Automatic wheat gene sample extraction device

Abstract

The invention provides a wheat gene sample automatic extraction device, which comprises an extraction processing box, supporting foot bases, a sealing door, an automatic control panel group, a vibration crushing box, a cooling sealing cover, an insulating handle, a cooling liquid nitrogen tank and an analysis detection box, the supporting foot bases are respectively in bolted connection with four corners of the lower part of the extraction processing box; the sealing door hinge is connected to the lower side of the front part of the extraction processing box; the automatic control panel set is connected to the upper side of the front portion of the extraction processing box through screws. Liquid nitrogen grinding is combined with ceramic grinding beads, and a vibration motor, a supporting tray, a locking screw, a test tube clamping seat and a constraint clamping hole are utilized, so that high-throughput treatment of multiple wheat samples can be realized at the same time, the DNA extraction cost of a single sample is reduced by 60%, and cells are uniformly broken; and the analysis detection box, the detection probe group, the cracking bin, the communicating pipe and the processor are arranged, so that different tissues such as wheat endosperm (high starch) and leaves (high fiber) can be processed.

Description

Technical Field

[0001] The present invention belongs to the field of agricultural biotechnology, and particularly relates to an automated wheat gene sample extraction device. Background Art

[0002] In the journey of ensuring national food security and rural revitalization, wheat, as the "pillar of the granary", the improvement of its yield and quality has always been the focus of attention of scientists. However, the wheat genome is huge, structurally complex and rich in highly repetitive sequences, which has long hindered the in-depth research and breeding applications of wheat. In terms of individual development of biological tissues, it is the division of fertilized egg cells to produce many cells. These cells are initially the same in morphology, structure and function. Later, through cell differentiation, they gradually form various different morphologies and have different functions. When processing biological tissue samples, a grinder is needed to grind the biological tissue.

[0003] In addition, Chinese Patent Publication No. CN101852695B, with the invention title of a high-throughput tissue grinder, includes a base, at least one working frame and a driving mechanism for driving the working frame. The working frame is connected to the driving mechanism through a crank mechanism. The feature is that a sliding guiding mechanism is arranged on the crank mechanism. However, there are still problems in the existing wheat gene sample extraction, such as low efficiency of the traditional grinding method, uneven cell breakage, large errors in reagent addition accuracy affected by manual operation, and inability to be compatible with different tissue types.

[0004] In view of this, it is very necessary to invent an automated wheat gene sample extraction device. Summary of the Invention

[0005] In order to solve the above technical problems, the present invention provides an automated wheat gene sample extraction device to solve the problems of low efficiency of the traditional grinding method in the existing wheat gene sample extraction, uneven cell breakage, large errors in reagent addition accuracy affected by manual operation, and inability to be compatible with different tissue types.

[0006] An automatic wheat gene sample extraction device, comprising an extraction and processing box, support pedestals, a sealing door, an automatic control panel group, a vibration and pulverization box, a cooling and sealing cover, insulating handles, a coolant nitrogen tank, and an analysis and detection box. The support pedestals are respectively bolted to the four lower corners of the extraction and processing box; the sealing door is hinge-connected to the lower front side of the extraction and processing box; the automatic control panel group is screwed to the upper front side of the extraction and processing box; the vibration and pulverization box is screwed to the top of the extraction and processing box; the cooling and sealing cover is snap-connected to the upper end of the vibration and pulverization box and hinge-connected at the intersection of the rear part; the insulating handles are screwed to the upper right side of the cooling and sealing cover; the coolant nitrogen tank is arranged at the lower left inside of the extraction and processing box; the analysis and detection box is bolted to the right inside position of the extraction and processing box.

[0007] Preferably, a vibration motor is bolted to the lower left inside of the vibration and pulverization box; the output end of the vibration motor is screwed with a support tray, and a test tube holder is clamped on the upper part of the support tray.

[0008] Preferably, an air delivery pipe is connected to the upper part of the coolant nitrogen tank, a control power pump is sleeved on the upper end of the air delivery pipe, and an air supply pipe is sleeved on the output end of the control power pump.

[0009] Preferably, a communication pipe is screwed to the upper end of the analysis and detection box; a cracking chamber is screwed to the upper part of the communication pipe; a processor is welded to the left side of the analysis and detection box.

[0010] Preferably, a detection probe group is inlaid on the left inside of the analysis and detection box, a test tube support bracket is screwed to the right inside of the analysis and detection box, and the test tube support bracket and the detection probe group are arranged in one-to-one correspondence.

[0011] Preferably, locking screws are screwed to both sides of the support tray, a plurality of restraint holes are formed in the upper part of the test tube holder, and the test tube holder is clamped in the support tray and fixed with the locking screws.

[0012] Preferably, the automatic control panel group includes an automatic control panel main body, a touch liquid crystal display screen, indicator lights, function setting buttons, speed adjustment buttons, an emergency stop button, a confirmation and start button, and a power button. The touch liquid crystal display screen is inlaid on the left inside of the automatic control panel main body; the indicator lights are inlaid on the left side of the automatic control panel main body; the function setting buttons, speed adjustment buttons, and emergency stop button are inlaid on the upper right side of the automatic control panel main body from left to right; the confirmation and start button and the power button are inlaid on the lower right side of the automatic control panel main body from left to right.

[0013] Preferably, a storage box is connected to the lower left side inside the cooling and sealing cover by screws, and grinding beads are snap-fitted inside the storage box.

[0014] Preferably, the grinding beads are ceramic grinding beads.

[0015] Preferably, the indicator light is located on the left side of the touch liquid crystal display screen.

[0016] Preferably, the detection probe group integrates a pH sensor (accuracy ±0.2) and a temperature control module (±0.5°C).

[0017] Preferably, the liquid nitrogen coolant tank integrates a liquid nitrogen quick-freezing module (-196°C ± 5°C controllable).

[0018] Preferably, the internal embedded bioinformatics processing unit of the processor has a real-time fluorescence quantitative PCR data analysis SNP marker automatic recognition (applicable to wheat A / B / D genomes) and an extraction quality dynamic feedback control system.

[0019] Compared with the prior art, the beneficial effects of the present invention are as follows: The automatic wheat gene sample extraction device of the present invention uses liquid nitrogen grinding combined with ceramic grinding beads, provides a CTAB improved method to make the polysaccharide removal rate ≥98% (15 - 30% remains in the conventional method), and the OD260 / 280 purity is stably between 1.8 - 2.0; by using a vibration motor, a support tray, locking screws, test tube holders and restraint holes, it can simultaneously achieve high-throughput processing of multiple wheat samples / 90 minutes (the traditional method takes more than 6 hours), the single-sample DNA extraction cost is reduced by 60%, and the cell breakage is uniform; the settings of the analysis and detection box, the detection probe group, the lysis chamber, the connecting pipe and the processor can process different tissues such as wheat endosperm (high starch), leaves (high fiber), etc. (CV value < 5%). BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 is a schematic structural diagram of the present invention.

[0021] Figure 2 is a schematic sectional view of the extraction and processing box of the present invention.

[0022] Figure 3 is a schematic top view of the test tube holder of the present invention.

[0023] Figure 4 is a schematic internal structure diagram of the analysis and detection box of the present invention.

[0024] Figure 5 is a schematic structural diagram of the automatic control panel group of the present invention.

[0025] Figure 6It is a partial structural schematic diagram of the coolant nitrogen tank of the present invention.

[0026] In the figure: 1. Extraction processing box; 2. Support footrest; 3. Sealing door; 4. Automatic control panel group; 41. Automatic control panel main body; 42. Touch liquid crystal display screen; 43. Indicator light; 44. Function setting button; 45. Rotation speed adjustment button; 46. Emergency stop button; 47. Confirmation start button; 48. Power button; 5. Vibration crushing box; 6. Cooling sealing cover; 61. Storage box; 611. Grinding beads; 7. Insulating handle; 8. Support tray; 81. Locking screw; 9. Test tube holder; 91. Constraint card hole; 10. Coolant nitrogen tank; 101. Gas transmission pipe 101; 102. Control power pump; 103. Gas supply pipe; 11. Analysis and detection box; 111. Detection probe group; 112. Test tube support bracket; 12. Pyrolysis chamber; 13. Connecting pipe; 14. Processor; 15. Vibration motor. Specific embodiments

[0027] The present invention will be further described below with reference to the accompanying drawings: Embodiment

[0028] As shown in the attached Figure 1 to the attached Figure 6 figures, the present invention provides an automatic wheat gene sample extraction device, including an extraction processing box 1, a support footrest 2, a sealing door 3, an automatic control panel group 4, a vibration crushing box 5, a cooling sealing cover 6, an insulating handle 7, a coolant nitrogen tank 10 and an analysis and detection box 11. The support footrests 2 are respectively bolted to the four lower corners of the extraction processing box 1; the sealing door 3 is hinge-connected to the lower front side of the extraction processing box 1; the automatic control panel group 4 is screwed to the upper front side of the extraction processing box 1; the vibration crushing box 5 is screwed to the top of the extraction processing box 1; the cooling sealing cover 6 is buckled on the upper end of the vibration crushing box 5 and is hinge-connected at the intersection of the rear part; the insulating handle 7 is screwed to the upper right side of the cooling sealing cover 6; the; the coolant nitrogen tank 10 is arranged at the lower left inside of the extraction processing box 1; the analysis and detection box 11 is bolted to the right inside of the extraction processing box 1.

[0029] In the above implementation scheme, specifically, a vibration motor 15 is bolted to the lower left inside of the vibration crushing box 5; the output end of the vibration motor 15 is screwed to a support tray 8, and a test tube holder 9 is clamped on the upper part of the support tray 8.

[0030] In the above-described embodiments, specifically, an air delivery pipe 101 is connected to the upper part of the coolant nitrogen tank 10, a control power pump 102 is sleeved on the upper end of the air delivery pipe 101, and an air supply pipe 103 is sleeved on the output end of the control power pump 102.

[0031] In the above-described embodiments, specifically, a communication pipe 13 is threadedly connected to the upper end of the analysis and detection box 11; a cracking chamber 12 is threadedly connected to the upper part of the communication pipe 13; a processor 14 is welded to the left side of the analysis and detection box 11; a detection probe group 111 is embedded on the left side inside the analysis and detection box 11, and a test tube support bracket 112 is screwed to the right side inside the analysis and detection box 11, and the test tube support bracket 112 and the detection probe group 111 are arranged in one-to-one correspondence.

[0032] In the above-described embodiments, specifically, locking screws 81 are threadedly connected to both sides of the support tray 8, a plurality of restraint holes 91 are formed in the upper part of the test tube holder 9, and the test tube holder 9 is clamped in the support tray 8 and fixed with the locking screws 81.

[0033] In the above-described embodiments, specifically, the automated control panel group 4 includes an automatic control panel main body 41, a touch liquid crystal display screen 42, an indicator light 43, a function setting button 44, a speed adjustment button 45, an emergency stop button 46, a confirmation start button 47, and a power button 48. The touch liquid crystal display screen 42 is embedded on the left side inside the automatic control panel main body 41; the indicator light 43 is embedded on the left side of the automatic control panel main body 41; the function setting button 44, the speed adjustment button 45, and the emergency stop button 46 are sequentially embedded on the upper right side of the automatic control panel main body 41 from left to right; the confirmation start button 47 and the power button 48 are sequentially embedded on the lower right side of the automatic control panel main body 41 from left to right.

[0034] In the above-described embodiments, specifically, a storage box 61 is screwed to the lower left side inside the cooling sealing cover 6, and a grinding bead 611 is clamped in the storage box 61; the grinding bead 611 is a ceramic grinding bead.

[0035] In the above-described embodiments, specifically, the indicator light 43 is located on the left side of the touch liquid crystal display screen 42.

[0036] In the above-described embodiments, specifically, the detection probe group 111 integrates a pH sensor (accuracy ±0.2) and a temperature control module (±0.5°C); the coolant nitrogen tank 10 integrates a liquid nitrogen quick-freezing module (-196°C ± 5°C controllable).

[0037] In the above embodiments, specifically, the internal embedded bioinformatics processing unit of the processor 14, real-time fluorescence quantitative PCR data analysis SNP marker automatic recognition (applicable to wheat A / B / D genomes) and extraction quality dynamic feedback control system.

[0038] The working process of the present invention is as follows: Insert the test tube containing the wheat tissue sample into the restraint card hole 91, add an appropriate amount of grinding beads 611 into the test tube, fasten the cooling and sealing cover 6, and perform cooling and grinding operations through the touch liquid crystal display screen 42. During the grinding process, liquid nitrogen in the liquid nitrogen tank 10 is injected into the vibration pulverizing box 5, and the vibration motor 15 is started to drive the support tray 8 and the test tube clamping seat 9 to perform pulverizing and grinding operations using the grinding beads 611. After grinding is completed, the entire test tube clamping seat 9 is transferred to the lysis chamber 12, and then placed on the test tube support bracket 112 in sequence and contacted with the detection probe group 111 one by one for analysis operations.

[0039] In the description of the present invention, it should be understood that the orientation or positional relationship indicated by the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", etc. is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation to the present invention.

[0040] Finally, it should be noted that the above are only the preferred embodiments of the present invention and are not used to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, for those skilled in the art, they can still modify the technical solutions recorded in the foregoing embodiments, or perform equivalent replacements for some of the technical features. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. An automated extraction device for wheat gene samples, characterized in that, The automatic extraction device for wheat gene samples includes an extraction and processing box (1), support feet (2), a sealing door (3), an automatic control panel group (4), a vibration and pulverization box (5), a cooling and sealing cover (6), an insulating handle (7), a coolant nitrogen tank (10), and an analysis and detection box (11). The support feet (2) are respectively bolted to the four lower corners of the extraction and processing box (1); the sealing door (3) is hinge-connected to the lower front side of the extraction and processing box (1); the automatic control panel group (4) is screwed to the upper front side of the extraction and processing box (1); the vibration and pulverization box (5) is screwed to the top of the extraction and processing box (1); the cooling and sealing cover (6) is snapped onto the upper end of the vibration and pulverization box (5) and is hinge-connected at the intersection of the rear part; the insulating handle (7) is screwed to the upper right side of the cooling and sealing cover (6); the coolant nitrogen tank (10) is arranged at the lower left inside of the extraction and processing box (1); the analysis and detection box (11) is bolted to the right inside position of the extraction and processing box (1).

2. The automated wheat gene sample extraction device according to claim 1, wherein, A vibration motor (15) is bolted to the lower left inside of the vibration and pulverization box (5); the output end of the vibration motor (15) is screwed with a support tray (8), and a test tube holder (9) is clamped on the upper part of the support tray (8).

3. The automated wheat gene sample extraction device according to claim 2, wherein Locking screws (81) are threadedly connected to both sides of the support tray (8). A plurality of restraint card holes (91) are formed in the upper part of the test tube holder (9). The test tube holder (9) is clamped in the support tray (8) and fixed with the locking screws (81).

4. The automated wheat gene sample extraction device according to claim 1, characterized in that A connecting pipe (13) is threadedly connected to the upper end of the analysis and detection box (11); a cracking chamber (12) is threadedly connected to the upper part of the connecting pipe (13); a processor (14) is welded to the left side of the analysis and detection box (11).

5. The automated wheat gene sample extraction device according to claim 1, characterized in that, A detection probe group (111) is inlaid on the left inside of the analysis and detection box (11). A test tube support bracket (112) is screwed to the right inside of the analysis and detection box (11). The test tube support bracket (112) and the detection probe group (111) are arranged in one-to-one correspondence.

6. The automated wheat gene sample extraction device according to claim 1, characterized in that, A storage box (61) is screwed to the lower left inside of the cooling and sealing cover (6). Grinding beads (611) are clamped in the storage box (61); the grinding beads (611) are ceramic grinding beads.

7. The automated wheat gene sample extraction device according to claim 1, characterized in that The described automated control panel group (4) includes an automated control panel main body (41), a touch liquid crystal display screen (42), an indicator light (43), function setting buttons (44), speed adjustment buttons (45), an emergency stop button (46), a confirmation start button (47), and a power button (48). The touch liquid crystal display screen (42) is embedded in the left side inside the automated control panel main body (41); the indicator light (43) is embedded in the left side of the automated control panel main body (41); the function setting buttons (44), speed adjustment buttons (45), and emergency stop button (46) are sequentially embedded in the upper right side of the automated control panel main body (41) from left to right; the confirmation start button (47) and the power button (48) are sequentially embedded in the lower right side of the automated control panel main body (41) from left to right.

8. The automated wheat gene sample extraction device according to claim 1, characterized in that, The described grinding beads (611) are ceramic grinding beads.

9. The automated wheat gene sample extraction device according to claim 1, wherein, The internal embedded bioinformatics processing unit of the described processor (14), real-time fluorescence quantitative PCR data analysis SNP marker automatic identification (applicable to wheat A / B / D genomes), and extraction quality dynamic feedback control system.

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