Anti-infection ointment capable of removing necrotic tissues and promoting granulation and preparation method of anti-infection ointment

Abstract

The invention relates to a necrotic tissue-removing granulation-promoting anti-infection ointment and a preparation method thereof, and belongs to the technical field of traditional Chinese medicines. According to the invention, garden burnet, coptis chinensis, golden cypress, scutellaria baicalensis, polygonum cuspidatum, lithospermum erythrorhizon, borneol, beewax and sesame oil are used as basic raw materials to prepare the necrotic tissue-removing, granulation-promoting and anti-infection ointment; the sanguisorba officinalis extract is prepared into pH response nanoparticles, and the lithospermum erythrorhizon extract is prepared into a lithospermum erythrorhizon extract-lipoic acid compound, so that active substances are released by targeting necrotic tissues, natural removal of the necrotic tissues is promoted, and meanwhile, healthy tissues are protected.

Description

Technical Field

[0001] The present invention belongs to the technical field of traditional Chinese medicine, and relates to an anti-corrosion and myogenic anti-infection ointment and a preparation method thereof. Background Art

[0002] Traditional Chinese medicine anti-corrosion and myogenic preparations take clearing heat and detoxifying, promoting granulation and promoting tissue regeneration as the core concept. They often use Coptis chinensis, Phellodendron amurense, and Scutellaria baicalensis as the antibacterial basis, supplemented with oil components such as beeswax and sesame oil to seal the wound surface, and promote tissue autolysis and regeneration through passive moisturization. However, its mechanism of action has significant defects: low debridement efficiency: relying on the natural secretion of the body's autolytic enzymes, the necrosis tissue clearance period is as long as several weeks, and it is easy to cause the spread of infection; insufficient component targeting: broad-spectrum antibacterial components act on wound surface microorganisms and healthy tissues without distinction, resulting in excessive penetration of drugs in healthy tissues.

[0003] To improve the debridement efficiency, exogenous enzyme preparations (such as collagenase ointment, trypsin gel) have been introduced into clinical practice, and the wound surface is purified by directly decomposing necrotic collagen. However, its application faces multiple challenges: stability and cost issues: enzyme proteins are easily affected by the pH fluctuation of wound exudate and degraded by microbial proteases, requiring cold chain storage and transportation and having a short half-life, with high treatment costs; exogenous enzymes cannot distinguish between necrotic and healthy collagen, and excessive decomposition leads to damage to the wound basement membrane.

[0004] Although in recent years, there have been studies attempting to integrate nanotechnology into traditional Chinese medicine preparations, most of them remain in the stage of single-component coating or simple compounding, and have not broken through the limitations of traditional compatibility thinking. CN118873719A discloses a preparation method of a lithospermum oil electrospun nanofilm for treating burns and scalds, only loading shikonin as an independent active ingredient onto the carrier, without constructing a signal pathway cascade between components, and without involving necrosis tissue targeting or healthy tissue protection. Summary of the Invention

[0005] The purpose of the present invention is to provide an anti-corrosion and myogenic anti-infection ointment and a preparation method thereof, which can promote the natural removal of necrotic tissue and protect healthy tissues during medication.

[0006] The purpose of the present invention can be achieved by the following technical solutions:

[0007] An anti-corrosion and myogenic anti-infection ointment, the raw materials include:

[0008] Antibacterial and anti-inflammatory base material: 8-12 parts by weight of Coptis chinensis, 8-12 parts by weight of Phellodendron amurense, 8-12 parts by weight of Scutellaria baicalensis, 6-10 parts by weight of Polygonum cuspidatum;

[0009] Transdermal penetration enhancer: 1-3 parts by weight of borneol;

[0010] Thermosensitive matrix: 20-30 parts by weight of beeswax, 25-35 parts by weight of sesame oil;

[0011] Targeted release extractant: 5-8 parts by weight of Sanguisorba officinalis extract pH-responsive nanoparticles, 4-6 parts by weight of Lithospermum erythrorhizon extract-lipoic acid complex.

[0012] As a preferred technical solution of the present invention, the preparation method of the Sanguisorba officinalis extract pH-responsive nanoparticles is as follows:

[0013] A1. Dissolve Sanguisorba officinalis powder in 60-80% ethanol solution, perform gradient elution through an AB-8 macroporous resin column, collect the Sanguisorba saponin enrichment section and dry it to obtain Sanguisorba officinalis extract;

[0014] A2. Dissolve quaternary ammonium chitosan in 0.8-1.2% acetic acid solution to prepare a solution with a quaternary ammonium chitosan concentration of 1-3 mg / mL;

[0015] A3. Dissolve Sanguisorba officinalis extract in the quaternary ammonium chitosan solution, dropwise add 0.05-0.2% triphenyl phosphate solution, and centrifuge and dry to obtain quaternary ammonium chitosan nanoparticles@Sanguisorba officinalis extract.

[0016] As a preferred technical solution of the present invention, the mass ratio of the Sanguisorba officinalis extract, quaternary ammonium chitosan solution, and triphenyl phosphate solution is 1:100-200:500-1000.

[0017] As a preferred technical solution of the present invention, the preparation method of the Lithospermum erythrorhizon extract-lipoic acid complex is as follows:

[0018] B1. Dissolve lipoic acid in dimethyl sulfoxide, add N,N'-dicyclohexylcarbodiimide and 4-dimethylaminopyridine to activate the carboxyl group to generate an active ester intermediate;

[0019] B2. Dissolve Lithospermum erythrorhizon powder in dimethyl sulfoxide, dropwise add it to the activated lipoic acid solution prepared in B2, mix under nitrogen protection, and obtain shikonin-lipoic acid conjugate through purification and freeze-drying.

[0020] As a preferred technical solution of the present invention, the mass ratio of lipoic acid, dimethyl sulfoxide, N,N'-dicyclohexylcarbodiimide, and 4-dimethylaminopyridine in step B1 is 1:80-120:0.4-0.8:0.1-0.3.

[0021] As a preferred technical solution of the present invention, the mass ratio of Lithospermum erythrorhizon powder, dimethyl sulfoxide, and lipoic acid solution in step B2 is 1:50-70:90-110.

[0022] Furthermore, the preparation method of a kind of escharotic and anti-infective ointment includes the following steps:

[0023] (1) Mix and pulverize Coptis chinensis, Phellodendron amurense, and Scutellaria baicalensis, add 65-75% ethanol solution for microwave-assisted extraction, and concentrate the filtrate under reduced pressure to a relative density of 1.1-1.2 to obtain a tri-herb alcohol extract;

[0024] (2) The polygonum cuspidatum powder is decocted with 8 - 12 times its mass of deionized water for 2 - 4 h, and the filtrate is concentrated and dried to obtain the polygonum cuspidatum extract;

[0025] (3) Beeswax and sesame oil are mixed, heated in a water bath to melt at 70 - 80 °C, and then the tri - yellow alcohol extract and the polygonum cuspidatum extract are added;

[0026] (4) Cool to 30 - 40 °C, and successively add the pH - responsive nanoparticles of sanguisorba officinalis extract, the complex of lithospermum erythrorhizon extract - lipoic acid, and borneol, and mix evenly to obtain the product.

[0027] Advantages of the present invention:

[0028] (1) Through the pH / ROS dual - responsive delivery system of the present invention, targeted enrichment and active release of drugs in necrotic tissues are achieved. pH - sensitive mechanism: The pH - responsive nanoparticles of sanguisorba officinalis extract (cross - linked by quaternary ammonium chitosan - TPP) rapidly disintegrate in the acidic environment of the necrotic area, releasing the active substances in the sanguisorba officinalis extract, degrading necrotic collagen, and avoiding the exposure of healthy tissues; ROS - responsive mechanism: The shikonin - lipoic acid conjugate breaks in the necrotic area with high ROS to release shikonin, inhibiting the inflammatory pathway, and the remaining lipoic acid can scavenge ROS in healthy tissues, blocking further oxidative damage reactions.

[0029] (2) The ointment of the present invention also has antibacterial and anti - inflammatory effects through the compatibility of coptis chinensis, phellodendron amurense, scutellaria baicalensis, and polygonum cuspidatum, enhances the transdermal permeability and reduces wound pain through borneol, and the compound of beeswax and sesame oil improves the stability of the ointment property, softens when contacting the wound and releases more active substances. Detailed implementation mode

[0030] To further elaborate on the technical means and effects adopted by the present invention to achieve the predetermined invention purpose, the following examples are used to describe in detail the specific implementation mode, structure, characteristics, and their effects according to the present invention.

[0031] Example 1

[0032] An eschar - removing and granulation - promoting anti - infective ointment, the raw materials include:

[0033] Antibacterial and anti - inflammatory base material: 10 parts by weight of coptis chinensis, 10 parts by weight of phellodendron amurense, 10 parts by weight of scutellaria baicalensis, 8 parts by weight of polygonum cuspidatum;

[0034] Transdermal penetration enhancer: 2 parts by weight of borneol;

[0035] Thermosensitive matrix: 25 parts by weight of beeswax, 30 parts by weight of sesame oil;

[0036] Targeted release extractant: 6.5 parts by weight of pH - responsive nanoparticles of sanguisorba officinalis extract, 5 parts by weight of the complex of lithospermum erythrorhizon extract - lipoic acid.

[0037] The preparation method of the sanguisorba extract pH-responsive nanoparticles is as follows:

[0038] A1. Dissolve the sanguisorba powder in 70% ethanol solution, perform gradient elution through an AB-8 macroporous resin column chromatography, collect the enriched segment of sanguisorba saponins and dry it to obtain the sanguisorba extract;

[0039] A2. Dissolve the quaternary ammonium salt of chitosan in 1% acetic acid solution to prepare a solution with a concentration of 2 mg / mL of the quaternary ammonium salt of chitosan;

[0040] A3. Dissolve the sanguisorba extract in the quaternary ammonium salt of chitosan solution, dropwise add 0.1% triphenyl phosphate solution, and centrifuge and dry to obtain quaternary ammonium salt of chitosan nanoparticles @ sanguisorba extract.

[0041] The mass ratio of the sanguisorba extract, the quaternary ammonium salt of chitosan solution, and the triphenyl phosphate solution is 1:150:750.

[0042] The preparation method of the lithospermum extract-lipoic acid complex is as follows:

[0043] B1. Dissolve lipoic acid in dimethyl sulfoxide, add N,N'-dicyclohexylcarbodiimide and 4-dimethylaminopyridine to activate the carboxyl group to generate an active ester intermediate;

[0044] B2. Dissolve the lithospermum powder in dimethyl sulfoxide, dropwise add it into the activated lipoic acid solution prepared in B2, mix under nitrogen protection, and obtain the shikonin-lipoic acid conjugate through purification and freeze-drying.

[0045] The mass ratio of lipoic acid, dimethyl sulfoxide, N,N'-dicyclohexylcarbodiimide, and 4-dimethylaminopyridine in step B1 is 1:100:0.6:0.1.

[0046] The mass ratio of the lithospermum powder, dimethyl sulfoxide, and lipoic acid solution in step B2 is 1:60:100.

[0047] The preparation method of a kind of escharotic and anti-infective ointment includes the following steps:

[0048] (1) Mix and pulverize coptis, phellodendron, and scutellaria, add 70% ethanol solution for microwave-assisted extraction (400 W / 60 °C / 15 min), and concentrate the filtrate under reduced pressure to a relative density of 1.1 to obtain the alcohol extract of the three yellows;

[0049] (2) Decoct the polygonum cuspidatum powder with 10 times the mass of deionized water for 3 h, and concentrate and dry the filtrate to obtain the polygonum cuspidatum extract;

[0050] (3) Mix beeswax and sesame oil, heat them in a water bath to melt at 75 °C, and then add the alcohol extract of the three yellows and the polygonum cuspidatum extract;

[0051] (4) Cool down to 35°C and add the pH-responsive nanoparticles of sanguisorba officinalis extract, the lithospermum erythrorhizon extract-lipoic acid complex, and borneol in sequence, and mix evenly to obtain the product.

[0052] Example 2

[0053] Compared with the general anti-corrosion, muscle generation and anti-infection ointment in Comparative Example 1, this example is designed for wounds with milder conditions or children. The main difference is that the content of traditional Chinese medicine components is lower and the targeted release is slower:

[0054] An anti-corrosion, muscle generation and anti-infection ointment, the raw materials of which include:

[0055] Antibacterial and anti-inflammatory base material: 8 parts by weight of coptis chinensis, 8 parts by weight of phellodendron amurense, 8 parts by weight of scutellaria baicalensis, 6 parts by weight of polygonum cuspidatum;

[0056] Transdermal penetration enhancer: 2 parts by weight of borneol;

[0057] Thermosensitive matrix: 30 parts by weight of beeswax, 35 parts by weight of sesame oil;

[0058] Targeted release extractant: 5 parts by weight of pH-responsive nanoparticles of sanguisorba officinalis extract, 4 parts by weight of lithospermum erythrorhizon extract-lipoic acid complex.

[0059] The preparation method of the pH-responsive nanoparticles of sanguisorba officinalis extract is as follows:

[0060] A1. Dissolve the sanguisorba officinalis powder in a 60% ethanol solution, perform gradient elution through an AB-8 macroporous resin column, collect the enriched segment of sanguisorba saponins and dry it to obtain the sanguisorba officinalis extract;

[0061] A2. Dissolve chitosan quaternary ammonium salt in a 1.2% acetic acid solution to prepare a chitosan quaternary ammonium salt solution with a concentration of 3 mg / mL;

[0062] A3. Dissolve the sanguisorba officinalis extract in the chitosan quaternary ammonium salt solution, dropwise add a 0.15% triphenyl phosphate solution, and centrifuge and dry to obtain chitosan quaternary ammonium salt nanoparticles@ sanguisorba officinalis extract.

[0063] The mass ratio of the sanguisorba officinalis extract, the chitosan quaternary ammonium salt solution, and the triphenyl phosphate solution is 1:200:1000.

[0064] The preparation method of the lithospermum erythrorhizon extract-lipoic acid complex is as follows:

[0065] B1. Dissolve lipoic acid in dimethyl sulfoxide, add N,N'-dicyclohexylcarbodiimide and 4-dimethylaminopyridine to activate the carboxyl group to generate an active ester intermediate;

[0066] B2. Dissolve lithospermum erythrorhizon powder in dimethyl sulfoxide, drop it into the activated lipoic acid solution prepared in B2, mix under nitrogen protection, and obtain shikonin-lipoic acid conjugate through purification and freeze-drying.

[0067] The mass ratio of lipoic acid, dimethyl sulfoxide, N,N'-dicyclohexylcarbodiimide, and 4-dimethylaminopyridine described in step B1 is 1:100:0.4:0.1.

[0068] As a preferred technical solution of the present invention, the mass ratio of lithospermum powder, dimethyl sulfoxide, and lipoic acid solution described in step B2 is 1:70:110.

[0069] The preparation method of the anti-corrosion and muscle-generation promoting and anti-infection ointment includes the following steps:

[0070] (1) Mix and pulverize Coptis chinensis, Phellodendron amurense, and Scutellaria baicalensis, add 65% ethanol solution for microwave-assisted extraction (400W / 60°C / 15min), and concentrate the filtrate under reduced pressure to a relative density of 1.1 to obtain the alcohol extract of the three yellows.

[0071] (2) Decoct polygonum cuspidatum powder with 12 times the mass of deionized water for 4h, and concentrate and dry the filtrate to obtain polygonum cuspidatum extract.

[0072] (3) Mix beeswax and sesame oil, heat them in a water bath to melt at 70°C, and then add the alcohol extract of the three yellows and polygonum cuspidatum extract.

[0073] (4) Cool down to 30°C, and sequentially add pH-responsive nanoparticles of sanguisorba officinalis extract, lithospermum extract-lipoic acid complex, and borneol, and mix evenly to obtain the product.

[0074] Example 3

[0075] Comparing with the general anti-corrosion and muscle-generation promoting and anti-infection ointment in Comparative Example 1, this example is designed for more severe wounds. The main difference is that the content of traditional Chinese medicine and borneol components is higher, and the targeted release is faster:

[0076] An anti-corrosion and muscle-generation promoting and anti-infection ointment, the raw materials include:

[0077] Antibacterial and anti-inflammatory base material: 12 parts by weight of Coptis chinensis, 12 parts by weight of Phellodendron amurense, 12 parts by weight of Scutellaria baicalensis, 6-10 parts by weight of polygonum cuspidatum;

[0078] Transdermal penetration enhancer: 3 parts by weight of borneol;

[0079] Thermosensitive matrix: 20 parts by weight of beeswax, 25 parts by weight of sesame oil;

[0080] Targeted release extractant: 8 parts by weight of pH-responsive nanoparticles of sanguisorba officinalis extract, 6 parts by weight of lithospermum extract-lipoic acid complex.

[0081] The preparation method of the pH-responsive nanoparticles of sanguisorba officinalis extract is as follows:

[0082] A1. Dissolve sanguisorba officinalis powder in 80% ethanol solution, perform gradient elution through an AB-8 macroporous resin column, collect the enriched section of sanguisorba saponins and dry it to obtain sanguisorba officinalis extract;

[0083] A2. Dissolve chitosan quaternary ammonium salt in 1.2% acetic acid solution to prepare a solution with a chitosan quaternary ammonium salt concentration of 3 mg / mL;

[0084] A3. Dissolve the sanguisorba extract in the chitosan quaternary ammonium salt solution, add 0.2% triphenyl phosphate solution, and centrifuge and dry to obtain chitosan quaternary ammonium salt nanoparticles @ sanguisorba extract.

[0085] The mass ratio of the sanguisorba extract, chitosan quaternary ammonium salt solution, and triphenyl phosphate solution is 1:100:500.

[0086] The preparation method of the lithospermum extract-lipoic acid complex is as follows:

[0087] B1. Dissolve lipoic acid in dimethyl sulfoxide, add N,N'-dicyclohexylcarbodiimide and 4-dimethylaminopyridine to activate the carboxyl group to generate an active ester intermediate;

[0088] B2. Dissolve lithospermum powder in dimethyl sulfoxide, drop it into the activated lipoic acid solution prepared in B2, mix under nitrogen protection, and obtain lithospermin-lipoic acid conjugate through purification and freeze-drying.

[0089] The mass ratio of lipoic acid, dimethyl sulfoxide, N,N'-dicyclohexylcarbodiimide, and 4-dimethylaminopyridine in step B1 is 1:120:0.8:0.3.

[0090] The mass ratio of lithospermum powder, dimethyl sulfoxide, and lipoic acid solution in step B2 is 1:50:110.

[0091] The preparation method of the anti-corrosion and muscle-growing and anti-infection ointment includes the following steps:

[0092] (1) Mix and pulverize coptis, phellodendron, and scutellaria, add 75% ethanol solution for microwave-assisted extraction (400 W / 60 °C / 15 min), and concentrate the filtrate under reduced pressure to a relative density of 1.2 to obtain a tri-yellow alcohol extract;

[0093] (2) Decoct polygonum cuspidatum powder with 8 times its mass of deionized water for 4 h, and concentrate and dry the filtrate to obtain a polygonum cuspidatum extract;

[0094] (3) Mix beeswax and sesame oil, heat them in a water bath to melt at 80 °C, and then add the tri-yellow alcohol extract and polygonum cuspidatum extract;

[0095] (4) Cool down to 40 °C, and sequentially add pH-responsive nanoparticles of sanguisorba extract, lithospermum extract-lipoic acid complex, and borneol, and mix evenly to obtain the product.

[0096] Comparative Example 1

[0097] On the basis of Example 1, the pH-responsive nanoparticles of sanguisorba extract in the raw materials were changed to directly add sanguisorba extract, 2 mg / mL chitosan quaternary ammonium salt solution (containing 1% acetic acid) and 0.1% triphenyl phosphate solution with a mass ratio of 1:150:750; the rest was the same as in Example 1.

[0098] Comparative Example 2

[0099] On the basis of Example 1, the lithospermum extract-lipoic acid complex in the raw materials was changed to directly add lithospermum powder and lipoic acid solution with a mass ratio of 1:100, and the rest was the same as in Example 1.

[0100] Comparative Example 3

[0101] On the basis of Example 1, the pH-responsive nanoparticles of sanguisorba extract in the raw materials were changed to directly add sanguisorba extract, 2 mg / mL chitosan quaternary ammonium salt solution (containing 1% acetic acid) and 0.1% triphenyl phosphate solution with a mass ratio of 1:150:750; the lithospermum extract-lipoic acid complex was changed to directly add lithospermum powder and lipoic acid solution with a mass ratio of 1:100; the rest was the same as in Example 1.

[0102] Example 4

[0103] On the basis of Example 1, the antibacterial and anti-inflammatory base material was changed to: 20 parts by weight of phellodendron bark, 10 parts by weight of scutellaria baicalensis, 8 parts by weight of polygonum cuspidatum, and the rest was the same as in Example 1.

[0104] Example 5

[0105] On the basis of Example 1, the antibacterial and anti-inflammatory base material was changed to: 10 parts by weight of coptis chinensis, 20 parts by weight of scutellaria baicalensis, 8 parts by weight of polygonum cuspidatum, and the rest was the same as in Example 1.

[0106] Example 6

[0107] On the basis of Example 1, the antibacterial and anti-inflammatory base material was changed to: 10 parts by weight of coptis chinensis, 10 parts by weight of phellodendron bark, 18 parts by weight of polygonum cuspidatum, and the rest was the same as in Example 1.

[0108] Example 7

[0109] On the basis of Example 1, the antibacterial and anti-inflammatory base material was changed to: 18 parts by weight of coptis chinensis, 10 parts by weight of phellodendron bark, 10 parts by weight of scutellaria baicalensis, and the rest was the same as in Example 1.

[0110] Example 8

[0111] On the basis of Example 1, the temperature-sensitive matrix was changed to: 25 parts by weight of beeswax, 30 parts by weight of glycerol, and the rest was the same as in Example 1.

[0112] Example 9

[0113] On the basis of Example 1, the temperature-sensitive matrix is changed to: 25 parts by weight of soybean wax and 30 parts by weight of sesame oil, and the rest is the same as in Example 1.

[0114] Performance test:

[0115] Antibacterial test: According to the antibacterial circle diameter of Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans was tested according to the antibacterial efficacy test method in General Principles 1121 of the Third Edition of the Chinese Pharmacopoeia 2020;

[0116] Anti-inflammatory, necrotic tissue clearance and healthy skin protection test: 36 trauma volunteers were recruited and randomly divided into 12 groups. The ointments prepared in Examples 1-3 and Comparative Examples 1-9 were applied twice a day, morning and evening, at 0.2 g / cm 2 , and the necrotic tissue clearance time (the number of days required for the necrotic area to be reduced to 10% of the initial value from the start of treatment) was observed;

[0117] The inflammation condition was observed 3 days after drug administration: Scored according to "Best Practice Principles for Infected Wounds in Clinical Practice", "0 points: no redness / warmth / pain / exudation; 1 point: mild (erythema <2 mm, slightly warm to palpation, occasional pain, small amount of exudation); 2 points: moderate (erythema 2-5 mm, obvious warmth, persistent pain, moderate amount of exudation)"; 3 points: severe (erythema >5 mm, burning sensation, severe pain, large amount of exudation)

[0118] Gentle test: The skin redness range (mm) around the wound was measured 3 days after drug administration.

[0119]

[0120] It can be seen from the test results that due to the high-activity targeting components in the examples, the necrotic tissue clearance time is the shortest. In Comparative Examples 1-3, there is no targeting carrier, and the drug is randomly released, and the clearance time is extended to 13-18 days, and the inflammatory reaction (redness range) is significantly aggravated; in Example 2 (low dose), the redness range is the smallest (1.8 mm), which is suitable for children or mild wounds; although Example 3 has the fastest clearance, the redness range is slightly higher (2.5 mm); the lack of targeting carrier (Comparative Examples 1-3) leads to the efficacy returning to the traditional level, verifying the necessity of nanoparticles and conjugates; the imbalance of matrix or base material ratio (Comparative Examples 4-9) significantly affects the efficacy.

[0121] The above are only the preferred embodiments of the present invention and do not impose any form of limitation on the present invention. Although the present invention has been disclosed above in the preferred embodiments, it is not intended to limit the present invention. Any person skilled in the art can make some changes or modifications to the above-disclosed technical content to obtain equivalent embodiments with equivalent changes within the scope of the technical solution of the present invention. However, as long as it does not depart from the content of the technical solution of the present invention, any indirect modification, equivalent change and modification made to the above embodiments based on the technical essence of the present invention still fall within the scope of the technical solution of the present invention.

Claims

1. A medicinal ointment for removing putrefaction and promoting granulation and anti-infection, characterized in that: The raw materials include: 8 - 12 parts by weight of Coptis chinensis, 8 - 12 parts by weight of Phellodendron amurense, 8 - 12 parts by weight of Scutellaria baicalensis, 6 - 10 parts by weight of Polygonum cuspidatum, 1 - 3 parts by weight of borneol, 20 - 30 parts by weight of beeswax, 25 - 35 parts by weight of sesame oil, and 9 - 14 parts by weight of a necrotic tissue - targeted release extractant; The necrotic tissue - targeted release extractant: 5 - 8 parts by weight of pH - responsive nanoparticles of Sanguisorba officinalis extract, 4 - 6 parts by weight of a complex of Lithospermum erythrorhizon extract and lipoic acid.

2. The anti - putrefactive, muscle - generating and anti - infective ointment according to claim 1, wherein: The preparation method of the pH - responsive nanoparticles of Sanguisorba officinalis extract is as follows: A1. Dissolve Sanguisorba officinalis powder in an ethanol solution, perform gradient elution through a macroporous resin column chromatography, collect the enriched section of saponins of Sanguisorba officinalis and dry it to obtain Sanguisorba officinalis extract; A2. Dissolve quaternary ammonium salt of chitosan in an acetic acid solution; A3. Dissolve Sanguisorba officinalis extract in the quaternary ammonium salt of chitosan solution, dropwise add triphenyl phosphate solution, and perform centrifugation and drying to obtain quaternary ammonium salt of chitosan nanoparticles@Sanguisorba officinalis extract.

3. The anti - putrefactive, muscle - promoting and anti - infective ointment according to claim 2, characterized in that: The concentration of the acetic acid solution in step A2 is 0.8 - 1.2%; the concentration of the quaternary ammonium salt of chitosan is 1 - 3 mg / mL.

4. The anti - putrefactive, muscle - promoting and anti - infective ointment according to claim 2, characterized in that: The concentration of the triphenyl phosphate solution in step A3 is 0.05 - 0.2%; the mass ratio of the Sanguisorba officinalis extract, the quaternary ammonium salt of chitosan solution, and the triphenyl phosphate solution is 1:100 - 200:500 - 1000.

5. The debriding and myogenic anti-infection ointment according to claim 1, characterized in that: The preparation method of the complex of Lithospermum erythrorhizon extract and lipoic acid is as follows: B1. Dissolve lipoic acid in dimethyl sulfoxide, add N,N'-dicyclohexylcarbodiimide and 4 - dimethylaminopyridine to activate the carboxyl group to generate an active ester intermediate; B2. Dissolve Lithospermum erythrorhizon powder in dimethyl sulfoxide, dropwise add it into the activated lipoic acid solution prepared in B2, mix under nitrogen protection, and obtain shikonin - lipoic acid conjugate through purification and lyophilization.

6. The anti - putrefactive, myogenic and anti - infective ointment according to claim 5, characterized in that: The mass ratio of lipoic acid, dimethyl sulfoxide, N,N'-dicyclohexylcarbodiimide, and 4 - dimethylaminopyridine in step B1 is 1:80 - 120:0.4 - 0.8:0.1 - 0.

3.

7. The anti - putrefactive and myogenic and anti - infective ointment according to claim 5, characterized in that: The mass ratio of Lithospermum erythrorhizon powder, dimethyl sulfoxide, and lipoic acid solution in step B2 is 1:50 - 70:90 - 110.

8. A method for preparing the anti - putrefactive and myogenic anti - infective ointment according to any one of claims 1 - 7, characterized in that: It includes the following steps: (1) Mix and pulverize Coptis chinensis, Phellodendron amurense, and Scutellaria baicalensis, add an ethanol solution for extraction, and concentrate the filtrate under reduced pressure to obtain a tri - yellow alcohol extract; (2) Decoct Polygonum cuspidatum powder with deionized water, and concentrate and dry the filtrate to obtain Polygonum cuspidatum extract; (3) Mix beeswax and sesame oil, heat and melt them in a water bath, and then add the tri - yellow alcohol extract and Polygonum cuspidatum extract; (4) Sequentially add the pH - responsive nanoparticles of Sanguisorba officinalis extract, the complex of Lithospermum erythrorhizon extract and lipoic acid, and borneol, and mix evenly to obtain the product.

9. The preparation method of a necrotic tissue-removing, muscle-growing and anti-infection ointment according to claim 8, characterized in that: The concentration of the ethanol solution in step (1) is 65 - 75%; the filtrate is concentrated under reduced pressure to a relative density of 1.1 - 1.

2.

10. The preparation method of a necrotic tissue-removing, muscle-growing and anti-infective ointment according to claim 8, characterized in that: The mass ratio of Polygonum cuspidatum powder to deionized water in step (2) is 1:8 - 12; the decocting time is 2 - 4 h.

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