Biological bacterial fertilizer containing morchella esculenta nutrition bag waste as well as preparation method and application of biological bacterial fertilizer
The biological bacteria fertilizer was prepared by dynamic temperature-controlled fermentation of morel nutritional bag waste and pond silt combined with composite microbial bacteria, which solved the problem of single function of microbial fertilizer and the treatment of nutrient bag waste, and achieved fruit and vegetable growth promotion and anti-pest effects.
Patent Information
- Application Number
- CN202510486950.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-04-18
- Publication Date
- 2025-07-22
- Estimated Expiration
- Not applicable · inactive patent
AI Technical Summary
The existing microbial bacteria fertilizer has a single function, and it is difficult to take into account the multiple functional needs of organic matter degradation, heavy metal passivation, nutrient activation and anti-response induction. At the same time, the waste treatment of edible fungi nutrition bags has caused environmental pollution.
Morel nutrition bag waste is used to combine complex microbial bacteria with pond silt, including Bacillus subtilis, Bacillus jelly-like, Pseudomonas rhizome, Trichoderma harziana and Phosphorus lysate. The biological bacteria fertilizer is prepared through dynamic temperature-controlled fermentation to promote the growth of fruit and vegetable plants and resist pests and diseases.
Biological bacteria fertilizer promotes the growth of fruit and vegetable plants, improves yield and quality, enhances the ability to resist diseases and pests, solves the problem of waste disposal of nutrient bags, and improves resource utilization.
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Abstract
Description
Technical Field
[0001] The present invention relates to the technical field of microbial fertilizers, and particularly relates to a biological bacterial fertilizer containing waste of Morchella esculenta nutrient bags, a preparation method thereof, and an application thereof. Background Art
[0002] In recent years, with the rapid development of the edible mushroom industry, large amounts of nutrient bag wastes have been generated from the large-scale cultivation of high-value-added edible mushrooms such as Morchella esculenta. Nutrient bags are usually processed from agricultural and forestry by-products such as wheat and rice husks. After inoculating mycelium to complete nutrient supply, the residues still contain incompletely degraded lignocellulose, mycelial residues, extracellular enzymes, and secondary metabolites. At present, these waste nutrient bags are mainly treated by landfill or incineration. However, landfill is likely to cause soil leachate pollution, and incineration will release harmful gases (such as dioxins) and cause waste of resources.
[0003] For microbial bacterial fertilizers, existing microbial bacterial fertilizers mostly adopt single strains or combinations of strains with repeated functions, and there are certain functional short boards in the application process, and may not be able to take into account multiple functional requirements such as organic matter degradation, heavy metal passivation, nutrient activation, and stress resistance induction.
[0004] Based on the above, it is very promising to obtain a bacterial fertilizer that can effectively utilize waste of edible mushroom nutrient bags and simultaneously meet the multiple functional requirements of microbial bacterial fertilizers. Summary of the Invention
[0005] The purpose of the present invention is to provide a biological bacterial fertilizer containing waste of Morchella esculenta nutrient bags, which can effectively promote the growth and development of the root systems of fruit and vegetable plants, improve the yield and quality of fruits and vegetables, and enhance the ability of fruit and vegetable plants to resist the occurrence of diseases and pests.
[0006] In order to achieve the above-mentioned invention purpose, the present invention provides the following technical solutions:
[0007] The present invention provides a biological bacterial fertilizer containing waste of Morchella esculenta nutrient bags, comprising the following raw materials in parts by weight: 70 - 90 parts of waste of Morchella esculenta nutrient bags, 15 - 25 parts of pond sludge, and 0.08 - 0.15 parts of compound microbial bacteria.
[0008] Preferably, the waste of Morchella esculenta nutrient bags is composed of wheat and rice husks in a mass ratio of (15 - 17):(3 - 5).
[0009] Preferably, the pond sludge is composed of excrement, residual feed, and sediment in a mass ratio of (0.55 - 0.65):(0.05 - 0.15):(0.25 - 0.35).
[0010] Preferably, the composite microbial bacteria include Bacillus subtilis, Bacillus mucilaginosus, Rhodopseudomonas palustris, Trichoderma harzianum, and phosphate-solubilizing bacteria in a mass ratio of (4-6):(5-6):(4-5):(3-4):(1-2).
[0011] The present invention also provides a method for preparing the biological bacterial fertilizer, which includes the following steps:
[0012] (1) Mix the morchella nutrient bag waste, pond sludge, and composite microbial bacteria, stack them to obtain a mixed material stack, then cover it with a film and ferment;
[0013] (2) When the temperature in the central area of the mixed material stack reaches 48-52 °C, break the stack, then stack it again, cover it with a film and ferment;
[0014] (3) Repeat step (2) 2-3 times, and stop when the temperature in the central area of the mixed material stack is 38-42 °C.
[0015] Preferably, the moisture content of the mixed material stack in step (1) is 45-55%.
[0016] Preferably, a black film is used for covering the film.
[0017] The present invention also provides the application of the biological bacterial fertilizer in promoting the growth of fruit and vegetable plants and increasing the yield of fruits and vegetables.
[0018] The present invention also provides the application of the biological bacterial fertilizer in improving the quality of fruits and vegetables.
[0019] The present invention also provides the application of the biological bacterial fertilizer in enhancing the stress resistance of fruit and vegetable plants.
[0020] By adopting the above technical solutions, the present invention has the following beneficial effects:
[0021] 1. In the technical solution of the present invention, the morchella nutrient bag waste and pond sludge are used synergistically, providing sufficient and rich nutrients for the growth and development of fruit and vegetable plants; the combined use of several bacteria such as Bacillus subtilis, Bacillus mucilaginosus, Rhodopseudomonas palustris, Trichoderma harzianum, and phosphate-solubilizing bacteria can synergistically increase the efficiency, significantly promote the growth of fruit and vegetable plants, increase the yield and quality of fruits and vegetables, and enhance the pest and disease resistance of fruit and vegetable plants.
[0022] 2. In the technical solution of the present invention, a dynamic temperature control fermentation process is adopted, which can optimize the microbial activity and organic matter degradation efficiency involved in the production process of the biological bacterial fertilizer, and can ensure the fermentation effect and the product quality of the biological bacterial fertilizer.
[0023] 3. The raw materials such as morchella nutrient bag waste and pond sludge used in the technical solution of the present invention have low costs, and also solve the environmental problem that it is difficult to handle the nutrient bags discarded everywhere after morchella cultivation, improving the utilization rate of resources. Detailed implementation mode
[0024] The present invention provides a biological bacterial fertilizer containing waste of Morchella nutrient bags, including waste of Morchella nutrient bags, pond sludge and composite microbial bacteria.
[0025] In the present invention, the weight parts of the waste of Morchella nutrient bags are preferably 70-90 parts, further preferably 75-85 parts, and still further preferably 80 parts; the weight parts of the pond sludge are preferably 15-25 parts, further preferably 18-22 parts, and still further preferably 20 parts; the weight parts of the composite microbial bacteria are preferably 0.08-0.15 parts, further preferably 0.09-0.12 parts, and still further preferably 0.1 part.
[0026] In the present invention, the waste of Morchella nutrient bags is generated after Morchella planting. Remove the packaging of the non-sterile-infected nutrient bags generated after Morchella planting, and collect the substances in the bags to obtain the waste of Morchella nutrient bags. The waste of Morchella nutrient bags in the present invention is preferably composed of components including wheat and rice husks, and the mass ratio of wheat to rice husks is preferably (15-17):(3-5), further preferably (15.5-16.5):(3.5-4.5), and still further preferably 16:4.
[0027] In the present invention, the pond sludge is preferably composed of components including excrement, residual feed and sediment, and the mass ratio of excrement, residual feed and sediment is preferably (0.55-0.65):(0.05-0.15):(0.25-0.35), further preferably (0.58-0.62):(0.08-0.12):(0.28-0.32), and still further preferably 0.6:0.1:0.3.
[0028] In the present invention, the composite microbial bacteria preferably include Bacillus subtilis, Bacillus mucilaginosus, Rhodopseudomonas palustris, Trichoderma harzianum and phosphate-solubilizing bacteria, and the mass ratio of Bacillus subtilis, Bacillus mucilaginosus, Rhodopseudomonas palustris, Trichoderma harzianum and phosphate-solubilizing bacteria is preferably (4-6):(5-6):(4-5):(3-4):(1-2), further preferably (4.5-5.5):(5.2-5.8):(4.2-4.8):(3.3-3.9):(1.2-1.8), and still further preferably 5:5.5:4.5:3.8:1.5. The combined use of the several bacteria of Bacillus subtilis, Bacillus mucilaginosus, Rhodopseudomonas palustris, Trichoderma harzianum and phosphate-solubilizing bacteria in the present invention can significantly synergistically increase the efficiency.
[0029] The present invention also provides a preparation method of the biological bacterial fertilizer, including the following steps:
[0030] (1) Mix the waste of morel nutrient bags, pond sludge and compound microbial bacteria, stack them to obtain a mixed material pile, then cover it with a film and ferment.
[0031] (2) When the temperature in the central area of the mixed material pile reaches 48 - 52 °C, break the pile, then stack it again, cover it with a film and ferment.
[0032] (3) Repeat step (2) 2 - 3 times, and stop when the temperature in the central area of the mixed material pile is 38 - 42 °C.
[0033] In the present invention, the waste of morel nutrient bags, pond sludge and compound microbial bacteria are mixed and stirred evenly, and then stacked to obtain a mixed material pile. The water content of the mixed material pile in the present invention is preferably 45 - 55%, further preferably 48 - 52%, and even more preferably 50%.
[0034] In the present invention, the mixed material pile is covered with a film, preferably covered with a black film. This black film can effectively absorb heat to achieve a heat preservation effect; it can also block light; and inhibit miscellaneous bacteria.
[0035] In the present invention, after covering with a film, fermentation is carried out. When the temperature in the central area of the mixed material pile reaches 48 - 52 °C, break the pile, further preferably to 49 - 51 °C, and even more preferably to 50 °C; then stack it again, cover it with a film and ferment. Repeat the above processes of breaking the pile, re - stacking, covering with a film and fermentation 2 - 3 times, and then stop fermenting when the temperature in the central area of the mixed material pile is 38 - 42 °C, further preferably to 39 - 41 °C, and even more preferably to 40 °C.
[0036] The present invention also provides the application of the biological bacterial fertilizer in promoting the growth of fruit and vegetable plants and increasing the yield of fruits and vegetables.
[0037] The present invention also provides the application of the biological bacterial fertilizer in improving the quality of fruits and vegetables.
[0038] The present invention also provides the application of the biological bacterial fertilizer in enhancing the stress resistance of fruit and vegetable plants.
[0039] In the present invention, the types of fruits and vegetables preferably include ponkan oranges, tomatoes, pakchoi, and grapes.
[0040] The following combines examples to elaborate in detail on the technical solutions provided by the present invention, but they cannot be construed as limiting the protection scope of the present invention.
[0041] The Bacillus subtilis and phosphate - solubilizing bacteria in the present invention are purchased from Shandong Yihao Biotechnology Co., Ltd.; the Bacillus mucilaginosus and Trichoderma harzianum are purchased from Weifang Ruichen Biotechnology Co., Ltd.; the Rhodopseudomonas palustris is purchased from Wuhan Beileye Biomedical Technology Co., Ltd.
[0042] Example 1
[0043] A biological bacterial fertilizer containing waste of Morchella esculenta nutrient bags, with raw materials including 80 parts of waste of Morchella esculenta nutrient bags, 20 parts of pond sludge, and 0.1 part of compound microbial bacteria; the waste of Morchella esculenta nutrient bags is composed of wheat and rice husks with a mass ratio of 16:4, the pond sludge is composed of excrement, residual feed, and sediment with a mass ratio of 0.6:0.1:0.3, and the compound microbial bacteria is composed of Bacillus subtilis, Bacillus mucilaginosus, Rhodopseudomonas palustris, Trichoderma harzianum, and phosphate-solubilizing bacteria with a mass ratio of 5:5.5:4.5:3.8:1.5;
[0044] The preparation method of the above biological bacterial fertilizer comprises the following steps:
[0045] (1) Mix the waste of Morchella esculenta nutrient bags, pond sludge, and compound microbial bacteria evenly, then stack to obtain a mixed material pile with a moisture content of 50%, and then cover it with a black film and ferment;
[0046] (2) When the temperature in the central area of the mixed material pile reaches 50 °C, break the pile, then stack again, cover with a black film, and ferment;
[0047] (3) After repeating step (2) twice, stop fermentation when the temperature in the central area of the mixed material pile is 40 °C.
[0048] Example 2
[0049] A biological bacterial fertilizer containing waste of Morchella esculenta nutrient bags, with raw materials including 70 parts of waste of Morchella esculenta nutrient bags, 25 parts of pond sludge, and 0.15 part of compound microbial bacteria; the waste of Morchella esculenta nutrient bags is composed of wheat and rice husks with a mass ratio of 15:5, the pond sludge is composed of excrement, residual feed, and sediment with a mass ratio of 0.65:0.15:0.25, and the compound microbial bacteria is composed of Bacillus subtilis, Bacillus mucilaginosus, Rhodopseudomonas palustris, Trichoderma harzianum, and phosphate-solubilizing bacteria with a mass ratio of 4:6:4:3:2;
[0050] The preparation method of the above biological bacterial fertilizer comprises the following steps:
[0051] (1) Mix the waste of Morchella esculenta nutrient bags, pond sludge, and compound microbial bacteria evenly, then stack to obtain a mixed material pile with a moisture content of 45%, and then cover it with a black film and ferment;
[0052] (2) When the temperature in the central area of the mixed material pile reaches 52 °C, break the pile, then stack again, cover with a black film, and ferment;
[0053] (3) After repeating step (2) twice, stop fermentation when the temperature in the central area of the mixed material pile is 42 °C.
[0054] Example 3
[0055] A biological bacterial fertilizer containing the waste of Morchella esculenta nutrient bags, with the raw materials being 90 parts of the waste of Morchella esculenta nutrient bags, 15 parts of pond sludge, and 0.08 part of compound microbial bacteria; the waste of Morchella esculenta nutrient bags is composed of wheat and rice husks with a mass ratio of 17:3, the pond sludge is composed of excrement, residual feed, and sediment with a mass ratio of 0.55:0.05:0.35, and the compound microbial bacteria are composed of Bacillus subtilis, Bacillus mucilaginosus, Rhodopseudomonas palustris, Trichoderma harzianum, and phosphate-solubilizing bacteria with a mass ratio of 6:5:5:4:1;
[0056] The preparation method of the above biological bacterial fertilizer comprises the following steps:
[0057] (1) Mix the waste of Morchella esculenta nutrient bags, pond sludge, and compound microbial bacteria evenly, then stack them to obtain a mixed material pile with a moisture content of 55%, and then cover it with a black film and ferment;
[0058] (2) When the temperature in the central area of the mixed material pile reaches 48 °C, break the pile, then stack it again, cover it with a black film, and ferment;
[0059] (3) After repeating step (2) three times, stop fermentation when the temperature in the central area of the mixed material pile is 38 °C.
[0060] Comparative Example 1
[0061] Same as Example 1, only replace "80 parts of the waste of Morchella esculenta nutrient bags" with "20 parts of the waste of Morchella esculenta nutrient bags".
[0062] Comparative Example 2
[0063] Same as Example 1, only replace "the compound microbial bacteria are composed of Bacillus subtilis, Bacillus mucilaginosus, Rhodopseudomonas palustris, Trichoderma harzianum, and phosphate-solubilizing bacteria with a mass ratio of 5:5.5:4.5:3.8:1.5" with "the compound microbial bacteria are composed of Bacillus subtilis, Bacillus mucilaginosus, Rhodopseudomonas palustris, Trichoderma harzianum, and phosphate-solubilizing bacteria with a mass ratio of 1:3:4.5:1:0.5".
[0064] Comparative Example 3
[0065] Same as Example 1, only replace "the compound microbial bacteria are composed of Bacillus subtilis, Bacillus mucilaginosus, Rhodopseudomonas palustris, Trichoderma harzianum, and phosphate-solubilizing bacteria with a mass ratio of 5:5.5:4.5:3.8:1.5" with "the compound microbial bacteria are composed of Bacillus subtilis, Rhodopseudomonas palustris, and Trichoderma harzianum with a mass ratio of 5:5.5:4.5".
[0066] Comparative Example 4
[0067] Same as Example 1, only replace "(2) When the temperature in the central area of the mixed material pile reaches 50°C, break the pile, then stack it again, cover it with a black film, and ferment; (3) After repeating step (2) twice, stop fermenting when the temperature in the central area of the mixed material pile reaches 40°C" with "Stop fermenting when the temperature in the central area of the mixed material pile reaches 50°C".
[0068] Test Example 1
[0069] Select a planting field in Lugao Village, Yaodu Town, Qingbaijiang District to plant tomatoes. Select tomato varieties that are widely planted locally and have good adaptability. The seedlings are sourced from the Chenxiang Agricultural and Trade Seedling Market in Qingbaijiang District. The test is set up with 6 groups, including the Example 1 group, the Comparative Example 1 group, the Comparative Example 2 group, the Comparative Example 3 group, the Comparative Example 4 group, and the control group. Each group has 3 replicates, for a total of 18 planting plots. The area of each planting plot is 2m 2 , and according to the standard of planting 2000 holes per mu, each plot plants 6 holes, and each hole plants 2 plants. After the planting survives, leave 1 strong plant per hole. The tomato planting period is from May 14th to August 20th, 2024.
[0070] The control group is applied with a traditional special compound fertilizer for vegetable planting, with a nitrogen-phosphorus-potassium ternary compound fertilizer content (N:P2O5:K2O = 15:15:15); the other groups are respectively applied with the corresponding prepared bio-fertilizer. At the time of planting, 0.05 kg is applied and mixed evenly with the soil in the hole as the base fertilizer. After planting, immediately water with root-fixing water, and water once every other day until survival. Then, water according to the soil humidity situation; 0.05 kg / hole is top-dressed with water 15 days after planting, and 0.05 kg / hole is top-dressed when the first young fruits appear. That is, the total fertilizer application amount per plot is 0.15 kg.
[0071] (I) Soil physical and chemical properties
[0072] Take soil samples before planting and during the fruiting period respectively, and conduct soil physical and chemical index tests. The physical and chemical indexes include organic matter content, available nitrogen content, available phosphorus content, and available potassium content. The detection method refers to "Soil Agricultural Chemistry Analysis" (Bao Shidan. Soil Agricultural Chemistry Analysis [M]. Beijing: China Agricultural Press.).
[0073] Before planting, the organic matter content of the soil is 21.57 g / kg, the available nitrogen content is 78.11 mg / kg, the available phosphorus content is 33.95 mg / kg, and the available potassium content is 59.02 mg / kg.
[0074] Table 1 Soil physical and chemical index conditions of different treatment groups before planting and during the fruiting period
[0075]
[0076] As can be seen from Table 1, compared with the control group, the physical and chemical indexes of the soil in the result period of the groups of Comparative Examples 1-4 and Example 1 increased significantly, and among them, the improvement in Example 1 group was relatively more significant. That is to say, it shows that the biological bacterial fertilizer described in the present invention can effectively improve the physical and chemical properties of the soil.
[0077] (2) Plant growth indexes
[0078] 30 days after the tomato seedlings were planted, 3 plants were randomly selected at fixed points in each plot to measure the growth indexes such as plant height (main stem height), stem diameter (main stem diameter 2 cm away from the soil), and internode number. The results are shown in Table 2.
[0079] Table 2 Growth indexes of tomato plants in different treatment groups
[0080] Group Plant height / cm Stem diameter / mm Number of internodes / each Example 1 group 94 65 21 Control 1 group 84 47 16 Control 2 group 90 61 19 Control 3 group 88 54 19 Control 4 group 85 49 17 Control group 73 32 13
[0081] As can be seen from Table 2, the growth effects of plant height, stem diameter, and internode number of tomato plants treated with the biological bacterial fertilizer described in the present invention were relatively the most significant.
[0082] (3) Yield
[0083] After the tomatoes were mature, they were harvested and weighed, and the yield per plant and the mass of a single fruit were counted.
[0084] Table 3 Yield of tomatoes in different treatment groups
[0085] Group Yield per plant / kg Single fruit mass / g Example 1 group 1.69 95.35 Control 1 group 1.37 88.14 Control 2 group 1.61 91.07 Control 3 group 1.53 89.56 Control 4 group 1.42 86.33 Control group 1.25 80.20
[0086] As can be seen from Table 3, the tomatoes in the groups of Comparative Examples 1-4 and Example 1 were higher than the control group in terms of yield per plant and average fruit set number. Among them, the tomato yield in Example 1 group was relatively the highest.
[0087] (4) Quality
[0088] After the tomatoes were mature, 3 fruits with similar sizes were selected from each replicate for quality determination, including soluble solid content, organic acid content, and VC content. The soluble solid content was measured by an ATAGO hand-held refractometer, the organic acid content was measured by sodium hydroxide titration method (Li Xiaofang, Zhang Zhiliang, 2016. Experimental Guidance for Plant Physiology, Beijing: Higher Education Press.), and the VC content was measured by molybdenum blue colorimetry (Li Hesheng. 2000. Principles and Techniques of Plant Physiological and Biochemical Experiments. Beijing: Higher Education Press).
[0089] Table 4 Quality indexes of tomatoes in different treatment groups
[0090] Group Soluble solids(%) Organic acid(g / L) VC(mg / kg) Example 1 group 5.84 1.28 112.05 Control 1 group 5.45 1.19 90.34 Control 2 group 5.70 1.26 109.82 Control 3 group 5.59 1.23 102.14 Control 4 group 5.50 1.17 95.52 Control group 5.21 1.11 78.23
[0091] As can be seen from Table 4, compared with the control group, the soluble solids content, organic acid content, and VC content of tomato fruits in Example 1 and Comparative Examples 1-4 were all significantly increased.
[0092] (V) Pest and disease control
[0093] Investigate and count the number of plants with tomato root rot. Taking the browning of the plant roots or the death of the plant as the standard, calculate the incidence rate: Incidence rate = Number of diseased plants / Total number of plants × 100%.
[0094] Table 5 Incidence of tomato root rot in different treatment groups
[0095] Group Incidence rate(%) Example 1 group 1.67 Control 1 group 6.15 Control 2 group 2.34 Control 3 group 3.01 Control 4 group 5.27 Control group 12.05
[0096] As can be seen from Table 5, the incidence rate of tomato root rot in Example 1 group was the lowest, and the plants showed relatively the best performance. That is to say, the biological bacterial fertilizer described in the present invention has a certain effect on preventing and controlling pests and diseases.
[0097] In summary, it can be seen that the biological bacterial fertilizer prepared by the technical solution of the present invention has low cost and rich nutrient elements, can effectively promote the growth and development of the roots of fruit and vegetable plants, improve the yield and quality of fruits and vegetables, and enhance the ability of fruit and vegetable plants to resist the occurrence of pests and diseases; at the same time, it also solves the environmental problem that the nutrient bags are discarded everywhere and difficult to handle after the cultivation of Morchella esculenta, and improves the utilization rate of resources.
[0098] The above are only the preferred embodiments of the present invention. It should be noted that for those of ordinary skill in the art, without departing from the principle of the present invention, several improvements and modifications can be made, and these improvements and modifications should also be regarded as the protection scope of the present invention.
Claims
1. A biological bacterial fertilizer containing the waste of Morchella nutrient bags, characterized in that, It comprises raw materials in the following parts by weight: 70 - 90 parts of Morchella esculenta nutrient bag waste, 15 - 25 parts of pond sludge, and 0.08 - 0.15 parts of compound microbial bacteria.
2. The biological bacterial fertilizer according to claim 1, characterized in that, The Morchella esculenta nutrient bag waste is composed of wheat and chaff with a mass ratio of (15 - 17):(3 - 5); The pond sludge is composed of excrement, residual feed, and sediment with a mass ratio of (0.55 - 0.65):(0.05 - 0.15):(0.25 - 0.35).
3. The biological bacterial fertilizer according to claim 1, characterized in that, The compound microbial bacteria include Bacillus subtilis, Bacillus mucilaginosus, Rhodopseudomonas palustris, Trichoderma harzianum, and phosphate - solubilizing bacteria with a mass ratio of (4 - 6):(5 - 6):(4 - 5):(3 - 4):(1 - 2).
4. The preparation method of the biological bacterial fertilizer according to any one of claims 1-3, characterized in that, It includes the following steps: (1) Mix the Morchella esculenta nutrient bag waste, pond sludge, and compound microbial bacteria and stack them to obtain a mixed material stack, then cover it with a film and ferment; (2) When the temperature in the central area of the mixed material stack reaches 48 - 52 °C, break the stack, then stack it again, cover it with a film and ferment; (3) Repeat step (2) 2 - 3 times and stop when the temperature in the central area of the mixed material stack is 38 - 42 °C.
5. The preparation method according to claim 4, wherein, The moisture content of the mixed material stack in step (1) is 45 - 55%.
6. The preparation method according to claim 4, characterized in that, Cover it with a black film.
7. The application of the biological bacterial fertilizer according to any one of claims 1 - 3 in promoting the growth of fruit and vegetable plants and increasing the yield of fruits and vegetables.
8. The application of the biological bacterial fertilizer according to any one of claims 1 - 3 in improving the quality of fruits and vegetables.
9. The application of the biological bacterial fertilizer according to any one of claims 1 - 3 in enhancing the stress resistance of fruit and vegetable plants.
Citation Information
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