An oil control composition and uses thereof, cosmetic

Abstract

The application belongs to the technical field of cosmetics, and discloses an oil control composition, application and cosmetics thereof. The oil control composition is obtained by water extraction of a plant combination of bitter almond seeds, reed, radix ophiopogonis and kaempferia galanga roots. The weight ratio of the bitter almond seeds, reed, radix ophiopogonis and kaempferia galanga roots in the plant combination is 3-7:4-8:3-7:1-6. When the four plant extracts are used together, a synergistic effect can be achieved, the skin is acted on through multiple pathways, the synthesis and secretion of lipids in sebaceous gland cells are reduced, and the skin oil is decomposed. The oil control composition has a high 5alpha-reductase inhibition rate, and has an excellent oil control effect.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of cosmetics, and specifically discloses an oil control composition, use thereof, and a cosmetic. BACKGROUND

[0002] In modern society, people pay more and more attention to skin health and appearance. People with oily skin are faced with many troubles. Excessive oil secretion not only makes the skin look shiny, affecting the appearance, but also easily causes skin problems such as pore blockage, acne, and comedones, which negatively affect personal image and mental health. At the same time, the skin is prone to appear uncomfortable symptoms such as sensitivity, redness, and itching under external stimulation such as ultraviolet rays, environmental pollution, and improper skin care. At present, there are many oil control products and technologies on the market. Traditional oil control products mostly use adsorbents such as talc and kaolin, which reduce the oil on the skin surface through physical adsorption. However, this method only temporarily solves the surface problem and cannot regulate oil secretion from the root, resulting in poor oil control effect. Moreover, long-term use may cause burden to the skin, affecting the normal respiration and metabolism of the skin. Chemical synthetic oil control ingredients can regulate oil secretion to some extent, but may have irritant properties and easily cause skin allergic reactions, which are not suitable for sensitive skin.

[0003] Therefore, the technical problem to be solved by the present application is how to prepare an oil control product with high oil control efficiency. SUMMARY

[0004] The present application aims to provide an oil control composition containing bitter almond seed extract, reed extract, ophiopogon root extract, and kaempferia galanga root extract. Through the synergistic effect of the four, oil secretion can be effectively reduced to achieve the purpose of oil control.

[0005] Meanwhile, the present application also provides the use of the oil control composition and a cosmetic.

[0006] To achieve the above-mentioned purpose, the present application provides the following technical solutions.

[0007] An oil control composition is obtained by water extraction of a plant combination of bitter almond seeds, reed, ophiopogon root, and kaempferia galanga root. The weight ratio of bitter almond seeds, reed, ophiopogon root, and kaempferia galanga root in the plant combination is 3-7:4-8:3-7:1-6.

[0008] In the present application, the efficacy of each component is as follows:

[0009] Prunus amygdalus dulcis seed extract: Prunus amygdalus dulcis seed extract is rich in vitamins, fatty acids and minerals, and can help regulate the activity of sebaceous glands by supplementing the nutrients of the skin lipid barrier. Its moisturizing properties can reduce the phenomenon of excessive secretion of oil due to skin water loss, thereby indirectly achieving oil control effect;

[0010] Phragmites communis extract: flavonoids and other components in Phragmites communis extract have a plant estrogen-like effect, can bind to hormone receptors in human skin cells, regulate the balance of hormones in the body, and reduce the sensitivity of sebaceous glands to androgens, thereby reducing the secretion of oil.

[0011] Ophiopogon japonicus root extract: high isoflavones and other components in Ophiopogon japonicus root extract can act on sebaceous gland cells. By affecting the signal transduction pathway in the cells, the expression of genes related to the synthesis and secretion of lipids in sebaceous gland cells is reduced, thereby reducing the synthesis and secretion capacity of sebaceous glands, controlling the production of skin oil from the source, keeping the secretion of skin oil at a normal level, and achieving oil control effect.

[0012] Kaempferia galanga root extract: Kaempferia galanga root extract has a certain inhibitory effect on some common skin microorganisms such as Propionibacterium acnes and Malassezia. When these microorganisms overproliferate on the skin surface, they can decompose the oil on the skin surface, produce irritating substances, cause skin inflammation, and further lead to abnormal secretion of oil. Kaempferia galanga root extract can maintain the balance of skin microecology by inhibiting the growth and reproduction of these microorganisms, reduce skin inflammation and abnormal oil secretion caused by microbial infection, and make the skin more refreshing and comfortable.

[0013] In the research of the present application, it is found that the four plant extracts described above can play a synergistic effect when used together. They can act on the skin through multiple pathways, reduce the synthesis and secretion of lipids in sebaceous gland cells, and decompose skin oil, thereby achieving excellent oil control effect.

[0014] Preferably, the extraction method of the extract is as follows:

[0015] Step 1: The plant combination of Prunus amygdalus dulcis, Phragmites communis, Ophiopogon japonicus root and Kaempferia galanga root is crushed through a 40-mesh sieve, then added to water and stirred at 65±5℃ for 2-4h, and the precipitate is removed by centrifugation. The centrifugal liquid is concentrated under reduced pressure at 60±5℃ and -0.08±0.01MPa to 25-35wt% of the mass of the centrifugal liquid, to obtain a first concentrated liquid, wherein the weight ratio of the plant combination to water is 1-3:8-15;

[0016] Step 2: add equal volume of 95vol% ethanol into the first concentrated solution, and then alcoholize for 12-24h, centrifugalize to remove the precipitate, and then concentrate the centrifugal solution to be alcohol-free under reduced pressure at 60±5℃ and -0.08±0.01MPa, to obtain a second concentrated solution; add butanediol and water into the second concentrated solution, and then stir to be uniform, and then perform ultrafiltration under 0.25±0.1MPa, to obtain an ultrafiltration solution, wherein the weight ratio of the second concentrated solution, butanediol and water is 1-3:3-5:5-7;

[0017] Step 3: add a preservative into the ultrafiltration solution, and then sterilize at 85±3℃ for 0.5-1h, to obtain the oil-control composition.

[0018] In addition, the present application also provides the use of the above oil-control composition in the preparation of cosmetics.

[0019] Preferably, the cosmetics have oil-control effect.

[0020] Finally, the present application also discloses a cosmetic containing the oil-control composition as described above.

[0021] Preferably, the cosmetics contain 0.01-30wt% of the oil-control composition.

[0022] More preferably, the dosage form of the cosmetics is emulsion, cream, essence, toner, shampoo, cleansing mousse, facial cleanser or hair conditioner.

[0023] Compared with the prior art, the present application has the following advantages:

[0024] The oil-control composition of the present application is obtained by combining the extract of bitter almond seed, the extract of reed, the extract of ophiopogon root and the extract of kaempferia galanga root, and when the four kinds of plant extracts are used together, they have the synergistic effect of improving the oil-control effect. BRIEF DESCRIPTION OF DRAWINGS

[0025] Figure 1 The figure is the observation of the chicken embryo before the sample of Example 1 is used;

[0026] Figure 2 The figure is the observation of the chicken embryo after the sample of Example 1 is used. DETAILED DESCRIPTION

[0027] The technical solutions in the embodiments of the present application will be described clearly and completely below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all the other embodiments obtained by those skilled in the art without creative work fall within the protection scope of the present application.

[0028] Product information:

[0029] Bitter almond seed: water content 4.8wt%, purchased from Bozhou Pocket Doctor Biotechnology Co., Ltd.;

[0030] Phragmites: water content 6.1wt%, purchased from Bozhou Source Upland Pharmaceutical Co., Ltd.;

[0031] Ophiopogon japonicus root: water content 13.5wt%, purchased from Bozhou Pocket Doctor Biotechnology Co., Ltd.;

[0032] Bark of the mountain: water content 9.3wt%, purchased from Bozhou Pocket Doctor Biotechnology Co., Ltd.

[0033] The preparation method of the oil control composition of each example and comparative example is as follows:

[0034] Step 1: After the plant combination of bitter almond seed, phragmites, ophiopogon japonicus root and bark of the mountain was crushed through a 40 mesh sieve, it was added to water at 65℃ and stirred for 3h. The precipitate was removed by centrifugation, and the centrifugal liquid was concentrated under reduced pressure at 60℃ and-0.08MPa to 30wt% of the mass of the centrifugal liquid. The first concentrated liquid was obtained, wherein the weight ratio of the plant combination to water was 2:10.

[0035] Step 2: An equal volume of 95vol% ethanol was added to the first concentrated liquid, and alcohol precipitation was carried out for 18h. The precipitate was removed by centrifugation, and the centrifugal liquid was concentrated under reduced pressure at 60℃ and-0.08MPa until no alcohol was present. The second concentrated liquid was obtained. Butanediol and water were added to the second concentrated liquid and stirred uniformly. Ultrafiltration was carried out at 0.25±0.1MPa to obtain an ultrafiltrate, wherein the weight ratio of the second concentrated liquid, butanediol and water was 2:3:5.

[0036] Step 3: 1,2-hexanediol equivalent to 0.5% of the weight of the ultrafiltrate and p-hydroxyacetophenone 0.5% were added to the ultrafiltrate. Sterilization was carried out at 85℃ for 1h to obtain the oil control composition.

[0037] The formula of the plant combination of each example and comparative example is shown in Table 1;

[0038] Table 1 Formula of plant combination (weight parts)

[0039] Bitter almond seed Reed Ophiopogon root Kaempferia root Example 1 5 6 5 4 Example 2 3 8 3 6 Example 3 7 4 7 1 Comparative Example 1 0 6 5 9 Comparative Example 2 9 6 5 0 Comparative Example 3 0 10.5 9.5 0

[0040] Performance test

[0041] Chicken embryo chorioallantoic membrane test

[0042] 1. Purpose and principle of the test

[0043] The chicken embryo chorioallantoic membrane test is an in vitro evaluation method of eye irritation which was used early. The chorioallantoic membrane (CAM) is a respiratory membrane that surrounds the chicken embryo. This test takes advantage of the characteristics of the chorioallantoic membrane of the incubated chicken embryo, which is complete, clear and transparent in the middle of the vascular system. A certain amount of test substance is directly contacted with the chorioallantoic membrane of the chicken embryo, and after a period of action, the changes in the toxicity effect indicators (such as bleeding, coagulation and vascular fusion) of the chorioallantoic membrane are observed. These indicators reflect the changes in the morphological structure, color and permeability of the blood vessels and vascular network, as well as the denaturation of chorioallantoic membrane proteins and the degree of damage. Then a score is obtained to evaluate the eye irritation of the test substance.

[0044] The purpose of this test is to test the ability of the test substance to cause toxicity changes in the chorioallantoic membrane of the chicken embryo, and to evaluate the elements and processes of the potential eye irritation of the evaluated substance.

[0045] 2. Test materials and methods

[0046] 2.1 Instruments and equipment

[0047] Full-automatic incubator;

[0048] Stereo microscope;

[0049] SPF chicken embryos.

[0050] 2.2 Reagents

[0051] Sodium chloride;

[0052] Sodium dodecyl sulfate (SDS).

[0053] 2.3 Incubation conditions

[0054] Room temperature 20℃~25℃, relative humidity 45%~70%. Incubation temperature 37.5℃±0.5℃, relative humidity 55%~70%, rotation 3 times / h~6 times / h. 9-day-old chicken embryos do not need to be rotated during incubation.

[0055] 2.4 Test method

[0056] (1) Test operation steps

[0057] This test selected 6 embryos for each group, and recorded the condition of the chorioallantoic membrane with a photographic device. The composition of Example 1 was diluted with pure water to form a 2% aqueous solution, which was added to the chorioallantoic membrane of the chicken embryo. The time of adding the sample was recorded and a wet plastic wrap was placed on the gas chamber. The chicken embryo was moved to a constant temperature and humidity incubator for cultivation. The degree of change in each toxicity effect was observed and recorded by taking pictures.

[0058] (2) Results observation

[0059] Observe and record the bleeding, coagulation and vascular fusion performance, and score according to the severity.

[0060] (3) Data analysis

[0061] Table 2 Score criteria

[0062]

[0063]

[0064] The test was performed using the endpoint evaluation method, and the endpoint evaluation (ES) was calculated, with the result being rounded to two decimal places; ES≤4, no irritation; 4<ES≤12, mild irritation; 12<ES<16, moderate irritation; ES≥16, strong irritation / corrosion; score per embryo = the sum of the degree of observed bleeding, coagulation and vasolysis per embryo; ES = the average value of the mathematical total of 6 embryos.

[0065] Upon testing, the composition of Example 1 had an ES = 2.67, indicating that the sample was mild and non-irritating, while the reference Figure 1 、 Figure 2 A comparison of the state of the chicken embryos before and after the drop of the aqueous solution of the composition of Example 1 to the chicken embryos, wherein, Figure 1 is a view of the chicken embryos before use of the sample, Figure 2 is a view of the chicken embryos after use of the sample.

[0066] 5α-reductase inhibition rate

[0067] 1. Purpose and principle of the test

[0068] The sebaceous glands of oily skin have a relatively strong secretory function, and the face feels greasy and is not easy to clean, with an oily appearance that affects the appearance. At the same time, some corresponding skin diseases, such as acne vulgaris and seborrheic dermatitis, are also prone to occur. In view of the relationship between 5α-reductase and sebaceous glands, the inhibitory effect of the test sample on 5α-reductase is usually used to reflect the effect of sebum regulation, so as to characterize the oil control effect of the test sample. In this test, the inhibitory rate of 5α-reductase of the test sample and the negative control was compared according to the laboratory method, and if the inhibitory rate of the test sample was higher than that of the negative control, it was considered that the test sample had a certain oil control effect.

[0069] 2. Test materials and methods

[0070] 2.1 Instruments and equipment

[0071] BSA224S analytical balance;

[0072] Agilent 1220 high-performance liquid chromatograph;

[0073] 2.2 Reagents

[0074] Testosterone, concentration 98%;

[0075] NADPH, concentration of 90%;

[0076] Methanol, purity of HPLC grade;

[0077] Buffer: Tris-HCl.

[0078] 2.3 Test method

[0079] (1) Treatment of control and test sample

[0080] Sample group: dilute the compositions of Examples 1-3 and Comparative Examples 1-3 to a sample concentration of 5% with buffer;

[0081] Negative control: Tris-HCl buffer.

[0082] (2) Test operation steps

[0083] Set up sample group, negative control group and blank group, each group needs to set up 2 parallel, respectively in three groups to add different reagent solution, shake up, each group carries on HPLC analysis, determination of each tube testosterone content.

[0084] (3) Calculation formula

[0085]

[0086] 3 Test results

[0087] Table 3 5α-reductase inhibition rate

[0088] Sample Test results / % Example 1 28.89 Example 2 26.25 Example 3 26.78 Comparative Example 1 15.41 Comparative Example 2 13.38 Comparative Example 3 10.24 Negative control 2.03

[0089] Note: 5α-reductase inhibition rate retains two decimal places.

[0090] Analysis of the data in Table 3 shows that:

[0091] The data of Examples 1-3 show that the combination of bitter almond seed extract, reed extract, ophiopogon root extract and kaempferia galanga root extract used in the present application can efficiently inhibit 5α-reductase in the weight ratio range of the present application, wherein the inhibition rate of Example 1 is 28.89%;

[0092] According to the data analysis of the embodiment 1 and the comparative examples 1-3, it is found that the 5alpha-reductase inhibition rate is greatly reduced when the composition lacks the Prunus amygdalus dulcis seed extract and / or the Kaempferia galanga extract, especially the comparative example 3, which lacks both the Prunus amygdalus dulcis seed extract and the Kaempferia galanga extract, and the 5alpha-reductase inhibition rate is only 10.24%, which is much lower than that of the embodiment 1. The above data show that the Prunus amygdalus dulcis seed extract, the Phragmites communis extract, the Ophiopogon japonicus extract and the Kaempferia galanga extract are compounded in the oil control composition of the present application, which produces a synergistic effect of improving the 5alpha-reductase inhibition rate, and a better oil control effect is achieved.

[0093] Application example

[0094] The present application discloses a cosmetic (oil control essence), which contains the oil control composition prepared in the embodiment 1, and the formula is shown in Table 4.

[0095] Table 4 Formula of oil control essence

[0096]

[0097]

[0098] A preparation method of a cosmetic (oil control essence) is disclosed.

[0099] (1) Preparation of a pre-prepared phase:

[0100] ① The A3 phase is pre-stirred and uniformly dispersed.

[0101] ② The B phase is pre-stirred and completely dissolved.

[0102] ③ The C phase is pre-stirred and completely dissolved by heating to 55-60℃, and is cooled for standby use.

[0103] (2) A clean beaker is taken, the A1 phase at room temperature is measured into the beaker, and is stirred and completely dissolved. The A2 phase is slowly and repeatedly added into the liquid surface of the beaker in small amounts, and is completely wetted until no white powder particles are observed.

[0104] (3) The well-dispersed A3 phase is added, and is stirred and heated to 85-88℃. The temperature is maintained for 20 minutes, and is appropriately homogenized for 1-2 minutes.

[0105] (4) After the material is completely dissolved, the temperature is cooled to 55-60℃, the B and C phases are added, and are stirred and uniformly mixed. The temperature is continuously cooled to 45-48℃, the D phase is added, and is stirred and uniformly mixed, so that the oil control essence is obtained.

[0106] It will be apparent to those skilled in the art that the application is not limited to the details of the above-exemplified embodiments and that the present application can be implemented in other particular forms without departing from the spirit or essential characteristics of the present application. The embodiments should therefore be considered in all respects as illustrative and not restrictive, the scope of the application being indicated by the appended claims rather than by the above description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.

Claims

1. An oil control composition characterized in that, The oil-controlling composition is obtained by water extraction from a plant combination of bitter almond seeds, reed, ophiopogon root and kaempferia root, and the weight ratio of bitter almond seeds, reed, ophiopogon root and kaempferia root in the plant combination is 3-7:4-8:3-7:1-6.

2. The oil control composition of claim 1, wherein The extraction method of the oil-controlling composition is as follows: Step 1: the plant combination of bitter almond seeds, reed, ophiopogon root and kaempferia root is crushed through a 40-mesh sieve, then added into water at 65±5℃ and stirred for 2-4h, and the precipitate is removed by centrifugation, and the centrifugal liquid is concentrated by reduced pressure distillation at 60±5℃ and -0.08±0.01MPa to 25-35wt% of the mass of the centrifugal liquid, to obtain a first concentrated liquid, wherein the weight ratio of the plant combination to water is 1-3:8-15; Step 2: equal volume of 95% ethanol is added into the first concentrated liquid, and alcohol precipitation is carried out for 12-24h, and the precipitate is removed by centrifugation, and the centrifugal liquid is concentrated by reduced pressure distillation at 60±5℃ and -0.08±0.01MPa to no alcohol, to obtain a second concentrated liquid; butanediol and water are added into the second concentrated liquid, and stirred uniformly, and ultrafiltration is carried out at 0.25±0.1MPa, to obtain an ultrafiltrate, wherein the weight ratio of the second concentrated liquid, butanediol and water is 1-3:3-5:5-7; Step 3: preservative is added into the ultrafiltrate, and sterilization is carried out at 85±3℃ for 0.5-1h, to obtain the oil-controlling composition.

3. Use of the oil-controlling composition according to any one of claims 1-2 for preparing a cosmetic product.

4. Use according to claim 3, characterized in that, The cosmetic product has oil-controlling effect.

5. A cosmetic product, characterized by, The cosmetic product contains the oil-controlling composition according to any one of claims 1-2.

6. The cosmetic product according to claim 5, characterized in that, The cosmetic product contains 0.01-30wt% of the oil-controlling composition.

7. The cosmetic product according to claim 5, characterized in that, The dosage form of the cosmetic product is emulsion, cream, essence, toner, shampoo, cleansing mousse, facial cleanser or hair conditioner. The dosage form of the cosmetic product is emulsion, cream, essence, toner, shampoo, cleansing mousse, facial cleanser or hair conditioner.

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