Plasma separation equipment for detecting and evaluating acute pancreatitis by presepsin

Abstract

The invention belongs to the technical field of medical apparatuses and instruments, and discloses plasma separation equipment for presepsin detection and evaluation of acute pancreatitis, the plasma separation equipment comprises a shell, the top of the shell is provided with a protective cover, the protective cover is made of a high-strength and corrosion-resistant material, and external dust, impurities and accidentally splashed liquid can be effectively prevented from entering the shell; the separation bin is formed in the top of the shell and is used for placing a sample test tube; the rectangular mounting groove is formed in the shell and located below the separation bin, a motor is started to drive a rotating rod to rotate, the rotating rod drives a first circular plate to rotate, and an arc-shaped groove in the outer wall of the first circular plate is slidably connected with a moving strip, so that the moving strip drives a placement block to move along a sliding groove, and then a test tube slowly gets away from the rotating rod and becomes inclined; the inclined design changes the motion trail of the sample during centrifugation, so that the sample can interact with a centrifugal field more sufficiently, and different components in the sample can be separated more quickly and more thoroughly.

Description

Technical Field

[0001] The present invention belongs to the technical field of medical devices, and specifically relates to a plasma separation device for presepsin detection and evaluation of acute pancreatitis. Background Art

[0002] In the diagnosis and treatment evaluation of acute pancreatitis, Presepsin (soluble myeloid cell triggering receptor-1, sCD14-ST), as a glycoprotein released by monocytes and macrophages under inflammatory stimulation, its plasma level is positively correlated with the disease severity and is an important diagnostic marker. However, the accuracy of Presepsin detection highly depends on the purity of plasma samples. If red blood cells, white blood cells or platelet fragments remain in the plasma, these cell fragments may non-specifically bind to the antibodies in the detection reagent or interfere with the microenvironment of the immune reaction, thereby leading to false positive or false negative results and affecting clinical judgment. Therefore, to ensure the reliability of the detection results, it is necessary to strictly separate the plasma from the samples before detection to remove potential interfering factors.

[0003] There is currently a centrifugal separation device for animal plasma protein extraction with the publication number of CN220238897U, which includes an extraction box. An installation plate is fixedly connected inside the extraction box, and a centrifugal mechanism is rotatably connected inside the installation plate. The centrifugal mechanism includes a drive shaft rotatably connected inside the installation plate. A storage plate is fixedly connected to the circumferential surface of the drive shaft. A storage groove is opened on the upper end surface of the storage plate, and a separation test tube is placed in the storage groove. A fixing mechanism is arranged inside the drive shaft. The fixing mechanism includes a telescopic groove opened in the storage groove, a support plate slidably connected inside the telescopic groove, a spring fixedly connected between the lower end of the support plate and the telescopic groove, a chute opened inside the drive shaft, a connecting rod slidably connected inside the chute, a pressing plate rotatably connected to the circumferential surface of the connecting rod, and the separation test tube is located between the support plate and the pressing plate. The utility model can achieve the effect of separating animal plasma protein with simple operation.

[0004] When the above device performs centrifugal separation operations, the separation test tube is vertically placed inside the device. During centrifugation, the components with different densities in the sample are mainly stratified under the action of centrifugal force in the vertical direction. However, due to the mostly cylindrical shape of the test tube, its radial space is not fully utilized, and the vertical placement limits the movement of the sample in the radial direction, resulting in heavier components such as cell fragments and red blood cells being difficult to settle to the bottom of the test tube more quickly and thoroughly, and the stratification interface between the plasma and the cell components is not clear enough, greatly reducing the separation efficiency. Therefore, it needs to be improved and optimized. Summary of the Invention

[0005] To solve the problems raised in the above background art, the present invention provides a plasma separation device for presepsin detection and evaluation of acute pancreatitis.

[0006] To achieve the above object, the present invention provides the following technical solutions: A plasma separation device for presepsin detection and evaluation of acute pancreatitis, comprising: A housing, at the top of which is provided a protective cover made of a high-strength and corrosion-resistant material, which can effectively block dust, impurities and accidentally splashed liquids from entering the interior of the housing; A separation chamber, opened at the top of the housing, for placing sample test tubes; A rectangular installation groove, opened inside the housing and located below the separation chamber; A rotating base, arranged on the inner wall of the rectangular installation groove, for performing centrifugal separation operations on the sample test tubes; A placement mechanism is arranged inside the separation chamber for placing test tubes. By adjusting the placement mechanism, the test tubes can be tilted, increasing the movement trajectory of the samples inside the test tubes, so that the components inside can be separated from the plasma faster.

[0007] Preferably, the placement mechanism includes a driving component and a moving component. The driving component includes a motor fixedly installed on the top of the rotating base. A rotating rod is fixedly installed on the output shaft of the motor. The top of the rotating rod extends into the separation chamber and is rotatably connected to the housing.

[0008] Preferably, the moving component includes an inclination component and a lifting clamping component. The inclination component includes a circular plate two rotatably sleeved on the outer wall of the rotating rod. A plurality of sliding grooves are opened on the top of the circular plate two. A moving strip is respectively slidably installed on the inner wall of each sliding groove. A convex block is respectively arranged on the top of each moving strip. A placement block is respectively fixedly installed on the convex columns on the tops of a plurality of the moving strips.

[0009] Preferably, a rectangular groove is respectively opened on one side of a plurality of the placement blocks facing the rotating rod, and the bottom of each placement block is designed as a spherical groove, which can fit with the bottom of the test tube, increasing the contact area and making the support of the placement block for the test tube more stable.

[0010] Preferably, a circular plate one is fixedly sleeved on the outer wall of the rotating rod. The circular plate one is located above the circular plate two. A plurality of arc grooves are opened on the top of the circular plate one. A plurality of the arc grooves all penetrate through the circular plate one. The convex columns on the tops of a plurality of the moving strips are respectively slidably connected with the corresponding arc grooves. By rotating the circular plate one, the moving strips move in the sliding grooves, for changing the placement state of the test tubes.

[0011] Preferably, the lifting and clamping assembly includes a lifting group and a clamping group. The lifting group includes an installation ring fixedly sleeved on the outer wall of the rotating rod. A lead screw is rotatably installed at the top of the installation ring. A moving ring is threadedly sleeved on the outer wall of the lead screw. A limiting slide bar is fixedly installed at the top of the installation ring. The limiting slide bar penetrates through the moving ring and is slidably connected to the moving ring.

[0012] Preferably, a plurality of grooves are formed on the outer wall of the top of the rotating rod. The moving ring is adapted to and slidably connected with the grooves.

[0013] Preferably, the number of the clamping groups is the same as the number of the sliding grooves and the arc grooves, and they are all designed to be equidistantly distributed around the axis of the rotating rod.

[0014] Preferably, the clamping group includes a U-shaped frame fixedly installed on the outer wall of the moving ring. Convex grooves are respectively formed on the inner walls of both sides of the moving ring.

[0015] Preferably, two clamping blocks are respectively slidably installed on the inner walls of the two convex grooves. The mutually approaching sides of the two clamping blocks are arc-shaped. The mutually approaching sides of the two clamping blocks and the moving ring and the U-shaped frame are respectively elastically connected through a spring-damper rod assembly.

[0016] Compared with the prior art, the beneficial effects of the present invention are as follows: In the present invention, by starting the motor to drive the rotating rod to rotate, the rotating rod drives the first circular plate to rotate. The arc groove on the outer wall of the first circular plate is slidably connected with the moving strip, so that the moving strip drives the placing block to move along the sliding groove, and then the test tube slowly moves away from the rotating rod and becomes inclined. This inclined design changes the movement trajectory of the sample during centrifugation, enables the sample to interact with the centrifugal field more fully, and different components in the sample can be separated faster and more thoroughly, greatly improving the efficiency and quality of centrifugal separation.

[0017] In the present invention, by rotating the lead screw, using the threaded connection between the lead screw and the moving ring, the moving ring is driven to move vertically along the axis of the rotating rod to adjust the distance between the moving ring and the placing block. When inserting the test tube, the test tube presses the clamping block, causing the spring-damper rod assembly to contract under force. After the bottom of the test tube contacts the placing block, the spring-damper rod assembly rebounds to clamp the test tube. This design can adapt to test tubes of different sizes, and can stably clamp both the height and the diameter, providing great convenience for experimental operations. Description of the Drawings

[0018] Figure 1 is a schematic diagram of the overall structure of the present invention; Figure 2 is a schematic diagram of the internal structure of the separation chamber of the present invention; Figure 3 is a schematic diagram of the front sectional structure of the outer shell of the present invention; Figure 4Schematic diagram of the overall structure of the placement mechanism of the present invention; Figure 5 Schematic diagram of the exploded structure of the driving component of the present invention; Figure 6 Schematic diagram of the exploded structure of the lifting and clamping component of the present invention; Figure 7 Schematic diagram of the exploded structure of the clamping group of the present invention; Figure 8 Schematic diagram of the side sectional structure of the moving bar and the placement block of the present invention.

[0019] In the figure: 1. Outer shell; 101. Separation chamber; 102. Rectangular installation groove; 103. Rotation base; 2. Motor; 3. Rotating rod; 301. Groove; 4. First circular plate; 401. Arc groove; 5. Second circular plate; 501. Sliding groove; 502. Moving bar; 503. Placement block; 6. Installation ring; 601. Lead screw; 602. Limit slide bar; 7. Moving ring; 701. C-shaped frame; 7011. Convex groove; 702. Clamping block; 703. Spring damping rod assembly. Detailed implementation manner

[0020] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0021] As Figures 1 to 8 shown, the present invention provides a plasma separation device for presepsin detection and evaluation of acute pancreatitis, including: Outer shell 1, the top of which is provided with a protective cover, and the protective cover is made of a high-strength and corrosion-resistant material, which can effectively block dust, impurities and accidentally splashed liquids from entering the interior of the outer shell 1; Separation chamber 101, opened at the top of the outer shell 1, for placing sample test tubes; Rectangular installation groove 102, opened inside the outer shell 1 and located below the separation chamber 101; Rotation base 103, arranged on the inner wall of the rectangular installation groove 102, for performing centrifugal separation operations on the sample test tubes; Inside the separation chamber 101, a placement mechanism is provided for placing test tubes. By adjusting the placement mechanism, the test tubes can be tilted, increasing the movement trajectory of the samples inside the test tubes, enabling the components inside to be separated from the plasma faster. The placement mechanism includes a driving component and a moving component. The driving component includes a motor 2 fixedly installed on the top of the rotating base 103. A rotating rod 3 is fixedly installed on the output shaft of the motor 2. The top of the rotating rod 3 extends into the separation chamber 101 and is rotationally connected to the outer shell 1. The moving component includes an inclination component and a lifting and clamping component. The inclination component includes a circular plate two 5 rotatably sleeved on the outer wall of the rotating rod 3. A plurality of sliding grooves 501 are formed on the top of the circular plate two 5. A moving bar 502 is slidably installed on the inner wall of each sliding groove 501. A convex block is respectively arranged on the top of each moving bar 502. A placement block 503 is fixedly installed on the convex columns at the tops of the plurality of moving bars 502. A rectangular groove is respectively formed on one side of the plurality of placement blocks 503 facing the rotating rod 3. And the bottom of each placement block 503 is designed as a spherical groove, which can fit with the bottom of the test tube, increasing the contact area and making the support of the placement block 503 for the test tube more stable.

[0022] Adopting the above solution: The motor 2 on the top of the rotating base 103 drives the rotating rod 3 to rotate through the output shaft. The rotating rod 3 penetrates the separation chamber 101 and is rotationally connected to the outer shell 1, forming a stable vertical rotation axis. The circular plate two 5 on the outer wall of the rotating rod 3 rotates synchronously with the rotating rod 3. The moving bar 502 in the sliding groove 501 on its top is rigidly connected to the placement block 503 through a convex column, forming the basic structure for adjusting the inclination angle. Through the sliding cooperation between the arc groove 401 of the circular plate one 4 and the convex column on the top of the moving bar 502, when the circular plate one 4 rotates, the moving bar 502 moves axially along the sliding groove 501, driving the placement block 503 away from the axis of the rotating rod 3, realizing the linear adjustment of the inclination angle of the test tube. The spherical groove of the placement block 503 fits with the bottom of the test tube, and with the limiting effect of the rectangular groove, it ensures that the test tube always remains perpendicular to the contact surface of the placement block 503 during the tilting process, avoiding sample overflow or test tube slipping caused by tilting. The moving ring 7 of the lifting and clamping component is driven by the lead screw 601 and moves axially along the rotating rod 3. The U-shaped frame 701 on its outer wall elastically clamps the test tube through the spring-damper rod assembly 703, ensuring the stability of the test tube in the vertical state. When the test tube is tilted, the rotating base 103 starts, driving the tilted test tube to rotate at a high speed through the rotating rod 3, using centrifugal force to accelerate the separation of the plasma and the sample components. The contact area between the spherical groove of the placement block 503 and the bottom of the test tube is maximized, effectively dispersing the centrifugal force and avoiding the risk of rupture caused by local stress concentration at the bottom of the test tube.

[0023] As Figures 4 to 8As shown in the figure, a first circular plate 4 is fixedly sleeved on the outer wall of the rotating rod 3. The first circular plate 4 is located above the second circular plate 5. A plurality of arc-shaped grooves 401 are formed in the top of the first circular plate 4. The plurality of arc-shaped grooves 401 penetrate through the first circular plate 4. The convex columns at the tops of the plurality of moving strips 502 are respectively slidably connected with the corresponding arc-shaped grooves 401. By rotating the first circular plate 4, the moving strips 502 are moved in the sliding grooves 501 to change the placement state of the test tube. The lifting and clamping assembly includes a lifting group and a clamping group. The lifting group includes a mounting ring 6 fixedly sleeved on the outer wall of the rotating rod 3. A lead screw 601 is rotatably mounted on the top of the mounting ring 6. A moving ring 7 is threadedly sleeved on the outer wall of the lead screw 601. A limiting slide rod 602 is fixedly mounted on the top of the mounting ring 6. The limiting slide rod 602 penetrates through the moving ring 7 and is slidably connected with the moving ring 7. A plurality of grooves 301 are formed in the outer wall of the top of the rotating rod 3. The moving ring 7 is adapted to and slidably connected with the grooves 301. The number of the clamping groups is the same as that of the sliding grooves 501 and the arc-shaped grooves 401, and they are all designed to be equidistantly distributed around the axis of the rotating rod 3. The clamping group includes a U-shaped frame 701 fixedly mounted on the outer wall of the moving ring 7. Convex-shaped grooves 7011 are respectively formed in the inner walls on both sides of the moving ring 7. Two clamping blocks 702 are respectively slidably mounted on the inner walls of the two convex-shaped grooves 7011. The sides of the two clamping blocks 702 close to each other are arc-shaped. The two clamping blocks 702 and the sides of the moving ring 7 and the U-shaped frame 701 close to each other are elastically connected by a spring-damper rod assembly 703 respectively.

[0024] Adopting the above scheme: A vertical laminated structure is formed by the first circular plate 4 fixedly sleeved on the outer wall of the rotating rod 3 and the second circular plate 5. The arc-shaped grooves 401 formed in the top of the first circular plate 4 are designed to penetrate through, so that the convex columns at the tops of the moving strips 502 can be embedded into the grooves to form a sliding connection. When the rotating rod 3 rotates, the arc-shaped grooves 401 of the first circular plate 4 drive the moving strips 502 to axially move along the sliding grooves 501 through the convex columns, realizing the adjustment of the inclination angle of the test tube. The lead screw 601 on the top of the mounting ring 6 and the moving ring 7 form a screw drive. By rotating the lead screw 601, the moving ring 7 is driven to vertically move along the axis of the rotating rod 3. The double guiding design of the limiting slide rod 602 and the grooves 301 ensures that the moving ring 7 maintains concentricity with the rotating rod 3 during the lifting process, avoiding the positioning error of the test tube caused by the offset of the clamping group. Through the sliding connection between the convex-shaped grooves 7011 on the inner wall of the U-shaped frame 701 and the clamping blocks 702, and in combination with the elastic buffering characteristics of the spring-damper rod assembly 703, the clamping blocks 702 automatically contract when the test tube is inserted and rebound to clamp after contacting the placement block 503, forming a stable vertical clamping state. The arc-shaped clamping surface fits with the outer wall of the test tube, improving the clamping stability. The working principle and usage process of the present invention: First, open the protective cover on the top of the outer shell 1. In the initial state, several placement blocks 503 are located below the moving ring 7. The staff needs to adjust the distance between the moving ring 7 and the placement blocks 503 according to the height of the sample test tube to prevent the test tube from slipping. By rotating the lead screw 601, which is threadedly connected to the moving ring 7, the moving ring 7 is driven to move vertically along the axis of the rotating rod 3. When it moves to the appropriate position, insert the test tube downward between the two clamping blocks 702. During this process, the test tube will squeeze the two clamping blocks 702, causing the two spring-damper rod assemblies 703 to contract. When the bottom of the test tube contacts the placement block 503, the test tube is clamped by the spring-back of the spring-damper rod assemblies 703 on both sides of the clamping block 702, and at this time, the test tube is in a vertical state. Then, start the motor 2. The rotation of the output shaft of the motor 2 drives the rotating rod 3 to rotate, and the rotating rod 3 drives the first circular plate 4 to rotate. Due to the arc design of the arc groove 401 on the outer wall of the first circular plate 4, when the first circular plate 4 rotates, the moving strip 502 that is slidably connected to the arc groove 401 will slide along the axis of the sliding groove 501. The moving strip 502 drives the placement block 503 to move together, and the placement block 503 slowly moves away from the rotating rod 3, so that the test tube becomes inclined. Then, start the rotating base 103 inside the outer shell 1 to perform centrifugal separation operations. Finally, start the motor 2 again to make its output shaft rotate in the reverse direction, thereby driving the test tube back to the initial vertical state, which is convenient for the staff to take out the test tube.

[0025] It should be noted that in this article, relational terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the term "comprising", "including" or any other variation thereof is intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements not only includes those elements, but also includes other elements not expressly listed, or elements inherent to such process, method, article or device.

[0026] Although the embodiments of the present invention have been shown and described, for those of ordinary skill in the art, it can be understood that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principle and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A plasma separation device for presepsin detection and evaluation of acute pancreatitis, characterized in that, Comprising: A housing (1) with a protective cover provided at its top. The protective cover is made of a high-strength and corrosion-resistant material, capable of effectively blocking external dust, impurities, and accidentally splashed liquids from entering the interior of the housing (1). A separation chamber (101) opened at the top of the housing (1) for placing sample test tubes. A rectangular mounting groove (102) opened inside the housing (1) and located below the separation chamber (101). A rotating base (103) provided on the inner wall of the rectangular mounting groove (102) for performing centrifugal separation operations on sample test tubes. A placement mechanism is provided inside the separation chamber (101) for placing test tubes. By adjusting the placement mechanism, the test tubes can be tilted to increase the movement trajectory of the samples inside the test tubes, enabling the components inside to be separated from the plasma faster.

2. The plasma separation device for presepsin detection and evaluation of acute pancreatitis according to claim 1, characterized in that: The placement mechanism includes a driving component and a moving component. The driving component includes a motor (2) fixedly installed on the top of the rotating base (103). A rotating rod (3) is fixedly installed on the output shaft of the motor (2). The top of the rotating rod (3) extends into the separation chamber (101) and is rotatably connected to the housing (1).

3. The plasma separation device for presepsin detection and evaluation of acute pancreatitis according to claim 2, characterized in that: The moving component includes an inclination component and a lifting and clamping component. The inclination component includes a second circular plate (5) rotatably sleeved on the outer wall of the rotating rod (3). A plurality of sliding grooves (501) are opened on the top of the second circular plate (5). A moving bar (502) is slidably installed on the inner wall of each sliding groove (501). A convex block is provided at the top of each moving bar (502). A placement block (503) is fixedly installed on the convex columns at the tops of the plurality of moving bars (502).

4. The plasma separation device for presepsin detection and evaluation of acute pancreatitis according to claim 3, characterized in that: A rectangular groove is opened on one side of each of the plurality of placement blocks (503) facing the rotating rod (3). The bottom of each placement block (503) is designed as a spherical groove, which can fit with the bottom of the test tube to increase the contact area and make the support of the placement block (503) for the test tube more stable.

5. The plasma separation device for presepsin detection and evaluation of acute pancreatitis according to claim 3, characterized in that: A first circular plate (4) is fixedly sleeved on the outer wall of the rotating rod (3). The first circular plate (4) is located above the second circular plate (5). A plurality of arc-shaped grooves (401) are opened on the top of the first circular plate (4). The plurality of arc-shaped grooves (401) penetrate through the first circular plate (4). The convex columns at the tops of the plurality of moving bars (502) are respectively slidably connected to the corresponding arc-shaped grooves (401). By rotating the first circular plate (4), the moving bars (502) move in the sliding grooves (501) to change the placement state of the test tubes.

6. The plasma separation device for presepsin detection and evaluation of acute pancreatitis according to claim 3, characterized in that: The lifting and clamping component includes a lifting group and a clamping group. The lifting group includes a mounting ring (6) fixedly sleeved on the outer wall of the rotating rod (3). A lead screw (601) is rotatably installed on the top of the mounting ring (6). A moving ring (7) is threadedly sleeved on the outer wall of the lead screw (601). A limiting slide bar (602) is fixedly installed on the top of the mounting ring (6). The limiting slide bar (602) penetrates through the moving ring (7) and is slidably connected to the moving ring (7).

7. The plasma separation device for presepsin detection and evaluation of acute pancreatitis according to claim 6, characterized in that: A plurality of grooves (301) are formed in the outer wall of the top of the rotating rod (3), and the moving ring (7) is adapted to the grooves (301) and is slidably connected thereto.

8. The plasma separation device for presepsin detection and evaluation of acute pancreatitis according to claim 6, wherein: The number of the clamping groups is the same as the number of the sliding grooves (501) and the arc grooves (401), and they are all designed to be equidistantly distributed around the axis of the rotating rod (3).

9. The plasma separation device for presepsin detection and evaluation of acute pancreatitis according to claim 8, characterized in that: The clamping group includes a U-shaped frame (701) fixedly installed on the outer wall of the moving ring (7), and convex grooves (7011) are respectively formed on the inner walls of both sides of the moving ring (7).

10. The plasma separation device for presepsin detection and evaluation of acute pancreatitis according to claim 9, characterized in that: Two clamping blocks (702) are respectively slidably installed on the inner walls of the two convex grooves (7011). The sides of the two clamping blocks (702) close to each other are arc-shaped. The two clamping blocks (702) and the sides of the moving ring (7) and the U-shaped frame (701) close to each other are elastically connected by spring-damper rod assemblies (703).

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