Centrifugal force microscopic dynamic simulation and observation device

Abstract

The invention provides a centrifugal force microscopic dynamic simulation and observation device, and the device comprises a centrifugal force module which is used for providing centrifugal force; the multi-dimensional microscope module is fixedly arranged on the centrifugal force module, and the multi-dimensional microscope module comprises at least two microscope sub-modules and is used for carrying out multi-dimensional dynamic observation on the sample to be detected; the control module is in communication connection with the centrifugal force module and the multi-dimensional microscope module and used for controlling operation of the centrifugal force module and the multi-dimensional microscope module, and the problems that a traditional centrifugal force microscopic observation technology can only provide one-dimensional observation in the single axial direction, complex deformation and mechanical behaviors in a two-dimensional space cannot be analyzed, and the observation precision is poor are solved. According to the method, the observation dimension of microscopic behaviors of the sample in a complex force field is greatly expanded, and synchronous capture of two-dimensional or three-dimensional dynamic information of the sample in a centrifugal state is realized; meanwhile, the intelligent integration of the control module significantly improves the experiment efficiency and precision.

Description

Technical Field

[0001] The present invention relates to the technical field of scientific instruments, and particularly relates to a centrifugal force microscopic dynamic simulation and observation device. Background Art

[0002] In the macroscopic field, centrifugal experimental devices based on hypergravity technology are essential experimental means for studying the large-time and large-space evolution of rock and soil masses and deep-earth materials, and accelerating the phase separation of substances. Hypergravity refers to a physical environment where the gravitational acceleration is greater than the conventional gravitational field of the Earth (1g). The centrifugal force generated by a centrifuge can effectively simulate the hypergravity field. This technology demonstrates two core values: one is the "scale effect", for example, in a 1000g hypergravity field, a 1-cm-thick geological fault can equivalently simulate the stress distribution and fracture process of a 100-m-thick fault under natural gravity; the other is the "time compression effect", for example, when conducting an experiment on the migration of soil pollutants on a hypergravity centrifuge, the migration process that takes thousands of years in nature can be compressed to be completed in a few days. Currently, hypergravity centrifugal equipment is usually large scientific installations, with the following deficiencies: high cost, large volume, inability to observe the changes of samples in real time and dynamically, or only providing one-dimensional observation in a single axial direction.

[0003] In the field of microscopic scale research, single-molecule force spectroscopy technology is a key tool for studying the mechanical behavior of biomolecules / cell deformation. By applying a controllable external force and observing the response of molecules / cells, it reveals important biological mechanisms such as the mechanical properties of nucleic acids, protein folding, and cell deformation. Existing mainstream technologies include optical tweezers, magnetic tweezers, and atomic force microscopes, which have limitations such as high cost, low throughput, and complex operation. As an emerging technology, the centrifugal force microscopic dynamic simulation and observation device realizes high-throughput single-molecule experiments through the combination of a centrifugal force field and microscopic imaging. However, the current centrifugal force microscopic dynamic simulation and observation device has the following problems: only providing one-dimensional observation in a single axial direction, unable to analyze complex mechanical behaviors in two-dimensional space, and the device assembly process is cumbersome, components such as optical elements are not integrated into a closed space, resulting in low stability, difficult sample loading, and the provision of centrifugal force relying on commercial centrifuges, etc.

[0004] Therefore, the existing technology still needs to be further developed. Summary of the Invention

[0005] The purpose of the present invention is to overcome the above technical deficiencies and provide a centrifugal force microscopic dynamic simulation and observation device to solve the problems existing in the prior art.

[0006] To achieve the above technical purpose, the present invention provides a centrifugal force microscopic dynamic simulation and observation device, including: A centrifugal force module for providing centrifugal force; A multi-dimensional microscope module is fixedly arranged on the centrifugal force module. The multi-dimensional microscope module includes at least two microscope sub-modules for performing multi-dimensional dynamic observation on a sample to be measured. A control module is communicatively connected to both the centrifugal force module and the multi-dimensional microscope module, and is used to control the operation of the centrifugal force module and the multi-dimensional microscope module.

[0007] Specifically, the centrifugal force module includes a fixed base and a centrifugal component. The fixed base includes a fixed bottom plate and a fixed frame. The centrifugal component includes a centrifugal motor and a rotating rod. The rotating rod is connected to the rotating shaft of the centrifugal motor, and the centrifugal motor is fixed on the fixed bottom plate through the fixed frame. The multi-dimensional microscope module is fixedly arranged on the rotating rod.

[0008] Specifically, each microscope sub-module includes a housing, an optical component and a sleeve component. The optical component and the sleeve component are arranged inside the housing, and the optical component is fixedly connected to the sleeve component.

[0009] Specifically, the optical component includes a glass slide and a sample holder. The sleeve component includes a sample holder sleeve. During use, the glass slide is inserted into the sample holder, and the sample holder is fixedly arranged in the sample holder sleeve.

[0010] Specifically, the sample holder sleeve includes: A cylindrical main body having an accommodation cavity inside for placing a glass slide. At least two optical interfaces penetrating through the side wall of the cylindrical main body. The at least two optical interfaces are all communicated with the accommodation cavity and are distributed along the circumferential direction of the cylindrical main body. The at least two optical interfaces are used to connect each microscope sub-module in the multi-dimensional microscope module.

[0011] Specifically, when the multi-dimensional microscope module is a two-dimensional microscope module, the at least two microscope sub-modules include a first microscope sub-module and a second microscope sub-module. The first microscope sub-module and the second microscope sub-module are arranged in mutually orthogonal directions to simultaneously perform two-dimensional orthogonal direction dynamic observation on the sample to be measured.

[0012] Specifically, the first microscope sub-module includes a first sleeve component, and the second microscope sub-module includes a second sleeve component. The first sleeve component and the second sleeve component are vertically coupled and connected through a sample holder sleeve.

[0013] Specifically, when the multi-dimensional microscope module is a three-dimensional microscope module, the at least two microscope sub-modules include a first microscope sub-module, a second microscope sub-module, and a third microscope sub-module. The first microscope sub-module, the second microscope sub-module, and the third microscope sub-module are orthogonal to each other in spatial orientation, forming a three-dimensional rectangular coordinate system for dynamically observing the sample to be measured in three orthogonal directions.

[0014] Specifically, the first microscope sub-module includes a first sleeve assembly, the second microscope sub-module includes a second sleeve assembly, and the third microscope sub-module includes a third sleeve assembly; The first sleeve assembly, the second sleeve assembly, and the third sleeve assembly are vertically coupled and connected through a sample carrier sleeve.

[0015] Specifically, the control module includes control software, a centrifugal motor control board, and a WiFi module; The centrifugal motor control board is used to control the rotation speed and direction of the centrifugal motor, and the WiFi module is used to transmit the photos of the sample to be measured taken by the multi-dimensional microscope module to the control software.

[0016] Beneficial effects: The present invention provides a centrifugal force microscopic dynamic simulation and observation device, including a centrifugal force module for providing centrifugal force and a multi-dimensional microscope module including at least two microscope sub-modules for dynamically observing the sample to be measured in multiple dimensions; the operation of the centrifugal force module and the multi-dimensional microscope module is controlled by a control module, solving the technical problems that traditional microscopic observation techniques can only provide one-dimensional observation in a single axial direction in a centrifugal force field environment, cannot analyze complex deformations and mechanical behaviors in a two-dimensional space, and components such as optical elements are not integrated into a closed space, resulting in low stability. It greatly improves the observation dimension of the microscopic behavior of the sample in the centrifugal force field and the data acquisition ability. Through the collaborative design of the spatial orthogonal layout of the multi-dimensional microscope module and the optical interface of the sample carrier sleeve, synchronous capture of three-dimensional dynamic information of the sample under centrifugation is achieved; at the same time, the intelligent integration of the control module significantly improves the experimental efficiency and accuracy, and greatly improves the intelligence, usability, and reliability of the present invention. Description of the drawings

[0017] Figure 1 is the overall structure diagram of the two-dimensional centrifugal force microscopic dynamic simulation and observation device provided in the specific embodiment of the present invention; Figure 2 is the structural schematic diagram of the centrifugal force module provided in the specific embodiment of the present invention; Figure 3 is the structural schematic diagram of the one-dimensional microscope module provided in the specific embodiment of the present invention; Figure 4It is an exploded view of the one-dimensional microscope module provided in the specific embodiment of the present invention; Figure 5 It is a schematic structural diagram of the sample carrier and the glass slide provided in the specific embodiment of the present invention; Figure 6 It is a schematic structural diagram of the sample carrier sleeve provided in the specific embodiment of the present invention; Figure 7 It is a schematic structural diagram of the two-dimensional microscope module provided in the specific embodiment of the present invention; Figure 8 It is an exploded view of the two-dimensional microscope module provided in the specific embodiment of the present invention; Figure 9 It is a schematic composition diagram of the control module provided in the specific embodiment of the present invention; Figure 10 It is an overall structure diagram of the three-dimensional centrifugal force microscopic dynamic simulation and observation device provided in the specific embodiment of the present invention; Figure 11 It is a schematic structural diagram of the three-dimensional microscope module provided in the specific embodiment of the present invention; Figure 12 It is an exploded view of the three-dimensional microscope module provided in the specific embodiment of the present invention; Among them, the reference numerals of the above-mentioned drawings are as follows: 1. Centrifugal force module; 2. Two-dimensional microscope module; 3. Three-dimensional microscope module; 11. Fixed base; 12. Centrifugal component; 13. Counterweight; 111. Fixed bottom plate; 112. Fixed frame; 121. Centrifugal motor; 122. Rotating rod; 123. Rotating shaft; 5. Housing; 6. Optical component; 7. Sleeve component; 8. Slide; 9. Sample carrier; 10. Sample carrier sleeve; 14. Cylindrical body; 15. Accommodation cavity; 16. Optical interface; 21. First housing; 22. Second housing; 23. Third housing; 61. Light source lamp holder; 62. Light source lamp; 63. Optical diffuser; 64. Fresnel lens; 65. Objective lens; 66. Camera; 71. Light source lamp sleeve; 72. Optical diffuser sleeve; 73. Fresnel lens sleeve; 74. Objective lens sleeve; 75. Camera connection sleeve; 611. First light source lamp holder; 621. First light source lamp; 631. First optical diffuser; 641. First Fresnel lens; 651. First objective lens; 661. First camera; 711. First light source lamp sleeve; 721. First optical diffuser sleeve; 731. First Fresnel lens sleeve; 741. First objective lens sleeve; 751. First camera connection sleeve; 612. Second light source lamp holder; 622. Second light source lamp; 632. Second optical diffuser; 642. Second Fresnel lens; 652. Second objective lens; 662. Second camera; 712. Second light source lamp sleeve; 722. Second optical diffuser sleeve; 732. Second Fresnel lens sleeve; 742. Second objective lens sleeve; 752. Second camera connection sleeve; 613. Third light source lamp holder; 623. Third light source lamp; 633. Third optical diffuser; 643. Third Fresnel lens; 653. Third objective lens; 663. Third camera; 713. Third light source lamp sleeve; 723. Third optical diffuser sleeve; 733. Third Fresnel lens sleeve; 743. Third objective lens sleeve; 753. Third camera connection sleeve. Detailed implementation

[0018] In order to enable those skilled in the art to better understand the technical solutions of the present invention, the technical solutions of the present invention will be clearly and completely described below in conjunction with the drawings of the present invention. Based on the embodiments in this application, other similar embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the scope of protection of this application. In addition, the directional terms mentioned in the following embodiments, such as "up", "down", "left", "right", etc., are only references to the directions in the drawings. Therefore, the directional terms used are for illustration rather than to limit the present invention.

[0019] The present invention will be further described below in conjunction with the drawings and preferred embodiments.

[0020] Embodiment 1 Please refer to Figure 1, this embodiment provides a centrifugal force microscopic dynamic simulation and observation device, including a centrifugal force module 1, a multi-dimensional microscope module, and a control module. Among them, the centrifugal force module 1 is used to provide centrifugal force; the multi-dimensional microscope module is fixedly arranged on the centrifugal force module 1, and the multi-dimensional microscope module includes at least two microscopic mirror sub-modules for multi-dimensional dynamic observation of the sample to be measured; the control module is communicatively connected to both the centrifugal force module 1 and the multi-dimensional microscope module for controlling the operation and signal transmission of the centrifugal force module 1 and the multi-dimensional microscope module.

[0021] It can be understood that according to the above technical solution, the centrifugal force microscopic dynamic simulation and observation device provided in this embodiment solves the technical problems of traditional microscopic observation technology that can only provide one-dimensional observation in a single axial direction in a centrifugal force field environment, cannot analyze complex deformations and mechanical behaviors in a two-dimensional space, and components such as optical elements are not integrated into a closed space, resulting in low stability. It realizes multi-dimensional dynamic observation of the sample to be measured under centrifugal conditions.

[0022] See Figure 2 , in this embodiment, the centrifugal force module 1 includes a fixed base 11 and a centrifugal component 12. The fixed base 11 includes a fixed bottom plate 111 and a fixed frame 112. The centrifugal component 12 includes a centrifugal motor 121 and a rotating rod 122. The rotating rod 122 is connected to the rotating shaft 123 of the centrifugal motor 121. The centrifugal motor 121 is placed on the fixed bottom plate 111 and fixed to the fixed bottom plate 111 through the fixed frame 112 to ensure that the centrifugal force microscopic dynamic simulation and observation device does not shake or wobble during operation, ensuring that the entire device remains stable during high-speed rotation. The multi-dimensional microscope module is fixedly arranged on the rotating rod 122, so that when the rotating rod 122 rotates at high speed, it drives the multi-dimensional microscope to rotate together. According to the above technical solution, through the design of a highly stable fixed structure and fixing the multi-dimensional microscope to the centrifugal force module, in-situ, stable, multi-dimensional, and synchronized dynamic microscopic observation of the sample is achieved in a precisely controllable centrifugal force field.

[0023] Furthermore, the centrifugal motor 121 uses a high-speed or ultra-high-speed (>2000 r / min) motor to provide sufficient centrifugal force to deform the sample to be measured placed in the multi-dimensional microscope. Different magnitudes of centrifugal force can be provided in the following two ways: on the one hand, different centrifugal forces are generated by setting different rotation speeds; on the other hand, the multi-dimensional microscope module can be fixed at different positions on the rotating rod 122, thereby being able to generate different centrifugal forces.

[0024] Furthermore, the rotating rod 122 can be fixed to the rotating shaft 123 of the centrifugal motor 121 by one of the following two fixing methods or a combination of the two methods: Method 1: The rotating rod 122 is screwed onto the end of the rotating shaft 123 of the centrifugal motor 121 through threads and is fixed at both the upper and lower ends by two nuts. Method 2: The cross-shaped nut is screwed onto the end of the rotating shaft 123 of the centrifugal motor 121. At the same time, the cross is embedded into the cross-shaped groove of the rotating rod 122 and is fixedly connected to the rotating rod 122 by bolts.

[0025] Preferably, the fixed base plate 111 can adopt a platform with a relatively large weight, such as an optical platform with shock absorption effect or other workpieces. The fixed frame 112 is made of aluminum profiles and aluminum profile corner pieces. The aluminum profile frame can provide strong support to resist deformation force. At the same time, the modularity of the aluminum profile and the precise interface characteristics of the optical platform significantly improve the usability, adjustability, expandability and installation accuracy of the device, laying a solid physical foundation for high-resolution and multi-dimensional microscopic dynamic observation under high-speed centrifugation.

[0026] See Figure 2 , in some specific embodiments, the centrifugal module further includes a counterweight 13. The counterweight 13 is fixedly arranged on the other side of the rotating rod 122 and is symmetrically arranged with respect to the end of the multi-dimensional microscope module relative to the centrifugal motor 121. The counterweight 13 is used to maintain the balance of the entire centrifugal force microscopic dynamic simulation and observation device to ensure the stability of the multi-dimensional microscope module during high-speed rotation. In addition, a same multi-dimensional microscope module can also be placed on the other side of the rotating rod 122, that is, at the symmetrical position of the multi-dimensional microscope module, to ensure the balance and stability of the multi-dimensional microscope module during the high-speed rotation of the rotating rod 122.

[0027] See Figure 3 , in this embodiment, the microscopic mirror module includes a housing 5, an optical component 6 and a sleeve component 7. The optical component 6 and the sleeve component 7 are arranged inside the housing 5, and the optical component 6 is fixedly connected to the sleeve component 7.

[0028] Specifically, see Figure 3 , the housing 5 includes a first housing 21 and a second housing 22, which jointly enclose the optical component 6 and the sleeve component 7.

[0029] Specifically, see Figure 4 , the optical component 6 includes a glass slide 8 and a sample holder 9. The sleeve component 7 includes a sample holder sleeve 10. During use, the glass slide 8 is embedded into the sample holder 9, and the sample holder 9 is fixedly arranged in the sample holder sleeve 10. It realizes in-situ and synchronous multi-dimensional dynamic observation of the sample to be measured in the centrifugal force field, ensures that the observation process does not interfere with the natural state of the sample, and greatly improves the acquisition efficiency and reliability of multi-dimensional data.

[0030] Furthermore, see Figure 4 and Figure 5, the optical component 6 further includes a light source lamp holder 61, a light source lamp 62, an optical diffuser 63, a Fresnel lens 64, an objective lens 65, and a camera 66. The sleeve assembly 7 further includes a light source lamp sleeve 71, an optical diffuser sleeve 72, a Fresnel lens sleeve 73, an objective lens sleeve 74, and a camera connection sleeve 75. Among them, the light source lamp 62 is used to provide illumination light, the light source lamp holder 61 is used to mount and fix the light source lamp 62, the light source lamp 62 is installed (embedded) in the light source lamp holder 61, the optical diffuser 63 is located behind the light source, and is used to homogenize the light emitted by the light source, reducing glare and hot spots. The optical diffuser 63 is fixedly arranged in the optical diffuser sleeve 72. The Fresnel lens 64 is fixed in the Fresnel lens sleeve 73. The Fresnel lens 64 is usually used as a condenser lens to converge the diffused light onto the sample area. Two glass slides 8 are embedded in the sample holder 9, as Figure 5 shown. The two glass slides are used to hold the sample to be observed. The sample holder 9 is a specially designed fixture or bracket for fixing the two glass slides 8 with the sample. The sample holder 9 is fixedly inserted into the sample holder sleeve 10, facilitating the quick installation and replacement of the sample. The objective lens 65 is fixed in the objective lens sleeve 74. The objective lens 65 is placed close to the sample, responsible for collecting the light emitted by the sample or transmitted through the sample and performing primary magnified imaging. The camera 66 is located at the end of the imaging optical path (behind the objective lens), and is used to receive the optical image formed by the objective lens and convert it into an electrical signal (image). The light source lamp holder 61 and all the sleeves are threadedly connected in sequence from left to right, forming a one-dimensional microscope module.

[0031] See Figure 6 , in this embodiment, the sample holder sleeve 10 includes a cylindrical main body 14 and at least two optical interfaces 16. Among them, the cylindrical main body 14 has a receiving cavity 15 for placing the glass slide 8; and at least two optical interfaces 16 penetrate through the side wall of the cylindrical main body 14, are all communicated with the receiving cavity 15, and are distributed along the circumferential direction of the cylindrical main body 14; among them, each optical interface 16 includes a light-transmitting area for allowing the optical path of the corresponding connected microscope sub-module to pass through and reach the sample to be measured in the receiving cavity 15; Specifically, at least two optical interfaces 16 are used to connect each microscope sub-module in the multi-dimensional microscope module. The positions of the optical interfaces 16 match the observation optical paths of each microscope sub-module in the multi-dimensional microscope module to achieve dynamic observation of different orientations of the sample in the rotating state. According to the above technical solution, the sample holder sleeve in this embodiment realizes multi-angle optical path penetration through the circumferentially distributed optical interface design, enabling multiple microscope sub-modules to synchronously, in-situ, and multi-dimensionally dynamically observe the deformation of the sample in the centrifugal force field while ensuring the high-speed rotation stability.

[0032] See Figure 7, in this embodiment, the multi-dimensional microscope module is a two-dimensional microscope module 2. The two-dimensional microscope module 2 includes a first microscope sub-module and a second microscope sub-module. The first microscope sub-module and the second microscope sub-module are the same microscope sub-module. The first microscope sub-module and the second microscope sub-module are arranged in mutually orthogonal directions to simultaneously perform dynamic observation of the sample to be measured in two-dimensional orthogonal directions. By orthogonally arranging two identical microscope sub-modules, the two-dimensional microscope module 2 of this embodiment synchronously obtains real-time deformation data of the sample in mutually perpendicular directions in the centrifugal force field, completely eliminating the observation perspective deviation caused by rotation, providing high precision for research on material stress distribution, biological cell deformation, etc., and greatly improving the observation efficiency and data reliability.

[0033] Specifically, referring to Figure 7 and Figure 8 , the first microscope sub-module includes a first sleeve assembly, and the second microscope sub-module includes a second sleeve assembly. The first sleeve assembly and the second sleeve assembly are vertically coupled and connected through the sample carrier sleeve 10. According to the above technical solution, mechanical coupling is used to ensure strict orthogonality of the double optical paths, eliminate the errors of independent alignment and adjustment, and maintain zero deviation in observation during high-speed rotation; the first microscope sub-module and the second microscope sub-module share the same sample loading cavity, and the control module can trigger the two cameras simultaneously to achieve millisecond-level synchronous exposure, capture the instantaneous correlation state of orthogonal deformation, create a confocal synchronous imaging environment, and make the two-dimensional dynamic data have spatio-temporal consistency.

[0034] It should be further noted that to expand the observation dimension, on the basis of the one-dimensional microscope module, a set of identical microscope modules are arranged along the orthogonal direction, thus forming the two-dimensional microscope module 2. As Figure 7 shown, in the two-dimensional microscope module 2, the housing 5 includes a first housing 21 and a second housing 22, which jointly enclose the optical component 6 and the sleeve assembly 7. The optical component 6 includes a first optical component and a second optical component. As Figure 8As shown in the figure, the first optical component includes a first light source lamp holder 611, a first light source lamp 621, a first optical diffuser 631, a first Fresnel lens 641, a slide 8, a sample holder 9, a first objective lens 651, and a first camera 661. The second optical component includes a second light source lamp holder 612, a second light source lamp 622, a second optical diffuser 632, a second Fresnel lens 642, a slide 8, a sample holder 9, a second objective lens 652, and a second camera 662. The sample holder 9 forms a 45° angle with both the x-direction microscope sub-module and the y-direction microscope sub-module. The sleeve assembly 7 includes a first sleeve assembly and a second sleeve assembly. The first sleeve assembly includes a first light source lamp sleeve 711, a first optical diffuser sleeve 721, a first Fresnel lens sleeve 731, a sample holder sleeve 10, a first objective lens sleeve 741, and a first camera connection sleeve 751. The second sleeve assembly includes a second light source lamp sleeve 712, a second optical diffuser sleeve 722, a second Fresnel lens sleeve 732, a sample holder sleeve 10, a second objective lens sleeve 742, and a second camera connection sleeve 752. The first sleeve assembly and the second sleeve assembly are vertically coupled and connected through the sample holder sleeve 10 therein. The sample holder sleeve 10 includes two optical interfaces 16, which are respectively connected to the first microscope sub-module and the second microscope sub-module, so as to realize two-dimensional dynamic observation of the sample to be measured in a rotating state.

[0035] It should be noted here that the first microscope sub-module is the same as the second microscope sub-module. The first light source lamp 621 is embedded in the first light source lamp holder 611. The first optical diffuser 631 is fixed in the first optical diffuser sleeve 721. The first Fresnel lens 641 is fixed in the first Fresnel lens sleeve 731. The slide 8 is embedded in the sample holder 9. The sample holder 9 is inserted and fixed in the sample holder sleeve 10. The first objective lens 651 is screwed tightly in the first objective lens sleeve 741. The first light source lamp holder 611 and the sleeves of all the first microscope sub-modules are sequentially connected by threads in the x-direction and the y-direction. The connection methods of the components of the second microscope sub-module are the same as those of the components of the first microscope sub-module.

[0036] It can be understood that in this embodiment, for the two-dimensional microscope module, the sample carrier sleeve 10 includes a cylindrical main body 14 and two optical interfaces 16. Among them, the cylindrical main body 14 has a receiving cavity 15 for placing the slide 8; the two optical interfaces 16 penetrate through the side wall of the cylindrical main body 14, communicate with the receiving cavity 15, and are distributed along the circumference of the cylindrical main body 14; each optical interface 16 includes a light-transmitting area for allowing the optical path of the corresponding connected microscope sub-module to pass through and reach the sample to be measured in the receiving cavity 15; the two optical interfaces 16 are used to connect the first microscope sub-module and the second microscope sub-module in the two-dimensional microscope module. The positions of the two optical interfaces 16 match the observation optical paths of the first microscope sub-module and the second microscope sub-module in the two-dimensional microscope module to achieve dynamic two-dimensional observation of the sample in a rotating state.

[0037] See Figure 9 , in this embodiment, the control module includes control software, a centrifugal motor control board, and a WiFi module. The centrifugal motor control board is communicatively connected to the centrifugal motor 121 and is used to control the rotation speed and direction of the centrifugal motor 121. The WiFi module communicatively connects the camera and the control software and is used to wirelessly transmit the photos of the sample to be measured taken by the two-dimensional microscope module 2 to the control software; according to the above technical solution, the control module remotely and precisely regulates the rotation speed / direction and synchronously transmits back multi-dimensional microscopic images through wireless communication with the integrated control architecture, completely eliminating the safety risks and data delays of manual intervention in traditional observations; relying on the software platform to complete the spatio-temporal correlation of centrifugal parameters-dynamic deformation data, providing a closed-loop analysis ability for the material response under complex force fields. In addition, the camera can be a camera with a built-in WiFi chip without an additional WiFi module.

[0038] See Figure 9 , further, the controller module in this embodiment is designed to control the operation of the two-dimensional centrifugal force microscopic dynamic simulation and observation device in this embodiment and transmit image signals. In addition to the control software, the centrifugal motor control board, and the WiFi module, the control module also includes a power supply and a boost module. Among them, the power supply can be a lithium battery, and the boost module adjusts the output voltage of the power supply to the rated voltage values of the WiFi module, the light source lamp holder 61, and the camera 66. The light source lamp holder 61 can be an LED lamp holder, so as to realize the power supply of the two-dimensional microscope module 2.

[0039] The following uses specific examples to illustrate the specific implementation process of the centrifugal force microscopic dynamic simulation and observation device in this embodiment: (1) Sample loading preparation: The operator prepares two slides 8, such as Figure 5As shown in the figure, first, a glass slide 8 is embedded into the single-sided convex platform of the sample holder 9 and pasted on the side of the sample holder 9 with tape. For solid samples, the sample is cut into small thin pieces and placed on the glass slide 8. For liquid samples, a pipette such as a pipettor is used to drop the sample on the glass slide 8, and another glass slide 8 is embedded into the other-sided convex platform of the sample holder 9 and pasted on the side of the sample holder 9, that is, the sample is located in the gap between the two glass slides 8. For samples of single molecules (such as DNA / protein) and micro-nano structures (such as DNA nano-machines), streptavidin and magnetic bead microspheres are required. Streptavidin binds the sample to the glass slide 8, and the magnetic bead microspheres break the sample under centrifugal force to facilitate mechanical property analysis; (2) Positioning the sample holder 9: Insert the sample holder 9 into the accommodation cavity 15 of the sample holder sleeve 10; (3) Replacement of the counterweight 13: Select the corresponding counterweight 13 according to the multi-dimensional microscope module used (two-dimensional microscope module 2 or three-dimensional microscope module 3) to ensure the balance of both ends of the rotating rod 122; (3) Commissioning of the instrument: Turn on the power of the centrifugal motor 121 and the two-dimensional microscope module 2 (or three-dimensional microscope module 3) to ensure that the images can be normally transmitted to the computer (control software); (4) Operation of the instrument: Adjust the speed of the centrifugal motor 121 through the centrifugal motor control board and run the motor. The rotation of the motor drives the rotation of the two-dimensional microscope module 2 (or three-dimensional microscope module 3). The camera 66 takes pictures of the sample in real time and transmits them back to the computer through the WiFi module; (5) Image acquisition: Acquire images through the computer control software. The images cover the process of the sample from the initial state to the disappearance state; (6) Data analysis: Organize the collected images, analyze the time when the sample deforms or dissociates due to centrifugal force and the magnitude of the centrifugal force. By adjusting the speed of the centrifugal motor 121 or the fixed position of the two-dimensional microscope module 2 (or three-dimensional microscope module 3), repeat the operation steps to observe and analyze the influence of different centrifugal forces on the sample; (7) After the experiment, the operator removes the sample and turns off the power of the instrument.

[0040] It should be noted here that this embodiment provides a two-dimensional centrifugal force microscopic dynamic simulation and observation device, including a centrifugal force module for providing centrifugal force and a two-dimensional microscope module for multi-dimensional dynamic observation of a sample to be measured. By using a control module to control the operation of the centrifugal force module 1 and the two-dimensional microscope module, it solves the technical problems of traditional microscopic observation techniques that can only provide one-dimensional observation in a single axial direction in a centrifugal force field environment, cannot analyze complex deformations and mechanical behaviors in a two-dimensional space, and components such as optical elements are not integrated into a closed space, resulting in low stability. It greatly improves the observation dimension of the microscopic behavior of samples in a complex force field and the data acquisition ability. Through the collaborative design of the orthogonal layout of the two-dimensional microscope module and the optical interface of the sample carrier sleeve, synchronous capture of two-dimensional dynamic information of the sample under centrifugation is achieved. At the same time, the intelligent integration of the control module significantly improves the experimental efficiency and accuracy, and greatly improves the intelligence, usability, and reliability of the present invention.

[0041] Embodiment 2 Please refer to Figure 10 , this embodiment provides a three-dimensional centrifugal force microscopic dynamic simulation and observation device. Different from the above-mentioned Embodiment 1, the multi-dimensional microscope module in this embodiment is a three-dimensional microscope module 3. The three-dimensional centrifugal force microscopic dynamic simulation and observation device includes a centrifugal force module 1, a three-dimensional microscope module 3, and a control module. Among them, the centrifugal force module 1 is used to provide centrifugal force, the three-dimensional microscope module 3 is fixedly arranged on the centrifugal force module 1, the three-dimensional microscope module 3 includes a first microscope sub-module, a second microscope sub-module, and a third microscope sub-module, and the three-dimensional microscope module 3 is used for three-dimensional dynamic observation of a sample to be measured. The control module is communicatively connected to both the centrifugal force module 1 and the three-dimensional microscope module 3 and is used to control the operation of the centrifugal force module 1 and the multi-dimensional microscope module. According to the above technical solution, synchronous capture of the three-dimensional space deformation field of the sample is achieved in a high-speed rotating coordinate system, breaking through the planar limitation of traditional centrifugal observation, and revealing the full-dimensional dynamic laws of volume deformation of materials and the evolution of the three-dimensional structure of biological cells under the action of centrifugal force.

[0042] See Figure 6 , in the three-dimensional centrifugal force microscopic dynamic simulation and observation device of this embodiment, the sample carrier sleeve 10 includes a cylindrical main body 14 with an accommodation cavity 15 inside, and the accommodation cavity 15 is used to place a glass slide 8. The sample carrier sleeve 10 further includes three optical interfaces 16, which penetrate the side wall of the cylindrical main body 14 and are all communicated with the accommodation cavity 15, and these three optical interfaces 16 are circumferentially distributed along the cylindrical main body 14. Among them, each optical interface 16 includes a light-transmitting area for allowing the optical path of the corresponding connected microscope sub-module to pass through and reach the sample to be measured in the accommodation cavity 15; Specifically, the three optical interfaces 16 are respectively used to connect the first microscope mirror module, the second microscope mirror module, and the third microscope mirror module in the three-dimensional microscope module 3. The positions of the optical interfaces 16 match the observation optical paths of the microscope mirror modules in the three-dimensional microscope module 3, so as to realize the three-dimensional dynamic observation of the sample to be measured in a rotating state.

[0043] Furthermore, in the three-dimensional centrifugal force microscopic dynamic simulation and observation device in this embodiment, the first microscope mirror module, the second microscope mirror module, and the third microscope mirror module are orthogonal to each other in spatial orientation, forming a three-dimensional rectangular coordinate system to perform dynamic observation of the sample to be measured in three orthogonal directions.

[0044] Specifically, referring to Figure 11 and Figure 12 , the first microscope mirror module includes a first sleeve assembly, the second microscope mirror module includes a second sleeve assembly, the third microscope mirror module includes a third sleeve assembly, and the first sleeve assembly, the second sleeve assembly, and the third sleeve assembly are vertically coupled and connected through the sample carrier sleeve 10. According to the above technical solution, the three-dimensional orthogonal coupling design of the three-dimensional microscope module realizes the three-dimensional dynamic observation of the sample to be measured under the centrifugal force state by the three-way mechanical interlock of the sample carrier sleeve 10, forcibly constraining the spatial orthogonality of the three optical paths with a rigid structure, and synchronously constructing a capture system for the three-dimensional dynamic deformation field in the rotating coordinate system.

[0045] It should be further noted that, in order to expand the observation dimension, on the basis of the first microscope mirror module, two sets of identical microscope modules are arranged along two orthogonal directions of x and y, namely the second microscope mirror module and the third microscope mirror module, thus forming the three-dimensional microscope module 3.

[0046] Referring to Figure 11 , in the three-dimensional centrifugal force microscopic dynamic simulation and observation device of this embodiment, the three-dimensional microscope module 3 includes a housing 5, an optical element assembly, and a sleeve assembly 7 to meet the requirements of three-dimensional observation. Among them, the housing 5 includes a first housing 21, a second housing 22, and a third housing 23, which jointly enclose the optical component 6 and the sleeve assembly 7.

[0047] Referring to Figure 12, in the three-dimensional centrifugal force microscopic dynamic simulation and observation device of this embodiment, the optical element assembly includes a first optical assembly, a second optical assembly, and a third optical assembly. The first optical assembly includes a first light source lamp holder 611, a first light source lamp 621, a first optical diffuser 631, a first Fresnel lens 641, a slide 8, a sample holder 9, a first objective lens 651, and a first camera 661; the second optical assembly includes a second light source lamp holder 612, a second light source lamp 622, a second optical diffuser 632, a second Fresnel lens 642, a slide 8, a sample holder 9, a second objective lens 652, and a second camera 662. The third optical assembly includes a third light source lamp holder 613, a third light source lamp 623, a third optical diffuser 633, a third Fresnel lens 643, a slide 8, a sample holder 9, a third objective lens 653, and a third camera 663. The sample holder 9 forms a 45° angle with both the microscope module in the x direction and the microscope module in the y direction.

[0048] See Figure 12 , in the three-dimensional centrifugal force microscopic dynamic simulation and observation device of this embodiment, the sleeve assembly 7 includes a first sleeve assembly, a second sleeve assembly, and a third sleeve assembly. The first sleeve assembly includes a first light source lamp sleeve 711, a first optical diffuser sleeve 721, a first Fresnel lens sleeve 731, a sample holder sleeve 10, a first objective lens sleeve 741, and a first camera connection sleeve 751. The second sleeve assembly includes a second light source lamp sleeve 712, a second optical diffuser sleeve 722, a second Fresnel lens sleeve 732, a sample holder sleeve 10, a second objective lens sleeve 742, and a second camera connection sleeve 752. The third sleeve assembly includes a third light source lamp sleeve 713, a third optical diffuser sleeve 723, a third Fresnel lens sleeve 733, a sample holder sleeve 10, a third objective lens sleeve 743, and a third camera connection sleeve 753. The first sleeve assembly, the second sleeve assembly, and the third sleeve assembly are vertically coupled and connected through the sample holder sleeve 10 therein. Among them, the first light source lamp 621 is embedded in the first light source lamp holder 611, the first optical diffuser 631 is fixed in the first optical diffuser sleeve 721, the first Fresnel lens 641 is fixed in the first Fresnel lens sleeve 731, the slide 8 is embedded in the sample holder 9, the sample holder 9 is inserted and fixed in the sample holder sleeve 10, the first objective lens 651 is screwed tightly in the first objective lens sleeve 741, and the first light source lamp holder 611 and all the sleeves of the three-dimensional microscope module 3 are sequentially threadedly connected in the x direction, y direction, and z direction. The connection methods of the components in the second microscope sub-module and the third microscope sub-module are the same as those of the components in the first microscope sub-module, realizing three-dimensional dynamic observation of the sample to be measured on the slide 8 in the sample holder sleeve 10.

[0049] See Figure 9, in this embodiment, the control module includes control software, a centrifugal motor control board, and a WiFi module. The centrifugal motor control board is communicatively connected to the centrifugal motor 121 and is used to control the rotation speed and direction of the centrifugal motor 121. The WiFi module is communicatively connected to the control software and is used to wirelessly transmit the photos of the sample to be measured taken by the three-dimensional microscope module 3 to the control software; See Figure 9 , further, the controller module in this embodiment is designed to control the operation of the three-dimensional centrifugal force microscopic dynamic simulation and observation device in this embodiment and transmit image signals. In addition to the control software, the centrifugal motor control board, and the WiFi module, the control module further includes a power supply and a boost module. Among them, the boost module adjusts the output voltage of the power supply to the rated voltage values of the WiFi module, the light source lamp holder 61, and the camera 66, thereby realizing the power supply of the three-dimensional microscope module 3.

[0050] It should be noted here that this embodiment provides a three-dimensional centrifugal force microscopic dynamic simulation and observation device, including a centrifugal force module for providing centrifugal force and a three-dimensional microscope module for multi-dimensional dynamic observation of the sample to be measured; using the control module to control the operation of the centrifugal force module and the three-dimensional microscope module, it solves the technical problems that the traditional microscopic observation technology can only provide one-dimensional observation in a single axial direction in the centrifugal force field environment, cannot analyze complex mechanical behaviors in two-dimensional space, and components such as optical elements are not integrated into a closed space, resulting in low stability. It greatly improves the observation dimension of the microscopic behavior of the sample in the complex force field and the data acquisition ability. Through the collaborative design of the spatial orthogonal layout of the three-dimensional microscope module and the optical interface of the sample holder sleeve, the synchronous capture of the three-dimensional dynamic information of the sample under the centrifugal state is realized; at the same time, the intelligent integration of the control module significantly improves the experimental efficiency and accuracy, and greatly improves the intelligent level, usability, and reliability of the present invention.

[0051] It should be noted that the terms "first", "second", etc. in the specification, claims, and above-mentioned drawings of this application are used to distinguish similar objects and do not necessarily need to describe a specific order or sequence. It should be understood that such used data can be interchanged under appropriate circumstances so that the embodiments of the present application described here can be implemented in an order other than those illustrated or described here. In addition, the terms "including" and "having" and any variations thereof are intended to cover non-exclusive inclusion. For example, a process, method, system, product, or device that includes a series of steps or units does not necessarily have to be limited to those steps or units clearly listed, but may include other steps or units not clearly listed or inherent to these processes, methods, products, or devices.

[0052] The technical features described above can be combined arbitrarily. Although not all possible combinations of these technical features are described, any combination of these technical features should be considered to be covered by this specification, as long as there is no contradiction in such a combination.

[0053] The specific embodiments of the present invention described above do not constitute a limitation on the protection scope of the present invention. Any other corresponding changes and deformations made according to the technical concept of the present invention should be included in the protection scope of the claims of the present invention.

Claims

1. A centrifugal force microscopic dynamic simulation and observation device, characterized in that Comprising: A centrifugal force module (1) for providing centrifugal force; A multi-dimensional microscope module fixedly arranged on the centrifugal force module (1), the multi-dimensional microscope module comprising at least two microscope sub-modules for performing multi-dimensional dynamic observation on a sample to be measured; A control module communicatively connected to both the centrifugal force module (1) and the multi-dimensional microscope module for controlling the operation of the centrifugal force module (1) and the multi-dimensional microscope module.

2. The centrifugal force microscopic dynamic simulation and observation device according to claim 1, characterized in that, The centrifugal force module (1) comprises a fixed base (11) and a centrifugal assembly (12), the fixed base (11) comprises a fixed bottom plate (111) and a fixed frame (112), the centrifugal assembly (12) comprises a centrifugal motor (121) and a rotating rod (122), the rotating rod (122) is connected to the rotating shaft (123) of the centrifugal motor (121), and the centrifugal motor (121) is fixed on the fixed bottom plate (111) through the fixed frame (112); The multi-dimensional microscope module is fixedly arranged on the rotating rod (122).

3. The centrifugal force microscopic dynamic simulation and observation device according to claim 1, wherein The microscope sub-module comprises a housing (5), an optical component (6) and a sleeve component (7), the optical component (6) and the sleeve component (7) are arranged inside the housing (5), and the optical component (6) is fixedly connected to the sleeve component (7).

4. The centrifugal force microscopic dynamic simulation and observation device according to claim 3, characterized in that, The optical component (6) comprises a glass slide (8) and a sample holder (9), the sleeve component (7) comprises a sample holder sleeve (10), and during use, the glass slide (8) is inserted into the sample holder (9), and the sample holder (9) is fixedly arranged in the sample holder sleeve (10).

5. The centrifugal force microscopic dynamic simulation and observation device according to claim 4, characterized in that, The sample holder sleeve (10) comprises: A cylindrical main body (14) having an accommodation cavity (15) inside for placing the glass slide (8); At least two optical interfaces (16) penetrating through the side wall of the cylindrical main body (14), the at least two optical interfaces (16) are all communicated with the accommodation cavity (15), and the at least two optical interfaces (16) are distributed along the circumferential direction of the cylindrical main body (14); The at least two optical interfaces (16) are used for connecting each microscope sub-module in the multi-dimensional microscope module.

6. The centrifugal force microscopic dynamic simulation and observation device according to claim 5, characterized in that, When the multi-dimensional microscope module is a two-dimensional microscope module (2), the at least two microscope sub-modules comprise a first microscope sub-module and a second microscope sub-module, and the first microscope sub-module and the second microscope sub-module are arranged in mutually orthogonal directions to simultaneously perform two-dimensional orthogonal direction dynamic observation on the sample to be measured.

7. The centrifugal force microscopic dynamic simulation and observation device according to claim 6, characterized in that, The first microscope sub-module comprises a first sleeve component, the second microscope sub-module comprises a second sleeve component, and the first sleeve component and the second sleeve component are vertically coupled and connected through the sample holder sleeve (10).

8. The centrifugal force microscopic dynamic simulation and observation device according to claim 5, characterized in that, When the multi-dimensional microscope module is a three-dimensional microscope module (3), the at least two microscope sub-modules include a first microscope sub-module, a second microscope sub-module, and a third microscope sub-module. The first microscope sub-module, the second microscope sub-module, and the third microscope sub-module are orthogonal to each other in spatial orientation, forming a three-dimensional rectangular coordinate system for dynamically observing the sample to be measured in three orthogonal directions.

9. The centrifugal force microscopic dynamic simulation and observation device according to claim 8, characterized in that The first microscope sub-module includes a first sleeve assembly, the second microscope sub-module includes a second sleeve assembly, and the third microscope sub-module includes a third sleeve assembly; The first sleeve assembly, the second sleeve assembly, and the third sleeve assembly are vertically coupled and connected through a sample carrier sleeve (10).

10. The centrifugal force microscopic dynamic simulation and observation device according to claim 2, characterized in that, The control module includes control software, a centrifugal motor control board, and a WiFi module; The centrifugal motor control board is used to control the rotation speed and direction of the centrifugal motor (121), and the WiFi module is used to transmit the photos of the sample to be measured taken by the multi-dimensional microscope module to the control software.

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