Compound composition of cumic acid and eugenol, bactericide and application

Abstract

The invention provides a cumic acid and eugenol compound composition, a bactericide and application. The mass ratio of cumic acid to eugenol is (8-1): (1-4). Cumic acid and eugenol are compounded, so that tomato fusarium wilt can be prevented and treated, and fusarium wilt, phytophthora root rot, powdery mildew and the like of various crops can be prevented and treated. The binary compound agent has a remarkable synergistic effect on preventing and treating the diseases, the use amount of a single agent can be remarkably reduced, and the compound agent is expected to be developed into a new generation of green agricultural botanical fungicide.

Description

Technical Field

[0001] The present invention belongs to the field of agricultural fungicides, and specifically relates to a compound composition of cuminic acid and eugenol, a fungicide and its application. The two compounds are compounded in binary form to prevent and treat various crop diseases such as crop wilt, powdery mildew and phytophthora, and belong to a new use of fungicides and an invention of their application technology. Background Art

[0002] Crop diseases cause serious economic losses to the world's food production every year. The application of chemical fungicides is an important means of plant disease prevention and control at this stage. Due to the long-term single use of chemical fungicides, the resistance of plant pathogens to fungicides and excessive pesticide residues have become increasingly serious. In view of our country's major strategic needs for food and food safety and the increasing requirements of citizens for green food and environmental protection concepts, the development of green fungicides for crop disease prevention and control has attracted much attention. The mixed or compound use of pesticides can not only complement each other's strengths and weaknesses, expand the prevention and control spectrum, improve drug efficacy, reduce costs, reduce labor, and save costs, but more importantly, it can delay the occurrence and spread of pathogen resistance to drugs.

[0003] Cuminic acid, also known as p-isopropylbenzoic acid. As the core active ingredient in cumin seed essential oil, cuminic acid has an extremely wide range of antibacterial activity. Under in vitro experimental conditions, cuminic acid has shown extremely strong inhibitory ability against a large number of common plant pathogens such as rapeseed sclerotinia, pepper phytophthora, wilt, and rice blast, and can effectively curb the growth and reproduction of these pathogens. In potted experiments, when plants are threatened by diseases, cuminic acid can not only play a good preventive and protective role against a variety of plant diseases before the disease occurs, reducing the chance of plant infection; but also after the disease occurs, it also has an outstanding therapeutic effect, which can significantly reduce the symptoms of the plant and help the plant restore a healthy growth trend.

[0004] Eugenol, also known as 4 - allyl - 2 - methoxyphenol, is a weakly acidic phenyl terpene compound derived from herbs such as cloves, clove basil oil, cinnamon, tulips, and pepper. Its pharmacological effects are extremely extensive. In recent years, studies have shown that eugenol can damage the cell wall membrane of bacteria and induce the production of excessive reactive oxygen species to inhibit bacterial growth. Eugenol has strong abilities to scavenge oxygen free radicals and reduce iron, can inhibit lipid and DNA oxidative damage, has protective effects on the myocardium, mucosa, and nerves, can induce apoptosis of cancer - associated fibroblasts, and inhibit cancer cell invasion and angiogenesis in cancer tissues. Eugenol is widely used in the treatment of various diseases such as diabetes, viral diseases, fungal diseases, non - alcoholic fatty liver disease, and Alzheimer's disease. As a natural plant extract, it has extensive effects and few adverse reactions, and has broad prospects for development and application. Currently, research on eugenol mainly focuses on fields such as food preservation, plant protection, beauty, stomatology, and animal husbandry and is still in its initial stage. The related mechanisms of the known pharmacological effects of eugenol have not been fully elucidated. This study reviews the advantages of eugenol in anti - inflammation, regulation of energy metabolism, antioxidant, myocardial protection, mucosal protection, nerve protection, and anti - tumor and the research progress of its action mechanisms, in order to provide reference for the further development and clinical application of eugenol. Summary of the Invention

[0005] The object of the present invention is to provide a compound composition of cuminic acid and eugenol, a fungicide, and its application. The binary compounding of cuminic acid and the natural product eugenol can be used simultaneously to control various crop diseases such as crop fusarium wilt, powdery mildew, and Phytophthora blight.

[0006] To achieve the above object, the technical solutions adopted by the present invention include:

[0007] A compound composition of cuminic acid and eugenol, calculated by mass ratio, cuminic acid:eugenol=(8 - 1):(1 - 4).

[0008] Optionally, cuminic acid:eugenol=(3 - 1):(1 - 2).

[0009] Optionally, cuminic acid:eugenol = 4:1.

[0010] The application of the compound composition of cuminic acid and eugenol according to any one of the present invention in the preparation of a plant fungicide.

[0011] Optionally, calculated by mass percentage, in the fungicide, cuminic acid is 0.5% - 35%, and eugenol is 0.5% - 35%.

[0012] Optionally, the plant fungicide is used to control crop fusarium wilt, Phytophthora blight, and / or powdery mildew.

[0013] A plant fungicide, wherein the active ingredient of the plant fungicide is a compound composition of cuminic acid and eugenol according to any one of the present invention;

[0014] By mass percentage, in the fungicide, cuminic acid is 0.5% - 35%, and eugenol is 0.5% - 35%.

[0015] Optionally, the fungicide is an emulsion in water, and the mass percentage composition is:

[0016] Cuminic acid 0.5% - 35%, eugenol 0.5% - 35%, emulsifier 5% - 20%, dispersant 1% - 5%, antifreeze 0.5% - 4%, preservative 0.5% - 4%, solvent 5% - 20%, and water is made up to 100%;

[0017] The dispersant is polyvinyl alcohol, MNO, gum arabic, xanthan gum or magnesium aluminum silicate;

[0018] The solvent is at least one of methanol, N,N - dimethylformamide, ethyl acetate, cyclohexanone, furfural, 5 - methylfurfural, ethanol and ethyl acetate;

[0019] The antifreeze is sodium nitrite, calcium chloride, ethylene glycol, propylene glycol, glycerol or urea;

[0020] The preservative is potassium sorbate, sodium benzoate, benzoic acid or benzaldehyde;

[0021] The emulsifier is at least one of 602, agricultural emulsion 500, NP - 10, fatty acid polyoxyethylene ester, TX - 10, 4103, OP - 10, Well205 and M30.

[0022] Optionally, the fungicide is a soluble liquid formulation, and the mass percentage composition is:

[0023] Cuminic acid 0.5% - 35%, eugenol 0.5% - 35%, emulsifier 5% - 20%, antifreeze 0.5% - 4%, and solvent is made up to 100%;

[0024] The emulsifier is at least one of OP - 10, 2201, agricultural emulsion 500, agricultural emulsion 600, agricultural emulsion BY, agricultural emulsion EL and NP - 30;

[0025] The solvent is at least one of methanol, ethanol, cyclohexanone, ethyl acetate, N,N - dimethylacetamide, N,N - dimethylformamide, dimethyl sulfoxide, sec - butyl acetate, furfural and 5 - methylfurfural;

[0026] The antifreeze is sodium nitrite, propylene glycol, glycerol or urea.

[0027] Compared with the prior art, the advantages of the present invention are:

[0028] (1) Through a series of proportion studies, the present invention finds that when cymenic acid and eugenol are proportioned according to a mass ratio of 8:1 to 1:4, the synergistic coefficient is greater than 1.5, and both have obvious synergistic effects.

[0029] (2) The binary compound agent has significant synergistic effects in controlling the above-mentioned diseases, and can significantly reduce the usage amount of a single agent, and is expected to be developed into a new generation of green agricultural plant-derived fungicides.

[0030] (3) At present, there is only 1 agent registered on the Chinese Pesticide Information Network for controlling tomato wilt, and the agents for controlling tomato wilt are scarce. This compound preparation has a good control effect on tomato wilt and has great potential for being registered as an agent for controlling tomato wilt. Detailed implementation manners

[0031] In order to more clearly present the purpose, technical solution and its advantages of the present invention, the present invention will be described in more detail below in conjunction with specific embodiments. It should be clear that the embodiments described here are only part of the embodiments of the present invention, not all of them. All other embodiments obtained by those of ordinary skill in the art based on the embodiments provided by the present invention without creative work are covered by the protection scope of the present invention.

[0032] The compound fungicide of cymenic acid and eugenol of the present invention is a preparation prepared by compounding cymenic acid and eugenol according to a mass ratio of 8:1 to 1:4. It can not only control tomato wilt, but also be used to control powdery mildew and Phytophthora blight of various crops. The binary compound agent has significant synergistic effects in controlling the above-mentioned diseases, and can significantly reduce the usage amount of a single agent. This compound preparation is expected to be developed into a new generation of green agricultural plant-derived fungicides.

[0033] For example, the dosage forms of the fungicide include emulsion in water and soluble liquid agent;

[0034] The components and weight percentages of the emulsion in water are as follows: cuminaldehyde 0.5% - 35%, eugenol 0.5% - 35%, emulsifier 5% - 20%, dispersant 1% - 5%, antifreeze 0.5% - 4%, preservative 0.5% - 4%, solvent 5% - 20%, and the solvent is made up to 100%; polyvinyl alcohol, gum arabic, xanthan gum or magnesium aluminum silicate polyvinyl alcohol, MNO, gum arabic, xanthan gum or magnesium aluminum silicate is the dispersant; one or more of methanol, N,N-dimethylformamide, ethyl acetate, cyclohexanone, furfural, 5-methylfurfural, ethanol and ethyl acetate is the solvent; sodium nitrite, calcium chloride, ethylene glycol, propylene glycol, glycerol or urea is the antifreeze; potassium sorbate, sodium benzoate, benzoic acid or benzaldehyde is the preservative; one or a mixture of two or more of agricultural emulsion 500, NP-10, fatty acid polyoxyethylene ester, TX-10, 4103, OP-10, Well205 and M30 is the emulsifier.

[0035] The components and weight percentages of the soluble concentrate are: cuminaldehyde 0.5% - 35%, eugenol 0.5% - 35%, emulsifier 5% - 20%, antifreeze 0.5% - 4%, and the solvent is made up to 100%; one or more of OP-10, 2201, agricultural emulsion 500, agricultural emulsion 600, agricultural emulsion BY, agricultural emulsion EL and NP-30 is the emulsifier; one or more of methanol, ethanol, cyclohexanone, ethyl acetate, N,N-dimethylacetamide, N,N-dimethylformamide, dimethyl sulfoxide, sec-butyl acetate, furfural and 5-methylfurfural is the solvent; one or more of sodium nitrite, propylene glycol, glycerol or urea is the antifreeze.

[0036] Example 1: Toxicity of cuminaldehyde and eugenol against Fusarium oxysporum f. sp. lycopersici

[0037] To accurately determine the toxicity of cuminaldehyde and eugenol against the pathogen, the mycelial growth rate method was used in this study. The specific operation is as follows: First, the original drugs of cuminaldehyde and eugenol were respectively dissolved in methanol as the solvent to prepare a series of mother liquors with different concentrations. Subsequently, a pipette was used to accurately measure the above mother liquors and add them to the PDA medium, and mix well to prepare a medium containing drugs with different concentrations.

[0038] From the edge of the cultured pathogen colony, a mycelial disc with a diameter of 0.5 cm was selected and carefully placed in the center of the above-prepared drug-containing medium. The inoculated petri dishes were uniformly placed in an incubator with a set temperature of 25°C for constant-temperature culture. During the culture process, the diameter of the colony was regularly measured by the cross method, and the inhibition rate was calculated through the measured data. Finally, the obtained data was analyzed using DPS software to calculate the effective median inhibitory concentration (EC 50 ).

[0039] Table 1 Toxicity determination of different ratios of cuminic acid and eugenol against Fusarium oxysporum f. sp. lycopersici

[0040]

[0041] As can be seen from Table 1, the EC 50 values of cuminic acid and eugenol against Fusarium oxysporum f. sp. lycopersici were 45.091 μg / mL and 65.491 μg / mL respectively, indicating that both cuminic acid and eugenol have good antibacterial activities against Fusarium oxysporum f. sp. lycopersici.

[0042] Example 2: Synergistic effect of different ratios of cuminic acid and eugenol

[0043] The toxicity of cuminic acid and eugenol against pathogenic bacteria at different ratios was determined. In this study, the experiment was carried out by referring to the mycelial growth rate method. The specific process is as follows: The toxicity of cuminic acid and eugenol at different ratios against pathogenic bacteria was determined by referring to the mycelial growth rate method.

[0044] 1. Preparation of stock solutions: The original drugs of cuminic acid and eugenol were respectively dissolved in methanol as solvents to prepare stock solutions with the same concentration.

[0045] 2. Ratio mixing: According to the experimental design, the above two stock solutions were mixed in different volume ratios to obtain compound solutions with different ratios.

[0046] 3. Preparation of drug-containing medium: Referring to the operation method of Example 1, a pipette gun was used to accurately measure compound solutions with different ratios and add them to the PDA medium, and stirred evenly to prepare a series of drug-containing media with different concentration gradients.

[0047] 4. Inoculation and culture: A mycelial disc with a diameter of about 0.5 cm was selected from the edge of the well-cultured pathogenic bacteria colony and carefully transferred to the center of the PDA plate containing compound agents with different concentrations. After inoculation, the plate was placed in a constant temperature incubator at 25 °C for 2 days of constant temperature culture.

[0048] 5. Data measurement and calculation: When the colony diameter of the blank control group grew to more than 2 / 3 of the diameter of the entire culture dish, the cross method was used to measure the colony diameter of all groups. Each treatment was set with 3 replicates to ensure the accuracy of the data. According to the measured data, the mycelial growth inhibition rate of each treatment group was calculated. Finally, the DPS software was used to analyze the experimental data, and then the effective inhibitory median concentration (EC50) was calculated.

[0049] According to the Wadley method, the synergistic ratio (SR) of the compound agent is calculated using the following formula. When SR is greater than 1.5, it is a synergistic effect; when SR is between 0.5 and 1.5, it is an additive effect; when SR is less than 0.5, it is an antagonistic effect (a and b are the proportions of cuminic acid and eugenol in the compound, EC 50 (A) and EC 50 (B) are the effective median concentrations of cuminic acid and eugenol, EC 50 (Exp) is the theoretical inhibitory median concentration, and EC 50 (Obs) is the actually measured inhibitory median concentration).

[0050]

[0051] Table 2 Toxicity determination of different ratios of cuminic acid and eugenol against Fusarium oxysporum f. sp. lycopersici

[0052]

[0053]

[0054] Based on the data in Table 2, it is clearly visible that the toxicity of cuminic acid and eugenol against Fusarium oxysporum f. sp. lycopersici shows significant differences due to the different ratios of the two. After calculation, the synergistic ratio is in the range of 1.057 to 1.979. It is worth noting that for all the ratios involved in the experiment, the synergistic ratio is higher than 0.5. This result strongly indicates that under the various ratio conditions set in this experiment, the compound use of cuminic acid and eugenol shows an additive effect. Especially when the ratio of cuminic acid and eugenol is between 8:1 and 1:4, the synergistic ratio is greater than 1.5. This data intuitively shows that under the above specific ratios, the combined effect of cuminic acid and eugenol against Fusarium oxysporum f. sp. lycopersici is not only a simple addition, but shows a very obvious synergistic effect, which can more effectively inhibit the growth and reproduction of Fusarium oxysporum f. sp. lycopersici compared with the single use of cuminic acid or eugenol, providing a valuable reference basis for subsequent related applications and research.

[0055] Example 3: Production of 20% cuminic acid and 5% eugenol composition emulsion in water

[0056] Accurately weigh 245 kg of 98% cuminic acid, and then weigh 61 kg of 98% eugenol. Mix the two active ingredients in 2M 3Mix well in the blending tank. Weigh accurately 15 kg of sodium benzoate, put it into the blending tank, and mix well again. Then accurately add 95 kg of emulsifier OP-10 into the blending tank. After that, add 10 kg of gum arabic, 15 kg of calcium chloride, and 100 kg of N,N-dimethylformamide respectively, and add water to make up to 1000 kg. Mix well in the blending tank. Stir continuously for 1 hour. A water emulsion product of 1 ton of cymeneic acid and eugenol composition is prepared.

[0057] Sampling and chemical analysis shows that the content of cymeneic acid is 20% and the content of eugenol is 5%. Pack the product into 500 mL double-barrier glass bottles for encapsulation, seal the bottle mouth, and label it as a water emulsion product of the composition with a content of 25%.

[0058] Example 4: Production of 20% cymeneic acid and 5% eugenol composition soluble concentrate

[0059] Accurately weigh 245 kg of 98% cymeneic acid quantitatively, and then weigh 61 kg of 98% eugenol. Mix the two active ingredients well in a 2M 3 blending tank. Slowly add the pre-mixed active ingredient mixture to 524 kg of ethanol solvent, and keep stirring continuously until the active ingredients are completely dissolved to form a uniform and transparent solution. Then slowly add 150 kg of OP-10 emulsifier to the solution in which the active ingredients are completely dissolved. Add 20 kg of antifreeze sodium nitrite during the addition process. Then keep stirring to make it evenly dispersed. After the emulsifier is added, gradually increase the stirring speed to high speed and carry out an emulsification operation for 30 minutes. A stable emulsification system is formed by high-speed stirring, and the active ingredients are evenly dispersed in the solvent to obtain a soluble concentrate with good stability and fluidity. A soluble concentrate product of 1 ton of cymeneic acid and eugenol composition is prepared.

[0060] Sampling and chemical analysis shows that the content of cymeneic acid is 20% and the content of eugenol is 5%. Pack the product into 500 mL double-barrier glass bottles for encapsulation, seal the bottle mouth, and label it as a soluble concentrate product of the composition with a content of 25%.

[0061] Example 5: Control efficacy of 25% water emulsion and soluble concentrate of cymeneic acid and eugenol mixture against tomato fusarium wilt and pepper phytophthora blight

[0062] The control efficacy of 25% water emulsion and soluble concentrate against tomato fusarium wilt and pepper phytophthora blight was determined by greenhouse pot experiments. Three concentrations were set for the test agents respectively. Bacillus amyloliquefaciens water dispersible granules were used as the positive control agent for controlling tomato fusarium wilt, and 5% cymeneic acid aqueous solution was used as the positive control agent for controlling tomato fusarium wilt. Each treatment had 5 replicates. The inoculation of tomato fusarium wilt and the investigation of disease index were carried out with reference to the literature reported by Luo Man et al.

[0063] Table 3 Control efficacy of the compound agent of cuminic acid and eugenol against tomato Fusarium wilt

[0064]

[0065] Note: According to Fisher's least significant difference test, values with different letters in the same column indicate significant differences between the values (P = 0.05).

[0066] Based on the data in Table 3, the results of greenhouse pot experiments showed that the aqueous emulsion and soluble concentrate of the compound of cuminic acid and eugenol exhibited good control efficacy against tomato Fusarium wilt. In terms of concentration, when the dosage of the active ingredient of the compound preparation was 100 μg / mL, its control efficacy against tomato Fusarium wilt was not significantly different from that of the control agent. However, when the dosage was increased to 200 μg / mL, the control efficacy of the compound preparation against tomato Fusarium wilt was significantly better than that of the control agent. From the perspective of dosage form, under the same dosage conditions, the control efficacy of the soluble concentrate in the compound preparation was slightly better than that of the aqueous emulsion. This result provides an important basis for the selection of concentration and dosage form considerations in the actual control of tomato Fusarium wilt by this compound preparation in the future.

[0067] In the study on the control efficacy against Phytophthora capsici, pepper plants at the seedling stage before the post-flowering stage were selected. With a Phytophthora capsici spore suspension at a concentration of 5×10 6 cfu / mL, it was evenly sprayed on the pepper leaves with a knapsack sprayer. After 48 hours of inoculation, the corresponding agents were sprayed on different treatment groups with a knapsack sprayer again. 6 pepper plants were selected for each treatment, and 3 replicates were set. 5 to 7 days after inoculation, 10 leaves were collected from top to bottom for each pepper plant, and the disease incidence of the leaves was investigated and recorded strictly according to the disease grading standard of GB / T 17980.28-2000 to evaluate the control efficacy of different agents against Phytophthora capsici.

[0068] Table 4 Control efficacy of the compound agent of cuminic acid and eugenol against Phytophthora capsici

[0069]

[0070] Note: According to Fisher's least significant difference test, values with different letters in the same column indicate significant differences between the values (P = 0.05). Control efficacy (%) = (control lesion diameter - treated lesion diameter) / (control lesion diameter) * 100.

[0071] Based on the data in Table 4, it is clearly visible that the compound preparation of cuminic acid and eugenol is significantly effective in controlling Phytophthora capsici. When the dosage of the compound preparation is 50 μg / mL, its control effect has exceeded 50%, showing good disease inhibition ability. As the concentration increases, the advantage becomes more obvious. At a dosage of 100 μg / mL, the control effect of the compound preparation is better than that of the control agent at the same dosage; when the concentration reaches 200 μg / mL, the control effect of the compound preparation is significantly higher than that of the control agent. From the perspective of dosage form differences, under the same dosage conditions, the control effect of the soluble liquid formulation in the compound preparation is slightly higher than that of the emulsion in water.

[0072] Example 6: Control Effect of 20% Emulsion in Water and Soluble Liquid Formulation of the Compound of Cuminic Acid and Eugenol on Powdery Mildew of Wheat

[0073] A study on the control effect of 20% emulsion in water and soluble liquid formulation of the compound on powdery mildew of wheat was carried out. The tested agents were set at three concentrations of 100, 200, and 400 μg / mL. 12.5% diniconazole emulsifiable concentrate was selected as the positive control agent for controlling powdery mildew of wheat. Four replicates were set for each concentration treatment, and 50 leaves were randomly investigated for each replicate, with a total of 200 leaves investigated.

[0074] Table 5 Control Effect of the Compound of Cuminic Acid and Eugenol on Powdery Mildew of Wheat

[0075]

[0076] Note: According to Fisher's least significant difference test, values with different letters marked after the values in the same column indicate significant differences between the values (P = 0.05).

[0077] It can be clearly seen from the data in Table 5 that the compound preparation of cuminic acid and eugenol shows good effects in controlling powdery mildew of wheat. When the dosage of the compound preparation is 100 μg / mL, the control effects of different dosage forms are all above 55%; when the dosage is increased to 200 μg / mL, the control effect is further improved, all exceeding 75%. When the concentration of the compound preparation reaches 400 μg / mL, its control effect is more significant, all reaching above 85%, and at this time, there is no significant difference compared with the control effect of the control agent. In terms of dosage form comparison, under the same dosage conditions, the control effects of the soluble liquid formulation and the emulsion in water of the compound preparation are basically the same, without showing obvious regular differences.

[0078] Although the present invention has been described in detail with general descriptions and specific embodiments above, based on the present invention, some modifications or improvements can be made, which are obvious to those skilled in the art. Therefore, these modifications or improvements made without departing from the spirit of the present invention all fall within the scope of protection required by the present invention.

Claims

1. A compound composition of cumic acid and eugenol, characterized in that, By mass ratio, cuminaldehyde: eugenol = (8 - 1):(1 - 4).

2. The compound composition of cumic acid and eugenol according to claim 1, wherein Cuminaldehyde: eugenol = (3 - 1):(1 - 2).

3. The compound composition of cumic acid and eugenol according to claim 1, characterized in that, Cuminaldehyde: eugenol = 4:

1.

4. Use of the compound composition of cuminaldehyde and eugenol according to any one of claims 1 - 3 for preparing a plant fungicide.

5. The application according to claim 4, wherein By mass percentage, in the fungicide, cuminaldehyde is 0.5% - 35%, and eugenol is 0.5% - 35%.

6. The application according to claim 4, characterized in that, The plant fungicide is used for preventing and treating tomato fusarium wilt, pepper phytophthora blight and / or wheat powdery mildew.

7. A plant fungicide, characterized in that, The active ingredient of the plant fungicide is the compound composition of cuminaldehyde and eugenol according to any one of claims 1 - 3; By mass percentage, in the fungicide, cuminaldehyde is 0.5% - 35%, and eugenol is 0.5% - 35%.

8. The plant fungicide according to claim 7, characterized in that, The fungicide is an emulsion in water, and the mass percentage composition is: Cuminaldehyde 0.5% - 35%, eugenol 0.5% - 35%, emulsifier 5% - 20%, dispersant 1% - 5%, antifreeze 0.5% - 4%, preservative 0.5% - 4%, solvent 5% - 20%, water is added to make up 100%; The dispersant is polyvinyl alcohol, MNO, arabic gum, xanthan gum or magnesium aluminum silicate; The solvent is at least one of methanol, N,N - dimethylformamide, ethyl acetate, cyclohexanone, furfural, 5 - methylfurfural, ethanol and ethyl acetate; The antifreeze is sodium nitrite, calcium chloride, ethylene glycol, propylene glycol, glycerol or urea; The preservative is potassium sorbate, sodium benzoate, benzoic acid or benzaldehyde; The emulsifier is at least one of 602, agricultural emulsion 500, NP - 10, fatty acid polyoxyethylene ester, TX - 10, 4103, OP - 10, Well205 and M30.

9. The plant fungicide according to claim 7, characterized in that, The fungicide is a soluble liquid agent, and the mass percentage composition is: Cuminaldehyde 0.5% - 35%, eugenol 0.5% - 35%, emulsifier 5% - 20%, antifreeze 0.5% - 4%, solvent is added to make up 100%; The emulsifier is at least one of OP - 10, 2201, agricultural emulsion 500, agricultural emulsion 600, agricultural emulsion BY, agricultural emulsion EL and NP - 30; The solvent is at least one of methanol, ethanol, cyclohexanone, ethyl acetate, N,N - dimethylacetamide, N,N - dimethylformamide, dimethyl sulfoxide, sec - butyl acetate, furfural and 5 - methylfurfural; The antifreeze is sodium nitrite, propylene glycol, glycerol or urea.

Images