Tumor cell culture device capable of accurately controlling temperature

Abstract

The invention provides a tumor cell culture device capable of accurately controlling temperature, and relates to the field of cell biological culture devices.The tumor cell culture device comprises a carbon dioxide incubator body, the left side and the right side of the interior of the carbon dioxide incubator body are each provided with two temperature adjusting mechanisms, and a demisting mechanism is installed on the front side of the upper end of the interior of the carbon dioxide incubator body. Through the arrangement of the demisting mechanism, after the sealing box door is opened, a door control switch triggers a controller to start an electric heating pipe fitting B and an air blower, heated air is blown downwards to the rear surface of the glass door through evenly-distributed air blowing pipes, rapid demisting is achieved, normal observation of experimenters is prevented from being hindered, and the operation complexity and the pollution risk are effectively reduced; the problems that in the using process of an existing carbon dioxide incubator, a glass door needs to be opened to be cleaned when defogging operation is conducted on the glass door, the constant-temperature and constant-humidity state of the culture environment is damaged, and operation complexity and pollution risks are increased are solved.

Description

Technical Field

[0001] The present invention relates to the technical field of cell biological culture devices, and particularly to a tumor cell culture device with precisely controllable temperature. Background Art

[0002] In vitro culture of tumor cells is an important technical means for basic cancer research, drug screening, and personalized treatment. Currently, in the process of tumor cell culture, microbial culture instruments such as carbon dioxide incubators are generally used as the core equipment. The carbon dioxide incubator realizes the in vitro culture of tumor cells by simulating a growth environment similar to that in the living body inside it. The carbon dioxide incubator is widely used not only for tumor cells but also for tissue culture and the culture of certain special microorganisms.

[0003] During the use of the existing carbon dioxide incubator, in order to maintain the stability of the culture environment, a relatively high humidity needs to be maintained inside the carbon dioxide incubator. When the humid air inside the incubator contacts the relatively low-temperature glass door, water vapor is likely to condense on the rear surface of the glass door to generate fog, seriously interfering with experimental observation. And the conventional defogging operation requires opening the glass door, which not only destroys the constant temperature and humidity state of the culture environment but also increases the operation complexity and pollution risk. In addition, although the existing carbon dioxide incubator adopts a double-door design (inner glass door + outer magnetic-sealed box door) to enhance airtightness, the magnetic force of the magnetic-sealed box door will decay after long-term use, resulting in a decrease in airtightness, thus affecting the stability of tumor cell culture. Summary of the Invention

[0004] The present invention relates to a tumor cell culture device with precisely controllable temperature. Through the setting of a defogging mechanism, when the sealed box door is opened, the door control switch triggers the controller to start the electric heating pipe fitting B and the hair dryer, and the heated air is blown downward through the uniformly distributed air blowing pipes to the rear surface of the glass door, realizing rapid defogging, avoiding interference with the normal observation of experimental personnel, and without the need to open the glass door for cleaning, effectively reducing the operation complexity and pollution risk; through the setting of a locking plug, a plug-in limiting component, a foot-operated mechanism, and a locking mechanism, after the sealed box door is closed, the upper end of the plug-in block can be automatically inserted into the rectangular socket to fix the sealed box door, effectively solving the pain point of easy attenuation of traditional magnetic sealing, and thus improving the stability of tumor cell culture.

[0005] In the first aspect of the present invention, a tumor cell culture device with precisely controllable temperature is provided, specifically including: a carbon dioxide incubator main body, on the front side of which a glass door and a sealed box door are rotatably connected through a rotating shaft; two temperature regulating mechanisms are arranged on both the left and right sides inside the carbon dioxide incubator main body, and a defogging mechanism is installed on the front side of the upper end inside the carbon dioxide incubator main body; a plug-in limiting component is installed on the front side of the left end face of the carbon dioxide incubator main body, a chassis is installed at the bottom of the carbon dioxide incubator main body, and a foot-operating mechanism is installed at the front part of the left side of the chassis; a locking mechanism is arranged on the plug-in limiting component; The defogging mechanism includes a blowing shell cover, which is installed on the front side of the upper end inside the carbon dioxide incubator main body, and blowing pipes are evenly arranged at the bottom of the blowing shell cover. The air inlet at the top of the blowing shell cover is connected to an air pipe that penetrates the left side wall of the carbon dioxide incubator main body, and the other end of the air pipe is connected to the air outlet of a blower. The blower is installed on the left end face of the carbon dioxide incubator main body. An electric heating pipe B is arranged inside the blowing shell cover; an antibacterial filter screen is installed on the air suction port of the blower.

[0006] Furthermore, a controller is installed on the left end face of the carbon dioxide incubator main body, and a door control switch is installed at the lower part of the front end face of the carbon dioxide incubator main body; a locking plug board is fixedly connected to the sealed box door, and a rectangular socket is opened on the upper end face of the locking plug board.

[0007] Furthermore, when the sealed box door is in the closed state, the inner side surface of the sealed box door is in close contact with the front end of the pressing rod on the door control switch.

[0008] Furthermore, the temperature regulating mechanism includes a blowing shell body, which is installed inside the carbon dioxide incubator main body, and a temperature sensor is arranged on the rear end face of the blowing shell body. The air inlet of the blowing shell body penetrates one side surface of the carbon dioxide incubator main body, and the outer end of the air inlet of the blowing shell body is connected to the air outlet of a temperature regulating blower. The temperature regulating blower is installed outside the carbon dioxide incubator main body. An electric heating pipe A is arranged inside the blowing shell body; an antibacterial filter screen A is installed on the air suction port of the temperature regulating blower.

[0009] Furthermore, the plug-in limiting component includes a rectangular sliding shell, which is installed on the front side of the left end face of the carbon dioxide incubator main body, and a sliding shell is slidably connected inside the rectangular sliding shell. A plug-in block that penetrates the top of the rectangular sliding shell is fixedly connected to the upper end of the sliding shell; two spring guide rods that penetrate the bottom of the rectangular sliding shell are fixedly connected to the lower end face of the sliding shell, and a spring is sleeved outside each spring guide rod inside the rectangular sliding shell; a limiting jack is opened at the upper part of the rear end face of the rectangular sliding shell; two pulleys are rotatably connected inside the sliding shell through a rotating shaft, and a limiting block is fixedly connected inside the sliding shell.

[0010] Further, when the plug-in limit member is in the limit state, the upper end of the plug-in block is plugged into the rectangular socket.

[0011] Further, the locking mechanism includes a sliding plate, on which two guide rods are slidably connected, and the two guide rods are fixedly connected to the upper side inside the sliding housing. Springs are sleeved on the outside of each guide rod in front of the sliding plate; a locking plug column penetrating the rear side wall of the sliding housing is fixedly connected to the rear end face of the sliding plate; the front end face of the sliding plate is fixedly connected to the upper end of the pull-down rope.

[0012] Further, the foot-operated mechanism includes a U-shaped support plate fixedly connected to the front part of the left side of the chassis. Four vertical guide rods are arranged inside the U-shaped support plate. A foot-operated block is slidably connected inside the U-shaped support plate through the four vertical guide rods. The upper end face of the foot-operated block is fixedly connected to a pull-down rope penetrating the top of the U-shaped support plate. The pull-down rope also penetrates the bottom of the sliding housing. The upper end of the pull-down rope is fixedly connected to the front end face of the sliding plate, and the pull-down rope is slidably connected to two pulleys.

[0013] Further, when the locking mechanism is in the locking and limiting state, the rear end of the locking plug column is plugged into the limiting socket.

[0014] Further, when the foot-operated block is stepped down to the middle position inside the U-shaped support plate, the locking plug column will be pulled out from the inside of the limiting socket, and at this time, the front end face of the sliding plate contacts the rear end face of the limiting block.

[0015] The present invention provides a tumor cell culture device with precisely controllable temperature, having the following beneficial effects: First, through the setting of the defogging mechanism, when the sealed box door is opened, the door control switch triggers the controller to start the electric heating pipe B and the hair dryer, and blows the heated air downward through the uniformly distributed air blowing pipes to the rear surface of the glass door, realizing rapid defogging, avoiding interfering with the normal observation of the experimenter, and without the need to open the glass door for cleaning, effectively reducing the operation complexity and pollution risk. Moreover, it can form a hot air barrier when the glass door is opened, reducing the entry of cold air outside the box; in addition, a defogging mechanism is added to the lower side inside the carbon dioxide incubator main body to blow hot air upward, which can further improve the defogging effect and reduce the amount of cold air entering when the door is opened.

[0016] Second, through the settings of the locking plug board, the plug-in limiting component, the foot-operated mechanism and the locking mechanism, after the sealed box door is closed, the foot can be moved away from the foot-operated block, and the upper end of the plug-in block automatically inserts into the rectangular socket, realizing the fixation of the sealed box door. Moreover, under the action of the spring force of the spring outside the guide rod, the sliding plate drives the locking plug post to move backward, so that the locking plug post inserts into the limiting socket, further locking and limiting the sliding housing and the plug-in block. Therefore, the reliability of the sealed box door in the closed and sealed state is greatly improved, effectively solving the pain point of the easy attenuation of the traditional magnetic adsorption type seal, and thus enhancing the stability of tumor cell culture.

[0017] Third, through the settings of the four temperature control mechanisms, when culturing tumor cells, the four temperature sensors can real-time monitor the temperatures at four positions inside the carbon dioxide incubator main body. Once the temperature at a certain place is too low, the controller will start the corresponding electric heating pipe fitting A and the temperature control fan, blowing the high-temperature air to the low-temperature place to ensure the uniform temperature inside the box, realizing precise temperature control, ensuring the normal proliferation of tumor cells and the stability of genes, improving the experimental repeatability, and avoiding experimental deviations caused by temperature stress.

[0018] Fourth, through the setting of the foot-operated mechanism, when it is necessary to pull out the plug-in block from the rectangular socket, only need to step on the foot-operated block downward with the foot, and the plug-in block can be driven to move downward to complete the pulling-out operation. Since there is no need for manual plugging and unplugging, the operation steps are reduced, and the convenience of this culture device in daily use is greatly improved. Brief Description of the Drawings

[0019] In order to more clearly illustrate the technical solutions of the present invention, the drawings of the present invention will be briefly introduced below.

[0020] In the drawings: Figure 1 The structural schematic diagram of the first perspective of the present application is shown; Figure 2 The structural schematic diagram of the second perspective of the present application is shown; Figure 3 The structural schematic diagram of the present application in the disassembled state is shown; Figure 4 The structural schematic diagram of the partial cross-section of the blowing housing of the present application is shown; Figure 5 The structural schematic diagram of the partial cross-section of the blowing housing cover of the present application is shown; Figure 6 The structural schematic diagram of the plug-in limiting component and the foot-operated mechanism of the present application after disassembly is shown; Figure 7 The structural schematic diagram of the partial cross-section of the sliding housing of the present application is shown; Figure 8The structural schematic diagram after the split of the sliding housing and the locking mechanism of the present application is shown.

[0021] List of reference numerals 1. Carbon dioxide incubator main body; 101. Glass door; 102. Sealed chamber door; 103. Locking plug board; 104. Rectangular socket; 105. Controller; 106. Underframe; 107. Door control switch; 2. Temperature regulating mechanism; 201. Blowing housing; 202. Temperature regulating fan; 203. Temperature sensor; 204. Antibacterial filter A; 205. Electric heating pipe fitting A; 3. Demisting mechanism; 301. Blowing housing cover; 302. Blowing pipe; 303. Blower; 304. Antibacterial filter B; 305. Electric heating pipe fitting B; 4. Plug-in limiting component; 401. Rectangular sliding housing; 402. Sliding housing; 403. Plug-in block; 404. Spring guide rod; 405. Limiting jack; 406. Pulley; 407. Limiting block; 5. Pedal mechanism; 501. C-shaped support plate; 502. Pedal block; 503. Pull-down rope; 6. Locking mechanism; 601. Sliding plate; 602. Locking plug post; 603. Guide rod. Detailed implementation manners

[0022] To make the objectives, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are some but not all of the embodiments of the present invention. All other embodiments obtained by those of ordinary skill in the art based on the described embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0023] Embodiment 1: Please refer to Figures 1 to 8 : The present invention provides a tumor cell culture device with precisely controllable temperature, including: a carbon dioxide incubator main body 1, a glass door 101 and a sealed chamber door 102 are rotatably connected to the front side of the carbon dioxide incubator main body 1 through a rotating shaft; two temperature regulating mechanisms 2 are arranged on both the left and right sides inside the carbon dioxide incubator main body 1, and a demisting mechanism 3 is installed on the front side of the upper end inside the carbon dioxide incubator main body 1; a plug-in limiting component 4 is installed on the front side of the left end face of the carbon dioxide incubator main body 1, an underframe 106 is installed at the bottom of the carbon dioxide incubator main body 1, and a pedal mechanism 5 is installed at the front part of the left side of the underframe 106; a locking mechanism 6 is arranged on the plug-in limiting component 4; The defogging mechanism 3 includes a blowing housing 301, which is installed at the front side of the upper end inside the main body 1 of the carbon dioxide incubator. The bottom of the blowing housing 301 is evenly provided with blowing pipes 302. The air inlet at the top of the blowing housing 301 is connected to an air pipe that penetrates the left side wall of the main body 1 of the carbon dioxide incubator, and the other end of the air pipe is connected to the air outlet of a blower 303. The blower 303 is installed on the left end face of the main body 1 of the carbon dioxide incubator. An electric heating component B 305 is arranged inside the blowing housing 301; an antibacterial filter screen B 304 is installed on the air suction port of the blower 303, which can effectively prevent microorganisms such as bacteria and fungi in the air from entering; through the setting of the defogging mechanism 3, when the sealed box door 102 is opened, the door control switch 107 triggers the controller 105 to start the electric heating component B 305 and the blower 303, and blows the heated air downward through the evenly distributed blowing pipes 302 to the rear surface of the glass door 101, realizing the function of rapid defogging and avoiding interfering with the normal observation of the experimenter.

[0024] A controller 105 is installed on the left end face of the main body 1 of the carbon dioxide incubator, and a door control switch 107 is installed at the lower part of the front end face of the main body 1 of the carbon dioxide incubator; a locking plug 103 is fixedly connected to the sealed box door 102, and a rectangular socket 104 is opened on the upper end face of the locking plug 103 for inserting with the inserting block 403.

[0025] When the sealed box door 102 is in the closed state, the inner side surface of the sealed box door 102 is in close contact with the front end of the pressing rod on the door control switch 107. At this time, the pressing rod on the door control switch 107 is in a pressed state.

[0026] The temperature regulating mechanism 2 includes a blowing housing 201, which is installed inside the main body 1 of the carbon dioxide incubator. A temperature sensor 203 is arranged on the rear end face of the blowing housing 201. The air inlet of the blowing housing 201 penetrates one side surface of the main body 1 of the carbon dioxide incubator, and the outer end of the air inlet of the blowing housing 201 is connected to the air outlet of a temperature regulating blower 202. The temperature regulating blower 202 is installed outside the main body 1 of the carbon dioxide incubator. An electric heating component A 205 is arranged inside the blowing housing 201; the temperature regulating blower 202, the electric heating component A 205, the electric heating component B 305, and the blower 303 are all electrically connected to the controller 105; an antibacterial filter screen A 204 is installed on the air suction port of the temperature regulating blower 202, which can effectively prevent microorganisms such as bacteria and fungi in the air from entering; through the setting of the four temperature regulating mechanisms 2, when culturing tumor cells, the four temperature sensors 203 can monitor the temperatures at four positions inside the main body 1 of the carbon dioxide incubator in real time. Once the temperature at a certain place is too low, the controller 105 will start the corresponding electric heating component A 205 and the temperature regulating blower 202, and blow the high-temperature air to the low-temperature place to ensure the uniform temperature inside the box and realize precise temperature control.

[0027] The plug-in limit component 4 includes a rectangular sliding shell 401 which is installed on the front side of the left end face of the carbon dioxide incubator main body 1. A sliding shell 402 is slidably connected inside the rectangular sliding shell 401. An insertion block 403 penetrating through the top of the rectangular sliding shell 401 is fixedly connected to the upper end of the sliding shell 402. Two spring guide rods 404 penetrating through the bottom of the rectangular sliding shell 401 are fixedly connected to the lower end face of the sliding shell 402, and springs are sleeved on the outer sides of each spring guide rod 404 inside the rectangular sliding shell 401. A limit insertion hole 405 is formed in the upper part of the rear end face of the rectangular sliding shell 401. Two pulleys 406 are rotatably connected inside the sliding shell 402 through a rotating shaft, and a limit block 407 is fixedly connected inside the sliding shell 402. Through the arrangement of the plug-in limit component 4, it is used to limit the sealing chamber door 102.

[0028] When the plug-in limit component 4 is in the limit state, the upper end of the insertion block 403 is inserted into the rectangular insertion hole 104. At this time, the sealing chamber door 102 can be effectively fixed.

[0029] Embodiment 2, on the basis of Embodiment 1, as Figures 6 to 8 shown, the locking mechanism 6 includes a sliding plate 601. Two guide rods 603 are slidably connected to the sliding plate 601, and the two guide rods 603 are fixedly connected to the upper side inside the sliding shell 402. Springs are sleeved on the outer sides of each guide rod 603 in front of the sliding plate 601. A locking plug post 602 penetrating through the rear side wall of the sliding shell 402 is fixedly connected to the rear end face of the sliding plate 601. The front end face of the sliding plate 601 is fixedly connected to the upper end of the pull-down rope 503. Through the arrangement of the locking mechanism 6, the sliding plate 601 can drive the locking plug post 602 to move backward under the elastic force of the spring outside the guide rod 603, so that the locking plug post 602 is inserted into the limit insertion hole 405 to further lock and limit the sliding shell 402 and the insertion block 403, improving the reliability of the sealing chamber door 102 in the closed and sealed state.

[0030] The foot-operated mechanism 5 includes a U-shaped support plate 501. The U-shaped support plate 501 is fixedly connected to the front part of the left side of the chassis 106, and four vertical guide rods are arranged inside the U-shaped support plate 501. A foot-operated block 502 is slidably connected inside the U-shaped support plate 501 through the four vertical guide rods. The upper end surface of the foot-operated block 502 is fixedly connected to a downward pull rope 503 that penetrates the top of the U-shaped support plate 501. The downward pull rope 503 also penetrates the bottom of the sliding housing 402. The upper end of the downward pull rope 503 is fixedly connected to the front end surface of the sliding plate 601, and the downward pull rope 503 is slidably connected to two pulleys 406. Through the setting of the foot-operated mechanism 5, when it is necessary to pull out the insertion block 403 from the rectangular socket 104, only by stepping on the foot-operated block 502 downward with the foot, the insertion block 403 can be moved downward to complete the pulling-out operation. Since there is no need for manual insertion and extraction, the operation steps are reduced, and the convenience of the present culture device in daily use is improved.

[0031] When the locking mechanism 6 is in the locked and limited position, the rear end of the locking plug 602 is inserted into the limit socket 405. At this time, the sliding housing 402 and the insertion block 403 can be further locked and limited.

[0032] When the foot-operated block 502 is stepped on downward to the middle position inside the U-shaped support plate 501, the locking plug 602 will be pulled out from the limit socket 405. At this time, the front end surface of the sliding plate 601 contacts the rear end surface of the limit block 407. At this time, the limit block 407 limits the sliding plate 601.

[0033] The working principle of the present invention: When in use, when culturing tumor cells, first place the tumor cells inside the carbon dioxide incubator main body 1, then close the glass door 101, and then step on the foot-operated block 502 downward with the foot, driving the lower end of the downward pull rope 503 to move downward. Then, drive the sliding plate 601 and the locking plug 602 to move forward through the upper end of the downward pull rope 503, so that the locking plug 602 is pulled out from the limit socket 405. Then close the sealed box door 102, and then move the foot away from the foot-operated block 502. At this time, the sliding housing 402 drives the insertion block 403 to move upward under the spring force of the spring outside the spring guide rod 404, so that the upper end of the insertion block 403 is inserted into the rectangular socket 104 to fix the sealed box door 102. And the sliding plate 601 will drive the locking plug 602 to move backward under the spring force of the spring outside the guide rod 603, so that the locking plug 602 is inserted into the limit socket 405 to further lock and limit the sliding housing 402 and the insertion block 403. Therefore, the reliability of the sealed box door 102 in the closed and sealed state is greatly improved, and the phenomenon of reduced sealing performance due to magnetic force attenuation is avoided. Therefore, the stability of tumor cell culture is improved.

[0034] During the cultivation process of tumor cells inside the main body 1 of the carbon dioxide incubator, the specific temperatures at four different positions inside the main body 1 of the carbon dioxide incubator can be monitored in real time through four temperature sensors 203. When the temperature at a certain position is too low, the controller 105 will start a corresponding electric heating pipe fitting A205 and a corresponding temperature regulating fan 202. High temperature is generated by the corresponding electric heating pipe fitting A205, and then the corresponding temperature regulating fan 202 blows the hot air heated by the electric heating pipe fitting A205 to the position with too low temperature, realizing precise temperature control.

[0035] When the experimenter needs to observe the cultivation situation of tumor cells inside the main body 1 of the carbon dioxide incubator, first step on the foot-operated block 502 downward with the foot, drive the sliding plate 601 and the locking plug 602 to move forward through the downward pull rope 503. The locking plug 602 is first pulled out from the inside of the limit jack 405, and at this time, the front end surface of the sliding plate 601 contacts the rear end surface of the limit block 407. Then continue to step on the foot-operated block 502 downward, forcing the sliding plate 601 to drive the guide rod 603, the sliding housing 402 and the plug-in block 403 to move downward, and finally the plug-in block 403 is pulled out from the rectangular socket 104, and then open the sealed chamber door 102, and observe the cultivation situation of tumor cells inside the main body 1 of the carbon dioxide incubator through the glass door 101; and after the sealed chamber door 102 is opened, the pressing rod on the door control switch 107 loses the pushing effect. At this time, the controller 105 starts the electric heating pipe fitting B305 and the hair dryer 303. The electric heating pipe fitting B305 heats the air inside the blowing shell 301, and then the hair dryer 303 evenly blows the heated air inside the blowing shell 301 downward through the blowing pipe 302 to the rear (inner) surface of the glass door 101, performing a heating treatment on the rear surface of the glass door 101, so as to be able to remove the fog attached to the rear surface of the glass door 101 and avoid interfering with the normal observation of the experimenter. Another advantage of blowing hot air downward through the blowing pipe 302 is that when the glass door 101 is opened, blowing hot air downward through the blowing pipe 302 can form a hot air barrier, thereby being able to reduce the entry of cold air outside the box. Moreover, a defogging mechanism 3 can be installed on the lower side inside the main body 1 of the carbon dioxide incubator, and hot air is blown upward through the defogging mechanism 3 on the lower side to further improve the defogging effect on the rear surface of the glass door 101 and be able to further reduce the entry of cold air outside the box when the glass door 101 is opened.

[0036] In this article, the following points need to be noted: 1. The attached drawings of the embodiments of the present invention only relate to the structures involved in the embodiments of the present invention, and other structures can refer to the general design.

[0037] 2. Without conflict, the embodiments of the present invention and the features in the embodiments can be combined with each other to obtain new embodiments.

[0038] The above are only specific embodiments of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art within the technical scope disclosed by the present invention can easily think of changes or substitutions, which should all be covered within the protection scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.

Claims

1. A tumor cell culture device with precisely controllable temperature, comprising: Carbon dioxide incubator main body (1), a glass door (101) and a sealed box door (102) are rotatably connected to the front side of the carbon dioxide incubator main body (1) through a rotating shaft; characterized in that, two temperature adjusting mechanisms (2) are arranged on both the left and right sides inside the carbon dioxide incubator main body (1), and a defogging mechanism (3) is installed on the front side of the upper end inside the carbon dioxide incubator main body (1); a plug-in limiting component (4) is installed on the front side of the left end face of the carbon dioxide incubator main body (1), a bottom frame (106) is installed at the bottom of the carbon dioxide incubator main body (1), and a foot-operated mechanism (5) is installed at the front part of the left side of the bottom frame (106); a locking mechanism (6) is arranged on the plug-in limiting component (4). The defogging mechanism (3) includes a blowing shell cover (301), the blowing shell cover (301) is installed on the front side of the upper end inside the carbon dioxide incubator main body (1), and blowing pipes (302) are evenly arranged at the bottom of the blowing shell cover (301), an air inlet at the top of the blowing shell cover (301) is connected to an air pipe passing through the left side wall of the carbon dioxide incubator main body (1), and the other end of the air pipe is connected to the air outlet of a blower (303), the blower (303) is installed on the left end face of the carbon dioxide incubator main body (1), and an electric heating pipe part B (305) is arranged inside the blowing shell cover (301); a bacteriostatic filter screen (304) is installed on the air suction port of the blower (303).

2. The tumor cell culture device with precisely controllable temperature according to claim 1, characterized in that: A controller (105) is installed on the left end face of the carbon dioxide incubator main body (1), and a door control switch (107) is installed on the lower part of the front end face of the carbon dioxide incubator main body (1); a locking plug board (103) is fixedly connected to the sealed box door (102), and a rectangular socket (104) is opened on the upper end face of the locking plug board (103).

3. The tumor cell culture device with precisely controllable temperature according to claim 2, wherein: When the sealed box door (102) is in a closed state, the inner side surface of the sealed box door (102) is in close contact with the front end of the pressing rod on the door control switch (107).

4. The tumor cell culture device with precisely controllable temperature according to claim 1, characterized in that: The temperature adjusting mechanism (2) includes a blowing housing (201), the blowing housing (201) is installed inside the carbon dioxide incubator main body (1), a temperature sensor (203) is arranged on the rear end face of the blowing housing (201), the air inlet of the blowing housing (201) passes through one side surface of the carbon dioxide incubator main body (1), and the outer end of the air inlet of the blowing housing (201) is connected to the air outlet of a temperature adjusting blower (202), the temperature adjusting blower (202) is installed outside the carbon dioxide incubator main body (1), and an electric heating pipe part A (205) is arranged inside the blowing housing (201); a bacteriostatic filter screen A (204) is installed on the air suction port of the temperature adjusting blower (202).

5. The tumor cell culture device with precisely controllable temperature according to claim 2, characterized in that: The plug-in limiting component (4) includes a rectangular sliding shell (401). The rectangular sliding shell (401) is installed on the front side of the left end face of the carbon dioxide incubator main body (1), and a sliding shell (402) is slidably connected inside the rectangular sliding shell (401). A plug-in block (403) passing through the top of the rectangular sliding shell (401) is fixedly connected to the upper end of the sliding shell (402). Two spring guide rods (404) passing through the bottom of the rectangular sliding shell (401) are fixedly connected to the lower end face of the sliding shell (402), and a spring is sleeved on the outside of each spring guide rod (404) inside the rectangular sliding shell (401). A limiting jack (405) is opened in the upper part of the rear end face of the rectangular sliding shell (401). Two pulleys (406) are rotatably connected inside the sliding shell (402) through a rotating shaft, and a limiting block (407) is fixedly connected inside the sliding shell (402).

6. The tumor cell culture device with precisely controllable temperature according to claim 5, characterized in that: When the plug-in limiting component (4) is in the limiting state, the upper end of the plug-in block (403) is plugged into the rectangular socket (104).

7. The tumor cell culture device with precisely controllable temperature according to claim 5, characterized in that: The locking mechanism (6) includes a sliding plate (601). Two guide rods (603) are slidably connected to the sliding plate (601), and the two guide rods (603) are fixedly connected to the upper side inside the sliding shell (402). A spring is sleeved on the outside of each guide rod (603) in front of the sliding plate (601). A locking plug post (602) passing through the rear side wall of the sliding shell (402) is fixedly connected to the rear end face of the sliding plate (601). The front end face of the sliding plate (601) is fixedly connected to the upper end of the downward pull rope (503).

8. A tumor cell culture device with precisely controllable temperature according to claim 7, characterized in that: The foot-operated mechanism (5) includes a U-shaped support plate (501). The U-shaped support plate (501) is fixedly connected to the front left part of the chassis (106), and four vertical guide rods are arranged inside the U-shaped support plate (501). A foot-operated block (502) is slidably connected inside the U-shaped support plate (501) through the four vertical guide rods. The upper end face of the foot-operated block (502) is fixedly connected to a downward pull rope (503) passing through the top of the U-shaped support plate (501). The downward pull rope (503) also passes through the bottom of the sliding shell (402). The upper end of the downward pull rope (503) is fixedly connected to the front end face of the sliding plate (601), and the downward pull rope (503) is slidably connected to the two pulleys (406).

9. A tumor cell culture device with precisely controllable temperature according to claim 7, characterized in that: When the locking mechanism (6) is in the locking and limiting state, the rear end of the locking plug post (602) is plugged into the limiting jack (405).

10. A tumor cell culture device with precisely controllable temperature according to claim 8, characterized in that: When the foot-operated block (502) is stepped down to the middle position inside the U-shaped support plate (501), the locking plug post (602) will be pulled out from the inside of the limiting jack (405), and at this time, the front end face of the sliding plate (601) contacts the rear end face of the limiting block (407).

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