Nucleic acid purification and amplification device

Abstract

The invention discloses a nucleic acid purification and amplification device, and relates to the technical field of nucleic acid purification. Comprising a tube bin, and the tube bin comprises a sample bin; the filtering column is arranged in the pipe bin, and the filtering column is used for purifying a sample; threads are arranged on the outer portion of the screw cap, and the screw cap is in threaded connection with the pipe bin; the amplification module comprises an amplification bin, and the amplification bin is used for detecting the purified sample; according to the nucleic acid purification and amplification device, after a sample is added into the pipe bin, the sample can flow into the amplification module after puncturing the sealing ring, and the sample is controlled to enter the amplification bin from the sample bin through the negative pressure in the amplification module; the sample amplification bin is used for detecting the sample in the amplification bin to obtain the detection result of the sample, so that the portable requirement of medical detection is met, and the operation is convenient and rapid.

Description

Technical Field

[0001] This application relates to the technical field of nucleic acid purification, and specifically provides a nucleic acid purification and amplification device. Background Art

[0002] Nucleic acid purification refers to the process of separating and extracting DNA or RNA from samples, mainly used in molecular biology experiments in scientific research and clinical diagnosis. This process can effectively remove corticosteroids, proteins, carbohydrates, lipids, and other impurities in the samples, thereby obtaining pure nucleic acids. In related POCT nucleic acid detection technologies, for some medical tests, such as pathogen nucleic acid detection, the requirements for simple operation, portability, and low cost of detection are increasing.

[0003] In the prior art, there are products such as microfluidic cartridges and microfluidic chips, which have many consumable components, complex assembly processes, high prices, large volumes, and technical routes that usually include rotary valve control, mechanical valve control, paraffin valve control, etc., and have very high requirements for the precise control of the instrument, and cannot meet the existing detection needs in many scenarios.

[0004] Application Content

[0005] To overcome the problems of many consumable components, complex assembly processes, high prices, large volumes, technical routes that usually include rotary valve control, mechanical valve control, paraffin valve control, etc., and very high requirements for the precise control of the instrument, and cannot meet the existing detection needs in many scenarios, this application provides a nucleic acid purification and amplification device, including a tube bin, and the tube bin includes a sample bin;

[0006] A filter column, which is arranged inside the tube bin and is used to purify the sample;

[0007] A screw cap, with threads on its outer part, and the screw cap is threadedly connected to the tube bin;

[0008] An amplification module, which includes an amplification bin, and the amplification bin is used to detect the purified sample;

[0009] A sealing plug, which is arranged inside the tube bin and is used to control the connection between the tube bin and the amplification module, and the sample flows into the amplification module after entering the sealing plug from the tube bin;

[0010] A base, which is connected to the amplification module.

[0011] Preferably, the tube bin further includes:

[0012] A first buckle, which is arranged outside the tube bin and is used to connect the tube bin and the amplification module;

[0013] Flow channel column, which is arranged inside the tube bin.

[0014] Preferably, the tube bin further includes:

[0015] The first flow channel, which is opened inside the flow channel column, is communicated with the sample bin, and the filter column is installed inside the first flow channel;

[0016] The first sealing bin, which is opened inside the tube bin, is used to cooperate with the sealing plug to complete the sealing work.

[0017] Preferably, the sealing plug includes:

[0018] The diaphragm, which is arranged inside the sealing plug;

[0019] The second flow channel, which is opened inside the sealing plug, is installed outside the flow channel column;

[0020] The third flow channel, which is opened inside the sealing plug.

[0021] Preferably, the amplification module further includes:

[0022] The second sealing bin, which is opened inside the amplification module, is installed outside the sealing plug;

[0023] The amplification bin is arranged below the second sealing bin and is communicated with the second sealing bin.

[0024] Preferably, the amplification module further includes:

[0025] The fourth flow channel, which is opened inside the second sealing bin;

[0026] The thimble, which is arranged inside the fourth flow channel, and the end of the thimble away from the fourth flow channel is installed inside the third flow channel. The thimble is used to puncture the diaphragm so that the sample flows into the amplification bin.

[0027] Preferably, the amplification module further includes:

[0028] The first card group, which is opened on the side of the amplification module and cooperates with the first buckle;

[0029] The second card group, which is opened on the side of the amplification module and cooperates with the first buckle.

[0030] Preferably, the amplification module further includes:

[0031] The third card group, which is opened on the side of the amplification module;

[0032] The fourth card deck is provided on the side of the amplification module.

[0033] Preferably, the base includes:

[0034] A second buckling position installed inside the base and cooperating with the third card deck;

[0035] A third buckling position installed inside the base and cooperating with the fourth card deck.

[0036] This application provides a nucleic acid purification and amplification device, which has the following beneficial effects:

[0037] 1. For this nucleic acid purification and amplification device, through the settings of the tube bin, the amplification module and the sealing plug, the purification and collection and detection of the sample solution are completed. By using the sealing plug to connect the tube bin and the amplification module, the connection relationship is simple. When the sealing plug and the thimble are in different states, the overall sealing condition of the device is different.

[0038] 2. For this nucleic acid purification and amplification device, through the settings of the first buckling position, the second buckling position, the third buckling position, the first card deck, the second card deck, the third card deck and the fourth card deck, the first card deck cooperates with the first buckling position, the second card deck cooperates with the first buckling position, the second buckling position cooperates with the third card deck, and the third buckling position cooperates with the fourth card deck. With each press of the knob, the first buckling position, the second buckling position and the third buckling position all move down one card position to cooperate with the sealing work between the thimble and the flow channel.

[0039] 3. For this nucleic acid purification and amplification device, through the settings of the flow channel column, the first flow channel and the filtration column, during the process of the sample solution flowing through the first flow channel, the sample solution contacts the filtration column in the first flow channel to perform nucleic acid purification on the sample solution, and the purification process is simple. By setting the flow channel column, the flow channel column is assembled with the second flow channel in the sealing plug, which is convenient for connecting the tube bin and the sealing plug, convenient for the flow of the sample solution, and at the same time convenient for the combination and sealing of the thimble and the flow channel column to seal the entire amplification module.

[0040] 4. For this nucleic acid purification and amplification device, through the settings of the tube bin, the amplification module and the sealing plug, the purification and collection and detection of the sample solution are completed. By pressing the screw cap, the thimble is at different heights in the sealing plug to complete the work of sealing the sample chamber, piercing the diaphragm and sealing the amplification module. And the above work can be completed by pressing the screw cap. Compared with the prior art devices with complex assembly processes, large volumes, and technical routes including rotary valve control, mechanical valve control, and paraffin valve control, it has the advantages of convenient and fast operation process.

[0041] 5. When the screw cap is pressed for the first time, the ejector pin passes through the third flow channel to pierce the diaphragm. After lysis, the sample passes through the first flow channel under the negative pressure in the amplification module, passes through the filter column to purify the sample, completing the purification of the sample solution. Subsequently, the sample passes through the second and third flow channels, then enters the fourth flow channel, and finally flows into the amplification chamber. When the screw cap is pressed for the second time, the ejector pin and the flow channel column are combined to seal, completing the sealing of the entire amplification module. The sealing plug and the amplification module suck the sample solution into the amplification module by applying negative pressure. At the same time, the negative pressure in the amplification module preserves the lyophilized reagent. BRIEF DESCRIPTION OF THE DRAWINGS

[0042] Figure 1 is a schematic diagram of the overall structure of the present application;

[0043] Figure 2 is a sectional view of the tube bin of the present application;

[0044] Figure 3 is a sectional view of the sealing plug of the present application;

[0045] Figure 4 is a schematic diagram of the structure of the filter column of the present application;

[0046] Figure 5 is a schematic diagram of the structure of the screw cap of the present application;

[0047] Figure 6 is a schematic diagram of the structure of the amplification module of the present application;

[0048] Figure 7 is a schematic diagram of the structure of the amplification chamber of the present application;

[0049] Figure 8 is a schematic diagram of the structure of the base of the present application.

[0050] In the figure: 10, tube bin; 11, sample bin; 12, first buckle position; 13, first flow channel; 14, flow channel column; 15, first sealing chamber; 20, sealing plug; 21, diaphragm; 22, second flow channel; 23, third flow channel; 30, filter column; 40, screw cap; 50, amplification module; 51, ejector pin; 52, fourth flow channel; 53, first card group; 54, second card group; 55, third card group; 56, fourth card group; 57, second sealing chamber; 58, amplification chamber; 60, base; 61, second buckle position; 62, third buckle position. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0051] The present application will be further described in detail below in conjunction with the accompanying drawings and specific embodiments. The embodiments of the present application are given for purposes of illustration and description, and are not exhaustive or limit the present application to the disclosed form. Many modifications and variations are obvious to those of ordinary skill in the art. The embodiments are selected and described to better illustrate the principles and practical applications of the present application, and enable those of ordinary skill in the art to understand the present application and thus design various embodiments with various modifications suitable for specific purposes.

[0052] As Figures 1 - 8 shown, the present application provides a technical solution: including a tube bin 10, where sample lysis solution is stored in the tube bin 10, and a sample bin 11 is provided inside the tube bin 10;

[0053] A filter column 30, which is arranged inside the tube bin 10 and is used to purify the sample;

[0054] A screw cap 40, with threads provided on the outside of the screw cap 40, and both the screw cap 40 and the tube bin 10 are unscrewed and screwed shut through the threads;

[0055] An amplification module 50, which includes an amplification chamber 58. Lyophilized bead reagents and paraffin are placed in the amplification chamber 58, and the amplification chamber 58 is used to detect the purified sample;

[0056] A sealing plug 20, which is arranged inside the tube bin 10 and is used to control the connection between the tube bin 10 and the amplification module 50. The sample enters the amplification module 50 after flowing into the sealing plug 20 from the tube bin 10;

[0057] A base 60, which is connected to the amplification module 50.

[0058] After placing the sampling swab or sample solution into the sample bin 11, manually screw on the screw cap 40 to connect the screw cap 40 to the tube bin 10. Subsequently, manually press the screw cap 40 to drive the tube bin 10 to move downward, controlling the sample solution to enter the amplification module 50 through the sample bin 11 and the sealing plug 20, and manually press the screw cap 40 again to seal the amplification module 50.

[0059] The tube bin 10 further includes:

[0060] A first buckle position 12, which is arranged outside the tube bin 10 and is used to connect the tube bin 10 and the amplification module 50;

[0061] A flow channel column 14, which is arranged inside the tube bin 10;

[0062] The first flow channel 13 is opened inside the flow channel column 14. The first flow channel 13 communicates with the sample chamber 11. The filter column 30 is installed inside the first flow channel 13, and the first flow channel 13 and the filter column 30 are assembled together.

[0063] The first sealing chamber 15 is opened inside the tube chamber 10. The first sealing chamber 15 is used to cooperate with the sealing plug 20 to complete the sealing work, and the first sealing chamber 15 and the sealing plug 20 are assembled.

[0064] The sealing plug 20 further includes:

[0065] The diaphragm 21 is arranged inside the sealing plug 20.

[0066] The second flow channel 22 is opened inside the sealing plug 20. The second flow channel 22 is installed outside the flow channel column 14, and the flow channel column 14 and the second flow channel 22 are assembled together.

[0067] The third flow channel 23 is opened inside the sealing plug 20.

[0068] The amplification module 50 further includes:

[0069] The second sealing chamber 57 is opened inside the amplification module 50. The second sealing chamber 57 is installed outside the sealing plug 20. After the second sealing chamber 57 and the lower part of the sealing plug 20 are combined, negative pressure is drawn to seal.

[0070] The amplification chamber 58 is arranged below the second sealing chamber 57, and the amplification chamber 58 communicates with the second sealing chamber 57.

[0071] The fourth flow channel 52 is opened inside the second sealing chamber 57.

[0072] The thimble 51 is arranged inside the fourth flow channel 52. One end of the thimble 51 far from the fourth flow channel 52 is installed inside the third flow channel 23. The third flow channel 23 and the thimble 51 are assembled together. The thimble 51 is used to puncture the diaphragm 21 so that the sample flows into the amplification chamber 58.

[0073] As the tube chamber 10 moves downward, the tube chamber 10 drives the first sealing chamber 15 inside it to move downward. The first sealing chamber 15 squeezes the sealing plug 20 downward. The thimble 51 pierces through the third flow channel 23 to puncture the diaphragm. The lysed sample passes through the first flow channel 13, passes through the filter column 30 to purify the sample, then the sample passes through the second flow channel 22 and the third flow channel 23, then enters the fourth flow channel 52, and finally flows into the amplification chamber 58.

[0074] As the tube chamber 10 moves downward again, the tube chamber 10 drives the first sealing chamber 15 inside it to move downward. The first sealing chamber 15 squeezes the sealing plug 20 downward. The thimble 51 and the flow channel column 14 are combined for sealing to seal the entire amplification module 50.

[0075] Deck 1 53 is provided on the side of the amplification module 50. Deck 1 53 cooperates with the first buckle position 12, and there are three card positions in Deck 1 53;

[0076] Deck 2 54 is provided on the side of the amplification module 50. Deck 2 54 cooperates with the first buckle position 12, and there are three card positions in Deck 2 54.

[0077] Deck 3 55 is provided on the side of the amplification module 50, and there are three card positions in Deck 3 55;

[0078] Deck 4 56 is provided on the side of the amplification module 50, and there are three card positions in Deck 4 56.

[0079] The base 60 further includes:

[0080] The second buckle position 61 is installed inside the base 60, and the second buckle position 61 cooperates with Deck 3 55;

[0081] The third buckle position 62 is installed inside the base 60, and the third buckle position 62 cooperates with Deck 4 56.

[0082] Before using the device, Deck 1 53 cooperates with the first buckle position 12, Deck 2 54 cooperates with the first buckle position 12, the second buckle position 61 cooperates with Deck 3 55, and the third buckle position 62 cooperates with Deck 4 56, and the cooperation positions are all at the first card position at the top of the multiple decks.

[0083] After placing the sample solution, manually press the screw cap 40 to drive the tube bin 10 to move down one card position. At this time, the first buckle position 12 moves down to the second card position between Deck 1 53 and Deck 2 54; the second buckle position 61 moves down to the second card position of Deck 3 55; the third buckle position 62 moves down to the second card position of Deck 4 56. At this time, the sample solution gradually enters the amplification chamber 58.

[0084] Subsequently, manually press the screw cap 40 again to drive the tube bin 10 to move down one more card position. At this time, the first buckle position 12 moves down to the third card position between Deck 1 53 and Deck 2 54; the second buckle position 61 moves down to the third card position of Deck 3 55; the third buckle position 62 moves down to the third card position of Deck 4 56. The sealing work of the amplification chamber 58 is completed.

[0085] The volume in the amplification chamber 58 ranges from 30 - 60 ul, so different solutions can meet the reactions of different amplification reagents.

[0086] Synchronous implementation case. This amplification module 50 can be combined with various numbers of amplification chambers 58, such as one, two, three, four, etc., to meet the requirement that multiple reagent reactions can be carried out in one amplification.

[0087] Working principle: Before using the device, the first card set 53 is matched with the first buckle position 12, the second card set 54 is matched with the first buckle position 12, the second buckle position 61 is matched with the third card set 55, and the third buckle position 62 is matched with the fourth card set 56, and the matching positions are all at the first card position at the top of multiple card sets.

[0088] After putting the sampling swab or sample solution into the sample chamber 11, manually tighten the screw cap 40, and the screw cap 40 is connected to the tube chamber 10. Then manually press the screw cap 40 to drive the tube chamber 10 to move down one card position. At this time, the first buckle position 12 moves down to the second card position between the first card set 53 and the second card set 54; the second buckle position 61 moves down to the second card position of the third card set 55; the third buckle position 62 moves down to the second card position of the fourth card set 56.

[0089] As the tube chamber 10 moves down, the tube chamber 10 drives the internal sealing chamber one 15 to move down. The sealing chamber one 15 squeezes the sealing plug 20 downward, and the thimble 51 pierces the diaphragm through the third flow channel 23. The lysed sample passes through the first flow channel 13, purifies the sample through the filter column 30, then the sample passes through the second flow channel 22 and the third flow channel 23, and then enters the fourth flow channel 52, and finally flows into the amplification chamber 58.

[0090] Then manually press the screw cap 40 again to drive the tube chamber 10 to move down one more card position. At this time, the first buckle position 12 moves down to the third card position between the first card set 53 and the second card set 54; the second buckle position 61 moves down to the third card position of the third card set 55; the third buckle position 62 moves down to the third card position of the fourth card set 56. The sealing work of the amplification chamber 58 is completed.

[0091] As the tube chamber 10 moves down, the tube chamber 10 drives the internal sealing chamber one 15 to move down. The sealing chamber one 15 squeezes the sealing plug 20 downward, and the thimble 51 is combined with the flow channel column 14 for sealing to seal the entire amplification module 50.

[0092] Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art and related fields without creative work should fall within the scope of protection of the present application. The structures, devices, and operation methods not specifically described and explained in the present application, unless otherwise specified and limited, are implemented according to the conventional means in the art.

Claims

1. A nucleic acid purification and amplification device, comprising a tube bin (10), characterized in that: The tube bin (10) includes a sample bin (11); A filter column (30), the filter column (30) is arranged inside the tube bin (10), and the filter column (30) is used to purify the sample; A screw cap (40), the outside of the screw cap (40) is provided with threads, and the screw cap (40) is threadedly connected to the tube bin (10); An amplification module (50), the amplification module (50) includes an amplification chamber (58), and the amplification chamber (58) is used to detect the purified sample; A sealing plug (20), the sealing plug (20) is arranged inside the tube bin (10), and the sealing plug (20) is used to control the connection between the tube bin (10) and the amplification module (50), and the sample enters the sealing plug (20) from the tube bin (10) and then flows into the amplification module (50); A base (60), the base (60) is connected to the amplification module (50).

2. The nucleic acid purification and amplification device according to claim 1, characterized in that: The tube bin (10) further includes: A first buckle position (12), the first buckle position (12) is arranged outside the tube bin (10), and the first buckle position (12) is used to connect the tube bin (10) and the amplification module (50); A flow channel column (14), the flow channel column (14) is arranged inside the tube bin (10).

3. The nucleic acid purification and amplification device according to claim 2, characterized in that: The tube bin (10) further includes: A first flow channel (13), the first flow channel (13) is opened inside the flow channel column (14), the first flow channel (13) is communicated with the sample bin (11), and the filter column (30) is installed inside the first flow channel (13); A first sealing chamber (15), the first sealing chamber (15) is opened inside the tube bin (10), and the first sealing chamber (15) is used to cooperate with the sealing plug (20) to complete the sealing work.

4. The nucleic acid purification and amplification device according to claim 3, characterized in that: The sealing plug (20) includes: A diaphragm (21), the diaphragm (21) is arranged inside the sealing plug (20); A second flow channel (22), the second flow channel (22) is opened inside the sealing plug (20), and the second flow channel (22) is installed outside the flow channel column (14); A third flow channel (23), the third flow channel (23) is opened inside the sealing plug (20).

5. The nucleic acid purification and amplification device according to claim 4, characterized in that: The amplification module (50) further includes: A second sealing chamber (57), the second sealing chamber (57) is opened inside the amplification module (50), and the second sealing chamber (57) is installed outside the sealing plug (20); The amplification chamber (58) is arranged below the second sealing chamber (57), and the amplification chamber (58) is communicated with the second sealing chamber (57).

6. The nucleic acid purification and amplification device according to claim 5, characterized in that: The amplification module (50) further includes: A fourth flow channel (52), the fourth flow channel (52) is opened inside the second sealing chamber (57); A thimble (51), the thimble (51) is arranged inside the fourth flow channel (52), one end of the thimble (51) away from the fourth flow channel (52) is installed inside the third flow channel (23), and the thimble (51) is used to puncture the diaphragm (21) so that the sample flows into the amplification chamber (58).

7. The nucleic acid purification and amplification device according to claim 6, characterized in that: The amplification module (50) further includes: A first card set (53), the first card set (53) is opened on the side of the amplification module (50), and the first card set (53) cooperates with the first buckle (12); A second card set (54), the second card set (54) is opened on the side of the amplification module (50), and the second card set (54) cooperates with the first buckle (12).

8. The nucleic acid purification and amplification device according to claim 7, characterized in that: The amplification module (50) further includes: A third card set (55), the third card set (55) is opened on the side of the amplification module (50); A fourth card set (56), the fourth card set (56) is opened on the side of the amplification module (50).

9. The nucleic acid purification and amplification device according to claim 8, characterized in that: The base (60) includes: A second buckle (61), the second buckle (61) is installed inside the base (60), and the second buckle (61) cooperates with the third card set (55); A third buckle (62), the third buckle (62) is installed inside the base (60), and the third buckle (62) cooperates with the fourth card set (56).

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