P.megatherium and application thereof

Abstract

The invention belongs to the technical field of microbial treatment, and particularly relates to a P.megatherium and application thereof.The P.megatherium has the function of efficiently dissolving out high-valence metal cations such as Ca < 2 + > from coal gangue, the functional strain is used for treating coastal solonchak, dissolved-out free-valence metal ions can accurately target Na < + > adsorbed by soil colloid, and the soil colloid adsorption capacity is improved. Na < + > is desorbed from the saline soil body and discharged out of the plough layer soil by virtue of the ion exchange effect, so that the problems of high salinity and single component of the coastal saline soil can be effectively solved, and an advanced and reliable technical means is provided for ecological restoration and agricultural utilization of the coastal saline soil.

Description

Technical Field

[0001] The present invention belongs to the technical field of microbial treatment, and specifically relates to a Priestia megaterium and its application, particularly to a functional bacterium capable of highly dissolving divalent metal cations such as Ca from coal gangue, and based on the ion exchange of soil colloid adsorption, thereby realizing the efficient treatment of coastal saline soil. 2+ and other divalent metal cations, and based on the ion exchange of soil colloid adsorption, thereby realizing the efficient treatment of coastal saline soil. Background Art

[0002] Coastal saline soil is widely distributed in the eastern coastal provinces of China. According to statistics, the area of coastal saline soil in China can reach more than 6.93 million hectares, accounting for 7% of the total area of saline-alkali land, which severely restricts the ecological protection, agricultural development and economic construction in the coastal areas. Coastal saline soil has the characteristics of heavy surface salt accumulation, mainly single sodium salt in salt composition, and high pH value. Therefore, the traditional improvement methods for saline-alkali soil are not completely applicable. Coal gangue is rich in various valuable metal elements such as Ca, and has great potential in adjusting soil pH, replacing sodium ions, and reducing salt damage. At the same time, as a potential kinetic energy for treating coastal saline soil, the stockpile and annual growth of coal gangue are huge. According to official data, in 2024, the production of coal gangue in China reached 825 million tons, a year-on-year increase of 2.1%, and there are still 200 million tons of new coal gangue stockpiled every year, and the cumulative stockpile has exceeded 7 billion tons. If the coal gangue resources can be reasonably utilized, it is expected to build it into a high-value resource for treating coastal saline soil, and then promote the treatment of coastal saline soil and the high-efficiency value-added utilization of coal gangue in a coordinated manner, and synchronously maximize the benefits.

[0003] The main factor restricting the application of coal gangue in the treatment of coastal saline soil is that divalent metal elements such as Ca in it are difficult to be efficiently utilized. Calcium in coal gangue may exist in various forms such as calcium carbonate and calcium silicate, and calcium ions can be slowly released only under certain acidic conditions. In addition, coastal saline soil itself has the characteristic of high salinity, and a large amount of sodium salts, etc. will undergo ion exchange reactions with calcium in coal gangue, which may re-exchange the calcium ions that have been exchanged to the soil particle surface, making it difficult for calcium to stably exist and play a role in the soil. Soil microorganisms can play a key role in the transformation and utilization of Ca, but at present, there is still no report on microbial strains that can both adapt to the high-salt environment of coastal saline soil and efficiently enhance the dissolution of Ca in coal gangue. Summary of the Invention

[0004] The purpose of the present invention is to provide a Priestia megaterium and its application for the problem of low dissolution ability of divalent metal elements in the application of coal gangue in the treatment of coastal saline soil. This strain has the function of efficiently dissolving divalent metal cations such as Ca from coal gangue 2+ and other divalent metal cations, and based on the ion exchange of soil colloid adsorption, realizing the efficient treatment of coastal saline soil.

[0005] To achieve the above object, the present invention adopts the following technical solutions: In the first aspect of the present invention, there is provided a Priestia megaterium, characterized in that the strain is classified and named as Priestia megaterium H16, which was deposited at the China Center for Type Culture Collection on December 12, 2024, with the deposit number CCTCC NO: M 20242801.

[0006] The present invention uses a functional selective medium that only retains the basic carbon source, nitrogen source and buffer system, and replaces the trace element components with coal gangue as the enrichment condition. Samples are taken from the rhizosphere soil of an artificially designed culture and domestication system. Through multi-stage enrichment and separation and purification, 1 strain of salt-tolerant functional microbial strain with the ability to efficiently dissolve high-valent metals such as Ca 2+ from coal gangue (Priestia megaterium 16, Priestia megaterium H16 ) is screened.

[0007] Priestia megaterium H16 is an aerobic bacterium and Gram-negative. On the common beef extract-peptone agar medium, it grows to form light white, smooth, round, convex colonies with complete edges. Priestia megaterium H16 shows good growth ability when the pH value is 4-9. The exogenous addition of 1% (w / v) NaCl can promote its growth. When the NaCl growth tolerance range is ≤ 8% (w / v), OD600 / 9 hours can reach 0.18-0.35.

[0008] This strain has the function of efficiently dissolving high-valent metal cations such as Ca 2+ from coal gangue. This functional strain is used to treat coastal saline soil. The dissolved exchangeable metal ions can accurately target the Na adsorbed by soil colloids + , and through ion exchange, Na + is desorbed from the saline soil colloid and discharged from the plough layer soil, which can effectively improve the problems of high salt content and single composition of coastal saline soil, and provide advanced and reliable technical means for the ecological restoration and agricultural utilization of coastal saline soil.

[0009] In the second aspect of the present invention, there is provided a microbial inoculant containing the above-mentioned Priestia megaterium.

[0010] Furthermore, it includes strains, bacterial solutions or fermentation broths.

[0011] In the third aspect of the present invention, there is provided the application of the above-mentioned microbial inoculant in dissolving high-valent metal ions in coal gangue.

[0012] Furthermore, the high-valent metal ions include calcium ions.

[0013] Applying the functional microbial strain of the present invention to treat coal gangue slag in a liquid culture medium environment, Ca 2+ The initial concentration is 26.37 ± 1.11 μg·mL -1 , after inoculating the strain of Priestia megaterium H16 ( Priestia megaterium H1 6), the concentration of Ca 2+ ions increased rapidly. At the 9th hour after inoculation, the concentration in the system reached 60.32 ± 2.64 μg·mL -1 , and reached the peak at the 24th hour, reaching 70.11 ± 3.71 μg·mL -1 , and the dissolution efficiency increased by 130.58 times compared with that without inoculating the strain.

[0014] The fourth aspect of the present invention provides the application of the above microbial inoculant in treating coastal saline soil.

[0015] Furthermore, treating coastal saline soil includes promoting the discharge of salts in coastal saline soil.

[0016] Furthermore, treating coastal saline soil includes reducing the total salinity of coastal saline soil.

[0017] Applying the functional microorganisms of the present invention to strengthen coal gangue to improve coastal saline soil, adding coal gangue to coastal saline soil at a ratio of 10% (w / w), and spraying the bacterial solution of Priestia megaterium H16 ( -1 6) at a ratio of 5 mL·kg Priestia megaterium H1 , and simulating natural precipitation for leaching. The original total salt content of the plough layer (0 - 20 cm depth) soil is 4.27 ± 0.10 g·kg -1 , after treatment with this technology, it can be reduced to 2.03 ± 0.06 g·kg -1 ; the original sodium ion content is 812.85 ± 25.78 mg·kg -1 , after treatment with this technology, it can be reduced to 277.34 ± 15.07 g·kg -1 ; the Ca / Na ratio increases from 0.60 ± 0.08 to 0.91 ± 0.07. The principle of the present invention is that the bio-enhanced dissolution of high-valent metals in coal gangue leads to the replacement of Na 2+ by Ca + and other substances at the soil colloid interface, thereby further promoting the discharge of salts and the reduction of total salinity. At the same time, the significant increase in the Ca / Na ratio in the soil can not only promote the improvement of coastal saline soil but also promote its further utilization.

[0018] The fifth aspect of the present invention provides a method for dissolving high-valent metal ions in coal gangue, which is treated with the above microbial inoculant.

[0019] In the sixth aspect of the present invention, a method for treating high salt content in coastal saline soil is provided. Coal gangue and the microbial inoculant as described above are added to the coastal saline soil for soil treatment.

[0020] Compared with the prior art, the beneficial effects of the present invention are as follows: 1. The present invention relates to a Priestia megaterium and its application. This strain has the function of efficiently dissolving divalent metal cations such as Ca from coal gangue. This functional strain is used to treat coastal saline soil, and the dissolved divalent metal ions can precisely target the Na adsorbed by soil colloids. 2+ Through ion exchange, Na is desorbed from the salt soil colloid and discharged from the plough layer soil, which can effectively improve the problems of high salt content and single composition in coastal saline soil, and provide advanced and reliable technical means for the ecological restoration and agricultural utilization of coastal saline soil. + +

[0021] 2. In the present invention, microorganisms produce organic acids through their own metabolic activities, which react chemically with calcium carbonate, calcium silicate, etc. in coal gangue to release water-soluble and exchangeable Ca, 2+ and form ion exchange with Na+ in coastal saline soil, so that Na salts are discharged from the plough layer soil, realizing the efficient treatment of coastal saline soil.

[0022] 3. In the present invention, the resource utilization of coal gangue is realized, the discharge of waste is reduced, the resource utilization rate is improved, and significant economic and environmental benefits are achieved. At the same time, the microbial enhanced dissolution technology can avoid the risk of secondary pollution to soil and groundwater; the functional microorganisms have the characteristics of strong colonization ability, fast metabolic rate, and good in-situ action effect, etc., and can continuously play a role, continuously dissolve calcium in coal gangue and improve coastal saline soil, realizing the long-term green treatment of coastal saline soil.

[0023] 4. In the present invention, the microbial enhanced dissolution technology does not require complex pretreatment and processing of coal gangue. Only by adding an appropriate amount of microbial inoculant and creating a suitable microbial growth environment, the dissolution of calcium and the treatment of salt soil can be realized, reducing the cost. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 It is the colony appearance and phylogenetic tree diagram tested in Example 1. Figure 1 a in it is the colony appearance diagram, Figure 1 b in it is the Gram staining diagram, Figure 1 c in it is the phylogenetic tree diagram.

[0025] Figure 2 It is the data graph of the time change of the free Ca ion concentration in the coal gangue leachate in Example 2. 2+ ​​​

[0026] Figure 3 It is a real - shot and schematic diagram of the verification device for microbial - enhanced coal gangue to improve coastal saline soil in Example 3.

[0027] Figure 4 It is a data graph for evaluating the effect of microbial - enhanced coal gangue on improving coastal saline soil. Specific implementation manners

[0028] The present invention will be further described in detail below with reference to specific embodiments. However, it should not be understood that the scope of the above - mentioned subject matter of the present invention is limited to the following embodiments. All technologies implemented based on the content of the present invention belong to the scope of the present invention.

[0029] Example 1 Establishment of a functional selection medium: The formula of the functional selection medium for screening microbial strains with the function of dissolving high - valence metals from coal gangue is as follows: Glucose 10.0 g·L -1 , peptone 5.0 g·L -1 , coal gangue (200 mesh) 2.0 g·L -1 , NaCl 8.0 g·L -1 , KCl 0.2 g·L -1 , Na2HPO4 g·L -1 , KH2PO4 g·L -1 . Put the functional selection medium into a high - temperature steam sterilizer, set the temperature at 121 °C for 30 minutes, cool it to room temperature, and then adjust the pH value to 7.0 with NaOH and HCl solutions for standby.

[0030] Establishment of an artificial culture and domestication system: Mix coastal saline soil (collected from Gudao Town, Dongying City, Shandong Province, 118.42'58'' east longitude, 37.48'59'' north latitude. The soil sampling depth is 0 - 30 cm, removing stones, plant and animal residues, etc. Its properties are shown in Table 1), coal gangue (collected from a certain gangue yard of Shengquan Coal Mine, Tai'an City, Shandong Province, 35°58′ north latitude, 117°28′ east longitude, the sample collection depth is 0 - 30 cm, and its element distribution is shown in Table 2) and commercially available nutrient substrate soil in a ratio of 1:1:1 and plant wheat. On the 90th day of planting, take out the flowerpots and collect rhizosphere soil.

[0031] Table 1 Basic properties of the tested coastal saline soil

[0032] Table 2 Basic properties of the tested coal gangue

[0033] Screening and purification of functional microorganisms: Enrichment culture: After collecting rhizosphere soil, 5.0 g of soil was placed into a conical flask (250 mL) containing 100 mL of functional selection medium, and the temperature was set at 30 °C with a rotation speed of 120 r·min -1 , and it was cultured in an oscillating incubator for 12 hours; b. Plate culture and purification: The bacterial solution was diluted serially by a factor of 10 -1 -10 -9 using diethyl pyrocarbonate-treated water (DEPC water). 100 μL of the diluted solution was inoculated into a petri dish, evenly spread with an L-shaped spreader, and then cultured at 37 °C for 3 days. After the culture was completed, a single colony was picked with a 1 μL inoculation loop and further isolated and purified multiple times on a nutrient agar culture plate. After each purification, it was cultured at 37 °C for 3 days until single colonies were visible to the naked eye on the culture plate; c. Strain preservation: A single colony was picked and inoculated into 5 mL of Luria-Bertani medium (Solarbio, catalog number L1010) with a 1 μL inoculation loop, and then cultured at 37 °C and 120 r·min -1 for 12 hours. After the culture was completed, 800 μL of the culture solution was added to a 2 mL sterile cryopreservation tube, and then 200 μL of sterilized glycerol (80% v / v) was added and mixed well, and it was stored in a -80 °C refrigerator. At the same time, the single colonies on the plate were stored in a 4 °C refrigerator.

[0034] d. Strain identification: An appropriate amount of the bacterial cells was taken and added to a 2 mL centrifuge tube. 567 μL of TE buffer was added, and it was repeatedly pipetted to resuspend. Then 30 μL of 10% SDS and 15 μL of proteinase K were added and mixed well. It was incubated at 55 °C for 30 min, and an equal volume of chloroform:isoamyl alcohol solution with a volume ratio of 24:1 was added, and centrifuged at 12000 r / min for 4 - 5 min. Subsequently, the supernatant was transferred to a new tube, 0.6 - 0.8 times the volume of isopropanol was added, and gently mixed until the DNA precipitated, and centrifuged at 12000 r / min for 10 min. 1 mL of 70% ethanol was added for washing, centrifuged at 12000 r / min for 10 min, and the ethanol was discarded. It was dried in a clean workbench and redissolved in 50 μL of TE buffer or deionized water.

[0035] The sequenced strain sequences were identified by SeqMan splicing, vector sequence removal, and chimera detection, and compared and classified at the National Center for Biotechnology Information (NCBI) in the United States. A total of 1 strain of bacteria, Priestia megaterium H16 ( Priestia megaterium H1 6) belongs to PriestiaGenus, facultative anaerobe, Gram-negative, grows to form white, round, smooth and moist colonies on ordinary beef extract-peptone agar medium, specifically as Figure 1 shown. Figure 1 In which, a is the appearance diagram of the colony, Figure 1 b in which is the Gram staining diagram, Figure 1 c in which is the phylogenetic tree diagram.

[0036] Priestia megaterium H16 ( Priestia megaterium H1 6) has been deposited in the China Center for Type Culture Collection (CCTCC), the deposit address is Wuhan University, No. 299, Bayi Road, Wuchang District, Wuhan City, Hubei Province, and the deposit number is CCTCC M 20242801, and the deposit date is December 12, 2024.

[0037] In the laboratory, the long-term preservation method of the strain is to add the strain to the MB 2216 medium (Difco, catalog number 279110) containing 30% glycerol and store it at -80 °C; the short-term preservation method is to inoculate the strain on the slant of beef extract peptone agar medium and store it at 4 °C for standby.

[0038]

[0039] Example 2 In Example 2, an experiment on promoting the dissolution of high-valent metal ions in coal gangue by strains was carried out, which is specifically as follows: Establishment of a solid waste high-valent metal ion dissolution culture medium: The functional verification culture medium for verifying the efficacy of microorganisms such as strains for dissolving high-valent metal ions such as Ca from coal gangue, and the specific formula is: glucose 10.0 g·L 2+ 、peptone 5.0 g·L -1 、coal gangue powder (100 mesh) 2.0 g·L -1 、NaCl 8.0 g·L -1 、KCl 0.2 g·L -1 、Na2HPO4g·L -1 、KH2PO4g·L -1 -1 。Put the functional selection culture medium into a high-temperature steam sterilizer, set the temperature at 121 °C for 30 minutes, cool to room temperature, and then adjust the pH value to 7.0 with NaOH and HCl solutions for standby.

[0040] Establishment of a functional verification experimental system: This implementation case is divided into an experimental group and a control group, with a total of 2 groups.

[0041] The experimental group was to inoculate the glycerol cryopreservation solution (Example 1) of Priestia megaterium H16 ( Priestia megaterium H1 6) into the above functional verification culture medium at a ratio of 1% (v / v) (250 mL Erlenmeyer flask, containing 100 mL of culture medium liquid), set 3 replicates, and culture at 30 °C and 120 r·min -1 for 7 days to obtain a coal gangue leachate.

[0042] The control group was the functional verification culture medium without inoculating functional microorganisms.

[0043] Determination of dissolution efficiency (concentration of high-valent metal ions): The strengthening effect of functional strains on the dissolution of high-valent metal ions in coal gangue was expressed by the concentration of free Ca 2+ ions in the functional verification culture medium (coal gangue leachate). At 9 hours, 1, 3, 5, and 7 days of culture respectively, 10 mL of liquid was collected, and at 4 °C and 8000 r·min -1 ​Centrifuge for 10 min, take the supernatant, and successively pass it through a 0.45 μm water-based polyethersulfone filter membrane and a C18 small column to remove suspended particulate impurities and organic matter respectively. Finally, dilute it 5 times with 2.5% HNO3, and determine Ca according to the national environmental protection standard method "Determination of 32 Elements in Water Quality - Inductively Coupled Plasma Emission Spectrometry" (HJ 776-2015). 2+ Concentration.

[0044] Evaluation of dissolution efficiency: Priestia megaterium H16 ( PriestiamegateriumH1 6) showed good leaching efficiency for Ca in coal gangue (as 2+ shown). In the functional verification medium, the initial concentrations of Ca Figure 2 were 26.37 ± 1.11 μg·mL 2+ respectively. There was no significant difference in the control group. After inoculating the functional strain, the concentration of Ca -1 increased rapidly. At the 9th hour after inoculation, it reached 60.32 ± 2.64 μg·mL 2+ , and reached the peak at the 24th hour, reaching 70.11 ± 3.71 μg·mL -1 , and the dissolution efficiency increased by 130.58 times. -1 The dissolution efficiency increased by 130.58 times.

[0045] Example 3 In Example 3, a microbial enhanced coal gangue-improved coastal saline soil experiment was carried out as follows: Soil column verification experiment materials and devices: The tested coastal saline soil samples were collected from Gudao Town, Dongying City, Shandong Province (118.42'58'' E, 37.48'59'' N), and its detailed soil properties are listed in Table 3. Select a soil profile with typical characteristics, collect the soil layer at a depth of 0-30 cm. After the soil samples are fully stirred and air-dried, pass them through a 4 mm sieve and fill them into the soil column.

[0046] For Priestia megaterium H16, first place it in Luria-Bertani medium (Solarbio, product number L1010) for a 10-hour constant temperature culture; after the culture is completed, carry out centrifugation at 3000 r·min -1 at a low temperature of 4 °C for 10 min to obtain cell precipitation; wash the obtained cell precipitation 3 times with phosphate buffer solution (PBS); finally, redissolve it with 0.01M phosphate buffer solution (PBS) and adjust the concentration to 10 8 CFU / mL.

[0047] An acrylic transparent column with an inner diameter of 20 cm and a height of 40 cm was selected as the experimental device. During the construction of the device, a hole with a diameter of 1 cm was accurately reserved at the bottom of each soil column to facilitate the smooth discharge of leachate, ensure that the excess water and soluble substances in the soil system can be discharged in time during the experiment, and maintain the stability of the experimental conditions. A layer of quartz sand with a thickness of 5 cm was laid at the bottom of the soil column as a filter layer. With its good particle structure and pore characteristics, it effectively prevents the soil particles at the bottom of the column from agglomerating and blocking the column outlet due to water flow impact or gravity, ensuring the smoothness of leachate discharge; a layer of nylon mesh was carefully laid on top of the quartz sand to further refine the filtration effect, prevent fine soil particles from entering the drainage hole through the quartz sand layer, and at the same time play a certain role in supporting and dispersing the soil layer above, ensuring that the soil is more evenly distributed in the column.

[0048] According to the actual bulk density value (1.4 g・cm - ³), accurately weigh the coastal saline soil required for a height of 30 cm. According to the design plan, the pre-prepared coal gangue slag and bacterial solution are mixed or sprayed in a specific way to make them evenly distributed in the soil. After sufficient mixing and stirring to ensure that all components are evenly distributed, the soil filling operation in the soil column begins. In order to ensure the uniformity and compactness of the soil filling, a batch filling strategy is adopted. Each soil column is filled a total of 6 times, and the filling height is 5 cm each time. After the filling operation is completed, a 5 cm water storage layer is reserved above the soil column (for details, see Figure 3 ), this aquifer can provide a buffer space for water replenishment in subsequent experiments on the one hand, and on the other hand help simulate the water retention conditions in the upper soil layer under natural conditions, ensuring the compatibility of experimental conditions with the actual soil ecological environment.

[0049] Table 3 Basic properties of the tested coastal saline soil

[0050] This implementation case is divided into experimental group 1, experimental group 2 and control group, a total of 3 groups.

[0051] Experimental group 1 was to add coal gangue to coastal saline soil at a ratio of 10% (w / w) and fill the soil column; Experimental group 2 was to add coal gangue to coastal saline soil at a ratio of 10% (w / w), and at the same time add 5 mL·kg -1 ratio, spraying Priesteria gigantea H16 ( Priestia megaterium H1 6) Add bacterial solution and fill the soil column; The control group was simply filled with the same mass of coastal saline soil as the experimental groups without any other treatment.

[0052] Testing and analysis of the efficiency of microbial enhanced gangue improvement: To simulate the salt leaching caused by natural precipitation, the intermittent leaching method was used. The leaching was started every 12 hours, and 300 mL of leaching water was added each time. The experiment lasted for 21 days. After the experiment, the soil in the soil column was taken out, dried naturally in the shade and fully mixed. The soil exchangeable base ion content (K) was determined according to the "Determination of Cation Exchange Capacity and Exchangeable Base of Neutral Soil" (NY / T 295-1995). + 、Na + , Ca 2+ Mg 2+ ); Refer to "Soil Testing Part 18: Determination of Soil Sulfate Ion Content" (NY / T1121.18-2006) to determine the soil sulfate ion content (SO4 2- ); Refer to "Soil Testing Part 17: Determination of Soil Chloride Content" (NY / T 1121.17-2006) to determine the chloride ion content (Cl - ) content; refer to "Analysis of Water-soluble Salt in Forest Soil" (LY / T 1251-1999), determine the carbonate (CO3 2- ) and bicarbonate (HCO3 - ) ion content.

[0053] Evaluation of the efficiency of microbial enhanced coal gangue improvement: Only the addition of coal gangue blasting and simulated precipitation leaching in the soil column of coastal saline soil reduced the total salt content and improved the composition of salt ions to a certain extent, while the inoculation of functional microorganisms significantly enhanced this process, such as Figure 4 As shown. Ca in coal gangue 2+ In situ bioaugmented dissolution leads to the formation of Ca 2+ To Na + At the same time, the significant increase in the Ca / Na ratio in the soil can not only promote the improvement of coastal saline soil, but also promote its further utilization.

[0054] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A Priestia megaterium, characterized in that, The strain is classified and named as Priestia megaterium H16, which was deposited at the China Center for Type Culture Collection on December 12, 2024, with the deposit number of CCTCC NO: M 20242801.

2. A microbial inoculant containing the Priestia megaterium as described in claim 1.

3. The microbial inoculant according to claim 2, characterized in that, It includes the strain, the bacterial liquid or the fermentation broth.

4. The application of the microbial inoculant as described in claim 2 in dissolving high-valent metal ions in coal gangue.

5. The application according to claim 4, wherein The high-valent metal ions include calcium ions.

6. The application of the microbial inoculant as described in claim 2 in treating coastal saline soil.

7. The application according to claim 6, characterized in that, Treating coastal saline soil includes promoting the discharge of salts in coastal saline soil.

8. The application according to claim 6, characterized in that, Treating coastal saline soil includes reducing the total salinity of coastal saline soil.

9. A method for dissolving high-valent metal ions in coal gangue, characterized in that, Treatment is carried out using the microbial inoculant as described in claim 2.

10. A method for treating high salinity in coastal saline soil, characterized in that, Coal gangue and the microbial inoculant as described in claim 2 are added to the coastal saline soil for soil treatment.

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