Preparation and application of essential oil composition capable of promoting blood circulation, dredging collaterals, inducing resuscitation and tranquilizing mind

Abstract

The invention provides application of combined use of ligusticum wallichii essential oil and borneol in preparation of drugs for treating cardiovascular and cerebrovascular diseases. The dosage ratio of the ligusticum wallichii essential oil to the borneol is as follows: 0.25-0.75 part of the ligusticum wallichii essential oil and 0.25-0.75 part of the borneol. The invention also provides an essential oil composition for promoting blood circulation, dredging channels, inducing resuscitation and calming nerves. The essential oil composition is prepared from the following raw materials in parts by weight: 0.25-0.75 part of ligusticum wallichii essential oil and 0.25-0.75 part of borneol. The essential oil composition capable of promoting blood circulation, dredging collaterals, inducing resuscitation and calming nerves is suitable for prevention and treatment of diseases and daily health care, and pharmacodynamic tests prove that the raw materials are compatible, so that a synergistic effect can be achieved, and the essential oil composition has remarkable advantages in the aspects of improving symptoms, improving life quality and reducing drug dependence.

Description

Technical Field

[0001] The present invention relates to an essential oil composition for activating blood circulation and dredging meridians, and inducing resuscitation and tranquilizing the mind, and its uses. Background Art

[0002] Qi stagnation, blood stasis, and phlegm dampness are the core pathological manifestations of the disorder of qi, blood, and body fluids metabolism in traditional Chinese medicine theory. The three can not only cause diseases independently, but also often transform and combine with each other, resulting in the obstruction of qi and blood circulation and the obstruction of the clear orifices (such as the brain, heart, eyes, etc.), leading to complex diseases, which is a typical manifestation of the theory of "simultaneous diseases of qi, blood, and body fluids" in traditional Chinese medicine.

[0003] "Activating blood circulation and dredging meridians" and "inducing resuscitation and tranquilizing the mind" are the core treatment methods for "simultaneous diseases of qi, blood, and body fluids". Activating blood circulation regulates qi and blood, and when blood circulates smoothly, phlegm stasis is easily transformed; inducing resuscitation promotes the flow of body fluids, and when the orifices are unobstructed, the qi mechanism is smooth; clearing the clear orifices restores the mind, and when the mind is at peace, qi is smooth and blood is harmonious. Cardiovascular and cerebrovascular diseases such as stroke, coronary heart disease, and vascular dementia; metabolic diseases such as obesity, hyperuricemia, hyperlipidemia, and metabolic encephalopathy; neurological diseases such as vertigo syndrome, epilepsy, and headache; gynecological diseases such as dysmenorrhea and amenorrhea, polycystic ovary syndrome, endometriosis, and menopausal syndrome all belong to the category of simultaneous diseases of qi, blood, and body fluids. "Activating blood circulation and dredging meridians" and "inducing resuscitation and tranquilizing the mind" cooperate with each other to achieve the dual goals of "unobstructed blood vessels and tranquil mind" in acute and chronic diseases of simultaneous diseases of qi, blood, and body fluids, reflecting the wisdom of "treating the symptoms in acute cases and the root cause in chronic cases", "treating the disease by seeking the root cause", and "overall regulation" in traditional Chinese medicine.

[0004] Cardiovascular and cerebrovascular diseases usually adopt a multi-dimensional treatment plan combining drug treatment, surgical intervention, lifestyle adjustment, and rehabilitation management. Drug treatment is the cornerstone of cardiovascular and cerebrovascular disease management, including antiplatelet drugs (such as aspirin, clopidogrel, ticagrelor, etc.), antihypertensive drugs, lipid-lowering drugs (such as statins), anticoagulant drugs (such as warfarin, dabigatran, rivaroxaban, etc.). However, it is prone to drug side effects such as liver and kidney function damage, bleeding risk, and interference with metabolism. The drug interactions are complex, and the long-term compliance of patients is poor.

[0005] Volatile oils of traditional Chinese medicine, also known as essential oils of traditional Chinese medicine, are indispensable active ingredients of aromatic traditional Chinese medicine. They usually have strong pharmacological activities, are mostly aromatic, pungent, dispersing, warm, and dredging. Their molecules are fine, volatile, and lipid-soluble, and are easy to penetrate the body's biological membranes, with high bioavailability, and can be rapidly absorbed and take effect through the gastrointestinal tract, nasal cavity, oral mucosa, skin and other channels. It has the characteristics of rapid action, effectiveness, safety, mildness, not easy to produce drug resistance and dependence, and is suitable for long-term use. Traditional Chinese medicine essential oils can directly and rapidly take effect in cardiovascular and cerebrovascular disease events through functions such as dilating blood vessels, antiplatelet aggregation, protecting vascular endothelium, and penetrating the blood-brain barrier. It can also act on the zang-fu organs and meridians, promote the flow of qi and blood, balance the yin and yang of the body, and regulate the body's functions as a whole, which can not only improve symptoms but also prevent recurrence.

[0006] There are literature reports that Chuanxiong Rhizome and Borneol are the paired herbs of Suxiao Jiuxin Pills (Li Pu, Suxiao Jiuxin Pills and Its New Paired Herbs, Chinese Community Doctors, Issue 7, 2014), and what is disclosed therein is the oral Suxiao Jiuxin Pills.

[0007] CN200510107900.7, Invention Title: A Dropping Pill Preparation for Treating Coronary Heart Disease Angina Pectoris and Its Preparation Method, discloses a new dropping pill preparation for treating coronary heart disease angina pectoris. This dropping pill preparation is prepared from 20.1 parts by weight of Cortex Moutan, 33.6 parts by weight of Chuanxiong Rhizome, and 2.18 parts by weight of Borneol according to the following method: preparing the percolation liquid of Cortex Moutan and recovering ethanol; extracting the volatile oil from Chuanxiong Rhizome with water, decocting the medicinal residues with water, filtering the decoction liquid, combining the filtrate with the above aqueous solution, concentrating into a clear paste, adding ethanol to the clear paste, stirring, standing, sucking the supernatant, and recovering ethanol; combining the medicinal liquids of Cortex Moutan and Chuanxiong Rhizome, and concentrating under reduced pressure to a thick paste; taking polyethylene glycol, heating, and after all melting, adding the above thick paste, the volatile oil of Chuanxiong Rhizome, and Borneol, stirring evenly, dropping from top to bottom into liquid paraffin, draining the formed dropping pills and wiping off the liquid paraffin, then obtaining the product. What this patent document discloses is an oral preparation containing the volatile oil of Chuanxiong Rhizome and Borneol. The steam extraction rate of the volatile oil of Chuanxiong Rhizome is about 0.20% - 0.60%, and when converted to the dosage ratio of the volatile oil of Chuanxiong Rhizome, it is 0.07 - 0.21:2.18, which has a significant difference from the dosage used in this patent.

[0008] Currently, there is no relevant research on the compatibility of the volatile oil of Chuanxiong Rhizome and Borneol for inhalation preparations, and there is no relevant literature report on preparing an inhalation preparation based on the volatile oil of Chuanxiong Rhizome and Borneol for treating coronary heart disease or stroke. Summary of the Invention

[0009] The present invention first combines the volatile oil of Chuanxiong Rhizome and Borneol for inhalation preparations. The present invention also provides an essential oil composition for promoting blood circulation and dredging meridians, and opening orifices and calming the mind.

[0010] The present invention provides the use of the combined use of Chuanxiong essential oil and Borneol in the preparation of an inhalation drug for treating cardiovascular and cerebrovascular diseases. The dosage ratio of the Chuanxiong essential oil and Borneol is: 0.25 - 0.75 parts of Chuanxiong essential oil and 0.25 - 0.75 parts of Borneol.

[0011] Preferably, it is prepared from raw materials with the following weight ratio:

[0012] 0.25 - 0.75 parts of Chuanxiong essential oil and 0.25 - 0.50 parts of Borneol;

[0013] More preferably, it is prepared from raw materials with the following weight ratio:

[0014] 0.50 part of Chuanxiong essential oil and 0.50 part of Borneol

[0015] Among them, the raw materials also contain 0.25 to 0.75 parts of angelica essential oil and musk. The weight ratio of the raw materials is: 0.25 to 0.75 parts of chuanxiong essential oil, 0.25 to 0.75 parts of borneol, 0.25 to 0.75 parts of angelica essential oil, and 0.005 to 0.015 parts of musk.

[0016] Preferably, it is prepared from the raw materials with the following weight ratio:

[0017] 0.50 part of chuanxiong essential oil, 0.50 part of borneol, 0.50 part of angelica essential oil, and 0.01 part of musk

[0018] Among them, the raw materials also contain frankincense essential oil, turmeric essential oil, and acorus tatarinowii essential oil. The weight ratio of the raw materials is:

[0019] 0.25 to 0.75 parts of chuanxiong essential oil, 0.25 to 0.75 parts of borneol, 0.25 to 0.75 parts of angelica essential oil, 0.005 to 0.015 parts of musk, 0.5 to 1.5 parts of frankincense essential oil, 0.5 to 1.5 parts of turmeric essential oil, and 0.5 to 1.5 parts of acorus tatarinowii essential oil

[0020] Preferably, it is prepared from the raw materials with the following weight ratio:

[0021] 0.50 part of chuanxiong essential oil, 0.50 part of borneol, 0.50 part of angelica essential oil, 0.01 part of musk, 1.0 part of frankincense essential oil, 1.0 part of turmeric essential oil, and 1.0 part of acorus tatarinowii essential oil.

[0022] Among them, the raw materials also contain cyperus rotundus essential oil, pogostemon cablin essential oil, and agarwood essential oil. The weight ratio of the raw materials is:

[0023] 0.25 to 0.75 parts of chuanxiong essential oil, 0.25 to 0.75 parts of borneol, 0.25 to 0.75 parts of angelica essential oil, 0.005 to 0.015 parts of musk, 0.5 to 1.5 parts of frankincense essential oil, 0.5 to 1.5 parts of turmeric essential oil, 0.5 to 1.5 parts of acorus tatarinowii essential oil, 0.5 to 1.5 parts of cyperus rotundus essential oil, 0.5 to 1.5 parts of pogostemon cablin essential oil, and 0.25 to 0.75 parts of agarwood essential oil.

[0024] Preferably, it is prepared from the raw materials with the following weight ratio:

[0025] 0.50 part of chuanxiong essential oil, 0.50 part of borneol, 0.50 part of angelica essential oil, 0.01 part of musk, 1.0 part of frankincense essential oil, 1.0 part of turmeric essential oil, 1.0 part of acorus tatarinowii essential oil, 1.0 part of cyperus rotundus essential oil, 1.0 part of pogostemon cablin essential oil, and 0.50 part of agarwood essential oil.

[0026] Among them, the raw materials also contain citrus reticulata essential oil and clove essential oil. The weight ratio of the raw materials is:

[0027] 0.25 - 0.75 parts of Chuanxiong essential oil, 0.25 - 0.75 parts of borneol, 0.005 - 0.015 parts of musk, 0.25 - 0.75 parts of Angelica essential oil, 0.5 - 1.5 parts of frankincense essential oil, 0.5 - 1.5 parts of turmeric essential oil, 0.5 - 1.5 parts of Acorus tatarinowii essential oil, 0.5 - 1.5 parts of Cyperus rotundus essential oil, 0.5 - 1.5 parts of Pogostemon cablin essential oil, 0.25 - 0.75 parts of Aquilaria sinensis essential oil, 1.0 - 3.0 parts of tangerine peel essential oil, 0.5 - 1.5 parts of clove essential oil.

[0028] Preferably, it is prepared from the raw materials with the following weight ratios:

[0029] 0.50 parts of Chuanxiong essential oil, 0.50 parts of borneol, 0.50 parts of Angelica essential oil, 0.01 parts of musk, 1.0 part of frankincense essential oil, 1.0 part of turmeric essential oil, 1.0 part of Acorus tatarinowii essential oil, 1.0 part of Cyperus rotundus essential oil, 1.0 part of Pogostemon cablin essential oil, 0.50 parts of Aquilaria sinensis essential oil, 2.0 parts of tangerine peel essential oil, 1.0 part of clove essential oil.

[0030] The essential oil composition of the present invention uses the above raw materials as active ingredients and is prepared into common inhalation preparations and external preparations by adding acceptable excipients or auxiliary components.

[0031] In the raw material formula of the present invention, Chuanxiong (Rhizoma Chuanxiong) promotes blood circulation and qi movement, dispels wind and relieves pain. It is known as the "qi medicine in blood" and can promote the circulation of qi and blood to dredge the meridians. It is especially good at ascending to the head and descending to the blood sea. "Huahui Yanyu" states that it "ascends to the head, regulates menstruation downward, relieves qi stagnation in the middle, and is the qi medicine in blood... with a pungent taste and yang nature, its qi is dispersive and running without the state of yin coagulation and viscosity. Although it enters the blood aspect, it can also dispel all kinds of wind and regulate all kinds of qi"; Borneol (Borneolum Syntheticum) induces resuscitation and alleviates pain, clears heat and relieves pain, and can open all orifices and disperse stagnant fire; the ability of Chuanxiong to promote blood circulation and qi movement helps Borneol to induce resuscitation and awaken the mind, and the pungent and dispersive nature of Borneol makes the medicinal power more easily reach the meridians and viscera. The two complement each other and are used as the monarch drugs together. Moschus (Moschus) induces resuscitation and awakens the mind, promotes blood circulation, dredges channels and relieves pain. It directly enters the heart meridian to open the orifices and awaken the mind, and also disperses stasis and dredges the meridians. It synergistically enhances the power of opening the orifices with Borneol, and can also prevent the cold nature of Borneol from damaging yang qi. It can not only dredge blood stasis in the meridians but also open the orifices and relieve obstruction. Angelica sinensis (Oliv.) Diels nourishes blood and promotes blood circulation, regulates menstruation and relieves pain, and helps Chuanxiong promote blood circulation without damaging blood. Olibanum (Olibanum) promotes blood circulation and relieves pain, Curcuma longa L. (Rhizoma Curcumae Longae) breaks blood and promotes qi movement, dredges channels and relieves pain, enhancing the power of Chuanxiong to break blood and dredge the meridians; Acorus tatarinowii Schott (Rhizoma Acori Tatarinowii) opens orifices and resolves phlegm, awakens the mind and improves intelligence, promotes digestion by removing dampness, and can also soothe the nerves. It can assist Moschus and Borneol in opening orifices, balance the effect of opening orifices and awakening the mind without damaging yin, and also dispel the pathogenic factors of phlegm and dampness covering the clear orifices. These five herbs are used as ministerial drugs together. Cyperus rotundus L. (Rhizoma Cyperi) soothes the liver and relieves depression, regulates qi and soothes the middle, regulates menstruation and relieves pain, promoting qi movement to assist blood circulation (qi movement promotes blood movement); Aquilaria sinensis (Lour.) Gilg promotes qi movement and relieves pain, warms the kidney and assists in receiving qi, regulating the qi movement in the lower energizer to prevent the ascending and dispersing of blood-activating drugs from causing qi reversal; Pogostemon cablin (Blanco) Benth. (Herba Pogostemonis) aromatically resolves turbidity, harmonizes the stomach and stops vomiting, can dispel pathogenic factors of dampness and turbidity, and assist Acorus tatarinowii Schott in resolving phlegm and dampness covering the orifices; Citrus reticulata Blanco (Pericarpium Citri Reticulatae) regulates qi and strengthens the spleen, dries dampness and resolves phlegm, not only assisting Cyperus rotundus L. in regulating qi but also addressing the source of phlegm and dampness, and regulating the qi movement in the middle energizer. Flos Caryophylli (Flos Caryophylli) warms the middle and reduces adverse qi, tonifies the kidney and assists yang, preventing the cold nature of Borneol from injuring the stomach. These five herbs are used as adjuvant drugs together. Borneol also serves as the guiding drug, and its pungent and dispersive nature can lead all the herbs to ascend to the head and directly reach the heart orifices. The whole formula takes promoting blood circulation and dredging the meridians, promoting qi movement and relieving pain, opening orifices and calming the mind as the core, taking into account regulating qi and resolving dampness, embodying the therapeutic principles of "treating qi and blood simultaneously", "treating phlegm and stasis simultaneously", and "opening orifices to calm the mind" in traditional Chinese medicine. It can be used for diseases related to qi stagnation and blood stasis, and obstruction of the meridians and collaterals such as cardiovascular and cerebrovascular diseases, dysmenorrhea, and traumatic blood stasis, and can also be used for syndromes of phlegm-dampness, heat-toxin, qi stagnation, etc. covering the clear orifices or disturbing the mind, such as stroke coma, dementia, and abnormal mental states.

[0032] An essential oil composition for promoting blood circulation and dredging the meridians, and opening orifices and calming the mind provided by the present invention is applicable to the prevention and treatment of diseases and daily health care. Pharmacodynamic experiments prove that the compatibility of the raw material formula can play a synergistic effect and has significant advantages in improving symptoms, enhancing the quality of life, and reducing drug dependence. Detailed implementation mode

[0033] Example 1 Preparation of the essential oil composition of the present invention

[0034] Example 1: 0.5 part of Chuanxiong essential oil, 0.5 part of Borneol

[0035] Example 2: 0.25 part of Chuanxiong essential oil, 0.5 part of Borneol

[0036] Example 3: 0.75 part of Chuanxiong essential oil, 0.25 part of Borneol

[0037] Example 4: 0.50 parts of Chuanxiong essential oil, 0.50 parts of borneol, 0.50 parts of Angelica essential oil, 0.01 parts of musk

[0038] Example 5: 0.50 parts of Chuanxiong essential oil, 0.50 parts of borneol, 0.75 parts of Angelica essential oil, 0.005 parts of musk

[0039] Example 6: 0.50 parts of Chuanxiong essential oil, 0.50 parts of borneol, 0.25 parts of Angelica essential oil, 0.015 parts of musk

[0040] Example 7: 0.50 parts of Chuanxiong essential oil, 0.50 parts of borneol, 0.50 parts of Angelica essential oil, 0.01 parts of musk, 1.0 part of frankincense essential oil, 1.0 part of turmeric essential oil, 1.0 part of Acorus tatarinowii essential oil

[0041] Example 8: 0.50 parts of Chuanxiong essential oil, 0.50 parts of borneol, 0.50 parts of Angelica essential oil, 0.01 parts of musk, 0.5 part of frankincense essential oil, 0.5 part of turmeric essential oil, 1.5 parts of Acorus tatarinowii essential oil

[0042] Example 9: 0.50 parts of Chuanxiong essential oil, 0.50 parts of borneol, 0.50 parts of Angelica essential oil, 0.01 parts of musk, 1.5 parts of frankincense essential oil, 1.5 parts of turmeric essential oil, 0.5 part of Acorus tatarinowii essential oil

[0043] Example 10: 0.50 parts of Chuanxiong essential oil, 0.50 parts of borneol, 0.50 parts of Angelica essential oil, 0.01 parts of musk, 1.0 part of frankincense essential oil, 1.0 part of turmeric essential oil, 1.0 part of Acorus tatarinowii essential oil, 1.0 part of Cyperus rotundus essential oil, 1.0 part of Pogostemon cablin essential oil, 0.50 part of Aquilaria sinensis essential oil

[0044] Example 11: 0.50 parts of Chuanxiong essential oil, 0.50 parts of borneol, 0.50 parts of Angelica essential oil, 0.01 parts of musk, 1.0 part of frankincense essential oil, 1.0 part of turmeric essential oil, 1.0 part of Acorus tatarinowii essential oil, 0.5 part of Cyperus rotundus essential oil, 0.5 part of Pogostemon cablin essential oil, 0.25 part of Aquilaria sinensis essential oil

[0045] Example 12: 0.50 parts of Chuanxiong essential oil, 0.50 parts of borneol, 0.50 parts of Angelica essential oil, 0.01 parts of musk, 1.0 part of frankincense essential oil, 1.0 part of turmeric essential oil, 1.0 part of Acorus tatarinowii essential oil, 1.5 parts of Cyperus rotundus essential oil, 1.5 parts of Pogostemon cablin essential oil, 0.50 part of Aquilaria sinensis essential oil

[0046] Example 13: 0.50 parts of Chuanxiong essential oil, 0.50 parts of borneol, 0.50 parts of Angelica essential oil, 0.01 parts of musk, 1.0 part of frankincense essential oil, 1.0 part of turmeric essential oil, 1.0 part of Acorus tatarinowii essential oil, 1.0 part of Cyperus rotundus essential oil, 1.0 part of Pogostemon cablin essential oil, 0.50 part of Aquilaria sinensis essential oil, 2.0 parts of Citrus reticulata Blanco essential oil, 1.0 part of Eugenia caryophyllata essential oil

[0047] Example 14: 0.50 parts of Chuanxiong essential oil, 0.50 parts of borneol, 0.50 parts of Angelica essential oil, 0.01 parts of musk, 1.0 part of frankincense essential oil, 1.0 part of turmeric essential oil, 1.0 part of Acorus tatarinowii essential oil, 1.0 part of Cyperus rotundus essential oil, 1.0 part of Pogostemon cablin essential oil, 0.50 part of Aquilaria sinensis essential oil, 1.0 part of Citrus reticulata Blanco essential oil, 1.5 parts of clove essential oil

[0048] Example 15: 0.50 parts of Chuanxiong essential oil, 0.50 parts of borneol, 0.50 parts of Angelica essential oil, 0.01 parts of musk, 1.0 part of frankincense essential oil, 1.0 part of turmeric essential oil, 1.0 part of Acorus tatarinowii essential oil, 1.0 part of Cyperus rotundus essential oil, 1.0 part of Pogostemon cablin essential oil, 0.50 part of Aquilaria sinensis essential oil, 3.0 parts of Citrus reticulata Blanco essential oil, 0.5 part of clove essential oil

[0049] The above essential oil raw materials are extracted by extraction methods such as steam distillation method, molecular distillation method, supercritical CO2 extraction method, etc. to obtain single essential oils or purchase commercially available single essential oil products.

[0050] In the above examples, the turmeric and Chuanxiong essential oils are extracted by steam distillation method of the whole plant. The remaining single essential oils are extracted by steam distillation method or supercritical CO2 extraction method using the medicinal parts.

[0051] Pre - test of the efficacy of Example 16

[0052] In the pre - test of efficacy, the compatibility ratio of essential oils was screened for its effect on the rat model of ischemic stroke. The compound essential oil can reduce the neurological function score and the ratio of cerebral infarction volume of the model rats. The effect of Example 10 is the best, followed by Example 13. The odor acceptability of Examples 13, 14, and 15 is the best.

[0053] The beneficial effects of the present invention are demonstrated by the following pharmacodynamic experiments.

[0054] Experimental example 1 Effect on rats with ischemic stroke

[0055] 1. Establish a rat model of ischemic stroke

[0056] The Longa suture method was used to establish a rat model of ischemic stroke. The rats were anesthetized, and the left common carotid artery, external carotid artery, and internal carotid artery were exposed. The distal end of the external carotid artery and the proximal end of the common carotid artery were ligated, and the internal carotid artery was temporarily blocked. A nylon suture about 0.25 mm in diameter was inserted into the blood vessel through a puncture in the external carotid artery and reached the middle cerebral artery through the internal carotid artery. After 2 hours, the suture was withdrawn to restore blood supply and the port was ligated, and the wound was sutured. The rats were placed under a heating lamp to maintain body temperature until they regained consciousness. At 72 hours after surgery, the Zea-Longa score was used to evaluate the neurological function of the rats. A neurological function score of 1-3 indicated successful modeling. 0 points: no symptoms of nerve damage, normal movement, and no abnormal phenomena; 1 point: the left forelimb was flexed and could not be extended normally when the tail was lifted; 2 points: a circular walking phenomenon occurred; 3 points: difficulty walking and the body tilted to the left; 4 points: unable to walk spontaneously or in a coma.

[0057] 2 Experimental grouping, administration, and modeling

[0058] One hundred SPF-grade male SD rats, weighing 200±20 g, were adaptively raised in the laboratory for 5 days. The above method was used to replicate the rat model of ischemic stroke. Seventy-four rats were successfully modeled and randomly divided into a model group, a single essential oil group of Ligusticum chuanxiong, compound essential oil group 1 (Ligusticum chuanxiong - borneol 50:50), compound essential oil group 2 (Ligusticum chuanxiong - borneol - Angelica sinensis - Moschus 50:50:50:1), compound essential oil group 3 (Ligusticum chuanxiong - borneol - Angelica sinensis - Moschus - Boswellia carterii - Curcuma longa - Acorus tatarinowii 50:50:50:1:100:100:100), compound essential oil group 4 (Ligusticum chuanxiong - borneol - Angelica sinensis - Moschus - Boswellia carterii - Curcuma longa - Acorus tatarinowii - Pogostemon cablin - Aquilaria sinensis 50:50:50:1:100:100:100:100:50), compound essential oil group 5 (Ligusticum chuanxiong - borneol - Angelica sinensis - Moschus - Boswellia carterii - Curcuma longa - Acorus tatarinowii - Pogostemon cablin - Aquilaria sinensis - Citrus reticulata Blanco - Syzygium aromaticum 50:50:50:1:100:100:100:100:50:200:100), and a positive drug group of butylphthalide, with 9 rats in each group and 2 remaining. Another 12 rats were only given sham surgery without suture embolization and ligation, and the remaining operations were the same as those in the model group, serving as the sham surgery group, and 10 rats survived in the sham surgery group.

[0059] The rats in the sham surgery group, model group, and essential oil group were subjected to atomized aromatherapy for 30 minutes. The sham surgery group and the model group used 50 ml of water, and the essential oil group had about 0.2 g of essential oil dropped into 50 ml of water. The space volume of the animal aromatherapy chamber was about 50 L (52 cm×38 cm×25 cm), and the administration dose was 0.10 g / kg. The butylphthalide group was administered by gavage at a dose of 60 mg / kg, and the administration was continuous for 14 days.

[0060] 3 Effects on neurological function score, cerebral infarction volume ratio, and neuronal apoptosis

[0061] At 60 min after the last administration in each group, the neurological function of rats was evaluated using the Zea-Longa score.

[0062] The cerebral infarction volume of rats was detected by TTC staining. After the neurological function score was completed, the rats were anesthetized with sodium pentobarbital, sacrificed after blood collection from the abdominal aorta. Three rats were randomly selected from each group. After removing the cerebellum, brainstem and olfactory bulb of the rat brain, it was stored in a -4°C refrigerator for 30 min, and then the rat brain tissue was cut into 2-mm thick slices. The brain slices were stained with 2% TTC solution and incubated in a 37°C constant temperature water bath in the dark for 20 min. During this period, the brain slices were turned over every 5 min to ensure uniform staining of the brain slices. The pale area was the infarction area, and the red was the non-infarction area. After taking pictures, the cerebral infarction volume was measured using Image J image analysis software. The percentage of cerebral infarction volume = (infarction volume / total brain volume) × 100%.

[0063] Neuronal apoptosis was detected by the TUNEL method. The infarcted side brain tissues of the remaining 6 rats in each group were taken (the same side was taken in the sham operation group), fixed with 4% paraformaldehyde, embedded in paraffin and cut into 5-μm slices, and then dewaxed and operated according to the instructions. Under the microscope, the TUNEL-positive cells showed yellowish-brown or brownish-black granules. Calculate the neuronal apoptosis index: the number of positive cells / the total number of cells × 100%.

[0064] Effects on the levels of serum CAT, NGF, and VEGF

[0065] For the blood from the abdominal aorta, serum was obtained by centrifugation. The operation was carried out according to the steps of the kit instructions. The level of CAT (catalase) was detected by the colorimetric method, and the levels of NGF (nerve growth factor) and VEGF (vascular endothelial growth factor) were detected by the Elisa method.

[0066] 5. Experimental results

[0067] 5.1 Effects on neurological function score, cerebral infarction volume ratio, and neuronal apoptosis

[0068] Compared with the sham operation group, the neurological function score, cerebral infarction volume ratio, and percentage of neuronal apoptosis in the model group rats were all significantly increased (P < 0.05); compared with the model group, the neurological function scores of the rats in the compound essential oil group and the positive butylphthalide group were significantly decreased, and the cerebral infarction volume ratio and the percentage of neuronal apoptosis in the essential oil group and the positive butylphthalide group were significantly decreased (P < 0.05); there was a significant difference in the effect between compound essential oil 1 and the single essential oil of Ligusticum chuanxiong, which proved that the combination of the essential oil of Ligusticum chuanxiong and borneol in this application could play a synergistic effect; compared with the positive butylphthalide group, the neurological function score of the rats in the compound essential oil 4 group was significantly decreased (P < 0.05), the cerebral infarction volume ratio in the compound essential oil 3 group and the compound essential oil 4 group was significantly decreased (P < 0.05), and the percentage of neuronal apoptosis in the compound essential oil 2 group, the compound essential oil 3 group, and the compound essential oil 4 group was significantly decreased (P < 0.05); the effect of compound essential oil 4 was the best. The results are shown in Table 1.

[0069] Table 1 Effects on neurological function score, cerebral infarction volume ratio, and neuronal apoptosis in rats with ischemic stroke

[0070]

[0071] Compared with the model group, * P < 0.05, ** P < 0.01; compared with the sham operation group, # P < 0.05, ## P < 0.01;

[0072] Compared with the positive drug butylphthalide group, & P < 0.05, && P < 0.01.

[0073] 5.2 Effects on serum CAT, NGF, and VEGF levels

[0074] Compared with the sham operation group, the contents of serum CAT and NGF in the model group rats were significantly decreased, and there was a tendency for the content of serum VEGF in the rats to increase (P < 0.05); compared with the model group, the contents of serum CAT and NGF in the essential oil group and the positive butylphthalide group rats were significantly increased, and the content of VEGF in the compound essential oil group was significantly increased (P < 0.05); there was a significant difference in the effect between compound essential oil 1 and the single essential oil of Ligusticum chuanxiong, which proved that the combination of the essential oil of Ligusticum chuanxiong and borneol in this application could play a synergistic effect; compared with the positive butylphthalide group, the serum CAT content in the compound essential oil 2 group, the compound essential oil 3 group, the compound essential oil 4 group, and the compound essential oil 5 group rats was significantly increased, the serum NGF content in the compound essential oil 4 group was significantly increased, and the serum VEGF content in the compound essential oil 3 group, the compound essential oil 4 group, and the compound essential oil 5 group rats was significantly increased (P < 0.05); the effect of compound essential oil 4 was the best. The results are shown in Table 2.

[0075] Effect of Table 2 on Serum CAT, NGF, and VEGF Levels in Rats with Ischemic Stroke

[0076]

[0077]

[0078] Compared with the model group, * P < 0.05, ** P < 0.01; compared with the sham operation group, # P < 0.05, ## P < 0.01;

[0079] Compared with the positive drug butylphthalide group, & P < 0.05, && P < 0.01.

[0080] Experimental Example 2, Effect on Rats with Coronary Heart Disease

[0081] 1. Establish a rat model of coronary heart disease

[0082] A rat model of coronary heart disease was established by a high-fat diet combined with intraperitoneal injection of pituitrin. Rats were fed a high-fat diet (3% cholesterol, 5% white sugar, 20% lard, 1% vitamin D, 2% egg yolk powder, 0.2% propylthiouracil, and 69% basal diet) for 6 weeks, and 30 U / (kg·d) of pituitrin was continuously injected intraperitoneally for the last 3 days. Electrocardiogram detection was performed to confirm the establishment of the rat model of coronary heart disease.

[0083] 2. Experimental grouping, model establishment, and drug administration

[0084] 100 SPF-grade male SD rats, weighing 200 ± 20 g, were adaptively raised in the laboratory for 5 days. Randomly select 10 rats as the blank group and only feed them with basal diet. The remaining rats were modeled according to the modeling method. After the modeling, electrocardiogram detection was performed, and 80 rats were successfully modeled.

[0085] The successfully modeled rats were randomly divided into a model group, a single essential oil group of Ligusticum chuanxiong, compound essential oil group 1 (Ligusticum chuanxiong - borneol 50:50), compound essential oil group 2 (Ligusticum chuanxiong - borneol - Angelica sinensis - Moschus 50:50:50:1), compound essential oil group 3 (Ligusticum chuanxiong - borneol - Angelica sinensis - Moschus - Boswellia carterii - Curcuma longa - Acorus tatarinowii 50:50:50:1:100:100:100), compound essential oil group 4 (Ligusticum chuanxiong - borneol - Angelica sinensis - Moschus - Boswellia carterii - Curcuma longa - Acorus tatarinowii - Pogostemon cablin - Aquilaria sinensis 50:50:50:1:100:100:100:100:50), compound essential oil group 5 (Ligusticum chuanxiong - borneol - Angelica sinensis - Moschus - Boswellia carterii - Curcuma longa - Acorus tatarinowii - Pogostemon cablin - Aquilaria sinensis - Citrus reticulata Blanco - Syzygium aromaticum 50:50:50:1:100:100:100:100:50:200:100), and a positive drug simvastatin group, with 10 rats in each group.

[0086] The rats in the blank group, model group, and essential oil groups were subjected to atomized aromatherapy for 30 min. The blank group and model group used 50 ml of water, and about 0.2 g of essential oil was dropped into 50 ml of water in the essential oil groups. The space volume of the animal aromatherapy chamber was approximately 50 L (52 cm × 38 cm × 25 cm). The administration dose was 0.10 g / kg. The simvastatin group was given intragastric administration at a dose of 2.0 mg / kg and administered continuously for 4 weeks. During the administration period, the rats in the model group, positive group, and essential oil groups continued to be fed with a high-fat diet, while the blank group was fed with a basal diet.

[0087] Effect on the cardiac function of 3 pairs of rats

[0088] Sixty minutes after the last administration of each group, a small animal color Doppler ultrasound imaging system was used to detect cardiac function, and the left ventricular ejection fraction (LVEF), left ventricular end-diastolic dimension (LVIDd), and left ventricular end-systolic dimension (LVIDs) were measured. The data were continuously recorded 3 times, and the average value was taken as the test result.

[0089] Effect on the levels of serum TC, TG, HDL-C, LDL-C, and TXB2 in rats

[0090] Blood was collected from the abdominal aorta of the rats, and the serum was obtained by centrifugation. According to the steps in the kit instructions, a colorimetric method was used to measure the contents of serum TC (total cholesterol), TG (triglyceride), HDL-C (high-density lipoprotein cholesterol), and LDL-C (low-density lipoprotein cholesterol); an ELISA method was used to measure the content of serum TXB2 (thromboxane B2).

[0091] 5 Experimental results

[0092] 5.1 Effects on the cardiac function of rats

[0093] Compared with the normal group, the left ventricular ejection fraction (LVEF) of rats in the model group decreased significantly, while the left ventricular end-diastolic diameter (LVIDd) and left ventricular end-systolic diameter (LVIDs) increased significantly (P < 0.05); compared with the model group, the LVEF of rats in the essential oil group and the positive simvastatin group increased significantly, and the LVIDd and LVIDs decreased significantly (P < 0.05); the effect of compound essential oil 1 was significantly different from that of the single Ligusticum chuanxiong essential oil, indicating that the combination of Ligusticum chuanxiong essential oil and borneol in this application can play a synergistic effect; compared with the positive group, the LVEF of rats in the compound essential oil 2 group, compound essential oil 3 group, and compound essential oil 4 group increased significantly, and the LVIDd and LVIDs decreased significantly (P < 0.05). The effect of compound essential oil 3 was the best. The results are shown in Table 3.

[0094] Table 3 Effects on the cardiac function of rats with coronary heart disease

[0095]

[0096] Compared with the model group, * P < 0.05, ** P < 0.01; compared with the blank group, # P < 0.05, ## P < 0.01;

[0097] Compared with the positive drug simvastatin group, & P < 0.05, && P < 0.01.

[0098] 5.2 Effects on the levels of serum TC, TG, HDL-C, LDL-C, and TXB2 in rats

[0099] Compared with the normal group, the levels of serum TC, TG, LDL-C, and TXB2 in rats in the model group increased significantly, while the level of serum HDL-C decreased significantly (P < 0.05); compared with the model group, the levels of serum TC, TG, LDL-C, and TXB2 in the essential oil group and the positive simvastatin group decreased significantly, and the level of serum HDL-C increased significantly (P < 0.05); the effect of compound essential oil 1 was significantly different from that of the single Ligusticum chuanxiong essential oil, indicating that the combination of Ligusticum chuanxiong essential oil and borneol in this application can play a synergistic effect; compared with the positive group, the levels of serum TG, LDL-C, and TXB2 in the compound essential oil 3 group, compound essential oil 4 group, and compound essential oil 5 group decreased significantly, and the level of serum HDL-C increased significantly. The level of serum TC in the compound essential oil 3 group decreased significantly (P < 0.05). The effect of compound essential oil 3 was the best. The results are shown in Table 4.

[0100] Effect of Table 4 on Serum TC, TG, HDL-C, LDL-C, and TXB2 Levels in Coronary Heart Disease Rats

[0101]

[0102] Compared with the model group, * P < 0.05, ** P < 0.01; compared with the blank group, # P < 0.05, ## P < 0.01;

[0103] Compared with the positive drug simvastatin group, & P < 0.05, && P < 0.01.

[0104] Compound essential oil has a regulatory effect on dyslipidemia and a protective effect on the cardiovascular system by reducing the content of TXB2 and increasing the content of CAT and NGF.

[0105] Test Example 3, Clinical Efficacy

[0106] 1 Therapeutic Effect on Hemiplegia of Ischemic Stroke

[0107] General Information: Sixty-eight patients with hemiplegia of ischemic stroke admitted to a certain hospital in Chengdu from March 2023 to September 2024 were selected. There were 40 males and 28 females; the age ranged from 43 to 75 years old, with an average age of 61.45 ± 7.49 years; the course of disease was 4 to 26 weeks; the paralyzed side: 38 cases on the left side and 30 cases on the right side; 16 cases were complicated with hypertension, 10 cases with diabetes, and 8 cases with coronary heart disease. They were randomly divided into two groups for a prospective controlled study. There were no statistically significant differences in gender, age, course of disease, past medical history, etc. between the two groups of patients (P > 0.05), and they were comparable.

[0108] 1.1 Inclusion Criteria

[0109] The diagnostic criteria for hemiplegia of ischemic stroke were formulated with reference to "Diagnostic Guidelines for Various Major Cerebrovascular Diseases in China 2019", "Chinese Guidelines for the Diagnosis and Treatment of Acute Ischemic Stroke 2018", and "Clinical Practice Guidelines - Neurology Volume";

[0110] (1) Confirmed as ischemic stroke by CT / MRI examination;

[0111] (2) First onset, and the condition is in the recovery period;

[0112] (3) With unilateral limb sensory and functional disorders;

[0113] (4) Stable vital signs.

[0114] (5) Voluntarily signed the informed consent form.

[0115] 1.2 Exclusion criteria

[0116] (1) In the acute attack phase;

[0117] (2) Those with cerebral hemorrhage;

[0118] (3) Those with acute myocardial infarction or malignant tumors;

[0119] (4) Complicated with dysfunction of important organs such as lungs, liver, and kidneys;

[0120] (5) Complicated with hematological diseases;

[0121] (6) History of mental system diseases, drug or alcohol dependence.

[0122] 1.3 Administration method

[0123] Both the control group and the experimental group were given basic treatments such as antihypertensive, hypoglycemic, antiplatelet aggregation, improving microcirculation, and nourishing nerves, as well as rehabilitation training. On the basis of the control group, the experimental group was given atomized aromatherapy to inhale and sniff compound essential oil 4, once in the morning and once in the evening, 0.50 g each time, for about 30 minutes. The treatment was continued for 8 weeks.

[0124] 1.4 Efficacy evaluation

[0125] The National Institutes of Health Stroke Scale (NIHSS) was used to evaluate the degree of neurological deficit before and after treatment. NIHSS includes 15 dimensions such as cognition, perception, and upper and lower limb movement, with a total score of 42 points. The higher the score, the more severe the neurological impairment. The curative effect was judged according to the reduction rate of NIHSS score. ① Recovery: The patient was conscious, and the limb function was basically normal, with a reduction rate of NIHSS score ≥ 90%; ② Marked effect: The patient was conscious, and the limb function was significantly improved, with 45% ≤ reduction rate of NIHSS score < 90%. ③ Effective: Conscious, with improved limb function, 18% ≤ reduction rate of NIHSS score < 45%; ④ Ineffective: Reduction rate of NIHSS score < 18%

[0126] Reduction rate of NIHSS score = (NIHSS score before treatment - NIHSS score after treatment) / NIHSS score before treatment × 100%.

[0127] Total effective rate = (number of cases of recovery + marked effect + effective) / total number of cases × 100%.

[0128] 2. Therapeutic effect on hemiplegia of ischemic stroke

[0129] Compared with the control group, the cure rate, marked effective rate, effective rate and total effective rate of the experimental group were all higher than those of the control group. The compound essential oil can improve the effective rate of conventional treatment for hemiplegia caused by ischemic stroke. The results are shown in Table 5.

[0130] Table 5 Comparison of clinical efficacy of patients with hemiplegia caused by ischemic stroke

[0131]

[0132] In summary, the composition of the present invention can improve the symptoms of cardiovascular diseases such as coronary heart disease and stroke, with rapid and obvious effects. The compounding of various essential oils can play a synergistic effect, and different ratios can be targeted at different adaptabilities, and can be used for both the prevention and treatment of diseases and the rehabilitation of diseases.

Claims

1. Use of combined ligusticum wallichii essential oil and borneol in the preparation of an inhalation drug for treating cardiovascular and cerebrovascular diseases, wherein the dosage ratio of ligusticum wallichii essential oil to borneol is as follows: Ligusticum wallichii essential oil 0.25 - 0.75 parts, borneol 0.25 - 0.75 parts.

2. The use according to claim 1, wherein: The dosage ratio of ligusticum wallichii essential oil to borneol is as follows: Ligusticum wallichii essential oil 0.5 parts, borneol 0.5 parts.

3. The use according to claim 1 or 2, characterized in that: The drug is for treating coronary heart disease and stroke.

4. An essential oil composition for activating blood circulation and dredging channels, and opening orifices and tranquilizing the mind, characterized in that: It is prepared from raw materials with the following weight ratios: Ligusticum wallichii essential oil 0.25 - 0.75 parts, borneol 0.25 - 0.75 parts.

5. The essential oil composition for activating blood circulation and dredging channels, and inducing resuscitation and tranquilizing the mind according to claim 4, wherein: It also contains angelica essential oil and musk, and the weight ratio is: ligusticum wallichii essential oil 0.25 - 0.75 parts, borneol 0.25 - 0.75 parts, angelica essential oil 0.25 - 0.75 parts, musk 0.005 - 0.015 parts. Preferably, it is prepared from raw materials with the following weight ratios: ligusticum wallichii essential oil 0.50 parts, borneol 0.50 parts, angelica essential oil 0.50 parts, musk 0.01 parts.

6. The essential oil composition for activating blood circulation and dredging channels, and inducing resuscitation and tranquilizing the mind according to claim 5, characterized in that: It also contains frankincense essential oil, turmeric essential oil, and acorus tatarinowii essential oil, and the weight ratio is: ligusticum wallichii essential oil 0.25 - 0.75 parts, borneol 0.25 - 0.75 parts, angelica essential oil 0.25 - 0.75 parts, musk 0.005 - 0.015 parts, frankincense essential oil 0.5 - 1.5 parts, turmeric essential oil 0.5 - 1.5 parts, acorus tatarinowii essential oil 0.5 - 1.5 parts. Preferably, it is prepared from raw materials with the following weight ratios: ligusticum wallichii essential oil 0.50 parts, borneol 0.50 parts, angelica essential oil 0.50 parts, musk 0.01 parts, frankincense essential oil 1.0 parts, turmeric essential oil 1.0 parts, acorus tatarinowii essential oil 1.0 parts.

7. The essential oil composition for activating blood circulation and dredging channels, and inducing resuscitation and tranquilizing the mind according to claim 6, wherein: It also contains cyperus rotundus essential oil, pogostemon cablin essential oil, and aquilaria sinensis essential oil, and the weight ratio is: ligusticum wallichii essential oil 0.25 - 0.75 parts, borneol 0.25 - 0.75 parts, angelica essential oil 0.25 - 0.75 parts, musk 0.005 - 0.015 parts, frankincense essential oil 0.5 - 1.5 parts, turmeric essential oil 0.5 - 1.5 parts, acorus tatarinowii essential oil 0.5 - 1.5 parts. Cyperus rotundus essential oil 0.5 - 1.5 parts, pogostemon cablin essential oil 0.5 - 1.5 parts, aquilaria sinensis essential oil 0.5 - 1.5 parts. Preferably, it is prepared from raw materials with the following weight ratios: ligusticum wallichii essential oil 0.50 parts, borneol 0.50 parts, angelica essential oil 0.50 parts, musk 0.01 parts, frankincense essential oil 1.0 parts, turmeric essential oil 1.0 parts, acorus tatarinowii essential oil 1.0 parts, cyperus rotundus essential oil 1.0 parts, pogostemon cablin essential oil 1.0 parts, aquilaria sinensis essential oil 0.50 parts.

8. The essential oil composition for activating blood circulation and dredging meridians, and inducing resuscitation and tranquilizing the mind according to claim 7, wherein: It also contains citrus reticulata essential oil and clove essential oil, and the weight ratio is: ligusticum wallichii essential oil 0.25 - 0.75 parts, borneol 0.25 - 0.75 parts, angelica essential oil 0.25 - 0.75 parts, musk 0.005 - 0.015 parts, frankincense essential oil 0.5 - 1.5 parts, turmeric essential oil 0.5 - 1.5 parts, acorus tatarinowii essential oil 0.5 - 1.5 parts. Cyperus rotundus essential oil 0.5 - 1.5 parts, pogostemon cablin essential oil 0.5 - 1.5 parts, aquilaria sinensis essential oil 0.5 - 1.5 parts, citrus reticulata essential oil 1.0 - 3.0 parts, clove essential oil 0.5 - 1.5 parts. Preferably, it is prepared from the following raw materials in the following weight ratios: 0.50 part of Chuanxiong essential oil, 0.50 part of borneol, 0.50 part of Angelica essential oil, 0.01 part of musk, 1.0 part of frankincense essential oil, 1.0 part of turmeric essential oil, 1.0 part of Acorus tatarinowii essential oil, 1.0 part of Cyperus rotundus essential oil, 1.0 part of Pogostemon cablin essential oil, 0.50 part of Aquilaria sinensis essential oil, 2.0 parts of Citrus reticulata Blanco essential oil, 1.0 part of Eugenia caryophyllata essential oil.

9. Use of the essential oil composition according to any one of claims 4 - 8 in the preparation of a medicament for treating cardiovascular diseases, wherein the medicament required includes inhalation preparations and external preparations.

10. The use according to claim 9, characterized in that Use of the essential oil composition in an inhalation medicament for treating coronary heart disease or stroke.

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