Bacillus amyloliquefaciens and its application

Abstract

The present invention discloses a strain of Bacillus amyloliquefaciens and its application. Bacillus amyloliquefaciens SF-00595, obtained by heat-resistance acclimation of Bacillus amyloliquefaciens SDF-005 and deposited with CCTCC No. M 2025830, can be used as a biopesticide to control papaya anthracnose and fig anthracnose. Heat-resistance acclimation of Bacillus amyloliquefaciens SF-00595 reduces mortality during spray drying of the fermentation broth and increases the viable bacterial content in the powder.

Description

Technical Field

[0001] The present invention belongs to the technical field of microorganisms, and in particular relates to a Bacillus amyloliquefaciens strain and application thereof. Background Art

[0002] Fig( Ficus arica Linn.) is a perennial subtropical deciduous fruit tree, small tree or shrub. Figs have high nutritional value. In addition to nutrients such as sugar, protein, fat and minerals, the fruit also contains trace elements such as copper, iron, zinc, manganese, selenium and rich vitamins. Both the fruit and leaves of figs have certain oxidizing properties. The ethanol extract has a significant broad-spectrum antibacterial effect, and the latex hexane extract has a strong bactericidal effect. Fig latex extract can inhibit the formation and growth of capillaries in umbilical vein endothelial cells. Eating figs can effectively prevent cancer and other chronic diseases, and can also lower blood sugar and blood lipids. It is known as the "guardian of human health in the 21st century." Papaya ( Pseudocydonia sinensis (Thouin) CK Schneid.) is a shrub or small tree in the genus Chaenomeles in the Rosaceae family. Papaya has both economic and medicinal value, primarily for its medicinal uses. Fresh medicinal papaya fruit has attracted considerable attention for its unique nutritional profile.

[0003] Figs and papayas have become emerging economic forestry industries. However, due to climate change, shifts in cultivation practices, and the rapid expansion of cultivated areas, fig and papaya pests and diseases have become increasingly frequent and recurring. Anthracnose, in particular, has become a serious threat to the healthy development of the industry. Currently, fig and papaya pest and disease control primarily relies on chemical pesticides, resulting in increasing pathogen resistance, decreased control effectiveness, and excessive pesticide residues, seriously impacting both fruit quality and ecological safety.

[0004] Microbial-derived biopesticides have become a research hotspot in the field of biopesticides and the main choice for biopesticide registration due to their advantages such as fast reproduction speed, long-lasting control effect, multiple development and utilization channels, low resistance, simple fermentation process and low production cost. Bacillus amyloliquefaciens ) is an effective biocontrol bacterium and a stable biopesticide strain developed in recent years. Bacillus amyloliquefaciens SDF-005 was isolated from rhizospheric soil of a walnut forest in the southern mountainous area of ​​Jinan City, Shandong Province, using the dilution separation method. This strain is deposited under the CGMCC No. 6672. This strain has a strong inhibitory effect against Colletotrichum walnutii and is used to control walnut anthracnose. Based on this, it is necessary to screen for new strains and expand their applications. Summary of the Invention

[0005] To address the above issues, the present invention provides a Bacillus amyloliquefaciens strain and its applications. By acclimating Bacillus amyloliquefaciens SDF-005 (CGMCC No. 6672) to heat resistance, the strain SF-00595 was obtained, which has strong heat resistance and is suitable for controlling papaya anthracnose and fig anthracnose. This strain can be used as a biopesticide to control papaya anthracnose and fig anthracnose, and has promising application prospects.

[0006] The above-mentioned object of the present invention is achieved through the following technical solutions: the Bacillus amyloliquefaciens provided by the present invention ( Bacillus amyloliquefaciens SF-00595 was obtained by acclimating Bacillus amyloliquefaciens SDF-005 (CGMCC No. 6672) to heat resistance (capable of withstanding temperatures of 95°C). It was deposited with the China Center for Type Culture Collection (CCTCC) at Wuhan University, Wuhan, China, on April 21, 2025, under the CCTCC No. M2025830. It exhibits the following microbiological characteristics: rod-shaped, Gram-positive, peritrichous flagella, and facultative anaerobic. Colonies grown on NA medium are round, white, and flat, with a rough surface, a central circular protrusion, and neat edges. Liquid culture produces a biofilm, resulting in a brownish-yellow, turbid, and precipitated liquid. NA medium is used for standard culture, and 20% glycerol is used for long-term storage.

[0007] The present invention also provides a biological bacterial agent with Bacillus amyloliquefaciens SF-00595 as an effective ingredient.

[0008] The preparation method of the above-mentioned biological agent is:

[0009] 1) Shaking culture: Pick a small number of Bacillus amyloliquefaciens SF-00595 colonies from the slant of the test tube, transfer them to the seed medium, and culture them in a shaking incubator at 28-32°C for 12-14 hours.

[0010] 2) Seed liquid culture: Add the shaker-cultured bacterial solution to a seed tank containing seed liquid culture medium and culture at a temperature of 35-40°C, an initial pH of 7.0-7.5, and a culture time of 12-14 hours to obtain seed liquid;

[0011] 3) Fermentation culture: The seed liquid is transferred to the fermentation medium for culture to obtain the fermentation liquid. The culture temperature is 35-40°C, the initial pH is 7.0-7.5, the inoculation amount (mass ratio) is 3-10%, and the culture time is 20-30 hours. The fermentation liquid is then further prepared into a bio-inoculate.

[0012] The seed liquid culture medium is (weight percentage): soybean meal 3.2%, corn starch 2.2%, glucose 0.5%, manganese sulfate 0.02%, and the rest is water, pH 7.0-7.5.

[0013] The fermentation medium is (percentage by weight): soybean meal 5.9%, corn starch 4.8%, glucose 0.5%, manganese sulfate 0.02%, and the rest is water, with a pH of 7.0-7.5.

[0014] Furthermore, the fermentation liquid is added with a water-soluble carrier and spray-dried, the spray-dried bacterial powder is poured into a mixer, and the water-soluble carrier is continuously added and stirred evenly to obtain a biological bacterial agent.

[0015] The present invention also provides use of Bacillus amyloliquefaciens SF-00595 and a biological bacterial agent thereof for controlling (preventing and treating) papaya anthracnose.

[0016] The present invention also provides use of Bacillus amyloliquefaciens SF-00595 and a biological bacterial agent thereof for preventing and treating fig anthracnose.

[0017] The present invention also provides a method for preventing and controlling papaya anthracnose and fig anthracnose, which comprises applying a Bacillus amyloliquefaciens SF-00595 biological agent to papaya and figs with anthracnose.

[0018] The technical effects of the present invention are:

[0019] 1. The Bacillus amyloliquefaciens SF-00595 strain has been acclimated to heat resistance, which reduces the mortality rate of the strain during spray drying of the fermentation broth and increases the content of live bacteria in the bacterial powder.

[0020] 2. Using the spray method, compared with the clean water control, the prevention effect of Bacillus amyloliquefaciens SF-00595 agent 2 treatment (100 and 200 times dilution) on papaya anthracnose and fig anthracnose was more than 70%, and the treatment effect of Bacillus amyloliquefaciens SF-00595 agent 3 treatment (100, 200, and 300 times dilution) was more than 60%, with good prevention and treatment effects.

[0021] 3. Since the Bacillus amyloliquefaciens SF-00595 agent has no drug residue and no toxic side effects, it can reduce the use of chemical pesticides and therefore has good application prospects in the biological control of papaya anthracnose and fig anthracnose. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 This is a single colony morphology of Bacillus amyloliquefaciens SF-00595;

[0023] Figure 2 Figure 3 shows the antibacterial effect of Bacillus amyloliquefaciens SF-00595 on Colletotrichum syringae pv. fig. Figure A shows the antibacterial effect of Bacillus amyloliquefaciens SF-00595 on Colletotrichum syringae pv. fig. Figure B shows the control group inoculated with only Colletotrichum syringae pv. fig.

[0024] Figure 3 Figure 3 is the antibacterial effect of Bacillus amyloliquefaciens SF-00595 against papaya anthracnose, where Figure A shows the antibacterial effect of Bacillus amyloliquefaciens SF-00595 against papaya fruit anthracnose; Figure B is the control group inoculated with only papaya anthracnose. DETAILED DESCRIPTION

[0025] The present invention will be further described below with reference to specific embodiments, and the advantages and features of the present invention will become clearer as the description proceeds. However, these embodiments are merely illustrative and do not constitute any limitation to the scope of the present invention. It should be understood by those skilled in the art that the details and forms of the technical solutions of the present invention may be modified or replaced without departing from the spirit and scope of the present invention, and such modifications and replacements fall within the scope of protection of the present invention.

[0026] Example 1: Isolation and identification of Bacillus amyloliquefaciens SF-00595

[0027] 1. Separation:

[0028] The new strain of Bacillus amyloliquefaciens of the present invention is obtained by acclimating Bacillus amyloliquefaciens SDF-005 (CGMCC No. 6672) to heat resistance, and the acquisition method is as follows:

[0029] (1) Test strain: Bacillus amyloliquefaciens SDF-005 (CGMCC No. 6672);

[0030] (2) Preparation of culture medium plates: Melt potato dextrose agar (PDA) medium, pour into sterile culture dishes, cool, and prepare PDA plates; the PDA medium formula is: 200 g potatoes (peeled and cut into pieces), 20 g glucose, 14 g agar, and 1000 mL distilled water;

[0031] (3) Use an inoculation loop to pick a well-grown colony of Bacillus amyloliquefaciens SDF-005 and place it in NB culture medium. Shake and culture at 26°C and 160 r / min for 36 h. Pipette 1 mL of the bacterial solution and dissolve it in 9 mL of sterile water. After thorough shaking, place it in a water bath at 60°C, 70°C, 80°C, 85°C, 90°C, and 95°C for 30 min. Each temperature is a treatment, and each treatment is repeated 3 times. After cooling to room temperature (about 25°C), purify it by dilution separation to obtain a single colony strain. The strains obtained under different temperature treatments are numbered SDF-00560, SDF-00570, SDF-00580, SDF-00585, SDF-00590, and SF-00595.

[0032] (4) Using fig anthracnose as the target, the antibacterial activity was determined using the indoor confrontation culture method. The strain capacity was evaluated by comprehensively considering the heat resistance and antibacterial effect of the strains. A strain with strong inhibitory activity against fig anthracnose was screened and numbered SF-00595.

[0033] 2. Strain Identification

[0034] (1) Microbiological characteristics: The bacteria are rod-shaped, Gram-positive, with flagella around the body, and are facultatively anaerobic. The colonies grown on NA medium are round, white, flat, with a rough surface, a circular protrusion in the middle, and neat edges (see Figure 1 ). Liquid culture forms a biofilm, and the liquid is brown-yellow, turbid, and contains precipitation.

[0035] (2) Molecular biological characteristics:

[0036] The 16S rRNA gene sequence of the SF-00595 strain is shown in SEQ No. 1; gyrB The gene sequence determination result is shown in SEQ No.2.

[0037] The 16S rRNA amplified sequence of the SF-00595 strain and a housekeeping gene ( gyrB ) sequences were compared in the NCBI database, and the 16S rRNA sequences were compared with Bacillus amyloliquefaciens LT212 (CP000560) has a homology of 99.86%, gyrB Base and Bacillus amyloliquefaciens The homology of GXU-1 was 100%. Combined with the morphological characteristics of SF-00595 strain, it was determined that SF-00595 belongs to Bacillus amyloliquefaciens ( Bacillus amyloliquefaciens ).

[0038] This strain was deposited in the China Center for Type Culture Collection (CCTCC) on April 21, 2025, address: Wuhan University, China Center for Type Culture Collection, Wuhan, China, with the deposit number CCTCC NO: M 2025830.

[0039] Example 2 Indoor Verification of the Effect of Bacillus amyloliquefaciens SF-00595 on Fig Anthracnose and Papaya Anthracnose

[0040] 1. Experimental Methods

[0041] The plate confrontation culture method was used to culture the fig anthracnose pathogen ( Colletotrichum enigma )、Papaya Anthracnose ( Colletotrichum fioriniae) a 5 mm bacterial cake was transferred to the center of the plate. Bacillus amyloliquefaciens SF-00595 was inoculated symmetrically 2 cm from the cake and incubated in a 28°C biochemical incubator. Each treatment was replicated three times. When the mycelium of the control group (inoculated only with a 5 mm anthracnose cake in the center of the plate) filled the plate, the colony diameter and inhibition zone diameter were measured using the cross-hatch method, and the average and relative inhibition rate were calculated.

[0042] 2. Results

[0043] From Table 1 and Figure 2 、 Figure 3 It can be seen that Bacillus amyloliquefaciens SF-00595 has a strong inhibitory effect on the mycelial growth of fig anthracnose and papaya anthracnose, with the inhibition zones being 12.6 mm and 11.9 mm, respectively, and the mycelial growth inhibition rates being 81.5% and 80.0%, respectively.

[0044] Table 1 Indoor antibacterial activity of Bacillus amyloliquefaciens SF-00595 against fig anthracnose and papaya anthracnose

[0045]

[0046] Example 3 Preparation of Bacillus amyloliquefaciens SF-00595 bacterial agent

[0047] The seed liquid culture medium is (weight percentage): soybean meal 3.2%, corn starch 2.2%, glucose 0.5%, manganese sulfate 0.02%, and the rest is water, pH 7.0-7.5.

[0048] The fermentation medium is (percentage by weight): soybean meal 5.9%, corn starch 4.8%, glucose 0.5%, manganese sulfate 0.02%, and the rest is water, with a pH of 7.0-7.5.

[0049] The preparation method comprises the following steps:

[0050] 1) Shaking culture: Pick a small number of Bacillus amyloliquefaciens SF-00595 colonies from the slant of the test tube, transfer them to the seed medium, and culture them in a shaking incubator at 30°C for 12-14 hours.

[0051] 2) Seed liquid culture: Add the shaker cultured bacterial liquid into a seed tank (250L) filled with seed liquid culture medium and culture at 37°C, initial pH 7.0-7.5, and ventilation volume 15m 3 / h, culture time 12-14h;

[0052] 3) Fermentation culture: Transfer the seed liquid into the fermentation medium for culture, culture temperature 37℃, initial pH 7.0-7.5, liquid volume 70%, inoculation volume 10%, culture time 24h, ventilation volume 220m3 / h, using this culture medium for fermentation, the bacterial count can reach 100 billion / g;

[0053] 4) Amount of auxiliary materials: Add water-soluble carrier industrial glucose at a rate of 10% of the fermentation liquid mass and stir evenly in the batching tank;

[0054] 5) Spray drying: air inlet 170℃, air outlet 75-70℃;

[0055] 6) Compounding: Pour the spray-dried bacterial powder into a mixer, add the water-soluble carrier industrial glucose, and stir thoroughly for 30 minutes until evenly mixed to prepare a bacterial agent with a live bacterial content of 60 billion / g.

[0056] Example 4 Verification of the Effect of Bacillus amyloliquefaciens SF-00595 on Fig Anthracnose

[0057] This example provides experiments related to the activity of Bacillus amyloliquefaciens SF-00595 against fig anthracnose.

[0058] 1. Methods

[0059] 1.1 Test agent: Bacillus amyloliquefaciens SF-00595 bacterial agent (active bacterial content 60 billion / g).

[0060] 1.2 Test crops and control targets:

[0061] The test crop was fig (Green Early) and the target disease was anthracnose. Fig leaves of uniform size and health, unexposed to the pesticide, were collected from the experimental base. They were rinsed three times with sterile water, air-dried, and placed in a fresh-keeping box (16 cm × 10 cm × 6 cm) containing soaked filter paper until ready for use.

[0062] 1.3 Methods

[0063] 1.3.1 Determination of preventive effect

[0064] Using a hand sprayer (E532), Bacillus amyloliquefaciens SF-00595 inoculum (100-fold, 200-fold, and 300-fold dilutions) was sprayed on the surface of fig leaves to form a uniform film. Fig leaves sprayed with water served as a control. 24 h after treatment, a sterilized inoculation needle was used to slightly puncture the surface of the fig leaves to form a microwound. 100 μL of anthracnose spore suspension (10 6Spores / mL) were dropped on the wound. 15 leaves were treated for each time, and each leaf was inoculated at one place. The inoculated leaves were placed in an incubator (25℃; L﹕D = 12 h﹕12 h; relative humidity above 85%) for incubation and the disease development was observed. After 7 days of incubation, the diameter of the lesions was measured and the average value was taken to calculate the prevention effect. The experiment was repeated 4 times.

[0065] 1.3.2 Determination of treatment efficacy

[0066] Fig leaves collected for the experiment were first inoculated with an anthracnose spore suspension according to the above method. 24 hours later, the leaves were sprayed with the same concentrations (100x, 200x, and 300x dilutions) as described above. Control leaves were sprayed with water. Each treatment consisted of 15 leaves, with one inoculation site per leaf. The experimental figs were incubated in an incubator (25°C; L / D = 12 h / 12 ​​h; relative humidity above 85%) and observed for disease development. After 7 days of incubation, the diameter of the lesions was measured and the average value was used to calculate the control efficacy. The experiment was repeated four times.

[0067] 1.4 Statistics

[0068] Control effect (%) = (control lesion diameter - treatment lesion diameter) / control lesion diameter × 100.

[0069] 2. Results

[0070] 2.1 Preventive effect of Bacillus amyloliquefaciens SF-00595 on fig anthracnose

[0071] The results in Table 2 show that the Bacillus amyloliquefaciens SF-00595 agent has a good preventive effect on fig anthracnose, with the preventive effects of 100 times, 200 times, and 300 times reaching 78.95%, 73.49%, and 62.57%, respectively.

[0072] Table 2 Preventive effect of Bacillus amyloliquefaciens SF-00595 on fig anthracnose

[0073]

[0074] 2.2 Therapeutic effect of Bacillus amyloliquefaciens SF-00595 on fig anthracnose

[0075] The results in Table 3 show that the Bacillus amyloliquefaciens SF-00595 agent has a good therapeutic effect on fig anthracnose, with the therapeutic effects of 100 times, 200 times, and 300 times reaching 68.84%, 65.96%, and 64.40%, respectively.

[0076] Table 3 The therapeutic effect of Bacillus amyloliquefaciens SF-00595 on fig anthracnose

[0077]

[0078] 3. Summary

[0079] Bacillus amyloliquefaciens SF-00595 has a strong preventive and therapeutic effect against fig anthracnose. The preventive effect of treatments with 100-fold and 200-fold dilutions was greater than its therapeutic effect, and the preventive effect increased with increasing agent concentration. Treatments with a 300-fold dilution had a preventive effect comparable to the therapeutic effect. Treatments with 100-fold and 200-fold dilutions achieved preventive effects of 78.95% and 73.49%, respectively, while therapeutic effects reached 68.84% and 65.96%, respectively. All three treatments demonstrated strong preventive and therapeutic effects against fig anthracnose, effectively controlling the damage caused by the disease.

[0080] Example 5: Verification of the effect of Bacillus amyloliquefaciens SF-00595 on papaya anthracnose

[0081] This example provides experiments related to the use of the Bacillus amyloliquefaciens SF-00595 agent against papaya anthracnose.

[0082] 1. Methods

[0083] 1.1 Test agent: Bacillus amyloliquefaciens SF-00595 bacterial agent (active bacterial content 60 billion / g).

[0084] 1.2 Test crops and control targets: Test crop: papaya; control target: anthracnose.

[0085] Papaya fruits of the same size and health that had not been exposed to the pesticide were collected from the experimental site. They were rinsed three times with sterile water, dried, and placed in a beaker (1 L) containing soaked filter paper for later use.

[0086] 1.3 Test methods

[0087] 1.3.1 Determination of preventive effect

[0088] Using a hand sprayer (E532), Bacillus amyloliquefaciens SF-00595 inoculum (100-fold, 200-fold, and 300-fold dilutions) was sprayed on the surface of papaya fruit to form a uniform film. Papaya fruit sprayed with water was used as a control. 24 h after treatment, a sterilized inoculation needle was used to slightly puncture the surface of the papaya fruit to form a microwound. 100 μL of anthracnose spore suspension (10 6 Spores / mL) were dropped onto the wound. Ten fruits were used for each treatment, and one spot was inoculated on each fruit. The inoculated fruits were placed in a sterile beaker (1 L) (25 ℃; L﹕D = 12 h﹕12 h; relative humidity above 85%) for incubation and the disease development was observed. After 7 days of incubation, the diameter of the lesions was measured and the average value was taken to calculate the prevention efficacy. The experiment was repeated 4 times.

[0089] 1.3.2 Determination of treatment efficacy

[0090] Papaya fruits collected for the experiment were first inoculated with an anthracnose spore suspension according to the above method. 24 hours later, the same concentrations (100x, 200x, and 300x dilutions) of the anthracnose spore suspension were sprayed on the leaves of the control group. Ten fruits were inoculated at one site per treatment. The experimental papayas were placed in a sterile beaker (1 L) (25°C; L / D = 12 h / 12 ​​h; relative humidity above 85%) and observed for disease development. After 7 days of incubation, the diameter of the lesions was measured and the average value was used to calculate the control efficacy. The experiment was repeated four times.

[0091] 1.4 Data Statistics

[0092] Same as Example 4.

[0093] 2. Results

[0094] 2.1 Preventive effect of Bacillus amyloliquefaciens SF-00595 on papaya anthracnose

[0095] The results in Table 4 show that the Bacillus amyloliquefaciens SF-00595 agent has a good preventive effect on papaya anthracnose, with the preventive effects of 100 times, 200 times, and 300 times reaching 77.72%, 75.02%, and 72.45%, respectively.

[0096] Table 4 The preventive effect of Bacillus amyloliquefaciens SF-00595 on papaya anthracnose

[0097]

[0098] 2.2 Therapeutic effect of Bacillus amyloliquefaciens SF-00595 on papaya anthracnose

[0099] The results in Table 5 show that the Bacillus amyloliquefaciens SF-00595 agent has a good therapeutic effect on papaya anthracnose, with the therapeutic effects of 100 times, 200 times, and 300 times reaching 68.64%, 66.05%, and 63.34%, respectively.

[0100] Table 5 The therapeutic effect of Bacillus amyloliquefaciens SF-00595 on papaya anthracnose

[0101]

[0102] 3. Summary

[0103] The preventive effect of all three treatments with the Bacillus amyloliquefaciens SF-00595 inoculant was greater than its therapeutic effect, and the effect increased with increasing inoculant concentration. All three treatments demonstrated significant preventive efficacy (over 70% for all three treatments) and therapeutic efficacy (over 60% for all three treatments) against papaya anthracnose, effectively controlling the damage caused by papaya anthracnose.

Claims

1. Bacillus amyloliquefaciens ( Bacillus amyloliquefaciens ) SF-00595, the deposit number of the strain is CCTCC NO: M 2025830.

2. A biological agent comprising the Bacillus amyloliquefaciens SF-00595 according to claim 1 as an active ingredient.

3. The method for preparing the biological agent according to claim 2, wherein: The following steps are involved: 1) Shaking culture: Pick a colony of Bacillus amyloliquefaciens SF-00595 from the slant of the test tube, transfer it to the seed medium, and culture it in a shaking incubator at 28-32°C for 12-14 hours. 2) Seed liquid culture: Add the shaker-cultured bacterial solution to a seed tank containing seed liquid culture medium and culture at a temperature of 35-40°C, an initial pH of 7.0-7.5, and a culture time of 12-14 hours to obtain seed liquid; 3) Fermentation culture: The seed liquid is transferred to the fermentation medium for culture to obtain the fermentation liquid. The culture temperature is 35-40℃, the initial pH is 7.0-7.5, the inoculation amount is 3-10%, and the culture time is 20-30h. The fermentation liquid is then further made into a biological agent.

4. The method for preparing the biological agent according to claim 3, wherein: The seed liquid culture medium comprises, by weight percentage, 3.2% soybean meal powder, 2.2% corn starch, 0.5% glucose, 0.02% manganese sulfate, and the remainder water, with a pH of 7.0-7.

5.

5. The method for preparing the biological agent according to claim 3, wherein: The fermentation medium comprises, by weight percentage, 5.9% soybean meal powder, 4.8% corn starch, 0.5% glucose, 0.02% manganese sulfate, and the remainder water, with a pH of 7.0-7.

5.

6. The method for preparing the biological agent according to claim 3, wherein: The fermentation liquid is added to a water-soluble carrier and spray-dried, the spray-dried bacterial powder is poured into a mixer, and the water-soluble carrier is continuously added and stirred evenly to obtain a biological bacterial agent.

7. The Bacillus amyloliquefaciens SF-00595 of claim 1 or the biological agent of claim 2 for controlling pathogens Colletotrichum fioriniae Uses for papaya anthracnose caused by.

8. The Bacillus amyloliquefaciens SF-00595 of claim 1 or the biological agent of claim 2 for controlling pathogens Colletotrichum aenigma Uses for fig anthracnose caused by.

9. A method for preventing and controlling papaya anthracnose or fig anthracnose, characterized in that: The papaya anthracnose is caused by the pathogen Colletotrichum fioriniae Papaya anthracnose caused by the fig anthracnose pathogen Colletotrichum aenigma Fig anthracnose caused by the disease; the method comprises applying the Bacillus amyloliquefaciens SF-00595 biological agent according to claim 2 to the papaya or fig with anthracnose.

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