Liquid path system of full-automatic electrochemical luminescence immunoassay analyzer

Abstract

The invention discloses a liquid path system of a full-automatic electrochemical luminescence immunoassay analyzer, and belongs to the field of chemiluminescence immunoassay, a liquid supply water tank is communicated with a first centrifugal pump, part of liquid extracted by the first centrifugal pump forms system liquid for cleaning, part of liquid extracted by the first centrifugal pump is degassed by a degassing device to form low-pressure degassed water, and the low-pressure degassed water is used for cleaning the system liquid; the gear pump pressurizes part of the low-pressure degassing water to form high-pressure degassing water; the high-pressure degassing water flows to the sample adding needle, the reagent needle cleans the inner wall, the system liquid flows to the sample adding needle cleaning station, the reagent needle cleaning station and the needle group cleaning station to clean the outer wall of the needle, and the cleaning liquid in the cleaning liquid bottle flows to the sample adding needle cleaning liquid storage station and the reagent needle cleaning liquid storage station to clean the reagent on the inner wall of the needle; the low-pressure degassing water flows to the buffer solution control module to control the buffer solution in the buffer solution bottle to flow to the distribution needle and the liquid suction needle; by means of the design, the liquid path system is high in precision, good in reliability and high in repeatability.

Description

Technical Field

[0001] The present invention relates to the field of chemiluminescence immunoassay, and particularly to the liquid path system of a fully automatic electrochemiluminescence immunoassay analyzer. Background Art

[0002] Luminescence immunoassay is an analytical method in which a sample is mixed with a variety of reagents including magnetic particles and enzyme labels, and after an antigen-antibody reaction occurs, a chemiluminescent substrate is added, and immunoassay is performed based on the luminescence intensity.

[0003] In existing fully automatic luminescence immunoassay analyzers, a sampling needle is generally used to transfer samples and reagents quantitatively in sequence for mixing, and after each sampling by the sampling needle, a cleaning solution is used for cleaning. Most of the reasons for the failure of dark sample detection during the operation of chemiluminescence measurement instruments are attributed to liquid path system failures, and the failure reasons mainly include poor quantitative accuracy of the liquid path module, low reliability of each module in the system, and poor repeatability of each module in the test process, etc. Summary of the Invention

[0004] In order to overcome the deficiencies of the prior art, one of the objectives of the present invention is to provide a liquid path system for a fully automatic electrochemiluminescence immunoassay analyzer with high precision, good reliability, and high repeatability.

[0005] One of the objectives of the present invention is achieved by adopting the following technical solution:

[0006] The liquid path system of a fully automatic electrochemiluminescence immunoassay analyzer includes a liquid supply module, a sample sampling module, a reagent sampling module, a cleaning and separation module, and a detection module.

[0007] The liquid supply module includes a liquid supply water tank, a first centrifugal pump, a degassing device, and a gear pump. The liquid supply water tank is connected to the first centrifugal pump. The first centrifugal pump extracts the liquid in the liquid supply water tank. A part of the liquid extracted by the first centrifugal pump forms system liquid for cleaning. The degassing device is connected to the first centrifugal pump. A part of the liquid extracted by the first centrifugal pump is degassed by the degassing device to form low-pressure degassed water. The gear pump is connected to the degassing device. The gear pump pressurizes a part of the low-pressure degassed water to form high-pressure degassed water.

[0008] The sample loading module includes a loading needle, a loading needle cleaning station, a cleaning liquid bottle, and a loading needle cleaning liquid storage station. The loading needle is connected to the gear pump. The high-pressure degassed water flows to the loading needle to clean the inner wall of the loading needle. The loading needle cleaning station is connected to the first centrifugal pump, and the system liquid flows into the loading needle cleaning station to clean the outer wall of the loading needle. The cleaning liquid bottle is connected to the loading needle cleaning liquid storage station, and the cleaning liquid in the cleaning liquid bottle flows to the loading needle cleaning liquid storage station to clean the reagent on the inner wall of the loading needle;

[0009] The reagent loading module includes a reagent needle, a reagent needle cleaning station, and a reagent needle cleaning liquid storage station. The reagent needle is connected to the gear pump. The high-pressure degassed water flows to the reagent needle to clean the inner wall of the reagent needle. The reagent needle cleaning station is connected to the first centrifugal pump, and the system liquid flows into the reagent needle cleaning station to clean the outer wall of the reagent needle. The reagent needle cleaning liquid storage station is connected to the cleaning liquid bottle, and the cleaning liquid in the cleaning liquid bottle flows to the reagent needle cleaning liquid storage station to clean the reagent on the inner wall of the reagent needle;

[0010] The cleaning and separation module includes a dispensing needle, a liquid suction needle, a buffer solution bottle, a buffer solution control module, and a needle group cleaning station. The buffer solution control module is connected to the degassing device, and the low-pressure degassed water flows to the buffer solution control module. The buffer solution control module controls the buffer solution in the buffer solution bottle to flow to the dispensing needle and the liquid suction needle. The needle group cleaning station is connected to the first centrifugal pump, and the system liquid flows into the needle group cleaning station to clean the outer walls of the dispensing needle and the liquid suction needle;

[0011] The detection module includes a measurement cell, a suction needle, a co-reactant liquid plunger pump, a co-reactant liquid bottle, and a co-reactant liquid buffer station. The measurement cell is connected to the degassing device, and the low-pressure degassed water flows to the measurement cell. The suction needle is connected to the measurement cell. The co-reactant liquid plunger pump and the cleaning liquid plunger pump are respectively connected to the degassing device, and the co-reactant liquid plunger pump controls the co-reactant liquid bottle to supply liquid to the co-reactant liquid buffer station.

[0012] Further, the liquid supply module further includes a five-way valve, a first throttle valve, and a pressure gauge. The five-way valve is respectively connected to the first centrifugal pump, the degassing device, the liquid supply water tank, and the pressure gauge. The liquid supply water tank, the first centrifugal pump, and the five-way valve form a loop. The first throttle valve is installed on the loop, and the opening and closing degree of the first throttle valve is adjusted to adjust the loop pressure according to the reading of the pressure gauge.

[0013] Further, the liquid supply module further includes a refrigerated water tank and a first three-way joint. The first three-way joint is respectively connected to the five-way valve, the refrigerated water tank, and the degassing device.

[0014] Further, the liquid supply module further includes a second three-way joint, a first six-way valve block, a four-way valve, and a second six-way valve block. The second three-way joint is respectively connected to the output end of the degassing device, the input end of the gear pump, and the first six-way valve block. The low-pressure degassed water formed by the degassing of the degassing device flows into the first six-way valve block through the second three-way joint, and the first six-way valve block outputs the low-pressure degassed water; the low-pressure degassed water formed by the degassing of the degassing device flows into the gear pump through the second three-way joint for pressurization, and the formed high-pressure degassed water flows to the second six-way valve block through the four-way valve, and the second six-way valve block outputs the high-pressure degassed water.

[0015] Further, the sample loading module further includes a sample loading plunger pump and a clot detection structure. The sample loading plunger pump is connected to the second six-way valve block. The sample loading plunger pump controls the high-pressure degassed water to flow to the reagent needle. The clot detection structure is located between the sample loading plunger pump and the reagent needle, and the clot detection structure detects the high-pressure degassed water.

[0016] Further, the sample loading module further includes a third six-way valve block, a third solenoid valve, and a pressure regulating valve. The third six-way valve block is communicated with the first three-way joint. The third solenoid valve and the pressure regulating valve are installed between the third six-way valve block and the sampling needle cleaning station.

[0017] Further, the reagent loading module further includes a fifth solenoid valve, a reagent loading plunger pump, and a sixth solenoid valve. The reagent loading plunger pump is communicated with the second six-way valve block. The fifth solenoid valve is located between the second six-way valve block and the reagent loading plunger pump. The reagent loading plunger pump controls the cleaning of the reagent needle. The reagent needle cleaning station is communicated with the third six-way valve block. The sixth solenoid valve is located between the reagent needle cleaning station and the third six-way valve block.

[0018] Further, the cleaning and separation module further includes an eighth solenoid valve and a pre-cleaning plunger pump. The pre-cleaning plunger pump is communicated with the first six-way valve block. The eighth solenoid valve is arranged between the first six-way valve block and the pre-cleaning plunger pump. The pre-cleaning plunger pump is connected to the buffer control module to control the buffer solution in the buffer solution bottle.

[0019] Further, the detection module further includes a cleaning liquid plunger pump and a sampling needle cleaning station. The sample loading module further includes a cleaning liquid control module. The cleaning liquid plunger pump is connected to the cleaning liquid control module. The cleaning liquid control module is communicated with the cleaning liquid bottle. The cleaning liquid plunger pump controls the cleaning liquid to flow from the cleaning liquid bottle into the sampling needle cleaning station through the cleaning liquid control module.

[0020] Further, the liquid path system of the fully automatic electrochemiluminescence immunoassay analyzer further includes a refrigeration module. The refrigeration module includes a reagent bin and a second centrifugal pump. The reagent bin and the second centrifugal pump are connected to the refrigeration water tank. The refrigeration water tank stores the system liquid and cools the system liquid. The second centrifugal pump extracts the cooled system liquid in the refrigeration water tank to the reagent bin for refrigeration.

[0021] Compared with the prior art, in the liquid path system of the fully automatic electrochemiluminescence immunoassay analyzer of the present invention, the liquid supply water tank of the liquid supply module is communicated with the first centrifugal pump. The first centrifugal pump extracts the liquid in the liquid supply water tank. A part of the liquid extracted by the first centrifugal pump forms the system liquid for cleaning. The degassing device is communicated with the first centrifugal pump. A part of the liquid extracted by the first centrifugal pump is degassed by the degassing device to form low-pressure degassed water. The gear pump is communicated with the degassing device. The gear pump pressurizes a part of the low-pressure degassed water to form high-pressure degassed water; the sampling needle of the sample sampling module is communicated with the gear pump. The high-pressure degassed water flows to the sampling needle to clean the inner wall of the sampling needle. The sampling needle cleaning station is communicated with the first centrifugal pump. The system liquid flows into the sampling needle cleaning station to clean the outer wall of the sampling needle. The cleaning liquid bottle is communicated with the sampling needle cleaning liquid storage station. The cleaning liquid in the cleaning liquid bottle flows to the sampling needle cleaning liquid storage station to clean the reagent on the inner wall of the sampling needle; the reagent needle of the reagent sampling module is communicated with the gear pump. The high-pressure degassed water flows to the reagent needle to clean the inner wall of the reagent needle. The reagent needle cleaning station is communicated with the first centrifugal pump. The system liquid flows into the reagent needle cleaning station to clean the outer wall of the reagent needle. The reagent needle cleaning liquid storage station is communicated with the cleaning liquid bottle. The cleaning liquid in the cleaning liquid bottle flows to the reagent needle cleaning liquid storage station to clean the reagent on the inner wall of the reagent needle; the buffer control module of the cleaning and separation module is communicated with the degassing device. The low-pressure degassed water flows to the buffer control module. The buffer control module controls the flow of the buffer liquid in the buffer liquid bottle to the dispensing needle and the liquid suction needle. The needle group cleaning station is communicated with the first centrifugal pump. The system liquid flows into the needle group cleaning station to clean the outer walls of the dispensing needle and the liquid suction needle; the measuring cell of the detection module is communicated with the degassing device. The low-pressure degassed water flows to the measuring cell. The aspiration needle is connected to the measuring cell. The co-reaction liquid plunger pump and the cleaning liquid plunger pump are respectively communicated with the degassing device. The co-reaction liquid plunger pump controls the supply of the co-reaction liquid in the co-reaction liquid bottle to the co-reaction liquid buffer station. Through the above design, the liquid path system of the fully automatic electrochemiluminescence immunoassay analyzer has high precision, good reliability and high repeatability. Description of the Drawings

[0022] Figure 1 It is a schematic structural diagram of the liquid path system of the fully automatic electrochemiluminescence immunoassay analyzer of the present invention;

[0023] Figure 2 is Figure 1 a schematic diagram of the liquid supply module of the liquid path system of the fully automatic electrochemiluminescence immunoassay analyzer;

[0024] Figure 3 is Figure 1 a schematic diagram of the sample loading module of the liquid path system of a fully automatic electrochemiluminescence immunoassay analyzer;

[0025] Figure 4 is Figure 1 a schematic diagram of the reagent loading module of the liquid path system of a fully automatic electrochemiluminescence immunoassay analyzer;

[0026] Figure 5 is Figure 1 a schematic diagram of the cleaning and separation module of the liquid path system of a fully automatic electrochemiluminescence immunoassay analyzer;

[0027] Figure 6 is Figure 1 a schematic diagram of the refrigeration module of the liquid path system of a fully automatic electrochemiluminescence immunoassay analyzer;

[0028] Figure 7 is Figure 1 a schematic diagram of the detection module of the liquid path system of a fully automatic electrochemiluminescence immunoassay analyzer.

[0029] In the figure: 10, liquid supply module; 11, filter; 12, first solenoid valve; 13, liquid supply water tank; 14, first centrifugal pump; 15, five-way valve; 16, first throttle valve; 17, first three-way joint; 18, refrigeration water tank; 19, second throttle valve; 191, degassing device; 192, second three-way joint; 193, first six-way valve block; 194, gear pump; 195, four-way valve; 196, second six-way valve block; 20, sample loading module; 21, second solenoid valve; 22, sample loading plunger pump; 23, clot detection structure; 24, sampling needle; 250, third six-way valve block; 251, third solenoid valve; 252, pressure regulating valve; 253, sampling needle cleaning station; 26, cleaning liquid bottle; 27, cleaning liquid control module; 28, fourth solenoid valve; 29, sampling needle cleaning liquid storage station; 30, reagent loading module; 31, fifth solenoid valve; 32, reagent loading plunger pump; 33, reagent needle; 34, sixth solenoid valve; 35, reagent needle cleaning station; 36, seventh solenoid valve; 37, reagent needle cleaning liquid storage station; 40, cleaning and separation module; 41, eighth solenoid valve; 42, pre-cleaning plunger pump; 43, buffer solution control module; 430, ninth solenoid valve; 431, tenth solenoid valve; 432, eleventh solenoid valve; 433, fifteenth solenoid valve; 44, buffer solution bottle; 45, dispensing needle; 46, liquid suction needle; 47, twelfth solenoid valve; 48, first preheating device; 49, needle group cleaning station; 50, refrigeration module; 51, reagent warehouse; 52, second centrifugal pump; 60, detection module; 61, thirteenth solenoid valve; 62, liquid suction needle plunger pump; 63, tube valve clamp; 64, measuring cell; 65, liquid suction needle; 66, fourteenth solenoid valve; 67, co-reactant liquid plunger pump; 68, cleaning liquid plunger pump; 69, co-reactant liquid bottle; 691, co-reactant liquid control module; 692, second preheating device; 693, co-reactant liquid buffer station; 694, liquid suction needle cleaning station. Detailed implementation mode

[0030] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0031] It should be noted that when a component is referred to as "fixed to" another component, it can be directly on the other component or there may be another intermediate component through which it is fixed. When a component is considered to be "connected to" another component, it can be directly connected to the other component or there may be another intermediate component at the same time. When a component is considered to be "disposed on" another component, it can be directly disposed on the other component or there may be another intermediate component at the same time. The terms "vertical", "horizontal", "left", "right" and similar expressions used in this article are only for the purpose of illustration.

[0032] Unless otherwise defined, all technical and scientific terms used in this article have the same meaning as those commonly understood by those skilled in the technical field to which the present invention belongs. The terms used in the description of the present invention in this article are only for the purpose of describing specific embodiments and are not intended to limit the present invention. The term "and / or" used in this article includes any and all combinations of one or more of the related listed items.

[0033] Please refer to Figure 1 , the liquid path system of the fully automatic electrochemiluminescence immunoassay analyzer includes a liquid supply module 10, a sample loading module 20, a reagent loading module 30, a cleaning and separation module 40, a refrigeration module 50, and a detection module 60.

[0034] Please continue to refer to Figure 2 , the liquid supply module 10 includes a filter 11, a first solenoid valve 12, a liquid supply water tank 13, a first centrifugal pump 14, a five-way valve 15, a first throttle valve 16, a first three-way joint 17, a refrigeration water tank 18, a second throttle valve 19, a degassing device 191, a second three-way joint 192, a first six-way valve block 193, a gear pump 194, a four-way valve 195, and a second six-way valve block 196.

[0035] The filter 11 is connected to the first solenoid valve 12, and the first solenoid valve 12 is connected to the liquid supply water tank 13. Deionized water enters the liquid supply water tank 13 for caching through the filter 11 and the first solenoid valve 12. The liquid supply water tank 13 is connected to the five-way valve 15 through the first centrifugal pump 14. One end of the five-way valve 15 is connected to the liquid supply water tank 13 through the first throttle valve 16 to form a loop, and a pressure gauge is installed on the five-way valve 15. Specifically, the first centrifugal pump 14 is a magnetic centrifugal pump. Under the action of the magnetic centrifugal pump, deionized water returns to the liquid supply water tank 13 through the five-way valve 15 to form a loop, and the opening and closing degree of the first throttle valve 16 is adjusted so that the reading of the pressure gauge on the five-way valve 15 is 60 KPa.

[0036] One end of the first three-way joint 17 is connected to the five-way valve 15, the other end is connected to the refrigeration water tank 18, and the last end is communicated with the second throttle valve 19 and the degassing device 191. The system liquid flowing out of the first three-way joint 17 is divided into two paths. One path is supplied to the refrigeration water tank 18 through the switching valve, and the other path passes through the switching valve and is divided into two paths again through the three-way joint. One of them is supplied to each cleaning station through the second throttle valve 19 for cleaning the outer walls of the reagent needle, the sampling needle and the cleaning separation needle. The other path forms low-pressure degassed water after being processed by the degassing device 191. The low-pressure degassed water forms two paths through the second three-way joint 192. One path outputs the low-pressure degassed water through the first six-way valve block 193 to provide low-pressure degassed water for the pre-cleaning plunger pump 42 of the cleaning separation module 40 and the sampling needle plunger pump, the co-reaction liquid plunger pump 67, and the cleaning liquid plunger pump 68 of the detection module 60. The other path forms high-pressure degassed water after being accurately pressurized by the gear pump 194. The high-pressure degassed water is then supplied to the sampling needle plunger pump 22 of the sample sampling module 20 and the reagent sampling plunger pump 32 of the reagent sampling module 30 through the four-way valve 195 and the second six-way valve block 196 for high-pressure flushing of the inner wall of the needle, and is supplied to the sampling needle cleaning station 694 of the detection module 60 for high-pressure flushing of the outer wall of the sampling needle 65. One branch of the four-way valve 195 after being pressurized by the gear pump 194 passes through the throttle valve and two three-way joints and returns to the water tank to form a loop. Adjust the opening and closing degree of the throttle valve and the pressure regulating knob of the gear pump 194 so that the pressure gauge reading on the four-way valve 195 is 0.3 MPa.

[0037] Please continue to refer to Figure 3 , the sample sampling module 20 includes a second solenoid valve 21, a sample sampling plunger pump 22, a clot detection structure 23, a sampling needle 24, a third six-way valve block 250, a third solenoid valve 251, a pressure regulating valve 252, a sampling needle cleaning station 253, a cleaning liquid bottle 26, a cleaning liquid control module 27, a fourth solenoid valve 28, and a sampling needle cleaning liquid storage station 29. The second solenoid valve 21 is connected to the second six-way valve block 196, and the sample sampling plunger pump 22 is connected to the second solenoid valve 21 and the sampling needle 24. The degassed high-pressure water is supplied to the sample sampling plunger pump 22 through the second solenoid valve 21, and then supplied to the sampling needle 24 through the clot detection structure 23. Opening the second solenoid valve 21 can achieve pure water cleaning of the inner wall of the needle. The third six-way valve block 250 is connected to the first three-way joint 17. The non-degassed low-pressure water (system liquid) is supplied to the sampling needle cleaning station 253 through the third six-way valve block 250, the third solenoid valve 251, and the pressure regulating valve 252. Opening the third solenoid valve 251 can achieve pure water cleaning of the outer wall of the needle. Adjust the pressure regulating valve 252 to make the cleaning water flow stable and avoid splashing liquid. The cleaning liquid in the cleaning liquid bottle 26 is supplied to the sampling needle cleaning liquid storage station 29 through the cleaning liquid control module 27 and the fourth solenoid valve 28, and is cyclically supplied periodically through the control of the cleaning liquid control module 27. The sampling needle 24 sucks and discharges the cleaning liquid to achieve reagent cleaning of the inner wall of the needle and avoid sample cross-contamination.

[0038] Please continue to refer to Figure 4 The reagent loading module 30 includes a fifth solenoid valve 31, a reagent loading plunger pump 32, a reagent needle 33, a sixth solenoid valve 34, a reagent needle cleaning station 35, a seventh solenoid valve 36, and a reagent needle cleaning solution storage station 37. The fifth solenoid valve 31 is connected to the second six-way valve block 196, and the reagent loading plunger pump 32 is connected to the reagent needle 33. High-pressure degassed water enters the two reagent loading plunger pumps 32 through the fifth solenoid valve 31, and the two reagent loading plunger pumps 32 supply the high-pressure degassed water into the two reagent needles 33. When the fifth solenoid valve 31 is opened, pure water cleaning of the inner wall of the needle can be realized. The sixth solenoid valve 34 is connected to the third six-way valve block 250, and non-degassed low-pressure water is supplied into the reagent needle cleaning station 35 through the sixth solenoid valve 34 and a pressure regulating valve. When the sixth solenoid valve 34 is opened, pure water cleaning of the outer wall of the needle can be realized. The cleaning liquid bottle 26 is connected to the seventh solenoid valve 36 through the cleaning liquid control module 27, and the seventh solenoid valve 36 is connected to the reagent needle cleaning solution storage station 37. The cleaning liquid in the cleaning liquid bottle 26 is supplied into the reagent needle cleaning solution storage station 37 through the seventh solenoid valve 36, and periodic cyclic supply is realized through the control of the cleaning liquid control module 27. The reagent needle 33 aspirates and discharges the cleaning liquid to realize reagent cleaning of the inner wall of the needle.

[0039] Please continue to refer to Figure 5 The cleaning and separation module 40 includes an eighth solenoid valve 41, a pre-cleaning plunger pump 42, a buffer solution control module 43, a buffer solution bottle 44, a dispensing needle 45, a liquid suction needle 46, a twelfth solenoid valve 47, a first preheating device 48, and a needle group cleaning station 49. The eighth solenoid valve 41 is connected to the first six-way valve block 193, the pre-cleaning plunger pump 42 is connected to the buffer solution control module 43, and the buffer solution control module 43 includes a ninth solenoid valve 430, a tenth solenoid valve 431, and an eleventh solenoid valve 432. Non-degassed low-pressure water is supplied into the pre-cleaning plunger pump 42 through the eighth solenoid valve 41 and then supplied to the buffer solution control module 43. Among them, the ninth solenoid valve 430 and the tenth solenoid valve 431 correspond to the supply branches of the buffer solution bottle 44, and the eleventh solenoid valve 432 corresponds to the supply branch of the dispensing needle 45. There is a section of buffer pipeline indirectly connected between the two branches and the pure water branch connecting the pre-cleaning plunger pump 42. After being processed by the first preheating device 48, the temperature of the buffer solution is maintained at 28 ± 3°C. The fifteenth solenoid valve 433 connected to the buffer solution control module 43 corresponds to the liquid suction needle 46 branch for aspirating and discharging impurities in the reaction solution, and the solenoid valve at the bottom of the buffer solution control module 43 corresponds to the waste discharge branch for updating the buffer solution in the pipeline. Non-degassed low-pressure water is supplied into the needle group cleaning station 49 through the twelfth solenoid valve 47, a pressure regulating valve, and the first preheating device 48. When the twelfth solenoid valve 47 is opened, pure water cleaning of the outer wall of the needle can be realized. Adjust the pressure regulating valve to make the cleaning water flow stable and avoid splashing of the liquid.

[0040] Please continue to refer to Figure 6, the refrigeration module 50 includes a reagent bin 51 and a second centrifugal pump 52. The reagent bin 51 and the second centrifugal pump 52 are connected to the refrigeration water tank 18. The refrigeration water tank 18 stores the system liquid and cools the system liquid. The second centrifugal pump 52 extracts the cooled system liquid in the refrigeration water tank 18 to the reagent bin 51 for refrigeration and finally returns to the refrigeration water tank 18 to achieve the effect of continuous refrigeration. The circulating low-temperature water keeps the refrigeration temperature in the reagent bin 51 constant at 3-9°C to prevent the reaction reagent from deteriorating due to excessive temperature and affecting the test results. At the same time, it cooperates with the second preheating device 692 of the detection module 60 to control the temperatures of the co-reaction liquid and the cleaning liquid supplied by the detection module 60.

[0041] Please continue to refer to Figure 7 , the detection module 60 includes a thirteenth solenoid valve 61, a pipette plunger pump 62, a tube valve clamp 63, a measurement cell 64, a pipette 65, a fourteenth solenoid valve 66, a co-reaction liquid plunger pump 67, a cleaning liquid plunger pump 68, a co-reaction liquid bottle 69, a co-reaction liquid control module 691, a second preheating device 692, a co-reaction liquid buffer station 693, and a pipette cleaning station 694. The thirteenth solenoid valve 61 is connected to the first six-way valve block 193. The pipette plunger pump 62 is connected to the measurement cell 64 and the pipette 65 through the tube valve clamp 63. The degassed low-pressure water is supplied to the pipette plunger pump 62 through the thirteenth solenoid valve 61 and then divides into two paths respectively. One path is supplied to the measurement cell 64 and the pipette 65 through the tube valve clamp 63. The other path leads to waste through the tube valve clamp 63. The degassed low-pressure water is supplied to the co-reaction liquid plunger pump 67 and the cleaning liquid plunger pump 68 through the fourteenth solenoid valve 66 and then supplied to their respective flow path modules. Taking the co-reaction liquid control module 691 as an example, among them, two paths are indirectly connected with a pure water branch of the co-reaction liquid plunger pump 67 through a connection and buffer pipeline. After being processed by the second preheating device 692, the temperature of the co-reaction liquid is kept at 28±3°C. The bottom solenoid valve corresponds to the waste branch for updating the co-reaction liquid in the pipeline. The connection relationship and functions of the cleaning liquid control module 27 and the co-reaction liquid control module 691 are basically the same. The difference is that the cleaning liquid not only leads to the co-reaction liquid buffer station 693 through the solenoid valve but also leads to the sample addition pipette cleaning liquid storage station 29 and the reagent pipette cleaning liquid storage station 37 through the solenoid valve respectively. The degassed high-pressure water is supplied to the pipette cleaning station 694 through the solenoid valve and the second preheating device 629. When the solenoid valve is opened, high-pressure pure water cleaning of the outer wall of the pipette can be realized.

[0042] In the liquid path system of the full-automatic electrochemiluminescence immunoassay analyzer of the present invention, the liquid supply water tank 13 of the liquid supply module 10 is communicated with the first centrifugal pump 14. The first centrifugal pump 14 extracts the liquid in the liquid supply water tank 13. Part of the liquid extracted by the first centrifugal pump 14 forms the system liquid for cleaning. The degassing device 191 is communicated with the first centrifugal pump 14. Part of the liquid extracted by the first centrifugal pump 14 is degassed by the degassing device 191 to form low-pressure degassed water. The gear pump 194 is communicated with the degassing device 191. The gear pump 194 pressurizes part of the low-pressure degassed water to form high-pressure degassed water. The sampling needle 24 of the sample sampling module 20 is communicated with the gear pump 194. The high-pressure degassed water flows to the sampling needle 24 to clean the inner wall of the sampling needle 24. The cleaning station of the sampling needle 24 is communicated with the first centrifugal pump 14. The system liquid flows into the cleaning station of the sampling needle 24 to clean the outer wall of the sampling needle 24. The cleaning liquid bottle 26 is communicated with the cleaning liquid storage station of the sampling needle 24. The cleaning liquid in the cleaning liquid bottle 26 flows to the cleaning liquid storage station of the sampling needle 24 to clean the reagent on the inner wall of the sampling needle 24. The reagent needle 33 of the reagent sampling module 30 is communicated with the gear pump 194. The high-pressure degassed water flows to the reagent needle 33 to clean the inner wall of the reagent needle 33. The cleaning station of the reagent needle 33 is communicated with the first centrifugal pump 14. The system liquid flows into the cleaning station of the reagent needle 33 to clean the outer wall of the reagent needle 33. The cleaning liquid storage station of the reagent needle 33 is communicated with the cleaning liquid bottle 26. The cleaning liquid in the cleaning liquid bottle 26 flows to the cleaning liquid storage station of the reagent needle 33 to clean the reagent on the inner wall of the reagent needle 33. The buffer control module 43 of the cleaning and separation module 40 is communicated with the degassing device 191. The low-pressure degassed water flows to the buffer control module 43. The buffer control module 43 controls the flow of the buffer in the buffer bottle 44 to the dispensing needle 45 and the liquid suction needle 46. The needle group cleaning station 49 is communicated with the first centrifugal pump 14. The system liquid flows into the needle group cleaning station 49 to clean the outer walls of the dispensing needle 45 and the liquid suction needle 46. The measurement cell 64 of the detection module 60 is communicated with the degassing device 191. The low-pressure degassed water flows to the measurement cell 64. The aspiration needle 65 is connected to the measurement cell 64. The co-reaction liquid plunger pump 67 and the cleaning liquid plunger pump 68 are respectively communicated with the degassing device 191. The co-reaction liquid plunger pump 67 controls the supply of the co-reaction liquid in the co-reaction liquid bottle 69 to the co-reaction liquid buffer station 693. Through the above design, the liquid path system of the full-automatic electrochemiluminescence immunoassay analyzer has high precision, good reliability and high repeatability.

[0043] The above embodiments only represent several implementation manners of the present invention. The description is relatively specific and detailed, but it should not be construed as a limitation on the scope of the invention patent. It should be noted that for those of ordinary skill in the art, without departing from the concept of the present invention, several deformations and improvements can be made. These are all equivalent modifications and evolutions made to the above embodiments based on the essential technology of the present invention, and these all belong to the protection scope of the present invention.

Claims

1. The liquid path system of a fully automatic electrochemiluminescence immunoassay analyzer, comprising a liquid supply module, a sample loading module, a reagent loading module, a cleaning and separation module, and a detection module, characterized in that: The liquid supply module includes a liquid supply water tank, a first centrifugal pump, a degassing device, and a gear pump. The liquid supply water tank is connected to the first centrifugal pump. The first centrifugal pump extracts the liquid in the liquid supply water tank. Part of the liquid extracted by the first centrifugal pump forms system liquid for cleaning. The degassing device is connected to the first centrifugal pump. Part of the liquid extracted by the first centrifugal pump is degassed by the degassing device to form low-pressure degassed water. The gear pump is connected to the degassing device. The gear pump pressurizes part of the low-pressure degassed water to form high-pressure degassed water; The sample loading module includes a loading needle, a loading needle cleaning station, a cleaning solution bottle, and a loading needle cleaning solution storage station. The loading needle is connected to the gear pump. The high-pressure degassed water flows to the loading needle to clean the inner wall of the loading needle. The loading needle cleaning station is connected to the first centrifugal pump. The system liquid flows into the loading needle cleaning station to clean the outer wall of the loading needle. The cleaning solution bottle is connected to the loading needle cleaning solution storage station. The cleaning solution in the cleaning solution bottle flows to the loading needle cleaning solution storage station to clean the reagent on the inner wall of the loading needle; The reagent loading module includes a reagent needle, a reagent needle cleaning station, and a reagent needle cleaning solution storage station. The reagent needle is connected to the gear pump. The high-pressure degassed water flows to the reagent needle to clean the inner wall of the reagent needle. The reagent needle cleaning station is connected to the first centrifugal pump. The system liquid flows into the reagent needle cleaning station to clean the outer wall of the reagent needle. The reagent needle cleaning solution storage station is connected to the cleaning solution bottle. The cleaning solution in the cleaning solution bottle flows to the reagent needle cleaning solution storage station to clean the reagent on the inner wall of the reagent needle; The cleaning and separation module includes a dispensing needle, a liquid suction needle, a buffer solution bottle, a buffer solution control module, and a needle group cleaning station. The buffer solution control module is connected to the degassing device. The low-pressure degassed water flows to the buffer solution control module. The buffer solution control module controls the buffer solution in the buffer solution bottle to flow to the dispensing needle and the liquid suction needle. The needle group cleaning station is connected to the first centrifugal pump. The system liquid flows into the needle group cleaning station to clean the outer walls of the dispensing needle and the liquid suction needle; The detection module includes a measurement cell, a suction needle, a co-reactant liquid plunger pump, a co-reactant liquid bottle, and a co-reactant liquid buffer station. The measurement cell is connected to the degassing device. The low-pressure degassed water flows to the measurement cell. The suction needle is connected to the measurement cell. The co-reactant liquid plunger pump and the cleaning solution plunger pump are respectively connected to the degassing device. The co-reactant liquid plunger pump controls the co-reactant liquid bottle to supply liquid to the co-reactant liquid buffer station.

2. The liquid path system of the fully automatic electrochemiluminescence immunoassay analyzer according to claim 1, wherein: The liquid supply module further includes a five-way valve, a first throttle valve, and a pressure gauge. The five-way valve is respectively connected to the first centrifugal pump, the degassing device, the liquid supply water tank, and the pressure gauge. The liquid supply water tank, the first centrifugal pump, and the five-way valve form a loop. The first throttle valve is installed on the loop, and the opening and closing degree of the first throttle valve is adjusted to adjust the loop pressure according to the reading of the pressure gauge.

3. The liquid path system of the fully automatic electrochemiluminescence immunoassay analyzer according to claim 2, characterized in that: The liquid supply module further includes a refrigeration water tank and a first three-way joint. The first three-way joint is respectively communicated with the five-way valve, the refrigeration water tank, and the degassing device.

4. The liquid path system of the fully automatic electrochemiluminescence immunoassay analyzer according to claim 3, characterized in that: The liquid supply module further includes a second three-way joint, a first six-way valve block, a four-way valve, and a second six-way valve block. The second three-way joint is respectively connected to the output end of the degassing device, the input end of the gear pump, and the first six-way valve block. The low-pressure degassed water formed by the degassing of the degassing device flows into the first six-way valve block through the second three-way joint, and the first six-way valve block outputs the low-pressure degassed water. The low-pressure degassed water formed by the degassing of the degassing device flows into the gear pump through the second three-way joint for pressurization, and the formed high-pressure degassed water flows to the second six-way valve block through the four-way valve, and the second six-way valve block outputs the high-pressure degassed water.

5. The liquid path system of the fully automatic electrochemiluminescence immunoassay analyzer according to claim 4, characterized in that: The sample addition module further includes a sample addition plunger pump and a clot detection structure. The sample addition plunger pump is connected to the second six-way valve block. The sample addition plunger pump controls the high-pressure degassed water to flow to the reagent needle. The clot detection structure is located between the sample addition plunger pump and the reagent needle, and the clot detection structure detects the high-pressure degassed water.

6. The liquid path system of the fully automatic electrochemiluminescence immunoassay analyzer according to claim 4, characterized in that: The sample addition module further includes a third six-way valve block, a third solenoid valve, and a pressure regulating valve. The third six-way valve block is communicated with the first three-way joint. The third solenoid valve and the pressure regulating valve are installed between the third six-way valve block and the sample addition needle cleaning station.

7. The liquid path system of the fully automatic electrochemiluminescence immunoassay analyzer according to claim 4, wherein: The reagent addition module further includes a fifth solenoid valve, a reagent addition plunger pump, and a sixth solenoid valve. The reagent addition plunger pump is communicated with the second six-way valve block. The fifth solenoid valve is located between the second six-way valve block and the reagent addition plunger pump. The reagent addition plunger pump controls the cleaning of the reagent needle. The reagent needle cleaning station is communicated with the third six-way valve block. The sixth solenoid valve is located between the reagent needle cleaning station and the third six-way valve block.

8. The liquid path system of the fully automatic electrochemiluminescence immunoassay analyzer according to claim 4, characterized in that: The cleaning and separation module further includes an eighth solenoid valve and a pre-cleaning plunger pump. The pre-cleaning plunger pump is communicated with the first six-way valve block. The eighth solenoid valve is arranged between the first six-way valve block and the pre-cleaning plunger pump. The pre-cleaning plunger pump is connected to the buffer solution control module to control the buffer solution in the buffer solution bottle.

9. The liquid path system of the fully automatic electrochemiluminescence immunoassay analyzer according to claim 4, wherein: The detection module further includes a cleaning liquid plunger pump and a pipette cleaning station. The sample loading module further includes a cleaning liquid control module. The cleaning liquid plunger pump is connected to the cleaning liquid control module. The cleaning liquid control module is communicated with a cleaning liquid bottle. The cleaning liquid plunger pump controls the cleaning liquid to flow from the cleaning liquid bottle into the pipette cleaning station through the cleaning liquid control module.

10. The liquid path system of the fully automatic electrochemiluminescence immunoassay analyzer according to claim 3, wherein: The liquid path system of the automatic electrochemiluminescence immunoassay analyzer further includes a refrigeration module. The refrigeration module includes a reagent bin and a second centrifugal pump. The reagent bin and the second centrifugal pump are connected to a refrigeration water tank. The refrigeration water tank stores the system liquid and cools the system liquid. The second centrifugal pump extracts the cooled system liquid in the refrigeration water tank to the reagent bin for refrigeration.

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