Soil soluble organic matter analysis method based on Fourier transform mass spectrometry

Abstract

The invention discloses a soil soluble organic matter analysis method based on Fourier transform mass spectrometry, and relates to the technical field of soil analysis, and the method comprises the following steps: sampling a soil area; obtaining soil organic matters by using a centrifugal machine; obtaining a conventional peak value based on Fourier transform mass spectrometry; dissolving characteristics are obtained through soluble stripping treatment, and an essential peak value is obtained; acquiring distribution data of soluble organic matters of the soil based on the essential peak value; the method is used for solving the problems that in an existing soil soluble organic matter analysis method, soluble organic matters and insoluble organic matters in organic matters cannot be distinguished during analysis, so that whether the soluble organic matters exist in the soil cannot be judged, and the content of the soluble organic matters in the soil cannot be obtained; the treatment efficiency of the organic matters in the soil is influenced.

Description

Technical Field

[0001] The present invention relates to the technical field of soil analysis, and specifically to a method for analyzing soil soluble organic matter based on Fourier transform mass spectrometry. Background Art

[0002] Soil organic matter refers to all carbon-containing organic substances existing in the soil in various forms, including various animal and plant residues, microorganisms in the soil, and various organic substances decomposed and synthesized by them; soil organic matter is an important component of the solid phase of the soil and one of the main sources of plant nutrition. It can promote the growth and development of plants, improve the physical properties of the soil, promote the activities of microorganisms and soil organisms, promote the decomposition of nutrient elements in the soil, and improve the fertilizer retention and buffering properties of the soil.

[0003] Existing methods for analyzing soil soluble organic matter usually collect environmental data of the soil, obtain spectral characteristics by acquiring remote sensing data, and establish an estimation model in combination with existing data. The estimation model is trained to analyze the organic matter in the soil and obtain the distribution result of soil organic matter. Although this improved method can quickly detect the content of organic matter in the soil, when analyzing the soluble organic matter in the soil, it is impossible to distinguish the soluble organic matter and insoluble organic matter in the organic matter only through environmental data and spectral characteristics. As a result, when analyzing the organic matter in the soil, it is impossible to determine whether there is soluble organic matter in the soil and impossible to obtain the content of soluble organic matter in the soil, thus leading to the problem of affecting the processing efficiency of organic matter in the soil. For example, in the patent application with the publication number CN118823602A, a method and system for identifying and analyzing vineyard soil organic matter based on machine learning are disclosed. This solution trains an initial organic matter estimation model through environmental data, spectral characteristics, and actual measurement of organic matter to obtain an organic matter estimation model; based on the organic matter evaluation model, the vineyard soil is evaluated and analyzed for organic matter to obtain the distribution result of vineyard organic matter. Other methods for analyzing soil soluble organic matter usually analyze the relationship between the content of organic matter and the content of a certain element, and still cannot distinguish the soluble organic matter and insoluble organic matter in the organic matter during analysis. As a result, when analyzing the organic matter in the soil, it is impossible to determine whether there is soluble organic matter in the soil and impossible to obtain the content of soluble organic matter in the soil, thus leading to the problem of affecting the processing efficiency of organic matter in the soil. In view of this, it is necessary to improve the existing methods for analyzing soil soluble organic matter. Summary of the Invention

[0004] The present invention aims to solve at least one of the technical problems in the prior art to some extent. By proposing a method for analyzing soil soluble organic matter based on Fourier transform mass spectrometry, it is used to solve the problem that in the existing analysis methods for soil soluble organic matter, it is impossible to distinguish soluble organic matter and insoluble organic matter in organic matter during analysis. As a result, when analyzing the organic matter in soil, it is impossible to determine whether there is soluble organic matter in the soil and impossible to obtain the content of soluble organic matter in the soil, thus affecting the treatment efficiency of organic matter in the soil.

[0005] To achieve the above object, the present application provides a method for analyzing soil soluble organic matter based on Fourier transform mass spectrometry, including the following steps:

[0006] Denote the area where the soil to be analyzed is located as the soil area; sample the soil area, and denote the sampled soil as the sampling soil; use a centrifuge to separate the sampling soil to obtain the organic matter contained in each sampling soil, and denote it as soil organic matter;

[0007] Analyze the sampling soil using the organic matter analysis method based on Fourier transform mass spectrometry, and obtain the conventional mass spectrum and conventional peaks corresponding to each sampling soil based on the analysis results; perform soluble stripping treatment on each sampling soil, and obtain the dissolution characteristics corresponding to each sampling soil based on the treatment results;

[0008] Analyze all the conventional peaks and dissolution characteristics, and obtain the essential peaks corresponding to all the sampling soils based on the analysis results;

[0009] Analyze the soluble organic matter in the soil in the soil area based on the essential peaks, and obtain the distribution data of the soluble organic matter in the soil based on the analysis results.

[0010] Further, the organic matter analysis method includes:

[0011] For any sampling soil, establish a database and denote it as the organic matter record library; obtain all the types of soil organic matter in the sampling soil and store them in the organic matter record library. Among them, the organic matter record library includes a soluble sub-library and an insoluble sub-library. Store the soluble organic matter stored in the organic matter record library in the soluble sub-library, and store the insoluble organic matter stored in the organic matter record library in the insoluble sub-library.

[0012] Further, the organic matter analysis method also includes:

[0013] Randomly obtain a sample from the sampled soil and denote it as the sample to be analyzed; ionize the sample to be analyzed using electrospray ionization and denote the ionized sample to be analyzed as the ion sample; introduce the ion sample into a strong magnetic field, record the signal generated by the cyclotron motion of the ion sample based on the induction electrode, and convert the recorded signal into the frequency domain, denoted as the sample frequency domain;

[0014] Process the sample frequency domain based on Fourier transform and obtain the corresponding mass spectrum based on the distribution of mass-to-charge ratio obtained from the processing, denoted as the conventional mass spectrum; denote the point with the largest ordinate in the conventional mass spectrum as the sample peak point, and denote the abscissa of the sample peak point as the conventional peak of the sampled soil.

[0015] Furthermore, perform soluble stripping treatment on each sampled soil and obtain the dissolution characteristics corresponding to each sampled soil based on the treatment results, including:

[0016] Analyze all sampled soils based on the organic matter analysis method and obtain the conventional peak corresponding to each sampled soil;

[0017] For any sampled soil, the soluble stripping treatment includes: denote all the organic matter in the soluble sub-library corresponding to the sampled soil as the organic matter to be dissolved, and denote the quantity of the organic matter to be dissolved as n; perform n random samplings on the sampled soil, and denote the sampled samples as the samples to be dissolved DR1 to the samples to be dissolved DR n ; sequentially denote all the organic matter to be dissolved as the dissolution objects of the n samples to be dissolved DR.

[0018] Furthermore, the soluble stripping treatment also includes:

[0019] For any sample to be dissolved DR, extract the dissolution object of the sample to be dissolved DR based on the dissolution conditions and dissolution method of the dissolution object of the sample to be dissolved DR, and analyze the sample to be dissolved DR with the extracted dissolution object based on the organic matter analysis method, and denote the obtained conventional peak as the dissolution peak of the dissolution object DR;

[0020] Obtain the dissolution peaks of all the samples to be dissolved DR of the sampled soil, and denote all the dissolution objects and dissolution peaks of the dissolved samples DR as the dissolution characteristics of the sampled soil; obtain the dissolution characteristics of all sampled soils.

[0021] Furthermore, analyze all the conventional peaks and dissolution characteristics, and obtain the essential peaks corresponding to all sampled soils based on the analysis results, including:

[0022] Obtain all the soluble organic matter in the soluble sub-library of all sampled soils, perform de-duplication processing on all the soluble organic matter, and sequentially denote all the soluble organic matter as the analyzed organic matter FX1 to the analyzed organic matter FX c, where c is a positive integer greater than or equal to n, and the duplicate removal process is to delete duplicate items;

[0023] For any analyzed organic matter FX, the sampled soils in which the analyzed organic matter FX is recorded as the dissolved object in all the dissolution characteristics of the sampled soils are successively recorded as the soils to be analyzed DT1 to the soils to be analyzed DT m , for any soil to be analyzed DT u , the soil to be analyzed DT u In the dissolution characteristics of, the dissolution peak corresponding to the analyzed organic matter FX as the dissolved object is denoted as α1, and the conventional peak of the soil to be analyzed DT u is denoted as α2, where u is a positive integer less than or equal to m and greater than or equal to 1.

[0024] Furthermore, analyzing all the conventional peaks and dissolution characteristics, and obtaining the essential peaks corresponding to all the sampled soils based on the analysis results further includes:

[0025] Denote the difference between α2 and α1 as the analysis difference of the soil to be analyzed DT u ; obtain the analysis differences of all the soils to be analyzed DT, and denote the average value of all the analysis differences as the discrimination value of the analyzed organic matter FX;

[0026] Obtain the discrimination values of all the analyzed organic matter FX.

[0027] Furthermore, analyzing all the conventional peaks and dissolution characteristics, and obtaining the essential peaks corresponding to all the sampled soils based on the analysis results further includes:

[0028] For any sampled soil, randomly obtain a sample in the sampled soil and denote it as the essential sample, and use the essential acquisition method to obtain the essential peak of the essential sample. The essential acquisition method is: based on the dissolution conditions and dissolution methods of all the soluble organic matters in the soluble sub-library of the sampled soil, extract all the soluble organic matters in the essential sample, and analyze the essential sample of all the extracted soluble organic matters based on the organic matter analysis method, and denote the obtained conventional peak as the sample peak of the essential sample;

[0029] Based on the sample peak, use the soluble solute adjustment algorithm to obtain the essential peak of the essential sample. The soluble solute adjustment algorithm is: where F is the essential peak, f0 is the sample peak, F0 is the conventional peak of the sampled soil, g i is the discrimination value of the i-th soluble organic matter in the soluble sub-library of the sampled soil, and k is the number of soluble organic matters in the soluble sub-library of the sampled soil;

[0030] Obtain the essential peaks corresponding to all the sampled soils.

[0031] Further, analyze the soluble organic matter of the soil within the soil area based on the essential peak, and obtain the distribution data of the soluble organic matter of the soil based on the analysis results, including:

[0032] For any sampled soil, record the difference between the conventional peak and the essential peak of the sampled soil as the soluble difference of the sampled soil; obtain the soluble differences corresponding to all sampled soils;

[0033] When analyzing the soil within the soil area, for any soil to be analyzed, randomly obtain two samples of the soil to be analyzed, and record them as the sample to be analyzed A and the sample to be analyzed B in sequence. Process the sample to be analyzed A based on the organic matter analysis method, and record the conventional peak corresponding to the sample to be analyzed A as the conventional sample peak;

[0034] Obtain the dissolution conditions and dissolution methods for all soluble organic matter, extract all the soluble organic matter in the sample to be analyzed B, and analyze the sample to be analyzed B with all the soluble organic matter extracted based on the organic matter analysis method, and record the obtained conventional peak as the essential sample peak.

[0035] Further, analyze the soluble organic matter of the soil within the soil area based on the essential peak, and obtain the distribution data of the soluble organic matter of the soil based on the analysis results, which also includes:

[0036] Record the difference between the conventional sample peak and the essential sample peak as the value of the sample to be analyzed; respectively obtain the differences between the soluble differences corresponding to all sampled soils and the value of the sample to be analyzed, and record the sampled soil corresponding to the smallest difference as the representative soil; when the number of representative soils is 1, record all the soluble organic matter in the soluble sub-library of the representative soil as the soluble organic matter of the soil to be analyzed; when the number of representative soils is greater than 1, record the soluble organic matter that exists in the soluble sub-libraries of all representative soils as the soluble organic matter of the soil to be analyzed.

[0037] Advantages of the present invention: In this application, the area where the soil to be analyzed is located is first denoted as the soil area; the soil in the soil area is sampled, and the sampled soil is denoted as the sampled soil; a centrifuge is used to separate the sampled soil to obtain the organic matter contained in each sampled soil, which is denoted as soil organic matter; then, based on Fourier transform mass spectrometry, the organic matter analysis method is used to analyze the sampled soil, and based on the analysis results, the corresponding conventional mass spectrum and conventional peaks of each sampled soil are obtained. The advantage of this is that by using a centrifuge to separate the sampled soil and obtain soil organic matter, and then analyzing the sampled soil based on Fourier transform mass spectrometry using the organic matter analysis method, the mass spectrum corresponding to the organic matter in the sampled soil can be accurately obtained, which helps to obtain the relative content of soluble organic matter in the sampled soil based on the mass spectrum corresponding to the organic matter in the sampled soil during subsequent analysis, and provides a data basis for the subsequent analysis of soluble organic matter, ensuring that it can be determined whether there is soluble organic matter in the soil and the content of soluble organic matter in the soil during analysis, so as to solve the problem of affecting the processing efficiency of organic matter in the soil due to the inability to obtain the content of soluble organic matter in the soil.

[0038] Also, by performing soluble stripping treatment on each sampled soil and obtaining the dissolution characteristics corresponding to each sampled soil based on the treatment results; analyzing all the conventional peaks and dissolution characteristics, and obtaining the essential peaks corresponding to all the sampled soils based on the analysis results; finally, analyzing the soluble organic matter in the soil in the soil area based on the essential peaks, and obtaining the distribution data of the soluble organic matter in the soil based on the analysis results. The advantage of this is that by obtaining the essential peaks corresponding to the sampled soil, the point with the largest mass-to-charge ratio of the organic matter in the sampled soil after removing the soluble organic matter can be obtained, which helps to judge whether there is soluble organic matter in the soil, obtain the content and composition of the soluble organic matter in the soil during subsequent analysis, so as to ensure that accurate and effective distribution data of the soluble organic matter in the soil are provided for the staff and improve the processing efficiency of the organic matter in the soil. BRIEF DESCRIPTION OF THE DRAWINGS

[0039] Figure 1 is the step flow chart of the method of the present invention;

[0040] Figure 2 is the flow schematic diagram of the soluble stripping treatment of the present invention;

[0041] Figure 3 is the structural schematic diagram of the electronic device of the present invention. DETAILED DESCRIPTION OF THE INVENTION

[0042] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0043] Example 1. Please refer to Figure 1 As shown, the present application provides a method for analyzing soil soluble organic matter based on Fourier transform mass spectrometry, including the following steps:

[0044] Step S1: Denote the area where the soil to be analyzed is located as the soil area; sample the soil area, and denote the sampled soil as the sampled soil; use a centrifuge to separate the sampled soil to obtain the organic matter contained in each sampled soil, and denote it as soil organic matter.

[0045] In the specific implementation process, the purpose of using a centrifuge in this embodiment is to extract the organic matter in the sampled soil. During actual analysis, the centrifuge can be replaced according to the actual available tools, and the soil organic matter for actual analysis can be obtained;

[0046] Step S2: Analyze the sampled soil using the organic matter analysis method based on Fourier transform mass spectrometry, and obtain the corresponding conventional mass spectrum and conventional peak for each sampled soil based on the analysis results; perform soluble stripping treatment on each sampled soil, and obtain the dissolution characteristics corresponding to each sampled soil based on the treatment results;

[0047] Analyze all the conventional peaks and dissolution characteristics, and obtain the essential peaks corresponding to all the sampled soils based on the analysis results;

[0048] Step S201: The organic matter analysis method includes: Step S2011: For any sampled soil, establish a database and denote it as the organic matter record library; obtain all the types of soil organic matter in the sampled soil and store them in the organic matter record library. Among them, the organic matter record library includes a soluble sub-library and an insoluble sub-library. Store the soluble organic matter stored in the organic matter record library in the soluble sub-library, and store the insoluble organic matter stored in the organic matter record library in the insoluble sub-library;

[0049] In the specific implementation process, the soluble organic matter in the soil may include easily soluble or decomposable organic matter such as humic acid, and the insoluble organic matter in the soil includes humin and other organic matter that is not easily decomposed;

[0050] Step S2012, randomly obtain a sample from the sampled soil and denote it as the sample to be analyzed; ionize the sample to be analyzed using electrospray ionization, and denote the ionized sample to be analyzed as the ion sample; introduce the ion sample into a strong magnetic field, record the signal generated by the cyclotron motion of the ion sample based on the induction electrode, and convert the recorded signal into the frequency domain, denoted as the sample frequency domain;

[0051] In the specific implementation process, the purpose of using electrospray ionization is to ionize the sample to be analyzed. In actual applications, existing tools can be used to process the sample to be analyzed to achieve the ionization of the sample to be analyzed, such as matrix-assisted laser desorption / ionization;

[0052] Process the sample frequency domain based on Fourier transform, and obtain the corresponding mass spectrum based on the distribution of mass-to-charge ratio obtained from the processing, denoted as the conventional mass spectrum; denote the point with the largest ordinate in the conventional mass spectrum as the sample peak point, and denote the abscissa of the sample peak point as the conventional peak of the sampled soil. In the specific implementation process, by obtaining the mass spectrum corresponding to the sample to be analyzed based on Fourier transform mass spectrometry, the mass-to-charge ratio corresponding to all ions in the sampled soil can be accurately obtained, which helps to provide data support for subsequent analysis, so as to obtain the relevant parameters corresponding to the soluble organic matter in the soil.

[0053] Step S2 further includes: Step S202, analyze all the sampled soils based on the organic matter analysis method, and obtain the conventional peak corresponding to each sampled soil;

[0054] Step S203, for any sampled soil, the soluble stripping treatment includes, please refer to Figure 2 as shown: Step S2031, denote all the organic matter in the soluble sub-library corresponding to the sampled soil as the organic matter to be dissolved, and denote the quantity of the organic matter to be dissolved as n; perform n random samplings on the sampled soil, and denote the sampled samples as the samples to be dissolved DR1 to the samples to be dissolved DR n ; sequentially denote all the organic matter to be dissolved as the dissolution objects of n samples to be dissolved DR;

[0055] Step S2032, for any sample to be dissolved DR, based on the dissolution conditions and dissolution methods of the dissolution object of the sample to be dissolved DR, extract the dissolution object of the sample to be dissolved DR, and analyze the sample to be dissolved DR with the extracted dissolution object based on the organic matter analysis method, and denote the obtained conventional peak as the dissolution peak of the dissolution object DR;

[0056] In the specific implementation process, for example, if the dissolution object in the sample to be dissolved DR during analysis is humic acid, the humic acid in the humic acid can be dissolved by dilute alkali to achieve the purpose of extracting the dissolution object in the sample to be dissolved DR; by extracting the dissolution object of the sample to be dissolved DR, the conventional peak corresponding to the mass spectrum of the sampling soil without the sample to be dissolved DR can be obtained, so that the discrimination value corresponding to each dissolution object can be obtained by combining the conventional peak of the sampling soil during subsequent analysis, so as to achieve the purpose of analyzing the content and type of soluble organic matter in the soil in the future;

[0057] Step S2033, obtain the dissolution peaks of all samples to be dissolved DR of the sampling soil, and record the dissolution objects and dissolution peaks of all dissolved samples DR as the dissolution characteristics of the sampling soil; obtain the dissolution characteristics of all sampling soils.

[0058] Step S2 further includes: Step S204, obtain all soluble organic matters in the soluble sub-library of all sampling soils, and after removing duplicates from all soluble organic matters, record all soluble organic matters as analytical organic matter FX1 to analytical organic matter FX c , where c is a positive integer greater than or equal to n, and the duplicate removal process is to delete duplicate items;

[0059] Step S205, for any analytical organic matter FX, record the sampling soils in which the analytical organic matter FX is recorded as the dissolution object in the dissolution characteristics of all sampling soils as the soils to be analyzed DT1 to the soils to be analyzed DT m , for any soil to be analyzed DT u , the soil to be analyzed DT u In the dissolution characteristics of, record the dissolution peak corresponding to the analytical organic matter FX as α1, and record the conventional peak of the soil to be analyzed DT u as α2, where u is a positive integer less than or equal to m and greater than or equal to 1;

[0060] Step S206, record the difference between α2 and α1 as the analysis difference of the soil to be analyzed DT u ; obtain the analysis differences of all soils to be analyzed DT, and record the average value of all analysis differences as the discrimination value of the analytical organic matter FX;

[0061] In the specific implementation process, for example, during a data processing, the conventional peak of the soil DT to be analyzed is 80%. In the dissolution characteristics of the soil DT to be analyzed, the dissolution object is the dissolution peak corresponding to the analyzed organic matter FX, which is 70%. Then, the analysis difference of the soil to be analyzed can be recorded as 10%. By analyzing all the soil DT to be analyzed, the obtained analysis differences are 10%, 9%, 7%, 10%, and 11% respectively. Then, the discrimination value of the analyzed organic matter FX can be set to 9.4%. By obtaining the discrimination value of the analyzed organic matter FX, the corresponding data characteristics of each soluble organic matter in the soil in the mass spectrum can be obtained, which helps to analyze the content and types of soluble organic matter in the soil during subsequent analysis;

[0062] Step S207: Obtain the discrimination values of all the analyzed organic matter FX;

[0063] Step S208: For any sampled soil, randomly obtain a sample in the sampled soil and record it as the essential sample. Use the essential acquisition method to obtain the essential peak of the essential sample. The essential acquisition method is as follows: Based on the dissolution conditions and dissolution methods of all soluble organic matters in the soluble sub-library of the sampled soil, extract all the soluble organic matters in the essential sample, and analyze the essential sample of all the extracted soluble organic matters based on the organic matter analysis method, and record the obtained conventional peak as the sample peak of the essential sample;

[0064] Step S209: Use the solute adjustment algorithm to obtain the essential peak of the essential sample based on the sample peak. The solute adjustment algorithm is as follows: where F is the essential peak, f0 is the sample peak, F0 is the conventional peak of the sampled soil, and g i is the discrimination value of the i-th soluble organic matter in the soluble sub-library of the sampled soil, and k is the number of soluble organic matters in the soluble sub-library of the sampled soil;

[0065] In the specific implementation process, for example, during a data analysis, the obtained sample peak is 60%, the conventional peak is 95%, and the discrimination values of k soluble organic matters are 9.5%, 10%, 11.5%, 10%, 10%, 10%, and 10% respectively. Then, through calculation, the essential peak is 96%. By using the solute adjustment algorithm, the sample peak can be calibrated through the discrimination values of the soluble organic matters in the sampled soil, so that the obtained essential peak is more in line with the data parameters during actual analysis;

[0066] Step S210: Obtain the essential peaks corresponding to all the sampled soils.

[0067] Step S3: Analyze the soluble organic matter in the soil in the soil area based on the essential peak, and obtain the distribution data of the soluble organic matter in the soil based on the analysis results;

[0068] Step S3 includes: Step S301, for any sampled soil, record the difference between the conventional peak value and the essential peak value of the sampled soil as the soluble difference of the sampled soil; obtain the soluble differences corresponding to all sampled soils;

[0069] Step S302, when analyzing the soil within the soil area, for any soil to be analyzed, randomly obtain two samples of the soil to be analyzed, and sequentially denote them as the sample to be analyzed A and the sample to be analyzed B. Process the sample to be analyzed A based on the organic matter analysis method, and denote the conventional peak value corresponding to the sample to be analyzed A as the conventional sample peak value;

[0070] Step S303, obtain the dissolution conditions and dissolution methods for all soluble organic matters, extract all soluble organic matters in the sample to be analyzed B, and analyze the sample to be analyzed B with all soluble organic matters extracted based on the organic matter analysis method, and denote the obtained conventional peak value as the essential sample peak value;

[0071] In the specific implementation process, for example, in a data processing, the obtained conventional sample peak value is 96%, and the essential sample peak value is 93%. Then the value of the sample to be analyzed is 3%. Among the soluble differences of all sampled soils obtained through analysis, only one soluble difference C1 has a difference of 0 from the value of the sample to be analyzed, and the differences between the other soluble differences and the sample to be analyzed are all greater than 0. This indicates that the content and distribution of the soluble organic matter in the sampled soil corresponding to the soluble difference C1 are relatively similar to those of the soil to be analyzed. Then, the sampled soil corresponding to the soluble difference C1 can be used as the representative soil, and the soluble organic matter of the soil to be analyzed can be recorded based on the soluble sub-library of the representative soil;

[0072] Step S304, record the difference between the conventional sample peak value and the essential sample peak value as the value of the sample to be analyzed; respectively obtain the differences between the soluble differences corresponding to all sampled soils and the value of the sample to be analyzed, and denote the sampled soil corresponding to the smallest difference as the representative soil; when the number of representative soils is 1, record all the soluble organic matters in the soluble sub-library of the representative soil as the soluble organic matter of the soil to be analyzed; when the number of representative soils is greater than 1, record the soluble organic matters that exist simultaneously in the soluble sub-libraries of all representative soils as the soluble organic matter of the soil to be analyzed.

[0073] Example 2, please refer to Figure 3 as shown in Figure 3The structural schematic diagram of an electronic device is exemplified. The electronic device may include: a processor, a communication interface, a memory, and a communication bus. Among them, the processor, the communication interface, and the memory complete communication with each other through the communication bus. The memory stores computer-readable instructions. The processor can call the instructions in the memory. When the computer-readable instructions are executed by the processor, the steps in a method for analyzing soil soluble organic matter based on Fourier transform mass spectrometry are run to achieve the following functions: First, mark the area where the soil to be analyzed is located as the soil area; sample the soil area, and mark the sampled soil as the sampled soil; use a centrifuge to separate the sampled soil to obtain the organic matter contained in each sampled soil, and mark it as soil organic matter; then analyze the sampled soil using the organic matter analysis method based on Fourier transform mass spectrometry, and obtain the conventional mass spectrum and conventional peaks corresponding to each sampled soil based on the analysis results; also perform soluble stripping treatment on each sampled soil, and obtain the dissolution characteristics corresponding to each sampled soil based on the treatment results; analyze all the conventional peaks and dissolution characteristics, and obtain the essential peaks corresponding to all the sampled soils based on the analysis results; finally, analyze the soluble organic matter in the soil area based on the essential peaks, and obtain the distribution data of the soluble organic matter in the soil based on the analysis results.

[0074] In addition, when the logical instructions in the above-mentioned memory are implemented in the form of software functional units and sold or used as independent products, they can be stored in a computer-readable storage medium. Based on such an understanding, the technical solution of the present application essentially or the part that contributes to the prior art or a part of the technical solution can be embodied in the form of a software product. The computer software product is stored in a storage medium and includes several instructions for causing a computer device (which can be a personal computer, a server, or a network device, etc.) to execute all or part of the steps of the methods described in various embodiments of the present application. And the aforementioned storage medium includes: various media such as USB flash drives, mobile hard disks, read-only memories (ROM, Read-Only Memory), random access memories (RAM, Random Access Memory), magnetic disks, or optical discs that can store program codes.

[0075] Embodiment 3. The present application further provides a computer program product. The computer program product includes a computer program stored on a computer-readable storage medium. The computer program includes program instructions. When the program instructions are executed by a computer, the computer can execute an analysis method of soil soluble organic matter based on Fourier transform mass spectrometry provided by each of the above methods. The method includes: First, mark the area where the soil to be analyzed is located as the soil area; sample the soil area, and mark the sampled soil as the sampled soil; use a centrifuge to separate the sampled soil to obtain the organic matter contained in each sampled soil, and mark it as soil organic matter; then analyze the sampled soil using the organic matter analysis method based on Fourier transform mass spectrometry, and obtain the corresponding conventional mass spectrum and conventional peaks for each sampled soil based on the analysis results; also perform soluble stripping treatment on each sampled soil, and obtain the corresponding dissolution characteristics for each sampled soil based on the treatment results; analyze all the conventional peaks and dissolution characteristics, and obtain the corresponding essential peaks for all the sampled soils based on the analysis results; finally, analyze the soluble organic matter in the soil area based on the essential peaks, and obtain the distribution data of the soluble organic matter in the soil based on the analysis results.

[0076] Embodiment 4. The present application further provides a computer-readable storage medium. The present application provides a storage medium on which a computer program is stored. When the computer program is executed by a processor, it runs the steps in an analysis method of soil soluble organic matter based on Fourier transform mass spectrometry as described above to achieve the following functions: First, mark the area where the soil to be analyzed is located as the soil area; sample the soil area, and mark the sampled soil as the sampled soil; use a centrifuge to separate the sampled soil to obtain the organic matter contained in each sampled soil, and mark it as soil organic matter; then analyze the sampled soil using the organic matter analysis method based on Fourier transform mass spectrometry, and obtain the corresponding conventional mass spectrum and conventional peaks for each sampled soil based on the analysis results; also perform soluble stripping treatment on each sampled soil, and obtain the corresponding dissolution characteristics for each sampled soil based on the treatment results; analyze all the conventional peaks and dissolution characteristics, and obtain the corresponding essential peaks for all the sampled soils based on the analysis results; finally, analyze the soluble organic matter in the soil area based on the essential peaks, and obtain the distribution data of the soluble organic matter in the soil based on the analysis results.

[0077] Through the description of the above embodiments, the embodiments of the present invention can be provided as a method, a system or a computer program product. Based on such understanding, the above technical solution, in essence or the part that contributes to the prior art, can be embodied in the form of a software product, and the computer software product can be stored in a computer-readable storage medium, such as ROM / RAM, magnetic disk, optical disk, etc., including several instructions for causing a computer device (which can be a personal computer, a server, or a network device, etc.) to execute the methods described in each embodiment or some parts of the embodiments.

[0078] In the embodiments provided in the present application, it should be understood that the disclosed system or method can be implemented in other ways. The above-described embodiments are merely illustrative. For example, the division of modules or units is only a logical function division, and there can be other division methods in actual implementation. For another example, multiple modules or units can be combined or integrated into another system, or some features can be ignored or not executed. Another point is that the displayed or discussed coupling or direct coupling or communication connection between each other can be through some communication interfaces, and the indirect coupling or communication connection of systems, modules and units can be in electrical, mechanical or other forms.

[0079] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present application and are not intended to limit them. Although the present application has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that they can still modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements for some of the technical features; and these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of each embodiment of the present application.

Claims

1. A method for analyzing soil soluble organic matter based on Fourier transform mass spectrometry, characterized in that, The steps include: Denote the area where the soil to be analyzed is located as the soil area; sample the soil area, and denote the sampled soil as the sampled soil; use a centrifuge to separate the sampled soil to obtain the organic matter contained in each sampled soil, and denote it as soil organic matter; Analyze the sampled soil using the organic matter analysis method based on Fourier transform mass spectrometry, and obtain the corresponding conventional mass spectrum and conventional peak for each sampled soil based on the analysis results; perform soluble stripping treatment on each sampled soil, and obtain the dissolution characteristics corresponding to each sampled soil based on the treatment results; Analyze all the conventional peaks and dissolution characteristics, and obtain the essential peaks corresponding to all the sampled soils based on the analysis results; Analyze the soluble organic matter in the soil area based on the essential peaks, and obtain the distribution data of the soluble organic matter in the soil based on the analysis results.

2. The method for analyzing soil soluble organic matter based on Fourier transform mass spectrometry according to claim 1, wherein, The organic matter analysis method includes: For any sampled soil, establish a database and denote it as the organic matter record library; obtain all the types of soil organic matter in the sampled soil and store them in the organic matter record library. Among them, the organic matter record library includes a soluble sub-library and an insoluble sub-library. Store the soluble organic matter stored in the organic matter record library in the soluble sub-library, and store the insoluble organic matter stored in the organic matter record library in the insoluble sub-library.

3. The method for analyzing soil soluble organic matter based on Fourier transform mass spectrometry according to claim 2, wherein The organic matter analysis method also includes: Randomly obtain a sample from the sampled soil and denote it as the sample to be analyzed; ionize the sample to be analyzed using electrospray ionization, and denote the ionized sample to be analyzed as the ion sample; introduce the ion sample into a strong magnetic field, record the signal generated by the cyclotron motion of the ion sample based on the induction electrode, and convert the recorded signal into the frequency domain, denoted as the sample frequency domain; Process the sample frequency domain based on Fourier transform, and obtain the corresponding mass spectrum based on the distribution of mass-to-charge ratio obtained by the processing, denoted as the conventional mass spectrum; denote the point with the largest ordinate in the conventional mass spectrum as the sample peak point, and denote the abscissa of the sample peak point as the conventional peak of the sampled soil.

4. A method for analyzing soil soluble organic matter based on Fourier transform mass spectrometry according to claim 3, characterized in that, Performing soluble stripping treatment on each sampled soil and obtaining the dissolution characteristics corresponding to each sampled soil based on the treatment results include: Analyze all the sampled soils using the organic matter analysis method, and obtain the conventional peak corresponding to each sampled soil; For any sampled soil, the soluble stripping treatment includes: recording all the organic matter in the soluble sub-library corresponding to the sampled soil as the organic matter to be dissolved, and recording the quantity of the organic matter to be dissolved as n; performing n random samplings on the sampled soil, and recording the sampled samples as the samples to be dissolved DR1 to the samples to be dissolved DR n ; sequentially recording all the organic matter to be dissolved as the dissolution objects of the n samples to be dissolved DR.

5. A method for analyzing soil soluble organic matter based on Fourier transform mass spectrometry according to claim 4, characterized in that, The soluble stripping treatment also includes: For any sample to be dissolved DR, extract the dissolution object of the sample to be dissolved DR based on the dissolution conditions and dissolution method of the dissolution object of the sample to be dissolved DR, and analyze the sample to be dissolved DR with the extracted dissolution object using the organic matter analysis method. Denote the obtained conventional peak as the dissolution peak of the dissolution object DR; Obtain the dissolution peaks of all the samples to be dissolved DR of the sampled soil, and denote the dissolution objects and dissolution peaks of all the dissolved samples DR as the dissolution characteristics of the sampled soil; obtain the dissolution characteristics of all the sampled soils.

6. The method for analyzing soil soluble organic matter based on Fourier transform mass spectrometry according to claim 5, wherein Analyzing all the conventional peaks and dissolution characteristics and obtaining the essential peaks corresponding to all the sampled soils based on the analysis results include: Obtain all the dissolved organic matter in the dissolved sub-library of all the sampled soils, and after removing duplicates from all the dissolved organic matter, sequentially record all the dissolved organic matter as analyzed organic matter FX1 to analyzed organic matter FX c , where c is a positive integer greater than or equal to n, and the duplicate removal process is to delete duplicate items; For any analyzed organic matter FX, the sampled soils in which the analyzed organic matter FX is recorded as the dissolved object in the dissolution characteristics of all sampled soils are successively recorded as the soils to be analyzed DT1 to the soils to be analyzed DT m , for any soil to be analyzed DT u , the soil to be analyzed DT u , in the dissolution characteristics of the soil to be analyzed DT u , the dissolution peak corresponding to the dissolved object being the analyzed organic matter FX is denoted as α1, and the conventional peak of the soil to be analyzed DT , where u is a positive integer less than or equal to m and greater than or equal to 1.

7. The method for analyzing soil soluble organic matter based on Fourier transform mass spectrometry according to claim 6, wherein, Analyze all conventional peaks and dissolution characteristics, and based on the analysis results, obtain the essential peaks corresponding to all sampled soils, including: Denote the difference between α2 and α1 as the soil DT to be analyzed u as the analysis difference; obtain the analysis differences of all soils DT to be analyzed, and denote the average value of all analysis differences as the discrimination value of the analyzed organic matter FX; Obtain the discrimination values of all analyzed organic matter FX.

8. A method for analyzing soil soluble organic matter based on Fourier transform mass spectrometry according to claim 7, characterized in that, Analyze all conventional peaks and dissolution characteristics, and based on the analysis results, obtain the essential peaks corresponding to all sampled soils, including: For any sampled soil, randomly obtain a sample in the sampled soil and denote it as the essential sample. Use the essential acquisition method to obtain the essential peak of the essential sample. The essential acquisition method is: based on the dissolution conditions and dissolution methods of all soluble organic matter in the soluble sub-library of the sampled soil, extract all soluble organic matter in the essential sample, and analyze the essential sample with all extracted soluble organic matter based on the organic matter analysis method. Denote the obtained conventional peak as the sample peak of the essential sample; Obtain the essential peak of the essential sample using the solute adjustment algorithm based on the sample peak. The solute adjustment algorithm is as follows: where F is the essential peak, f0 is the sample peak, F0 is the conventional peak of the sampled soil, and g i is the discrimination value of the i-th dissolved organic matter in the dissolved sub-library of the sampled soil, and k is the number of dissolved organic matters in the dissolved sub-library of the sampled soil; Obtain the essential peaks corresponding to all sampled soils.

9. The method for analyzing soil soluble organic matter based on Fourier transform mass spectrometry according to claim 8, characterized in that, Analyze the soluble organic matter in the soil area based on the essential peaks, and based on the analysis results, obtain the distribution data of the soluble organic matter in the soil, including: For any sampled soil, denote the difference between the conventional peak and the essential peak of the sampled soil as the soluble difference of the sampled soil; obtain the soluble differences corresponding to all sampled soils; When analyzing the soil in the soil area, for any soil to be analyzed, randomly obtain two samples of the soil to be analyzed and denote them as the sample to be analyzed A and the sample to be analyzed B in sequence. Process the sample to be analyzed A based on the organic matter analysis method, and denote the conventional peak corresponding to the sample to be analyzed A as the conventional sample peak; Obtain the dissolution conditions and dissolution methods for all soluble organic matter, extract all soluble organic matter in the sample to be analyzed B, and analyze the sample to be analyzed B with all extracted soluble organic matter based on the organic matter analysis method. Denote the obtained conventional peak as the essential sample peak.

10. The method for analyzing soil soluble organic matter based on Fourier transform mass spectrometry according to claim 9, wherein Analyze the soluble organic matter in the soil area based on the essential peaks, and based on the analysis results, obtain the distribution data of the soluble organic matter in the soil, including: Denote the difference between the conventional sample peak and the essential sample peak as the value of the sample to be analyzed; respectively obtain the differences between the soluble differences corresponding to all sampled soils and the value of the sample to be analyzed, and denote the sampled soil corresponding to the smallest difference as the representative soil; when the number of representative soils is 1, denote all soluble organic matter in the soluble sub-library of the representative soil as the soluble organic matter of the soil to be analyzed; when the number of representative soils is greater than 1, denote the soluble organic matter that exists in the soluble sub-libraries of all representative soils as the soluble organic matter of the soil to be analyzed.

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