Vacuum device for fixed preservation of plant sample and preservation method thereof

Abstract

The invention discloses a plant sample fixed preservation vacuum device and a preservation method thereof, and relates to the technical field of plant sample preservation, the plant sample fixed preservation vacuum device comprises a vacuumizing device and a vacuum box used for placing a plurality of operation vessels, the side wall of the vacuum box is provided with a ventilation channel, and the ventilation channel is detachably connected with the vacuumizing device; according to the plant sample preservation device, the multiple operation vessels are placed in the vacuum box, synchronous vacuumizing of the multiple operation vessels can be achieved by starting the vacuumizing equipment, the plant sample preservation efficiency can be effectively improved, meanwhile, the vacuum degrees in the multiple operation vessels are consistent, and the vacuum degrees can be controlled through the vacuumizing equipment; the preservation effect of the plant sample is further improved; besides, the ventilation channel is formed in the side wall, so that gas is blown to the side wall of the operation vessel in the process of putting the gas into the vacuum box from the ventilation channel, and the problems that the gas directly blows the liquid level of the operation vessel from top to bottom, so that the stationary liquid is splashed, and then the stationary liquid is wasted and the vacuum box is polluted are solved.

Description

Technical Field

[0001] The present invention relates to the technical field of plant sample preservation, and particularly to a vacuum device for fixing and preserving plant samples and a preservation method thereof. Background Art

[0002] After plant biological samples are obtained in the wild, they usually need to be quickly put into prepared fixing solutions (such as FAA fixing solution, Carnoy's fixing solution, etc.) for fixing and preservation. Due to the presence of cell walls and the particularity of some plant structures, it is generally necessary to evacuate the air first to make the plant samples sink to the bottom, so that the fixing solution can penetrate into the plant tissues as soon as possible, preserve the original appearance of the target structure, and quickly discharge the air to facilitate subsequent replacement and embedding processes without leaving air bubbles and prevent various sections from being broken.

[0003] Currently, during the field sampling process, the method for fixing and preserving plant samples is as follows: Using a vial with a rubber stopper as the operating vessel, placing the fixing solution and plant samples in the vial, covering the rubber stopper, and then using a large-capacity syringe to simply aspirate the vial to achieve the operation of evacuating the air and sinking to the bottom. However, when taking multiple samples at one time during field sampling, when using the above method for fixing and preservation, it is necessary to evacuate the air in multiple vials one by one, resulting in low preservation efficiency, and the aspiration time and vacuum degree for each time cannot be quantified, and the preservation effect is poor.

[0004] Therefore, there is an urgent need for a vacuum device for fixing and preserving plant samples with high processing efficiency and good processing effect. Summary of the Invention

[0005] The purpose of the present invention is to provide a vacuum device for fixing and preserving plant samples and a preservation method thereof to solve the problems existing in the above-mentioned prior art, which can evacuate the air in multiple operating vessels synchronously, improve the preservation efficiency, and at the same time, the vacuum degrees of multiple operating vessels are consistent, improving the preservation effect.

[0006] To achieve the above purpose, the present invention provides the following solution: The present invention provides a vacuum device for fixing and preserving plant samples, including a vacuum pumping device and a vacuum chamber for placing multiple operating vessels. An air vent passage is provided on the side wall of the vacuum chamber, the air vent passage is communicated with the inner cavity of the vacuum chamber, and the air vent passage is detachably connected to the vacuum pumping device.

[0007] Preferably, the air vent passage is communicated with the vacuum pumping device through a hose.

[0008] Preferably, a flared ring is provided on the outer peripheral wall of the air vent passage, the outer diameter of the flared ring is larger than the inner diameter of the hose, and the hose is press-fitted with the flared ring.

[0009] Preferably, an air treatment interlayer is provided in the vacuum chamber. The air treatment interlayer includes an interlayer plate, and accommodation grooves for placing granular air treatment agents are formed in the interlayer plate. The accommodation grooves are open at the top, and ventilation holes are formed in opposite side walls of the accommodation grooves. The side walls of the accommodation grooves provided with the ventilation holes are closely attached to the inner side walls of the ventilation channels communicating with the vacuum chamber.

[0010] Preferably, the vacuum chamber includes a box body and a sealing cover. The sealing cover is buckled at the top opening of the box body, and a sealing ring is provided between the sealing cover and the box body.

[0011] Preferably, the sharp corners between adjacent side walls of the box body and the sealing cover are rounded.

[0012] Preferably, the operating vessels are vials and centrifuge tubes. A first placement rack for placing vials and a second placement rack for placing centrifuge tubes are provided in the vacuum chamber.

[0013] Preferably, the first placement rack includes a bottom plate, long side plates and short side plates. The long side plates and the short side plates are alternately arranged around the bottom plate. The long side plates are in a wavy shape matching the outer peripheral wall of the vial, and the wave crests and wave troughs of the two long side plates are arranged opposite to each other.

[0014] Preferably, the second placement rack is placed in the space enclosed by the long side plates and the short side plates. The second placement rack includes a perforated plate and a support plate. The support plate is arranged at the bottom of the perforated plate, and placement holes matching the centrifuge tubes are formed in the perforated plate.

[0015] The present invention also provides a preservation method for fixing and preserving plant samples by applying the vacuum device for fixing and preserving plant samples as described above, including the following steps: S1: Take a plant sample and place it in an operating vessel containing a fixing solution; S2: Place the operating vessel in the vacuum chamber, start the vacuum pumping device, and pump the vacuum chamber; S3: After the plant sample sinks to the bottom, turn off the vacuum pumping device, open the vacuum chamber, and replace the operating vessel and fill it with a new fixing solution; S4: Seal the operating vessel with a cover body and seal it with a sealing film.

[0016] The present invention mainly achieves the following technical effects compared with the prior art: Place multiple operating vessels in the vacuum chamber and start the vacuum pumping equipment to achieve synchronous vacuum pumping of multiple operating vessels, which can effectively improve the preservation efficiency of plant samples. At the same time, the vacuum degrees in multiple operating vessels are consistent, and the vacuum degree can be controlled by the vacuum pumping equipment, thereby improving the preservation effect of plant samples. In addition, an air vent channel is provided on the side wall, so that when gas is introduced into the vacuum chamber from the air vent channel, the gas blows against the side wall of the operating vessel, avoiding the problem that the gas blows directly onto the liquid surface of the operating vessel from top to bottom, resulting in splashing of the fixing liquid, and thus wasting the fixing liquid and polluting the vacuum chamber.

[0017] The following technical effects are achieved by other solutions of the present invention compared with the prior art: The setting of the flared ring can improve the plugging and sealing effect with the hose on the basis of realizing detachable connection with the hose.

[0018] The air treatment interlayer can treat the gas introduced from the air vent channel to avoid polluting the vacuum chamber and the operating vessels. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for use in the embodiments. Obviously, the drawings in the following description are only some embodiments of the present invention. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative efforts.

[0020] Figure 1 It is a schematic structural diagram of a vacuum device for fixing and preserving plant samples in an embodiment of the present invention; Figure 2 For Figure 1 The enlarged structural view of part A in Figure 3 It is a top view of the internal structure of the box body in an embodiment of the present invention; Figure 4 It is a schematic structural diagram of the internal structure of the box body in an embodiment of the present invention; Figure 5 It is a schematic structural diagram of the sandwich panel in an embodiment of the present invention; Figure 6 It is a schematic structural diagram of the first placement rack in an embodiment of the present invention; Figure 7 It is a schematic structural diagram of the second placement rack in an embodiment of the present invention; Figure 8 It is a schematic structural diagram of the vacuum chamber when an air release channel is provided in an embodiment of the present invention; Among them, 1. Vacuum chamber; 2. Ventilation channel; 3. Flared ring; 4. Sandwich panel; 5. Accommodating groove; 6. Ventilation holes; 7. Box body; 8. Sealing cover; 9. First placement rack; 10. Second placement rack; 11. Bottom plate; 12. Long side plate; 13. Short side plate; 14. Perforated plate; 15. Support plate; 16. Placement holes; 17. Handle; 18. Air release channel; 19. Rotating cap; 20. Air release holes. Detailed implementation mode

[0021] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0022] The purpose of the present invention is to provide a vacuum device for fixing and preserving plant samples and its preservation method to solve the problems existing in the prior art. It can synchronously evacuate multiple operating vessels, improve the preservation efficiency, and at the same time, the vacuum degrees of the multiple operating vessels are consistent, improving the preservation effect.

[0023] To make the above objects, features, and advantages of the present invention more obvious and understandable, the present invention will be further described in detail below with reference to the accompanying drawings and specific implementation modes.

[0024] Please refer to as Figures 1 to 8 As shown in the figure, a vacuum device for fixing and preserving plant samples is provided, including a vacuum extraction device and a vacuum chamber 1 for placing multiple operating vessels. The vacuum extraction device can be a rechargeable air pump, which does not need to be plugged in when used in the wild, improving its portability. In addition, in the case of a vehicle-mounted power supply or other power supply, a plug-in air pump or vacuum pump can also be selected, as long as it can extract air; a ventilation channel 2 is provided on the side wall of the vacuum chamber 1, and the ventilation channel 2 is communicated with the inner cavity of the vacuum chamber 1. The ventilation channel 2 is detachably connected to the vacuum extraction device. By placing multiple operating vessels in the vacuum chamber 1 and starting the vacuum extraction device, synchronous vacuum extraction of multiple operating vessels can be achieved, effectively improving the preservation efficiency of plant samples. At the same time, the vacuum degrees in multiple operating vessels are consistent, and the vacuum degree can be controlled by the vacuum extraction device, thereby improving the preservation effect of plant samples; in addition, by providing the ventilation channel 2 on the side wall, when gas is introduced into the vacuum chamber 1 from the ventilation channel 2, the gas blows against the side wall of the operating vessel, avoiding the problem that the gas blows directly down onto the liquid surface of the operating vessel, causing splashing of the fixing liquid, and thus wasting the fixing liquid and polluting the vacuum chamber 1.

[0025] The ventilation channel 2 is communicated with the vacuum extraction device through a hose, and the bendable characteristic of the hose is utilized to adapt to more field environments.

[0026] The outer peripheral wall of the ventilation passage 2 is provided with a flared ring 3. One or more flared rings 3 can be provided. The outer diameter of the flared ring 3 is larger than the inner diameter of the hose. The hose is press-fitted into the flared ring 3. On the basis of realizing the detachable connection with the hose, the plugging and sealing effect with the hose is improved.

[0027] The outer peripheral wall of the flared ring 3 is arc-shaped, that is, in the direction of approaching or departing from the end of the ventilation passage 2, the outer diameter of the flared ring 3 gradually decreases from the middle to the end, providing a guiding for the expansion during the insertion of the hose and improving the convenience of plugging.

[0028] The ventilation passage 2 can be designed as a frustum of a cone as a whole. The outer diameter of the end far from the vacuum chamber 1 is smaller than the outer diameter of the end close to the vacuum chamber 1, and the outer diameter of the end far from the vacuum chamber 1 is smaller than the inner diameter of the hose, further improving the convenience of plugging.

[0029] An air treatment interlayer is provided in the vacuum chamber 1. The air treatment interlayer includes an interlayer plate 4. A receiving groove 5 for placing granular air treatment agent is opened on the interlayer plate 4. The air treatment agent can be a desiccant or an acid-base neutralizing agent. The top of the receiving groove 5 is open. A plurality of air-permeable holes 6 are opened on the opposite side walls of the receiving groove 5. The air-permeable holes 6 can be strip-shaped, circular, rectangular, etc. The size of the air-permeable holes 6 is designed to ensure that the internal air treatment agent will not be discharged from the air-permeable holes 6. The side wall of the receiving groove 5 provided with the air-permeable holes 6 is closely attached to the inner side wall of the ventilation passage 2 communicating with the vacuum chamber 1. The gas introduced into the vacuum chamber 1 from the ventilation passage 2 first passes through the air treatment agent and then enters the vacuum chamber 1. The air treatment agent treats the gas introduced from the ventilation passage 2 to avoid polluting the vacuum chamber 1 and the operating vessels.

[0030] Two or more layers of interlayer plates 4 can be provided. The adjacent interlayer plates 4 are closely attached to each other. Different air treatment agents are placed in different interlayer plates 4 to improve the air treatment effect.

[0031] The interlayer plate 4 can be placed in the vacuum chamber 1 for easy removal and replacement of the air treatment agent, or can be integrally provided in the vacuum chamber 1. When the air treatment agent needs to be replaced, the whole vacuum chamber 1 needs to be turned over to pour out the air treatment agent in the interlayer plate 4.

[0032] When the interlayer plate 4 is placed in the vacuum chamber 1, legs can be provided at both ends of the bottom of the interlayer plate 4, and a groove body matching the legs is provided on the inner bottom wall of the vacuum chamber 1, so that the legs can be embedded in the groove body to realize the positioning of the interlayer plate 4.

[0033] In addition to deflating by using the venting channel 2, an additional deflating channel 18 can be provided. The deflating channel 18 is arranged on the side wall of the vacuum chamber 1 where the venting channel 2 is provided, and the deflating channel 18 corresponds to the accommodating groove 5. One end of the deflating channel 18 away from the vacuum chamber 1 is sealed. Venting through holes are formed on the peripheral wall of the deflating channel 18, and a rotating cap 19 is provided at one end of the deflating channel 18 away from the vacuum chamber 1. The rotating cap 19 can be rotatably buckled at one end of the deflating channel 18 away from the vacuum chamber 1; a vent hole 20 is formed on the rotating cap 19, and the venting through hole is located on the rotation path of the vent hole 20; by controlling the rotating cap 19, it can be controlled whether the vent hole 20 corresponds to the venting through hole. When the vent hole 20 corresponds to the venting through hole, gas can be introduced into the vacuum chamber 1. When the vent hole 20 does not correspond to the venting through hole, the venting through hole is closed and external gas cannot enter the vacuum chamber 1; when deflating by using the deflating channel 18, the rotating cap 19 can also be controlled to make the vent hole 20 partially correspond to or directly face the venting through hole to regulate the deflating speed.

[0034] The material of the rotating cap 19 can be a flexible material such as rubber or silica gel. The inner diameter of the rotating cap 19 is slightly smaller than the outer diameter of the deflating channel 18 to improve the sealing effect; anti-slip protrusions can be provided on the outer peripheral wall of the rotating cap 19 to facilitate applying force to control its rotation.

[0035] The vacuum chamber 1 includes a box body 7 and a sealing cover 8. The sealing cover 8 is buckled at the top opening of the box body 7. The sealing cover 8 can be hinged to the box body 7 through a hinge, or can be snap-connected to the box body 7, or can be directly buckled on the box body 7. When the two are hinged, a lock can also be provided on the box body 7 and the sealing cover 8; a sealing ring is provided between the sealing cover 8 and the box body 7 to improve the sealing effect between the sealing cover 8 and the box body 7.

[0036] The sharp corners between the adjacent side walls of the box body 7 and the sealing cover 8 are rounded to improve the use safety.

[0037] Common operating vessels are vials and centrifuge tubes. In this embodiment, a first placement rack 9 for placing vials and a second placement rack 10 for placing centrifuge tubes are provided in the vacuum chamber 1 to facilitate supporting and positioning the vials and centrifuge tubes during the vacuum pumping process.

[0038] In this embodiment, the first placement rack 9 includes a bottom plate 11, long side plates 12 and short side plates 13. The long side plates 12 and short side plates 13 are alternately arranged around the bottom plate 11. The long side plates 12 are in a wavy shape matching the outer peripheral wall of the vial. The wave crests and wave troughs of the two long side plates 12 are arranged opposite to each other, and two vials can be arranged between two wave troughs.

[0039] In this embodiment, the design of the first placement rack 9 is utilized to place the second placement rack 10 in the space surrounded by the long side plate 12 and the short side plate 13 to save space. Specifically, the second placement rack 10 includes a perforated plate 14 and a support plate 15. The support plate 15 is provided at the bottom of the perforated plate 14. The perforated plate 14 is provided with a placement hole 16 matching the centrifuge tube. In this embodiment, four support plates 15 are respectively arranged at the bottom of the four edges of the perforated plate 14. The four support plates 15 and the perforated plate 14 form an inverted trough structure.

[0040] When the second rack 10 is placed in the space surrounded by the long side plate 12 and the short side plate 13, the horizontal height of the orifice plate 14 of the second rack 10 is higher than the upper surface of the long side plate 12 and the short side plate 13, making it easy to take out the second rack 10.

[0041] A handle 17 may be provided on the first placement rack 9 to facilitate lifting the first placement rack 9 from the box body 7 of the vacuum box 1 .

[0042] A flexible pad, such as a flexible sponge pad or a flexible silicone pad, can be placed in the vacuum box 1. When a vial or centrifuge tube is placed in the vacuum box 1 and the vacuum box 1 needs to be transported, the flexible pad is placed between the sealing cover 8 and the vial or centrifuge tube. One end of the flexible pad contacts the sealing cover 8, and the other end contacts the vial or centrifuge tube. The flexible pad can limit the position of the vial or centrifuge tube, and prevent the vial or centrifuge tube from being damaged by shaking during transportation.

[0043] The present invention also provides a method for preserving plant samples using the vacuum device, comprising the following steps: S1: Take the plant sample and place it in a working vessel (select a vial or centrifuge tube) containing the fixative solution; S2: placing the operating vessel on the first placement rack 9 or the second placement rack 10 in the vacuum box 1, covering it with the sealing cover 8 to seal it, and starting the vacuum pumping device to evacuate the vacuum box 1; S3: After the plant sample sinks to the bottom, the vacuum device is turned off, the connection between the ventilation channel 2 and the vacuum device is disconnected (i.e., the hose is unplugged from the ventilation channel 2), the vacuum box 1 is opened, and the operating vessel is replaced and filled with new fixative. This avoids the problem of loss of volatile components of the old fixative due to vacuuming, which reduces the concentration and reduces the fixation and preservation effect; S4: Use the lid of the operating vessel to close the operating vessel, and use a sealing film to seal the operating vessel.

[0044] After step S4 is completed, if it is necessary to vacuum and fix it again, the operating vessel needs to be taken out of the vacuum box 1 and placed in a designated collection box; if it is not necessary to vacuum and fix it again, the operating vessel can be left in the vacuum box 1 for transportation, or taken out and placed in a designated collection box.

[0045] Adaptability changes made according to actual requirements are all within the protection scope of the present invention.

[0046] It should be noted that for those skilled in the art, it is obvious that the present invention is not limited to the details of the above-mentioned exemplary embodiments, and without departing from the spirit or basic characteristics of the present invention, the present invention can be implemented in other specific forms. Therefore, from any point of view, the embodiments should be regarded as exemplary and non-limiting. The scope of the present invention is defined by the appended claims rather than the above description. Therefore, all changes falling within the meaning and scope of the equivalent elements of the claims are intended to be embraced within the present invention. Any reference signs in the claims should not be construed as limiting the claimed rights.

[0047] Specific examples are used in the present invention to elaborate on the principles and implementation manners of the present invention. The description of the above embodiments is only used to help understand the method and its core idea of the present invention; at the same time, for those of ordinary skill in the art, according to the idea of the present invention, there will be changes in the specific implementation manners and application scopes. In summary, the content of this specification should not be construed as a limitation to the present invention.

Claims

1. A vacuum device for fixing and preserving plant samples, characterized in that, It includes a vacuum pumping device and a vacuum chamber for placing a plurality of operating vessels. An air vent passage is provided on the side wall of the vacuum chamber. The air vent passage communicates with the inner cavity of the vacuum chamber, and the air vent passage is detachably connected to the vacuum pumping device.

2. The vacuum device for fixing and preserving plant samples according to claim 1, wherein, The air vent passage communicates with the vacuum pumping device through a hose.

3. The vacuum device for fixing and preserving plant samples according to claim 2, characterized in that, An flared ring is provided on the outer peripheral wall of the air vent passage. The outer diameter of the flared ring is larger than the inner diameter of the hose, and the hose is press-fitted into the flared ring.

4. The vacuum device for fixing and preserving plant samples according to claim 1, characterized in that, An air treatment interlayer is provided in the vacuum chamber. The air treatment interlayer includes an interlayer plate. A receiving groove for placing granular air treatment agent is formed on the interlayer plate. The receiving groove is open at the top. Vent holes are formed on the opposite side walls of the receiving groove. The side wall of the receiving groove provided with the vent holes is closely attached to the inner side wall of the air vent passage communicating with the vacuum chamber.

5. The vacuum device for fixing and preserving plant samples according to claim 1, characterized in that, The vacuum chamber includes a box body and a sealing cover. The sealing cover is buckled on the top opening of the box body, and a sealing ring is provided between the sealing cover and the box body.

6. The vacuum device for fixing and preserving plant samples according to claim 5, characterized in that, The sharp corners between the adjacent side walls of the box body and the sealing cover are rounded.

7. The vacuum device for fixing and preserving plant samples according to claim 1, wherein The operating vessels are vials and centrifuge tubes. A first placement rack for placing vials and a second placement rack for placing centrifuge tubes are provided in the vacuum chamber.

8. The vacuum device for fixed preservation of plant samples according to claim 7, characterized in that, The first placement rack includes a bottom plate, long side plates and short side plates. The long side plates and the short side plates are alternately arranged around the bottom plate. The long side plates are in a wavy shape matching the outer peripheral wall of the vial, and the wave crests and wave troughs of the two long side plates are arranged opposite to each other.

9. The vacuum device for fixing and preserving plant samples according to claim 8, characterized in that, The second placement rack is placed in the space surrounded by the long side plates and the short side plates. The second placement rack includes a perforated plate and a support plate. The support plate is provided at the bottom of the perforated plate, and placement holes matching the centrifuge tubes are formed on the perforated plate.

10. A preservation method for fixing and preserving plant samples, characterized in that, Applying the vacuum device for fixing and preserving plant samples according to any one of claims 1-9, includes the following steps: S1: Take a plant sample and place it in an operating vessel containing a fixing solution; S2: Place the operating vessel in the vacuum chamber, start the vacuum pumping device, and evacuate the vacuum chamber; S3: After the plant sample sinks to the bottom, turn off the vacuum pumping device, open the vacuum chamber, and replace and fill the operating vessel with a new fixing solution; S4: Use a cover body to seal the operating vessel and seal it with a sealing film.

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