Pretreatment device for grain mycotoxin detection

Abstract

The invention discloses a pretreatment device for grain mycotoxin detection in the technical field of mycotoxin detection, and the pretreatment device comprises a base, a grinding groove assembly and a first liquid cooling assembly are arranged in the base, a supporting rod is fixedly connected to the base, a grinding head assembly is hinged to the supporting rod, and a second liquid cooling assembly and a liquid supply assembly are arranged in the grinding head assembly. A controller, an extraction liquid storage tank and a cooling liquid storage tank are fixedly connected to the side wall of the base, and the grinding groove assembly, the first liquid cooling assembly, the grinding head assembly, the second liquid cooling assembly and the liquid supply assembly are all in signal connection with the controller. The device is novel and effective, a low-temperature-resistant extracting agent is adaptively supplied while the to-be-detected sample is ground and crushed at a low temperature by the grinding head, the grinding head is rinsed to improve the grinding efficiency, and meanwhile, the extraction effect of the extracting agent on the to-be-detected sample mycotoxin is enhanced by virtue of pressure and shearing force during grinding, so that the accuracy of a subsequent detection result is ensured; the detection work efficiency is improved, and the detection time and difficulty are reduced.

Description

Technical Field

[0001] The present invention belongs to the technical field of mycotoxin detection, and specifically relates to a pretreatment device for detecting mycotoxins in grains. Background Art

[0002] During the processes of planting, harvesting, storing, and processing grains, they are vulnerable to mold contamination and produce various mycotoxins, such as aflatoxin, ochratoxin, zearalenone, deoxynivalenol (vomitoxin), etc. These toxins have strong toxicity, carcinogenicity, teratogenicity, and immunosuppressive properties, seriously threatening the health of humans and animals. Therefore, the detection of mycotoxins in grains has become a necessary means to ensure food safety, reduce economic losses, and protect the health of consumers.

[0003] The traditional pretreatment for detecting mycotoxins in grains is to first crush the grain samples in a low-temperature crusher with a base, and then use an organic solution to dissolve and extract the possible mycotoxins in the grain samples for detection. However, since there is no liquid involved in the low-temperature crushing process to conduct the heat generated by shear and friction, the functions of low temperature to prevent mycotoxin degradation and crushing are both limited to a certain extent. And the thorough low-temperature crushing of these grain samples is an important guarantee for subsequent detection accuracy. Even after sufficient low-temperature crushing, the process of adding an organic solution for extraction is prone to consequences such as uneven stirring and insufficient extraction. Not to mention the tediousness of the two steps of crushing and extraction, which is not conducive to the efficient multi-batch detection of mycotoxins in grains.

[0004] In the patent document with the publication number CN118408801B, a pretreatment device that uniformly sprays an extractant by means of a lead screw nut has been proposed. However, even though its spraying pattern is relatively uniform, it cannot adaptively spray the extractant according to the cumulative position of the grain samples to be uniformly mixed with the grain samples to enhance the extraction effect. Moreover, the heat generated by shear and friction caused by its stirring-type crushing structure is difficult to diffuse from the sample to the outer wall of the container for heat dissipation and cooling, easily resulting in false-negative detection results of mycotoxin degradation.

[0005] Therefore, it is necessary to propose a pretreatment device for detecting mycotoxins in grains that can uniformly and adaptively add an extraction solvent while crushing the grain samples, reduce the adhesion of sample fines to the crushing structure to enhance the crushing effect, and can uniformly stir the grain samples and use the shear force formed by the crushing structure to fully dissolve the mycotoxins in the extractant, ensuring the low-temperature cooling effect to prevent mycotoxin degradation. Summary of the Invention

[0006] To solve the above problems, the object of the present invention is to provide a pretreatment device for detecting mycotoxins in grains. By adaptively supplying a cryogenic-resistant extractant while the grinding head grinds and crushes the sample to be tested at low temperature, it not only rinses the grinding head to improve the grinding efficiency, but also enhances the extraction effect of the extractant on the mycotoxins in the sample to be tested by means of the pressure and shear force during grinding, thus ensuring the accuracy of subsequent detection results, improving the detection work efficiency, and reducing the detection time and difficulty.

[0007] To achieve the above object, the technical solution of the present invention is as follows: A pretreatment device for detecting mycotoxins in grains, including a base, a grinding groove assembly and a first liquid cooling assembly are arranged inside the base, a support rod is fixedly connected to the base, a grinding head assembly is hinged to the support rod, a second liquid cooling assembly and a liquid supply assembly are arranged inside the grinding head assembly, a controller, an extraction liquid storage tank and a cooling liquid storage tank are fixedly connected to the side wall of the base, and the grinding groove assembly, the first liquid cooling assembly, the grinding head assembly, the second liquid cooling assembly and the liquid supply assembly are all connected to the controller in signal connection; The grinding groove assembly is used to provide torque for the pretreatment grinding of grain samples through a planetary gear structure; the first liquid cooling assembly is used to keep the grinding groove assembly at -20°C - 0°C during the pretreatment grinding process by using the liquid cooling principle to avoid the degradation of mycotoxins; the grinding head assembly is used to cooperate with the rotation of the grinding groove assembly for pretreatment grinding; the second liquid cooling assembly is used to keep the grinding head assembly at -20°C - 0°C by using the liquid cooling principle; the liquid supply assembly is used to differentially supply the extractant to rinse the grinding head assembly and homogenize the ground powder during the pretreatment grinding process according to the change in the pressure direction received by the grinding head assembly.

[0008] The principle of the basic solution is: The pretreatment device is composed of multiple components, and each component works together under the signal control of the controller. The grinding groove assembly provides torque for grinding the grain sample through a planetary gear structure, so that the grain sample is ground and crushed in the grinding groove; the first liquid cooling assembly and the second liquid cooling assembly respectively perform liquid cooling on the grinding groove assembly and the grinding head assembly to keep them in a low temperature state to prevent the degradation of mycotoxins during grinding; the grinding head assembly cooperates with the rotation of the grinding groove assembly for grinding; the liquid supply assembly differentially supplies the extractant according to the change in the pressure direction received by the grinding head assembly. On the one hand, it rinses the grinding head assembly to improve the grinding efficiency, on the other hand, it homogenizes the ground powder, and at the same time enhances the extraction effect of the extractant on the mycotoxins in the sample to be tested by means of the pressure and shear force during grinding.

[0009] During the grinding process, the grain sample exerts different pressure directions on the grinding head assembly. The liquid supply assembly can sense the changes in the pressure direction and supply the extraction agent accordingly. When the grinding head assembly is under greater pressure in a certain direction, the liquid supply assembly will increase the supply of the extraction agent near that direction, enabling the extraction agent to better rinse the grinding head assembly, reducing the adhesion of sample debris on the grinding head, and ensuring that the extraction agent can evenly contact the grain sample to promote the full dissolution of mycotoxins.

[0010] The beneficial effects of the basic solution are as follows: 1. By grinding and pulverizing the grain sample in a low-temperature environment and using the pressure and shear force during grinding to enhance the extraction effect of the extraction agent on the mycotoxins in the sample to be tested, the mycotoxins in the grain sample can be retained to the greatest extent, avoiding their degradation or loss during the pretreatment process, thereby ensuring the accuracy of subsequent test results.

[0011] 2. The liquid supply assembly can supply the extraction agent differentially according to the changes in the pressure direction received by the grinding head assembly. It not only rinses the grinding head to improve the grinding efficiency, reduces the adhesion of sample debris on the pulverizing structure, enhances the pulverizing effect, but also makes the pulverized material powder uniform, shortens the pretreatment time and steps of the grain sample, and improves the working efficiency of the entire detection process.

[0012] 3. This device integrates multiple functions such as grinding, cooling, and liquid supply, realizing the integrated operation of the pretreatment for the detection of grain mycotoxins, reducing the equipment and steps required in the detection process, lowering the complexity and difficulty of the detection, and further shortening the detection time.

[0013] 4. The device adopts a modular design, with clear division of labor and reasonable structure among components. Through the controller to control the signals of each component, the automatic operation of the device is realized, reducing the errors and labor intensity of manual operation, and improving the stability and reliability of the device.

[0014] Furthermore, the grinding groove assembly includes a grinding motor, the grinding motor is fixedly connected to the base and the output shaft is vertically upward, a driving gear is coaxially and fixedly connected to the output shaft of the grinding motor, at least 3 equally spaced and circumferentially arranged grinding grooves are rotatably connected to the top wall of the base, and a driven gear meshing with the driving gear is fixedly connected to the outer circumference of each grinding groove. The grinding motor is signal-connected to the controller.

[0015] The beneficial effects of the basic solution are as follows: 1. By meshing the driving gear on the output shaft of the grinding motor with the driven gears on the outer peripheries of multiple surrounding grinding grooves, a transmission structure similar to a planetary gear is formed. This structure can convert the single rotational motion of the grinding motor into the synchronous rotational motion of multiple grinding grooves, and the rotational directions and speeds of the respective grinding grooves can be precisely controlled according to the gear transmission ratio, providing a stable and powerful torque for the pretreatment grinding of grain samples, ensuring that the grain samples can be fully ground and crushed, and improving the grinding efficiency and quality. The simultaneous rotation of multiple grinding grooves increases the contact area and contact frequency between the grain samples and the grinding structure, enabling the grain samples to be uniformly ground into fine powders in a shorter time, further improving the grinding effect, and providing more favorable conditions for the subsequent extraction of mycotoxins.

[0016] 2. This design allows the number of grinding grooves to be increased or decreased according to actual needs. When a large number of grain samples need to be processed, the number of grinding grooves can be increased to improve the overall grinding processing capacity; while in the case of processing a small number of samples or having requirements for the device space, the number of grinding grooves can be decreased to make the device more compact. This scalability enables the pretreatment device to adapt to grain mycotoxin detection tasks of different scales and requirements. The grinding grooves are rotatably connected to the top wall of the base, and each grinding groove is relatively independent. When a certain grinding groove is worn or fails, it can be maintained or replaced individually without affecting the normal operation of other grinding grooves, reducing the maintenance cost and downtime of the device, and improving the reliability and service life of the device.

[0017] Furthermore, the first liquid cooling component includes a first cold liquid pump, the first cold liquid pump is communicated with the cooling liquid storage tank, the output end of the first cold liquid pump is communicated with a number of groove liquid cooling pipes corresponding to the number of grinding grooves, first liquid cooling grooves and first waste liquid grooves are respectively opened inside the walls of the grinding grooves, the first liquid cooling grooves are all laid in a spiral funnel shape and the ends are all communicated with the first waste liquid grooves, the first waste liquid grooves all extend from the top to the bottom of the grinding grooves and are fixedly communicated with annular sliding members, the groove liquid cooling pipes are respectively rotationally communicated with the ends of the first liquid cooling grooves located at the centers of the bottoms of the grinding grooves, annular sliding grooves are respectively rotationally connected to the outer peripheries of the groove liquid cooling pipes, the annular sliding grooves are all slidably mated with the corresponding annular sliding members to communicate the first waste liquid grooves with the annular sliding grooves, first recovery pipes are all communicated with the bottom walls of the annular sliding grooves, the first recovery pipes are all communicated with the cooling liquid storage tank, and the first cold liquid pump is signal-connected to the controller.

[0018] The beneficial effects of the basic solution are as follows: 1. The first liquid cooling tank is laid in a spiral funnel shape inside the wall of the grinding tank. This design greatly increases the contact area between the coolant and the wall of the grinding tank, enabling the coolant to absorb the heat generated during the grinding process more fully and evenly, thereby more effectively maintaining the grinding tank at a low temperature. The spiral structure can also guide the coolant to flow along a specific path, extending the residence time of the coolant in the grinding tank, improving the cooling efficiency, and ensuring that the grain sample will not cause mycotoxin degradation due to high temperature during the grinding process. Each grinding tank corresponds to an independent tank liquid cooling pipe, the first liquid cooling tank, and the first waste liquid tank, forming an independent cooling circulation system. This design avoids the interference of the coolant between different grinding tanks and can precisely control the flow rate and temperature of the coolant according to the actual working conditions of each grinding tank (such as grinding intensity, grain sample quantity, etc.), realizing the precise control of the temperature of each grinding tank and further improving the effect of low-temperature maintenance.

[0019] 2. The tank liquid cooling pipe is rotationally connected to the first liquid cooling tank opening at the center of the bottom of the grinding tank. At the same time, a ring-shaped sliding groove is rotationally connected to the outer periphery of the tank liquid cooling pipe, and the ring-shaped sliding groove is slidably matched with the ring-shaped sliding member of the first waste liquid tank extending from the grinding tank opening to the bottom. This design enables the input and output channels of the coolant to always remain unobstructed during the rotation of the grinding tank and will not be hindered by the rotation of the grinding tank, ensuring the smooth circulation of the coolant. After the first waste liquid tank collects the used coolant, the waste liquid is transported to the ring-shaped sliding groove through the sliding fit of the ring-shaped sliding member and the ring-shaped sliding groove, and then flows back to the cooling liquid storage tank through the first recovery pipe. This waste liquid recovery system not only realizes the recycling of the coolant, reduces costs, but also avoids the pollution of the environment by the waste liquid, while ensuring the stable operation of the cooling system and reducing the temperature fluctuation problem of the grinding tank caused by coolant leakage or insufficient supply.

[0020] Furthermore, the grinding head assembly includes a top cover, the top cover is hinged to the support rod, column-shaped grooves corresponding to the number of grinding tanks are opened on the bottom wall of the top cover, through holes are opened at the centers of the bottom walls of the column-shaped grooves, grinding springs are fixedly connected to the bottom walls of the column-shaped grooves, the bottom ends of the grinding springs are fixedly connected to grinding heads, and stabilizing rods are fixedly connected to the top walls of the grinding heads. The stabilizing rods respectively pass through the corresponding grinding springs and through holes and extend into the column-shaped grooves.

[0021] The beneficial effects of the basic scheme are as follows: 1. During the grinding process, the hardness, particle size and initial state of different grain samples are different, resulting in different pressures required for grinding. The grinding spring can automatically expand and contract according to the reaction force of the grain sample on the grinding head, thereby adjusting the distance and pressure between the grinding head and the grinding groove. When encountering a harder grain sample, the grinding spring is compressed, and the pressure between the grinding head and the grinding groove increases to enhance the grinding force; when the grain sample is gradually crushed and the hardness decreases, the grinding spring stretches, and the distance between the grinding head and the grinding groove increases to avoid over-grinding. This adaptive adjustment ensures that the grain sample can be crushed efficiently and evenly. The stabilizing rod passes through the grinding spring and the through hole and extends into the columnar groove, providing a stable guide for the movement of the grinding head. During the grinding process, the grinding head will be subjected to forces from all directions of the grain sample and the elastic force of the grinding spring. The presence of the stabilizing rod can prevent the grinding head from excessive displacement or shaking during movement, improve the stability and accuracy of the grinding, and ensure the uniformity of the crushing of the grain sample.

[0022] 2. The hinged design of the top cover and the support rod makes the grinding head assembly more flexible. When placing and removing grain samples, you only need to lift the top cover to easily put the grain sample into the grinding tank or take out the ground powder. The operation is simple and fast, which greatly improves work efficiency. At the same time, this design also facilitates the cleaning and maintenance of the grinding head assembly. When grinding grain samples of different types or batches, the grinding head and grinding tank can be cleaned in time to avoid sample residues affecting subsequent test results.

[0023] Furthermore, the second liquid cooling component includes a second cold liquid pump, which is connected to the cooling liquid storage tank. The output end of the second cold liquid pump is connected to a head liquid cooling pipe corresponding to the number of grinding heads. A second liquid cooling groove is opened inside the grinding head. The second liquid cooling groove vertically passes through the corresponding stabilizing rod and the grinding head and is laid in a spiral funnel shape inside the bottom wall of the grinding head. The end of the second liquid cooling groove is connected to a second waste liquid groove, and the second waste liquid groove passes upward through the grinding head and the stabilizing rod. The head liquid cooling pipes are respectively connected to the top of the corresponding second liquid cooling groove, and the top of the second waste liquid groove is connected to a second recovery pipe, which is connected to the cooling liquid storage tank. The second cold liquid pump is connected to the controller signal.

[0024] The beneficial effects of the basic solution are as follows: 1. The second liquid cooling component is specifically designed to cool the grinding head. Since the grinding head is in direct contact with the grain sample during the grinding process, a large amount of frictional heat is generated, which easily causes the temperature of the grinding head to rise. The second liquid cooling tank vertically passes through the stabilizing rod and the grinding head and is laid in a spiral funnel shape inside the bottom wall of the grinding head. This design enables the coolant to directly act on the key heat-generating parts of the grinding head, such as the bottom wall in contact with the grain sample, achieving highly efficient local targeted cooling, effectively reducing the temperature of the grinding head, preventing the degradation of mycotoxins in the grain sample due to high temperature, and ensuring the accuracy of subsequent test results. The spiral funnel-shaped second liquid cooling tank increases the flow path and contact area of the coolant inside the grinding head, enabling the coolant to more evenly absorb the heat of the grinding head and avoiding the occurrence of local overheating. At the same time, the coolant can continuously carry away heat during the flow process, maintaining the uniformity and stability of the temperature of the grinding head, ensuring that the grinding process is carried out at an appropriate temperature, and improving the grinding quality and efficiency.

[0025] 2. Effective cooling can reduce heat damage to the grinding head caused by high temperature, such as thermal deformation, increased wear, etc., extend the service life of the grinding head, and reduce the maintenance cost of the device. A stable cooling system can ensure that the grinding head always maintains a good working state during long-term and high-intensity grinding work, avoid system failures caused by excessive temperature, improve the reliability and stability of the entire pretreatment device, and provide reliable sample pretreatment guarantee for the detection of grain mycotoxins.

[0026] Furthermore, the liquid supply component includes a liquid supply pump. The liquid supply pump is connected to the extraction liquid storage tank. The output end of the liquid supply pump is connected to a liquid supply pipe. The liquid supply pipe is connected to at least 4 liquid distribution pipes. Liquid distribution valves are provided on each liquid distribution pipe. At least 4 piston cylinders are fixedly connected to the inner top wall of the columnar tank. The piston cylinders in the same columnar tank are distributed around the corresponding stabilizing rods. A piston block is slidably fitted in each piston cylinder. A push rod is fixedly connected to one side of each piston block close to the corresponding stabilizing rod. A push arc block is fixedly connected to the end of the push rod. The push arc blocks in the same columnar tank are distributed on the outer periphery of the corresponding stabilizing rods. A return spring is fixedly connected to each piston block. The other end of the return spring is fixedly connected to the inner wall of the piston cylinder. The return spring surrounds the corresponding push rod. The liquid distribution pipes are respectively connected to the corresponding piston cylinders. Each piston cylinder is connected to a liquid spraying pipe. The liquid spraying pipe extends downward to the bottom wall of the top cover around the grinding head and is connected. The liquid spraying pipe orifices all face the surface of the grinding head and are all provided with silicone valves. The liquid supply pump and the liquid distribution valves are both signal-connected to the controller.

[0027] The beneficial effects of the basic solution are as follows: 1. A liquid separation valve is provided on the liquid separation pipe, enabling independent control of the liquid supply volume for each piston cylinder. When different grinding heads grind different grain samples, the directions and magnitudes of the pressures they receive will vary, and the demand for the extractant also differs. Through precise control of the liquid separation valve by the controller, an appropriate amount of extractant can be supplied to each piston cylinder according to actual needs, avoiding the problem of uneven extractant supply caused by unified liquid supply in the traditional liquid supply method, ensuring that each grinding head can obtain the right amount of extractant, and improving the accuracy and efficiency of mycotoxin extraction.

[0028] 2. The design of the piston block, push rod, and push arc block can respond to changes in the pressure direction received by the grinding head. When the grinding head receives pressures in different directions during the grinding process, the push arc block will be squeezed or released, driving the push rod and piston block to slide within the piston cylinder. This dynamic mechanical structure enables the liquid supply assembly to sense the grinding state in real time and automatically adjust the liquid supply volume and timing according to the pressure change, achieving adaptive and differential liquid supply, and further improving the accuracy of liquid supply.

[0029] 3. Multiple piston cylinders and liquid spraying pipes are provided within the same columnar groove, and the liquid spraying pipes are distributed around the grinding head with the liquid spraying pipe orifices facing the surface of the grinding head. This design allows the extractant to be evenly sprayed onto the grinding head from multiple directions, achieving full coverage of the grinding head, avoiding the situation of excessive or insufficient spraying of the extractant in certain areas, ensuring that the ground material powder can uniformly contact the extractant, and improving the uniformity and consistency of mycotoxin extraction.

[0030] Furthermore, both the first liquid cooling pipe and the second recovery pipe are made of flexible materials.

[0031] The beneficial effects of the basic solution are as follows: 1. During the operation of the grinding head assembly, the stabilizing rod will move up and down and swing to a certain extent along with the grinding operation. The first liquid cooling pipe and the second recovery pipe made of flexible materials can freely bend and stretch along with the movement of the grinding head, without hindering the normal movement of the grinding head, ensuring the smooth progress of the grinding process, and improving the grinding efficiency and stability. The first liquid cooling pipe and the second recovery pipe made of flexible materials can effectively avoid the occurrence of pipeline wear, deformation, or even damage, reduce mechanical wear, and extend the service life of the pipeline.

[0032] 2. During the grinding process, the grinding head is subjected to relatively large forces and vibrations. When rigid pipes are subjected to these forces and vibrations, they are prone to rupture, resulting in coolant leakage or the inability to properly recover waste liquid. Flexible materials have good flexibility and impact resistance, can withstand the forces and vibrations generated by the movement of the grinding head, effectively prevent pipe rupture, and ensure the reliability of the cooling and recovery system. The first liquid cooling pipe and the second recovery pipe made of flexible materials can maintain good sealing performance during the bending and stretching processes, preventing coolant and waste liquid leakage. This is crucial for maintaining the normal operation of the entire cooling and recovery system, avoiding problems such as a decline in cooling effect and environmental pollution caused by leakage.

[0033] 3. When rigid pipes are subjected to the vibrations generated by the movement of the grinding head, they will directly transmit the vibrations to the entire cooling and recovery system, resulting in relatively large noise in the system. The first liquid cooling pipe and the second recovery pipe made of flexible materials have a certain shock-absorbing effect, can absorb and buffer vibrations, reduce the transmission of vibrations to the system, thereby reducing the noise during system operation and improving the working environment.

[0034] Furthermore, spiral grooves are neatly arranged on the bottom surface of the grinding head, and a grinding layer is laid on the inner wall of the grinding groove.

[0035] The beneficial effects of the basic solution are as follows: 1. The spiral grooves neatly arranged on the bottom surface of the grinding head can, during the grinding process, like the threads on a drill bit, have a combined effect of cutting and extruding the grain sample. The spiral grooves can gradually guide and crush the grain sample, increasing the contact area and the acting force between the grinding head and the grain sample, enabling the grain sample to be broken into powder more quickly, greatly improving the grinding efficiency, and shortening the pretreatment time.

[0036] 2. The rough grinding layer laid on the inner wall of the grinding groove cooperates with the spiral grooves on the bottom of the grinding head. When the grinding head rotates in the grinding groove, the rough grinding layer will have a frictional and grinding effect on the grain sample, further refining the grain sample. The two work together to quickly grind the grain sample into uniform powder, providing better sample conditions for subsequent mycotoxin extraction.

[0037] 3. The neat arrangement of the spiral grooves enables the acting force on the grain sample by the grinding head to be more evenly distributed during the grinding process. It can evenly transmit the grinding force to all parts of the grain sample, avoiding the occurrence of local over-grinding or under-grinding, thus ensuring that the particle size of the ground grain sample powder is uniform.

[0038] Furthermore, a sealing layer matching the top wall of the base is laid on the bottom wall of the top cover.

[0039] The beneficial effects of the basic solution are as follows: 1. During the pretreatment process of grain mycotoxin detection, an extractant is used to soak and extract the ground grain powder, and waste liquid is generated at the same time. The sealing layer laid on the bottom wall of the top cover fits tightly with the top wall of the base, which can form a closed space, effectively preventing the extractant and waste liquid from leaking out through the gap between the grinding groove and the top cover, avoiding environmental pollution, and at the same time ensuring the stability and accuracy of the extraction process. The presence of the sealing layer can also prevent external dust, impurities, etc. from entering the grinding groove, ensuring the purity of the extractant and the originality of the grain sample, thereby improving the reliability of the mycotoxin detection results.

[0040] 2. The first liquid cooling component and the second liquid cooling component respectively provide cooling for the grinding groove and the grinding head to maintain a low-temperature environment, which is beneficial to the extraction of mycotoxins. The sealing layer can reduce the heat exchange between the grinding groove and the external environment, slow down the heat dissipation rate, make the temperature in the grinding groove more stable, ensure that the extraction process is carried out at an appropriate temperature, and improve the extraction efficiency of mycotoxins. The stable temperature environment can avoid the influence of temperature fluctuations on the mycotoxins in the grain sample, ensuring the accuracy and consistency of the detection results.

[0041] Furthermore, refrigerators are fixedly connected to both the extraction liquid storage tank and the cooling liquid storage tank for precooling the extraction solvent and cooling the cooling liquid. A locking member for cooperating with the base is provided on one side of the top cover, and the refrigerators are both signal-connected to the controller.

[0042] The beneficial effects of the basic solution are as follows: 1. The refrigerator fixedly connected to the extraction liquid storage tank can precool the extraction solvent. During the pretreatment process of grain mycotoxin detection, a low-temperature environment helps to maintain the stability and activity of mycotoxins and can improve the extraction efficiency at the same time. The precooled extraction solvent can penetrate into the grain sample more quickly, dissolve the mycotoxins more fully, and thus obtain more accurate detection results. The refrigerator on the cooling liquid storage tank can continuously provide a low-temperature environment for the cooling liquid. During the grinding process, the first liquid cooling component and the second liquid cooling component rely on the low-temperature cooling liquid to cool the grinding groove and the grinding head, preventing the mycotoxins in the grain sample from denaturing or decomposing due to heat generated by friction. The presence of the refrigerator ensures that the cooling liquid is always at an appropriate low temperature, providing a stable temperature guarantee for the entire grinding and extraction process. The automatic temperature control function of the refrigerator enables the operator to set the target temperature without manually adjusting the temperature frequently. The simple operation method of the locking member also reduces the operation steps, improving the operation convenience and efficiency of the entire pretreatment process.

[0043] 2. The locking piece on one side of the top cover that matches the base can firmly fix the top cover to the base during the grinding and extraction process. This not only ensures the stability of the device during operation, prevents liquid leakage and equipment failure caused by loose top cover, but also facilitates the operator to open and close the equipment. When it is necessary to add grain samples or perform equipment maintenance, the operator can easily unlock the locking piece and open the top cover for operation. BRIEF DESCRIPTION OF THE DRAWINGS

[0044] Figure 1 This is an axonometric diagram of a pre-treatment device for detecting mycotoxins in grains according to an embodiment of the present invention.

[0045] Figure 2 It is a top view of a pre-treatment device for detecting mycotoxins in grains in an embodiment of the present invention.

[0046] Figure 3 It is a side cross-sectional view of a pre-treatment device for detecting mycotoxins in grains in an embodiment of the present invention.

[0047] Figure 4 For the embodiment of the present invention Figure 3 Enlarged view of part A.

[0048] The reference numerals in the drawings of the specification include: 1, base; 2, sealing layer; 3, locking member; 4, top cover; 5, support rod; 6, cooling liquid storage tank; 7, refrigerator; 8, second cold liquid pump; 9, first cold liquid pump; 10, second recovery pipe; 11, head liquid cooling pipe; 12, first recovery pipe; 13, tank liquid cooling pipe; 14, controller; 15, liquid supply pipe; 16, liquid supply pump; 17, extraction liquid storage tank; 18, grinding motor; 19, annular slide groove; 20, annular Sliding part; 21, first waste liquid tank; 22, first liquid cooling tank; 23, grinding tank; 24, second liquid cooling tank; 25, driven gear; 26, grinding head; 27, spiral pattern; 28, liquid dispensing pipe; 29, liquid dispensing valve; 30, piston cylinder; 31, liquid spraying pipe; 32, piston block; 33, return spring; 34, push rod; 35, push arc block; 36, through hole; 37, grinding spring; 38, stabilizing rod; 39, second waste liquid tank; 40, driving gear. DETAILED DESCRIPTION

[0049] The following is further described in detail through specific implementation methods: Example 1

[0050] Basically as attached Figure 1 , Figure 2 , Figure 3 and Figure 4As shown: A pretreatment device for detecting mycotoxins in grains, including a base 1. Inside the base 1, a grinding groove assembly and a first liquid cooling assembly are installed. A support rod 5 is welded to the base 1, and a grinding head assembly is hinged on the support rod 5. Inside the grinding head assembly, a second liquid cooling assembly and a liquid supply assembly are installed. A controller 14, an extraction liquid storage tank 17, and a cooling liquid storage tank 6 are welded to the side wall of the base 1. The grinding groove assembly, the first liquid cooling assembly, the grinding head assembly, the second liquid cooling assembly, and the liquid supply assembly are all connected to the controller 14 by signal.

[0051] A sealing layer 2 matching the top wall of the base 1 is laid on the bottom wall of the top cover 4. Refrigerators 7 are welded and connected to both the extraction liquid storage tank 17 and the cooling liquid storage tank 6 for precooling the extraction solvent and cooling the cooling liquid. A locking member 3 matching the base 1 is installed on one side of the top cover 4. The refrigerators 7 are all connected to the controller 14 by signal.

[0052] The grinding groove assembly is used to provide torque for the pretreatment grinding of grain samples by rotating through a planetary gear structure. The grinding groove assembly includes a grinding motor 18. The grinding motor 18 is welded to the base 1 and its output shaft is vertically upward. A driving gear 40 is coaxially welded to the output shaft of the grinding motor 18. At least 3 equally spaced and circumferentially arranged grinding grooves 23 are rotatably connected to the top wall of the base 1. Driven gears 25 meshing with the driving gear 40 are welded to the outer circumference of each grinding groove 23. The grinding motor 18 is connected to the controller 14 by signal.

[0053] The first liquid cooling assembly is used to keep the grinding groove assembly at -20°C - 0°C during the pretreatment grinding process by using the liquid cooling principle to avoid the degradation of mycotoxins. The first liquid cooling assembly includes a first cold liquid pump 9. The first cold liquid pump 9 is connected to the cooling liquid storage tank 6. The output end of the first cold liquid pump 9 is connected to a groove liquid cooling pipe 13 corresponding to the number of grinding grooves 23. First liquid cooling grooves 22 and first waste liquid grooves 21 are respectively opened inside the walls of the grinding grooves 23. The first liquid cooling grooves 22 are all laid in a spiral funnel shape and their ends are all connected to the first waste liquid grooves 21. The first waste liquid grooves 21 all extend from the top of the grinding grooves 23 to the bottom of the grinding grooves 23 and are welded and connected to an annular sliding member 20. The groove liquid cooling pipes 13 are respectively rotatably connected to the ends of the first liquid cooling grooves 22 located at the center of the bottom of the grinding grooves 23. Annular sliding grooves 19 are rotatably connected to the outer circumference of the groove liquid cooling pipes 13. The annular sliding grooves 19 are all slidably matched with the corresponding annular sliding members 20 to connect the first waste liquid grooves 21 with the annular sliding grooves 19. First recovery pipes 12 are connected to the bottom walls of the annular sliding grooves 19. The first recovery pipes 12 are all connected to the cooling liquid storage tank 6. The first cold liquid pump 9 is connected to the controller 14 by signal.

[0054] The grinding head assembly is used to cooperate with the rotation of the grinding groove assembly for pre-processing grinding. The grinding head assembly includes a top cover 4, which is hinged to the support rod 5. The bottom wall of the top cover 4 is provided with columnar grooves corresponding to the number of grinding grooves 23. A through hole 36 is provided in the center of the bottom wall of the columnar groove. Grinding springs 37 are welded on the bottom walls of the columnar grooves. Grinding heads 26 are welded to the bottom ends of the grinding springs 37. Stabilizing rods 38 are welded to the top walls of the grinding heads 26. The stabilizing rods 38 pass through the corresponding grinding springs 37 and through holes 36 respectively and extend to the inside of the columnar grooves. The bottom surface of the grinding head 26 is provided with neatly arranged spiral patterns 27, and the inner walls of the grinding grooves 23 are paved with grinding layers.

[0055] The second liquid cooling component is used to keep the grinding head at -20℃-0℃ using the liquid cooling principle. The second liquid cooling component includes a second cold liquid pump 8, which is connected to the cooling liquid storage tank 6. The output end of the second cold liquid pump 8 is connected to a head liquid cooling pipe 11 corresponding to the number of grinding heads 26. A second liquid cooling groove 24 is opened inside the grinding head 26. The second liquid cooling groove 24 vertically passes through the corresponding stabilizing rod 38 and the grinding head 26 and is laid in a spiral funnel shape inside the bottom wall of the grinding head 26. The end of the second liquid cooling groove 24 is connected to a second waste liquid groove 39, and the second waste liquid groove 39 passes upward through the grinding head 26 and the stabilizing rod 38. The head liquid cooling pipe 11 is respectively connected to the top of the corresponding second liquid cooling groove 24, and the top of the second waste liquid groove 39 is connected to a second recovery pipe 10. The second recovery pipe 10 is connected to the cooling liquid storage tank 6. The second cold liquid pump 8 is connected to the controller 14 signal. The head liquid cooling pipe 11 and the second recovery pipe 10 are both made of flexible material.

[0056] The liquid supply component is used to differentially supply the extractant to rinse the grinding head component and homogenize the ground material powder according to the change in the direction of the pressure applied to the grinding head component during the pre-grinding process.

[0057] The specific implementation process is as follows: the testing staff puts the grain sample into the upper grinding groove 23 of the base 1, flexibly selects the use of the grinding groove 23 according to the number of sample types and sample quantities, closes the top cover 4 so that the sealing layer 2 is completely in contact with the top wall of the base 1, and locks the locking member 3 to ensure that the device is airtight. The controller 14 then starts the refrigerator 7 to pre-cool the extractant and coolant in the extraction liquid storage tank 17 and the cooling liquid storage tank 6, respectively, to create a low-temperature environment for pre-treatment. The mycotoxin extractant of the grain sample is generally methanol or acetonitrile, both of which have a melting point below -20°C and will not be frozen by the protective low temperature of the pre-treatment device.

[0058] After reaching the protective low temperature (such as -20°C), the grinding motor 18 inside the base 1 starts to run, and its output shaft drives the driving gear 40 to rotate, and drives the surrounding driven gears 25 to rotate synchronously through the planetary gear transmission mechanism, so that each grinding groove 23 revolves at a preset differential ratio. This movement mode causes the sample to roll in multiple directions in the grinding groove 23, and cooperates with the rough grinding layer on the inner wall of the grinding groove 23 to achieve efficient crushing and mixing. In the columnar groove, the grinding spring 37 pushes the bottom welded grinding head 26 to contact the inner wall of the rotating grinding groove 23. The stabilizing rod 38 at the top of the grinding head 26 is in the through hole 36, which not only limits the deviation of the grinding head 26, but also leaves sufficient deviation space to trigger the adaptive spraying of the liquid supply component. Under the action of friction, the spiral pattern 27 at the bottom of the grinding head 26 pushes the sample powder evenly, and the sample powder falls under the influence of gravity, so that it is repeatedly and evenly crushed, and at the same time, it cooperates with the rough layer on the inner wall of the grinding groove 23 to enhance the crushing effect.

[0059] The first liquid cooling component is started synchronously, and the first cold liquid pump 9 pumps the low-temperature coolant in the cooling liquid storage tank 6 into the tank liquid cooling pipe 13. The coolant flows along the spiral funnel-shaped first liquid cooling tank 22 inside the wall of the grinding tank 23. The coolant first reaches the bottom of the grinding tank 23 where a large number of grain samples are gathered to ensure the low temperature effect. After fully absorbing the heat generated by grinding, it passes through the dynamic channel formed by the annular slide 19 and the annular slide 20, and flows back to the cooling liquid storage tank 6 through the first recovery pipe 12, forming a closed-loop cooling path, which effectively prevents fungal toxins from being degraded due to high temperature of vermicelli. The second liquid cooling assembly acts independently on the grinding head assembly. The second cold liquid pump 8 delivers the coolant through the flexible head liquid cooling tube 11 and through the stabilizing rod 38 to the second liquid cooling tank 24 inside the grinding head 26. After the coolant flows downward through the vertical channel, it also first reaches the bottom of the grinding head 26 where it contacts a large amount of grain samples. Then it flows in a spiral funnel shape inside the bottom wall of the grinding head 26, and finally returns to the cooling liquid storage tank 6 through the second waste liquid tank 39 and the flexible second recovery tube 10, thereby cooling the grinding head 26 and further ensuring the stability of the toxin molecules. The flexible head liquid cooling tube 11 and the second recovery tube 10 are mainly used to cooperate with the stabilizing rod 38 that may shake and vibrate during the grinding process to avoid pipe rupture and limit the mobility of the stabilizing rod 38.

[0060] like Figure 4As shown, during the grinding process, the liquid supply assembly continuously sprays the extractant on the periphery of the grinding head 26, and the extractant flows downward along the spiral lines 27 on the periphery of the grinding head 26 to wash off the grain sample powder adhered thereto, and at the same time, the crushing process and the extraction process are combined into one, simplifying the pre-treatment process, and enhancing the extraction effect by means of the pressure exerted by the grinding head 26 on the grain sample during the crushing process. If the grain sample in a certain grinding groove 23 is concentrated on one side of the grinding head 26, the activity of the grinding spring 37 will cause the stabilizing rod 38 on the grinding head 26 to be pushed in the same direction, thereby squeezing the liquid supply assembly, so that a larger amount of the extractant relative to the continuously added is squeezed and sprayed to the corresponding position, flushing the concentrated grain sample, ensuring the uniform crushing of the grain sample, promoting the rapid dissolution of the toxin molecules and forming a uniform suspension, and providing high-quality samples for subsequent testing.

[0061] The entire process is carried out in an orderly manner under the control of the controller 14. The various components work together to integrate the crushing and extraction and maintain a low temperature environment throughout the process to ensure the integrity and detection accuracy of the mycotoxins. After the top cover 4 is opened, the collected powder-solvent mixture can be directly introduced into the detection equipment after static filtration, completing an efficient and accurate pre-treatment process. Example 2

[0062] The difference from the above embodiment is that, as shown in the attached Figure 1 , Figure 2 , Figure 3 and Figure 4 As shown: the liquid supply assembly includes a liquid supply pump 16, the liquid supply pump 16 is connected to the extraction liquid storage tank 17, the output end of the liquid supply pump 16 is connected to the liquid supply pipe 15, the liquid supply pipe 15 is connected to at least 4 liquid distribution pipes 28, and the liquid distribution pipes 28 are provided with liquid distribution valves 29. At least 4 piston cylinders 30 are welded to the top wall of the columnar groove. The piston cylinders 30 in the same columnar groove are distributed around the corresponding stabilizing rods 38. The piston cylinders 30 are slidably matched with piston blocks 32. The piston blocks 32 are welded with push rods 34 on the side close to the corresponding stabilizing rods 38, and the end of the push rods 34 are welded with push arc blocks 35. A pushing arc block 35 in a cylindrical groove is distributed on the outer periphery of the corresponding stabilizing rod 38, a return spring 33 is welded on the piston block 32, and the other end of the return spring 33 is welded to the inner wall of the piston cylinder 30, and the return spring 33 surrounds the outer periphery of the corresponding pushing rod 34, and the liquid dispensing pipe 28 is respectively connected to the corresponding piston cylinder 30, and the piston cylinder 30 is connected to the liquid spraying pipe 31, which extends downward to the bottom wall of the top cover 4 around the grinding head 26 and is connected, and the mouth of the liquid spraying pipe 31 faces the surface of the grinding head 26 and is paved with a silicone valve, and the liquid supply pump 16 and the liquid dispensing valve 29 are both connected to the controller 14 signal.

[0063] The specific implementation process is as follows: During the pulverization process, the liquid supply pump 16 of the liquid supply assembly is responsible for stably and continuously pumping the extractant into the corresponding grinding tank 23. The output end of the liquid supply pump 16 is connected to the liquid supply pipe 15, and the liquid supply pipe 15 transports the extractant to each branch, that is, the liquid distribution pipe 28. Each liquid distribution pipe 28 is provided with a precise liquid distribution valve 29. Under the precise control of the controller 14, these valves can dynamically adjust the flow rate of each liquid distribution pipe 28 according to the usage of the grinding tank 23 to ensure that the extractant can be accurately delivered to the required position.

[0064] In each columnar tank, at least 4 piston cylinders 30 are fixedly installed on the top wall, evenly distributed around the corresponding stabilizing rod 38. One side of the piston cylinder 30 is tightly connected to the pushing arc block 35 through the pushing rod 34, and the pushing arc blocks 35 are cleverly distributed on the outer periphery of the stabilizing rod 38, forming a dynamic feedback mechanism. When the grain sample is unevenly distributed during the grinding process, resulting in a greater pressure on one side of the grinding head 26, the stabilizing rod 38 will generate a pressure in the same direction. This pressure change will be quickly transmitted to the pushing arc block 35, which will then push the piston block 32 to move within the piston cylinder 30, compressing the return spring 33. At the same time, it will push the extractant in the piston cylinder 30 into the liquid spraying pipe 31, so that a much larger amount than the continuously added extractant is sprayed out from the orifice of the liquid spraying pipe 31. After spraying, when the uniformity of the grain sample is improved, the pressure on the grinding head 26 is balanced at this time, and the stabilizing rod 38 also maintains its balanced position. The return spring 33 pushes the piston to reset and refill the extractant in the piston cylinder 30, preparing for the next spraying.

[0065] The liquid spraying pipe 31 extends downward to the bottom wall of the top cover 4 around the grinding head 26 and is connected, and the liquid spraying orifices are all oriented towards the surface of the grinding head 26 to ensure that the extractant can directly act on the grinding head 26 and the grain sample around it, effectively flushing to promote the uniform pulverization of the grain sample and dissolve the toxin molecules therein.

[0066] The entire liquid supply process proceeds orderly under the precise control of the controller 14. The stable operation of the liquid supply pump 16, the precise control of the liquid distribution valve 29, the flexible response of the piston cylinder 30, and the directional spraying of the liquid spraying pipe 31 together constitute an efficient and precise adaptive extractant addition system. This design not only simplifies the pretreatment process and improves work efficiency, but more importantly, it can dynamically adjust the dosage of the extractant according to the actual distribution of the grain sample to ensure the rapid dissolution and uniform suspension of the toxin molecules, providing a strong guarantee for the subsequent high-quality detection.

[0067] It should be noted that in this text, relational terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the term "comprising", "including" or any other variant thereof is intended to cover non-exclusive inclusion, so that a process, method, article or device comprising a series of elements not only includes those elements, but also includes other elements not expressly listed, or further includes elements inherent to such process, method, article or device.

[0068] The above are only embodiments of the present invention. Common knowledge such as the specific structures and characteristics in the solutions is not described in detail here. Those of ordinary skill in the art know all the common general technical knowledge in the technical field to which the invention pertains before the filing date or the priority date, are able to know all the existing technologies in this field, and have the ability to apply the conventional experimental means before this date. Those of ordinary skill in the art can, under the inspiration given in this application, combine their own abilities to complete and implement this solution. Some typical well-known structures or well-known methods should not become an obstacle for those of ordinary skill in the art to implement this application. It should be pointed out that for those skilled in the art, without departing from the structure of the present invention, several deformations and improvements can still be made, and these should also be regarded as the protection scope of the present invention, and these will not affect the implementation effect of the present invention and the practicality of the patent. The protection scope required by this application should be based on the content of its claims, and the specific implementation manners described in the specification can be used to interpret the content of the claims.

Claims

1. A pretreatment device for detecting mycotoxins in grains, comprising a base (1), characterized in that: Inside the base (1), there are a grinding groove assembly and a first liquid cooling assembly. A support rod (5) is fixedly connected to the base (1). A grinding head assembly is hinged to the support rod (5). Inside the grinding head assembly, there are a second liquid cooling assembly and a liquid supply assembly. A controller (14), an extraction liquid storage tank (17), and a cooling liquid storage tank (6) are fixedly connected to the side wall of the base (1). The grinding groove assembly, the first liquid cooling assembly, the grinding head assembly, the second liquid cooling assembly, and the liquid supply assembly are all in signal connection with the controller (14); The grinding groove assembly is used to provide torque for the pretreatment grinding of grain samples through the rotation of the planetary gear structure; the first liquid cooling assembly is used to keep the grinding groove assembly at -20°C - 0°C during the pretreatment grinding process by using the liquid cooling principle to avoid the degradation of mycotoxins; the grinding head assembly is used to cooperate with the rotation of the grinding groove assembly for pretreatment grinding; the second liquid cooling assembly is used to keep the grinding head assembly at -20°C - 0°C by using the liquid cooling principle; the liquid supply assembly is used to differentially supply an extraction agent to rinse the grinding head assembly and homogenize the ground powder according to the change in the pressure direction received by the grinding head assembly during the pretreatment grinding process.

2. The pretreatment device for detecting grain mycotoxins according to claim 1, wherein: The grinding groove assembly includes a grinding motor (18). The grinding motor (18) is fixedly connected to the base (1) and its output shaft is vertically upward. A driving gear (40) is coaxially and fixedly connected to the output shaft of the grinding motor (18). At least 3 equally spaced and circumferentially arranged grinding grooves (23) are rotatably connected to the top wall of the base (1). A driven gear (25) meshing with the driving gear (40) is fixedly connected to the outer periphery of each grinding groove (23). The grinding motor (18) is in signal connection with the controller (14).

3. The pretreatment device for detecting grain mycotoxins according to claim 1, wherein: The first liquid cooling assembly includes a first cold liquid pump (9). The first cold liquid pump (9) is communicated with the cooling liquid storage tank (6). The output end of the first cold liquid pump (9) is communicated with a groove liquid cooling pipe (13) corresponding to the number of grinding grooves (23). Inside the wall of each grinding groove (23), a first liquid cooling groove (22) and a first waste liquid groove (21) are opened. The first liquid cooling grooves (22) are all laid in a spiral funnel shape and their ends are all communicated with the first waste liquid groove (21). The first waste liquid grooves (21) all extend from the top of the grinding groove (23) to the bottom of the grinding groove (23) and are fixedly communicated with an annular sliding part (20). The groove liquid cooling pipes (13) are respectively rotationally communicated with the ends of the first liquid cooling grooves (22) located at the center of the bottom of the grinding groove (23). An annular sliding groove (19) is rotatably connected to the outer periphery of each groove liquid cooling pipe (13). The annular sliding grooves (19) are all in sliding fit with the corresponding annular sliding parts (20) to communicate the first waste liquid groove (21) with the annular sliding groove (19). A first recovery pipe (12) is communicated with the bottom wall of each annular sliding groove (19). The first recovery pipes (12) are all communicated with the cooling liquid storage tank (6). The first cold liquid pump (9) is in signal connection with the controller (14).

4. The pretreatment device for detecting grain mycotoxins according to claim 1, wherein: The grinding head assembly includes a top cover (4), the top cover (4) is hinged to a support rod (5), a columnar groove corresponding to the number of grinding grooves (23) is formed in the bottom wall of the top cover (4), through holes (36) are formed in the center of the bottom walls of the columnar grooves, grinding springs (37) are fixedly connected to the bottom walls of the columnar grooves, grinding heads (26) are fixedly connected to the bottom ends of the grinding springs (37), stabilizing rods (38) are fixedly connected to the top walls of the grinding heads (26), and the stabilizing rods (38) respectively pass through the corresponding grinding springs (37) and through holes (36) and extend into the columnar grooves.

5. The pretreatment device for detecting grain mycotoxins according to claim 1, characterized in that: The second liquid cooling assembly includes a second liquid cooling pump (8), the second liquid cooling pump (8) is communicated with a cooling liquid storage tank (6), the output end of the second liquid cooling pump (8) is communicated with head liquid cooling pipes (11) corresponding to the number of grinding heads (26), second liquid cooling grooves (24) are formed inside the grinding heads (26), the second liquid cooling grooves (24) vertically pass through the corresponding stabilizing rods (38) and grinding heads (26) and are laid in a spiral funnel shape inside the bottom wall of the grinding heads (26), the ends of the second liquid cooling grooves (24) are respectively communicated with second waste liquid grooves (39), the second waste liquid grooves (39) respectively pass upward through the grinding heads (26) and stabilizing rods (38), the head liquid cooling pipes (11) are respectively communicated with the tops of the corresponding second liquid cooling grooves (24), the tops of the second waste liquid grooves (39) are respectively communicated with second recovery pipes (10), the second recovery pipes (10) are all communicated with the cooling liquid storage tank (6), and the second liquid cooling pump (8) is in signal connection with a controller (14).

6. The pretreatment device for detecting grain mycotoxins according to claim 1, wherein: The liquid supply assembly includes a liquid supply pump (16), the liquid supply pump (16) is communicated with an extraction liquid storage tank (17), the output end of the liquid supply pump (16) is communicated with a liquid supply pipe (15), the liquid supply pipe (15) is communicated with at least 4 liquid distribution pipes (28), liquid distribution valves (29) are arranged on the liquid distribution pipes (28), at least 4 piston cylinders (30) are fixedly connected to the inner top wall of the columnar groove, the piston cylinders (30) in the same columnar groove are distributed around the corresponding stabilizing rods (38), piston blocks (32) are slidably fitted in the piston cylinders (30), a push rod (34) is fixedly connected to one side of each piston block (32) close to the corresponding stabilizing rod (38), a push arc block (35) is fixedly connected to the end of the push rod (34), the push arc blocks (35) in the same columnar groove are distributed on the outer circumference of the corresponding stabilizing rod (38), a return spring (33) is fixedly connected to each piston block (32), the other end of the return spring (33) is fixedly connected to the inner wall of the piston cylinder (30), the return spring (33) surrounds the corresponding push rod (34) on the outer circumference, the liquid distribution pipes (28) are respectively communicated with the corresponding piston cylinders (30), the piston cylinders (30) are all communicated with liquid spraying pipes (31), the liquid spraying pipes (31) extend downward to the bottom wall of the top cover (4) around the grinding heads (26) and are communicated, the mouths of the liquid spraying pipes (31) all face the surface of the grinding heads (26) and are all provided with silica gel valves, and the liquid supply pump (16) and the liquid distribution valves (29) are both in signal connection with the controller (14).

7. The pretreatment device for detecting grain mycotoxins according to claim 5, wherein: The head liquid cooling pipes (11) and the second recovery pipes (10) are both made of flexible materials.

8. The pretreatment device for detecting grain mycotoxins according to claim 4, characterized in that: The bottom surfaces of the grinding heads (26) are all provided with spirally arranged stripes (27) in neat rows, and the inner walls of the grinding grooves (23) are all covered with a grinding layer.

9. The pretreatment device for detecting grain mycotoxins according to claim 1, characterized in that: A sealing layer (2) that cooperates with the top wall of the base (1) is laid on the bottom wall of the top cover (4).

10. The pretreatment device for detecting grain mycotoxins according to claim 1, characterized in that: Refrigerators (7) are fixedly connected to both the extraction liquid storage tank (17) and the cooling liquid storage tank (6) for precooling the extraction solvent and cooling the cooling liquid. A locking member (3) that cooperates with the base (1) is provided on one side of the top cover (4), and the refrigerators (7) are all signal-connected to the controller (14).

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