Method for bacteriostasis and deodorization of livestock and poultry farm

Abstract

The invention belongs to the technical field of animal breeding, and particularly relates to a method for bacteriostasis and deodorization in a livestock and poultry farm, which is characterized in that subacid electrolyzed water and bacillus subtilis are combined for bacteriostasis and deodorization; the subacid electrolyzed water comprises subacid electrolyzed water I and subacid electrolyzed water II; the effective chlorine concentration in the first subacid electrolyzed water is 150-250 mg / L, the usage amount of the first subacid electrolyzed water is 150-325 mL / m < 2 >, the effective chlorine concentration in the second subacid electrolyzed water is 40-70 mg / L, and the usage amount of the second subacid electrolyzed water is 150-325 mL / m < 2 >; the bacillus subtilis is used after the subacid electrolyzed water I is used, and the viable count of the bacillus subtilis is not less than 10 < 6 > CFU / m < 2 >. The broad-spectrum bactericidal characteristic of the SAEW and the microbial ecological regulation effect of the bacillus subtilis are reasonably utilized, so that the microorganisms in the livestock and poultry farm environment can keep better microbial community ecology.

Description

Technical Field

[0001] The present invention relates to the technical field of animal breeding, and particularly relates to a method for inhibiting bacteria and deodorizing in livestock and poultry farms. Background Art

[0002] Disease is one of the important factors causing economic losses in livestock and poultry farms. There are many measures for the prevention and control of diseases. Disinfection is one of the important measures to reduce pathogenic microorganisms and lower the incidence of diseases. However, the use of some chemical disinfectants often brings problems of drug residues. Slightly acidic electrolyzed water (SAEW) is a cheap and safe disinfectant with instantaneous and high-efficiency bactericidal effects on various bacteria, fungi and viruses. It is obtained by electrolyzing dilute salt and / or dilute hydrochloric acid solution, with a pH value approximately neutral, about 5.0 - 6.5. After sterilization, it can be reduced to ordinary water, so it has low harm to the environment and human body. Currently, it has been popularized and applied in livestock and poultry farms. However, this disinfectant is broad-spectrum antibacterial. Long-term use will inhibit the growth of beneficial bacteria in the livestock and poultry environment, resulting in the disorder of the microbial ecosystem in the livestock and poultry farm, reducing the resistance to pathogenic bacteria. The livestock and poultry farm can only continuously use disinfectants to inhibit pathogenic bacteria, which is not conducive to the long-term ecological prevention and control of the livestock and poultry farm. Therefore, how to promote the development of the microbial ecosystem in the livestock and poultry farm while preventing and controlling diseases is the bottleneck for solving the long-term biosafety of the livestock and poultry farm.

[0003] Bacillus subtilis (B. subtilis) is a beneficial bacterium with strong stress resistance. It not only has antagonistic effects on Escherichia coli, Salmonella, Staphylococcus aureus, molds, etc., but also can compete for the nutrient sites of pathogenic bacteria, maintain the microecological safety of the environment, and at the same time has the functions of deodorizing and eliminating harmful gases. However, in previous research arguments, because chlorine preparations such as SAEW are broad-spectrum bactericides and also have inhibitory effects on Bacillus subtilis, it is difficult to agree with the argument of combining Bacillus subtilis with disinfectants. Summary of the Invention

[0004] If chlorine preparations such as SAEW and Bacillus subtilis are to be used in combination, the following problems need to be solved: (1) It is necessary to reduce the influence of SAEW on B. subtilis and at the same time increase the influence of SAEW on pathogenic bacteria; this requires finding the appropriate action time or concentration of SAEW. (2) If it is to be helpful enough for restoring the microecological environment of the livestock farm, it is necessary to strive to improve the dominant position of B. subtilis among other microorganisms, especially among the microorganisms with competitive interspecies relationships. The solution of the above problems can realize the combined use of disinfectants and microbial live preparations, promote the reasonable use of disinfectants in livestock and poultry farms, specifically inhibit pathogenic bacteria, and at the same time promote the growth of beneficial bacteria and improve the microbial environmental ecosystem of livestock and poultry farms.

[0005] The present invention provides a method for antibacterial and deodorizing in livestock and poultry farms, which combines slightly acidic electrolyzed water and Bacillus subtilis for antibacterial and deodorizing;

[0006] The slightly acidic electrolyzed water includes slightly acidic electrolyzed water one (SAEW1) and slightly acidic electrolyzed water two (SAEW2);

[0007] In the slightly acidic electrolyzed water one, the available chlorine concentration (ACC) is 150 - 250 mg / L, and the usage amount is 150 - 325 mL / m 2 , in the slightly acidic electrolyzed water two, the available chlorine concentration is 40 - 70 mg / L, and the usage amount is 150 - 325 mL / m 2 ;

[0008] After using the slightly acidic electrolyzed water one, use the Bacillus subtilis, and the viable count of the Bacillus subtilis is not less than 10 6 CFU / m 2 .

[0009] It should be noted that, for cost consideration, the viable count of the Bacillus subtilis is 10 6 CFU / m 2 .

[0010] Preferably, in the slightly acidic electrolyzed water one, the ORP (oxidation-reduction potential) is 700 - 900 mv and the pH is 5.0 - 7.0.

[0011] Preferably, in the slightly acidic electrolyzed water two, the ORP is 600 - 700 mv and the pH is 5.0 - 7.0.

[0012] Preferably, the concentration of the Bacillus subtilis bacterial liquid is above 10 7 CFU / mL.

[0013] It should be noted that, for cost consideration, the concentration of the Bacillus subtilis bacterial liquid is 10 7 CFU / mL.

[0014] Preferably, the volume of the Bacillus subtilis bacterial liquid is 8 - 10 mL.

[0015] Preferably, it includes the following steps:

[0016] Use the slightly acidic electrolyzed water one for disinfection one;

[0017] After disinfection one, wait for air drying and then spray the bacterial liquid of Bacillus subtilis;

[0018] After spraying, use the slightly acidic electrolyzed water two for disinfection two.

[0019] Preferably, the time for disinfection one is 12 - 20 min.

[0020] Preferably, the bacterial solution of Bacillus subtilis is sprayed 2 times a day for 3 days.

[0021] Preferably, the disinfection II is carried out once a day for 3 days.

[0022] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0023] 1. In the present invention, SAEW is an environment-friendly disinfectant, and Bacillus subtilis is a harmless and safe microorganism. The present invention rationally utilizes the broad-spectrum bactericidal property of SAEW and the microbial ecological regulation function of Bacillus subtilis, so that the microorganisms in the livestock and poultry farm environment can maintain a better microbial community ecology.

[0024] 2. The product of the present invention has a good effect of inhibiting pathogenic microorganisms and deodorizing, can effectively inhibit the harmful pathogenic bacteria in the breeding farm environment, and at the same time improve the community level of beneficial bacteria. The present invention has proved the antibacterial and deodorizing effects of the product through antibacterial tests and ammonia and hydrogen sulfide removal tests.

[0025] 3. In the present invention, no chemical disinfectant is used, and there is no problem of harm to the user's body caused by chemical residues, which meets the requirements of the current development of the breeding industry.

[0026] In summary, the present invention combines Bacillus subtilis with the environment-friendly disinfectant SAEW, making its antibacterial and deodorizing effects stronger. At the same time, the product is safe and pollution-free, can improve the microbial environment ecology of livestock and poultry farms, meets the requirements of modern breeding industry, and has a broad market prospect. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 It is a diagram for explaining the technical steps and principles of the present invention;

[0028] Figure 2 It is the inactivation effect of slightly acidic electrolyzed water with different available chlorine concentrations on Bacillus subtilis. Note that different letters in the figure indicate significant differences (P < 0.05);

[0029] Figure 3 It is the inactivation effect of 70 mg / L slightly acidic electrolyzed water on Salmonella enteritidis, Staphylococcus aureus and Escherichia coli. Note that different letters in the figure indicate significant differences (P < 0.05);

[0030] Figure 4Changes in the number of bacteria during the co - culture of Bacillus subtilis and Salmonella. Note: S.enteritidis+SAEW: The number of Salmonella after co - culture of Bacillus subtilis and Salmonella after the action of SAEW; B.subtilis+SAEW: The number of Bacillus subtilis after co - culture of Bacillus subtilis and Salmonella after the action of SAEW; B.subtilis: The number of Bacillus subtilis after co - culture of Bacillus subtilis and Salmonella without the action of SAEW; S.enteritidis: The number of Salmonella after co - culture of Bacillus subtilis and Salmonella without the action of SAEW; Different letters in the figure indicate significant differences (P < 0.05).

[0031] Figure 5 It is the inhibitory effect of SAEW combined with B.subtilis on bacteria in the chicken house. Among them, A is Escherichia coli, B is Salmonella, C is Staphylococcus, and D is Bacillus subtilis. Specific implementation mode

[0032] The following is a detailed description of the specific implementation mode of the present invention, but it should be understood that the protection scope of the present invention is not limited by the specific implementation mode. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative work belong to the protection scope of the present invention. The experimental methods described in the embodiments of the present invention are all conventional methods unless otherwise specified.

[0033] Bacillus subtilis is sourced from Beijing Baina Biotechnology Co., Ltd.

[0034] Salmonella is sourced from Beijing Baina Biotechnology Co., Ltd.

[0035] Example 1

[0036] A method for inhibiting bacteria and deodorizing in livestock and poultry farms, characterized by including the following steps:

[0037] (1) Use slightly acidic electrolyzed water 1 with a concentration of 150 mg / L to disinfect the environment. The usage amount of slightly acidic electrolyzed water 1 is 200 mL / m 2 , and the disinfection is carried out for 12 min to completely disinfect the livestock and poultry farm. After the disinfection is completed, ventilate for 30 min until the walls in the house are dry. The ORP of the slightly acidic electrolyzed water 1 is 700 mv and the pH is 5.0;

[0038] (2) After air - drying in step (1), spray 8 mL of a bacterial liquid of Bacillus subtilis with a concentration of 10 7 CFU / mL. At this time, the viable bacteria count is 10 6 CFU / m 2 , spray 2 times a day, once in the morning and once in the evening, and continuously spray for 3 d;

[0039] (3) After step (2), disinfect the environment with slightly acidic electrolyzed water II with a concentration of 40 mg / L. The usage amount of slightly acidic electrolyzed water II is 200 mL / m 2 , and spray once a day for 3 days. The ORP of the slightly acidic electrolyzed water II is 600 mv and the pH is 5.0.

[0040] Example 2

[0041] A method for inhibiting bacteria and removing odor in a livestock and poultry farm, characterized by comprising the following steps:

[0042] (1) Disinfect the environment with slightly acidic electrolyzed water I with a concentration of 200 mg / L. The usage amount of slightly acidic electrolyzed water I is 150 mL / m 2 , and conduct disinfection for 15 minutes to completely disinfect the livestock and poultry farm. After the disinfection is completed, ventilate for 35 minutes until the walls in the shed are dry. The ORP of the slightly acidic electrolyzed water I is 800 mv and the pH is 6.0;

[0043] (2) After air-drying in step (1), spray 9 mL of a bacterial solution of Bacillus subtilis with a concentration of 10 8 CFU / mL. At this time, the viable bacteria count is 10 7 CFU / m 2 , and spray twice a day, once in the morning and once in the evening, for 3 consecutive days;

[0044] (3) After step (2), disinfect the environment with slightly acidic electrolyzed water II with a concentration of 50 mg / L. The usage amount of slightly acidic electrolyzed water II is 150 mL / m 2 , and spray once a day for 3 days. The ORP of the slightly acidic electrolyzed water II is 650 mv and the pH is 6.0.

[0045] Example 3

[0046] A method for inhibiting bacteria and removing odor in a livestock and poultry farm, characterized by comprising the following steps:

[0047] (1) Disinfect the environment with slightly acidic electrolyzed water I with a concentration of 250 mg / L. The usage amount of slightly acidic electrolyzed water I is 325 mL / m 2 , and conduct disinfection for 20 minutes to completely disinfect the livestock and poultry farm. After the disinfection is completed, ventilate for 40 minutes until the walls in the shed are dry;

[0048] (2) After air-drying in step (1), spray 10 mL of a bacterial solution of Bacillus subtilis with a concentration of 10 7 CFU / mL. At this time, the viable bacteria count is 10 7 CFU / m 2, spray 2 times a day, once in the morning and once in the evening, and continue spraying for 3 days;

[0049] (3) After step (2), disinfect the environment with microacidic electrolyzed water II with a concentration of 70 mg / L. The usage amount of microacidic electrolyzed water II is 325 mL / m 2 , spray 1 time a day for 3 days. The ORP of the microacidic electrolyzed water II is 700 mv and the pH is 7.0.

[0050] Comparative Example 1

[0051] A method for antibacterial and deodorizing in a livestock and poultry farm, characterized by including the following steps:

[0052] (1) Disinfect the environment with microacidic electrolyzed water I with a concentration of 100 mg / L. The usage amount of microacidic electrolyzed water I is 200 mL / m 2 , carry out disinfection I for 15 minutes to completely disinfect the livestock and poultry farm. After disinfection I is completed, ventilate for at least 30 minutes until the inner walls of the shed are dry. The ORP of the microacidic electrolyzed water I is 700 mv and the pH is 5.0;

[0053] (2) After air drying in step (1), spray 8 mL of a bacterial liquid of Bacillus subtilis with a concentration of 10 7 CFU / mL. At this time, the viable bacteria count is 10 6 CFU / m 2 , spray 2 times a day, once in the morning and once in the evening, and continue spraying for 3 days;

[0054] (3) After step (2), disinfect the environment with microacidic electrolyzed water II with a concentration of 70 mg / L. The usage amount of microacidic electrolyzed water II is 200 mL / m 2 , spray 1 time a day for 3 days. The ORP of the microacidic electrolyzed water II is 600 mv and the pH is 5.0.

[0055] Comparative Example 2

[0056] A method for antibacterial and deodorizing in a livestock and poultry farm, characterized by including the following steps:

[0057] (1) Disinfect the environment with microacidic electrolyzed water I with a concentration of 300 mg / L. The usage amount of microacidic electrolyzed water I is 200 mL / m 2 , carry out disinfection I for 15 minutes to completely disinfect the livestock and poultry farm. After disinfection I is completed, ventilate for 30 minutes until the inner walls of the shed are dry. The ORP of the microacidic electrolyzed water I is 700 mv and the pH is 5.0;

[0058] (2) After air drying in step (1), spray 8 mL of a concentration of 10 7A bacterial solution of Bacillus subtilis at 10 CFU / mL, with a viable count of 10 6 CFU / m 2 , sprayed 2 times a day, once in the morning and once in the evening, continuously sprayed for 3 days;

[0059] (3) After step (2), disinfect the environment with slightly acidic electrolyzed water II at a concentration of 70 mg / L, and the usage amount of slightly acidic electrolyzed water II is 200 mL / m 2 , sprayed 1 time a day, continuously for 3 days, and the ORP of the slightly acidic electrolyzed water II is 600 mv and the pH is 5.0.

[0060] Comparative Example 3

[0061] A method for inhibiting bacteria and removing odors in a livestock and poultry farm, characterized by comprising the following steps:

[0062] (1) Disinfect the environment with slightly acidic electrolyzed water I at a concentration of 150 mg / L, and the usage amount of slightly acidic electrolyzed water I is 200 mL / m 2 , disinfect for 15 minutes to completely disinfect the livestock and poultry farm. After the disinfection is completed, ventilate for 30 minutes until the walls in the shed are dry. The ORP of the slightly acidic electrolyzed water I is 700 mv and the pH is 5.0;

[0063] (2) After air-drying in step (1), spray 8 mL of a bacterial solution of Bacillus subtilis at a concentration of 10 7 CFU / mL, with a viable count of 10 6 CFU / m 2 , sprayed 2 times a day, once in the morning and once in the evening, continuously sprayed for 3 days;

[0064] (3) After step (2), disinfect the environment with slightly acidic electrolyzed water II at a concentration of 30 mg / L, and the usage amount of slightly acidic electrolyzed water II is 200 mL / m 2 , sprayed 1 time a day, continuously for 3 days, and the ORP of the slightly acidic electrolyzed water II is 600 mv and the pH is 5.0.

[0065] Comparative Example 4

[0066] A method for inhibiting bacteria and removing odors in a livestock and poultry farm, characterized by comprising the following steps:

[0067] (1) Disinfect the environment with slightly acidic electrolyzed water I at a concentration of 150 mg / L, and the usage amount of slightly acidic electrolyzed water I is 200 mL / m 2 , disinfect for 15 minutes to completely disinfect the livestock and poultry farm. After the disinfection is completed, ventilate for 30 minutes until the walls in the shed are dry. The ORP of the slightly acidic electrolyzed water I is 700 mv and the pH is 5.0;

[0068] (2) After air-drying in step (1), spray 8 mL of a bacterial solution of Bacillus subtilis with a concentration of 10 7 CFU / mL. At this time, the viable count is 10 6 CFU / m 2 , spray 2 times a day, once in the morning and once in the evening, and continue spraying for 3 days;

[0069] (3) After step (2), use slightly acidic electrolyzed water II with a concentration of 80 mg / L to completely spray and disinfect the environment. Spray once a day for 3 days. The ORP of the slightly acidic electrolyzed water II is 600 mV and the pH is 5.0.

[0070] Comparative Example 5

[0071] A method for inhibiting bacteria and deodorizing in a livestock and poultry farm, characterized by including the following steps:

[0072] (1) Use slightly acidic electrolyzed water I with a concentration of 200 mg / L to disinfect the environment once. The usage amount of slightly acidic electrolyzed water I is 200 mL / m 2 , conduct disinfection once for 15 minutes to completely disinfect the livestock and poultry farm. After the disinfection is completed, ventilate for 35 minutes until the inner walls of the house are air-dried. The ORP of the slightly acidic electrolyzed water I is 800 mV and the pH is 6.0;

[0073] (2) After air-drying in step (1), spray 7 mL of a bacterial solution of Bacillus subtilis with a concentration of 10 7 CFU / mL. At this time, the viable count is 10 5 CFU / m 2 , spray 2 times a day, once in the morning and once in the evening, and continue spraying for 3 days;

[0074] (3) After step (2), use slightly acidic electrolyzed water II with a concentration of 70 mg / L to disinfect the environment twice. The usage amount of slightly acidic electrolyzed water II is 200 mL / m 2 , spray once each time for 3 days. The ORP of the slightly acidic electrolyzed water II is 700 mV and the pH is 7.0.

[0075] Effect verification

[0076] 1. After treatment with Examples 1 - 3 and Comparative Examples 1 - 5, analyze the inhibitory effect of SAEW combined with B. subtilis on bacteria in the chicken house. The results are shown in Table 1.

[0077] Table 1 Analysis of the inhibitory effect of SAEW combined with B. subtilis (under different parameters) on bacteria in the chicken house.

[0078]

[0079] Note: Different letters in the same column in the table indicate significant differences (P < 0.05).

[0080] As shown in Table 1, the detection rates of pathogenic bacteria in Examples 1, 2, and 3 were significantly lower than those in Comparative Example 1, Comparative Example 3, and Comparative Example 4. This indicates the necessity of selecting the ACC range for slightly acidic electrolyzed water I and slightly acidic electrolyzed water II. There was no significant difference in the detection rate of pathogenic bacteria between Examples 1, 2, and 3 and Comparative Example 5. However, the viable count of Bacillus subtilis in Examples 1, 2, and 3 was significantly higher than that in Comparative Example 5. There was no significant difference in the detection rate of pathogenic bacteria and the viable count of Bacillus subtilis between Examples 1, 2, and 3 and Comparative Example 2. However, there were significant increases in the available chlorine concentration of slightly acidic electrolyzed water I and the viable count of Bacillus subtilis in Comparative Example 2 and Comparative Example 5, respectively. From the perspective of cost input, Examples 1 - 3 were significantly lower than Comparative Example 2 and Comparative Example 5.

[0081] In summary, through the above analysis, it is shown that the available chlorine concentration in slightly acidic electrolyzed water I should be 150 - 250 mg / L, the available chlorine concentration in slightly acidic electrolyzed water II should be 40 - 70 mg / L, and the viable count of Bacillus subtilis should not be less than 10 6 CFU / m 2 .

[0082] 2. Antibacterial test of Bacillus subtilis against Escherichia coli, Salmonella, and Staphylococcus aureus under the action of SAEW

[0083] (1) Changes in the number of Bacillus subtilis under the action of SAEW with different available chlorine concentrations under the interference of 0.5% bovine serum albumin

[0084] Using an SAEW machine, SAEW with available chlorine concentrations of 40, 70, and 100 mg / L was prepared respectively.

[0085] B. subtilis: BNCC109047, activate Bacillus subtilis according to the instructions and perform colony counting to obtain an original bacterial liquid concentration of more than 10 7 CFU / mL. Treat Bacillus subtilis with SAEW of different available chlorine concentrations for 2 min, that is: mix 0.5 mL of B. subtilis with 0.5 mL of bovine serum albumin, then add it to a 9 mL test tube containing SAEW, shake well, after 1.5 min, transfer 1 mL and add it to a 9 mL stop solution (Na2S2O3), then perform ten-fold dilution and count according to the microbial counting test method. Finally, place it in an incubator for 24 h of culture and perform manual counting. The results are shown in Figure 2 .

[0086] From Figure 2It can be seen that when there is organic matter interference and at a relatively low available chlorine concentration, the inhibitory effect of SAEW on B. subtilis is not obvious. According to Figure 2 the results, SAEW with an ACC of 70 mg / L was selected for the next experiment.

[0087] (2) Bacteriostatic effects of SAEW with an ACC of 70 mg / L on Escherichia coli, Salmonella and Staphylococcus aureus

[0088] Staphylococcus aureus, Escherichia coli and Salmonella were activated according to the instructions, and colony counting was carried out to obtain an original bacterial suspension concentration of more than 10 7 CFU / mL. The test for the inhibitory effect of SAEW on the bacterial suspension referred to the method in (1). The results are shown in Figure 3 .

[0089] From Figure 3 it can be seen that when the ACC is 70 mg / L, SAEW has a significant bacteriostatic effect on Escherichia coli, Salmonella and Staphylococcus aureus. Combining the test results in (1), it can be concluded that when the ACC of SAEW is 70 mg / L, it has no significant effect on B. subtilis (P > 0.05), but has a significant effect on some pathogenic bacteria (P < 0.05).

[0090] (3) Changes in the number of bacteria after co-culturing B. subtilis and Salmonella under the action of SAEW with an ACC of 70 mg / L

[0091] 1 mL each of the activated B. subtilis and Salmonella were taken, mixed evenly, and then processed according to the method in (1), and separated and counted with different selective media. The results are shown in Figure 4 .

[0092] From Figure 4 it can be seen that when treated with SAEW, when B. subtilis and Salmonella are co-cultured together, compared with the group without SAEW treatment, it has a significant advantage, and its bacterial count increases significantly (P < 0.05), while Salmonella decreases significantly (P < 0.05). It is proved that the treatment with SAEW with low ACC makes B. subtilis dominant in the microbial environment.

[0093] 3. Inhibitory effect of SAEW combined with B. subtilis on bacteria in chicken houses

[0094] First, SAEW with high ACC, namely slightly acidic electrolyzed water 1, is used for broad-spectrum and highly efficient sterilization to remove most microorganisms in the environment. Subsequently, B. subtilis is sprayed to establish the dominant ecological niche of B. subtilis by using the "microbial priority effect". Finally, SAEW with low ACC, namely slightly acidic electrolyzed water 2, is sprayed to inhibit pathogenic bacteria without affecting B. subtilis, further increasing the dominant position of B. subtilis for the long-term establishment and repair of the microecology in the livestock farm environment.

[0095] Three chicken coops are selected and treated in different ways. The treatment methods are: untreated group, SAEW group, B. subtilis group, and SAEW + B. subtilis group. Specifically:

[0096] SAEW treatment group: SAEW with high ACC (250 mg / L) is used for disinfection, and the usage amount is 200 mL / m 2 , and after drying and ventilation for 30 min, when the walls in the coop are dry, SAEW with an available chlorine concentration of 70 mg / L is used for complete spraying disinfection, and the usage amount is 200 mL / m 2 , lasting for 6 days.

[0097] B. subtilis group: Bacillus subtilis is sprayed alone, and 8 mL of a Bacillus subtilis bacterial solution with a concentration of 10 7 CFU / mL (10 6 CFU / m 2 ) is sprayed, twice a day, lasting for 6 days.

[0098] SAEW + B. subtilis group: First, SAEW with an available chlorine concentration of 250 mg / L is used to disinfect the environment, and the usage amount is 200 mL / m 2 . After disinfection is completed, it is dried and ventilated for 30 min. When the walls in the coop are dry, 8 mL of Bacillus subtilis with a concentration of 10 7 CFU / mL (10 6 CFU / m 2 ) is sprayed on the walls and the ground, twice a day, once in the morning and once in the evening. After continuous spraying for 3 days, SAEW with an available chlorine concentration of 70 mg / L is used for disinfection, and the usage amount is 200 mL / m 2 , once a day, lasting for 3 days.

[0099] Before and after disinfection, the wiping method was used to sample the surfaces of the walls, floors, and feeding troughs in the chicken house. It was divided into 3 replicates, and 20 samples were collected for each replicate. The sampling method was as follows: Use a sterile medical cotton swab moistened with peptone buffer to wipe back and forth about 20 times at the sampling point, and use sterilized forceps to break the cotton swab head into a centrifuge tube containing buffer. The collected samples were stored at low temperature and brought into the laboratory for microbial counting tests. Selective media were used to isolate the flora, brilliant green agar was used to isolate Salmonella, Chapman stone agar was used to isolate Staphylococcus, and MacConkey agar was used to isolate Escherichia coli group. Subsequently, a microscope was used to observe the colony morphology.

[0100] The bacterial detection rate was calculated by dividing the number of positive samples of the bacteria detected by the number of samples, and finally the average value and standard deviation were calculated. The results are shown in Figure 5 .

[0101] It can be seen from Figure 5 that by adopting this method, compared with other single treatment groups, the detection rates of pathogenic bacteria such as Salmonella, Staphylococcus, and Escherichia coli in the SAEW + B. subtilis group were significantly reduced (P < 0.05). In addition, there was no significant difference in the viable count of B. subtilis between the SAEW + B. subtilis group and the group treated with B. subtilis alone (P > 0.05). It was proved that this method could not only inhibit the growth of pathogenic bacteria, but also had no significant effect on beneficial bacteria such as B. subtilis. It could repair the microbial ecology of the livestock and poultry farm environment while disinfecting.

[0102] 4. Removal effect of SAEW combined with Bacillus subtilis on harmful gases in the chicken house

[0103] On the basis of 3, the changes in ammonia and hydrogen sulfide concentrations in the livestock house before and after treatment were measured.

[0104] Before the test, the feces in the house were cleaned up first, and then the doors and windows of the chicken house were closed for 30 min. A stench gas rapid detector was used to measure ammonia and hydrogen sulfide in the house. Subsequently, the method of the SAEW + B. subtilis group in 2 was used to carefully treat the ground, walls, cages, etc. of the chicken house and wait for the house to dry. The chicken house was also sealed during the treatment, and the doors and windows were closed. Before and after the treatment, a stench gas rapid detector was used to measure ammonia and hydrogen sulfide in the house, and then calculations were made to obtain that the removal rates of ammonia and hydrogen sulfide before treatment were 13 mg / m 3 and 0.08 mg / m 3 , and after removal were 0.5 mg / m 3 and 0.0024 mg / m 3 , and their removal rates were 96.2% and 97% respectively.

[0105] In summary, the present invention first uses SAEW with a high ACC for complete disinfection. After the environment is purified, B. subtilis is sprayed, and then SAEW with a low ACC is used. By virtue of the broad-spectrum and high-efficiency of SAEW with a high ACC and the different resistance performances of various bacteria to SAEW with a low ACC, a method for enhancing the dominant position of beneficial bacteria in the microbial population is developed. This method is a safe and environmentally friendly disinfection and deodorization method, which can not only sterilize and deodorize, but also increase the beneficial microorganisms in the livestock and poultry farms to repair the environmental microecology of the livestock and poultry farms.

[0106] It should be noted that when the claims of the present invention involve numerical ranges, it should be understood that any one of the two endpoints of each numerical range and any value between the two endpoints can be selected. To avoid redundancy, the preferred embodiments of the present invention are described.

[0107] Although the preferred embodiments of the present invention have been described, those skilled in the art can make additional changes and modifications to these embodiments once they know the basic creative concept. Therefore, the appended claims are intended to be construed as including the preferred embodiments and all changes and modifications falling within the scope of the present invention.

[0108] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalent technologies, the present invention also intends to include these modifications and variations.

Claims

1. A method for antibacterial and deodorizing in livestock and poultry farms, characterized in that, Combining slightly acidic electrolyzed water and Bacillus subtilis for antibacterial and deodorizing; The slightly acidic electrolyzed water includes slightly acidic electrolyzed water I and slightly acidic electrolyzed water II; The effective chlorine concentration in the slightly acidic electrolyzed water I is 150 - 250 mg / L, and the usage amount is 150 - 325 mL / m 2 , the effective chlorine concentration in the slightly acidic electrolyzed water II is 40 - 70 mg / L, and the usage amount is 150 - 325 mL / m 2 ; Use the Bacillus subtilis after using the slightly acidic electrolyzed water I, and the viable count of the Bacillus subtilis is not less than 10 6 CFU / m 2 .

2. The method for antibacterial and deodorizing in a livestock and poultry farm according to claim 1, wherein In the slightly acidic electrolyzed water I, the ORP is 700 - 900 mv and the pH is 5.0 - 7.

0.

3. A method for inhibiting bacteria and removing odor in a livestock and poultry farm according to claim 1, characterized in that, In the slightly acidic electrolyzed water II, the ORP is 600 - 700 mv and the pH is 5.0 - 7.

0.

4. A method for inhibiting bacteria and deodorizing in a livestock and poultry farm according to claim 1, characterized in that, The bacterial liquid concentration of the Bacillus subtilis is 10 7 CFU / mL or more.

5. A method for inhibiting bacteria and deodorizing in a livestock and poultry farm according to claim 4, characterized in that, The volume of the bacterial liquid of Bacillus subtilis is 8 - 10 mL.

6. The method for inhibiting bacteria and removing odor in a livestock and poultry farm according to claim 1, wherein It includes the following steps: Using the slightly acidic electrolyzed water I for the first disinfection; After the first disinfection, wait for air drying and then spray the bacterial liquid of Bacillus subtilis; After spraying, use the slightly acidic electrolyzed water II for the second disinfection.

7. A method for inhibiting bacteria and removing odor in livestock and poultry farms according to claim 5, characterized in that, The time for the first disinfection is 12 - 20 min.

8. A method for inhibiting bacteria and deodorizing in a livestock and poultry farm according to claim 7, characterized in that, The bacterial liquid of Bacillus subtilis is sprayed 2 times a day for 3 days.

9. A method for inhibiting bacteria and deodorizing in a livestock and poultry farm according to claim 5, characterized in that, The second disinfection is carried out once a day for 3 days.

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