A composition for promoting cartilage repair and maintaining joint health, and its preparation process and application

Abstract

The present invention discloses a composition for promoting cartilage repair and maintaining joint health, its preparation process and application, and relates to the field of functional food technology. The composition is made of the following components by weight: 21.1-90 parts of a multi-protein complex, 0.1-3 parts of cherry powder, N The composition comprises 0.01-2 parts of acetylglucosamine, 0.01-2 parts of kudzu root powder, 0.1-1.5 parts of a calcium compound, and 0.01-0.2 parts of turmeric; the multi-protein complex includes 0.1-10 parts of skate collagen peptide, 1-30 parts of bone collagen peptide powder, and 20-50 parts of fish collagen peptide. The composition of the present invention can promote cartilage repair and achieve the purpose of daily joint health maintenance, and has good application prospects.

Description

Technical Field

[0001] The present invention relates to the technical field of nutritional compositions, and in particular to a composition for promoting cartilage repair and maintaining joint health, and a preparation process and application thereof. Background Art

[0002] Articular cartilage refers to the thin layer of hyaline cartilage that covers the joint surfaces of bone ends. Composed primarily of type II collagen, proteoglycans, chondrocytes, and water, it possesses a certain degree of elasticity and a smooth surface. It plays a vital role in buffering mechanical stress, resisting pressure, enhancing bone elasticity, and supporting joint movement. However, articular cartilage lacks a lymphatic and blood supply. Nutrients and waste products from cartilage metabolism are absorbed and excreted through diffusion from surrounding tissues. Consequently, damaged articular cartilage repairs slowly.

[0003] Articular cartilage can be morphologically divided into four layers: the tangential zone, the transitional zone, the radiating zone, and the subchondral bone plate. The radiating zone and subchondral bone plate are separated by the tide line. Therefore, articular cartilage can be divided into four zones: the superficial zone, the intermediate zone, the deep zone, and the highly mineralized, calcified cartilage zone. The tangential zone, located at the outermost layer of the cartilage, is primarily composed of collagen fibers and chondrocytes. In the transitional and radiating zones, the collagen fibers gradually shift in orientation to an oblique or perpendicular orientation to the articular surface, primarily responsible for compressive resistance. Subchondral bone provides mechanical support for the articular cartilage and plays a crucial role in maintaining joint homeostasis.

[0004] The clinical manifestations of cartilage damage include: (1) joint pain: When cartilage is damaged, patients usually experience joint pain, which may be intermittent or related to movement; (2) joint swelling: The damage causes an inflammatory response in the tissues around the joint, causing joint swelling; (3) joint instability: Cartilage damage may cause irregularities in the joint surface, making the joint feel unstable during movement; (4) limited joint movement: Due to the irregularities in the joint surface caused by cartilage damage, the normal range of movement may be limited; (5) joint friction: The irregular surface of the cartilage may cause a friction sensation during joint movement, especially when bending or straightening the joint.

[0005] Currently, common methods for treating cartilage damage include: (1) arthroscopic surgery: arthroscopic surgery is used to remove damaged cartilage tissue and perform cartilage transplantation to transplant healthy cartilage tissue to the damaged area; (2) conservative treatment, including the use of steroidal anti-inflammatory drugs to relieve pain and control inflammation; (3) minimally invasive surgery: minimally invasive surgery can repair cartilage damage without opening the joint; (4) joint replacement surgery: in cases of severe cartilage damage, joint replacement surgery is performed to improve and restore joint function. However, most treatments are unable to produce enough new tissue to repair the damaged cartilage.

[0006] Chinese patent CN117045778A discloses a composition for improving joint cartilage health and its production process. The composition comprises the following components, by weight: 5-10% collagen peptide, 10-20% rosehip seed extract, 1-5% concentrated olive juice freeze-dried powder, 0.1-0.3% vitamin C, 1-3% fruit juice powder, and the balance a calcium supplement. This invention, packaged with collagen peptide, rosehip seed extract, and concentrated olive juice freeze-dried powder as the main ingredients, supplemented with vitamin C, fruit juice powder, and a calcium supplement, is said to improve joint cartilage health, prevent and repair osteoarticular cartilage damage, increase bone strength, and regenerate and repair joint cartilage.

[0007] Currently, there is little research on promoting cartilage repair, and the existing compositions for improving articular cartilage health are not effective and cannot meet the actual needs of patients. Therefore, there is an urgent need to develop a product that can safely and efficiently promote articular cartilage damage with minimal side effects. Summary of the Invention

[0008] In order to overcome the above-mentioned deficiencies of the prior art, the purpose of the present invention is to provide a composition for promoting cartilage repair and maintaining joint health, as well as its preparation process and application.

[0009] The present invention uses multiple protein complexes (ray fish collagen peptide, bone collagen peptide powder, fish collagen peptide), cherry powder, N -Acetyl glucosamine, kudzu root powder, calcium compounds, turmeric, through scientific and reasonable proportions, especially the addition of cherry powder and kudzu root powder, can promote the synthesis of multiple protein complexes (ray collagen peptide, bone collagen peptide powder, fish collagen peptide), N -The absorption of acetyl glucosamine promotes cartilage repair.

[0010] In order to achieve the above-mentioned object of the invention, the technical solution of the present invention is as follows:

[0011] In one aspect, the present invention provides a composition for promoting cartilage repair and maintaining joint health, wherein the composition is made of the following components in parts by weight:

[0012] Multi-protein complex 21.1-90 parts, cherry powder 0.1-3 parts, N-acetylglucosamine 0.01-2 parts, kudzu root powder 0.01-2 parts, calcium compound 0.1-1.5 parts, turmeric 0.01-0.2 parts;

[0013] The multi-protein complex comprises 0.1-10 parts of ray collagen peptide, 1-30 parts of bone collagen peptide powder and 20-50 parts of fish collagen peptide.

[0014] Specifically, the composition is made of the following components in parts by weight: 0.1-6 parts of ray collagen peptide, 10-20 parts of bone collagen peptide powder, 30-40 parts of fish collagen peptide, 0.5-1.5 parts of cherry powder, 0.1-1.5 parts of N-acetylglucosamine, 0.5-1.5 parts of kudzu root powder, 0.6-1 parts of calcium compound, and 0.08-0.15 parts of turmeric.

[0015] Furthermore, the composition is made of the following components in parts by weight: 1 part of ray collagen peptide, 14 parts of bone collagen peptide powder, 36 parts of fish collagen peptide, 1 part of cherry powder, N -1 part of acetylglucosamine, 1.5 parts of kudzu root powder, 1.5 parts of calcium compound, 0.1 part of turmeric.

[0016] Specifically, the calcium compound is selected from one or more of calcium citrate, calcium carbonate, and calcium gluconate.

[0017] Furthermore, the calcium compound is a combination of calcium citrate and calcium gluconate.

[0018] Specifically, the mass ratio of calcium citrate to calcium gluconate is (1-3):1.

[0019] Furthermore, the mass ratio of the calcium citrate to the calcium gluconate is 2:1.

[0020] Specifically, the cherries are selected from one or more of acerola cherries, sweet cherries, and sour cherries.

[0021] Furthermore, the cherries are sour cherries.

[0022] Specifically, the preparation method of the cherry powder is:

[0023] (1) Add 3-5 times the amount of water to the cherry to squeeze the juice to obtain cherry juice;

[0024] (2) Add enzyme to the cherry juice to perform enzymolysis, inactivate the enzyme, concentrate, and dry to obtain the product.

[0025] Furthermore, the juice in step (1) is squeezed and then passed through a 40-mesh sieve.

[0026] Specifically, the enzyme used for the enzymatic hydrolysis in step (2) is selected from one or more of cellulase, pectinase, and hemicellulase.

[0027] Furthermore, the enzyme used in the enzymatic hydrolysis in step (2) is cellulase.

[0028] Specifically, in step (2), the material-liquid ratio of the enzyme to the cherry juice puree is (1-3):1.

[0029] Furthermore, in step (2), the material-liquid ratio of the enzyme to the cherry juice puree is 1:1, 2.5:1 or 3:1.

[0030] Furthermore, in step (2), the material-liquid ratio of the enzyme to the cherry juice puree is 2.5:1.

[0031] Specifically, the enzymatic hydrolysis time in step (2) is 40-60 min; the enzymatic hydrolysis temperature is 25-35°C;

[0032] Furthermore, the enzymatic hydrolysis time in step (2) is 50 min; and the enzymatic hydrolysis temperature is 25°C.

[0033] Specifically, the enzyme inactivation temperature is 80-90° C., and the enzyme inactivation time is 5-10 minutes.

[0034] Furthermore, the enzyme inactivation temperature is 90° C. and the enzyme inactivation time is 8 minutes.

[0035] Furthermore, the concentration in step (2) includes but is not limited to vacuum concentration, freeze concentration, and salting-out concentration.

[0036] Furthermore, the concentration in step (2) is vacuum concentration.

[0037] Furthermore, the vacuum degree of the vacuum concentration in step (2) is 0.05-0.08 MPa;

[0038] Furthermore, the temperature of the vacuum concentration in step (2) is 45-70°C;

[0039] Furthermore, the temperature of the vacuum concentration in step (2) is 60°C.

[0040] Furthermore, the concentration in step (2) is to concentrate to a relative density of 1.25-1.30.

[0041] Specifically, the drying in step (2) includes but is not limited to vacuum drying.

[0042] Furthermore, the vacuum drying conditions are 0.06-0.08Mpa and the temperature is 70-80°C.

[0043] Furthermore, the vacuum drying conditions are 0.08 MPa and 80°C.

[0044] Furthermore, the drying in step (2) is performed until the water content of the extract is less than 3%.

[0045] Furthermore, the product in step (2) is dried and then crushed through a 60-mesh sieve.

[0046] Specifically, the preparation method of the kudzu root powder is as follows: adding water to the kudzu root, boiling it, beating it into pulp, filtering it, discarding the precipitate, taking the filtrate and drying it to obtain the kudzu root powder.

[0047] Furthermore, the kudzu root is fresh kudzu root with the skin removed.

[0048] Furthermore, the cooking time is 60-100 minutes.

[0049] Furthermore, the amount of water added is 8-10 times the amount of Pueraria root.

[0050] According to some embodiments of the present invention, the preparation method of the kudzu root powder is: peeling the fresh kudzu root, adding 8-10 times the amount of water and boiling for 60-100 minutes, beating, filtering, letting the filtrate stand for 10-12 hours, discarding the precipitate, and drying the filtrate at 30-50°C to obtain the kudzu root powder.

[0051] In another aspect, the present invention provides a method for preparing the above-mentioned composition, comprising the following steps:

[0052] (1) Grind and mix the ray collagen peptide, bone collagen peptide powder and fish collagen peptide to obtain a multi-protein complex;

[0053] (2) Mix cherry powder, kudzu root powder and turmeric to obtain mixture 1;

[0054] (3) Add the multi-protein complex to mixture 2, and then add N -acetylglucosamine and a calcium compound to obtain a composition.

[0055] In another aspect, the present invention provides use of the above composition in preparing a product for promoting cartilage repair.

[0056] In the present invention, the ray fish collagen peptide has the function of stimulating osteoblast activity, promoting bone cell proliferation, improving calcium absorption and utilization efficiency, and making calcium compounds better deposited in bones, thereby enhancing bone density; bone collagen peptide powder can provide the required raw materials for cartilage renewal and repair; fish collagen peptide has a certain anti-inflammatory effect, can reduce joint inflammatory reactions, and relieve joint discomfort by inhibiting inflammation, and improve joint flexibility and stability; cherry powder has the function of promoting multiple complex proteins, N -Acetyl glucosamine absorption; N - Acetyl glucosamine is an important raw material for chondrocytes to synthesize proteoglycans and collagen fibers. It can stimulate the proliferation and differentiation of chondrocytes, promote chondrocytes to produce proteoglycans with normal polymer structure, increase the repair ability of chondrocytes, help repair damaged or worn cartilage, and maintain the normal structure and function of cartilage; Pueraria root powder has the effect of promoting the synthesis of multiple complex proteins, N-Acetyl glucosamine absorption effect; calcium compounds are an important component of bones and can provide raw materials for bone renewal and repair; curcumin in turmeric is a powerful natural anti-inflammatory substance that can effectively reduce bone damage by inhibiting inflammatory responses, thereby maintaining the normal structure and function of bones.

[0057] The beneficial effects of the present invention are:

[0058] 1) This invention combines multiple complex proteins (ray fish collagen peptide, bone collagen peptide powder, fish collagen peptide), cherry powder, N -Acetyl glucosamine, calcium compounds, kudzu root powder, and turmeric are prepared according to scientific proportions to prepare a composition that can promote cartilage repair and maintain joint health.

[0059] 2) The present invention adopts a special process to prepare cherry powder and kudzu root powder, which can be combined with the other components of the present invention to better promote the production of multiple complex proteins, N -The absorption of acetyl glucosamine can promote cartilage repair. DETAILED DESCRIPTION

[0060] In order to make the technical means, creative features, purpose and efficacy of the present invention easy to understand, the present invention is further illustrated below in conjunction with specific examples, but the following examples are only preferred embodiments of the present invention, not all. Based on the examples in the implementation manner, other embodiments obtained by those skilled in the art without making creative work are all within the scope of protection of the present invention. In the following examples, unless otherwise specified, the operating methods used are all conventional operating methods, the equipment used are all conventional equipment, and the equipment and materials used in each embodiment are all the same.

[0061] The sour cherries used in the following examples are Montmorency sour cherries.

[0062] Example 1 Preparation of composition

[0063] The composition comprises, by weight, a multi-protein complex (1 part of ray collagen peptide (purchased from Shanghai Kaida Biotechnology Co., Ltd.), 14 parts of bone collagen peptide powder, 36 parts of fish collagen peptide), 1 part of cherry powder, N -1 part of acetylglucosamine, 1.5 parts of kudzu root powder, 1.5 parts of calcium compound (the mass ratio of calcium citrate and calcium gluconate is 2:1), and 0.1 part of turmeric.

[0064] The particle sizes of the multiple protein complexes are as follows: 60-200 mesh accounts for 60%, 200-800 mesh accounts for 20%, and 800-2000 mesh accounts for 20%.

[0065] The preparation method of the composition is:

[0066] (1) Grind and mix the ray collagen peptide, bone collagen peptide powder and fish collagen peptide to obtain a multi-protein complex;

[0067] (2) Mix cherry powder, kudzu root powder and turmeric to obtain mixture 1;

[0068] (3) Add the multi-protein complex to mixture 1, then add N -acetylglucosamine and a calcium compound to obtain a composition.

[0069] The preparation of cherry powder comprises the following steps:

[0070] (1) Add 5 times the amount of water to the cherry to squeeze the juice, and pass it through a 40-mesh sieve to obtain the cherry juice;

[0071] (2) Add cellulase to the cherry juice puree for enzymatic hydrolysis, the material-liquid ratio of enzyme to cherry juice puree is 2.5:1, the enzymatic hydrolysis time is 50 minutes, the enzymatic hydrolysis temperature is 25℃, the enzyme is inactivated at 90℃ for 8 minutes, vacuum concentration is performed at a vacuum degree of 0.07Mpa, the vacuum concentration temperature is 60℃, and the relative density is concentrated to 1.25-1.30, and vacuum drying is performed at a vacuum drying condition of 0.08Mpa and a temperature of 80℃. Dry until the water content of the extract is less than 3%, and grind to pass through a 60-mesh sieve.

[0072] The preparation method of kudzu root powder is as follows: peel the fresh kudzu root, add 8 times the amount of water and boil for 80 minutes, use a wall breaking machine to beat, filter, let the filtrate stand for 12 hours, discard the precipitate, dry the filtrate at 40°C for 5 hours, and grind to obtain kudzu root powder.

[0073] Example 2 Preparation of composition

[0074] The composition comprises, by weight, a multi-protein complex (0.1 parts of ray collagen peptide, 10 parts of bone collagen peptide powder, 30 parts of fish collagen peptide), 0.5 parts of cherry powder, N -0.1 parts of acetylglucosamine, 0.5 parts of kudzu root powder, 0.6 parts of calcium compound (the mass ratio of calcium citrate and calcium gluconate is 1:1), and 0.08 parts of turmeric.

[0075] The particle sizes of the multiple protein complexes are as follows: 60-200 mesh accounts for 90%, 200-800 mesh accounts for 5%, and 800-2000 mesh accounts for 5%.

[0076] The preparation method of the composition is the same as that of Example 1.

[0077] The preparation of cherry powder comprises the following steps:

[0078] (1) Add 3 times the amount of water to the cherry to squeeze the juice, and pass it through a 40-mesh sieve to obtain the cherry juice;

[0079] (2) Add cellulase to the cherry juice puree for enzymatic hydrolysis, the material-liquid ratio of enzyme to cherry juice puree is 1:1, the enzymatic hydrolysis time is 50 minutes, the enzymatic hydrolysis temperature is 25℃, the enzyme is inactivated at 90℃ for 8 minutes, vacuum concentration is performed, the vacuum degree is 0.07Mpa, the vacuum concentration temperature is 60℃, and the relative density is 1.25-1.30. Vacuum drying is performed, the vacuum drying conditions are 0.08Mpa, the temperature is 80℃, and the extract is dried to a moisture content of less than 3%. The extract is then crushed and passed through a 60-mesh sieve to obtain the extract.

[0080] The preparation method of kudzu root powder is as follows: peel fresh kudzu root, add 10 times the amount of water, boil for 100 minutes, beat, filter, let the filtrate stand for 12 hours, discard the precipitate, dry the filtrate at 40°C for 5 hours, and grind to obtain kudzu root powder.

[0081] Example 3 Preparation of composition

[0082] The composition comprises, by weight, a multi-protein complex (6 parts of ray collagen peptide, 20 parts of bone collagen peptide powder, 40 parts of fish collagen peptide), 1.5 parts of cherry powder, N -1.5 parts of acetylglucosamine, 1.5 parts of kudzu root powder, 1 part of calcium compound (the mass ratio of calcium citrate and calcium gluconate is 3:1), and 0.15 parts of turmeric.

[0083] The particle size ratio of the multiple protein complex is the same as that in Example 1.

[0084] The preparation method of the composition is the same as that of Example 1.

[0085] The preparation of cherry powder comprises the following steps:

[0086] (1) Add 5 times the amount of water to the cherry to squeeze the juice, and pass it through a 40-mesh sieve to obtain the cherry juice;

[0087] (2) Add cellulase to the cherry juice puree for enzymatic hydrolysis, the material-liquid ratio of enzyme to cherry juice puree is 3:1, the enzymatic hydrolysis time is 60 minutes, the enzymatic hydrolysis temperature is 35℃, the enzyme is inactivated at 90℃ for 8 minutes, vacuum concentration is performed, the vacuum degree is 0.07Mpa, the vacuum concentration temperature is 60℃, and the relative density is 1.25-1.30. Vacuum drying is performed under the conditions of 0.08Mpa and the temperature is 80℃. Dry until the water content of the extract is less than 3%, and grind through a 60-mesh sieve to obtain the extract.

[0088] The preparation method of Pueraria powder is the same as that in Example 1.

[0089] Example 4 Preparation of composition

[0090] The composition comprises, by weight: a multi-protein complex (10 parts of ray collagen peptide, 30 parts of bone collagen peptide powder, 50 parts of fish collagen peptide), 3 parts of cherry powder, N -2 parts of acetyl glucosamine, 2 parts of kudzu root powder, 1.5 parts of calcium compound (the mass ratio of calcium citrate and calcium gluconate is 2:1), and 0.2 parts of turmeric.

[0091] The particle size ratio of the multiple protein complex is the same as that in Example 1.

[0092] The preparation of the composition is the same as in Example 1.

[0093] The preparation of cherry powder and kudzu root powder is the same as in Example 1.

[0094] Comparative Example 1

[0095] The only difference from Example 1 is that cherry powder is not added to the composition, and the rest are the same. The composition in Comparative Example 1 comprises, by weight:

[0096] Multi-protein complex (1 part of ray collagen peptide, 14 parts of bone collagen peptide powder, 36 parts of fish collagen peptide), N -1 part of acetylglucosamine, 1.5 parts of kudzu root powder, 1.5 parts of calcium compound (the mass ratio of calcium citrate and calcium gluconate is 2:1), and 0.1 part of turmeric.

[0097] The particle size ratio of the multiple protein complex is the same as that in Example 1.

[0098] The preparation method of Pueraria powder is the same as that in Example 1.

[0099] The preparation method of the composition is:

[0100] (1) Grind and mix the ray collagen peptide, bone collagen peptide powder and fish collagen peptide to obtain a multi-protein complex;

[0101] (2) Mix kudzu root powder and turmeric to obtain mixture 1;

[0102] (3) Add the multi-protein complex to mixture 1, then add N -acetylglucosamine and a calcium compound to obtain a composition.

[0103] Comparative Example 2

[0104] The difference from Example 1 is that Pueraria root powder is not added to the composition, and the rest are the same. The composition in Comparative Example 2 includes, by weight: a multi-protein complex (1 part of skate collagen peptide, 14 parts of bone collagen peptide powder, 36 parts of fish collagen peptide), 1 part of cherry powder, N-1 part of acetylglucosamine, 1.5 parts of calcium compound (the mass ratio of calcium citrate and calcium gluconate is 2:1), and 0.1 part of turmeric.

[0105] The particle size ratio of the multiple protein complex is the same as that in Example 1.

[0106] The preparation method of cherry powder is the same as that in Example 1.

[0107] The preparation method of the composition is:

[0108] (1) Grind and mix the ray collagen peptide, bone collagen peptide powder and fish collagen peptide to obtain a multi-protein complex;

[0109] (2) Mix cherry powder and turmeric to obtain mixture 1;

[0110] (3) Add the multi-protein complex to mixture 1, then add N -acetylglucosamine and a calcium compound to obtain a composition.

[0111] Comparative Example 3

[0112] The difference from Example 1 is that cherry powder and kudzu root powder are not added to the composition, and the rest are the same. The composition in Comparative Example 3 includes, by weight, a multi-protein complex (1 part of skate collagen peptide, 14 parts of bone collagen peptide powder, and 36 parts of fish collagen peptide), N -1 part of acetylglucosamine, 1.5 parts of calcium compound (the mass ratio of calcium citrate and calcium gluconate is 2:1), and 0.1 part of turmeric.

[0113] The particle size ratio of the multiple protein complex is the same as that in Example 1.

[0114] The preparation method of the composition is:

[0115] (1) Grind and mix the ray collagen peptide, bone collagen peptide powder and fish collagen peptide to obtain a multi-protein complex;

[0116] (1) Add the multi-protein complex to turmeric, then add N -acetylglucosamine and a calcium compound to obtain a composition.

[0117] Comparative Example 4

[0118] The only difference from Example 1 is that the particle size proportions of the multiple protein complex are different. In Comparative Example 4, 60-200 mesh accounts for 40%, 200-800 mesh accounts for 40%, and 800-2000 mesh accounts for 20%.

[0119] Comparative Example 5

[0120] The only difference from Example 1 is that the skate collagen peptide is replaced with type II collagen peptide, and the rest are the same.

[0121] Experimental Example 1 Effect of the Composition on Cartilage Repair

[0122] 1 Reagents, consumables and instruments

[0123] Reagents and consumables: transgenic cartilage green fluorescent zebrafish Tg (Co12ala:eGFP) (purchased from Huante Biotechnology), methylcellulose (Sigma, CAS: 497-76-7), chondroitin sulfate A sodium salt (C758506-5g, Aladdin), dexamethasone (Source Leaf BR, 99%, S17003-5g).

[0124] Instruments: stereo microscope (MZ62, Mshot), microscope photography system (BX53, OLYMPUS, Japan).

[0125] 2 Experimental steps

[0126] Normally developed 2-3 dpf Tg(col2a1a:EGFP) transgenic cartilage fluorescent zebrafish embryos were randomly selected and placed in six-well culture plates, 30 per well. Without harming the embryos, the standard dilution water in the six-well plates was removed and 3 mL of the corresponding concentration of the composition was quickly added to each well. For all groups except the blank control group, 25 μM dexamethasone was added to establish a zebrafish cartilage injury model. Chondroitin sulfate (1 mg / mL) was used as a positive control. The plates were covered and wrapped with aluminum foil. The cells were incubated in a dark incubator at (28.5 ± 1)°C for 3 days. After 3 days, the green fluorescence intensity of the zebrafish was measured.

[0127] 2.1 Experimental Grouping

[0128] Blank control group: containing 2-3 dpf zebrafish embryos and 3 mL of standard dilution water;

[0129] Model control group: containing 2-3 dpf zebrafish embryos and 25 μM dexamethasone prepared in standard dilution water;

[0130] Positive control group: containing 2-3 dpf zebrafish embryos and 25 μM dexamethasone and 1 mg / mL chondroitin sulfate prepared in standard dilution water;

[0131] Example 1 group: containing 2-3 dpf zebrafish embryos, 25 μM dexamethasone prepared in standard dilution water, and 0.5 mg / mL of the composition prepared in Example 1;

[0132] Example 2 group: containing 2-3 dpf zebrafish embryos, 25 μM dexamethasone prepared in standard dilution water, and 0.5 mg / mL of the composition prepared in Example 2;

[0133] Example 3 group: containing 2-3 dpf zebrafish embryos, 25 μM dexamethasone prepared in standard dilution water, and 0.5 mg / mL of the composition prepared in Example 3;

[0134] Example 4 group: containing 2-3 dpf zebrafish embryos, 25 μM dexamethasone prepared in standard dilution water, and 0.5 mg / mL of the composition prepared in Example 4;

[0135] Comparative Example 1 group: containing 2-3 dpf zebrafish embryos, 25 μM dexamethasone prepared in standard dilution water, and 0.5 mg / mL of the composition prepared in Comparative Example 1;

[0136] Comparative Example 2 group: containing 2-3 dpf zebrafish embryos, 25 μM dexamethasone prepared in standard dilution water, and 0.5 mg / mL of the composition prepared in Comparative Example 2;

[0137] Comparative Example 3 group: containing 2-3 dpf zebrafish embryos, 25 μM dexamethasone prepared in standard dilution water, and 0.5 mg / mL of the composition prepared in Comparative Example 3;

[0138] Comparative Example 4 group: containing 2-3 dpf zebrafish embryos, 25 μM dexamethasone prepared in standard dilution water, and 0.5 mg / mL of the composition prepared in Comparative Example 4;

[0139] Comparative Example 5 group: containing 2-3 dpf zebrafish embryos, 25 μM dexamethasone prepared in standard dilution water, and 0.5 mg / mL of the composition prepared in Comparative Example 5.

[0140] 2.2 Observation, photography, and data analysis

[0141] Three days after treatment with the test substance, zebrafish from each experimental group were observed and photographed under a fluorescence microscope. At least 10 zebrafish were randomly selected from each experimental group and fixed with 3% methylcellulose. The green fluorescence of the zebrafish craniofacial cartilage was photographed and collected. Image J software was used to quantify the fluorescence intensity (A) of the zebrafish craniofacial cartilage. The statistical analysis results of this indicator were used to evaluate the ability of the composition to repair dexamethasone-induced cartilage damage. Statistical results are expressed as mean ± SE. Graphpad Prism software was used for plotting and statistical analysis. p <0.05 indicated that the difference was statistically significant.

[0142] The calculation formula for cartilage repair:

[0143] Cartilage regeneration rate C = (A1-A0) / A0×100%;

[0144] Where, C is the cartilage regeneration rate,%;

[0145] A0—fluorescence intensity of zebrafish craniofacial cartilage in the model control group (n=10);

[0146] A1—Fluorescence intensity of zebrafish craniofacial cartilage of each composition (n=10).

[0147] 3 Experimental Results

[0148] The cartilage EGFP fluorescence intensity and cartilage regeneration rate of zebrafish in each group are shown in Table 1 below:

[0149] Table 1 Cartilage EGFP fluorescence intensity and cartilage regeneration rate of zebrafish in each group

[0150]

[0151] Note: Compared with the blank control group, *p<0.05; compared with the model control group, #p<0.05.

[0152] As can be seen from the above table, compared with the blank control group, the fluorescence intensity of the cartilage in the model control group was significantly reduced. Compared with the model control group, the cartilage regeneration rates of the compositions prepared in Examples 1-4 of the present application were 35.3%, 34.8%, 32.3% and 34.2%, respectively, indicating that the compositions of the present application have a good effect in promoting cartilage regeneration. Comparative Examples 1-3 lack one or two components, respectively, and their effect on promoting cartilage regeneration is significantly reduced. The particle size ratio of the multiple protein complex in Comparative Example 4 and the replacement of the ray collagen peptide with type II collagen peptide in Comparative Example 5 make its effect on cartilage regeneration significantly lower than that of the Example 1-4 group.

[0153] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A composition for promoting cartilage repair and maintaining joint health, characterized in that: The composition is made of the following components in parts by weight: 21.1-90 parts of multi-protein complex, 0.1-3 parts of cherry powder, N - 0.01-2 parts of acetylglucosamine, 0.01-2 parts of kudzu root powder, 0.1-1.5 parts of calcium compound, 0.01-0.2 parts of turmeric; The multi-protein complex is composed of 0.1-10 parts of ray collagen peptide, 1-30 parts of bone collagen peptide powder, and 20-50 parts of fish collagen peptide; the particle size of the multi-protein complex is as follows: 60-200 mesh accounts for 50-90%, 200-800 mesh accounts for 5-30%, and 800-2000 mesh accounts for 1-20%; The preparation method of the cherry powder is: (1) adding 3-5 times the amount of water to the cherries to squeeze out the juice to obtain cherry juice; the cherries are sour cherries; (2) Adding enzyme to the cherry juice to perform enzymolysis, inactivate the enzyme, concentrate, and dry to obtain cherry powder; The enzyme used in the enzymatic hydrolysis of step (2) is selected from one or more of cellulase, pectinase, and hemicellulase; the material-liquid ratio of enzyme to cherry juice puree is (1-3):1; the enzymatic hydrolysis time is 40-60 minutes; the enzymatic hydrolysis temperature is 25-35°C; The preparation method of the kudzu root powder comprises the following steps: adding water to the kudzu root, boiling the kudzu root, beating the kudzu root, filtering, discarding the precipitate, and drying the filtrate to obtain the kudzu root powder; and the boiling time is 60-100 minutes.

2. The composition according to claim 1, characterized in that The composition is made of the following components in parts by weight: 0.1-6 parts of ray collagen peptide, 10-20 parts of bone collagen peptide powder, 30-40 parts of fish collagen peptide, 0.5-1.5 parts of cherry powder, N - 0.1-1.5 parts of acetylglucosamine, 0.5-1.5 parts of kudzu root powder, 0.6-1 parts of calcium compound, and 0.08-0.15 parts of turmeric.

3. The method for preparing the composition according to any one of claims 1 to 2, characterized in that: The following steps are involved: (1) Grind and mix the ray collagen peptide, bone collagen peptide powder and fish collagen peptide to obtain a multi-protein complex; (2) Mix cherry powder, kudzu root powder and turmeric to obtain mixture 1; (3) Add mixture 1 to the multi-protein complex, and then add N -acetylglucosamine and a calcium compound to obtain a composition.

4. Use of the composition according to any one of claims 1 to 2 in the preparation of a product for promoting cartilage repair.

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