Methods, apparatus, devices, media, and products for repair of sequencing flow cells

Abstract

The application provides a method, device, equipment, medium and product for repairing a sequencing flow tank, and relates to the technical field of gene sequencing. The method comprises the following steps: determining the damage type of a target sequencing flow tank; if the damage type is a preset repairable type, determining a corresponding repair scheme based on the preset repairable type, wherein the repair scheme comprises cleaning treatment or cleaning treatment and repair treatment; and controlling a corresponding operation component to operate the target sequencing flow tank based on the repair scheme, so as to obtain a repaired sequencing flow tank. The method of the application realizes automatic repair of the sequencing flow tank, adopts a corresponding repair scheme for different types of sequencing flow tanks, so that all types of sequencing flow tanks can be accurately repaired, and the cleaning degree of the processed sequencing flow tank is greatly improved, thereby prolonging the service life of the sequencing flow tank while ensuring good use performance.

Description

Technical Field

[0001] The present application relates to the field of gene sequencing technology, and in particular to a repair method, device, equipment, medium and product for a sequencing flow cell. Background Art

[0002] The sequencing flow cell is the core component of the gene sequencing equipment. Oligonucleotide fragments complementary to the sequencing adapters are fixed on its surface for adsorption and amplification of DNA fragments.

[0003] To ensure the accuracy of sequencing results, sequencing flow cells are usually disposable consumables, but the cost of flow cells is relatively high, and if they are replaced immediately after use, it will cause a waste of resources.

[0004] In order to save usage costs, a series of cleaning, disinfection and repair methods are manually adopted, including ultrasonic cleaning, acid and alkali solution immersion, high temperature and high pressure sterilization, polishing and repair, etc., so that the sequencing flow cell can be reused.

[0005] However, existing methods usually rely on manual repair and lack automated means; incomplete cleaning may affect subsequent experimental results and cause cross-contamination, and unsatisfactory repair effects may affect the performance and service life of consumables. Summary of the Invention

[0006] The present application provides a repair method, device, equipment, medium and product for a sequencing flow cell to solve the technical problems of lack of automated means, incomplete cleaning and unsatisfactory repair effect.

[0007] In a first aspect, the present application provides a method for repairing a sequencing flow cell, the method comprising:

[0008] Determine the damage type of the target sequencing flow cell;

[0009] If the damage type is a preset repairable type, a corresponding repair scheme is determined based on the preset repairable type, and the repair scheme includes a cleaning process or a cleaning process and a repair process; wherein the cleaning process includes: cleaning the target sequencing flow cell with an enzymatic cleaning solution at a first preset temperature for a first preset time period, and then cleaning the target sequencing flow cell with an oxidizing cleaning solution until the residue concentration is less than a preset residue concentration threshold; the repair process includes: in response to the residue concentration being less than the preset residue concentration threshold, performing coating repair and / or hot pressing shaping on the target sequencing flow cell;

[0010] Based on the repair scheme, the corresponding operating component is controlled to operate the target sequencing flow cell to obtain a repaired sequencing flow cell.

[0011] In one possible design, the preset repairable type includes a first repairable type and a second repairable type, wherein the target sequencing flow cell of the first repairable type has no physical damage, and the target sequencing flow cell of the second repairable type has physical damage; and determining a corresponding repair solution based on the preset repairable type includes:

[0012] If the preset repairable type is the first repairable type, determining that the corresponding repair solution is a cleaning process;

[0013] If the preset repairable type is the second repairable type, the corresponding repair solution is determined to be a cleaning process and a repair process.

[0014] In one possible design, the repair scheme includes a cleaning process, and controlling a corresponding operating component to operate the target sequencing flow cell based on the repair scheme includes:

[0015] Controlling the temperature control component to set the temperature to a first preset temperature;

[0016] In response to reaching the first preset temperature, controlling the grabbing component to place the target sequencing flow cell in a cleaning container of the enzymatic cleaning solution, and using the enzymatic cleaning solution to clean the target sequencing flow cell;

[0017] In response to determining that the target sequencing flow cell has been cleaned with the enzymatic cleaning solution for a first preset time, the grabbing component is controlled to move the target sequencing flow cell into a cleaning container of the oxidative cleaning solution, and the ultrasonic component is controlled to turn on so that the target sequencing flow cell is cleaned by the oxidative cleaning solution under an ultrasonic environment.

[0018] In one possible design, the cleaning process further includes determining a residue concentration of the target sequencing flow cell, and controlling a corresponding operating component to operate the target sequencing flow cell based on the repair scheme includes:

[0019] Controlling an online ultraviolet absorbance detector and a fluorescence detector to detect the surface of the target sequencing flow cell;

[0020] receiving first detection results sent by the online ultraviolet absorption detector and the fluorescence detector;

[0021] Determine the residue concentration of the target sequencing flow cell based on the first detection result.

[0022] In one possible design, the repair scheme includes a repair process, and controlling a corresponding operating component to operate the target sequencing flow cell based on the repair scheme includes:

[0023] Controlling the grabbing component to place the target sequencing flow cell in a plasma surface activation processor for activation treatment;

[0024] In response to the activation treatment duration reaching a second preset duration, controlling the spraying component to spray the repair liquid on the surface of the target sequencing flow cell;

[0025] And / or, control the grabbing component to place the target sequencing flow cell in a preset mold, and control the temperature to be a second preset temperature and the pressure to be a preset pressure value to achieve hot pressing shaping of the target sequencing flow cell.

[0026] In one possible design, before determining the damage type of the target sequencing flow cell, the method further includes:

[0027] Controlling a computer vision sensor to capture an image of the target sequencing flow cell;

[0028] Determining the damage type of the target sequencing flow cell includes:

[0029] receiving an image sent by the computer vision sensor, wherein the image includes a target sequencing flow cell;

[0030] A damage type of the target sequencing flow cell is determined based on the image.

[0031] In one possible design, determining the damage type of the target sequencing flow cell based on the image includes:

[0032] Determining the damage type of the target sequencing flow cell based on the image using a preset damage type determination model;

[0033] If the image includes surface scratches and / or structural deformation, determining that the damage type of the target sequencing flow cell is a second repairable type;

[0034] If the image does not include surface scratches and / or structural deformation, it is determined that the damage type of the target sequencing flow cell is a first repairable type.

[0035] In a possible design, the step of training the preset damage type determination model includes:

[0036] Acquire a training sample set, the training sample set including image samples of a plurality of sequencing flow cells, the image samples of the plurality of sequencing flow cells including samples including surface scratches and / or structural deformations in the images and samples not including surface scratches and structural deformations in the images;

[0037] Using the training sample set to train the initial damage type determination model;

[0038] In response to the model convergence condition being satisfied, the initial damage type determination model satisfying the model convergence condition is determined as the preset damage type determination model.

[0039] In one possible design, the first preset temperature is 35°C to 45°C;

[0040] and / or, the enzymatic cleaning solution comprises proteinase K and sodium lauryl sulfate;

[0041] And / or, the oxidizing cleaning solution includes peroxide water and citric acid.

[0042] In one possible design, before determining the damage type of the target sequencing flow cell, the method further includes:

[0043] The cleaning component is controlled to remove the sealing film and sealant residues on the surface of the target sequencing flow cell.

[0044] In one possible design, after controlling the corresponding operating component based on the repair scheme to repair the target sequencing flow cell to obtain a repaired sequencing flow cell, the method further includes:

[0045] Controlling an optical detector and a sequencing verifier to detect the repaired sequencing flow cell;

[0046] receiving a second detection result sent by the optical detector and the sequencing verification instrument;

[0047] Determining whether the repair is successful according to the second detection result;

[0048] The detection indicators include at least two of the following:

[0049] Flatness, residue concentration, transmittance, surface roughness, output data volume, and base error rate.

[0050] In a second aspect, the present application provides a repair device for a sequencing flow cell, the device comprising:

[0051] a determination module, for determining a damage type of a target sequencing flow cell;

[0052] The determination module is further configured to, if the damage type is a preset repairable type, determine a corresponding repair scheme based on the preset repairable type, the repair scheme including a cleaning process or a cleaning process and a repair process; wherein the cleaning process includes: cleaning the target sequencing flow cell with an enzymatic cleaning solution at a first preset temperature for a first preset time period, and then cleaning the target sequencing flow cell with an oxidizing cleaning solution until the residue concentration is less than a preset residue concentration threshold; and the repair process includes: in response to the residue concentration being less than the preset residue concentration threshold, performing coating repair and / or hot pressing shaping on the target sequencing flow cell;

[0053] A control module is used to control the corresponding operating component to operate the target sequencing flow cell based on the repair scheme to obtain a repaired sequencing flow cell.

[0054] In a third aspect, the present application provides a repair device for a sequencing flow cell, the device comprising: a processor, and a memory communicatively connected to the processor;

[0055] The memory is used to store computer-executable instructions;

[0056] The processor is used to execute the computer-executable instructions stored in the memory to implement the method as described in any one of the first aspects.

[0057] In a fourth aspect, the present application provides a computer-readable storage medium, wherein the computer-readable storage medium stores computer-executable instructions, and when the computer-executable instructions are executed by a processor, they are used to implement the method as described in any one of the first aspects.

[0058] In a fifth aspect, the present application provides a computer program product, comprising a computer program, which, when executed by a processor, implements the method as described in any one of the first aspects.

[0059] The repair method, apparatus, equipment, medium and product for a sequencing flow cell provided in the present application determine the damage type of a target sequencing flow cell; if the damage type is a preset repairable type, a corresponding repair scheme is determined based on the preset repairable type, and the repair scheme includes a cleaning process or a cleaning process and a repair process; wherein the cleaning process includes: at a first preset temperature, after cleaning the target sequencing flow cell with an enzymatic cleaning solution for a first preset time, cleaning the target sequencing flow cell with an oxidizing cleaning solution until the residue concentration is less than a preset residue concentration threshold; the repair process includes: in response to the residue concentration being less than the preset residue concentration threshold, performing coating repair and / or hot pressing shaping on the target sequencing flow cell; and controlling the corresponding operating component to operate the target sequencing flow cell based on the repair scheme to obtain a repaired sequencing flow cell. By determining the damage type of the target sequencing flow cell, the most appropriate repair measures can be taken for different damage situations, avoiding the waste of resources caused by blind repair and possible secondary damage to the flow cell, and improving the repair efficiency and success rate; the preset repairable types and their corresponding repair plans make the repair process standardized and streamlined; in the cleaning process, first use enzymatic cleaning solution to clean at a specific temperature, which can effectively decompose and remove organic residues on the surface of the target sequencing flow cell, and then use oxidative cleaning solution for further cleaning until the residue concentration is lower than the preset threshold, ensuring the thorough cleaning of the flow cell. The two-step cleaning strategy combines the advantages of enzymatic hydrolysis and oxidation, improves the cleaning efficiency and effect, and reduces the sequencing residues caused by residues. sequencing error; after the residue concentration meets the requirements, repair treatment is carried out. The scheme provides the options of coating repair and / or hot pressing shaping to deal with different types of physical damage. Coating repair can restore the surface performance of the flow cell, while hot pressing shaping can correct structural problems caused by physical deformation, thereby improving the repair utilization rate and life of the flow cell; based on the repair scheme, the corresponding operating components are controlled to operate the target sequencing flow cell, realizing the automation and intelligence of the repair process, which not only reduces manual intervention and reduces operational errors, but also improves repair efficiency and consistency; through the above series of precise and efficient repair measures, the repaired sequencing flow cell can significantly improve the accuracy and reliability of sequencing and achieve recycling and reuse. BRIEF DESCRIPTION OF THE DRAWINGS

[0060] The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments consistent with the present application and, together with the description, serve to explain the principles of the present application.

[0061] Figure 1 A diagram illustrating an application scenario of a repair method for a sequencing flow cell provided in one embodiment of the present application;

[0062] Figure 2A flowchart of a method for repairing a sequencing flow cell provided in one embodiment of the present application;

[0063] Figure 3 A flowchart of a method for repairing a sequencing flow cell provided in another embodiment of the present application;

[0064] Figure 4 A schematic diagram of the structure of a repair device for a sequencing flow cell provided in one embodiment of the present application;

[0065] Figure 5 A schematic diagram of the structure of a repair device for a sequencing flow cell provided in one embodiment of the present application.

[0066] The above drawings illustrate specific embodiments of the present application, which will be described in more detail below. These drawings and the textual description are not intended to limit the scope of the present application in any way, but rather to illustrate the concepts of the present application to those skilled in the art by reference to specific embodiments. DETAILED DESCRIPTION

[0067] Exemplary embodiments will be described in detail herein, with examples illustrated in the accompanying drawings. In the following description, when referring to the drawings, identical numerals in different figures represent identical or similar elements, unless otherwise indicated. The embodiments described in the following exemplary embodiments are not intended to represent all embodiments consistent with the present application. Rather, they are merely examples of apparatus and methods consistent with certain aspects of the present application, as detailed in the appended claims.

[0068] It should be noted that in the embodiments of the present application, certain software, components, and models may be mentioned, which should be regarded as exemplary. Their purpose is only to illustrate the feasibility of implementing the technical solution of the present application, but it does not mean that the applicant has or will necessarily use the solution.

[0069] In order to clearly understand the technical solution of the present application, the solution of the prior art is first introduced in detail.

[0070] In the field of gene sequencing, the sequencing flow cell is the core component of the gene sequencing equipment. Oligonucleotide fragments complementary to the sequencing adapters are fixed on its surface, which are used to adsorb and amplify DNA fragments. Its performance directly affects the sequencing quality and efficiency. With the rapid development of high-throughput sequencing technology, the requirements for the accuracy and stability of the flow cell are increasing. However, during the use of the flow cell, physical damage, chemical residues or impurity accumulation often cause the sequencing signal to attenuate and the error rate to increase, affecting the sequencing accuracy. Existing repair solutions mostly focus on manually adopting a series of cleaning, disinfection and repair methods, including ultrasonic cleaning, acid and alkali solution immersion, high temperature and high pressure sterilization, polishing and repair, etc., but these methods are often difficult to completely remove stubborn residues, and may cause secondary damage to the surface of the flow cell, reducing its service life. In addition, there is a lack of standardized automated processes, making it difficult to ensure the consistency and reliability of the repair effect.

[0071] Therefore, when facing the technical problems in the existing technology, in order to improve the accuracy of the repair measures, the damage type of the target sequencing flow cell is first determined, and the corresponding repair scheme is determined according to the different damage types. This not only improves the repair efficiency, but also presets the repairable types and their corresponding repair schemes, so that the repair process is standardized and streamlined; to improve the cleaning effect, an enzymatic cleaning solution and an oxidative cleaning solution are used for two-step cleaning, which combines the advantages of enzymatic hydrolysis and oxidation, improves the cleaning efficiency and effect, and reduces sequencing errors caused by residues; since the sequencing flow cell may also have different degrees of physical damage, different repair treatments are set up, including coating repair and / or hot pressing shaping, to adapt to various damage types, thereby improving the repair utilization rate and life of the flow cell; to reduce manual intervention and reduce operational errors, the corresponding operating components are controlled based on the repair scheme to operate the target sequencing flow cell, thereby realizing the automation and intelligence of the repair process, ensuring the consistency and reliability of the repair effect, and realizing recycling and reuse.

[0072] Figure 1 This is an application scenario diagram of the repair method of the sequencing flow cell provided in one embodiment of the present application, such as Figure 1 As shown, the application scenario diagram of the sequencing flow cell repair method provided in this embodiment includes: a server 101 and an operating device 102. It can be understood that the server 101 is integrated with a sequencing flow cell repair device for executing the sequencing flow cell repair method provided in this application; the operating device 102 includes all operating components required for the repair solution; and the server 101 is in communication with the operating device 102.

[0073] Optionally, the repair device of the sequencing flow cell can also be integrated into a device including all operating components, which is not limited in this embodiment.

[0074] Specifically, the server 101 first determines the damage type of the target sequencing flow cell. If the damage type is a preset repairable type, the corresponding repair plan is determined based on the preset repairable type. The repair plan includes cleaning treatment or cleaning treatment and repair treatment; then the server 101 controls the corresponding operating component in the operating device 102 to operate the target sequencing flow cell based on the repair plan to obtain a repaired sequencing flow cell.

[0075] Optionally, the server 101 may send a control instruction to the operating device 102 so that the operating device 102 controls the corresponding operating component to operate after receiving the control instruction. Alternatively, the server 101 may control the corresponding operating component to operate in other ways, which is not limited in this embodiment.

[0076] Optionally, the operating device 102 can be used to collect data on the target sequencing flow cell and send the relevant data to the server 101, so that the server 101 determines the damage type of the target sequencing flow cell based on the relevant data; or other methods can be used to determine the damage type of the target sequencing flow cell, which is not limited in this embodiment.

[0077] The following specific embodiments describe in detail the technical solution of the present application and how the technical solution of the present application solves the above-mentioned technical problems. The following specific embodiments can be combined with each other, and the same or similar concepts or processes may not be repeated in some embodiments. The embodiments of the present application will be described below in conjunction with the accompanying drawings.

[0078] Figure 2 A flowchart of a repair method for a sequencing flow cell provided in one embodiment of the present application is shown in FIG. Figure 2 As shown, the execution subject of this embodiment is a repair device for a sequencing flow cell. The repair device for a sequencing flow cell can be implemented by a computer program, or by a medium storing a relevant computer program, such as a USB flash drive and / or an optical disk, or can be integrated into a repair device for a sequencing flow cell, such as a server. The repair method for a sequencing flow cell provided in this embodiment includes the following steps:

[0079] Step 201: Determine the damage type of the target sequencing flow cell.

[0080] The target sequencing flow cell refers to the sequencing flow cell that needs to be repaired.

[0081] It is understandable that the sequencing flow cell may suffer various damages during use, such as contamination, scratches, deformation, etc.

[0082] Exemplarily, an image of the target sequencing flow cell sent by an image acquisition device can be received, such as an image of each surface of the target sequencing flow cell, and the received image of the target sequencing flow cell can be compared with a reference sequencing flow cell image pre-stored in a repair device of the sequencing flow cell to determine the damage type of the target sequencing flow cell.

[0083] The benchmark sequencing flow cell image refers to an image of a brand new, undamaged sequencing flow cell.

[0084] Optionally, the damage type of the target sequencing flow cell can be determined by calculating the pixel difference vector between the reference sequencing flow cell image and the target sequencing flow cell image, and then comparing it with a preset difference threshold. Other comparison methods may also be used, and this embodiment does not limit this.

[0085] Optionally, a pre-trained model may be used to determine the damage type of the target sequencing flow cell based on the image of the target sequencing flow cell, which is not limited in this embodiment.

[0086] It is understandable that, in order to improve the accuracy of determining the damage type, the sealing film and sealant residues on the surface of the target sequencing flow cell may be removed before determining the damage type of the target sequencing flow cell.

[0087] Optionally, before determining the damage type of the target sequencing flow cell, the target sequencing flow cell can be identified by controlling the spectral sensor, such as identifying the code on the surface of the target sequencing flow cell or judging the material and other characteristics of the flow cell based on the laser reflection characteristics, and using this as a label of the target sequencing flow cell for identification and distinction, or distinguishing the target sequencing flow cell by other means, which is not limited in this embodiment.

[0088] In step 202, if the damage type is a preset repairable type, a corresponding repair scheme is determined based on the preset repairable type, and the repair scheme includes a cleaning process or a cleaning process and a repair process; wherein the cleaning process includes: at a first preset temperature, using an enzymatic cleaning solution to clean the target sequencing flow cell for a first preset time period, and then using an oxidizing cleaning solution to clean the target sequencing flow cell until the residue concentration is less than a preset residue concentration threshold; the repair process includes: in response to the residue concentration being less than the preset residue concentration threshold, performing coating repair and / or hot pressing shaping on the target sequencing flow cell.

[0089] Among them, the preset repairable type refers to the type name that can be repaired, such as the first type, the second type, etc., which is pre-configured in the repair device of the sequencing flow cell.

[0090] Among them, the first preset temperature refers to the temperature during the cleaning process, which is pre-configured in the repair device of the sequencing flow cell, such as 40°C, 45°C, etc., and this embodiment does not limit this.

[0091] Among them, the first preset time refers to the time for cleaning with enzymatic cleaning solution, which is pre-configured in the repair device of the sequencing flow tank, such as 10 minutes, 20 minutes, etc., and this embodiment does not limit this.

[0092] The preset residue concentration threshold refers to a value preset in the repair device of the sequencing flow cell for determining whether the residue concentration on the surface of the target sequencing flow cell meets the expected value, such as 0.1 ng / mm², etc. This embodiment does not limit this.

[0093] Among them, enzymatic cleaning liquid refers to a liquid containing specific enzymes used to catalyze hydrolysis reactions of proteins, polysaccharides, etc.

[0094] Among them, the oxidizing cleaning liquid refers to a liquid containing a certain oxidant and used to decompose trace elements.

[0095] Specifically, after determining the damage type of the target sequencing flow cell, the type is compared with the preset repairable type. If the damage type is the preset repairable type, the specific repair solution can be determined based on the mapping relationship between the repairable type and the repair solution preset in the repair device of the sequencing flow cell.

[0096] Exemplarily, if it is determined that the damage type of the target sequencing flow cell is the first type, and the preset repairable type includes the first type and the second type, then the damage type of the target sequencing flow cell is determined to be the preset repairable type. Since the repair device of the sequencing flow cell is pre-configured with a mapping relationship between the repairable type and the repair scheme, such as the first type corresponds to a cleaning process, and the second type corresponds to a cleaning process plus a repair process, then according to the mapping relationship, it is determined that the repair scheme corresponding to the target sequencing flow cell is the cleaning process.

[0097] It is understandable that after determining the damage type of the target sequencing flow cell, there may still be an irreparable type, such as the third type. This type is not among the preset repairable types, and the target sequencing flow cell cannot be repaired. Other treatments can be performed on it, such as disassembling the sequencing flow cell and extracting raw materials, or discarding it, etc. This embodiment does not limit this.

[0098] Optionally, during the cleaning process, the residue concentration on the surface of the target sequencing flow cell can be detected in real time by fluorescence detection or other measures until the residue concentration is less than a preset residue concentration threshold; or detection can be performed by other means, which is not limited in this embodiment.

[0099] Step 203: Based on the repair solution, the corresponding operating component is controlled to operate the target sequencing flow cell to obtain a repaired sequencing flow cell.

[0100] Exemplarily, the repair scheme includes cleaning and repair treatments. The target sequencing flow cell can be placed on a conveyor belt when the damage type is determined. After the damage type is determined, the conveyor belt is controlled to move it to a designated position, such as first moving it to the cleaning treatment position, controlling the temperature control component to set the temperature to a first preset temperature, and then controlling the nozzle to start spraying an enzymatic cleaning solution, so that the target sequencing flow cell can be cleaned by the enzymatic cleaning solution at the first preset temperature until a first preset time is reached. The nozzle is controlled to stop spraying the enzymatic cleaning solution and start spraying the oxidizing cleaning solution, so that the target sequencing flow cell can be cleaned by the oxidizing cleaning solution at the first preset temperature until the residue concentration is less than a preset residue concentration threshold; then the conveyor belt is controlled to move the cleaned target sequencing flow cell to the repair treatment position, and the spraying component is controlled to spray the repair solution on the surface of the target sequencing flow cell and / or the shaping component is controlled to perform hot pressing on the target sequencing flow cell according to the repair scheme.

[0101] Optionally, the nozzles for spraying the enzymatic cleaning solution and the oxidative cleaning solution may be the same or different, which is not limited in this embodiment.

[0102] It should be noted that the two cleaning liquids cannot be mixed. That is, if the same nozzle is used for spraying, the two cleaning liquids must be placed in different containers to avoid mixing and interfering with the cleaning effect.

[0103] Optionally, the target sequencing flow cell may be moved to a specified position by controlling other components such as a robotic arm, which is not limited in this embodiment.

[0104] Optionally, each target sequencing flow cell may be processed individually, or multiple target sequencing flow cells may be processed in batches simultaneously, which is not limited in this embodiment.

[0105] It is understandable that the above descriptions are all exemplary descriptions, and specific operations may also be performed by controlling other specific operating components, which is not limited in this embodiment.

[0106] The repair method of a sequencing flow cell provided in an embodiment of the present application determines the damage type of a target sequencing flow cell; if the damage type is a preset repairable type, a corresponding repair scheme is determined based on the preset repairable type, and the repair scheme includes a cleaning process or a cleaning process and a repair process; wherein the cleaning process includes: at a first preset temperature, after cleaning the target sequencing flow cell with an enzymatic cleaning solution for a first preset time, cleaning the target sequencing flow cell with an oxidizing cleaning solution until the residue concentration is less than a preset residue concentration threshold; the repair process includes: in response to the residue concentration being less than the preset residue concentration threshold, performing coating repair and / or hot pressing shaping on the target sequencing flow cell; based on the repair scheme, controlling the corresponding operating component to operate the target sequencing flow cell to obtain a repaired sequencing flow cell. By determining the damage type of the target sequencing flow cell, the most appropriate repair measures can be taken for different damage situations, avoiding the waste of resources caused by blind repair and possible secondary damage to the flow cell, and improving the repair efficiency and success rate; the preset repairable types and their corresponding repair plans make the repair process standardized and streamlined; in the cleaning process, first use enzymatic cleaning solution to clean at a specific temperature, which can effectively decompose and remove organic residues on the surface of the target sequencing flow cell, and then use oxidative cleaning solution for further cleaning until the residue concentration is lower than the preset threshold, ensuring the thorough cleaning of the flow cell. The two-step cleaning strategy combines the advantages of enzymatic hydrolysis and oxidation, improves the cleaning efficiency and effect, and reduces the sequencing residues caused by residues. sequencing error; after the residue concentration meets the requirements, repair treatment is carried out. The scheme provides the options of coating repair and / or hot pressing shaping to deal with different types of physical damage. Coating repair can restore the surface performance of the flow cell, while hot pressing shaping can correct structural problems caused by physical deformation, thereby improving the repair utilization rate and life of the flow cell; based on the repair scheme, the corresponding operating components are controlled to operate the target sequencing flow cell, realizing the automation and intelligence of the repair process, which not only reduces manual intervention and reduces operational errors, but also improves repair efficiency and consistency; through the above series of precise and efficient repair measures, the repaired sequencing flow cell can significantly improve the accuracy and reliability of sequencing and achieve recycling and reuse.

[0107] As an optional implementation, based on the above embodiment, the preset repairable type includes a first repairable type and a second repairable type, wherein the target sequencing flow cell of the first repairable type has no physical damage, and the target sequencing flow cell of the second repairable type has physical damage; determining a corresponding repair solution based on the preset repairable type includes:

[0108] If the preset repairable type is the first repairable type, determining the corresponding repair solution as cleaning;

[0109] If the preset repairable type is the second repairable type, the corresponding repair solution is determined to be a cleaning process and a repair process.

[0110] Among them, physical damage may include surface scratches, structural deformation, etc.

[0111] It is understandable that the surface of the sequencing flow cell will be contaminated after use, such as by the presence of proteins, fluorescent dyes, metal ions, etc.

[0112] Specifically, if the preset repairable type is the first repairable type, that is, there is no physical damage to the target sequencing flow cell and only surface contamination exists, then the repair solution is determined to be cleaning treatment, and the target sequencing flow cell is cleaned to remove surface contamination; if the preset repairable type is the second repairable type, that is, the target sequencing flow cell not only has surface contamination but also physical damage, then the repair solution is determined to be cleaning treatment and repair treatment, and the target sequencing flow cell is first cleaned to remove surface contamination, and then repair treatment is performed to improve physical damage.

[0113] The repair method of the sequencing flow cell provided in the embodiment of the present application, the preset repairable type includes a first repairable type and a second repairable type, wherein the target sequencing flow cell of the first repairable type does not have physical damage, and the target sequencing flow cell of the second repairable type has physical damage; based on the preset repairable type, the corresponding repair scheme is determined, including: if the preset repairable type is the first repairable type, then the corresponding repair scheme is determined to be a cleaning process; if the preset repairable type is the second repairable type, then the corresponding repair scheme is determined to be a cleaning process and a repair process. Differentiated repair schemes are adopted for different repairable types, which can accurately respond to the damage condition of the target sequencing flow cell. For the first repairable type, only a cleaning process is performed to restore its normal sequencing function, avoiding unnecessary damage caused by excessive repair; for the second repairable type, a repair process is added on the basis of the cleaning process, which can specifically repair the physical damage and restore the flow cell to a state close to its original state, ensuring the accuracy and reliability of sequencing; the precise repair scheme avoids secondary damage to the sequencing flow cell caused by inappropriate repair operations and improves the repair efficiency of the sequencing flow cell.

[0114] As an optional implementation, based on the above embodiment, the repair solution includes a cleaning process, and controlling the corresponding operating components to operate the target sequencing flow cell based on the repair solution includes:

[0115] Controlling the temperature control component to set the temperature to a first preset temperature;

[0116] In response to reaching the first preset temperature, controlling the grabbing component to place the target sequencing flow cell in a cleaning container of an enzymatic cleaning solution, and using the enzymatic cleaning solution to clean the target sequencing flow cell;

[0117] In response to determining that the target sequencing flow cell has been cleaned with the enzymatic cleaning solution for a first preset time, the grabbing component is controlled to move the target sequencing flow cell into a cleaning container of the oxidizing cleaning solution, and the ultrasonic component is controlled to turn on so that the target sequencing flow cell is cleaned with the oxidizing cleaning solution under an ultrasonic environment.

[0118] The temperature control component refers to a component used to monitor, control and adjust the temperature, such as a temperature controller, etc., which is not limited in this embodiment.

[0119] The grasping component refers to a component used to grasp an object and move the object to a specified position, such as a robotic arm, etc., which is not limited in this embodiment.

[0120] The ultrasonic component refers to a component for emitting ultrasonic waves, such as an ultrasonic generator, etc., which is not limited in this embodiment.

[0121] The ultrasonic vibration frequency may be pre-configured in the repair device of the sequencing flow cell to control the ultrasonic component to vibrate at the frequency, such as 40 kHz, which is not limited in this embodiment.

[0122] It can be understood that the ultrasonic component emits ultrasonic waves to the cleaning container of the oxidative cleaning solution so that the target sequencing flow cell is cleaned under the vibration of the ultrasonic wave.

[0123] Specifically, when performing the cleaning process, the temperature control component is first controlled to set the temperature to a first preset temperature so that the target sequencing flow cell can be cleaned at the first preset temperature. When the temperature reaches the first preset temperature, the grabbing component is controlled to move the target sequencing flow cell to a cleaning container of an enzymatic cleaning solution, and the container containing the enzymatic cleaning solution is controlled to start flowing out the enzymatic cleaning solution, such as using a nozzle to spray the cleaning solution, so that the target sequencing flow cell is rinsed with the enzymatic cleaning solution in the cleaning container; when the time for cleaning the target sequencing flow cell with the enzymatic cleaning solution reaches a first preset time, the container containing the enzymatic cleaning solution is controlled to stop flowing out the enzymatic cleaning solution, and the grabbing component is controlled to move the target sequencing flow cell to a cleaning container of an oxidizing cleaning solution, and at the same time, the ultrasonic component is controlled to start at a preset vibration frequency and the container containing the oxidizing cleaning solution starts flowing out the oxidizing cleaning solution, so that the target sequencing flow cell is cleaned by the oxidizing cleaning solution under an ultrasonic environment.

[0124] Optionally, during the cleaning process, the real-time detection component can be controlled to detect the residue concentration of the liquid in the cleaning container, such as detecting the content of DNA and protein by ultraviolet light, or detecting the signal intensity of the fluorescent dye at the wavelength of Ex / Em=488 / 520 nm, etc., until the residue concentration is less than a preset residue concentration threshold. This embodiment does not limit this.

[0125] The repair method of the sequencing flow cell provided in the embodiment of the present application is to use an enzymatic cleaning solution to clean the target sequencing flow cell for a first preset time at a first preset temperature, and then use an oxidizing cleaning solution to clean the target sequencing flow cell, including: controlling the temperature control component to set the temperature to the first preset temperature; in response to reaching the first preset temperature, controlling the grabbing component to place the target sequencing flow cell in a cleaning container of the enzymatic cleaning solution, and using the enzymatic cleaning solution to clean the target sequencing flow cell; in response to determining that the target sequencing flow cell has been cleaned for a first preset time using the enzymatic cleaning solution, controlling the grabbing component to move the target sequencing flow cell to the cleaning container of the oxidizing cleaning solution, and controlling the ultrasonic component to turn on so that the target sequencing flow cell is cleaned by the oxidizing cleaning solution under an ultrasonic environment. By controlling the temperature control component, the grabbing component, etc. to perform operations, an automated process is realized, the repair work of the sequencing flow cell is standardized, and the efficiency and reliability of the repair are improved; when performing oxidizing cleaning, turning on the ultrasonic component accelerates the shedding of impurities attached to the surface of the sequencing flow cell and in tiny gaps, thereby improving the cleaning effect.

[0126] As an optional embodiment, based on the above embodiment, the cleaning process further includes determining the residue concentration of the target sequencing flow cell, and controlling the corresponding operating component to operate the target sequencing flow cell based on the repair plan, including:

[0127] Control the online UV absorbance detector and fluorescence detector to detect the surface of the target sequencing flow cell;

[0128] Receiving a first detection result sent by an online ultraviolet absorption detector and a fluorescence detector;

[0129] The residue concentration of the target sequencing flow cell is determined based on the first detection result.

[0130] Among them, the first detection result refers to the data sent by the online ultraviolet absorbance detector and fluorescence detector, which includes relevant data for calculating the residue concentration.

[0131] Specifically, after using the oxidizing cleaning solution for cleaning for a certain period of time, the extraction component is controlled to extract the residue sample on the surface of the target sequencing flow cell, and place it in the online ultraviolet absorbance detector and fluorescence detector for detection. After the online ultraviolet absorbance detector and fluorescence detector complete the detection, the detection results are sent to the sequencing flow cell repair device. After receiving the first detection result, the sequencing flow cell repair device calculates the residue concentration of the target sequencing flow cell according to a pre-configured calculation formula.

[0132] Optionally, an online ultraviolet absorption detector and a fluorescence detector may be controlled to perform imaging detection on the surface of the target sequencing flow cell, which is not limited in this embodiment.

[0133] Optionally, if the residue concentration is greater than a preset residue concentration threshold, the cleaning may be repeated until the residue concentration is less than the preset residue concentration threshold.

[0134] The repair method for a sequencing flow cell provided in an embodiment of the present application further includes the following steps: controlling an online ultraviolet absorbance detector and a fluorescence detector to detect the surface of the target sequencing flow cell; receiving a first detection result sent by the online ultraviolet absorbance detector and the fluorescence detector; and determining the residue concentration of the target sequencing flow cell based on the first detection result. The online ultraviolet absorbance detector and the fluorescence detector can accurately detect the surface of the target sequencing flow cell in real time and accurately quantify the residue concentration, which helps determine whether the cleaning has achieved the desired effect and avoids the impact of excessive residue on the accuracy and reliability of subsequent sequencing. Automated and intelligent control not only improves cleaning efficiency but also reduces the impact of human factors on cleaning quality, making the cleaning process more stable and reliable.

[0135] As an optional implementation, based on the above embodiment, the repair solution includes a repair process, and controlling the corresponding operating component to operate the target sequencing flow cell based on the repair solution, including:

[0136] Controlling the grabbing component to place the target sequencing flow cell in a plasma surface activation processor for activation treatment;

[0137] In response to the activation treatment duration reaching a second preset duration, controlling the spraying component to spray the repair liquid on the surface of the target sequencing flow cell;

[0138] And / or, control the grabbing component to place the target sequencing flow cell in a preset mold, and control the temperature to a second preset temperature and the pressure to a preset pressure value to achieve hot pressing shaping of the target sequencing flow cell.

[0139] Among them, the grabbing component refers to a component used to grab the target sequencing flow cell and move it to a specified position, such as a vacuum suction cup, parallel air grippers, etc., which is not limited in this embodiment.

[0140] The second preset duration refers to the duration of the activation treatment, which is pre-configured in the repair device of the sequencing flow cell, such as 20s, 30s, etc., and this embodiment does not limit this.

[0141] Among them, the preset mold is a mold used for hot pressing and shaping the target sequencing flow cell, and its size is consistent with that of the standard sequencing flow cell so that the target sequencing flow cell can be restored to its original shape.

[0142] Among them, the second preset temperature is the temperature of hot pressing and shaping, which is pre-configured in the repair device of the sequencing flow cell, such as 80°C, 90°C, etc., and this embodiment does not limit this.

[0143] The preset pressure value refers to the pressure value applied during hot pressing and shaping, which is pre-configured in the repair device of the sequencing flow cell, such as 10 kPa, 20 kPa, etc., and is not limited in this embodiment.

[0144] Specifically, after the cleaning process is completed, if coating repair is required, the grabbing component is controlled to move the target sequencing flow cell into the plasma surface activation processor and the plasma surface activation processor is controlled to start running according to pre-configured parameters such as activation power to activate the target sequencing flow cell. When the activation treatment time reaches the second preset time, the grabbing component is controlled to remove the activated target flow cell from the plasma surface activation processor, and placed in the spraying position to start controlling the spraying component to spray the repair liquid on the surface of the target sequencing flow cell so that the scratches on the target sequencing flow cell are repaired. If hot pressing shaping is required, the grabbing component is controlled to move the target sequencing flow cell into a preset mold, and the temperature and pressure of the preset mold are controlled according to the second preset temperature and preset pressure values, so that the target sequencing flow cell can be hot pressed under these conditions.

[0145] The activation process may be performed in a Yaqi atmosphere, and the activation power may be 50W, which is not limited in this embodiment.

[0146] The repair liquid may be a silicon-based nano-repair liquid, and the spraying thickness may be set to be ≤200 nm, which is not limited in this embodiment.

[0147] The processing time of hot pressing and shaping can be 5 minutes, and the geometric dimension error can be controlled within 0.1 μm / mm, which is not limited in this embodiment.

[0148] The repair method for a sequencing flow cell provided in an embodiment of the present application performs coating repair and / or hot pressing shaping on a target sequencing flow cell, comprising: controlling a grabbing component to place the target sequencing flow cell in a plasma surface activation processor for activation treatment; in response to the duration of the activation treatment reaching a second preset duration, controlling a spraying component to spray a repair liquid on the surface of the target sequencing flow cell; and / or controlling a grabbing component to place the target sequencing flow cell in a preset mold, and controlling the temperature to be a second preset temperature and the pressure to be a preset pressure value, so as to achieve hot pressing shaping of the target sequencing flow cell. Activating the target sequencing flow cell can form a large number of active groups on the surface, thereby enhancing the adhesion between the coating and the surface of the sequencing flow cell; controlling the spray assembly to spray the repair liquid on the surface of the target sequencing flow cell after the activation treatment can accurately control the spraying flow, speed and angle according to actual needs, further ensuring the uniformity of the coating, so that the surface of the target sequencing flow cell can restore the optical flatness and hydrophilic properties; placing the target sequencing flow cell in a preset mold, and controlling the temperature to a second preset temperature and the pressure to a preset pressure value for hot pressing and shaping. The shape of the mold can accurately determine the final shape of the flow cell, thereby achieving precise repair of the shape of the sequencing flow cell and ensuring platform compatibility.

[0149] As an optional implementation, based on the above embodiment, before determining the damage type of the target sequencing flow cell, the method further includes:

[0150] Control the computer vision sensor to capture images of the target sequencing flow cell;

[0151] Determine the type of damage to the sequencing flow cell of interest, including:

[0152] receiving an image sent by a computer vision sensor, wherein the image includes a target sequencing flow cell;

[0153] Determine the damage type of the target sequencing flow cell based on the image.

[0154] The images sent by the computer vision sensor may include images of various surfaces of the target sequencing flow cell.

[0155] Optionally, the computer vision sensor may be a video camera, a still camera, etc., which is not limited in this embodiment.

[0156] Specifically, before determining the damage type of the target sequencing flow cell, the computer vision sensor is first controlled to capture images of the target sequencing flow cell, such as capturing images of each surface of the target sequencing flow cell, and then packaging the images and sending them to a repair device for the sequencing flow cell. After the repair device for the sequencing flow cell receives the images sent by the computer vision sensor, it determines the damage type of the target sequencing flow cell based on the images.

[0157] For example, an artificial intelligence image recognition system can be pre-configured in the repair device of the sequencing flow cell to determine the damage type of the target sequencing flow cell based on the image; or the received image can be compared with the original sequencing flow cell image to determine the damage type. This embodiment does not limit this.

[0158] The sequencing flow cell repair method provided in an embodiment of the present application determines the damage type of a target sequencing flow cell, comprising: controlling a computer vision sensor to capture an image of the target sequencing flow cell; receiving an image sent by the computer vision sensor, the image including the target sequencing flow cell; and determining the damage type of the target sequencing flow cell based on the image. The computer vision sensor can capture images of the target sequencing flow cell at extremely high speeds, improving classification efficiency compared to manual inspection. The image has high resolution, capable of capturing subtle damage on the surface of the target sequencing flow cell, ensuring accuracy when determining the damage type of the target sequencing flow cell based on the image. Combining the computer vision sensor with a sequencing flow cell repair device can achieve an automated process for determining the damage type of the sequencing flow cell.

[0159] As an optional implementation, based on the above embodiment, determining the damage type of the target sequencing flow cell based on the image includes:

[0160] Use a preset damage type determination model to determine the damage type of the target sequencing flow cell based on the image;

[0161] If the image includes surface scratches and / or structural deformation, determining that the damage type of the target sequencing flow cell is the second repairable type;

[0162] If the image does not include surface scratches and / or structural deformation, the damage type of the target sequencing flow cell is determined to be the first repairable type.

[0163] Among them, the preset damage type determination model refers to a model pre-configured in the repair device of the sequencing flow cell, which is used to determine the damage type of the target sequencing flow cell based on an image, such as an open source deep learning model.

[0164] Specifically, after receiving the image of the target sequencing flow cell, it is input into a preset damage type determination model. The preset damage type determination model can determine whether the current image includes surface scratches and / or structural deformations based on pre-trained memory. If the image includes surface scratches and / or structural deformations, the damage type of the target sequencing flow cell is determined to be the second repairable type. If the image does not include surface scratches and / or structural deformations, the damage type of the target sequencing flow cell is determined to be the first repairable type.

[0165] It can be understood that the preset damage type determination model is a trained model obtained through a large amount of image recognition learning.

[0166] The repair method for a sequencing flow cell provided in an embodiment of the present application determines the damage type of a target sequencing flow cell based on an image, including: using a preset damage type determination model to determine the damage type of the target sequencing flow cell based on an image; if the image includes surface scratches and / or structural deformations, then determining the damage type of the target sequencing flow cell to be the second repairable type; if the image does not include surface scratches and / or structural deformations, then determining the damage type of the target sequencing flow cell to be the first repairable type. The preset damage type determination model can quickly analyze and judge based on the input image, which greatly shortens the classification time compared to manual inspection that requires experience and long-term observation; the preset damage type determination model analyzes the image based on unified standards and algorithms to ensure the consistency and accuracy of the results; by determining whether the image includes surface scratches and / or structural deformations, the damage type is clearly divided into the second repairable type and the first repairable type, providing clear guidance for the repair work, thereby improving the success rate of the repair.

[0167] As an optional implementation, based on the above embodiment, the training steps of the damage type determination model are preset, including:

[0168] Obtaining a training sample set, the training sample set including image samples of a plurality of sequencing flow cells, the image samples of the plurality of sequencing flow cells including samples including surface scratches and / or structural deformations in the images and samples not including surface scratches and structural deformations in the images;

[0169] The initial damage type determination model is trained using a training sample set;

[0170] In response to the model convergence condition being satisfied, the initial damage type determination model satisfying the model convergence condition is determined as the preset damage type determination model.

[0171] Specifically, after using a computer vision sensor to collect image samples of multiple sequencing flow cells, the surface scratches and / or structural deformations therein are marked out and merged with the images that do not include surface scratches and structural deformations as a training sample set. The training sample set is input into the initial damage type determination model so that the initial damage type determination model can learn and determine the image features including surface scratches and / or structural deformations based on the training sample set. During the training process, parameters can be adjusted in a timely manner, and it can be determined whether the model convergence conditions are met. When the model convergence conditions are met, the initial damage type determination model that meets the model convergence conditions is determined as the preset damage type determination model.

[0172] The model convergence condition may be that the number of iterations reaches a preset number of iterations, or other model convergence conditions, which are not limited in this embodiment.

[0173] The repair method for a sequencing flow cell provided in an embodiment of the present application includes a step of training a preset damage type determination model, comprising: obtaining a training sample set, the training sample set including a plurality of sequencing flow cell image samples, the plurality of sequencing flow cell image samples including samples including surface scratches and / or structural deformations in the images and samples not including surface scratches and structural deformations in the images; using the training sample set to train an initial damage type determination model; and in response to satisfying a model convergence condition, determining the initial damage type determination model that satisfies the model convergence condition as a preset damage type determination model. By training the initial damage type determination model with a training sample set including a plurality of sequencing flow cell image samples, wherein the plurality of sequencing flow cell image samples include samples including surface scratches and / or structural deformations in the images and samples not including surface scratches and structural deformations in the images, the model can learn the features including surface scratches and / or structural deformations in the images, thereby quickly determining whether the image includes surface scratches and / or structural deformations when determining the damage type, and further determining the damage type.

[0174] As an optional implementation manner, based on the above embodiment, the first preset temperature is 35°C to 45°C;

[0175] and / or, the enzymatic cleaning solution includes proteinase K and sodium lauryl sulfate;

[0176] And / or, the oxidizing cleaning solution includes peroxide water and citric acid.

[0177] The enzymatic cleaning solution may be a cleaning solution containing 0.1 mg / mL proteinase K, 0.05% sodium lauryl sulfate, and pH=7.5, or other proportions, which are not limited in this embodiment.

[0178] Wherein, the oxidizing cleaning liquid may be , 2% citric acid and pH=4.0 cleaning solution, or other ratios, which are not limited in this embodiment.

[0179] The repair method of the sequencing flow cell provided in the embodiment of the present application has a first preset temperature of 35°C to 45°C; and / or, the enzymatic cleaning solution includes proteinase K and sodium dodecyl sulfate; and / or, the oxidative cleaning solution includes water peroxide and citric acid. Controlling the temperature at 35°C to 45°C can achieve low-temperature cleaning, avoiding high temperature damage to the material structure of the sequencing flow cell; proteinase K is specifically used to cut protein residues in contaminants, and sodium dodecyl sulfate is used to assist in destroying the DNA-protein complex structure, thereby stripping the contamination layer; the combination of water peroxide and citric acid can effectively decompose fluorophores and residual metal ions, further remove trace contamination, and prevent interference from sequencing background signals.

[0180] As an optional implementation, based on the above embodiment, after determining the damage type of the target sequencing flow cell, the method further includes:

[0181] The controlled cleaning component removes the sealing film and sealant residues on the surface of the target sequencing flow cell.

[0182] The cleaning component may be a robotic arm including a cutter head, which is not limited in this embodiment.

[0183] Specifically, after determining the damage type of the target sequencing flow cell, the robot arm is controlled to control the pressure to Inside the flow cell, the robotic arm will use a dedicated blade to gently scrape off the sealing film, residual glue or label residue on the surface of the target sequencing flow cell.

[0184] The sequencing flow cell repair method provided in the embodiments of the present application, before determining the damage type of the target sequencing flow cell, further includes controlling a cleaning component to remove residual sealing film and sealant from the surface of the target sequencing flow cell. Residual sealing film and sealant may partially cover key areas of the sequencing flow cell, making it difficult to accurately identify the damage state of the sequencing flow cell and potentially causing secondary damage during subsequent processing. Removing these residues can improve the accuracy of damage type determination and the success rate of repair.

[0185] As an optional implementation, based on the above embodiment, after controlling the corresponding operating component to repair the target sequencing flow cell based on the repair scheme to obtain a repaired sequencing flow cell, the method further includes:

[0186] Controlling the optical detector and sequencing verifier to detect the repaired sequencing flow cell;

[0187] receiving a second detection result sent by the optical detector and the sequencing verification instrument;

[0188] determining whether the repair is successful according to the second detection result;

[0189] The detection indicators include at least two of the following:

[0190] Flatness, residue concentration, transmittance, surface roughness, output data volume, and base error rate.

[0191] The second detection result refers to the detection data sent by the optical detector and the sequencing verifier to verify whether the repair is successful.

[0192] Specifically, after obtaining the repaired sequencing flow cell, the grabbing component is controlled to move the repaired sequencing flow cell to the optical detector, and the optical detector is controlled to detect the surface of the repaired sequencing flow cell. After the detection is completed, the grabbing component is controlled to move the repaired sequencing flow cell to the sequencing verification instrument, and the sequencing verification instrument is controlled to detect the repaired sequencing flow cell using a standard human genome DNA sample as a template. After the detection is completed, the optical detector and the sequencing verification instrument send the detection results to the repair device of the sequencing flow cell. After receiving the second detection result, the repair device of the sequencing flow cell determines whether the repair is successful based on the second detection result, such as determining whether the surface roughness of the repaired sequencing flow cell meets a preset threshold, etc.

[0193] Optionally, the repair device of the sequencing flow cell may pre-store the test results of the optical detector and the sequencing verifier on a brand new sequencing flow cell under the same test conditions for comparison with the second test results to determine whether the repair is successful, or the threshold values ​​of each detection indicator may be pre-configured in the repair device of the sequencing flow cell to determine whether the repair is successful. This embodiment does not limit this.

[0194] Exemplarily, the surface roughness Ra difference is set to ≤0.01 μm, the transmittance is not less than 90% of the new sequencing flow cell, the output data volume is not less than 92% of the new sequencing flow cell, etc.

[0195] As shown in Table 1, the detection data of the new sequencing flow cell and the repaired sequencing flow cell are compared.

[0196] Table 1

[0197]

[0198] As can be seen from Table 1, the transmittance, surface roughness, and output data volume of the repaired sequencing flow cell are close to those of a new sequencing flow cell, which can prove that the sequencing flow cell repaired by the method provided in this application has good quality and function and is capable of repeated reuse.

[0199] The repair method of the sequencing flow cell provided in the embodiment of the present application controls the corresponding operating component to repair the target sequencing flow cell based on the repair scheme to obtain the repaired sequencing flow cell. The method also includes: controlling the optical detector and the sequencing verifier to detect the repaired sequencing flow cell; receiving the second detection result sent by the optical detector and the sequencing verifier; determining whether the repair is successful based on the second detection result; the detection indicators include at least two of the following: flatness, residue concentration, transmittance, surface roughness, output data volume, and base error rate. By controlling the optical detector and the sequencing verifier to detect and determining whether the repair is successful based on the detection results, unqualified sequencing flow cells can be discovered in time, ensuring the usability of the repaired sequencing flow cell. The detection indicators include at least two of flatness, residue concentration, transmittance, surface roughness, output data volume, and base error rate. The repair status of the repaired flow cell can be determined from multiple dimensions, thereby improving the reliability of the result of determining whether the repair is successful.

[0200] Figure 3 A flowchart of a repair method for a sequencing flow cell provided in another embodiment of the present application is shown in FIG. Figure 3 As shown, the repair method of the sequencing flow cell provided in this embodiment is a repair scheme including a cleaning process and a repair process, and the repair process includes specific steps of coating repair and hot pressing shaping. The drug target prediction method provided in this embodiment includes the following steps:

[0201] Step 301: Control the cleaning component to remove the sealing film and sealant residues on the surface of the target sequencing flow cell.

[0202] Step 302: Control the computer vision sensor to capture images of the target sequencing flow cell.

[0203] Step 303: Receive an image sent by a computer vision sensor, where the image includes the target sequencing flow cell.

[0204] Step 304 : Determine the damage type of the target sequencing flow cell based on the image using a preset damage type determination model.

[0205] In step 305 , in response to the image including surface scratches and structural deformation, it is determined that the damage type of the target sequencing flow cell is a second repairable type.

[0206] Step 306 : In response to determining that the second repairable type is the preset repairable type, determining the corresponding repair solution to be a cleaning process and a repair process.

[0207] Step 307: Control the temperature control component to set the temperature to a first preset temperature.

[0208] Step 308 , in response to reaching the first preset temperature, controlling the grabbing component to place the target sequencing flow cell into a cleaning container of an enzymatic cleaning solution, and using the enzymatic cleaning solution to clean the target sequencing flow cell.

[0209] In step 309, in response to determining that the target sequencing flow cell has been cleaned with the enzymatic cleaning solution for a first preset time, the grabbing component is controlled to move the target sequencing flow cell into a cleaning container of the oxidizing cleaning solution, and the ultrasonic component is controlled to turn on so that the target sequencing flow cell is cleaned with the oxidizing cleaning solution under an ultrasonic environment.

[0210] Step 310: Control the online UV absorbance detector and the fluorescence detector to detect the surface of the target sequencing flow cell. Step 311: Receive the first detection result sent by the online UV absorbance detector and the fluorescence detector.

[0211] Step 312: Determine the residue concentration of the target sequencing flow cell based on the first detection result.

[0212] Step 313 : In response to the residue concentration being less than a preset residue concentration threshold, controlling the capture component to place the target sequencing flow cell in a plasma surface activation processor for activation treatment.

[0213] Step 314 , in response to the activation treatment duration reaching a second preset duration, controlling the spray assembly to spray a repair liquid on the surface of the target sequencing flow cell.

[0214] In step 315, the grabbing assembly is controlled to place the target sequencing flow cell in a preset mold, and the temperature is controlled to be a second preset temperature and the pressure is controlled to be a preset pressure value, so as to perform hot pressing shaping on the target sequencing flow cell to obtain a repaired sequencing flow cell.

[0215] Step 316: Control the optical detector and the sequencing verifier to detect the repaired sequencing flow cell.

[0216] Step 317: Receive the second detection result sent by the optical detector and the sequencing verification instrument.

[0217] Step 318: Determine whether the repair is successful based on the second detection result.

[0218] It is understandable that if the residue concentration determined in step 312 is greater than the preset residue concentration threshold, steps 308 and 309 are repeated until the residue concentration is less than the preset residue concentration threshold.

[0219] In this embodiment, the implementation method and technical effects of steps 301 to 318 are similar to the implementation method of the corresponding solutions in the above embodiments, and will not be repeated here.

[0220] Figure 4A schematic diagram of the structure of a repair device for a sequencing flow cell according to an embodiment of the present application is shown in FIG. Figure 4 As shown, the repair device for the sequencing flow cell provided in this embodiment is located in the repair equipment for the sequencing flow cell. The repair device 40 for the sequencing flow cell provided in this embodiment includes: a determination module 41 and a control module 42.

[0221] Among them, the determination module 41 is used to determine the damage type of the target sequencing flow cell; the determination module 41 is also used to determine the corresponding repair scheme based on the preset repairable type if the damage type is a preset repairable type, and the repair scheme includes cleaning treatment or cleaning treatment and repair treatment; wherein, the cleaning treatment includes: at a first preset temperature, using an enzymatic cleaning solution to clean the target sequencing flow cell for a first preset time, and then using an oxidizing cleaning solution to clean the target sequencing flow cell until the residue concentration is less than the preset residue concentration threshold; the repair treatment includes: in response to the residue concentration being less than the preset residue concentration threshold, coating repair and / or hot pressing shaping the target sequencing flow cell; the control module 42 is used to control the corresponding operating component to operate the target sequencing flow cell based on the repair scheme to obtain a repaired sequencing flow cell.

[0222] The repair device for the sequencing flow cell provided in this embodiment can perform Figure 2 The specific implementation principles and technical effects of the methods provided in the embodiments are similar and will not be repeated here.

[0223] Optionally, the preset repairable type includes a first repairable type and a second repairable type, wherein the target sequencing flow cell of the first repairable type has no physical damage, and the target sequencing flow cell of the second repairable type has physical damage. The determination module 41, when determining the corresponding repair scheme based on the preset repairable type, is specifically used to: if the preset repairable type is the first repairable type, determine the corresponding repair scheme as cleaning treatment; if the preset repairable type is the second repairable type, determine the corresponding repair scheme as cleaning treatment and repair treatment.

[0224] Optionally, the repair scheme includes a cleaning process, and the control module 42, when controlling the corresponding operating component to operate the target sequencing flow cell based on the repair scheme, is specifically used to: control the temperature control component to set the temperature to a first preset temperature; in response to reaching the first preset temperature, control the grabbing component to place the target sequencing flow cell in a cleaning container of an enzymatic cleaning solution, and use the enzymatic cleaning solution to clean the target sequencing flow cell; in response to determining that the enzymatic cleaning solution has been used to clean the target sequencing flow cell for a first preset time, control the grabbing component to move the target sequencing flow cell to a cleaning container of an oxidative cleaning solution, and control the ultrasonic component to turn on, so that the target sequencing flow cell is cleaned by the oxidative cleaning solution under an ultrasonic environment.

[0225] Optionally, the cleaning process also includes determining the residue concentration of the target sequencing flow cell. The control module 42, when controlling the corresponding operating component to operate the target sequencing flow cell based on the repair plan, is specifically used to: control the online ultraviolet absorbance detector and the fluorescence detector to detect the surface of the target sequencing flow cell; receive the first detection result sent by the online ultraviolet absorbance detector and the fluorescence detector; and determine the residue concentration of the target sequencing flow cell based on the first detection result.

[0226] Optionally, the repair scheme includes a repair process, and the control module 42, when controlling the corresponding operating component to operate the target sequencing flow cell based on the repair scheme, is specifically used to: control the grabbing component to place the target sequencing flow cell in a plasma surface activation processor for activation treatment; in response to the activation treatment duration reaching a second preset duration, control the spraying component to spray the repair liquid on the surface of the target sequencing flow cell; and / or control the grabbing component to place the target sequencing flow cell in a preset mold, and control the temperature to be the second preset temperature, and the pressure to be the preset pressure value, so as to achieve hot pressing shaping of the target sequencing flow cell.

[0227] Optionally, the control module 42 is further used to control the computer vision sensor to capture images of the target sequencing flow cell; the determination module 41, when determining the damage type of the target sequencing flow cell, is specifically used to: receive an image sent by the computer vision sensor, the image including the target sequencing flow cell; and determine the damage type of the target sequencing flow cell based on the image.

[0228] Optionally, when determining the damage type of the target sequencing flow cell based on an image, the determination module 41 is specifically used to: determine the damage type of the target sequencing flow cell based on the image using a preset damage type determination model; if the image includes surface scratches and / or structural deformations, determine that the damage type of the target sequencing flow cell is the second repairable type; if the image does not include surface scratches and / or structural deformations, determine that the damage type of the target sequencing flow cell is the first repairable type.

[0229] Optionally, the repair device for the sequencing flow cell provided in this embodiment further includes a training module.

[0230] Accordingly, the training module is used to obtain a training sample set, which includes image samples of multiple sequencing flow cells, and the image samples of the multiple sequencing flow cells include samples in which the images include surface scratches and / or structural deformations and samples in which the images do not include surface scratches and structural deformations; use the training sample set to train the initial damage type determination model; in response to meeting the model convergence condition, determine the initial damage type determination model that meets the model convergence condition as the preset damage type determination model.

[0231] Optionally, the first preset temperature is 34° C. to 44° C.; and / or the enzymatic cleaning solution includes proteinase K and sodium lauryl sulfate; and / or the oxidative cleaning solution includes peroxide water and citric acid.

[0232] Optionally, the control module 42 is further configured to control the cleaning component to remove the sealing film and sealant residues on the surface of the target sequencing flow cell.

[0233] Optionally, the repair device for the sequencing flow cell provided in this embodiment further includes a receiving module.

[0234] Accordingly, the control module 42 is further configured to control the optical detector and the sequencing verifier to detect the repaired sequencing flow cell; the receiving module is configured to receive a second detection result sent by the optical detector and the sequencing verifier; the determination module 41 is further configured to determine whether the repair is successful based on the second detection result; the detection indicators include at least one of the following: flatness, residue concentration, transmittance, surface roughness, output data volume, and base error rate.

[0235] Figure 5 A schematic diagram of the structure of a repair device for a sequencing flow cell according to an embodiment of the present application is shown in FIG. Figure 5 As shown, the repair device 50 for the sequencing flow cell provided in this embodiment includes: a processor 51 and a memory 52 communicatively connected to the processor.

[0236] Memory 52 is used to store computer-executable instructions; processor 51 executes the computer-executable instructions stored in memory 52 to implement the sequencing flow cell repair method provided in the above embodiment. For further explanation, please refer to the corresponding descriptions and effects of the steps in the accompanying drawings, and detailed description is omitted here.

[0237] The program may include program code, which includes computer-executable instructions. The memory 52 may include a high-speed RAM memory, or may also include a non-volatile memory, such as at least one disk memory.

[0238] In this embodiment, the processor 51 and the memory 52 are connected via a bus. The bus may be an Industry Standard Architecture (ISA) bus, a Peripheral Component Interconnect (PCI) bus, or an Extended Industry Standard Architecture (EISA) bus. The bus can be divided into an address bus, a data bus, a control bus, etc. For ease of representation, Figure 5Only one thick line is used in the diagram, but this does not mean that there is only one bus or one type of bus.

[0239] An embodiment of the present application further provides a computer-readable storage medium, in which computer-executable instructions are stored. When a controller executes the computer-executable instructions, each step of the method in the above embodiment is implemented.

[0240] An embodiment of the present application further provides a computer program product, including a computer program, which implements each step of the method in the above embodiment when executed by a controller.

[0241] The various embodiments described above in this application can be implemented in digital electronic circuit systems, integrated circuit systems, field programmable gate arrays (FPGAs), application-specific integrated circuits (ASICs), application-specific standard parts (ASSPs), systems on chips (SOCs), complex programmable logic devices (CPLDs), computer hardware, firmware, software, and / or combinations thereof. These various embodiments may include: being implemented in one or more computer programs, which can be executed and / or interpreted on a programmable system including at least one programmable processor, which can be a special-purpose or general-purpose programmable processor, which can receive data and instructions from a storage system, at least one input device, and at least one output device, and transmit data and instructions to the storage system, the at least one input device, and the at least one output device.

[0242] The computer-executable instructions for implementing the methods of the present application may be written in any combination of one or more programming languages. These computer-executable instructions may be provided to a processor or controller of a general-purpose computer, a special-purpose computer, or other programmable data processing device, such that when the computer-executable instructions are executed by the processor or controller, the functions / operations specified in the flowcharts and / or block diagrams are implemented. The computer-executable instructions may be executed entirely on the machine, partially on the machine, as a stand-alone software package, partially on the machine and partially on a remote machine, or entirely on a remote machine or electronic device.

[0243] In the context of this application, a computer-readable storage medium may be a tangible medium that may contain or store a program for use by or in conjunction with an instruction execution system, apparatus, or device. A computer-readable storage medium may be a machine-readable signal medium or a machine-readable storage medium. A computer-readable storage medium may include, but is not limited to, an electronic, magnetic, optical, electromagnetic, infrared, or semiconductor system, apparatus, or device, or any suitable combination of the foregoing. More specific examples of computer-readable storage media include an electrical connection based on one or more wires, a portable computer disk, a hard disk, a random access memory (RAM), a read-only memory (ROM), an erasable programmable read-only memory (EPROM), an optical fiber, a portable compact disc read-only memory (CD-ROM), an optical storage device, a magnetic storage device, or any suitable combination of the foregoing. Alternatively, the computer-readable storage medium may include: Resistive Random Access Memory (RRAM), Dynamic Random Access Memory (DRAM), Static Random-Access Memory (SRAM), Enhanced Dynamic Random Access Memory (EDRAM), High-Bandwidth Memory (HBM), Hybrid Memory Cube (HMC), etc.

[0244] The systems and techniques described herein can be implemented in a computing system that includes back-end components (e.g., as data electronics), or a computing system that includes middleware components (e.g., application electronics), or a computing system that includes front-end components (e.g., a user computer with a graphical user interface or web browser through which a user can interact with implementations of the systems and techniques described herein), or a computing system that includes any combination of such back-end components, middleware components, or front-end components. The components of the system can be interconnected by any form or medium of digital data communication (e.g., a communication network). Examples of communication networks include a local area network (LAN), a wide area network (WAN), and the Internet.

[0245] It should be noted that, for the aforementioned method embodiments, for the sake of simplicity of description, they are all expressed as a series of action combinations, but those skilled in the art should be aware that the present application is not limited by the order of the actions described, because according to the present application, certain steps can be performed in other orders or simultaneously. Secondly, those skilled in the art should also be aware that the embodiments described in the specification are all optional embodiments, and the actions and modules involved are not necessarily required by the present application. In other words, the various forms of processes shown above can be used to reorder, add or delete steps. For example, the steps disclosed in the present application can be executed in parallel, can be executed sequentially, or can be executed in different orders. As long as the desired results of the technical solutions disclosed in the present application can be achieved, this document does not limit them here.

[0246] It should be further noted that, although the various steps in the flowchart are shown in sequence as indicated by the arrows, these steps are not necessarily performed in the order indicated by the arrows. Unless otherwise specified herein, there is no strict order restriction on the execution of these steps, and these steps may be performed in other orders. Moreover, at least a portion of the steps in the flowchart may include multiple sub-steps or multiple stages, and these sub-steps or stages are not necessarily performed at the same time, but may be performed at different times. The execution order of these sub-steps or stages is not necessarily to be performed in sequence, but may be performed in turn or alternately with other steps or at least a portion of the sub-steps or stages of other steps.

[0247] It should be understood that the above-described device embodiments are merely illustrative, and the device of the present application may also be implemented in other ways. For example, the division of units / modules in the above-described embodiments is merely a logical functional division, and actual implementations may employ other division methods. For example, multiple units, modules, or components may be combined or integrated into another system, or some features may be omitted or not implemented.

[0248] In addition, unless otherwise specified, the functional units / modules in the various embodiments of the present application may be integrated into a single unit / module, each unit / module may exist physically separately, or two or more units / modules may be integrated together. The aforementioned integrated units / modules may be implemented in the form of hardware or software program modules.

[0249] If the integrated unit / module is implemented in the form of hardware, the hardware may be a digital circuit, an analog circuit, etc. The physical implementation of the hardware structure includes but is not limited to transistors, memristors, etc.

[0250] If the integrated unit / module is implemented as a software program module and sold or used as an independent product, it can be stored in a computer-readable memory. Based on this understanding, the technical solution of this application, or the portion that contributes to the relevant technology, or all or part of the technical solution, can be embodied in the form of a software product. This computer software product is stored in a memory and includes a number of instructions for enabling a computer device (which can be a personal computer, server, or network device, etc.) to execute all or part of the steps of the various embodiments of the method of this application. The aforementioned memory includes various media that can store computer-executable instructions, such as USB flash drives, read-only memories (ROMs), random access memories (RAMs), mobile hard drives, magnetic disks, or optical disks.

[0251] In the above embodiments, the description of each embodiment has its own emphasis. For parts not described in detail in a particular embodiment, please refer to the relevant description of other embodiments. The technical features of the above embodiments can be combined in any way. To keep the description concise, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0252] Those skilled in the art will readily appreciate other embodiments of the present invention after considering the specification and practicing the invention disclosed herein. This application is intended to cover any variations, uses, or adaptations of the present invention that follow the general principles of this application and include common knowledge or customary techniques in the art not disclosed herein. The description and examples are to be considered as exemplary only.

[0253] It should be understood that the present application is not limited to the precise structure described above and shown in the accompanying drawings, and various modifications and changes can be made without departing from the scope thereof. Therefore, the above specific embodiments do not constitute a limitation on the scope of protection of the present application. It should be understood by those skilled in the art that various modifications, combinations, sub-combinations and substitutions can be made according to design requirements and other factors. Any modifications, equivalent substitutions and improvements made within the principles of the present application should be included in the scope of protection of the present application.

Claims

1. A method for repairing a sequencing flow cell, characterized in that: The method comprises: Acquire images of various surfaces of the sequencing flow cell of interest; Calculating pixel difference vectors between the reference sequencing flow cell image and the image of each surface of the target sequencing flow cell; Determine the damage type of the target sequencing flow cell based on the pixel difference calculation result and the preset difference threshold; if the damage type is a preset repairable type, determine the corresponding repair scheme based on the preset repairable type, and the repair scheme includes a cleaning process or a cleaning process and a repair process; wherein, the cleaning process includes: at a temperature of 35°C to 45°C, use proteinase K and sodium dodecyl sulfate cleaning solution to clean the target sequencing flow cell for a first preset time, and then use peroxide water and citric acid cleaning solution to clean the target sequencing flow cell, and determine the residue concentration of the target sequencing flow cell until the residue concentration is less than the preset residue concentration threshold; the repair process includes: in response to the residue concentration being less than the preset residue concentration threshold, after activating the target sequencing flow cell, use a silicon-based nano-repair solution to perform coating repair and / or hot pressing shaping; Controlling a corresponding operating component to operate the target sequencing flow cell based on the repair scheme to obtain a repaired sequencing flow cell; Controlling an optical detector and a sequencing verifier to detect the repaired sequencing flow cell; receiving a second detection result sent by the optical detector and the sequencing verification instrument; Determining whether the repair is successful based on the values ​​of the detection indicators in the second detection result and the preset thresholds corresponding to the detection indicators; When determining the residue concentration of the target sequencing flow cell, controlling the corresponding operating component to operate the target sequencing flow cell based on the repair scheme includes: Controlling an online ultraviolet absorbance detector and a fluorescence detector to detect the surface of the target sequencing flow cell; receiving first detection results sent by the online ultraviolet absorption detector and the fluorescence detector; Determine the residue concentration of the target sequencing flow cell based on the first detection result.

2. The method according to claim 1, characterized in that The preset repairable type includes a first repairable type and a second repairable type, wherein the target sequencing flow cell of the first repairable type has no physical damage, and the target sequencing flow cell of the second repairable type has physical damage; and determining a corresponding repair solution based on the preset repairable type includes: If the preset repairable type is the first repairable type, determining that the corresponding repair solution is a cleaning process; If the preset repairable type is the second repairable type, the corresponding repair solution is determined to be a cleaning process and a repair process.

3. The method according to claim 1, characterized in that The repair scheme includes a cleaning process, and controlling a corresponding operating component to operate the target sequencing flow cell based on the repair scheme includes: Controlling the temperature control component to set the temperature to a first preset temperature; In response to reaching the first preset temperature, controlling the grabbing component to place the target sequencing flow cell in a cleaning container of the proteinase K and sodium dodecyl sulfate cleaning solution, and cleaning the target sequencing flow cell with the proteinase K and sodium dodecyl sulfate cleaning solution; In response to determining that the target sequencing flow cell has been cleaned using proteinase K and sodium dodecyl sulfate cleaning solution for a first preset time, the grabbing component is controlled to move the target sequencing flow cell into the cleaning container of the peroxide water and citric acid cleaning solution, and the ultrasonic component is controlled to turn on so that the target sequencing flow cell is cleaned by the peroxide water and citric acid cleaning solution under an ultrasonic environment.

4. The method according to claim 1, wherein The repair scheme includes a repair process, and controlling a corresponding operating component to operate the target sequencing flow cell based on the repair scheme includes: Controlling the grabbing component to place the target sequencing flow cell in a plasma surface activation processor for activation treatment; In response to the activation treatment duration reaching a second preset duration, controlling the spraying component to spray the repair liquid on the surface of the target sequencing flow cell; And / or, control the grabbing component to place the target sequencing flow cell in a preset mold, and control the temperature to be a second preset temperature and the pressure to be a preset pressure value to achieve hot pressing shaping of the target sequencing flow cell.

5. The method according to claim 1, wherein Before determining the damage type of the target sequencing flow cell, the method further includes: The cleaning component is controlled to remove the sealing film and sealant residues on the surface of the target sequencing flow cell.

6. The method according to claim 1, characterized in that The detection indicators include at least two of the following: Flatness, residue concentration, transmittance, surface roughness, output data volume, and base error rate.

7. A repair device for a sequencing flow cell, characterized in that: The device comprises: an acquisition module, configured to acquire images of various surfaces of a target sequencing flow cell; a calculation module for calculating pixel difference vectors between the reference sequencing flow cell image and the images of each surface of the target sequencing flow cell; a determination module, configured to determine a damage type of the target sequencing flow cell based on a pixel difference calculation result and a preset difference threshold; The determination module is further configured to, if the damage type is a preset repairable type, determine a corresponding repair scheme based on the preset repairable type, the repair scheme including a cleaning process or a cleaning process and a repair process; wherein the cleaning process includes: cleaning the target sequencing flow cell with proteinase K and sodium dodecyl sulfate cleaning solution at a temperature of 35°C to 45°C for a first preset time period, then cleaning the target sequencing flow cell with peroxide water and citric acid cleaning solution, and determining the residue concentration of the target sequencing flow cell until the residue concentration is less than a preset residue concentration threshold; the repair process includes: in response to the residue concentration being less than the preset residue concentration threshold, performing an activation process on the target sequencing flow cell, and then performing coating repair and / or hot pressing shaping with a silicon-based nano-repair solution; A control module, configured to control a corresponding operating component to operate the target sequencing flow cell based on the repair scheme to obtain a repaired sequencing flow cell; The control module is further used to control the optical detector and the sequencing verifier to detect the repaired sequencing flow cell; A receiving module, configured to receive the second detection result sent by the optical detector and the sequencing verifier; The determining module is further configured to determine whether the repair is successful based on the values ​​of the detection indicators in the second detection result and the preset thresholds corresponding to the detection indicators; The control module is further used to control the online ultraviolet absorbance detector and the fluorescence detector to detect the surface of the target sequencing flow cell when determining the residue concentration of the target sequencing flow cell; receive the first detection results sent by the online ultraviolet absorbance detector and the fluorescence detector; and determine the residue concentration of the target sequencing flow cell based on the first detection results.

8. A repair device for a sequencing flow cell, characterized in that: The device includes: a processor, and a memory communicatively connected to the processor; The memory is used to store computer-executable instructions; The processor is configured to execute the computer-executable instructions stored in the memory to implement the method according to any one of claims 1 to 6.

9. A computer-readable storage medium, characterized in that The computer-readable storage medium stores computer-executable instructions, which are used to implement the method according to any one of claims 1 to 6 when executed by a processor.

10. A computer program product, comprising a computer program, wherein when the computer program is executed by a processor, the method according to any one of claims 1 to 6 is implemented.

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