Method for detecting hematocrit

Abstract

The invention relates to a hematocrit detection method, which comprises the following steps: after determining that a to-be-detected blood sample is completely injected, continuously and alternately applying a plurality of disturbance signals with different frequencies to an HCT electrode within a first preset duration, after a second preset duration, applying a detection signal to the HCT electrode, obtaining the impedance of the to-be-detected blood sample, and determining the hematocrit according to the impedance and a preset equation. According to the method and the device, the disturbance signals with different frequencies are continuously and alternately applied to the HCT electrode within the first preset duration, so that bubbles in the sample can be uniformly distributed, the precision of the detected impedance can be effectively improved under the condition that the bubbles exist in the sample, and the accuracy of the detected impedance is improved. Therefore, the detection accuracy of the hematocrit can be effectively improved.

Description

Technical Field

[0001] This application belongs to the technical field of electrochemical biosensing, and particularly relates to a method for detecting hematocrit. Background Art

[0002] Hematocrit is an important parameter for characterizing blood samples in medical tests and an important auxiliary determination data in the measurement and utilization of blood viscosity.

[0003] Currently, the conventional method for detecting hematocrit generally uses impedance detection. After the injection of the blood sample to be tested is completed, a detection signal is applied to the HCT electrode of the test strip to obtain the impedance of the blood sample to be tested. According to the detected impedance and a preset equation, the hematocrit of the blood sample to be tested is obtained. Usually, during the injection process of the blood sample to be tested, the sample will be mixed with some air and enter the injection channel, and these air bubbles will randomly appear at any position in the injection channel, resulting in the presence of bubbles in the sample and uneven distribution of the bubbles. Due to the uneven distribution of the bubbles in the sample, the precision of the detected impedance is poor, thereby resulting in low accuracy of the detected hematocrit.

[0004] Therefore, in the case of the presence of bubbles in the sample, how to improve the detection accuracy of hematocrit is a technical problem that needs to be urgently solved by those skilled in the art. Summary of the Invention

[0005] The purpose of this application is to provide a method for detecting hematocrit, which can effectively improve the precision of the detected impedance in the case of bubbles in the sample, thereby effectively improving the detection accuracy of hematocrit.

[0006] The technical solution provided by this application is as follows: A method for detecting hematocrit, comprising: After determining that the injection of the blood sample to be tested is completed, continuously apply a plurality of disturbance signals with different frequencies to the HCT electrode alternately within a first preset time period; After an interval of a second preset time period, apply a detection signal to the HCT electrode; Obtain the impedance of the blood sample to be tested; According to the impedance and a preset equation, obtain the hematocrit of the blood sample to be tested.

[0007] Optionally, the disturbance signal is a sine wave AC signal, and the frequency range is 4 Hz to 1 kHz.

[0008] Optionally, the plurality of different frequencies of the disturbance signal at least include two frequencies located at both ends of the frequency range.

[0009] Optionally, the application duration of the signal with the highest frequency among the multiple perturbation signals with different frequencies is greater than 50% of the first preset duration.

[0010] Optionally, after determining that the injection of the blood sample to be measured is completed, multiple perturbation signals with different frequencies are continuously applied to the HCT electrode within the first preset duration, including: After determining that the injection of the blood sample to be measured is completed, a perturbation signal with a first preset frequency and a perturbation signal with a second preset frequency are continuously applied to the HCT electrode within the first preset duration, where the first preset frequency is less than the second preset frequency.

[0011] Optionally, after determining that the injection of the blood sample to be measured is completed, a perturbation signal with a first preset frequency and a perturbation signal with a second preset frequency are continuously applied to the HCT electrode within the first preset duration, including: After determining that the injection of the blood sample to be measured is completed, perform an alternating operation for a first preset number of times. Each alternating operation includes: applying a perturbation signal with a first preset frequency to the HCT electrode for a first preset sub-duration, and then applying a perturbation signal with a second preset frequency to the HCT electrode for a second preset sub-duration; where the second preset sub-duration is greater than the first preset sub-duration.

[0012] Optionally, after performing the alternating operation for the first preset number of times, an additional operation is further included. The additional operation includes: applying a perturbation signal with a first preset frequency to the HCT electrode for a third preset sub-duration, and then applying a perturbation signal with a second preset frequency to the HCT electrode for a fourth preset sub-duration; where the fourth preset sub-duration is greater than the third preset sub-duration.

[0013] Optionally, after determining that the injection of the blood sample to be measured is completed, multiple perturbation signals with different frequencies are continuously applied to the HCT electrode within the first preset duration, including: After determining that the injection of the blood sample to be measured is completed, a perturbation signal with a third preset frequency, a perturbation signal with a fourth preset frequency, and a perturbation signal with a fifth preset frequency are continuously applied to the HCT electrode within the first preset duration, where the third preset frequency is less than the fourth preset frequency, and the fourth preset frequency is less than the fifth preset frequency.

[0014] Optionally, after determining that the injection of the blood sample to be measured is completed, a perturbation signal with a third preset frequency, a perturbation signal with a fourth preset frequency, and a perturbation signal with a fifth preset frequency are continuously applied to the HCT electrode within the first preset duration, including: After determining that the injection of the blood sample to be tested is completed, perform the second preset number of alternating operations. Each alternating operation includes: first applying a perturbation signal with a third preset frequency to the HCT electrode for a fifth preset sub-duration, then applying a perturbation signal with a fourth preset frequency to the HCT electrode for a sixth preset sub-duration, and then applying a perturbation signal with a fifth preset frequency to the HCT electrode for a seventh preset sub-duration.

[0015] Optionally, the value of the fourth preset frequency is close to the median between the third preset frequency and the fifth preset frequency.

[0016] Compared with the prior art, a method for detecting hematocrit provided by the present application, after determining that the injection of the blood sample to be tested is completed, continuously apply multiple perturbation signals with different frequencies to the HCT electrode alternately within a first preset duration. After an interval of a second preset duration, apply a detection signal to the HCT electrode to obtain the impedance of the blood sample to be tested. According to the impedance and a preset equation, the hematocrit of the blood sample to be tested is obtained. In the present application, by continuously applying multiple perturbation signals with different frequencies to the HCT electrode alternately within the first preset duration, the bubbles in the sample can be evenly distributed, and thus the precision of the detected impedance can be effectively improved in the case of bubbles in the sample, thereby effectively improving the detection accuracy of hematocrit. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the following will briefly introduce the drawings required for the description of the embodiments or the prior art. Obviously, the drawings in the following description are only some embodiments of the present application. For those of ordinary skill in the art, without creative efforts, other drawings can be obtained based on these drawings.

[0018] Figure 1 It is a schematic flowchart of a method for detecting hematocrit disclosed in an embodiment of the present application. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0019] In order to enable those skilled in the art to better understand the technical solutions in the present application, the following will clearly and completely describe the technical solutions in the embodiments of the present application. Obviously, the described embodiments are only some embodiments of the present application, rather than all embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the scope of protection of the present application.

[0020] It should be noted that when an element is referred to as "fixed to" or "disposed on" another element, it can be directly on the other element or indirectly disposed on the other element; when an element is referred to as "connected to" another element, it can be directly connected to the other element or indirectly connected to the other element.

[0021] It should be understood that the orientation or positional relationship indicated by the terms "length", "width", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. is based on the orientation or positional relationship shown in the drawings. It is only for the convenience of describing the present application and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation to the present application.

[0022] In addition, the terms "first" and "second" are only used for descriptive purposes and cannot be construed as indicating or implying relative importance or implicitly specifying the quantity of the indicated technical features. Thus, the features defined with "first" and "second" may explicitly or implicitly include one or more of such features. In the description of the present application, the meaning of "a plurality of" and "several" is two or more, unless otherwise specifically defined.

[0023] It should be noted that the structures, proportions, sizes, etc. shown in the drawings of this specification are only used to cooperate with the content disclosed in the specification for those skilled in the art to understand and read, and are not used to limit the implementation conditions of the present application. Therefore, they do not have technical substantive significance. Any modification of the structure, change of the proportional relationship or adjustment of the size, without affecting the effects that the present application can produce and the purposes that can be achieved, should still fall within the scope covered by the technical content disclosed in the present application.

[0024] As Figure 1 shown, an embodiment of the present application provides a method for detecting hematocrit, including: S1. After determining that the injection of the blood sample to be tested is completed, continuously apply a plurality of disturbance signals with different frequencies to the HCT electrode alternately within a first preset time period; In this embodiment, a blood glucose meter and a supporting test strip are used as the test equipment. The first preset time period is a preset time period. After determining that a blood sample enters the injection channel of the test strip, injection judgment can be first performed to determine whether the test strip has been filled with the blood sample to be tested. If it is determined that the test strip has been filled with the blood sample to be tested, that is, after determining that the injection of the blood sample to be tested is completed, continuously apply a plurality of disturbance signals with different frequencies to the HCT electrode alternately within the first preset time period to disturb the unevenly distributed bubbles in the sample.

[0025] S2. After an interval of a second preset duration, apply a detection signal to the HCT electrode; In this embodiment, the second preset duration is a preset duration. It can be that after an interval of the second preset duration, a detection signal with a preset amplitude and a sixth preset frequency is continuously applied to the HCT electrode within a third preset duration. Here, the third preset duration is a preset duration, the preset amplitude is a preset amplitude, and the sixth preset frequency is a preset frequency.

[0026] Each preset duration can be determined according to the detection timing of the detection index, and the preset durations for different detection indexes may be different. In this embodiment, specifically, the second preset duration can be 5 s, the third preset duration can be 300 ms or 280 ms, the preset amplitude can be 100 mV, and the sixth preset frequency can be 10 KHz or 200 KHz. The detection signal can be a sinusoidal alternating current signal. More specifically, it can be that after an interval of 5 s, a sinusoidal alternating current signal with an amplitude of 100 mV and a frequency of 10 KHz is continuously applied to the HCT electrode within 300 ms, or it can be that after an interval of 5 s, a sinusoidal alternating current signal with an amplitude of 100 mV and a frequency of 200 KHz is continuously applied to the HCT electrode within 280 ms.

[0027] S3. Obtain the impedance of the blood sample to be measured; In this embodiment, the impedance of the blood sample to be measured can be obtained when a detection signal with a preset amplitude and a sixth preset frequency is continuously applied to the HCT electrode within the third preset duration.

[0028] S4. According to the impedance and a preset equation, obtain the hematocrit of the blood sample to be measured.

[0029] In this embodiment, the preset equation is a preset equation. Specifically, the preset equation is a linear equation of impedance and hematocrit. Substitute the detected impedance into the preset equation for calculation to obtain the calculated hematocrit of the blood sample to be measured. Substituting the detected sample impedance into the preset equation to calculate the HCT of the sample belongs to the conventional technology in this industry and has also been publicly disclosed in many documents, belonging to the well-known prior art.

[0030] In this embodiment, after alternately applying a plurality of perturbation signals with different frequencies to the HCT electrode, within the interval of the second preset duration, the bubbles will gradually be evenly distributed. That is, the plurality of perturbation signals with different frequencies can provide driving forces for the bubbles to cause some displacements of the bubbles. At the end of the second preset duration, the movement of the bubbles gradually stops and reaches a state of balanced position distribution. Then, after an interval of the second preset duration, a detection signal is applied to the HCT electrode again to detect the impedance of the blood sample to be measured, so that the accuracy of the detection result of the hematocrit obtained according to the impedance and the preset equation is higher.

[0031] Compared with the prior art, a method for detecting hematocrit provided by the present application determines that after the injection of the blood sample to be tested is completed, a plurality of disturbance signals with different frequencies are continuously and alternately applied to the HCT electrode within a first preset time period. After an interval of a second preset time period, a detection signal is applied to the HCT electrode to obtain the impedance of the blood sample to be tested. According to the impedance and a preset equation, the hematocrit of the blood sample to be tested is obtained. In the present application, by continuously and alternately applying a plurality of disturbance signals with different frequencies to the HCT electrode within the first preset time period, the bubble distribution in the sample can be made uniform, and thus the precision of the detected impedance can be effectively improved in the case of bubbles in the sample, so that the detection accuracy of hematocrit can be effectively improved.

[0032] As an implementation manner, in the embodiment of the present application, the disturbance signal is a sine wave alternating current signal, and the frequency range is 4 Hz to 1 kHz.

[0033] In this embodiment, because the sine wave has a smooth periodic change characteristic, it can generate a uniform and continuous driving force on the bubbles, and can avoid the instantaneous impact force that may be caused by a square wave or a pulse signal, so as to more controllably cause the bubbles to have a small displacement.

[0034] In this embodiment, by setting the frequency range of the disturbance signal to be 4 Hz to 1 kHz, it can not only overcome the static resistance of the bubbles to provide a driving force to cause the bubbles to displace, but also effectively avoid the violent oscillation and rupture of the bubbles caused by too high a frequency, and can reduce the risk of interference caused by the rupture of the bubbles to the detection of hematocrit. After the bubbles rupture, if the gas overflows from the sample, the volume of the sample will change, resulting in a change in the injection volume of the sample, which may cause errors in all subsequent detections; if the gas does not overflow from the sample, new bubbles will be formed at other positions, resulting in uneven bubble distribution. Therefore, try to select a suitable frequency to avoid the uncontrollable risk caused by the rupture of the bubbles.

[0035] As an implementation manner, in the embodiment of the present application, the plurality of different frequencies of the disturbance signal at least include two frequencies located at both ends of the frequency range respectively.

[0036] In this embodiment, at least two frequencies 4 Hz and 1 kHz located at both ends of the frequency range 4 Hz to 1 kHz are included in the multiple different frequencies of the disturbance signal. Specifically, the multiple different frequencies of the disturbance signal may include two frequencies 4 Hz and 1 kHz located at both ends of the frequency range 4 Hz to 1 kHz, or the multiple different frequencies of the disturbance signal may include two frequencies 4 Hz and 1 kHz located at both ends of the frequency range 4 Hz to 1 kHz, as well as one or more frequencies between 4 Hz and 1 kHz. Since the disturbance signals of different frequencies can drive the movement of bubbles of different sizes, by alternately disturbing with the disturbance signals of different frequencies, the dynamic response characteristics of bubbles of different sizes in the blood sample can be covered, and bubbles of different sizes can be driven to make different displacements, thereby further enabling the bubbles in the sample to be evenly distributed.

[0037] As an implementation manner, in the embodiment of the present application, the application duration of the signal with the highest frequency among the multiple different frequencies of the disturbance signals is greater than 50% of the first preset duration.

[0038] In this embodiment, generally, the signal with the highest frequency (such as 1 kHz) can drive microbubbles. Since microbubbles are more difficult to disperse and require longer vibration time, by making the application duration of the signal with the highest frequency greater than 50% of the first preset duration, the microbubbles can be effectively moved and dispersed, thereby further enabling the bubbles in the sample to be evenly distributed.

[0039] As an implementation manner, in the embodiment of the present application, step S1 includes: S11. After determining that the sampling of the blood sample to be tested is completed, alternately apply a disturbance signal with a first preset frequency and a disturbance signal with a second preset frequency to the HCT electrode within the first preset duration, where the first preset frequency is less than the second preset frequency.

[0040] In this embodiment, the first preset frequency and the second preset frequency are preset frequencies. Since the disturbance signal with the lower first preset frequency can overcome the static adhesion force of larger bubbles and the liquid viscous resistance, making them move slowly, and the disturbance signal with the higher second preset frequency can stimulate the resonance effect of microbubbles, break their adhesion to blood cells or the tube wall, and drive their movement. By first applying the disturbance signal with the lower first preset frequency and then applying the disturbance signal with the higher second preset frequency, larger bubbles can be driven to move first, and then microbubbles can be driven to move, which can avoid the risk of more microbubbles being generated due to the rupture of larger bubbles caused by local pressure changes when directly applying the disturbance signal with the higher second preset frequency, and can further enable the bubbles in the sample to be evenly distributed.

[0041] In this embodiment, specifically, the first preset frequency is 4 Hz and the second preset frequency is 1 kHz.

[0042] As an implementation manner, in the embodiments of the present application, step S11 includes: S111. After determining that the injection of the blood sample to be tested is completed, perform an alternating operation for a first preset number of times. Each alternating operation includes: applying a disturbance signal with a first preset frequency to the HCT electrode for a first preset sub-duration, and then applying a disturbance signal with a second preset frequency to the HCT electrode for a second preset sub-duration; Wherein, the second preset sub-duration is greater than the first preset sub-duration.

[0043] In this embodiment, the first preset number of times is a preset number of times, and the first preset sub-duration and the second preset sub-duration are preset durations.

[0044] In this embodiment, after determining that the injection of the blood sample to be tested is completed, by performing an alternating operation for a first preset number of times, each alternating operation includes applying a disturbance signal with a first preset frequency to the HCT electrode for a first preset sub-duration, and then applying a disturbance signal with a second preset frequency to the HCT electrode for a second preset sub-duration, it is possible to initially drive the large bubbles and micro-bubbles in the blood sample, so that most of the bubbles in the blood sample move, and the bubble distribution in the blood sample can be made uniform.

[0045] As an implementation manner, in the embodiments of the present application, after performing the alternating operation for the first preset number of times, S112 is further included: performing an additional operation once. The additional operation includes: applying a disturbance signal with a first preset frequency to the HCT electrode for a third preset sub-duration, and then applying a disturbance signal with a second preset frequency to the HCT electrode for a fourth preset sub-duration; wherein, the fourth preset sub-duration is greater than the third preset sub-duration.

[0046] In this embodiment, the third preset sub-duration and the fourth preset sub-duration are preset durations, and the first preset duration = (the first preset sub-duration + the second preset sub-duration) * the first preset number of times + the third preset sub-duration + the fourth preset sub-duration. By performing the additional operation including applying a disturbance signal with a first preset frequency to the HCT electrode for a third preset sub-duration, and then applying a disturbance signal with a second preset frequency to the HCT electrode for a fourth preset sub-duration, it is possible to perform an enhanced treatment on the more stubborn bubbles or local aggregation regions in the blood sample, and further improve the uniformity of the bubble distribution in the blood sample.

[0047] In this embodiment, specifically, the first preset number of times is 2 times, the first preset duration is 1 s, the first preset sub-duration is 100 ms, the second preset sub-duration is 200 ms, the third preset sub-duration is 100 ms, and the fourth preset sub-duration is 300 ms.

[0048] As an implementation manner, in the embodiments of the present application, step S1 includes: S21. After determining that the injection of the blood sample to be measured is completed, a disturbance signal with a third preset frequency, a disturbance signal with a fourth preset frequency, and a disturbance signal with a fifth preset frequency are alternately applied to the HCT electrode continuously within a first preset duration, where the third preset frequency is less than the fourth preset frequency, and the fourth preset frequency is less than the fifth preset frequency.

[0049] In this embodiment, the third preset frequency, the fourth preset frequency, and the fifth preset frequency are preset frequencies. Since the disturbance signal with the relatively low third preset frequency can overcome the static adhesion force of bubbles with relatively large sizes and the liquid viscous resistance, causing them to move slowly, the disturbance signal with the medium fourth preset frequency can excite the resonance oscillation of medium-sized bubbles, break the adsorption force between the bubbles, and drive them to move, and the disturbance signal with the relatively high fifth preset frequency can excite the resonance effect of microbubbles, break their adhesion to blood cells or the tube wall, and drive them to move. By first applying the disturbance signal with the relatively low third preset frequency, then applying the disturbance signal with the medium fourth preset frequency, and finally applying the disturbance signal with the relatively high fifth preset frequency, it is possible to first drive the relatively large-sized bubbles to move, then drive the medium-sized bubbles to move, and finally drive the microbubbles to move, which can avoid the risk of more microbubbles being generated due to the rupture of relatively large-sized bubbles caused by local pressure changes when directly applying the disturbance signal with the relatively high fifth preset frequency, and can further evenly distribute the bubbles in the sample.

[0050] As an implementation manner, in the embodiment of the present application, step S21 includes: S211. After determining that the injection of the blood sample to be measured is completed, perform an alternating operation for a second preset number of times. Each alternating operation includes: first applying a disturbance signal with a third preset frequency to the HCT electrode for a fifth preset sub-duration, then applying a disturbance signal with a fourth preset frequency to the HCT electrode for a sixth preset sub-duration, and then applying a disturbance signal with a fifth preset frequency to the HCT electrode for a seventh preset sub-duration.

[0051] In this embodiment, the second preset number of times is a preset number of times, the fifth preset sub-duration, the sixth preset sub-duration, and the seventh preset sub-duration are preset durations, and the first preset duration = (the fifth preset sub-duration + the sixth preset sub-duration + the seventh preset sub-duration) * the second preset number of times. After determining that the injection of the blood sample to be measured is completed, by performing the alternating operation for the second preset number of times, each alternating operation includes first applying a disturbance signal with a third preset frequency to the HCT electrode for the fifth preset sub-duration, then applying a disturbance signal with a fourth preset frequency to the HCT electrode for the sixth preset sub-duration, and then applying a disturbance signal with a fifth preset frequency to the HCT electrode for the seventh preset sub-duration, which can effectively drive large bubbles, medium bubbles, and small bubbles in the blood sample, causing bubbles of different sizes in the blood sample to move, and can effectively improve the uniformity of the bubble distribution in the blood sample.

[0052] In this embodiment, specifically, the second preset number of times is 2 times, the fifth preset sub-duration is 100 ms, the sixth preset sub-duration is 100 ms, and the seventh preset sub-duration is 300 ms.

[0053] As an implementation manner, in the embodiment of the present application, the fourth preset frequency is close to the middle number between the third preset frequency and the fifth preset frequency.

[0054] In this embodiment, by setting the value of the fourth preset frequency to be close to the middle number between the third preset frequency and the fifth preset frequency, it is possible to drive the movement of bubbles with a size close to the middle value between the size of the large bubbles driven by the disturbance signal with the third preset frequency and the size of the small bubbles driven by the disturbance signal with the fifth preset frequency through the disturbance signal with the fourth preset frequency, and the uniformity of the bubble distribution in the blood sample can be further improved.

[0055] In this embodiment, specifically, the third preset frequency is 4 Hz, the fourth preset frequency is 500 Hz, and the fifth preset frequency is 1 kHz.

[0056] In the embodiment of the present application, an experiment is designed to verify the coefficient of variation of the impedance obtained by performing multiple tests without adding a disturbance signal and adding a disturbance signal, so as to verify the precision of the measured impedance. Specifically, this experiment is carried out under a total of 10 test blood sample conditions of 5 concentrations (S1, S2, S3, S4, S5) and 3 different hematocrits (HCT20%, HCT40%, HCT70%). The concentration range is 1.4 to 30.6 mmol / L, and there are 6 verification test conditions including 3 disturbance situations (no disturbance added, disturbance method one, disturbance method two) and 2 frequencies (10 kHz, 200 kHz) of the detection signal. Two tests are carried out under each verification test condition, and the data obtained are shown in Table 1 below: Table 1 Data obtained for various test blood samples under various validation test conditions

[0057] Among them, the frequency in the above table is the frequency of the detection signal; Disturbance mode 1 is to perform 2 alternating operations. Each alternating operation includes: applying a 4 Hz disturbance signal to the HCT electrode for 100 ms, then applying a 1 KHz disturbance signal to the HCT electrode for 200 ms, and performing an additional operation. The additional operation includes: applying a 4 Hz disturbance signal to the HCT electrode for 100 ms, then applying a 1 KHz disturbance signal to the HCT electrode for 300 ms; Disturbance mode 2 is to perform 2 alternating operations. Each alternating operation includes: first applying a 4 Hz disturbance signal to the HCT electrode for 100 ms, then applying a 500 Hz disturbance signal to the HCT electrode for 100 ms, and then applying a 1 KHz disturbance signal to the HCT electrode for 300 ms; Z is the test impedance of the test blood sample; CV is the coefficient of variation of a group (10) of impedances. As can be seen from Table 1, for test samples with the same HCT, under test conditions with the same frequency, the coefficient of variation CV obtained based on the test impedance Z has a lower value of the coefficient of variation CV of the test impedance with the addition of the disturbance signal, indicating that the consistency of the impedances obtained from multiple tests is better. Thus, it can be seen that in the case of air bubbles in the sample, increasing the disturbance signal can effectively improve the precision of the detected impedance, and thus can effectively improve the detection accuracy of hematocrit.

[0058] The embodiments in this specification are described in a progressive manner. The key point of each embodiment is to illustrate the differences from other embodiments. For the same or similar parts among the various embodiments, reference can be made to each other.

[0059] The above description of the disclosed embodiments enables those skilled in the art to implement or use the present application. Various modifications to these embodiments will be obvious to those skilled in the art. The general principles defined herein can be implemented in other embodiments without departing from the spirit or scope of the present application. Therefore, the present application will not be limited to these embodiments shown herein, but rather will be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A method for detecting hematocrit, characterized in that, Including: After determining that the injection of the blood sample to be tested is completed, continuously apply a plurality of disturbance signals with different frequencies to the HCT electrode within a first preset duration; After an interval of a second preset duration, apply a detection signal to the HCT electrode; Obtain the impedance of the blood sample to be tested; According to the impedance and a preset equation, obtain the hematocrit of the blood sample to be tested.

2. The method according to claim 1, wherein The disturbance signal is a sine-wave AC signal, and the frequency range is 4 Hz to 1 kHz.

3. The method according to claim 2, characterized in that, The plurality of different frequencies of the disturbance signal at least include two frequencies respectively located at both ends of the frequency range.

4. The method according to claim 1, wherein The application duration of the disturbance signal with the highest frequency among the plurality of different frequencies of the disturbance signal is greater than 50% of the first preset duration.

5. The method according to claim 1, characterized in that After determining that the injection of the blood sample to be tested is completed, continuously applying a plurality of disturbance signals with different frequencies to the HCT electrode within a first preset duration includes: After determining that the injection of the blood sample to be tested is completed, continuously apply a disturbance signal with a first preset frequency and a disturbance signal with a second preset frequency to the HCT electrode within a first preset duration, where the first preset frequency is less than the second preset frequency.

6. The method according to claim 5, wherein After determining that the injection of the blood sample to be tested is completed, continuously applying a disturbance signal with a first preset frequency and a disturbance signal with a second preset frequency to the HCT electrode within a first preset duration includes: After determining that the injection of the blood sample to be tested is completed, perform an alternating operation for a first preset number of times. Each alternating operation includes: applying a disturbance signal with a first preset frequency to the HCT electrode for a first preset sub-duration, and then applying a disturbance signal with a second preset frequency to the HCT electrode for a second preset sub-duration; Wherein, the second preset sub-duration is greater than the first preset sub-duration.

7. The method according to claim 6, characterized in that After performing the alternating operation for the first preset number of times, it further includes performing an additional operation. The additional operation includes: applying a disturbance signal with a first preset frequency to the HCT electrode for a third preset sub-duration, and then applying a disturbance signal with a second preset frequency to the HCT electrode for a fourth preset sub-duration; Wherein, the fourth preset sub-duration is greater than the third preset sub-duration.

8. The method according to claim 1, characterized in that, After determining that the injection of the blood sample to be tested is completed, continuously applying a plurality of disturbance signals with different frequencies to the HCT electrode within a first preset duration includes: After determining that the injection of the blood sample to be tested is completed, continuously apply a disturbance signal with a third preset frequency, a disturbance signal with a fourth preset frequency, and a disturbance signal with a fifth preset frequency to the HCT electrode within a first preset duration, where the third preset frequency is less than the fourth preset frequency, and the fourth preset frequency is less than the fifth preset frequency.

9. The method according to claim 8, characterized in that, After determining that the injection of the blood sample to be tested is completed, continuously applying a disturbance signal with a third preset frequency, a disturbance signal with a fourth preset frequency, and a disturbance signal with a fifth preset frequency to the HCT electrode within a first preset duration includes: After determining that the injection of the blood sample to be measured is completed, perform an alternating operation for a second preset number of times. Each time the alternating operation includes: first applying a disturbance signal with a third preset frequency to the HCT electrode for a fifth preset sub-duration, then applying a disturbance signal with a fourth preset frequency to the HCT electrode for a sixth preset sub-duration, and then applying a disturbance signal with a fifth preset frequency to the HCT electrode for a seventh preset sub-duration.

10. The method according to claim 8, characterized in that The value of the fourth preset frequency is close to the middle number between the third preset frequency and the fifth preset frequency.

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