are printed circuit board assemblies installed successively along the longitudinal direction of the electrode, for carrying the first temperature sensor (E), the second temperature sensor (H), the red fluorescence detector (G), the blue LED lamp (J) and other high-temperature resistant electronic components. PCB-1 (L) and PCB-2 (T) are connected by high-temperature solder and high-temperature resistant wires, ensuring the electrical characteristics and signal transmission are still reliable after multiple 121°C high-temperature steam sterilizations; A membrane cap sealing ring (M) is arranged between the membrane cap housing (A) and the electrode rod (N). The membrane cap sealing ring (M) blocks water vapor from entering the inside of the membrane cap housing (A) in a high-temperature steam environment, thereby protecting the internal optical and electronic components; The electrode rod (N) is coaxially connected to the membrane cap housing (A), and a sealing ring (O) is arranged inside the electrode rod (N). The sealing ring (O) cooperates with the electrode rod (N) and the membrane cap housing (A) to form a sealing structure to maintain the airtightness and structural stability inside the electrode after high-temperature sterilization; A PG13.5 connector (P) is arranged at the rear of the electrode rod (N), for the standard connection between the electrode and an external measuring instrument. A connector sealing ring (Q) is arranged at the connection between the PG13.5 connector (P) and the electrode rod (N). The connector sealing ring (Q) prevents high-temperature steam from entering the electrode interior along this interface, ensuring the measurement accuracy and reliability after high-temperature sterilization; The PCB housing 1 (R) covers part of the areas of PCB-1 (L) and PCB-2 (T), and a PCB housing sealing ring 1 (S) is arranged around the PCB housing 1 (R). The PCB housing sealing ring 1 (S) can still effectively block the intrusion of steam into the PCB area under high-temperature environment, protecting the stable function of electronic components. The PCB housing 2 (U) wraps PCB-2 (T), and a PCB housing sealing ring 2 (V) is arranged between the PCB housing 2 (U) and PCB-2 (T). The PCB housing sealing ring 2 (V) provides effective sealing during 121°C high-temperature steam sterilization, avoiding water vapor from damaging the components on PCB-2 (T). The joint snap ring (W) is installed between the electrode joint (X) and the PG13.5 connector (P), used to limit the displacement of the electrode joint (X) in the axial direction, ensuring that the electrode joint (X) still remains firmly and accurately positioned after high-temperature sterilization. The electrode joint (X) is integrally formed by PEEK plastic and a copper pin. An electrode joint sealing ring (Y) is arranged on the outer periphery of the electrode joint (X). The electrode joint sealing ring (Y) forms a seal between the electrode joint (X) and the electrode joint housing (Z). The electrode joint housing (Z) is hermetically connected to the PG13.5 connector (P) and the electrode rod (N) by any one of the thread or snap methods, and a high-temperature resistant waterproof glue is applied at the connection to still avoid water vapor from entering the internal optical and electronic component area under the condition of 121°C high-temperature steam sterilization, maintaining the dissolved oxygen measurement accuracy and stability of the electrode.

2. The preparation method of the high-temperature resistant optical dissolved oxygen electrode according to claim 1, characterized in that, It includes the following steps: S1. Electronic component assembly: Install the first temperature sensor (E), the second temperature sensor (H), the red fluorescence detector (G), the blue LED lamp (J) and other high-temperature resistant electronic components on PCB-1 (L) and PCB-2 (T) according to the circuit design requirements, and use high-temperature solder for welding to ensure that the solder joints do not become loose after multiple 121°C high-temperature steam sterilizations. S2. Optical component installation: Use a precision tooling (accuracy ±0.02) to accurately position the red filter (F) with the red fluorescence detector (G), and the blue filter (I) with the blue LED lamp (J). Fix the refractive mirror (C) at a predetermined position in the membrane cap housing (A) through the refractive mirror fixing ring (B) and the refractive mirror sealing ring (D), and perform preliminary fixation by applying high-temperature resistant glue. After the glue cures, ensure the accuracy of the optical path. S3. Installation of multi-layer sealing structure: Install a membrane cap sealing ring (M) between the membrane cap housing (A) and the electrode rod (N), install a sealing ring (O) inside the electrode rod (N), install a connector sealing ring (Q) at the connection between the PG13.5 connector (P) and the electrode rod (N), install a PCB housing sealing ring 1 (S) around the PCB housing 1 (R), install a PCB housing sealing ring 2 (V) between the PCB housing 2 (U) and PCB-2 (T), and install an electrode joint sealing ring (Y) between the electrode joint (X) and the electrode joint housing (Z). S4. Gluing and fastening: Apply high-temperature resistant waterproof glue to each threaded connection of the electrode to maintain the bonding performance at a high temperature of 200 °C, and limit the axial displacement of the electrode joint (X) through the joint clamp ring (W) to ensure that the overall structure of the electrode remains tightly sealed and stable after high-temperature sterilization; S5. Testing and calibration: Subject the assembled electrode to multiple high-temperature steam sterilization tests at 121 °C. After confirming that each sealing ring of the membrane cap sealing ring (M), sealing ring (O), connector sealing ring (Q), PCB housing sealing ring 1 (S), PCB housing sealing ring 2 (V), electrode joint sealing ring (Y) and the high-temperature resistant glue still maintain the sealing performance and stability in the high-temperature steam environment, place the electrode into a standard solution for calibration, and perform temperature compensation using the temperature data provided by the first temperature sensor (E) and the second temperature sensor (H) to obtain accurate results in actual dissolved oxygen measurement.

3. The preparation method according to claim 2, characterized in that, In steps (1) and (2), select optical components (refraction mirror (C), red filter (F), blue filter (I)) and optoelectronic components (red fluorescence detector (G), blue LED lamp (J)), the first temperature sensor (E), the second temperature sensor (H) and corresponding circuit components that meet the high-temperature resistance requirements to ensure that these components can still maintain the predetermined characteristics and functions after multiple high-temperature steam sterilizations at 121 °C.

4. The preparation method according to claim 2 or 3, characterized in that, In steps (3) and (4), use special tooling and torque control tools to install the membrane cap sealing ring (M), sealing ring (O), connector sealing ring (Q), PCB housing sealing ring 1 (S), PCB housing sealing ring 2 (V) and electrode joint sealing ring (Y), and strictly control the glue application amount and tightening torque of the high-temperature resistant waterproof glue to ensure that there is no air leakage or water vapor intrusion in the electrode during high-temperature sterilization and subsequent use.

5. The preparation method according to any one of claims 2-4, characterized in that, The high-temperature sterilization test in step (5) can be repeated multiple times to verify that the electrode still has high sealing performance and high-precision dissolved oxygen measurement performance after long-term and repeated high-temperature steam sterilizations at 121 °C.

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