A microplate for drug sensitivity testing

Abstract

The application discloses a kind of micro-well plate of drug sensitivity detection, it is related to microorganism detection technical field, including bottom plate and cover plate, the cultivation plate body is integrally fixed in bottom plate top, and the height difference of cultivation plate body outer edge and bottom plate forms step.This application only needs to operate the action of palm pressing in the use process of the application, that is, the synchronous liquid supplementing operation to each hole position on the cultivation plate body can be realized simultaneously, the liquid supplementing efficiency is significantly improved, and because the action flow of continuous execution flip liquid exchange, vertical liquid supplementing and back normal pressing is extremely simplified, not only can effectively reduce the learning cost of user, be conducive to large-scale production and popularization, compared with traditional liquid exchange of pipette, the cell liquid exchange time of the application is greatly shortened, effectively avoid the cell dehydration and death for long time caused by long liquid exchange time, the quality of cultivated cell is improved, and then the accuracy and success rate of experiment are improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of microorganism detection, in particular to a microplate for drug sensitivity detection. BACKGROUND

[0002] Microorganisms are small in size, and the diameters of common coccus are about 0.5-1 μm, and the lengths of medium-sized bacillus are about 2-5 μm and the widths are about 0.3-1 μm, which cannot be seen by naked eyes. When the microorganisms divide and proliferate in solid agar medium, many cells are accumulated together to form small bacterial plaques or clusters. Under the action of antibiotics, proliferative bodies with different diameters are formed. The drug sensitivity test period can be significantly shortened by observing the proliferative bodies through a microscopic imaging device, compared with the traditional turbidity method. In recent years, multi-well microplates are often used as carriers for micro-reaction, and the microplates have good sealing performance and high biological safety. The morphology and size of microcolonies in the micro-culture wells can be continuously observed through a microscope, and the drug sensitivity can be quickly and accurately determined.

[0003] However, a large number of cell culture plates are needed for parallel tests when such research is carried out, and the cell culture process needs to be replaced with liquid. Laboratory operations usually use a pipette gun to replace the liquid. The pipette gun is used to suck the waste culture liquid in one well of the cell culture plate each time. In order to prevent cross contamination, the gun head is replaced. In this way, since the number of cell culture plates is large, and each cell culture plate contains a plurality of cell culture wells, there are many problems in replacing the liquid in each cell culture well with the pipette gun. First, the experimental task is heavy, time-consuming and labor-intensive. Second, the consumption of a large number of pipette gun heads causes resource waste and increases the burden of medical waste treatment. Most importantly, the effect of cell culture cannot be guaranteed, and even the experiment can fail directly. Specifically, first, when the liquid is replaced with the pipette gun, the waste liquid is easily not completely sucked, resulting in residual waste liquid. Second, the pipette gun is easily misused to contact the tip of the gun head with the bottom of the well, thereby damaging the cell layer. Finally, due to the long operation time, the cell culture well cannot be added with new culture liquid in time after the waste liquid is sucked, resulting in the death of the cells due to long-term dehydration. SUMMARY

[0004] The present application aims to provide a microplate for drug sensitivity detection to solve the problems in the background art.

[0005] To achieve the above object, the present application provides the following technical scheme.

[0006] The utility model provides a kind of drug sensitivity detection's microplate, including bottom plate and cover plate, the bottom plate top integral fixed has incubation plate body, and the height difference of incubation plate body outer edge and bottom plate forms step, the cover plate is buckled on the top of incubation plate body, and the opening between cover plate bottom and step inner wall is tightly matched, the inner wall of cover plate opening is equipped with the recess corresponding with the hole site of incubation plate body, and recess inner top is embedded with water-absorbing column, and the material of water-absorbing column is non-toxic and strong water-absorbing polyurethane sponge material, recess inner bottom is embedded with stainless steel ring, and stainless steel ring is fixed with semi-permeable membrane inside for isolating cell macromolecule and allowing only small molecule medium to pass.

[0007] Further, the top of the cover plate is provided with a water storage cavity, and the water storage cavity is sealed from the recess.

[0008] Further, the side end of the water storage cavity is communicated with a liquid supplement pipe, and a plug is arranged at the opening of the liquid supplement pipe.

[0009] Further, a pressing plate is arranged inside the water storage cavity, and the pressing plate is made of transparent polycarbonate.

[0010] Further, a scale mark is arranged on the surface of the pressing plate away from the liquid supplement pipe, and a connecting column is fixedly connected to the bottom of the pressing plate corresponding to the position of the recess.

[0011] Further, a control assembly is communicated between the water storage cavity and the recess, the control assembly includes a valve body and a side hole, the valve body is arranged through the axial through hole of the middle part of the water-absorbing column and the semi-permeable membrane, and a side hole is arranged on the end of the valve body facing the hole site of the incubation plate body.

[0012] Further, the control assembly further includes a throat pipe and a distribution cavity, the inner wall of the valve body is fixed with a throat pipe, and the side of the throat pipe connected with the water storage cavity is provided with a distribution cavity.

[0013] Further, the control assembly further includes a valve core, the valve core with a circular truncated cone structure is arranged on the other side of the throat pipe, and the end plane of the valve core abuts against the connecting column.

[0014] Further, the control assembly further includes a sealing plate, the bottom of the valve core is fixedly connected with a sealing plate, and the thickness of the sealing plate is greater than the opening width of the side hole.

[0015] Further, the control assembly further includes a spring, the bottom of the sealing plate is connected with a spring, and the sealing plate is elastically connected with the bottom end of the valve body through the spring. Beneficial effects

[0016] During use of the present application, when liquid exchange is performed, the cell culture plate and the cover plate are turned over and inverted as a whole, the original culture medium in the hole position of the cell culture plate body is absorbed by the water absorption column made of polyurethane sponge material under the action of gravity, and the culture medium is absorbed by the water absorption column. Through the innovative design of the flip liquid exchange method, the liquid exchange efficiency of the cell culture plate body can be improved, especially when batch liquid exchange operation is performed on the multi-hole cell culture plate, the liquid exchange efficiency can be significantly improved because the liquid exchange operation does not need to be performed one by one using a pipette, and the problem of incomplete absorption of waste liquid caused by the use of a pipette for liquid exchange can be avoided. The operation is simple, and resource waste caused by consumption of a large number of pipette tips is avoided.

[0017] During use of the present application, when subsequent liquid supplementing or sample adding operations are performed, the cell culture plate and the cover plate are first erected as a whole, then the prepared preparation mixed with an antibacterial drug is added to the water storage cavity through the liquid supplementing pipe on the side of the cover plate, the addition amount of the preparation is accurately controlled through the scale mark arranged on the surface of the pressing plate during this process, and finally the cell culture plate and the cover plate are placed in the original position as a whole, the cover plate is slightly shaken to uniformly distribute the preparation in the distribution cavity at the top end of the valve body. Through the operation of erecting the cell culture plate and the cover plate as a whole and supplementing liquid through the side liquid supplementing pipe at one time, the uniform distribution of the preparation in each distribution cavity is ensured while the liquid supplementing amount is accurately controlled, the problem of uneven distribution caused by repeated operation of a pipette one by one is solved, and the operation intensity is reduced, thereby ensuring the consistency of the test standard.

[0018] 3、During use of the present application, the operator only needs to perform the action of palm pressing, and synchronous liquid supplementing operation on each hole position of the cell culture plate body can be simultaneously realized, the liquid supplementing efficiency is significantly improved, and the action flow of continuous flip liquid exchange, vertical liquid supplementing and back-to-normal pressing is greatly simplified. Therefore, the learning cost of the user can be effectively reduced, large-scale production and popularization are facilitated, compared with the traditional liquid exchange using a pipette, the cell liquid exchange time is greatly shortened, the problem of cell dehydration and death caused by long liquid exchange time can be effectively avoided, the quality of the cultured cells is improved, and the accuracy and success rate of the experiment are improved. BRIEF DESCRIPTION OF DRAWINGS

[0019] Figure 1 It is a schematic diagram of the overall structure of the device of the present application;

[0020] Figure 2 It is a schematic diagram of the cross-sectional structure of the device of the present application;

[0021] Figure 3 It is a schematic diagram of the split structure of the device of the present application;

[0022] Figure 4 It is a schematic diagram of the vertical liquid supplementing state of the present application;

[0023] Figure 5It is a cover plate cross section structure schematic diagram of the application;

[0024] Figure 6 It is a control assembly structure schematic diagram of the application.

[0025] In the figure: 1, bottom plate; 2, culture plate body; 3, step; 4, cover plate; 5, recess; 6, water absorption column; 7, stainless steel ring; 8, semi-permeable membrane; 9, water storage cavity; 10, liquid supplement pipe; 11, plug; 12, pressing plate; 13, scale mark; 14, connecting column; 15, control assembly; 1501, valve body; 1502, side hole; 1503, throat pipe; 1504, distribution cavity; 1505, valve core; 1506, sealing plate; 1507, spring. DETAILED DESCRIPTION

[0026] The embodiments of the present application will be further described below in conjunction with the drawings and examples. The following examples are used to illustrate the present application, but cannot be used to limit the scope of the present application.

[0027] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs; the terminology used in the specification herein is for describing particular embodiments only and is not intended to be limiting of the application; the use of the terms "including," "comprising," "having" and variations thereof herein is meant to encompass the items listed thereafter and equivalents thereof as well as additional items.

[0028] In the description of the embodiments of the present application, unless otherwise explicitly specified and limited, the technical terms "mounting", "connecting", "connecting", "fixing" and the like should be understood in a broad sense, for example, it can be fixedly connected, or it can be detachably connected, or it can be integrated; it can be mechanically connected, or it can be electrically connected; it can be directly connected, or it can be indirectly connected through an intermediate medium; it can be the internal communication of two elements or the interaction relationship between two elements. For those skilled in the art, the specific meaning of the above terms in the embodiments of the present application can be understood according to the specific circumstances.

[0029] Please refer to Figures 1 to 3 The micro-well plate for drug sensitivity detection provided by the application comprises a bottom plate 1 and a cover plate 4, the top of the bottom plate 1 is integrally fixed with a culture plate body 2, the height difference between the outer edge of the culture plate body 2 and the bottom plate 1 forms a step 3, the cover plate 4 is buckled above the culture plate body 2, and the opening at the bottom of the cover plate 4 is tightly fitted with the inner wall of the step 3, the inner wall of the opening of the cover plate 4 is provided with a recess 5 corresponding to the hole position of the culture plate body 2, a water absorption column 6 is embedded at the top of the recess 5, the material of the water absorption column 6 is non-toxic and strong water-absorbing polyurethane sponge material, a stainless steel ring 7 is embedded at the bottom of the recess 5, and the semi-permeable membrane 8 for isolating cell macromolecules and allowing only small molecule culture medium to pass through is fixed in the stainless steel ring 7.

[0030] Specific operation as follows, the cover plate 4 buckle on the culture plate body 2, and make the notch 5 position and the hole position on the culture plate body 2 corresponding, when liquid exchange operation, the cell culture plate and cover plate 4 overall flip inversion, the original culture medium in the culture plate body 2 hole position under the action of gravity through the semi-permeable membrane 8 and by polyurethane sponge material made of water absorption column 6 is absorbed, while the macromolecular bacteria are intercepted in the culture plate body 2 hole position, through the use of water absorption column 6 avoid in subsequent back to normal placement waste liquid reverse osmosis back flow, this can be through slight vibration, so that the residual waste liquid in the culture hole wall is completely absorbed by water absorption column 6, the application can improve the culture plate body 2 liquid exchange efficiency through the innovative design of flip liquid exchange method, especially in the batch liquid exchange operation for multi well culture plate, not only can significantly improve the liquid exchange efficiency because of no need to use pipette operation one by one, but also can avoid the problem of incomplete suction caused by using pipette liquid exchange, simple operation, avoid the waste of resources caused by a large number of pipette tip consumption.

[0031] Please refer to Figures 4 to 5 , the cover plate 4 top is equipped with water storage cavity 9, and the water storage cavity 9 and the notch 5 exist sealed partition, the water storage cavity 9 side end is communicated with the liquid supplement pipe 10, and the liquid supplement pipe 10 opening is equipped with the plug 11, the water storage cavity 9 inside is provided with the pressing plate 12, and the pressing plate 12 adopts transparent polycarbonate material, the pressing plate 12 surface deviating from the liquid supplement pipe 10 one end is equipped with the scale mark 13, and the pressing plate 12 bottom is fixedly connected with the connecting column 14 corresponding to the notch 5 position.

[0032] In the subsequent liquid supplement or sample addition operation, first, the cell culture plate and cover plate 4 are erected as a whole, and then the pre mixed preparation mixed with antibacterial drugs is added to the water storage cavity 9 through the liquid supplement pipe 10 on the side of the cover plate 4, the scale mark 13 on the surface of the pressing plate 12 is used to accurately control the amount of preparation added, and finally the cell culture plate and cover plate 4 are placed back to normal, and the cover plate 4 is slightly shaken to make the preparation in the water storage cavity 9 evenly distributed in the distribution cavity 1504 on the top of the valve body 1501, the application can ensure the equal distribution of the preparation in each distribution cavity 1504 by erecting the cell culture plate and cover plate 4 as a whole and supplementing the liquid through the side liquid supplement pipe 10 at one time, which can solve the problem of uneven distribution caused by repeated operation of pipette and high operation intensity, and ensure the consistency of test standard.

[0033] Please refer to Figure 6 , the water storage cavity 9 and the notch 5 are communicated with the control assembly 15.

[0034] The control assembly 15 comprises a valve body 1501 and a side hole 1502, the valve body 1501 is arranged through the axial through hole in the middle of the water column 6 and the semi-permeable membrane 8, and the valve body 1501 is circumferentially provided with a side hole 1502 at one end of the hole position of the culture plate body 2, the control assembly 15 further comprises a throat pipe 1503 and a distribution cavity 1504, the inner wall of the valve body 1501 is fixed with the throat pipe 1503, and the side of the throat pipe 1503 connected with the water storage cavity 9 is provided with the distribution cavity 1504, the control assembly 15 further comprises a valve core 1505, the other side of the throat pipe 1503 is provided with a circular truncated cone structure of the valve core 1505, and the end plane of the valve core 1505 is in abutment with the connecting column 14, the control assembly 15 further comprises a sealing plate 1506, the bottom of the valve core 1505 is fixedly connected with the sealing plate 1506, and the thickness of the sealing plate 1506 is greater than the opening width of the side hole 1502, the control assembly 15 further comprises a spring 1507, the bottom of the sealing plate 1506 is connected with the spring 1507, and the sealing plate 1506 is elastically connected with the bottom end of the valve body 1501 through the spring 1507.

[0035] Specific operation is as follows, the user uses the palm to press on the pressing plate 12, the connecting column 14 fixedly connected to the inner wall of the pressing plate 12 is in abutment with the end of the valve core 1505 in the valve body 1501 during the process of the pressing plate 12 overcoming the elastic force of the spring 1507 to descend, so that the throat pipe 1503 originally closed by the valve core 1505 is opened, and the preparation in the distribution cavity 1504 is evenly distributed through the open throat and flows out from the side hole 1502 circumferentially arranged at the bottom end of the valve body 1501, the present application only needs the operator to perform the action of palm pressing, which can realize the synchronous liquid supplementing operation on each hole position of the culture plate body 2, the liquid supplementing efficiency is significantly improved, and because the action process of continuously performing the liquid changing, vertical liquid supplementing and returning pressing is extremely simplified, not only can effectively reduce the learning cost of the user, but also is conducive to large-scale production and promotion, compared with the traditional liquid changing of the liquid gun, the present application can shorten the time of cell liquid changing, effectively avoid the cell dehydration and death caused by long liquid changing time, improve the quality of cultured cells, and further improve the accuracy and success rate of experiments.

[0036] In summary, when using the drug sensitivity detection microwell plate:

[0037] Firstly, the cover plate 4 is buckled on the culture plate body 2, and the notch 5 position corresponds to the hole position on the culture plate body 2. When the liquid exchange operation is carried out, the cell culture plate and the cover plate 4 are turned over and inverted as a whole. The original culture medium in the hole position of the culture plate body 2 passes through the semi-permeable membrane 8 under the action of gravity and is absorbed by the water absorption column 6 made of polyurethane sponge material. The macromolecular bacterial population is intercepted in the hole position of the culture plate body 2. The use of the water absorption column 6 avoids the reverse infiltration of the waste liquid when the subsequent reverse placement is placed. During this period, slight vibration can be used to make the residual waste liquid in the culture hole wall completely absorbed by the water absorption column 6. The application can improve the liquid exchange efficiency of the culture plate body 2 through the innovative design of the turnover liquid exchange method. Especially when the batch liquid exchange operation is carried out on the multi-well culture plate, the liquid exchange efficiency can be significantly improved because the liquid exchange gun does not need to be used for individual operation. Moreover, the problem of incomplete absorption of waste liquid caused by the use of the liquid exchange gun can be avoided. The operation is simple, and the resource waste caused by the consumption of a large number of liquid transfer gun heads is avoided;

[0038] Secondly, during the subsequent liquid supplementing or sample adding operation, the cell culture plate and the cover plate 4 are erected as a whole, and the preparation mixed with the antibacterial drug is added to the water storage cavity 9 through the liquid supplementing pipe 10 on the side of the cover plate 4. During this period, the scale mark 13 arranged on the surface of the pressing plate 12 is used to accurately control the addition amount of the preparation. Finally, the cell culture plate and the cover plate 4 are placed in the reverse direction, and the preparation in the water storage cavity 9 is evenly distributed in the distribution cavity 1504 at the top end of the valve body 1501 by slightly shaking the cover plate 4. The application can accurately control the liquid supplementing amount and ensure the equal distribution of the preparation in each distribution cavity 1504 by erecting the cell culture plate and the cover plate 4 as a whole and supplementing the liquid through the side liquid supplementing pipe 10 at one time. The problem of uneven distribution caused by repeated operation of the liquid transfer gun and the high operation intensity are solved, and the consistency of the test standard is ensured;

[0039] Finally, the user presses the palm on the pressing plate 12, and the connecting column 14 fixed on the inner wall of the pressing plate 12 abuts against the end of the valve core 1505 in the corresponding valve body 1501 during the process of the pressing plate 12 overcoming the elastic force of the spring 1507 and falling, so that the throat 1503 originally closed by the valve core 1505 is opened, and the preparation in the distribution cavity 1504 is evenly distributed and flows out from the side hole 1502 arranged at the bottom of the valve body 1501. The present application only needs the operator to perform the action of pressing the palm, and the synchronous liquid supplementing operation of each hole position on the culture plate body 2 can be realized, the supplementing efficiency is improved, and the action process of continuously performing the turning, vertical supplementing and returning is extremely simplified, which can effectively reduce the learning cost of the user, is beneficial to large-scale production and promotion, compared with the traditional liquid changing gun, the liquid changing time of the present application is greatly shortened, the long-time dehydration and death of cells caused by long-time liquid changing can be effectively avoided, the quality of the cultured cells is improved, and the accuracy and success rate of the experiment are improved.

[0040] It should be noted that in this document, the terms "comprise", "comprising", or any other variant thereof are intended to cover non-exclusive inclusions, such that processes, methods, articles, or apparatuses that comprise a list of elements do not only include those elements, but also other elements not expressly listed, or other elements inherent to such processes, methods, articles, or apparatuses.

[0041] The principles and implementation modes of the present application are described by using specific examples in this document, and the above examples are only used to help understand the method of the present application and its core idea. The above description is only the preferred implementation mode of the present application, and it should be pointed out that due to the limitation of language expression, there are infinite specific structures, and for ordinary skilled persons in the technical field, some improvements, decorations or changes can be made without departing from the principles of the present application, or the above technical features can be combined in an appropriate way; these improvements, decorations, changes or combinations, or the direct application of the inventive concept and technical scheme to other occasions without improvement, should be regarded as the protection scope of the present application.

Claims

1. A microplate for drug sensitivity testing comprising a base plate and a cover plate, characterised in that, The bottom plate top is integrally fixed with a culture plate body, and the height difference between the outer edge of the culture plate body and the bottom plate forms a step, the cover plate is buckled above the culture plate body, and the cover plate bottom opening is tightly matched with the inner wall of the step, the cover plate opening inner wall is provided with a notch corresponding to the culture plate body hole, the notch inner top is embedded with a water absorption column, the material of the water absorption column is non-toxic and strong water absorption polyurethane sponge material, the notch inner bottom is embedded with a stainless steel ring, and the stainless steel ring is fixed with a semi-permeable membrane for isolating cell macromolecules and allowing only small molecule culture medium to pass; the top of the cover plate is provided with a water storage cavity, the water storage cavity is provided with a pressing plate, and the pressing plate bottom is fixedly connected with a connecting column corresponding to the notch position; the water storage cavity and the notch are communicated with a control assembly, the control assembly includes a valve body, a side hole, a throat pipe, a distribution cavity, a valve core and a sealing plate, the valve body is axially through the hole set along the middle part of the water absorption column and the semi-permeable membrane, and the valve body is provided with a side hole on the side facing the hole of the culture plate body, the valve body inner wall is fixed with a throat pipe, and the side of the throat pipe connected with the water storage cavity is provided with a distribution cavity, the other side of the throat pipe is provided with a valve core with a circular truncated cone structure, and the valve core end plane is in abutment with the connecting column, the valve core bottom is fixedly connected with a sealing plate, and the sealing plate thickness is greater than the side hole opening width; shake the cover plate so that the preparation in the water storage cavity is uniformly distributed in the distribution cavity at the top of the valve body.

2. The microplate for drug sensitivity testing according to claim 1, wherein, There is a sealed partition between the water storage cavity and the notch.

3. The microplate for drug sensitivity testing according to claim 2, wherein, The water storage cavity side end is communicated with a liquid supplement pipe, and the liquid supplement pipe opening is provided with a plug.

4. The microplate for drug sensitivity testing according to claim 3, wherein, The pressing plate is made of transparent polycarbonate material.

5. The microplate for drug sensitivity testing according to claim 4, wherein, The pressing plate surface away from the liquid supplement pipe end is provided with a scale mark.

6. The microplate for drug sensitivity testing according to claim 1, wherein, The control assembly further includes a spring, the sealing plate bottom is connected with a spring, and the sealing plate is elastically connected with the valve body bottom end through the spring.

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