Sample introduction device and microfluidic system

Abstract

The invention discloses a sampling device and a microfluidic system, and belongs to the technical field of microfluidics. According to the sample introduction device and the microfluidic system, the reagent can be introduced into the distribution cavity from the sample introduction port, then the reagent is directly distributed to the plurality of distribution runners at the same time by the distribution cavity, and the size of the distribution cavity and the size of the distribution runners are limited, so that the distribution cavity can uniformly distribute the reagent to the plurality of distribution runners; compared with the prior art, the sample injection device has the advantages that the structural complexity of the sample injection device is reduced, the number of liquid separation stages of the sample injection device is increased, the productivity of the microfluidic system is improved, and the particle size of a product prepared by the microfluidic system is more uniform.

Description

Technical Field

[0001] The present invention relates to the technical field of microfluidics, and in particular to a sample introduction device and a microfluidic system. Background Art

[0002] Microfluidic technology is a technology for precisely manipulating fluids at the micron scale, which can flexibly combine or scale-integrate various functional units such as sample preparation, reaction, separation, and detection in chemical, biological, and other experimental processes on a tiny platform. The micron-scale pipeline structure on a microfluidic chip greatly increases the area / volume ratio of the fluid, realizes an efficient mass transfer ratio, improves the reaction efficiency, and makes the experiment more controllable; at the same time, the integration of each functional unit by microfluidic technology can avoid misoperations caused by complex processes. Benefiting from this, the application of microfluidic technology in the fields of microreactors and micron / nano material preparation has been deeply explored and studied.

[0003] Since the channel size on a microfluidic chip is only at the micron scale, the output that a single microfluidic chip can achieve is limited and cannot meet the industrial mass production requirements. Generally, multiple parallel units are designed on a single microfluidic chip to increase the production capacity per unit time. How to ensure the uniform flow rate between the parallel units is a key difficulty. Currently, the step-by-step dichotomy method is generally adopted, that is, dividing into two, two into four, four into eight, and so on, and then realizing the parallel operation of multiple units. This structure has low distribution efficiency. The single-stage liquid separation can only be divided into two units. If you want to increase the number of parallel units, you can only increase the number of liquid separation stages, which means that the complexity of the liquid separation device structure increases step by step. Summary of the Invention

[0004] The purpose of the present invention is to provide a sample introduction device and a microfluidic system, which not only reduce the structural complexity of the sample introduction device, but also make the particle size of the obtained product more uniform.

[0005] To achieve the above purpose, the following technical solutions are provided:

[0006] A sample introduction device, comprising:

[0007] A sample introduction member, the sample introduction member includes a distribution cavity and a sample introduction port communicated with the distribution cavity. An outer side of the sample introduction member is provided with a sample introduction surface, and the sample introduction surface is provided with a plurality of distribution channels all communicated with the distribution cavity. The plurality of distribution channels are arranged at intervals along a first direction;

[0008] Along the first direction, a length of the distribution cavity between two adjacent distribution channels is l d , a width of the distribution channel is w b ;

[0009] Along a second direction, a width of the distribution cavity is w d , a depth of the distribution channel is h b ;

[0010] Along the third direction, the depth of the distribution cavity is h d , and the length of the distribution channel is l b ;

[0011] Along the first direction, h d remains unchanged, and w d remains unchanged;

[0012] Along the third direction, h b remains unchanged, and w b remains unchanged;

[0013] ;

[0014] The first direction, the second direction and the third direction are arranged at an angle to each other in pairs.

[0015] As a preferred technical solution of the sampling device, the sampling member includes a housing and a cover. The sampling surface is provided on the housing. A groove is provided on the housing or the cover. The cover is connected to the housing to close the notch of the groove and form the distribution cavity.

[0016] As a preferred technical solution of the sampling device, the housing is further provided with connection channels corresponding to the plurality of distribution channels one by one. The distribution channels communicate with the distribution cavity through the corresponding connection channels.

[0017] As a preferred technical solution of the sampling device, the distribution cavity and the distribution channels are respectively located on both sides of the housing.

[0018] As a preferred technical solution of the sampling device, the sampling surface is further provided with a collection channel for communicating with the collection device. The distribution cavity and the distribution channels are isolated from each other with respect to the collection channel.

[0019] As a preferred technical solution of the sampling device, the sampling device further includes distribution seals corresponding to the plurality of distribution channels one by one. The distribution seals are sleeved on the outer sides of the corresponding distribution channels;

[0020] The sampling device further includes collection seals corresponding to the plurality of collection channels one by one. The collection seals are sleeved on the outer sides of the corresponding collection channels.

[0021] As a preferred technical solution of the sampling device, the plurality of distribution seals are integrally formed; or, the plurality of distribution seals are separately provided and fixedly connected;

[0022] The plurality of collection seals are integrally formed; or, the plurality of collection seals are separately provided and fixedly connected.

[0023] As a preferred technical solution of the sample injection device, the sample injection surface is provided with distribution avoidance grooves corresponding to the plurality of distribution seals, and the distribution seals are embedded in the distribution avoidance grooves;

[0024] The sample injection surface is provided with collection avoidance grooves corresponding to the plurality of collection seals, and the collection seals are embedded in the collection avoidance grooves.

[0025] As a preferred technical solution of the sample injection device, the sample injection device further includes a temperature regulating member for regulating the temperature of the reagent in the distribution cavity and / or the distribution flow channel; and / or,

[0026] The sample injection device further includes a heat preservation member sleeved outside the sample injection member, and the sample injection surface is located outside the heat preservation member.

[0027] As a preferred technical solution of the sample injection device, the first direction, the second direction and the third direction are perpendicular to each other in pairs.

[0028] To achieve the above object, a microfluidic system is further provided, including at least one juxtaposed microfluidic unit. The microfluidic unit includes a microfluidic chip and at least one sample injection device as described in any one of the above. The microfluidic chip is disposed on the sample injection surface. The microfluidic chip includes a plurality of channel units corresponding to the plurality of distribution flow channels of the sample injection device. The channel unit includes a plurality of microfluidic channels spaced apart along the third direction and isolated from each other, and the distribution flow channel communicates with the plurality of microfluidic channels of the corresponding channel unit.

[0029] As a preferred technical solution of the microfluidic system, along the third direction, the length of the distribution flow channel between two adjacent microfluidic channels is L d ;

[0030] Along the axial direction of the microfluidic channel, the length of the microfluidic channel is L b ;

[0031] The width of the microfluidic channel is W b ;

[0032] Along the second direction, the depth of the microfluidic channel is H b ;

[0033] Along the axial direction of the microfluidic channel, H b remains unchanged, W b remains unchanged;

[0034] ;

[0035] The second direction, the width direction of the microfluidic channel, and the axial direction of the microfluidic channel are perpendicular to each other in pairs.

[0036] As a preferred technical solution of the microfluidic system, there are two sampling devices, the microfluidic chip includes two connection surfaces, and the two connection surfaces are respectively connected to the sampling surfaces of the two sampling devices;

[0037] The microfluidic channel includes a microfluidic outlet and two microfluidic inlets, the two microfluidic inlets are respectively located on the two connection surfaces, and the microfluidic outlet is located on one of the connection surfaces;

[0038] In the same channel unit, the two microfluidic inlets of multiple microfluidic channels are respectively communicated with the corresponding distribution channels of the two sampling devices, and the microfluidic outlets of multiple microfluidic channels are all used to communicate with the collection device.

[0039] As a preferred technical solution of the microfluidic system, at least one collection channel corresponding to multiple channel units is further provided on the sampling surface of one of the sampling devices, the microfluidic outlets of the multiple channel units are all communicated with the corresponding collection channel, and the collection channel is communicated with the collection device.

[0040] As a preferred technical solution of the microfluidic system, the two sampling devices of the same microfluidic unit are respectively a first sampling device and a second sampling device;

[0041] There are multiple microfluidic units, the sampling parts of the first sampling devices of the multiple microfluidic units are connected to form a total sampling part, and the distribution cavities of the first sampling devices of the multiple microfluidic units are communicated to form a total distribution cavity.

[0042] As a preferred technical solution of the microfluidic system, the total sampling part includes multiple sampling surfaces, and the multiple sampling surfaces are sequentially arranged on the outer peripheral side of the total sampling part along a first circumferential direction.

[0043] As a preferred technical solution of the microfluidic system, the total distribution cavity includes a first distribution sub-cavity and second distribution sub-cavities corresponding to the multiple sampling surfaces of the total sampling part one by one. The sampling port and the multiple second distribution sub-cavities are all communicated with the first distribution sub-cavity, and the multiple second distribution sub-cavities are sequentially communicated along the first circumferential direction to form a total distribution sub-cavity with an annular structure.

[0044] As a preferred technical solution of the microfluidic system, the total sampling part includes:

[0045] A first housing, and multiple sampling surfaces are sequentially arranged on the outer peripheral side of the first housing along the first circumferential direction;

[0046] A second housing, which is disposed inside the first housing, and a total distribution cavity is formed between the first housing and the second housing.

[0047] As a preferred technical solution of the microfluidic system, along the third direction, one end of the second housing abuts against the inner wall of the first housing, a first distribution sub-cavity is formed between the other end of the second housing and the inner wall of the first housing, and a total distribution sub-cavity is formed between the outer peripheral side of the second housing around the third direction and the inner wall of the first housing.

[0048] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0049] For the sampling device and the microfluidic system of the present invention, the reagent can be introduced into the distribution cavity from the sampling port, and then the reagent can be directly distributed to a plurality of distribution channels simultaneously from the distribution cavity. By such that the distribution cavity can evenly distribute the reagent to a plurality of distribution channels, and the flow rates of the reagent in each distribution channel are consistent. Compared with the prior art, not only the structural complexity of the sampling device is reduced, thereby facilitating an increase in the liquid separation stage number of the sampling device to improve the production capacity of the microfluidic system, but also the particle size of the product obtained by the microfluidic system can be made more uniform. Description of the Drawings

[0050] Figure 1 It is the first structural schematic diagram of the microfluidic system in the embodiment of the present invention;

[0051] Figure 2 It is the second structural schematic diagram of the microfluidic system in the embodiment of the present invention;

[0052] Figure 3 It is the first structural schematic diagram of the first sampling device in the embodiment of the present invention;

[0053] Figure 4 It is Figure 3 the cross-sectional view of the A-A plane of;

[0054] Figure 5 It is the second structural schematic diagram of the first sampling device in the embodiment of the present invention;

[0055] Figure 6 It is the first structural schematic diagram of the second sampling device in the embodiment of the present invention;

[0056] Figure 7 It is Figure 6 the cross-sectional view of the B-B plane of;

[0057] Figure 8 It is the structural schematic diagram of the first sampling device and the microfluidic chip in the embodiment of the present invention;

[0058] Figure 9 For Figure 8 the enlarged view at C;

[0059] Figure 10 It is a schematic cross-sectional view of the microfluidic channel in the embodiment of the present invention;

[0060] Figure 11 It is a schematic structural view of the second sample injection device and the microfluidic chip in the embodiment of the present invention;

[0061] Figure 12 It is the third schematic structural view of the microfluidic system in the embodiment of the present invention;

[0062] Figure 13 It is a cross-sectional view of the total sample injection part in the embodiment of the present invention;

[0063] Figure 14 It is a schematic structural view of the total sample injection part in the embodiment of the present invention;

[0064] Figure 15 It is the second schematic structural view of the second sample injection device in the embodiment of the present invention;

[0065] Figure 16 It is a photograph of a droplet prepared by a microfluidic system in the prior art;

[0066] Figure 17 It is a photograph of a droplet prepared by the microfluidic system using the injection device of the first specification in the embodiment of the present invention;

[0067] Figure 18 It is a photograph of a droplet prepared by the microfluidic system using the injection device of the second specification in the embodiment of the present invention.

[0068] Reference numerals:

[0069] 1a, the first sample injection device; 1b, the second sample injection device; 11, the sample injection part; 111, the distribution cavity; 112, the sample injection port; 113, the sample injection surface; 1131, the distribution flow channel; 1132, the collection flow channel; 1133, the sealing avoidance groove; 114, the housing; 1141, the groove; 1142, the connection channel; 115, the cover body; 116, the collection channel; 12, the sample injection joint; 13, the collection joint; 141, the groove seal; 142, the flow channel seal; 15, the temperature adjustment part; 16, the heat preservation part; 11a, the total sample injection part; 111a, the total distribution cavity; 111a1, the first distribution sub-cavity; 111a2, the second distribution sub-cavity; 114a, the first housing; 114a1, the housing part; 114a2, the cover part; 114a3, the housing seal; 114b, the second housing; 2, the microfluidic chip; 211, the microfluidic channel; 2111, the microfluidic inlet; 2112, the microfluidic outlet; 221, the first connection surface; 222, the second connection surface. Detailed implementation manners

[0070] To make the objectives, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Apparently, the described embodiments are some but not all of the embodiments of the present invention. Components of the embodiments of the present invention described and illustrated in the drawings herein can be arranged and designed in a variety of different configurations.

[0071] Therefore, the following detailed description of the embodiments of the present invention provided in the drawings is not intended to limit the scope of the claimed invention, but merely represents selected embodiments of the present invention. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the scope of protection of the present invention.

[0072] It should be noted that like reference numerals and letters denote like items in the following drawings. Therefore, once an item is defined in one drawing, it does not require further definition and explanation in subsequent drawings.

[0073] In the description of the present invention, it should be noted that the orientation or positional relationship indicated by the terms "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. is based on the orientation or positional relationship shown in the drawings, or the orientation or positional relationship in which the inventive product is customarily placed during use. It is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as a limitation of the present invention. In addition, the terms "first", "second", "third", etc. are only used for descriptive distinction and cannot be understood as indicating or implying relative importance. In the description of the present invention, unless otherwise specified, the meaning of "a plurality" is two or more.

[0074] In the description of the present invention, it should also be noted that unless otherwise clearly defined and limited, the terms "installed" and "connected" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0075] In the present invention, unless otherwise clearly specified and defined, the first feature being "on" or "under" the second feature may include direct contact between the first and second features, or may include the first and second features not being in direct contact but in contact through additional features therebetween. Moreover, the first feature being "above", "over" and "on top of" the second feature includes the first feature being directly above and obliquely above the second feature, or merely indicating that the horizontal height of the first feature is higher than that of the second feature. The first feature being "under", "beneath" and "underneath" the second feature includes the first feature being directly below and obliquely below the second feature, or merely indicating that the horizontal height of the first feature is lower than that of the second feature.

[0076] Embodiments of the present invention will be described in detail below. Examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below by referring to the accompanying drawings are exemplary and are only used to explain the present invention and should not be construed as a limitation of the present invention.

[0077] Embodiment 1

[0078] As Figures 1 - 11 shown, this embodiment provides a sample introduction device and a microfluidic system. The microfluidic system includes a sample introduction device and a microfluidic chip 2. The sample introduction device is used to introduce a reagent into the microfluidic channel 211 of the microfluidic chip 2.

[0079] As Figures 1 - 5 shown, the sample introduction device includes a sample introduction member 11. The sample introduction member 11 includes a distribution cavity 111 and a sample introduction port 112 communicating with the distribution cavity 111. An outer side of the sample introduction member 11 is provided with a sample introduction surface 113. The sample introduction surface 113 is provided with a plurality of distribution channels 1131 all communicating with the distribution cavity 111. The plurality of distribution channels 1131 are arranged at intervals along a first direction.

[0080] Along the first direction, the length of the distribution cavity 111 between two adjacent distribution channels 1131 is l d , the width of the distribution channel 1131 is w b ; along a second direction, the width of the distribution cavity 111 is w d , the depth of the distribution channel 1131 is h b ; along a third direction, the depth of the distribution cavity 111 is h d , the length of the distribution channel 1131 is l b ; along the first direction, h d remains unchanged, w d remains unchanged; along the third direction, h b remains unchanged, w b remains unchanged; .

[0081] The first direction, the second direction, and the third direction are arranged at an angle to each other in pairs.

[0082] It should be noted that along the first direction, the length of the distribution cavity 111 between two adjacent distribution channels 1131 is the length of the distribution cavity 111 between the axes of two adjacent distribution channels 1131. It can be understood that the axis of the distribution channel 1131 is parallel to the third direction.

[0083] In the sample injection device of this embodiment, only one sample injection port 112 is provided, which is beneficial to ensuring the consistency of the reagent flow rates in each distribution channel 1131.

[0084] In the sample injection device of this embodiment, a single-stage liquid separation method is adopted. That is to say, after the reagent is introduced into the distribution cavity 111 from the sample injection port 112, the distribution cavity 111 directly distributes the reagent to a plurality of distribution channels 1131 simultaneously. Compared with the prior art, it not only greatly reduces the structural complexity of the sample injection device, but also efficiently realizes the amplification of the fluid flux of the microfluidic chip 2 and improves the production capacity of the microfluidic system. By making , the distribution cavity 111 can evenly distribute the reagent to a plurality of distribution channels 1131, and the flow rates of the reagent in each distribution channel 1131 are consistent, and the particle size of the product obtained by the microfluidic system can be made more uniform.

[0085] In this embodiment, the first direction, the second direction, and the third direction are perpendicular to each other in pairs.

[0086] Specifically, the cross-section of the distribution cavity 111 is rectangular; the cross-section of the distribution cavity 111 is perpendicular to the first direction. The cross-section of the distribution channel 1131 is rectangular; the cross-section of the distribution channel 1131 is perpendicular to the third direction.

[0087] As an alternative, among the first direction, the second direction, and the third direction, the angle between any two directions can also be approximately 90°, or the angle between any two directions can be near 90°. It should be noted that the value range of the angle between any two directions in this embodiment is not limited.

[0088] Optionally, as Figure 4 and Figure 7As shown in the figure, the sample introduction part 11 includes a housing 114 and a cover 115. The sample introduction surface 113 is provided on the housing 114. The housing 114 is further provided with a groove 1141. The cover 115 is connected to the housing 114 to close the notch of the groove 1141 and form a distribution cavity 111. That is to say, the cover 115 is covered on the notch of the groove 1141. By connecting the cover 115 and the housing 114, the notch of the groove 1141 can be closed to form the distribution cavity 111, which is convenient for processing the distribution cavity 111 and assembling the cover 115 and the housing 114. It should be noted that the sample introduction surface 113 is provided on the outer surface of the housing 114, and the connection between the housing 114 and the cover 115 cannot block the sample introduction surface 113, so that the microfluidic chip 2 can be arranged on the sample introduction surface 113. Of course, the groove 1141 can also be provided on the cover 115. By connecting the cover 115 and the housing 114, the housing 114 can close the notch of the groove 1141 to form the distribution cavity 111.

[0089] Exemplarily, the housing 114 and the cover 115 are connected by fasteners such as screws, which is convenient for disassembly and assembly.

[0090] Optionally, the sample introduction port 112 is used to connect with a reagent supply device. The sample introduction port 112 and the distribution channel 1131 are respectively arranged on both sides of the housing 114. That is to say, the sample introduction port 112 can be arranged on any side of the sample introduction part 11 where the distribution channel 1131 is not provided, which can avoid interference between the microfluidic chip 2 and the sample introduction port 112 and improve the operation convenience of the sample introduction device.

[0091] Optionally, the sample introduction port 112 is provided with a sample introduction joint 12. The sample introduction port 112 is connected to the reagent supply device through the sample introduction joint 12, which has higher operation convenience. Exemplarily, the sample introduction joint 12 is connected to the sample introduction port 112 by threads, which is convenient for disassembly and assembly and has good sealing performance.

[0092] Optionally, the sample introduction device further includes a groove seal 141 corresponding to the groove 1141. The groove seal 141 is sleeved on the outside of the groove 1141, and the groove seal 141 is clamped between the housing 114 and the cover 115, which can improve the sealing performance of the distribution cavity 111. Exemplarily, the groove seal 141 is an O-ring.

[0093] Optionally, as Figure 3 shown in the figure, the housing 114 is further provided with connection channels 1142 corresponding to the plurality of distribution channels 1131 one by one. The distribution channels 1131 are communicated with the distribution cavity 111 through the corresponding connection channels 1142. Since the distribution channels 1131 are provided on the outer surface of the housing 114, connecting the distribution channels 1131 and the distribution cavity 111 through the connection channels 1142 is convenient for ensuring the sealing performance of the distribution cavity 111 and facilitating the processing of the distribution cavity 111 and the distribution channels 1131.

[0094] Optionally, as Figure 4 shown, the dispensing chamber 111 and the dispensing flow channel 1131 are respectively located on both sides of the housing 114, that is to say, the groove 1141 and the dispensing flow channel 1131 are respectively located on both sides of the housing 114, thereby avoiding interference between the cover 115 and the microfluidic chip 2 and facilitating assembly.

[0095] Optionally, as Figure 6 and Figure 7 shown, the sampling surface 113 is further provided with a collection flow channel 1132 for communicating with the collection device, and the dispensing chamber 111 and the dispensing flow channel 1131 are both isolated from the collection flow channel 1132.

[0096] It should be noted that, as Figure 8 and Figure 11 shown, the microfluidic channel 211 of the microfluidic chip 2 includes a microfluidic inlet 2111 and a microfluidic outlet 2112. The dispensing flow channel 1131 is used to communicate with the microfluidic inlet 2111 to introduce a reagent into the microfluidic channel 211 through the microfluidic inlet 2111. The collection flow channel 1132 is used to communicate with the microfluidic outlet 2112 so that the product formed in the microfluidic channel 211 can be discharged from the microfluidic outlet 2112 into the collection flow channel 1132 and finally collected centrally by the collection device. The above setting of the collection flow channel 1132 can improve the convenience of product collection, and the manufacturing process of the collection flow channel 1132 is simple, which is beneficial to reducing costs.

[0097] It can be understood that the microfluidic chip 2 is usually provided with a plurality of microfluidic channels 211. Further, a plurality of collection flow channels 1132 are provided, so that the microfluidic outlets 2112 of the plurality of microfluidic channels 211 of the microfluidic chip 2 are respectively communicated with the plurality of collection flow channels 1132.

[0098] Optionally, as Figure 6 shown, the sampling device further includes collection connectors 13 provided in one-to-one correspondence with the collection flow channels 1132. The sampling member 11 is further provided with collection channels 116 provided in one-to-one correspondence with the collection flow channels 1132. The collection connectors 13 are installed at one end of the corresponding collection channels 116, and the other end of the collection channels 116 is communicated with the corresponding collection flow channels 1132, and then connected to the collection device through the collection connectors 13 to improve convenience.

[0099] Optionally, the sampling device further includes dispensing seals provided in one-to-one correspondence with the plurality of dispensing flow channels 1131. The dispensing seals are sleeved on the outer sides of the corresponding dispensing flow channels 1131. After the sampling device is assembled with the microfluidic chip 2, the sampling member 11 and the microfluidic chip 2 can clamp the dispensing seals, which can not only improve the sealing performance of the dispensing flow channels 1131 and avoid the problem of liquid leakage, but also facilitate assembly.

[0100] Optionally, the sampling device further includes collection seals provided in one-to-one correspondence with the plurality of collection channels 1132. The collection seals are sleeved on the outer sides of the corresponding collection channels 1132. Thus, after the sampling device is assembled with the microfluidic chip 2, the sampling member 11 and the microfluidic chip 2 can clamp the collection seals, which can not only improve the sealing performance of the collection channels 1132 and avoid liquid leakage, but also facilitate assembly.

[0101] Optionally, the plurality of dispensing seals are integrally formed; or, the plurality of dispensing seals are separately provided and fixedly connected. In other words, the plurality of dispensing seals form a whole, which is beneficial to improving the assembly convenience of the dispensing seals and the assembly efficiency. Exemplarily, the plurality of dispensing seals are integrally formed by an injection molding process; or, the plurality of separately provided dispensing seals are fixedly connected by means such as bonding.

[0102] Optionally, the plurality of collection seals are integrally formed; or, the plurality of collection seals are separately provided and fixedly connected. In other words, the plurality of collection seals form a whole, which is beneficial to improving the assembly convenience of the collection seals and the assembly efficiency. Exemplarily, the plurality of collection seals are integrally formed by an injection molding process; or the plurality of separately provided collection seals are fixedly connected by means such as bonding.

[0103] In this embodiment, when only the dispensing channels 1131 are provided on the sampling surface 113, the plurality of dispensing seals located on the sampling surface 113 are integrally formed or separately provided and fixedly connected to form an integral channel seal 142. When both the dispensing channels 1131 and the collection channels 1132 are provided on the sampling surface 113, the plurality of dispensing seals and the plurality of collection seals located on the sampling surface 113 are integrally formed or separately provided and fixedly connected to form an integral channel seal 142.

[0104] Optionally, the sampling surface 113 is provided with dispensing avoidance grooves corresponding to the plurality of dispensing seals, and the dispensing seals are embedded in the dispensing avoidance grooves, so as to fix the dispensing seals, ensure the installation stability of the dispensing seals, and thus improve the sealing effect.

[0105] Optionally, the sampling surface 113 is provided with collection avoidance grooves corresponding to the plurality of collection seals, and the collection seals are embedded in the collection avoidance grooves, so as to fix the collection seals, ensure the installation stability of the collection seals, and thus improve the sealing effect.

[0106] In this embodiment, the dispensing avoidance grooves and the collection avoidance grooves communicate with each other to form an integral seal avoidance groove 1133.

[0107] Optionally, as Figure 2As shown, the sample injection device further includes a temperature adjusting member 15, which is used to adjust the temperature of the reagent in the dispensing chamber 111 and / or the dispensing flow channel 1131, so as to meet the preparation requirements of different products.

[0108] Optionally, the sample injection member 11 is further provided with a heat preservation member 16. The heat preservation member 16 is sleeved outside the sample injection member 11, and the sample injection surface 113 is located outside the heat preservation member 16. Thus, the heat preservation effect of the sample injection member 11 is improved through the heat preservation member 16, the temperature stability of the reagent is ensured, and the heat preservation member 16 will not interfere with the microfluidic chip 2.

[0109] Exemplarily, the temperature adjusting member 15 includes an electric heating plate. The electric heating plate is embedded in the sample injection member 11, and the dispensing chamber 111 and the plurality of dispensing flow channels 1131 are correspondingly arranged with the electric heating plate. Thus, the electric heating plate can heat the reagent in the dispensing chamber 111 and the plurality of dispensing flow channels 1131 simultaneously, and precise temperature control of the reagent can be achieved.

[0110] Further, the heat preservation member 16 is a shell-like structure with one end open and one end closed. The heat preservation member 16 is sleeved outside the sample injection member 11, and the sample injection surface 113 and the open end are on the same side of the sample injection member 11. Thus, it will not interfere with the cooperation between the sample injection surface 113 and the microfluidic chip 2.

[0111] The microfluidic system of this embodiment includes at least one microfluidic unit arranged in parallel. The microfluidic unit includes a microfluidic chip 2 and at least one sample injection device as described above. The microfluidic chip 2 is arranged on the sample injection surface 113. The microfluidic chip 2 includes a plurality of channel units corresponding to the plurality of dispensing flow channels 1131 of the sample injection device. The channel unit includes a plurality of microfluidic channels 211 arranged at intervals along the third direction and isolated from each other. The dispensing flow channel 1131 communicates with the plurality of microfluidic channels 211 of the corresponding channel unit.

[0112] The microfluidic system of this embodiment, by applying the above sample injection device, not only improves the production capacity of the microfluidic system, but also enables the particle size of the products produced by the microfluidic system to be more uniform.

[0113] Figure 10 It is a schematic cross-sectional view of the microfluidic channel 211. The cross-section of the microfluidic channel 211 is perpendicular to the axis of the microfluidic channel 211. As Figure 10 shown, along the third direction, the length of the dispensing flow channel 1131 between two adjacent microfluidic channels 211 is L d . Along the axis of the microfluidic channel 211, the length of the microfluidic channel 211 is L b . The width of the microfluidic channel 211 is W b . Along the second direction, the depth of the microfluidic channel 211 is H b . Along the axis of the microfluidic channel 211, H bRemain unchanged, W b Remain unchanged. The second direction, the width direction of the microfluidic channel 211, and the axial direction of the microfluidic channel 211 are perpendicular to each other in pairs.

[0114] Furthermore, . By making , the distribution flow channel 1131 can evenly distribute the reagent to a plurality of microfluidic channels 211, and the flow rates of the reagents in each microfluidic channel 211 are the same, so that the particle size of the products prepared by the microfluidic system is more uniform. It should be noted that the cross-section of the microfluidic channel 211 is rectangular; the cross-section of the microfluidic channel 211 is perpendicular to the axial direction of the microfluidic channel 211. Exemplarily, the axis of the microfluidic channel 211 is a curve. Of course, in other embodiments, the axis of the microfluidic channel 211 can also be a straight line, which is not limited herein.

[0115] Optionally, as Figure 1 shown, there are two sampling devices. The microfluidic chip 2 includes two connection surfaces, and the two connection surfaces are respectively connected to the sampling surfaces 113 of the two sampling devices; the microfluidic channel 211 includes a microfluidic outlet 2112 and two microfluidic inlets 2111. The two microfluidic inlets 2111 are respectively located on the two connection surfaces, and the microfluidic outlet 2112 is located on one of the connection surfaces; in the same channel unit, the two microfluidic inlets 2111 of a plurality of microfluidic channels 211 are respectively communicated with the corresponding distribution flow channels 1131 of the two sampling devices, and the microfluidic outlets 2112 of the plurality of microfluidic channels 211 are all used for communicating with the collection device. It can be understood that the microfluidic chip 2 is clamped between the two sampling devices. By introducing two different reagents into the microfluidic channel 211 through the two sampling devices respectively, the flow rates of the two reagents in the microfluidic channel 211 can be made the same, so that products with uniform particle size can be prepared, and the prepared products are then discharged from the microfluidic outlet 2112 and centrally collected by the collection device.

[0116] Optionally, the sampling surface 113 of one of the sampling devices is provided with the above-mentioned collection flow channel 1132, that is, only the sampling surface 113 of one sampling device is provided with the collection flow channel 1132, and the sampling surface 113 of the other sampling device does not need to be provided with the collection flow channel 1132, which is convenient for centrally collecting the products prepared by the microfluidic system.

[0117] Optionally, a plurality of dispensing channels 1131 are arranged at equal intervals in the first direction, thereby improving the processability of the sample introduction member 11 and facilitating the machining of the dispensing channels 1131. Of course, the plurality of dispensing channels 1131 can also be divided into multiple groups, and the multiple groups of dispensing channels 1131 are arranged at equal intervals in the first direction. Each group of dispensing channels 1131 includes a plurality of dispensing channels 1131 arranged at equal intervals in the first direction. In this embodiment, neither the spacing between two adjacent groups of dispensing channels 1131 nor the spacing between two adjacent dispensing channels 1131 is limited.

[0118] Optionally, a plurality of collection channels 1132 are arranged at equal intervals in the first direction. Of course, the plurality of collection channels 1132 can also be divided into multiple groups, and the multiple groups of collection channels 1132 are arranged at equal intervals in the first direction. Each group of collection channels 1132 includes a plurality of collection channels 1132 arranged at equal intervals in the first direction. In this embodiment, neither the spacing between two adjacent groups of collection channels 1132 nor the spacing between two adjacent collection channels 1132 is limited.

[0119] It should be noted that the dispensing channels 1131 and the channel units can be arranged in one-to-one correspondence. That is to say, one dispensing channel 1131 is connected to a plurality of microfluidic channels 211 in a corresponding channel unit, and thus a reagent is dispensed into the plurality of microfluidic channels 211 in the corresponding channel unit through one dispensing channel 1131. Of course, one dispensing channel 1131 can also correspond to multiple channel units. That is to say, one dispensing channel 1131 is connected to a plurality of microfluidic channels 211 in the corresponding multiple channel units, and thus a reagent is dispensed into the plurality of microfluidic channels 211 in the corresponding multiple channel units through one dispensing channel 1131.

[0120] Furthermore, one collection channel 1132 is arranged in correspondence with one or more channel units. In other words, one collection channel 1132 is connected to a plurality of microfluidic outlets 2112 in a corresponding channel unit, and thus the product formed by one channel unit is discharged into the corresponding collection channel 1132; or, one collection channel 1132 is connected to a plurality of microfluidic outlets 2112 in multiple channel units, and thus the products formed by the multiple channel units are all discharged into the corresponding collection channel 1132. All the collection channels 1132 are connected to a collection device. The products discharged from the plurality of microfluidic channels 211 in the corresponding channel unit are preliminarily collected through the collection channels 1132, and then the collected products are transported to the collection device, improving the convenience of product collection.

[0121] Specifically, on the same connection surface, the microfluidic inlets 2111 of the multiple microfluidic channels 211 of one channel unit are arranged at equal intervals in the third direction to form a row of microfluidic inlets 2111. On the same connection surface, the microfluidic outlets 2112 of the multiple microfluidic channels 211 of one channel unit are arranged at equal intervals in the third direction to form a row of microfluidic outlets 2112. Of course, on the same connection surface, the microfluidic inlets 2111 of the multiple microfluidic channels 211 of two or more channel units can also be arranged at equal intervals in the third direction to form a row of microfluidic inlets 2111.

[0122] Furthermore, one distribution channel 1131 communicates with one row or multiple rows of microfluidic channels 211. One collection channel 1132 communicates with one row or multiple rows of microfluidic outlets 2112.

[0123] It should be noted that for the convenience of description, the two microfluidic inlets 2111 of the microfluidic channel 211 are respectively denoted as the first inlet and the second inlet, and the second inlet and the microfluidic outlet 2112 are located on the same connection surface. When L b is the length of the microfluidic channel 211 between the second inlet and the microfluidic outlet 2112, L d is the length of the distribution channel 1131 between two adjacent second inlets in the third direction. Specifically, the length of the distribution channel 1131 between two adjacent second inlets in the third direction is the distance between the axes of two adjacent second inlets in the third direction. When L b is the length of the microfluidic channel 211 between the first inlet and the microfluidic outlet 2112, L d is the length of the distribution channel 1131 between two adjacent first inlets in the third direction. Specifically, the length of the distribution channel 1131 between two adjacent first inlets in the third direction is the distance between the axes of two adjacent first inlets in the third direction.

[0124] As Figure 1 shown, in a specific embodiment of the present invention, the microfluidic system includes one microfluidic unit. The microfluidic unit includes a microfluidic chip 2 and two sampling devices, which are respectively denoted as the first sampling device 1a and the second sampling device 1b. The first sampling device 1a is used to distribute reagent A to the microfluidic chip 2, and the second sampling device 1b is used to distribute reagent B to the microfluidic chip body. The two connection surfaces of the microfluidic chip 2 are respectively denoted as the first connection surface 221 and the second connection surface 222. The first connection surface 221 and the second connection surface 222 are respectively located on both sides of the thickness direction of the microfluidic chip 2. The first connection surface 221 is connected to the sampling surface 113 of the first sampling device 1a, and the second connection surface 222 is connected to the sampling surface 113 of the second sampling device 1b.

[0125] Further, the microfluidic chip 2 includes twelve channel units, each channel unit includes twenty-five microfluidic channels 211, and each microfluidic channel 211 includes two microfluidic inlets 2111 and one microfluidic outlet 2112. One of the microfluidic inlets 2111 is located on the first connection surface 221 and is used to introduce reagent A, and the other microfluidic inlet 2111 is located on the second connection surface 222 and is used to introduce reagent B. The microfluidic outlet 2112 is located on the second connection surface 222 and is used to discharge the product formed in the microfluidic channel 211.

[0126] On the first connection surface 221, the twenty-five microfluidic inlets 2111 of one channel unit are arranged at equal intervals along the third direction to form a row of microfluidic inlets 2111.

[0127] On the second connection surface 222, the fifty microfluidic inlets 2111 of two channel units are arranged at equal intervals along the third direction to form a row of microfluidic inlets 2111, and the twenty-five microfluidic outlets 2112 of one channel unit are arranged at equal intervals along the third direction to form a row of microfluidic outlets 2112.

[0128] Further, for the first sampling device 1a, the first sampling device 1a is provided with twelve distribution channels 1131, and the twelve distribution channels 1131 are arranged in one-to-one correspondence with and communicated with the twelve rows of microfluidic inlets 2111 on the first connection surface 221. Reagent A is introduced into the distribution chamber 111 through the sampling port 112. After the distribution chamber 111 is pre-filled with reagent A, it is evenly distributed to the twelve distribution channels 1131, and then reagent A is distributed from each distribution channel 1131 to the corresponding microfluidic channel 211.

[0129] For the second sampling device 1b, the second sampling device 1b is provided with six distribution channels 1131 and seven collection channels 1132. The six distribution channels 1131 are arranged in one-to-one correspondence with and communicated with the six rows of microfluidic inlets 2111 on the second connection surface 222. Among the seven collection channels 1132, two of the collection channels 1132 are arranged in one-to-one correspondence with and communicated with two rows of microfluidic outlets 2112 on the second connection surface 222. Among the remaining five collection channels 1132 and the remaining ten rows of microfluidic outlets 2112, one collection channel 1132 is arranged in correspondence with two rows of microfluidic outlets 2112. Reagent B is introduced into the distribution chamber 111 through the sampling port 112. After the distribution chamber 111 is pre-filled with reagent B, it is evenly distributed to the six distribution channels 1131, and then reagent A is distributed from each distribution channel 1131 to the corresponding microfluidic channel 211.

[0130] Then, the product formed by reagent A and reagent B on the microfluidic chip 2 is discharged from the microfluidic outlet 2112 to the corresponding collection channel 1132, and then enters the collection device through the collection channel 1132.

[0131] In summary, the sample injection device of this embodiment realizes the parallel operation of 300 microfluidic channels 211 on the premise of not increasing the number of sample injection ports 112 and adopting a single-stage liquid separation method. Moreover, the flow rates of reagent A and reagent B in the 300 microfluidic channels 211 are consistent, and the product particle sizes prepared are uniform.

[0132] Table 1 shows the result comparison of different specifications of sample injection devices for droplet preparation

[0133]

[0134] As can be seen from Table 1, when at this time, as Figure 17 and Figure 18 shown, the sizes of the product droplets formed by the microfluidic channels 211 are uniform, indicating that the sample injection flow rates of the microfluidic channels 211 are uniform. When at this time, as Figure 16 shown, the product droplets formed by the microfluidic channels 211 are of different sizes, indicating that the sample injection flow rates between different microfluidic channels 211 are not uniform.

[0135] The microfluidic system of this embodiment can be used for high-throughput preparation of gelatin microspheres. The gelatin solution is in a solution state at an environmental temperature of 50°C - 60°C and turns into a solid when the temperature drops below room temperature. Therefore, a temperature adjustment member 15 needs to be embedded in the sample injection member 11 of the sample injection device to control the temperature of the gelatin solution through the temperature adjustment member 15. Further, a heat preservation member 16 is arranged outside the sample injection member 11. After the temperature of the gelatin solution is adjusted by the temperature adjustment member 15, the heat preservation member 16 can ensure the temperature stability of the gelatin droplets during the entire preparation process.

[0136] Exemplarily, the process of high-throughput preparation of gelatin microspheres using the microfluidic system of this embodiment is as follows:

[0137] 1. Adjust the temperature of the temperature adjustment member 15 to 60°C and start the preparation after stabilizing for 30 minutes.

[0138] 2. The first sample injection device 1a is used to distribute the gelatin solution to the microfluidic chip 2. The gelatin solution enters the distribution cavity 111 from the sample injection port 112 of the first sample injection device 1a. After pre-filling the distribution cavity 111, it is evenly distributed to twelve distribution channels 1131, and then the gelatin solution is evenly distributed into multiple microfluidic channels 211 of the microfluidic chip 2 through each distribution channel 1131.

[0139] 3. The first sample injection device 1a is used to distribute surfactant-containing mineral oil to the microfluidic chip 2. The surfactant-containing mineral oil enters the distribution chamber 111 from the sample injection port 112 of the second sample injection device 1b. After the distribution chamber 111 is pre-filled, it is evenly distributed to six distribution channels 1131, and then the surfactant-containing mineral oil is evenly distributed into a plurality of microfluidic channels 211 of the microfluidic chip 2 through each distribution channel 1131.

[0140] 4. On the microfluidic chip 2, the surfactant-containing mineral oil shears the gelatin solution to form gelatin droplets, and then the gelatin droplets are discharged from the microfluidic outlet 2112 and collected in the collection channel 1132, and then enter the low-temperature collection pool through the collection joint 13 for on-line curing to make gelatin microspheres.

[0141] In summary, by using the microfluidic system of this embodiment, the preparation throughput of gelatin microspheres can be increased from 2 μL / min - 5 μL / min to 600 μL / min - 1500 μL / min, and the particle size of the gelatin microspheres can be ensured to be highly uniform.

[0142] Embodiment Two

[0143] As Figures 12 - 15 shown, the difference between this embodiment and Embodiment One is that the microfluidic system is provided with a plurality of microfluidic units. The sample injection parts 11 of the first sample injection devices 1a of the plurality of microfluidic units are connected and form a total sample injection part 11a, and the distribution chambers 111 of the first sample injection devices 1a of the plurality of microfluidic units communicate and form a total distribution chamber 111a.

[0144] For the microfluidic system of this embodiment, reagents can be distributed to the distribution channels 1131 of the first sample injection devices 1a of a plurality of microfluidic units through a total distribution chamber 111a at the same time, which can not only further simplify the structure of the sample injection device, but also help to improve the consistency of the reagent flow rates in the distribution channels 1131 of the first sample injection devices 1a of the plurality of microfluidic units, and further make the particle size of the products prepared by the microfluidic system more uniform.

[0145] Further, as Figures 12 - 14 shown, the total sample injection part 11a includes a plurality of sample injection surfaces 113, and the plurality of sample injection surfaces 113 are sequentially arranged on the outer peripheral side of the total sample injection part 11a along the first circumferential direction, which is convenient for the assembly of the sample injection surface 113 and the corresponding microfluidic chip 2 and improves the assembly convenience.

[0146] It can be understood that the plurality of distribution channels 1131 on each sample injection surface 113 of the total sample injection part 11a communicate with the total distribution chamber 111a, and the sample injection port 112 communicates with the total distribution chamber 111a. The reagents introduced into the total distribution chamber 111a through one sample injection port 112 can uniformly enter the plurality of distribution channels 1131 on each sample injection surface 113.

[0147] Optionally, the total distribution cavity 111a includes a first distribution sub-cavity 111a1 and a second distribution sub-cavity 111a2 provided in one-to-one correspondence with a plurality of injection surfaces 113 of the total injection member 11a. The injection port 112 and the plurality of second distribution sub-cavities 111a2 are both communicated with the first distribution sub-cavity 111a1, and the plurality of second distribution sub-cavities 111a2 are sequentially communicated along the first circumferential direction to form a total distribution sub-cavity in an annular structure. That is to say, one injection port 112 and one first distribution sub-cavity 111a1 are provided, so that the reagent is introduced into the first distribution sub-cavity 111a1 through the single injection port 112, and then into the total distribution cavity through the first distribution sub-cavity 111a1, which is beneficial to improving the uniformity of reagent distribution.

[0148] Optionally, the total injection member 11a includes a first housing 114a and a second housing 114b. The plurality of injection surfaces 113 are sequentially provided on the outer peripheral side of the first housing 114a along the first circumferential direction; the second housing 114b is provided in the first housing 114a, and a total distribution cavity 111a is formed between the first housing 114a and the second housing 114b, which can reduce the manufacturing difficulty of the total injection member 11a and facilitate the assembly of the microfluidic system.

[0149] Optionally, along the third direction, one end of the second housing 114b abuts against the inner wall of the first housing 114a, and a first distribution sub-cavity 111a1 is formed between the other end of the second housing 114b and the inner wall of the first housing 114a. A total distribution sub-cavity is formed between the outer peripheral side of the second housing 114b around the third direction and the inner wall of the first housing 114a. Since the reagent has a certain pressure when being introduced into the total distribution cavity 111a from the injection port 112, with the above settings of the first housing 114a and the second housing 114b, it is not only convenient for the reagent to be introduced into the distribution flow channels 1131 on each injection surface 113, but also can avoid pressure loss of the reagent, having an energy-saving effect.

[0150] Optionally, the first housing 114a includes a shell portion 114a1 with two open ends and two cover portions 114a2. The two cover portions 114a2 are respectively covered on the two open ends, which is convenient for the assembly of the first housing 114a and the second housing 114b.

[0151] Optionally, a shell seal 114a3 is provided between the cover portion 114a2 and the shell portion 114a1 to improve the sealing performance.

[0152] In a specific embodiment of the present invention, as Figure 14As shown, the total sample injection part 11a includes four sample injection surfaces 113. The total sample injection part 11a has a cube structure, and the four sample injection surfaces 113 are respectively the four outer surfaces of the cube structure that are sequentially connected along its circumference. Preferably, the first distribution sub-chamber 111a1 is located above the second housing 114b and communicates with the total distribution sub-chamber. The total distribution sub-chamber has an annular structure and surrounds the second housing 114b on all sides.

[0153] In other embodiments, the number of sample injection surfaces 113 of the total sample injection part 11a can also be set to two, three, five, or even more, and can be selected according to the number of microfluidic chips 2, which is not limited herein.

[0154] The working principle of the microfluidic system in this embodiment is as follows:

[0155] For the total sample injection part 11a, reagent A is sequentially introduced into the first distribution sub-chamber 111a1 and the four second distribution sub-chambers 111a2 through the sample injection port 112. After reagent A pre-fills the total distribution chamber 111a, it is then evenly distributed to the twelve distribution channels 1131 on the four sample injection surfaces 113, and then reagent A is further distributed from each distribution channel 1131 to the corresponding microfluidic channel 211.

[0156] For each second sample injection device 1b, reagent B is introduced into the distribution chamber 111 through the sample injection port 112. After reagent B pre-fills the distribution chamber 111, it is then evenly distributed to the six distribution channels 1131, and then reagent A is further distributed from each distribution channel 1131 to the corresponding microfluidic channel 211.

[0157] Then, the products formed by reagent A and reagent B on the microfluidic chip 2 are discharged to the corresponding collection channels 1132 through the microfluidic outlet 2112, and then enter the collection device through the collection channels 1132.

[0158] In summary, the microfluidic system of this embodiment realizes the parallel operation of four microfluidic chips 2 on the premise of not increasing the number of sample injection ports 112 and adopting a single-stage liquid separation method. Each microfluidic chip 2 includes three hundred microfluidic channels 211, and the flow rates of reagent A and reagent B in the three hundred microfluidic channels 211 are consistent, and the particle sizes of the prepared products are uniform.

[0159] Exemplarily, the process of high-throughput preparation of nano-liposome drugs using the microfluidic system of this embodiment is as follows:

[0160] 1. Preparation of lipid solution: Accurately weigh 0.062 g of DSPC (distearoylphosphatidylcholine) and 0.0152 g of cholesterol, dissolve them in 39.45 g of ethanol, and perform ultrasonic dissolution. Filter the above mixed solution through a 0.22 μm organic filter membrane. Preparation of aqueous solution: Take 10 mL of 10×PBS solution (phosphate buffer solution), dilute it to 100 mL of 1×PBS solution with pure water, and then filter it through a 0.22 μm aqueous filter membrane.

[0161] 2. The lipid solution enters the total distribution chamber 111a from the injection port 112 of the total injection part 11a. After the lipid solution pre-fills the total distribution chamber 111a, it is evenly distributed to the distribution channels 1131 on each injection surface 113, and then is distributed to the corresponding microfluidic channels 211 through the respective distribution channels 1131.

[0162] 3. The aqueous solution enters the corresponding distribution chamber 111 from the injection ports 112 of the four second injection devices 1b respectively. After the aqueous solution pre-fills the distribution chamber 111, it is evenly distributed to six distribution channels 1131, and then the aqueous solution is distributed to the corresponding microfluidic channels 211 through each distribution channel 1131.

[0163] 4. The lipid solution and the aqueous solution are mixed in the microfluidic channel 211 and self-assembled to form nano-liposomes.

[0164] In summary, by using the microfluidic system of this embodiment, four microfluidic chips 2 can be used to simultaneously prepare nano-liposome drugs, and the preparation throughput of nano-liposome drugs can be increased from the laboratory level (100 mL / min) to the pilot scale level (400 mL / min).

[0165] Note that the above is only the preferred embodiment of the present invention and the technical principles applied. Those skilled in the art will understand that the present invention is not limited to the specific embodiments described herein. Various obvious changes, re-adjustments and substitutions can be made by those skilled in the art without departing from the protection scope of the present invention. Therefore, although the present invention has been described in detail through the above embodiments, the present invention is not limited to the above embodiments. Without departing from the concept of the present invention, more other equivalent embodiments can be included, and the scope of the present invention is determined by the scope of the appended claims.

Claims

1. Sampling device, characterized in that, Comprising: A sample introduction part, the sample introduction part includes a distribution cavity and a sample introduction port communicated with the distribution cavity. An outer side of the sample introduction part is provided with a sample introduction surface, and the sample introduction surface is provided with a plurality of distribution channels all communicated with the distribution cavity. The plurality of distribution channels are arranged at intervals along a first direction; In the first direction, the length of the distribution cavity between two adjacent distribution channels is l d , and the width of the distribution channel is w b ; In the second direction, the width of the dispensing cavity is w d , and the depth of the dispensing flow channel is h b ; In the third direction, the depth of the distribution cavity is h d , and the length of the distribution flow channel is l b ; Along the first direction, h d remains unchanged, w d remains unchanged; Along the third direction, h b remains unchanged, w b remains unchanged; ； The first direction, the second direction and the third direction are arranged at an angle to each other in pairs.

2. The sample introduction device according to claim 1, characterized in that, The sample introduction part includes a housing and a cover body. The sample introduction surface is arranged on the housing, and the housing or the cover body is provided with a groove. The cover body is connected with the housing to seal the notch of the groove and form the distribution cavity.

3. The sample introduction device according to claim 2, characterized in that, The housing is further provided with connection channels respectively corresponding to the plurality of distribution channels, and the distribution channels are communicated with the distribution cavity through the corresponding connection channels.

4. The sample injection device according to claim 2, wherein, The distribution cavity and the distribution channels are respectively located on two sides of the housing.

5. The sample introduction device according to claim 1, characterized in that, The sample introduction surface is further provided with a collection channel for communicating with a collection device, and the distribution cavity and the distribution channels are isolated from each other with respect to the collection channel.

6. The sample injection device according to claim 5, characterized in that, The sample introduction device further includes distribution seals respectively corresponding to the plurality of distribution channels, and the distribution seals are sleeved on outer sides of the corresponding distribution channels; The sample introduction device further includes collection seals respectively corresponding to the plurality of collection channels, and the collection seals are sleeved on outer sides of the corresponding collection channels.

7. The sample introduction device according to claim 6, characterized in that, The plurality of distribution seals are integrally formed; or, the plurality of distribution seals are separately arranged and fixedly connected; The plurality of collection seals are integrally formed; or, the plurality of collection seals are separately arranged and fixedly connected.

8. The sample introduction device according to claim 6, characterized in that, The sample introduction surface is provided with distribution avoidance grooves corresponding to the plurality of distribution seals, and the distribution seals are embedded in the distribution avoidance grooves; The sample introduction surface is provided with collection avoidance grooves corresponding to the plurality of collection seals, and the collection seals are embedded in the collection avoidance grooves.

9. The sampling device according to any one of claims 1-8, characterized in that, The sample introduction device further includes a temperature regulating part for regulating the temperature of a reagent in the distribution cavity and / or the distribution channels; and / or, The sample introduction device further includes a heat preservation part sleeved on an outer side of the sample introduction part, and the sample introduction surface is located outside the heat preservation part.

10. The sampling device according to any one of claims 1-8, characterized in that, The first direction, the second direction and the third direction are perpendicular to each other in pairs.

11. A microfluidic system, characterized in that, Including at least one microfluidic unit arranged in parallel. The microfluidic unit includes a microfluidic chip and at least one sample introduction device as described in any one of claims 1-10. The microfluidic chip is arranged on the sample introduction surface. The microfluidic chip includes a plurality of channel units corresponding to the plurality of distribution channels of the sample introduction device. The channel unit includes a plurality of microfluidic channels arranged at intervals along the third direction and isolated from each other, and the distribution channels are communicated with the plurality of microfluidic channels of the corresponding channel unit.

12. The microfluidic system according to claim 11, wherein In the third direction, the length of the dispensing flow channel located between two adjacent microfluidic channels is L d ; Along the axial direction of the microfluidic channel, the length of the microfluidic channel is L b ; The width of the microfluidic channel is W b ; Along the second direction, the depth of the microfluidic channel is H b ; Axially along the microfluidic channel, H b remains unchanged, W b remains unchanged; ； The second direction, the width direction of the microfluidic channel and the axial direction of the microfluidic channel are perpendicular to each other in pairs.

13. The microfluidic system according to claim 12, wherein, There are two sample introduction devices, and the microfluidic chip includes two connection surfaces, and the two connection surfaces are respectively connected with the sample introduction surfaces of the two sample introduction devices; The microfluidic channel includes a microfluidic outlet and two microfluidic inlets, the two microfluidic inlets are respectively located on the two connection surfaces, and the microfluidic outlet is located on one of the connection surfaces; In the same channel unit, the two microfluidic inlets of multiple microfluidic channels are respectively communicated with the corresponding distribution channels of the two sampling devices, and the microfluidic outlets of the multiple microfluidic channels are all used for communicating with the collection device.

14. The microfluidic system according to claim 13, wherein At least one collection channel corresponding to multiple channel units is further provided on the sampling surface of one of the sampling devices, the microfluidic outlets of the multiple channel units are all communicated with the corresponding collection channel, and the collection channel is communicated with the collection device.

15. The microfluidic system according to claim 12, wherein The two sampling devices of the same microfluidic unit are respectively a first sampling device and a second sampling device; There are multiple microfluidic units, the sampling parts of the first sampling devices of the multiple microfluidic units are connected to form a total sampling part, and the distribution cavities of the first sampling devices of the multiple microfluidic units are communicated to form a total distribution cavity.

16. The microfluidic system according to claim 15, wherein, The total sampling part includes multiple sampling surfaces, and the multiple sampling surfaces are sequentially arranged on the outer peripheral side of the total sampling part along a first circumferential direction.

17. The microfluidic system according to claim 16, wherein, The total distribution cavity includes a first distribution sub-cavity and second distribution sub-cavities corresponding one by one to the multiple sampling surfaces of the total sampling part. The sampling port and the multiple second distribution sub-cavities are all communicated with the first distribution sub-cavity, and the multiple second distribution sub-cavities are sequentially communicated along the first circumferential direction to form a total distribution sub-cavity with an annular structure.

18. The microfluidic system according to claim 17, characterized in that, The total sampling part includes: A first housing, multiple sampling surfaces are sequentially arranged on the outer peripheral side of the first housing along the first circumferential direction; A second housing, the second housing is arranged inside the first housing, and the total distribution cavity is formed between the first housing and the second housing.

19. The microfluidic system according to claim 18, characterized in that, Along the third direction, one end of the second housing abuts against the inner wall of the first housing, a first distribution sub-cavity is formed between the other end of the second housing and the inner wall of the first housing, and a total distribution sub-cavity is formed between the outer peripheral side of the second housing around the third direction and the inner wall of the first housing.

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