Execution sleeve automatic separation type nucleic acid extraction and purification instrument

Abstract

The invention discloses an execution sleeve automatic separation type nucleic acid extraction and purification instrument. The instrument comprises a stirring adsorption magnetic bead execution device, a mounting frame, a linear driving device and a kit, a magnetic bead stirring and adsorbing executing device is mounted on one side of the mounting frame, a linear driving device is mounted below the magnetic bead stirring and adsorbing executing device, a kit is detachably mounted at the output end of the linear driving device, and the linear driving device is used for driving the kit to be aligned with the executing end of the magnetic bead stirring and adsorbing executing device. According to the execution sleeve automatic separation type nucleic acid extraction and purification instrument, the problem that the whole nucleic acid extraction instrument is large in size due to the fact that a loading disc needs to be rotated to switch different kits to be aligned with a magnetic rod in the prior art is solved.

Description

Technical Field

[0001] The present invention relates to a biological detection device, and more particularly to an execution sleeve automatic detachment type nucleic acid extraction and purification instrument. Background Art

[0002] In the field of nucleic acid detection, nucleic acid extraction is a prerequisite for nucleic acid detection. The execution sleeve automatic detachment type nucleic acid extraction and purification instrument in this application only performs nucleic acid purification.

[0003] The principle of nucleic acid extraction is as follows: Reagents need to be added to magnetic beads to adsorb nucleic acids. After the magnetic beads are adsorbed, the nucleic acids need to be transferred by adsorbing the magnetic beads. Then, the magnetic beads are washed to adsorb the nucleic acids, so as to achieve the purpose of purification. Therefore, an execution sleeve automatic detachment type nucleic acid extraction and purification instrument is needed to transfer the magnetic beads and wash the magnetic beads to purify the nucleic acids.

[0004] As Figure 5 shown in the nucleic acid extraction steps by the magnetic bead method, the execution sleeve automatic detachment type nucleic acid extraction and purification instrument in this application only performs the purpose of obtaining purified nucleic acids after the binding, washing, and elution steps in the nucleic acid extraction process. In the binding step, it is stored as the sample lysis solution after adding magnetic beads; in the washing step, it is stored as the washing solution, which is used to wash away impurities outside the nucleic acids easily; in the elution step, it is stored as the elution solution, which is used to elute the nucleic acids from the magnetic beads to obtain purified nucleic acids.

[0005] Chinese Patent discloses a nucleic acid extractor with the application number: CN202120571467.7. This nucleic acid extractor includes a base, a driving motor is fixedly connected to the top of the base, an output end of the driving motor is fixedly connected to a loading disk, a plurality of grooves are formed in the loading disk, a reagent kit is inserted into each groove, each reagent kit includes a plurality of test tubes for storing reagents, a fixing frame is fixedly connected to the base, and a stirring mechanism is arranged on the fixing frame. The stirring mechanism includes a cylinder fixedly connected to the top of the base, and a mounting plate is arranged above the loading disk.

[0006] Although this nucleic acid extractor can solve the problem that the reagent remaining on the magnetic rod sleeve may fall into the adjacent test tube of the corresponding test tube when the turntable rotates in the prior art, the disadvantages of this nucleic acid extractor are still: Since the loading disk needs to rotate to switch different reagent kits to align with the magnetic rod, the volume of the entire nucleic acid extractor is large, increasing the cost of the nucleic acid extractor. Summary of the Invention

[0007] The present invention aims to provide an execution sleeve automatic detachment type nucleic acid extraction and purification instrument to solve the problem that the volume of the entire nucleic acid extractor is large due to the need to rotate the loading disk to switch different reagent kits to align with the magnetic rod in the prior art.

[0008] To achieve the above object, the present invention adopts the following technical solutions:

[0009] The present invention discloses an automatic detachment type nucleic acid extraction and purification instrument for an execution sleeve, comprising: a stirring and adsorbing magnetic bead execution device, a mounting frame, a linear driving device, and a reagent kit; a stirring and adsorbing magnetic bead execution device is installed on one side of the mounting frame, a linear driving device is installed below the stirring and adsorbing magnetic bead execution device, the output end of the linear driving device is detachably installed with the reagent kit, and the linear driving device is used to drive the reagent kit to align with the execution end of the stirring and adsorbing magnetic bead execution device. The stirring and adsorbing magnetic bead execution device comprises: an execution sleeve, a rotating shaft, a rotation driving mechanism, a first lifting seat, a first lifting driving mechanism, a magnetic rod, a second lifting seat, and a second lifting driving mechanism; the reagent kit is provided with a reagent groove, the reagent groove is for the execution sleeve to extend into, the reagent groove is used for storing reagents and magnetic beads, after the rotating shaft is inserted into the execution sleeve, it can be connected to the execution sleeve, after the rotating shaft extends into the execution sleeve, it can drive the execution sleeve to lift, the rotating shaft is rotatably installed on the first lifting seat, a rotation driving mechanism is installed on the first lifting seat, the rotation driving mechanism is used to drive the rotating shaft and the execution sleeve to rotate, the first lifting seat can lift relative to the mounting frame, a first lifting driving mechanism is installed on the mounting frame, and the output end of the first lifting driving mechanism is connected to the first lifting seat; a second lifting seat capable of lifting is installed on the mounting frame, the second lifting seat is fixed to the output end of the second lifting driving mechanism, the second lifting driving mechanism is installed on the first lifting seat or the mounting frame, a magnetic rod is installed on the second lifting seat, the magnetic rod can extend into the execution sleeve, and the second lifting driving mechanism can drive the magnetic rod to impact the execution sleeve to disengage from the rotating shaft.

[0010] Preferably, the execution sleeve comprises: a sleeve body and a buckle arm, the sleeve body extends into the reagent groove, at least two buckle arms extend from the top of the sleeve body, there is a gap between adjacent two buckle arms, and the buckle arm can be in buckle fit with the rotating shaft.

[0011] Preferably, an annular groove is provided on the outer wall of the rotating shaft, the buckle arm protrudes to form a protrusion, and the protrusion is in buckle fit with the inner wall of the annular groove.

[0012] Preferably, the rotating shaft comprises: a first shaft sleeve and a second shaft sleeve, the first shaft sleeve is rotatably installed on the first lifting seat, the first shaft sleeve is used to connect to the rotation driving mechanism, the bottom end of the first shaft sleeve is connected with the second shaft sleeve, and an annular groove is provided on the second shaft sleeve.

[0013] Preferably, a ball bearing is installed between the first shaft sleeve and the lifting seat.

[0014] Preferably, the stirring and adsorbing magnetic bead execution device, the mounting frame, the linear driving device, and the reagent kit are all installed in a box body, a window is provided on one side of the box body, and the window is opened and closed by a window door.

[0015] Compared with the prior art, the present invention has the following beneficial effects:

[0016] 1) In this application, the kit is placed directly below the stirring and adsorbing magnetic bead execution device, no longer occupying additional area outside the stirring and adsorbing magnetic bead execution device, avoiding the need for a loading tray for driving the rotation of different kits in the prior art, and avoiding the area occupied by the loading tray outside the stirring and adsorbing magnetic bead execution device. As a result, the overall execution sleeve automatic detachment nucleic acid extraction and purification instrument has a smaller volume, is more flexible and convenient to use, and also reduces costs.

[0017] 2) After the rotating shaft is inserted into the execution sleeve, it can be connected to the execution sleeve. The second lifting drive mechanism drives the magnetic rod to impact the execution sleeve and can be separated from the rotating shaft. Moreover, with the separated design of the rotating shaft and the magnetic rod, the rotating shaft can be driven to lift and lower by the first lifting drive mechanism, and the magnetic rod can be driven to lift and lower separately by the second lifting drive mechanism. By driving the execution sleeve to stir and mix, the execution sleeve can also be completely detached from the rotating shaft.

[0018] Other advantages, objectives, and features of the present invention will be partially reflected by the following description and partially understood by those skilled in the art through the research and practice of the present invention. Brief Description of the Drawings

[0019] Figure 1 It is a schematic structural diagram of an execution sleeve automatic detachment nucleic acid extraction and purification instrument.

[0020] Figure 2 It is a schematic structural diagram inside the execution sleeve automatic detachment nucleic acid extraction and purification instrument.

[0021] Figure 3 It is a cross-sectional view of the execution sleeve automatic detachment nucleic acid extraction and purification instrument.

[0022] Figure 4 For Figure 3 The enlarged view at position A in

[0023] Figure 5 It is the nucleic acid extraction steps by magnetic bead method.

[0024] Reference numerals: Stirring and adsorbing magnetic bead execution device 1, execution sleeve 11, sleeve body 111, buckle arm 112, protrusion 113, rotating shaft 12, annular groove 120, rotation drive mechanism 13, first lifting seat 14, first lifting drive mechanism 15, magnetic rod 16, second lifting seat 17, second lifting drive mechanism 18, mounting bracket 2, linear drive device 3, kit 4, reagent tank 40, box body 5, window door 50. Detailed Description of the Embodiment

[0025] In order to make the technical means, creative features, achieved objectives, and functions of the present invention clearer and easier to understand, the present invention will be further described below in conjunction with the drawings and specific embodiments:

[0026] As shown in Figures 1 to 4 the figure, the present invention discloses an automatic detachable nucleic acid extraction and purification instrument for an execution sleeve, comprising: a stirring and adsorbing magnetic bead execution device 1, a mounting frame 2, a linear driving device 3, and a reagent kit 4; the stirring and adsorbing magnetic bead execution device 1 is mounted on one side of the mounting frame 2, a linear driving device 3 is mounted below the stirring and adsorbing magnetic bead execution device 1, the output end of the linear driving device 3 is detachably mounted with the reagent kit 4, and the linear driving device 3 is used to drive the reagent kit 4 to align with the execution end of the stirring and adsorbing magnetic bead execution device 1.

[0027] The stirring and adsorbing magnetic bead execution device 1 comprises: an execution sleeve 11, a rotating shaft 12, a rotation driving mechanism 13, a first lifting seat 14, a first lifting driving mechanism 15, a magnetic rod 16, a second lifting seat 17, and a second lifting driving mechanism 18; the reagent kit 4 is provided with a reagent tank 40 for the execution sleeve 11 to extend into, and the reagent tank 40 is used to store reagents and magnetic beads. After the rotating shaft 12 is inserted into the execution sleeve 11, it can be connected to the execution sleeve, and after the rotating shaft 12 extends into the execution sleeve 11, it can drive the execution sleeve 11 to lift. The rotating shaft 12 is rotatably mounted on the first lifting seat 14, and the rotation driving mechanism 13 is mounted on the first lifting seat 14. The rotation driving mechanism 13 is used to drive the rotating shaft 12 and the execution sleeve 11 to rotate. The first lifting seat 14 can lift relative to the mounting frame 2, and the first lifting driving mechanism 15 is mounted on the mounting frame 2, and the output end of the first lifting driving mechanism 15 is connected to the first lifting seat 14; a second lifting seat 17 capable of lifting is mounted on the mounting frame 2, the second lifting seat 17 is fixed to the output end of the second lifting driving mechanism 18, and the second lifting driving mechanism 18 is mounted on the first lifting seat 14 or the mounting frame 2. A magnetic rod 16 is mounted on the second lifting seat 17, and the magnetic rod 16 can extend into the execution sleeve 11, and the second lifting driving mechanism can drive the magnetic rod to impact the execution sleeve to be separated from the rotating shaft.

[0028] Since the following operations need to be carried out to achieve the purpose of purifying nucleic acid: As shown in Figure 5 the magnetic bead method nucleic acid extraction steps, in the present application, the automatic detachable nucleic acid extraction and purification instrument for the execution sleeve only achieves the purpose of obtaining purified nucleic acid after the binding, washing, and elution steps in the nucleic acid extraction process. In the binding step, the sample lysis solution after adding magnetic beads is stored; in the washing step, the washing solution is stored, and the washing solution is used to easily wash away impurities outside the nucleic acid; in the elution step, the elution solution is stored, and the elution solution is used to elute the nucleic acid from the magnetic beads to obtain purified nucleic acid. One row of reagent tanks 40 is required for binding, one row of reagent tanks 40 is required for washing, one row of reagent tanks 40 is required for elution, and the last row of reagent tanks 40 stores the used magnetic beads and the execution sleeve 40 (similar to the "magnetic bead method nucleic acid extraction" in Baidu Encyclopedia). At least 4 rows of reagent tanks 40 are required for one purification.

[0029] In the combination step, the reagent tank 40 pre-stores the template lysis solution with magnetic beads. The template lysis solution contains nucleic acids, and the nucleic acids are adsorbed by the magnetic beads. In the washing step, the reagent tank 40 pre-stores the washing solution, and the washing solution uses a buffer solution that removes contaminants such as proteins and polysaccharides. In the elution step, the reagent tank 40 pre-stores the elution solution, and the elution solution uses a low-salt solution to separate the nucleic acids from the magnetic beads, obtaining high-purity nucleic acids.

[0030] The steps for the first adsorption of magnetic beads are as follows: Step 1, install the reagent kit 4 at the output end of the linear drive device 3; Step 2, before testing, place the execution sleeve 11 into the reagent tank 40 of the reagent kit 4. The rotating shaft 12 is connected to the execution sleeve 11, and the execution sleeve 11 will not detach from the rotating shaft 12 without external force; Step 3, the first lifting drive mechanism 15 drives the first lifting seat 14 to descend, and the rotating shaft 12 is pressed into the execution sleeve 11. The buckle arm 112 at the top of the execution sleeve 11 clamps the rotating shaft 12; Step 4, the rotation drive mechanism 13 drives the execution sleeve 11 and the rotating shaft 12 to rotate together, and the magnetic beads can float to various positions in the reagent tank 40 for convenient adsorption (Steps 3 and 4 are magnetic bead adsorption operations) of nucleic acids; Step 5, the second lifting drive mechanism 18 drives the second lifting seat 17 to descend, and the magnetic rod extends into the execution sleeve 11 to adsorb the magnetic beads on the outer wall of the execution sleeve 11.

[0031] Releasing the magnetic beads: Just drive the second lifting drive mechanism 18 to drive the second lifting seat 17 and the magnetic rod to rise, and the magnetic beads can be released. This method can be used for the washing and elution steps that require the release of magnetic beads.

[0032] In the washing and elution steps, the solution needs to be stirred. The steps for stirring the solution are as follows: The rotation drive mechanism 13 drives the rotating shaft 12 to rotate, and the rotating shaft 12 drives the execution sleeve 11 to rotate, realizing the cleaning of the reagent tank 40 and the stirring of the magnetic beads.

[0033] The process from the washing step to the elution step is as follows: First, the second lifting drive mechanism 18 drives the second lifting seat 17 to rise, and at the same time, the first lifting drive mechanism 15 drives the first lifting seat 14 to rise. The execution sleeve 11 is separated from the reagent kit 4. The magnetic rod is located in the execution sleeve 11, and the magnetic beads are on the execution sleeve 11; Then, the linear drive device 3 drives the reagent tank 40 for the next row of cleaning in the reagent kit 4 to align with the execution sleeve 11; Then, the second lifting drive mechanism 18 drives the second lifting seat 17 to rise, and the magnetic rod is separated from the execution sleeve 11, and the magnetic beads are located in the reagent tank 40; Then, the rotation drive mechanism 13 drives the rotating shaft 12 to rotate, and the rotating shaft 12 drives the execution sleeve 11 to rotate, realizing the stirring of the nucleic acids and the elution solution in the reagent tank 40. The elution solution separates the nucleic acids from the magnetic beads, so that the nucleic acids on the magnetic beads are transferred to the reagent tank 40 with the elution solution, achieving the purification purpose; Finally, after adsorbing the magnetic beads and transferring the magnetic beads, the purified nucleic acids can be obtained.

[0034] Transfer of used magnetic beads and execution sleeves: The second lifting drive mechanism 18 drives the magnetic rod 16 and the second lifting seat 17 to descend. The magnetic rod 16 extends into the execution sleeve 11, and the magnetic rod 16 adsorbs the magnetic beads outside the execution sleeve 11. Subsequently, both the second lifting drive mechanism 18 and the first lifting drive mechanism 15 operate to drive the magnetic rod 16 and the execution sleeve 11 to extend into the reagent tank for storing the used magnetic beads 16 and the execution sleeve 11. Finally, the second lifting drive mechanism 18 drives the magnetic rod 16 to descend, and the magnetic rod 16 impacts the execution sleeve 11, causing the execution sleeve 11 stuck on the rotating shaft 12 to separate. The operation of separating the execution sleeve 11 facilitates the alignment of the next set of reagent tanks 40 and the execution sleeve 11 with the rotating shaft 12, facilitating the execution of the next purification operation. During the above process, the purification operation of a set of reagent tanks 40 can use a row of execution sleeves 11, avoiding mutual contamination between two purification operations.

[0035] The execution sleeve 11 includes: a sleeve body 111 and a buckle arm 112. The sleeve body 111 extends into the reagent tank 40. At least two buckle arms 112 extend from the top of the sleeve body 111. There is a gap between adjacent buckle arms 112, and the buckle arm 112 can be in snap-fit with the rotating shaft 12. It is realized that the rotating shaft 12 is clamped by the buckle arm 112, so that the rotating shaft 12 can drive the execution sleeve 11 to lift through the buckle arm 112.

[0036] An annular groove 120 is formed on the outer wall of the rotating shaft 12, and a protrusion 113 protrudes from the buckle arm 112. The protrusion 113 is in snap-fit with the inner wall of the annular groove 120. The snap-fit is realized to make the execution sleeve 11 clamped on the rotating shaft 12. Of course, the rotating shaft 12 and the execution sleeve 11 can also be other pluggable and detachable connections, such as being connected by friction and separated after pressing down the execution sleeve 11.

[0037] The rotating shaft 12 includes: a first shaft sleeve and a second shaft sleeve. The first shaft sleeve is rotatably installed on the first lifting seat 14. The first shaft sleeve is used to connect to the rotation drive mechanism 13. The bottom end of the first shaft sleeve is connected with the second shaft sleeve, and the annular groove 120 is formed on the second shaft sleeve. The separate setting makes the rotation of the first shaft sleeve smoother, and the second shaft sleeve is for forming the annular groove 120.

[0038] A ball bearing is installed between the first shaft sleeve and the lifting seat. This enables the first shaft sleeve to rotate smoothly relative to the first lifting seat 14.

[0039] The stirring and adsorbing magnetic bead execution device 1, the mounting frame 2, the linear drive device 3, and the reagent kit 4 are all installed in a box body 5. A window is opened on one side of the box body 5, and the window is opened and closed by a window door 50. The detection is performed in a fully enclosed manner to avoid external interference with the detection.

[0040] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and not to limit them. Although the present invention has been described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that the technical solutions of the present invention can be modified or equivalently replaced without departing from the spirit and scope of the technical solutions of the present invention, and they should all be covered within the scope of the claims of the present invention.

Claims

1. An automatic nucleic acid extraction and purification instrument with an execution set, characterized in that, Including: A stirring and adsorbing magnetic bead execution device (1), a mounting rack (2), a linear driving device (3), and a reagent kit (4); One side of the mounting rack (2) is provided with a stirring and adsorbing magnetic bead execution device (1), a linear driving device (3) is installed below the stirring and adsorbing magnetic bead execution device (1), the output end of the linear driving device (3) is detachably installed with a reagent kit (4), and the linear driving device (3) is used to drive the reagent kit (4) to align with the execution end of the stirring and adsorbing magnetic bead execution device (1); The stirring and adsorbing magnetic bead execution device (1) includes: an execution sleeve (11), a rotating shaft (12), a rotation driving mechanism (13), a first lifting seat (14), a first lifting driving mechanism (15), a magnetic rod (16), a second lifting seat (17), and a second lifting driving mechanism (18); The reagent kit (4) is provided with a reagent groove (40), the reagent groove (40) is for the execution sleeve (11) to extend into, the reagent groove (40) is used to store reagents and magnetic beads, the rotating shaft can be connected to the execution sleeve after being inserted into the execution sleeve, the rotating shaft (12) can drive the execution sleeve (11) to lift after extending into the execution sleeve (11), the rotating shaft (12) is rotatably installed on the first lifting seat (14), a rotation driving mechanism (13) is installed on the first lifting seat (14), the rotation driving mechanism (13) is used to drive the rotating shaft (12) and the execution sleeve (11) to rotate, the first lifting seat (14) can lift relative to the mounting rack (2), a first lifting driving mechanism (15) is installed on the mounting rack (2), and the output end of the first lifting driving mechanism (15) is connected to the first lifting seat (14); A liftable second lifting seat (17) is installed on the mounting rack (2), the second lifting seat (17) is fixed to the output end of the second lifting driving mechanism (18), the second lifting driving mechanism (18) is installed on the first lifting seat (14) or the mounting rack (2), a magnetic rod (16) is installed on the second lifting seat (17), the magnetic rod (16) can extend into the execution sleeve (11), and the second lifting driving mechanism (18) can drive the magnetic rod to impact the execution sleeve to disengage from the rotating shaft.

2. The automatic nucleic acid extraction and purification instrument with an execution sleeve that can be automatically detached according to claim 1, characterized in that, The execution sleeve (11) includes: a sleeve body (111) and a buckle arm (112), the sleeve body (111) extends into the reagent groove (40), at least two buckle arms (1) extend from the top of the sleeve body (111), there is a gap between adjacent buckle arms (112), and the buckle arm (112) can be in buckle fit with the rotating shaft (12).

3. The nucleic acid extraction and purification instrument with an automatically detachable execution sleeve according to claim 2, wherein, An annular groove (120) is formed on the outer wall of the rotating shaft (12), a protrusion (113) protrudes from the buckle arm (112), and the protrusion (113) is in buckle fit with the inner wall of the annular groove (120).

4. The automatic nucleic acid extraction and purification instrument with an execution sleeve that can be automatically detached according to claim 3, characterized in that, The rotating shaft (12) includes: a first shaft sleeve and a second shaft sleeve, the first shaft sleeve is rotatably installed on the first lifting seat (14), the first shaft sleeve is used to connect to the rotation driving mechanism (13), the bottom end of the first shaft sleeve is connected with a second shaft sleeve, and the annular groove (120) is formed on the second shaft sleeve.

5. The nucleic acid extraction and purification instrument with an automatically detachable execution sleeve according to claim 4, characterized in that, A ball bearing is installed between the first shaft sleeve and the lifting seat.

6. The automated nucleic acid extraction and purification instrument with an automatically detachable execution set according to any one of claims 1 to 5, characterized in that The stirring and adsorbing magnetic bead execution device (1), mounting bracket (2), linear drive device (3), and reagent kit (4) are all installed in a box body (5). A window is provided on one side of the box body (5), and the window is opened and closed by a window door (50).

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