Wearable Parkinson's disease treatment monitoring equipment and use method thereof

Abstract

The invention discloses wearable Parkinson's disease treatment monitoring equipment and a use method thereof. The wearable Parkinson's disease treatment monitoring equipment comprises a flexible patch, a flexible circuit board and a mobile intelligent terminal app, the flexible patch comprises a substrate layer, an electrode layer, a flow channel layer and a packaging layer, the electrode layer comprises electric stimulation induction double electrodes, levodopa, tryptophan and a pH sensing electrode, and a sweat stimulation area and a sensing area are separated under the assistance of the flow channel layer; the terminal and the clamping groove are used for connecting the patch and the flexible circuit board; the flexible circuit board excites the redox reaction of the electrode interface target through an ampere method and an open-circuit voltage method, acquires and reads an electric signal, and transmits data to a patient mobile intelligent terminal app through a Bluetooth module on the flexible circuit board. The device is attached to the body surface in the form of plaster, has high human body compliance, can realize real-time visualization of biochemical data of a patient, realizes a closed loop in the diagnosis and treatment process of Parkinson's disease, and promotes the modernization of medical treatment and care equipment.

Description

Technical Field

[0001] The present invention relates to the field of wearable rehabilitation treatment for Parkinson's disease, and particularly to a wearable Parkinson's disease treatment monitoring device and its usage method. Background Art

[0002] Parkinson's disease is a common neurodegenerative disease. Its main pathological change is the degeneration and death of dopamine neurons in the substantia nigra of the midbrain, resulting in a significant decrease in dopamine content in the striatum and causing the disease. Clinically, it is mainly manifested as resting tremor, bradykinesia, muscle rigidity, and postural gait disorders, and is accompanied by non-motor symptoms such as depression, constipation, and sleep disorders. Drug intake and diet regulation are of great significance for controlling the condition and alleviating symptoms. Levodopa is a long-term oral drug for Parkinson's disease, but its dosing window is narrow, and the drug efficacy shows a weakening trend in the later stage. Therefore, it is crucial to customize the intake dose and regimen. Tryptophan is one of the important raw materials for synthesizing neurotransmitters. The products of its kynurenine metabolic pathway are closely related to the pathogenesis of Parkinson's disease. Appropriate intake of tryptophan helps to improve the function of the nervous system. Therefore, during the rehabilitation treatment process of Parkinson's disease patients, real-time monitoring of the amino acid and drug content in the patient's body, and timely warning of drug intake and diet therapy are crucial for the treatment of patients and assisting doctors in formulating targeted diagnosis and treatment plans.

[0003] Currently, the non-invasive wearable devices under research for Parkinson's disease mainly focus on the mechanical monitoring of motor symptoms and electrotherapy stimulation devices, and there is a relative lack of monitoring for high-throughput drug molecules and biotin. The existing sensing devices are mainly centralized monitoring based in hospitals, and the devices have bottleneck problems such as huge volume, high price, inconvenient to carry, poor human compliance, and long detection time, seriously interfering with the quality of life of patients and being unfavorable for doctors to give reasonable drug administration and diet therapy plans.

[0004] Therefore, providing a wearable rehabilitation treatment monitoring device for Parkinson's disease that can be high-throughput and personalized, and can complete real-time monitoring of the necessary biochemical information of patients, and adjust the diagnosis and treatment plan according to the monitoring data to form a medical closed-loop device and method is the technical problem that the present invention urgently needs to solve. Summary of the Invention

[0005] In view of this, the present invention provides a wearable Parkinson's disease treatment monitoring device and its usage method.

[0006] To solve the above technical problems, the present invention adopts the following technical solutions:

[0007] A wearable Parkinson's disease treatment and monitoring device, comprising a flexible patch, a flexible circuit board and a mobile smart terminal app; the flexible patch includes a substrate layer, an electrode layer, a flow channel layer and a packaging layer, the electrode layer includes an electrostimulation-induced double electrode and levodopa, tryptophan and pH sensing electrodes, and divides the sweat stimulation area and the sensing area with the assistance of the flow channel layer; terminals and card slots are used to connect the patch and the flexible circuit board; the flexible circuit board stimulates the redox reaction of the electrode interface target by amperometry and open circuit voltage method, collects and reads the electrical signals, and transmits the data to the patient's mobile smart terminal app through the Bluetooth module on the flexible circuit board.

[0008] Through the above operations, the packaging layer makes the sensing process in a semi-open state to prevent the influence of potential interfering substances.

[0009] Preferably, the electrode layer is composed of a three-dimensional highly conductive material and is assembled to the substrate layer by means of dropping, curing and transferring.

[0010] Preferably, the electrostimulation-induced double electrode includes a cathode electrode and an anode electrode, which are located at both ends of the sweat stimulation area, i.e., the sensing area; the cathode electrode is composed of a three-dimensional highly conductive material and a hydrogel, the cathode electrode is composed of a three-dimensional highly conductive material and the hydrogel embedding a sweat gland stimulation drug, and the sweat gland stimulation drug includes any one of acetylcholine and pilocarpine.

[0011] Preferably, the levodopa, tryptophan and pH sensing electrodes are composed of two reference electrodes, three working electrodes and a counter electrode;

[0012] Among them, the two reference electrodes are reference electrode 1 and reference electrode 2 respectively; the three working electrodes are working electrode 1, working electrode 2 and working electrode 3 respectively;

[0013] The counter electrode and the reference electrode 1 wrap the two semi-circular sector-shaped working electrode 1 and working electrode 2, and the working electrode 3 and the reference electrode 2 are wrapped on the outermost layer of the sensing area; the working electrode 1 and the working electrode 2 are zinc oxide-functionalized three-dimensional highly conductive materials, which catalyze the gain and loss of electrons of levodopa and tryptophan to realize the conversion of biochemical signals into electrical signals, the working electrode 3 is a polyaniline-functionalized three-dimensional highly conductive material for sensitive pH monitoring, the reference electrode 1 is composed of silver and ferric chloride successively modifying the three-dimensional highly conductive material, and the reference electrode 2 is further modified with polyvinyl butyral on the basis of the reference electrode 1;

[0014] Among them, the modification method is any one of electrodeposition, chemical reduction and magnetron sputtering.

[0015] Preferably, the flexible circuit board (2) mainly consists of a microcontroller, a low-power Bluetooth module, a system power supply, a boost converter, a low-power voltage regulator, an electrochemical acquisition circuit, and a voltage-controlled constant current source; the electrochemical acquisition circuit has two detection methods for current-voltage and open-circuit voltage. Among them, the current-voltage detection circuit consists of a bias potential circuit, a low-pass filter, and a transimpedance amplifier; the open-circuit voltage detection circuit consists of a voltage follower and a low-pass filter; the i-t detection circuit in the electrochemical acquisition circuit applies a bias voltage generated by the analog-to-digital converter inside the microcontroller to the reference electrode and the counter electrode, and then collects the current generated by the redox reaction at the working electrode interface, converts it into a voltage signal through a transimpedance amplifier, and converts it into a digital signal through the analog-to-digital converter inside the microcontroller; the open-circuit voltage detection circuit in the electrochemical acquisition circuit detects the open-circuit voltage at the electrode end through a voltage follower composed of a high-input impedance operational amplifier, and after passing through a low-pass filter, it is converted into a digital signal through the analog-to-digital converter inside the microcontroller; and then it is transmitted to the mobile intelligent terminal app (3) through the low-power Bluetooth module for processing, analysis, and presentation.

[0016] Preferably, the mobile intelligent terminal app includes any one or several of an ipad, a smart phone, and a portable notebook.

[0017] Preferably, the specific steps for transferring the electrode layer to the substrate layer are as follows:

[0018] S1. Clean the carbon-based thin film substrate with acetone, ethanol, and ultrapure water in sequence and then dry it. Select carbon-based substrates containing nitrogen and sulfur elements, including polyimide (containing nitrogen), polyphenylene sulfide (containing sulfur), wood products, etc., not limited to one or more of them;

[0019] Through the above operations, nitrogen and sulfur elements can provide electron holes for the carbon skeleton to improve the conductivity of the material.

[0020] S2. Use a CO2 infrared laser engraving machine to carbonize the thin film on the surface of the substrate to obtain the electrode layer pattern, and design the electrode port arrangement to be consistent with the terminal spacing and size;

[0021] S3. Place the carbonized thin film in a mold, pour in a liquid elastomer, and through curing treatment, transfer the carbonized pattern to the substrate layer; styrene-ethylene / butene-styrene block copolymer, polydimethylsiloxane, polyvinylidene fluoride, polylactic acid, etc., not limited to one or more of them;

[0022] Among them, the curing treatment methods include any one or several of liquid nitrogen treatment, vacuum evaporation, and high-temperature curing.

[0023] Preferably, the specific steps for the zinc oxide-functionalized three-dimensional highly conductive material are as follows:

[0024] P1. Prepare an electrodeposition solution containing 0.1 M zinc nitrate solution and 0.05 M potassium chloride solution

[0025] P2. Immerse the working electrode 1 (11) and the working electrode 2 (12) in the electrodeposition solution, connect them to a platinum wire electrode to form a circuit, connect a reference electrode, and perform electrodeposition for 800 s at a voltage of -0.8 V.

[0026] Preferably, the preparation steps of the polyaniline-functionalized three-dimensional highly conductive material are as follows:

[0027] Q1. Prepare an electrodeposition solution containing aniline and hydrochloric acid solution;

[0028] Q2. Immerse the working electrode 3 in the electrodeposition solution, connect it to a platinum wire electrode to form a circuit, connect a reference electrode, and perform electrodeposition for 800 s at a voltage of -0.8 V.

[0029] A usage method of a wearable Parkinson's disease treatment and monitoring device includes the following steps:

[0030] T1. Turn on and regulate the mobile phone app for monitoring. The instructions are transmitted to the flexible circuit board through the Bluetooth module. Through the general input / output interface in the microprocessor, the computing tree logic adjusts the voltage to control the constant current circuit, and regulates the electrostimulation voltage applied across the anode and cathode electrodes, promoting the secretion of drugs in the hydrogel on the surface of the anode electrode into the sweat glands. After the sweat glands receive the drug information, a large amount of sweating occurs in the area between the anode and cathode electrodes;

[0031] T2. The sweat is collected through the microchannel to the sensing area. The target substances levodopa, tryptophan, and acid radicals respond to the voltage signal applied under the instruction of the microprocessor at the interfaces of the working electrode 1, the working electrode 2, and the working electrode 3. The interface current and open-circuit voltage intensity are collected. The collected electrical signals are amplified by a voltage amplifier and a transimpedance amplifier, and then transmitted back to the microprocessor. They are transmitted to the mobile intelligent terminal app through the low-power Bluetooth module. The signals are processed by software, and the concentrations of levodopa and tryptophan are monitored in real time according to the standard curve, and early warnings for drug intake and eating are given in a timely manner.

[0032] Through the above operations, T1 can cause a large amount of sweating after the sweat glands receive the drug information; T2 can improve the physical functions of the patient and control the development of the disease.

[0033] The present invention has achieved the following technical effects compared with the prior art:

[0034] (1) The present invention adopts a flexible patch and a flexible circuit board, which improves the compliance of the device with the human skin. In the form of a plaster, it is convenient to carry without feeling.

[0035] (2) The present invention can achieve the conversion between multi-parameter independent monitoring and simultaneous monitoring by adjusting the microprocessor, and the two are non-interfering with each other;

[0036] (3) Through the effective design of the preparation of the electrode material, the present invention designs a variety of composite porous scaffold materials, which meet the requirements of efficient electrocatalysis and electrical conduction, and have high flexibility and good adhesion, ensuring the sensitivity and stability of the device. Brief Description of the Drawings

[0037] Figure 1 Schematic diagram of a wearable rehabilitation treatment monitoring device for Parkinson's disease disclosed in an embodiment of the present invention;

[0038] Figure 2 Structural diagram of the flexible chip assembly disclosed in an embodiment of the present invention;

[0039] Figure 3 Schematic diagram of the electrode layer disclosed in an embodiment of the present invention;

[0040] Figure 4 Structural diagram of the flexible circuit board disclosed in an embodiment of the present invention;

[0041] Figure 5 Reaction of the working electrode interface disclosed in an embodiment of the present invention;

[0042] Figure 6 Scanning electron microscope pattern of the working electrode interface disclosed in an embodiment of the present invention;

[0043] Among them, (a) is a 10μm diagram; (b) is a 3μm diagram;

[0044] Figure 7 Relationship curve between tryptophan concentration and electrical signal disclosed in an embodiment of the present invention;

[0045] Figure 8 Relationship curve between levodopa concentration and electrical signal disclosed in an embodiment of the present invention;

[0046] Figure 9 Relationship curve between pH concentration and electrical signal disclosed in an embodiment of the present invention.

[0047] Among them, 1. Flexible patch; 2. Flexible circuit board; 3. Mobile intelligent terminal app; 4. Terminal; 5. Card slot; 6. Substrate layer; 7. Electrode layer; 8. Flow channel layer; 9. Encapsulation layer; 10. Hydrogel; 11. Working electrode 1; 12. Working electrode 2; 13. Working electrode 3; 14. Reference electrode 1; 15. Reference electrode 2; 16. Counter electrode; 17. Anode electrode; 18. Cathode electrode. Detailed Description of the Invention

[0048] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts belong to the scope of protection of the present invention.

[0049] The present invention discloses a wearable Parkinson's disease treatment and monitoring device, including a flexible patch 1, a flexible circuit board 2, a mobile intelligent terminal app 3, terminals 4, and a card slot 5; as Figure 1 shown; the flexible patch 1 includes a substrate layer 6, an electrode layer 7, a flow channel layer 8, and a packaging layer 9, as Figure 2 shown. The electrode layer 7 is composed of three-dimensional porous graphene and is assembled to the substrate layer 6 by means of dropping, curing, and transfer. The electrode layer 7 includes an electrostimulation-induced bipolar electrode and levodopa, tryptophan, and pH sensing electrodes. With the assistance of the flow channel layer 8, the sweat stimulation area and the sensing area are separated to induce, collect, and monitor the patient's sweat. The packaging layer 9 makes the sensing process in a semi-open state to prevent the influence of potential interfering substances; the substrate layer 6, the flow channel layer 9, and the packaging layer 9 are permanently bonded by oxygen plasma treatment; the terminals 4 and the card slot 5 are used to connect the patch and the flexible circuit board 2; the flexible circuit board 2 stimulates the redox reaction of the electrode interface target by amperometry and open circuit voltage method, collects and reads the electrical signal, and transmits the data to the patient's mobile intelligent terminal app 3 through the Bluetooth module on the flexible circuit board 2 for levodopa, tryptophan, and pH monitoring and timely warning of drug and diet therapy.

[0050] The electrostimulation-induced bipolar electrode includes a cathode electrode 18 and an anode electrode 17 located at both ends of the sweat stimulation area, that is, the sensing area; the cathode electrode 18 is composed of a three-dimensional highly conductive material and a hydrogel 10, and the cathode electrode 18 is composed of a three-dimensional highly conductive material and the hydrogel 10 embedding a sweat gland stimulating drug, and the sweat gland stimulating drug is acetylcholine.

[0051] The levodopa, tryptophan, and pH sensing electrodes are composed of two reference electrodes, three working electrodes, and one counter electrode;

[0052] The two reference electrodes are respectively a reference electrode 1 14 and a reference electrode 2 15; the three working electrodes are respectively a working electrode 1 11, a working electrode 2 12, and a working electrode 3 13; one counter electrode is a counter electrode 16;

[0053] The counter electrode 16 and the reference electrode 1 14 wrap two semi-circular sector-shaped working electrodes 1 11 and working electrode 2 12, and the working electrode 3 13 and the reference electrode 2 15 are wrapped on the outermost layer of the sensing area; the working electrode 1 11 and the working electrode 2 12 are zinc oxide-functionalized three-dimensional highly conductive materials that catalyze the gain and loss of electrons of levodopa and tryptophan to achieve the conversion of biochemical signals to electrical signals. The working electrode 3 13 is a polyaniline-functionalized three-dimensional highly conductive material for sensitive pH monitoring. The reference electrode 1 14 is composed of silver and ferric chloride successively modifying a three-dimensional highly conductive material, and the reference electrode 2 15 is further modified with polyvinyl butyral on the basis of the reference electrode 1 14. The electrode layout is as Figure 3 shown.

[0054] The modification method is any one of electrodeposition, chemical reduction, and magnetron sputtering.

[0055] The terminal 4 and the card slot 5 are conventional commercial connection components; the terminal 4 is a male terminal, front connecting band, and center rivet. The connection method is a sharp pin, and it is connected to the electrode layer of the flexible patch by riveting for electron conduction for collection.

[0056] The flexible circuit board 2 is mainly composed of a microcontroller, a low-power Bluetooth module, a system power supply, a boost converter, and a low-power voltage regulator, as Figure 4 shown; the microcontroller is composed of a digital-to-analog converter, an analog-to-digital converter, a general-purpose input / output interface, a low-pass filter, a voltage amplifier, and a transimpedance amplifier; the microcontroller transmits the excitation voltage to the electrode layer, then collects the electrons generated by the redox reaction at the electrode interface, performs amplification processing, and transmits them to a mobile intelligent terminal app through the low-power Bluetooth module for processing, analysis, and presentation;

[0057] The specific steps for transferring the electrode layer 7 to the substrate layer 6 are:

[0058] S1. Clean the carbon-based thin film substrate with acetone, ethanol, and ultrapure water in sequence and then dry it. It is optimal for the carbon-based substrate to contain nitrogen and sulfur elements, which can provide electron holes for the carbon skeleton to improve the conductivity of the material;

[0059] S2. Use a CO2 infrared laser engraving machine with a power of 11W and a scanning speed of 130mm / s. Under the parameter of a step size of 100 microns, carbonize the thin film on the substrate surface to obtain the electrode layer pattern, and design the electrode port arrangement to be consistent with the terminal spacing and size;

[0060] S3. Place the carbonized thin film in a mold, pour in a liquid elastomer, and through curing treatment, transfer the carbonized pattern to the substrate layer 6. The curing treatment methods include any one or several of liquid nitrogen treatment, vacuum evaporation, and high-temperature curing.

[0061] In the above solution, the preparation steps of the zinc oxide-functionalized three-dimensional highly conductive material are as follows:

[0062] P1. Prepare an electrodeposition solution containing 0.1 M zinc nitrate solution and 0.05 M potassium chloride solution

[0063] P2. Immerse the working electrode 1 11 and the working electrode 2 12 in the electrodeposition solution, connect them to a platinum wire electrode to form a circuit, connect a reference electrode, and perform electrodeposition at a voltage of -0.8 V for 800 s.

[0064] The preparation steps of the polyaniline-functionalized three-dimensional highly conductive material are as follows:

[0065] Q1. Prepare an electrodeposition solution containing aniline and hydrochloric acid solution;

[0066] Q2. Immerse the working electrode 3 13 in the electrodeposition solution, connect it to a platinum wire electrode to form a circuit, connect a reference electrode, and perform electrodeposition at a voltage of -0.8 V for 800 s.

[0067] The present invention also discloses a usage method of a wearable Parkinson's disease treatment and monitoring device, including the following steps:

[0068] T1. Turn on and regulate the mobile phone app for monitoring. The instruction is transmitted to the flexible circuit board (2) through the Bluetooth module. Through the general input / output interface in the microprocessor, the voltage control constant current circuit is turned on / off, and the electrical stimulation voltage applied across the anode and cathode electrodes (18) is regulated to promote the secretion of the drug in the hydrogel (10) on the surface of the anode electrode (17) into the sweat glands;

[0069] T2. The sweat is collected to the sensing area through the microchannel. The target substances levodopa, tryptophan, and acid radicals are subjected to a voltage signal applied by the microprocessor instruction at the interfaces of the working electrode 1 (11), the working electrode 2 (12), and the working electrode 3 (13), and the interfacial current response and open circuit voltage intensity are collected. The interfacial reaction is as Figure 5 shown and the interfacial scanning electron microscope picture is as Figure 6 shown. The collected electrical signal is amplified by a transimpedance amplifier and a voltage amplifier. The microprocessor collects this signal through the built-in analog-to-digital converter and transmits it to the mobile intelligent terminal app (3) through the low-power Bluetooth module. The signal is processed by software, and based on the standard curve as Figures 7-9 shown, the concentrations of levodopa and tryptophan are monitored in real time, and early warnings for drug intake and eating are given in a timely manner to improve the physical function of the patient and control the development of the disease.

[0070] As mentioned above, it is only the preferred embodiment of the present invention, and does not impose any limitation on the technical scope of the present invention. Therefore, any minor modification, equivalent change and modification made to the above embodiments based on the technical essence of the present invention still fall within the scope of the technical solution of the present invention.

Claims

1. A wearable Parkinson's disease treatment and monitoring device, characterized in that, It includes a flexible patch (1), a flexible circuit board (2) and a mobile smart terminal app (3); the flexible patch (1) comprises a substrate layer (6), an electrode layer (7), a flow channel layer (8) and a packaging layer (9), the electrode layer (7) comprises an electrostimulation-induced double electrode and levodopa, tryptophan and pH sensing electrodes, and separates a sweat stimulation area and a sensing area with the assistance of the flow channel layer (8); terminals (4) and card slots (5) are used to connect the patch and the flexible circuit board; the flexible circuit board (2) stimulates the redox reaction of the electrode interface target by amperometry and open circuit voltage method, collects and reads the electrical signal, and transmits the data to the patient's mobile smart terminal app (3) through the Bluetooth module on the flexible circuit board (2).

2. The wearable Parkinson's disease treatment monitoring device according to claim 1, wherein The electrode layer (7) is composed of a three-dimensional highly conductive material and is assembled to the substrate layer (6) by means of dropping, curing and transferring.

3. The wearable Parkinson's disease treatment and monitoring device according to claim 1, characterized in that, The electrostimulation-induced double electrode includes a cathode electrode (18) and an anode electrode (17), which are located at both ends of the sweat stimulation area, i.e., the sensing area; the cathode electrode (19) is composed of a three-dimensional highly conductive material and a hydrogel (10), and the cathode electrode (19) is composed of a three-dimensional highly conductive material and the hydrogel (10) embedded with a sweat gland stimulation drug, and the sweat gland stimulation drug includes any one of acetylcholine and pilocarpine.

4. The wearable Parkinson's disease treatment monitoring device according to claim 1, wherein, The levodopa, tryptophan and pH sensing electrodes are composed of two reference electrodes, three working electrodes and one counter electrode. Among them, the two reference electrodes are reference electrode 1 (14) and reference electrode 2 (15) respectively; the three working electrodes are working electrode 1 (11), working electrode 2 (12) and working electrode 3 (13) respectively; one counter electrode is counter electrode (16). The counter electrode (16) and the reference electrode 1 (14) wrap the two semi-circular sector-shaped working electrode 1 (11) and the working electrode 2 (12), and the working electrode 3 (13) and the reference electrode 2 (15) are wrapped on the outermost layer of the sensing area; the working electrode 1 (11) and the working electrode 2 (12) are zinc oxide-functionalized three-dimensional highly conductive materials, which catalyze the gain and loss of electrons of levodopa and tryptophan to realize the conversion of biochemical signals into electrical signals, the working electrode 3 (13) is a polyaniline-functionalized three-dimensional highly conductive material for sensitive monitoring of pH, the reference electrode 1 (14) is composed of a three-dimensional highly conductive material modified by silver and ferric chloride in sequence, and the reference electrode 2 (15) is further modified with polyvinyl butyral on the basis of the reference electrode 1 (14). Among them, the modification method is any one of electrodeposition method, chemical reduction and magnetron sputtering.

5. The wearable Parkinson's disease treatment and monitoring device according to claim 1, characterized in that, The flexible circuit board (2) mainly consists of a microcontroller, a low-power Bluetooth module, a system power supply, a boost converter, a low-power voltage regulator, an electrochemical acquisition circuit, and a voltage-controlled constant current source; the electrochemical acquisition circuit has two detection methods: current-voltage and open-circuit voltage. Among them, the current-voltage detection circuit consists of a bias potential circuit, a low-pass filter, and a transimpedance amplifier; the open-circuit voltage detection circuit consists of a voltage follower and a low-pass filter; the i-t detection circuit in the electrochemical acquisition circuit applies the bias voltage generated by the analog-to-digital converter inside the microcontroller to the reference electrode and the counter electrode, then collects the current generated by the redox reaction at the working electrode interface, converts it into a voltage signal through a transimpedance amplifier, and converts it into a digital signal through the analog-to-digital converter inside the microcontroller; the open-circuit voltage detection circuit in the electrochemical acquisition circuit detects the open-circuit voltage at the electrode end through a voltage follower composed of a high-input impedance operational amplifier, and after passing through a low-pass filter, it is converted into a digital signal through the analog-to-digital converter inside the microcontroller; then it is transmitted to the mobile intelligent terminal app (3) through the low-power Bluetooth module for processing, analysis, and presentation.

6. The wearable Parkinson's disease treatment monitoring device according to claim 1, characterized in that, The mobile intelligent terminal app (3) includes any one or several of an ipad, a smart phone, and a portable notebook.

7. The wearable Parkinson's disease treatment monitoring device according to claim 2, characterized in that, The specific steps for transferring the electrode layer (7) to the substrate layer (6) are as follows: S1. Clean the carbon-based thin film substrate with acetone, ethanol, and ultrapure water in sequence and then dry it, and select a carbon-based substrate containing nitrogen and sulfur elements; S2. Use a CO2 infrared laser engraving machine to carbonize the thin film on the substrate surface to obtain the electrode layer pattern, and design the electrode port arrangement to be consistent with the terminal interval and size; S3. Place the carbonized thin film in a mold, pour in a liquid elastomer, and through curing treatment, transfer the carbonized pattern to the substrate layer (6); Among them, the curing treatment method includes any one or several of liquid nitrogen treatment, vacuum evaporation, and high-temperature curing.

8. The wearable Parkinson's disease treatment and monitoring device according to claim 4, characterized in that, The specific steps for the zinc oxide-functionalized three-dimensional highly conductive material are as follows: P1. Prepare an electrodeposition solution, including a 0.1M zinc nitrate solution and a 0.05M potassium chloride solution P2. Immerse the working electrode 1 (11) and the working electrode 2 (12) in the electrodeposition solution, connect them to a platinum wire electrode to form a circuit, connect in a reference electrode, and perform electrodeposition at a voltage of -0.8V for 800s.

9. The wearable Parkinson's disease treatment monitoring device according to claim 1, wherein, The preparation steps for the polyaniline-functionalized three-dimensional highly conductive material are as follows: Q1. Prepare an electrodeposition solution, including aniline and hydrochloric acid solution; Q2. Immerse the working electrode 3 (13) in the electrodeposition solution, connect it to a platinum wire electrode to form a circuit, connect in a reference electrode, and perform electrodeposition at a voltage of -0.8V for 800s.

10. The usage method of a wearable Parkinson's disease treatment monitoring device according to claim 1, characterized in that, Include the following steps: T1. Turn on and regulate the mobile phone app for monitoring, and the command is transmitted to the flexible circuit board (2) through the Bluetooth module. Through the general-purpose input / output interface in the microprocessor, turn on / off the voltage control constant current circuit, and regulate the electric stimulation voltage applied across the anode and cathode electrodes (18) to promote the secretion of drugs in the hydrogel (10) on the surface of the anode electrode (17) into the sweat glands; T2. The sweat is collected through the microchannel to the sensing area. At the interfaces of the working electrode 1 (11), the working electrode 2 (12), and the working electrode 3 (13), the target substances, i.e., levodopa, tryptophan, and acid radical ions, are applied with a voltage signal under the instruction of the microprocessor to collect the interfacial current response and the open-circuit voltage intensity. The collected electrical signals are amplified by a transimpedance amplifier and a voltage amplifier. The microprocessor collects this signal through a built-in analog-to-digital converter and transmits it to the mobile intelligent terminal app (3) through a low-power Bluetooth module. The signal is processed by software, and according to the standard curve, the concentrations of levodopa and tryptophan are monitored in real time to give early warnings for drug intake and eating in a timely manner.

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