Illumination-regulated water and gas CO2 synchronous measurement device and use method thereof

Abstract

The invention provides an illumination-controlled water and gas CO2 synchronous measurement device and a use method, the illumination-controlled water and gas CO2 synchronous measurement device comprises an experiment support, an experiment container, an illumination assembly, a gas guide pipe assembly, a water guide pipe assembly and a monitor, the experiment container is placed in the experiment support, the illumination assembly is arranged above the experiment container, a sealing cover is arranged on the experiment container, and the illumination assembly is arranged on the experiment support. The shading film component is arranged on the sealing cover and consists of various shading films with different light transmittances; the upper end part of the experimental container is connected with a monitor through an air guide pipe assembly, the monitor is configured to measure the CO2 content of air at the upper end part of the experimental container, and the lower end part of the water guide pipe assembly is immersed in a solution at the lower end part of the experimental container and is used for collecting a water sample in the experimental container; the CO2 concentration change condition in air and water can be monitored at the same time, different light and dark periods can be simulated by arranging the LED lamp controller and the shading object, the device is more suitable for the field environment, and the applicability and reliability of the result of the measuring device are improved.

Description

Technical Field

[0001] The present invention relates to the technical field of environmental monitoring, in particular to a device for synchronously measuring water and air CO2 with light regulation and its usage method. Background Art

[0002] Marine carbon sink (also known as "blue carbon") refers to the process and mechanism of absorbing carbon dioxide in the atmosphere by taking the ocean as a specific carrier and solidifying it. At present, there has always been a controversy over whether shellfish in seawater are carbon sinks or carbon sources. Accurately estimating the CO2 gas exchange flux at the air-sea interface is an important indicator for evaluating the characteristics of ocean CO2 sources / sinks. The research on CO2 exchange at the air-sea interface in shellfish and seaweed cultivation areas mainly relies on the method of the CO2 partial pressure difference at the air-sea interface. The data acquisition methods for pCO2 in water bodies mainly include two categories: closed systems and cruise observations. However, these methods all have certain limitations and cannot comprehensively reflect the dynamic behaviors of aquatic organisms in natural environments. For example, existing technologies usually can only measure the CO2 concentration in water or air alone, and cannot synchronously obtain the dynamic change data of both. In addition, existing experimental devices often cannot simulate real field environments, such as changes in light intensity and light-dark cycles, which limits the reliability and applicability of experimental results and brings many troubles to the assessment of the carbon sink contribution of marine ecosystems. Summary of the Invention

[0003] Aiming at the deficiencies existing in the prior art, the purpose of the present invention is to provide a device for synchronously measuring water and air CO2 with light regulation and its usage method, which can effectively solve the problems that existing experimental devices cannot accurately monitor the data of the fixation, storage, and release of CO2 by different organisms, and cannot objectively evaluate the carbon sink effect or carbon source effect of different organisms.

[0004] To achieve the above purpose, the present invention is realized through the following technical solutions:

[0005] The present invention provides a device for synchronously measuring water and air CO2 with light regulation, including: an experimental support, an experimental container, a lighting component, a gas pipe component, a water pipe component, and a monitor, characterized in that;

[0006] The experimental container is placed inside the experimental support, the lighting component is arranged above the experimental container, the experimental container is equipped with a sealing cover, and a light-shielding film component configured on the sealing cover, and the light-shielding film component is composed of light-shielding films with various light transmittances; the upper end of the experimental container is connected to the monitor through the gas pipe component, and the monitor is configured to measure the CO2 content of the air at the upper end of the experimental container, and the lower end of the water pipe component is immersed in the solution at the lower end of the experimental container for collecting the water sample inside the experimental container.

[0007] As a further improvement, the experimental support includes: a first support and a second support, and the second support is slidably sleeved on the first support.

[0008] As a further improvement, a plurality of positioning holes are provided on the side wall of the first support, and a clamping block is provided on the second support on the side close to the positioning holes.

[0009] As a further improvement, a mounting plate is connected to the side of the second support away from the first support, and a lamp slot for placing the lighting component is provided on the mounting plate.

[0010] As a further improvement, the experimental container includes: a sealing cover and a tank body, a sealing ring is provided on the sealing cover, and sealing rings are provided at the joints of the sealing cover with the air duct assembly and the water duct assembly.

[0011] As a further improvement, the lighting component includes: an LED lamp and an LED lamp controller, and the LED lamp controller is configured to control the light intensity of the LED lamp.

[0012] As a further improvement, the air duct assembly includes: an air delivery pipe and a conduit, and a quick connector configured on the conduit; the water duct assembly includes: a water sample collection pipe and a stop clamp configured on the water sample collection pipe.

[0013] A usage method of a water and gas CO2 synchronous measurement device with light control includes the following steps:

[0014] S1, adjust the height of the LED lamp, set the light intensity of the high-light group in the range of 20000 lx to 100000 lx; adjust the LED lamp to the lowest gear, set different light-shielding films, and the light intensity of the low-light group is in the range of 1000 lx to 20000 lx; place the measurement device in a dark room to set a dark control group;

[0015] S2, inject seawater to the position of 1 / 3 to 2 / 3 of the height of the tank body, put in the experimental samples, and close the sealing cover to detect the airtightness of the device;

[0016] S3, set different light-dark cycles, set the ratio of the light time to the dark time at 10h:14h, simulate the natural light change in the wild, and perform water sample collection and air C02 concentration before the end of each light-dark cycle; monitor the change of CO2 concentration in water and air;

[0017] S4, when detecting, connect the air duct assembly to the monitor to detect the CO2 concentration in the air, and according to the measured data, through A=(C tg -C og )×V gObtain the diffusion rate of CO2 in water and air per unit area by (A×t), where A is the diffusion rate, C tg is the concentration of CO2 in the air at time t, and C og is the initial concentration of CO2 in the air, V g is the volume of air in the device, S is the water surface area, and t is the experimental time; and a certain amount of water sample is extracted through the water pipe assembly to detect multiple indexes in the water;

[0018] S5. After the sampling is completed, inject air of the same volume into the tank through the air pipe assembly according to the volume of the extracted water sample.

[0019] The beneficial effects of the present invention are as follows:

[0020] By setting up the lighting component, the present invention can simulate different light intensities, and by setting shading films with different light transmittances on the sealing cover, the measuring device can simulate the real light intensity in the wild, making the environment in the experimental container more in line with the wild environment, enabling the object to be detected to carry out normal biological activities, and improving the reliability and effectiveness of the experimental results; by setting sealing rings and gaskets on the sealing cover, the experimental container is in a closed environment to prevent gas leakage in the experimental container from affecting the experimental results. By setting an experimental support with adjustable height, the distance between the lighting component and the experimental container can be adjusted to further control the light intensity irradiated on the experimental sample and simulate a more real wild environment. By connecting the air pipe assembly and the water pipe assembly to the monitor, the CO2 in the air and water in the experimental container can be synchronously monitored, accurately grasping the change of CO2 concentration in the air and water in the experimental container, and then analyzing whether the object to be measured is a carbon source or a carbon sink through the change of CO2 concentration. Description of the Drawings

[0021] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following will briefly introduce the drawings required for the embodiments. It should be understood that the following drawings only show some embodiments of the present invention, so they should not be regarded as limiting the scope. For those of ordinary skill in the art, without creative efforts, other related drawings can also be obtained based on these drawings.

[0022] Figure 1 is a schematic structural diagram of a device and a usage method for synchronously measuring CO2 in water and air with light intensity regulation according to the present invention.

[0023] Figure 2 is a schematic cross-sectional structural diagram of a device and a usage method for synchronously measuring CO2 in water and air with light intensity regulation according to the present invention.

[0024] Figure 3 is a schematic structural diagram of an experimental support of a device and a usage method for synchronously measuring CO2 in water and air with light intensity regulation according to the present invention.

[0025] Figure 4 It is a schematic cross-sectional view of the experimental support structure of a device for synchronously measuring water and gaseous CO2 with light regulation and its usage method according to the present invention.

[0026] Figure 5 It is a schematic structural view of the experimental container of a device for synchronously measuring water and gaseous CO2 with light regulation and its usage method according to the present invention.

[0027] Figure 6 It is a schematic cross-sectional view of the experimental container of a device for synchronously measuring water and gaseous CO2 with light regulation and its usage method according to the present invention.

[0028] In the figure: 1 - experimental support, 11 - first support, 111 - positioning hole, 12 - second support, 121 - clamping block, 13 - mounting plate, 131 - lamp slot, 2 - experimental container, 21 - sealing cover, 211 - quick connector, 212 - sealing ring, 213 - sealing ring, 22 - tank body, 3 - lighting assembly, 4 - gas transmission pipe, 5 - water sample collection pipe. Specific embodiments

[0029] To make the purposes, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are some, but not all, of the embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts fall within the protection scope of the present invention. Therefore, the following detailed description of the embodiments of the present invention provided in the drawings is not intended to limit the scope of the claimed present invention, but merely represents selected embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

[0030] In the description of the present invention, the terms "first" and "second" are only used for descriptive purposes and cannot be construed as indicating or implying relative importance or implicitly specifying the quantity of the indicated technical features. Thus, the features defined with "first" and "second" may explicitly or implicitly include one or more of such features. In the description of the present invention, "a plurality" means two or more unless otherwise specifically defined.

[0031] Refer to Figure 1-6 As shown, a device for synchronously measuring water and gaseous CO2 with light regulation and its usage method includes: an experimental support 1, an experimental container 2, a lighting assembly 3, a gas conduit assembly, a water conduit assembly, and a monitor;

[0032] The experimental container 2 is placed inside the experimental support 1, the lighting component 3 is configured above the experimental container 2, a sealing cover 21 is configured on the experimental container 2, and a light-shielding film component is configured on the sealing cover 21, and the light-shielding film component is composed of light-shielding films with various light transmittances; the upper end of the experimental container 2 is connected to the monitor through an air duct component, and the monitor is configured to measure the CO2 content of the air at the upper end of the experimental container 2, and the lower end of the water duct component is immersed in the solution at the lower end of the experimental container 2 for collecting the water sample inside the experimental container 2; by arranging the air duct component and the water duct component on the experimental container 2, the synchronous monitoring of CO2 in the air and water in the experimental container is realized, and the change of the CO2 concentration in the air and water in the experimental container is accurately grasped, and then whether the tested shellfish or seaweed is a carbon sink or a carbon source is inferred by analyzing the change of the CO2 concentration.

[0033] Furthermore, in order to improve the accuracy of CO2 concentration monitoring and the reliability of experimental results, the experimental container 2 includes: a sealing cover 21 and a tank body 22. A sealing ring 213 is arranged on the sealing cover 21, and the sealing cover 21 is arranged on the tank body 22 through the sealing ring 213. By frictionally contacting the inner wall of the tank body 22 through the sealing ring 213, the sealing cover 21 is stably placed on the tank body 22, and the leakage of gas from the gap between the sealing cover 21 and the tank body 22 is blocked through the sealing ring 213. Several air duct components and water duct components are penetrated through the sealing cover 21. The air duct component extends into the upper end of the tank body 22 for extracting the gas at the upper end, and the water duct component extends into the lower end of the tank body 22 for extracting the water sample in the tank body 22. A light-shielding film component is also arranged on the sealing cover 21. A single layer or multiple layers of light-shielding films can be arranged inside the light-shielding film component, and different light intensities are simulated in cooperation with the LED lamp to meet different experimental conditions. To simulate the wild dark environment, a light-shielding object can be coated on the outer wall of the tank body 22. In this embodiment, the light-shielding object is tinfoil paper.

[0034] The air duct component includes: an air delivery pipe 4 and a conduit. A quick connector 211 is arranged on the conduit. The quick connector 211 is used for quickly connecting the monitor through a pipeline, and extracts the gas at the upper end of the experimental container 2 during connection, so that the gas is transported to the monitor through the pipeline. After the sampling is completed, the tank body 22 is sealed after disconnecting from the conduit to prevent internal gas leakage; a stop clamp is arranged on the air delivery pipe 4. When introducing gas into the tank body 22, the stop clamp is opened to connect the air delivery pipe 4 to the gas delivery device, and the gas is introduced into the tank body 22 through the air delivery pipe 4.

[0035] The water conduit assembly includes: a water sample collection tube 5 and a stop clamp. The stop clamp is provided on the water sample collection tube 5. When collecting water samples, the water sample collection tube 5 is connected to the monitor. The stop clamp is opened to make the pipeline of the water sample collection tube 5 unobstructed. The liquid in the experimental container 2 flows along the water sample collection tube 5 to the monitor. After sampling, the stop clamp is closed to tightly seal the pipeline of the water sample collection tube 5 at the stop clamp; the sealing cover 21 is provided with a sealing ring 212 around the connection with the gas conduit assembly and the water conduit assembly to prevent gas from leaking through the gap between the sealing cover 21 and the gas conduit assembly and the water conduit assembly, which may affect the experimental results and cause misjudgment by the experimenter.

[0036] Further, the experimental container 2 is placed in the experimental bracket 1. The experimental bracket 1 includes: a first bracket 11, a second bracket 12, and a mounting plate 13. A number of the first brackets 11 are provided on the base. The second bracket 12 is sleeved inside the first bracket 11. The first bracket 11 is provided with a number of positioning holes 111 on the side wall. The second bracket 12 is provided with a clamping block 121 on the side close to the positioning holes 111. Through the cooperation between the positioning holes 111 and the clamping block 121, the connection position between the first bracket 11 and the second bracket 12 is adjusted, and then the height of the experimental bracket 1 is adjusted. The second bracket 12 is further provided with a mounting plate 13 on the side away from the first bracket 11. The mounting plate 13 is provided with a lamp slot 131 for placing the lighting assembly 3.

[0037] Further, in order to simulate the natural light in the wild, a lighting assembly 3 is provided at the mounting plate 13. The lighting assembly 3 includes: an LED lamp and an LED lamp controller. The LED lamp controller is used to control the light intensity of the LED lamp. The LED lamp controller is configured with adjustment gears, and the adjustment gears can control the light intensity emitted by the LED lamp. If the experimental object is a high-light group, the required light intensity for the high-light group is above 20000 lx, then the corresponding light intensity is set through the adjustment gears. In this embodiment, the light intensity range of the high-light group is 20000 lx to 100000 lx; if the experimental object is a low-light group, the required light intensity for the low-light group is below 20000 lx, then the adjustment gears need to be set to the lowest gear, and the required light intensity is obtained by cooperating with the light-shielding film assembly provided on the sealing cover 21. In this embodiment, the light intensity range of the low-light group is 1000 lx to 20000 lx; the light intensity adjustment of the LED lamp is used to simulate the lighting environment most suitable for the experimental object, and by setting different light-dark cycles, different external environments are simulated to observe the changes in CO2 under different environments, so as to better obtain the experimental results.

[0038] Further, the CO2 concentration in the air is detected through the air duct assembly, and a certain amount of water sample is aspirated through the water duct assembly. In this embodiment, the aspirated water sample is 300 - 500 ml, and indicators such as water temperature, salinity, pH, dissolved oxygen (DO), dissolved inorganic nitrogen (DIN), dissolved inorganic phosphorus (DIP), dissolved inorganic carbon (DIC), and dissolved organic carbon (DOC) in the water sample are monitored.

[0039] Based on the change curve of the CO2 concentration in the air, the carbon source effect or carbon sink effect of the experimental organisms is judged. If the CO2 concentration rises during the experiment, it is a carbon source effect; if the CO2 concentration drops, it is a carbon sink effect. The calculation formula for the CO2 diffusion rate between water and air per unit area: A = (C tg - C 0g ) × V g / (S × t), where A is the diffusion rate, C tg is the CO2 concentration in the air at time t, C 0g is the initial CO2 concentration in the air, V g is the air volume, S is the water surface area, and t is the experimental time. If the value of A is positive, it is a carbon source; if it is negative, it is a carbon sink.

[0040] Based on the increase in the CO2 amount in the air, the rate at which shellfish emit CO2 into the air is calculated: B = (C tg - C 0g ) × V g / (m × t), where B is the emission rate, C tg is the CO2 concentration in the air at time t, C 0g is the initial CO2 concentration in the air, V g is the air volume, m is the weight of the oyster, and t is the experimental time.

[0041] Based on the decrease in the CO2 amount in the air, the rate at which macroalgae absorb CO2 from the air is calculated: B = (C 0g - C tg ) × V g / (m × t), where B is the absorption rate, C 0g is the initial CO2 concentration in the air, C tg is the CO2 concentration in the air at time t, V g is the air volume, m is the weight of the macroalgae, and t is the experimental time.

[0042] Based on the sum of the DIC in the water and the decrease in the CO2 amount in the air during the light cycle, the photosynthetic carbon fixation rate of the algae is calculated: C = ((C 0g - C tg ) × V g + (C 0w - C tw ) × V w ) / (m1 × t), where C is the carbon fixation rate, C0g is the initial concentration of CO2 in the air, C tg is the concentration of CO2 in the air at time t, V g is the volume of air, C 0w is the initial concentration of DIC in water, C tw is the concentration of DIC in water at time t, V w is the volume of water body, m1 is the weight of macroalgae, and t is the experimental time.

[0043] Calculate the CO2 release rate of shellfish respiration and calcification through the sum of the increase in DIC in water and CO2 in the air: D = ((C tg - C 0g ) × V g + (C tw - C 0w ) × V w ) / (m2 × t), where D is the CO2 release rate, C tg is the concentration of CO2 in the air at time t, C 0g is the initial concentration of CO2 in the air, V g is the volume of air, C tw is the concentration of DIC in water at time t, C 0w is the initial concentration of DIC in water, V w is the volume of water body, m2 is the weight of shellfish, and t is the experimental time.

[0044] Calculate the DOC release rate of macroalgae or shellfish through the increase in DOC in water: E = (C tw - C 0w ) × V w / (m × t), where E is the DOC release rate, C tw is the concentration of DOC in water at time t, C 0w is the initial concentration of DOC in water, V w is the volume of water body, m is the weight of macroalgae or shellfish, and t is the experimental time.

[0045] Calculate the carbon storage rate of macroalgae through the increased carbon amount in the macroalgae thallus: F = (m t - m0) × (1 - f) × k / (m0 × t), where F is the carbon storage rate of macroalgae, m t is the fresh weight at the end, m0 is the fresh weight at the start of the experiment, f is the moisture content of the thallus, k is the carbon content (dry weight) of the thallus, and t is the experimental time.

[0046] Determine the influence effect of the macroalgae biological pump on the solubility pump by analyzing the change in the concentration of CO2 in the air caused by macroalgae at a certain light intensity and pH value; by calculating Ca in water 2+Analyze the effects of the macroalgae biological pump on the carbonate pump by varying the concentration and CaCO3 precipitation, and establish a multi-pump coupling mechanism.

[0047] A method for using a light-regulated device for synchronous measurement of water and gas CO2, comprising the following steps:

[0048] S1, Adjust the height and gear of the LED lamp to set the light intensity of the high-light group between 20000 lx and 100000 lx; adjust the LED lamp to the lowest gear, set different light-shielding films, and the light intensity of the low-light group is between 1000 lx and 20000 lx; place the measuring device in a dark room to set a dark control group;

[0049] S2, Inject seawater to the position of 1 / 3 - 2 / 3 of the height of the tank body 22, place the experimental samples, and close the sealing cover 21 to detect the airtightness of the device;

[0050] S3, Set different light-dark cycles, set the ratio of light time to dark time at 10h:14h to simulate the natural light changes in the wild, and collect water samples and monitor the air CO2 concentration before the end of each light-dark cycle; monitor the CO2 concentration changes in water and air;

[0051] S4, When detecting, connect the air duct assembly to the monitor to detect the CO2 concentration in the air, and calculate the diffusion rate of water and gas CO2 per unit area according to the measured data through A=(C tg -C og )×V g / (S×t), where A is the diffusion rate, C tg is the CO2 concentration in the air at time t, C og is the initial CO2 concentration in the air, V g is the air volume in the device, S is the water surface area, and t is the experimental time;

[0052] S5, Extract a certain amount of water samples through the air duct assembly to detect various indicators in the water; when extracting water samples, supplement air with the same volume as the water samples into the tank body 22 through the air duct assembly.

[0053] Example 1:

[0054] Set 30L tanks 22 in the measuring device and have multiple sets. Add 20L of filtered seawater into the tanks 22, and put 10g of Gracilaria lemaneiformis in each tank 22. Also, control the light intensity of the measuring device at 0 lx to 80000 lx. Divide each measuring device into a high-light group and a low-light group. Set the light intensity of the high-light group at 22000 lx to 80000 lx, and adjust the gear positions of the high-light group among 20%, 40%, 60%, 80%, and 100%. Set the light intensity of the low-light group at 0 lx to 20000 lx, and set the gear position of the low-light group at 20%. Set a corresponding dark control group. Conduct sampling and detection before the end of each light-dark cycle. The experimental time is 1 month.

[0055] Example 2:

[0056] Set 30L tanks 22 in the measuring device and have multiple sets. Add 20L of filtered seawater into the tanks 22, and respectively set three polyculture groups of 160g of oysters and 5g of Gracilaria lemaneiformis, 160g of oysters and 10g of Gracilaria lemaneiformis, and 160g of oysters and 20g of Gracilaria lemaneiformis, as well as a single-culture group of 160g of oysters or 20g of Gracilaria lemaneiformis. Set the light intensity at 22000 lx, and set a corresponding dark control group. Conduct sampling and detection before the end of each light-dark cycle. The experimental time is 3 days.

[0057] Example 3:

[0058] Set 30L tanks 22 in the measuring device and have multiple sets. Add 20L of filtered seawater into the tanks 22, and add high-calcium and high-magnesium seawater crystals into each tank 22. Put 10g of Gelidium amansii in it. Select some from several groups of measuring devices and open their sealing covers 21 to make them in an open state, and set the remaining measuring devices in a closed state. Add nitrogen and phosphorus inorganic nutrients into all measuring devices. The initial concentration of DIN is 0.5 g / L, and the concentration of DIP is 0.05 g / L. Set the light intensity at 20000 lx to 80000 lx, and set the gear position at 20%. Conduct sampling and detection before the end of each light-dark cycle. The number of experiments is 3 times. The first time is 3 days, the second time is 1 day, and the third time is 2 days.

[0059] The above is only the preferred embodiment of the present invention and is not used to limit the present invention. For those skilled in the art, the present invention can have various changes and modifications. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A light-regulated synchronous water and gas CO2 measurement device, comprising: Experimental support (1), experimental container (2), lighting component (3), air duct component, water pipe component, monitor Characterized in that; The experimental container (2) is placed inside the experimental support (1), the lighting component (3) is configured above the experimental container (2), the experimental container (2) is equipped with a sealing cover (21), and a light-shielding film component configured on the sealing cover (21), and the light-shielding film component is composed of light-shielding films with various light transmittances; The upper end of the experimental container (2) is connected to the monitor through the air duct component, and the monitor is configured to measure the CO2 content of the air at the upper end of the experimental container (2). The lower end of the water pipe component is immersed in the solution at the lower end of the experimental container (2) for collecting the water sample inside the experimental container (2).

2. The light-regulated water and gas CO2 synchronous measurement device according to claim 1, wherein The experimental support (1) includes: a first support (11) and a second support (12), and the second support (12) is slidably sleeved on the first support (11).

3. The light intensity-regulated water and gas CO2 synchronous measurement device according to claim 2, characterized in that A number of positioning holes (111) are provided on the side wall of the first support (11), and a clamping block (121) is provided on the second support (12) on the side close to the positioning holes (111).

4. The light-regulated water and gas CO2 synchronous measurement device according to claim 2, characterized in that The second support (12) is connected with a mounting plate (13) on the side far from the first support (11), and a lamp slot (131) for placing the lighting component (3) is provided on the mounting plate (13).

5. The light-regulated water and gas CO2 synchronous measurement device according to claim 1, characterized in that The experimental container (2) includes: a sealing cover (21) and a tank body (22), a sealing ring (213) is provided on the sealing cover (21), and sealing rings (212) are provided at the joints of the sealing cover (21) with the air duct component and the water pipe component respectively.

6. The light-regulated water and gas CO2 synchronous measurement device according to claim 1, wherein The lighting component (3) includes: an LED lamp and an LED lamp controller, and the LED lamp controller is configured to control the light intensity of the LED lamp.

7. The light-regulated water and gas CO2 synchronous measurement device according to claim 1, wherein The air duct component includes: an air delivery pipe (4) and a conduit, and a quick connector (211) configured on the conduit; the water pipe component includes: a water sample collection pipe (5) and a stop clamp configured on the water sample collection pipe (5).

8. A method for using the water and gas CO2 synchronous measurement device with light regulation according to any one of claims 1-7, characterized in that, Including the following steps: S1, adjust the height of the LED lamp, set the light intensity of the high-light group at 20000lx - 100000lx; adjust the LED lamp to the lowest gear, set different light-shielding films, and the light intensity of the low-light group is between 1000lx - 20000lx; place the measuring device in a dark room to set a dark control group; S2, inject seawater to the position of 1 / 3 - 2 / 3 of the height of the tank body (22), put in the experimental samples, and close the sealing cover (21) to detect the airtightness of the device; S3, set different light-dark cycles, set the ratio of the lighting time to the dark time at 10h:14h, simulate the natural light change in the wild, and perform water sample collection and air CO2 concentration monitoring before the end of each light-dark cycle; monitor the CO2 concentration changes in the water and air; S4. During detection, connect the air duct assembly to the monitor to detect the CO2 concentration in the air, and calculate the CO2 diffusion rate of water and air per unit area according to the measured data through A = (C tg - C og ) × V g / (S × t), where A is the diffusion rate, C tg is the CO2 concentration in the air at time t, C og is the initial CO2 concentration in the air, V g is the air volume in the device, S is the water surface area, and t is the experimental time; S5, extract a certain amount of water samples through the water pipe component to detect various indicators in the water; when extracting water samples, supplement air with the same volume as the water samples into the tank body (22) through the air duct component.

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