A centrifugal force microscopic dynamic simulation and observation device

Abstract

The present invention provides a centrifugal force microscopic dynamic simulation and observation device, comprising: a centrifugal force module for providing centrifugal force; a multidimensional microscope module, fixedly arranged on the centrifugal force module, the multidimensional microscope module including at least two microscope sub-modules, for performing multidimensional dynamic observation of the sample to be measured; a control module, communicatively connected to both the centrifugal force module and the multidimensional microscope module, for controlling the operation of the centrifugal force module and the multidimensional microscope module, solving the technical problems that traditional centrifugal force microscopic observation technology can only provide one-dimensional observation of a single axis, cannot analyze complex deformation and mechanical behavior in two-dimensional space, and components such as optical elements are not integrated into a closed space. The present application greatly expands the observation dimension of the microscopic behavior of samples in complex force fields, and realizes the synchronous capture of two-dimensional or three-dimensional dynamic information of samples under centrifugal state; at the same time, the intelligent integration of the control module significantly improves the experimental efficiency and accuracy.

Description

Technical Field

[0001] The present invention relates to the technical field of scientific instruments, and in particular to a centrifugal force microscopic dynamic simulation and observation device. Background Art

[0002] At the macroscale level, centrifuge experiments based on hypergravity technology are essential experimental tools for studying the spatiotemporal evolution of rock and soil masses and deep Earth materials, as well as for accelerating phase separation. Hypergravity refers to a physical environment in which the gravitational acceleration exceeds Earth's conventional gravity field (1g). The centrifugal force generated by a centifuge can effectively simulate this hypergravity field. This technology demonstrates two core benefits: first, a "scale effect." For example, in a 1000g hypergravity field, a 1cm-thick geological fault can effectively simulate the stress distribution and rupture process of a 100-meter-thick fault under natural gravity. Second, a "time compression effect." For example, experiments on soil contaminant migration conducted in a hypergravity centrifuge can compress a process that would take thousands of years in nature into a matter of days. Currently, hypergravity centrifuges are typically large scientific instruments with several drawbacks: high cost, bulk, inability to dynamically observe sample changes in real time, or limited one-dimensional observation along a single axis.

[0003] In the field of microscopic research, single-molecule force spectroscopy is a key tool for studying the mechanical behavior of biomolecules / cell deformation. By applying controllable external forces and observing the molecular / cellular responses, it reveals important biological mechanisms such as the mechanical properties of nucleic acids, protein folding, and cell deformation. Existing mainstream technologies include optical tweezers, magnetic tweezers, and atomic force microscopes, which have limitations such as high cost, low throughput, and complex operation. As an emerging technology, the centrifugal force microscopy dynamic simulation and observation device realizes high-throughput single-molecule experiments by combining centrifugal force fields with microscopic imaging. However, the current centrifugal force microscopy dynamic simulation and observation device has the following problems: it can only provide one-dimensional observations in a single axis and cannot analyze complex mechanical behaviors in two-dimensional space. In addition, the device assembly process is cumbersome, and components such as optical elements are not integrated into a closed space. The stability is low, sample loading is difficult, and the provision of centrifugal force depends on commercial centrifuges.

[0004] Therefore, the existing technology needs to be further developed. Summary of the Invention

[0005] The purpose of the present invention is to overcome the above technical deficiencies and provide a centrifugal force microscopic dynamic simulation and observation device to solve the problems existing in the prior art.

[0006] To achieve the above technical objectives, the present invention provides a centrifugal force microscopic dynamic simulation and observation device, comprising:

[0007] A centrifugal force module, used for providing centrifugal force;

[0008] A multidimensional microscope module is fixedly arranged on the centrifugal force module, and the multidimensional microscope module includes at least two microscope sub-modules for performing multidimensional dynamic observation of the sample to be measured;

[0009] The control module is in communication with both the centrifugal force module and the multi-dimensional microscope module, and is used to control the operation of the centrifugal force module and the multi-dimensional microscope module.

[0010] Specifically, the centrifugal force module includes a fixed base and a centrifugal assembly, the fixed base includes a fixed bottom plate and a fixed frame, the centrifugal assembly includes a centrifugal motor and a rotating rod, the rotating rod is connected to the rotating shaft of the centrifugal motor, and the centrifugal motor is fixed to the fixed bottom plate through the fixed frame;

[0011] The multidimensional microscope module is fixedly arranged on the rotating rod.

[0012] Specifically, the microscope submodule includes a housing, an optical component and a sleeve component. The optical component and the sleeve component are arranged inside the housing, and the optical component is fixedly connected to the sleeve component.

[0013] Specifically, the optical assembly includes a glass slide and a sample carrier, and the sleeve assembly includes a sample carrier sleeve. When in use, the glass slide is embedded in the sample carrier, and the sample carrier is fixedly arranged in the sample carrier sleeve.

[0014] Specifically, the sample carrier sleeve includes:

[0015] a cylindrical body, wherein the cylindrical body has a receiving cavity therein, and the receiving cavity is used for placing a slide glass;

[0016] At least two optical interfaces, the at least two optical interfaces being arranged through the side wall of the cylindrical body, the at least two optical interfaces being in communication with the accommodating cavity, and the at least two optical interfaces being distributed along the circumference of the cylindrical body;

[0017] The at least two optical interfaces are used to connect the microscope sub-modules in the multi-dimensional microscope module.

[0018] Specifically, when the multidimensional microscope module is a two-dimensional microscope module, the at least two microscope sub-modules include a first microscope sub-module and a second microscope sub-module, and the first microscope sub-module and the second microscope sub-module are arranged in mutually orthogonal directions to simultaneously perform dynamic observation of the sample to be tested in two-dimensional orthogonal directions.

[0019] Specifically, the first microscope submodule includes a first sleeve assembly, the second microscope submodule includes a second sleeve assembly, and the first sleeve assembly and the second sleeve assembly are vertically coupled to each other through a sample carrier sleeve.

[0020] Specifically, when the multidimensional microscope module is a three-dimensional microscope module, the at least two microscope sub-modules include a first microscope sub-module, a second microscope sub-module and a third microscope sub-module. The first microscope sub-module, the second microscope sub-module and the third microscope sub-module are orthogonal to each other in spatial orientation, forming a three-dimensional rectangular coordinate system for dynamic observation of the sample to be tested in three-dimensional orthogonal directions.

[0021] Specifically, the first microscope submodule includes a first sleeve assembly, the second microscope submodule includes a second sleeve assembly, and the third microscope submodule includes a third sleeve assembly;

[0022] The first sleeve assembly, the second sleeve assembly and the third sleeve assembly are vertically coupled via a sample carrier sleeve.

[0023] Specifically, the control module includes control software, a centrifugal motor control board and a WiFi module;

[0024] The centrifugal motor control board is used to control the speed and direction of the centrifugal motor, and the WiFi module is used to transmit the photos of the sample to be tested taken by the multi-dimensional microscope module to the control software.

[0025] Beneficial effects:

[0026] The present invention provides a centrifugal force microscopic dynamic simulation and observation device, comprising a centrifugal force module for providing centrifugal force and a multidimensional microscope module, including at least two microscope sub-modules, for performing multidimensional dynamic observation of a sample to be tested; a control module is used to control the operation of the centrifugal force module and the multidimensional microscope module, thereby solving the technical problems that traditional microscopic observation technology can only provide one-dimensional observation of a single axis in a centrifugal force field environment, cannot analyze complex deformation and mechanical behavior in a two-dimensional space, and optical elements and other components are not integrated into a closed space, resulting in low stability. The device greatly improves the observation dimension and data acquisition capability of the microscopic behavior of samples in a centrifugal force field, and realizes the synchronous capture of three-dimensional dynamic information of samples under centrifugal state through the spatial orthogonal layout of the multidimensional microscope module and the coordinated design of the optical interface of the sample carrier sleeve; at the same time, the intelligent integration of the control module significantly improves the experimental efficiency and accuracy, and greatly improves the intelligence, usability and reliability of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 1 is an overall structural diagram of a two-dimensional centrifugal force microscopic dynamic simulation and observation device provided in a specific embodiment of the present invention;

[0028] Figure 2 is a structural schematic diagram of a centrifugal force module provided in a specific embodiment of the present invention;

[0029] Figure 3 is a schematic structural diagram of a one-dimensional microscope module provided in a specific embodiment of the present invention;

[0030] Figure 4 is an exploded view of a one-dimensional microscope module provided in a specific embodiment of the present invention;

[0031] Figure 5 Schematic diagram of the structure of the sample carrier and the glass slide provided in a specific embodiment of the present invention;

[0032] Figure 6 Schematic diagram of the structure of the sample carrier sleeve provided in a specific embodiment of the present invention;

[0033] Figure 7 is a schematic structural diagram of a two-dimensional microscope module provided in a specific embodiment of the present invention;

[0034] Figure 8 is an exploded view of a two-dimensional microscope module provided in a specific embodiment of the present invention;

[0035] Figure 9 is a schematic diagram of the composition of a control module provided in a specific embodiment of the present invention;

[0036] Figure 10 1 is an overall structural diagram of a three-dimensional centrifugal force microscopic dynamic simulation and observation device provided in a specific embodiment of the present invention;

[0037] Figure 11 is a structural schematic diagram of a three-dimensional microscope module provided in a specific embodiment of the present invention;

[0038] Figure 12 is an exploded view of a three-dimensional microscope module provided in a specific embodiment of the present invention;

[0039] The reference numerals of the above drawings are as follows:

[0040] 1. Centrifugal force module; 2. 2D microscope module; 3. 3D microscope module; 11. Fixed base; 12. Centrifugal assembly; 13. Counterweight; 111. Fixed bottom plate; 112. Fixed frame; 121. Centrifugal motor; 122. Rotating rod; 123. Rotating shaft; 5. Housing; 6. Optical assembly; 7. Sleeve assembly; 8. Slide; 9. Sample carrier; 10. Sample carrier sleeve; 14. Cylindrical body; 15. Accommodating chamber; 16. Optical interface; 21. First housing; 22. Second housing; 23. Third housing; 61. Light source lamp holder; 62. Light source lamp; 63. Optical diffuser radiator; 64, Fresnel lens; 65, objective lens; 66, camera; 71, light source lamp sleeve; 72, optical diffuser sleeve; 73, Fresnel lens sleeve; 74, objective lens sleeve; 75, camera connecting sleeve; 611, first light source lamp holder; 621, first light source lamp; 631, first optical diffuser; 641, first Fresnel lens; 651, first objective lens; 661, first camera; 711, first light source lamp sleeve; 721, first optical diffuser sleeve; 731, first Fresnel lens sleeve; 741, first objective lens sleeve; 751, first camera connecting sleeve; 612, second light source lamp holder; 622, second light source lamp; 632, second optical diffuser; 642, second Fresnel lens; 652, second objective lens; 662, second camera; 712, second light source lamp sleeve; 722, second optical diffuser sleeve; 732, second Fresnel lens sleeve; 742, second objective lens sleeve; 752, second camera connecting sleeve; 613, third light source lamp holder; 623, third light source lamp; 633, third optical diffuser; 643, third Fresnel lens; 653, third objective lens; 663, third camera; 713, third light source lamp sleeve; 723, third optical diffuser sleeve; 733, third Fresnel lens sleeve; 743, third objective lens sleeve; 753, third camera connecting sleeve. DETAILED DESCRIPTION

[0041] In order to enable those skilled in the art to better understand the technical solution of the present invention, the technical solution of the present invention is clearly and completely described below in conjunction with the drawings of the present invention. Based on the embodiments in this application, other similar embodiments obtained by ordinary technicians in this field without making creative work should fall within the scope of protection of this application. In addition, the directional words mentioned in the following embodiments, such as "up", "down", "left", "right", etc., are only reference to the directions of the drawings. Therefore, the directional words used are used to illustrate rather than limit the invention.

[0042] The present invention will be further described below with reference to the accompanying drawings and preferred embodiments.

[0043] Example 1

[0044] See also Figure 1This embodiment provides a centrifugal force microscopic dynamic simulation and observation device, including a centrifugal force module 1, a multidimensional microscope module and a control module, wherein the centrifugal force module 1 is used to provide centrifugal force; the multidimensional microscope module is fixedly arranged on the centrifugal force module 1, and the multidimensional microscope module includes at least two microscope sub-modules for performing multi-dimensional dynamic observation of the sample to be tested; the control module is communicatively connected to both the centrifugal force module 1 and the multidimensional microscope module, and is used to control the operation and signal transmission of the centrifugal force module 1 and the multidimensional microscope module.

[0045] It can be understood that according to the above technical solution, the centrifugal force microscopy dynamic simulation and observation device provided in this embodiment solves the technical problems that traditional microscopy observation technology can only provide one-dimensional observation of a single axis in a centrifugal force field environment, cannot analyze complex deformation and mechanical behavior in two-dimensional space, and optical elements and other components are not integrated into a closed space, resulting in low stability, thereby realizing multi-dimensional dynamic observation of the sample to be tested under centrifugal state.

[0046] See also Figure 2 In this embodiment, the centrifugal force module 1 includes a fixed base 11 and a centrifugal assembly 12. The fixed base 11 includes a fixed bottom plate 111 and a fixed frame 112. The centrifugal assembly 12 includes a centrifugal motor 121 and a rotating rod 122. The rotating rod 122 is connected to the rotating shaft 123 of the centrifugal motor 121. The centrifugal motor 121 is placed on the fixed bottom plate 111 and fixed to the fixed bottom plate 111 through the fixed frame 112 to ensure that the centrifugal force microscopic dynamic simulation and observation device does not shake or sway during operation, ensuring that the entire device remains stable during high-speed rotation. The multidimensional microscope module is fixedly set on the rotating rod 122, so that when the rotating rod 122 rotates at high speed, it drives the multidimensional microscope to rotate together. According to the above technical solution, through the high-stability fixed structure and the design of fixing the multidimensional microscope to the centrifugal force module, in-situ, stable, multi-dimensional, and synchronized dynamic microscopic observation of the sample is achieved in a precisely controllable centrifugal force field.

[0047] Furthermore, the centrifugal motor 121 utilizes a high-speed or overspeed (>2000 rpm) motor to provide sufficient centrifugal force to deform the sample under test within the multidimensional microscope. Different centrifugal forces can be provided in two ways: first, by setting different rotational speeds to generate different centrifugal forces; second, by securing the multidimensional microscope module at different positions on the rotating shaft 122 to generate different centrifugal forces.

[0048] Furthermore, the rotating rod 122 can be fixed to the rotating shaft 123 of the centrifugal motor 121 by one of the following two fixing methods or a combination of the two methods:

[0049] Method 1: The rotating rod 122 is screwed onto the end of the rotating shaft 123 of the centrifugal motor 121 through a thread, and the upper and lower ends are fixed by two nuts;

[0050] Method 2: Screw the cross nut onto the end of the rotating shaft 123 of the centrifugal motor 121 , and at the same time, embed the cross into the cross groove of the rotating rod 122 and fix it to the rotating rod 122 through bolts.

[0051] Preferably, the fixed base plate 111 can be a heavier platform, such as an optical platform or other processed parts with a shock-absorbing effect, and the fixed frame 112 is made of aluminum profiles and aluminum profile angle pieces. The aluminum profile frame can provide strong support to resist deformation force. At the same time, the modularity of the aluminum profile and the precise interface characteristics of the optical platform significantly improve the ease of use, adjustability, scalability and installation accuracy of the device, laying a solid physical foundation for high-resolution, multi-dimensional microscopic dynamic observation under high-speed centrifugation state.

[0052] See also Figure 2 In some specific embodiments, the centrifugal module further includes a counterweight 13, which is fixedly mounted on the other side of the rotating rod 122 and symmetrically positioned with the multidimensional microscope module relative to the end of the centrifugal motor 121. The counterweight 13 is used to maintain the balance of the entire centrifugal force microscopic dynamic simulation and observation device, thereby ensuring the stability of the multidimensional microscope module during high-speed rotation. Furthermore, an identical multidimensional microscope module may be placed on the other side of the rotating rod 122, i.e., symmetrically with the multidimensional microscope module, to ensure the balance and stability of the multidimensional microscope module during high-speed rotation of the rotating rod 122.

[0053] See also Figure 3 In this embodiment, the microscope submodule includes a housing 5, an optical component 6 and a sleeve component 7. The optical component 6 and the sleeve component 7 are arranged inside the housing 5, and the optical component 6 and the sleeve component 7 are fixedly connected.

[0054] For details, see Figure 3 The housing 5 includes a first housing 21 and a second housing 22 , which together tightly enclose the optical component 6 and the sleeve component 7 .

[0055] For details, see Figure 4 The optical assembly 6 includes a glass slide 8 and a sample carrier 9, and the sleeve assembly 7 includes a sample carrier sleeve 10. When in use, the glass slide 8 is embedded in the sample carrier 9, and the sample carrier 9 is fixedly set in the sample carrier sleeve 10. This achieves in-situ, synchronous, multi-dimensional dynamic observation of the sample to be tested in the centrifugal field, ensuring that the observation process does not interfere with the natural state of the sample, and greatly improving the efficiency and reliability of multi-dimensional data acquisition.

[0056] For further information, see Figure 4 and Figure 5The optical component 6 also includes a light source lamp holder 61, a light source lamp 62, an optical diffuser 63, a Fresnel lens 64, an objective lens 65 and a camera 66. The sleeve assembly 7 also includes a light source lamp sleeve 71, an optical diffuser sleeve 72, a Fresnel lens sleeve 73, an objective lens sleeve 74 and a camera connecting sleeve 75. The light source lamp 62 is used to provide illumination light, the light source lamp holder 61 is used to install and fix the light source lamp 62, and the light source lamp 62 is installed (embedded) in the light source lamp holder 61. The optical diffuser 63 is located behind the light source and is used to uniformize the light emitted by the light source and reduce glare and hot spots. The optical diffuser 63 is fixedly arranged in the optical diffuser sleeve 72, and the Fresnel lens 64 is fixed in the Fresnel lens sleeve 73. The Fresnel lens 64 is usually used as a condenser to converge the diffused light to the sample area. Two slides 8 are embedded in the sample carrier 9, as shown. Figure 5 As shown, two glass slides are used to clamp the sample to be observed. The sample carrier 9 is a specially designed clamp or bracket for fixing the two glass slides 8 with the sample. The sample carrier 9 is fixed in the sample carrier sleeve 10 in an inserted manner, which is convenient for rapid installation and replacement of the sample. The objective lens 65 is fixed in the objective lens sleeve 74. The objective lens 65 is placed close to the sample and is responsible for collecting the light emitted by or transmitted through the sample and performing primary magnification imaging. The camera 66 is located at the end of the imaging light path (after the objective lens) and is used to receive the optical image formed by the objective lens and convert it into an electronic signal (image). The light source lamp holder 61 and all the sleeves are threadedly connected from left to right to form a one-dimensional microscope module.

[0057] See also Figure 6 In this embodiment, the sample carrier sleeve 10 includes a cylindrical body 14 and at least two optical interfaces 16, wherein the cylindrical body 14 has a receiving cavity 15 therein, and the receiving cavity 15 is used to place the slide 8; and the at least two optical interfaces 16 are arranged through the side wall of the cylindrical body 14, are connected to the receiving cavity 15, and are distributed along the circumference of the cylindrical body 14; wherein each optical interface 16 includes a light-transmitting area for allowing the light path of the corresponding connected microscope submodule to pass through and reach the sample to be measured in the receiving cavity 15;

[0058] Specifically, at least two optical interfaces 16 are used to connect the various microscope sub-modules in the multidimensional microscope module. The position of each optical interface 16 matches the observation light path of each microscope sub-module in the multidimensional microscope module to achieve dynamic observation of different orientations of the sample under rotation. According to the above technical solution, the sample carrier sleeve in this embodiment is designed with circumferentially distributed optical interfaces to achieve multi-angle light path penetration while ensuring high-speed rotation stability, allowing multiple microscope sub-modules to synchronously, in situ, and multi-dimensionally dynamically observe sample deformation in the centrifugal field.

[0059] See also Figure 7In this embodiment, the multidimensional microscope module is a two-dimensional microscope module 2, which includes a first microscope submodule and a second microscope submodule. The first microscope submodule and the second microscope submodule are identical and arranged in mutually orthogonal directions to simultaneously perform dynamic observation of the sample under test in two orthogonal directions. By orthogonally arranging two identical microscope submodules, the two-dimensional microscope module 2 of this embodiment synchronously acquires real-time deformation data of the sample in mutually perpendicular directions within a centrifugal force field, completely eliminating the observation angle deviation caused by rotation. This provides high precision for research on material stress distribution, biological cell deformation, and other issues, significantly improving observation efficiency and data reliability.

[0060] For details, see Figure 7 and Figure 8 The first microscope submodule includes a first sleeve assembly, and the second microscope submodule includes a second sleeve assembly. The first and second sleeve assemblies are vertically coupled via a sample carrier sleeve 10. According to the above technical solution, mechanical coupling ensures that the dual optical paths are strictly orthogonal, eliminating errors caused by independent adjustment and maintaining zero observation deviation during high-speed rotation. The first and second microscope submodules share the same sample carrier cavity, and the control module can simultaneously trigger the dual cameras to achieve millisecond-level synchronized exposure, capturing the instantaneous correlation state of orthogonal deformations, creating a confocal synchronized imaging environment, and ensuring that the two-dimensional dynamic data has temporal and spatial consistency.

[0061] It should be further explained that, in order to expand the observation dimension, on the basis of the one-dimensional microscope module, a set of identical microscope modules are arranged along the orthogonal direction to form a two-dimensional microscope module 2, such as Figure 7 As shown, in the two-dimensional microscope module 2, the housing 5 includes a first housing 21 and a second housing 22, which together tightly enclose the optical component 6 and the sleeve component 7. The optical component 6 includes a first optical component and a second optical component, as shown in FIG. Figure 8As shown, the first optical assembly includes a first light source lamp holder 611, a first light source lamp 621, a first optical diffuser 631, a first Fresnel lens 641, a glass slide 8, a sample carrier 9, a first objective lens 651 and a first camera 661; the second optical assembly includes a second light source lamp holder 612, a second light source lamp 622, a second optical diffuser 632, a second Fresnel lens 642, a glass slide 8, a sample carrier 9, a second objective lens 652 and a second camera 662; the sample carrier 9 forms an angle of 45° with the microscope submodule in the x direction and the microscope submodule in the y direction; the sleeve assembly 7 includes the second A sleeve assembly and a second sleeve assembly, wherein the first sleeve assembly includes a first light source lamp sleeve 711, a first optical diffuser sleeve 721, a first Fresnel lens sleeve 731, a sample carrier sleeve 10, a first objective lens sleeve 741, and a first camera connecting sleeve 751; and the second sleeve assembly includes a second light source lamp sleeve 712, a second optical diffuser sleeve 722, a second Fresnel lens sleeve 732, a sample carrier sleeve 10, a second objective lens sleeve 742, and a second camera connecting sleeve 752. The first sleeve assembly and the second sleeve assembly are vertically coupled via the sample carrier sleeve 10. The sample carrier sleeve 10 includes two optical interfaces 16, which are respectively connected to the first microscope submodule and the second microscope submodule, thereby realizing two-dimensional dynamic observation of the sample to be measured in a rotating state.

[0062] It should be noted here that the first microscope sub-module is the same as the second microscope sub-module, the first light source lamp 621 is embedded in the first light source lamp holder 611, the first optical diffuser 631 is fixed in the first optical diffuser sleeve 721, the first Fresnel lens 641 is fixed in the first Fresnel lens sleeve 731, the glass slide 8 is embedded in the sample carrier 9, the sample carrier 9 is inserted and fixed in the sample carrier sleeve 10, the first objective lens 651 is screwed into the first objective lens sleeve 741, the first light source lamp holder 611 and the sleeves of all the first microscope sub-modules are connected by threads in the x and y directions in sequence, and the connection method of the various components of the second microscope sub-module is consistent with the connection method of the various components of the first microscope sub-module.

[0063] It is understood that in this embodiment, for the two-dimensional microscope module, the sample carrier sleeve 10 includes a cylindrical body 14 and two optical interfaces 16, wherein the cylindrical body 14 has a receiving cavity 15 therein for placing the slide 8; the two optical interfaces 16 are arranged through the side wall of the cylindrical body 14, are connected to the receiving cavity 15, and are distributed along the circumference of the cylindrical body 14; each optical interface 16 includes a light-transmitting area for allowing the light path of the corresponding connected microscope sub-module to pass through and reach the sample to be measured in the receiving cavity 15; the two optical interfaces 16 are used to connect the first microscope sub-module and the second microscope sub-module in the two-dimensional microscope module. The positions of the two optical interfaces 16 match the observation light paths of the first microscope sub-module and the second microscope sub-module in the two-dimensional microscope module to achieve two-dimensional dynamic observation of the sample in a rotating state.

[0064] See also Figure 9 In this embodiment, the control module includes control software, a centrifuge motor control board, and a WiFi module. The centrifuge motor control board is communicatively connected to the centrifuge motor 121 and is used to control the speed and direction of the centrifuge motor 121. The WiFi module connects the camera to the control software and is used to wirelessly transmit images of the sample to be tested, taken by the two-dimensional microscope module 2, to the control software. According to the above technical solution, the control module uses wireless communication and an integrated control architecture to remotely and precisely control the speed and direction of the centrifuge in high-speed centrifugation scenarios and simultaneously transmit multi-dimensional microscopic images, completely eliminating the safety risks and data delays associated with manual intervention in traditional observations. The software platform also enables the spatiotemporal correlation of centrifuge parameters and dynamic deformation data, providing closed-loop analysis capabilities for material responses under complex force fields. Furthermore, the camera can use a built-in WiFi chip, eliminating the need for an additional WiFi module.

[0065] See also Figure 9 Furthermore, the controller module in this embodiment is intended to control the operation of the two-dimensional centrifugal force microscopy dynamic simulation and observation device in this embodiment and transmit image signals. In addition to the control software, the centrifugal motor control board and the WiFi module, the control module also includes a power supply and a boost module, wherein the power supply can be a lithium battery, and the boost module adjusts the output voltage of the power supply to the rated voltage value of the WiFi module, the light source lamp holder 61 and the camera 66. The light source lamp holder 61 can be an LED lamp holder, thereby realizing the power supply of the two-dimensional microscope module 2.

[0066] The following is a specific example to illustrate the specific implementation process of the centrifugal force microscopic dynamic simulation and observation device in this embodiment:

[0067] (1) Sample preparation: The operator prepares two slides 8, such as Figure 5As shown, a glass slide 8 is first inserted into a single-sided projection on a sample carrier 9 and taped to the side of the carrier 9. For solid samples, the sample is cut into thin pieces and placed on slide 8. For liquid samples, a pipette, such as a pipette, is used to drop the sample onto slide 8. Another glass slide 8 is then inserted into the other side projection of the sample carrier 9 and taped to the side of the carrier 9. This places the sample in the gap between the two slides 8. For samples of single molecules (e.g., DNA / protein) and micro / nanostructures (e.g., DNA nanomachines), streptavidin and magnetic microbeads are used. Streptavidin binds the sample to slide 8, while the magnetic microbeads break the sample under centrifugal force, facilitating mechanical property analysis.

[0068] (2) The sample carrier 9 is positioned: the sample carrier 9 is inserted into the accommodating cavity 15 of the sample carrier sleeve 10;

[0069] (3) Replacement of the counterweight 13: Select the corresponding counterweight 13 according to the multidimensional microscope module used (two-dimensional microscope module 2 or three-dimensional microscope module 3) to ensure that both ends of the rotating rod 122 are balanced;

[0070] (3) Instrument trial operation: Turn on the power of the centrifugal motor 121 and the two-dimensional microscope module 2 (or three-dimensional microscope module 3) to ensure that the images can be normally transmitted to the computer (control software);

[0071] (4) Instrument operation: The centrifugal motor 121 speed is adjusted through the centrifugal motor control board and the motor is operated. The rotation of the motor drives the two-dimensional microscope module 2 (or the three-dimensional microscope module 3) to rotate, and the camera 66 takes pictures of the sample in real time and transmits them back to the computer through the WiFi module;

[0072] (5) Image acquisition: The image is acquired through computer control software, and the image covers the process of the sample from the initial state to the disappearance state;

[0073] (6) Data analysis: Collating the collected images, analyzing the time when the sample is deformed or dissociated due to the centrifugal force and the magnitude of the centrifugal force, and repeating the operation steps to observe and analyze the effects of different centrifugal forces on the sample by adjusting the speed of the centrifugal motor 121 or the fixed position of the two-dimensional microscope module 2 (or the three-dimensional microscope module 3);

[0074] (7) After the experiment, the operator removes the sample and turns off the instrument power.

[0075] It should be noted here that this embodiment provides a two-dimensional centrifugal force microscopic dynamic simulation and observation device, including a centrifugal force module for providing centrifugal force and a two-dimensional microscope module, for performing multi-dimensional dynamic observation of the sample to be tested; the control module is used to control the operation of the centrifugal force module 1 and the two-dimensional microscope module, which solves the technical problems that traditional microscopic observation technology can only provide one-dimensional observation of a single axis in a centrifugal force field environment, cannot analyze the complex deformation and mechanical behavior in the two-dimensional space, and optical elements and other components are not integrated into a closed space, resulting in low stability. It greatly improves the observation dimension and data acquisition capability of the microscopic behavior of samples in complex force fields, and realizes the synchronous capture of the two-dimensional dynamic information of the sample under the centrifugal state through the orthogonal layout of the two-dimensional microscope module and the coordinated design of the optical interface of the sample carrier sleeve; at the same time, the intelligent integration of the control module significantly improves the experimental efficiency and accuracy, and greatly improves the intelligence, usability and reliability of the present invention.

[0076] Example 2

[0077] See also Figure 10 This embodiment provides a three-dimensional centrifugal force microscopic dynamic simulation and observation device. Unlike the above-mentioned embodiment 1, the multidimensional microscope module in this embodiment is a three-dimensional microscope module 3. The three-dimensional centrifugal force microscopic dynamic simulation and observation device includes a centrifugal force module 1, a three-dimensional microscope module 3 and a control module, wherein the centrifugal force module 1 is used to provide centrifugal force, the three-dimensional microscope module 3 is fixedly arranged on the centrifugal force module 1, the three-dimensional microscope module 3 includes a first microscope sub-module, a second microscope sub-module and a third microscope sub-module, and the three-dimensional microscope module 3 is used to perform three-dimensional dynamic observation of the sample to be tested; and the control module is communicated with the centrifugal force module 1 and the three-dimensional microscope module 3, and is used to control the operation of the centrifugal force module 1 and the multidimensional microscope module. According to the above technical solution, it is achieved to synchronously capture the three-dimensional spatial deformation field of the sample in a high-speed rotating coordinate system, breaking through the planar limitations of traditional centrifugal observations and revealing the full-dimensional dynamic laws of material volume deformation and biological cell three-dimensional structure evolution under the action of centrifugal force.

[0078] See also Figure 6 In the three-dimensional centrifugal force microscopic dynamic simulation and observation device of this embodiment, the sample carrier sleeve 10 includes a cylindrical body 14, which has a receiving cavity 15 therein, and the receiving cavity 15 is used to place the slide 8; the sample carrier sleeve 10 also includes three optical interfaces 16, which are arranged through the side wall of the cylindrical body 14 and are connected to the receiving cavity 15. The three optical interfaces 16 are distributed along the circumference of the cylindrical body 14; each optical interface 16 includes a light-transmitting area for allowing the light path of the corresponding connected microscope submodule to pass through and reach the sample to be measured in the receiving cavity 15;

[0079] Specifically, the three optical interfaces 16 are respectively used to connect the first microscope sub-module, the second microscope sub-module and the third microscope sub-module in the three-dimensional microscope module 3. The position of each optical interface 16 matches the observation light path of each microscope sub-module in the three-dimensional microscope module 3 to realize three-dimensional dynamic observation of the sample to be measured in a rotating state.

[0080] Furthermore, in the three-dimensional centrifugal force microscopic dynamic simulation and observation device of this embodiment, the first microscope sub-module, the second microscope sub-module and the third microscope sub-module are orthogonal to each other in spatial orientation, forming a three-dimensional rectangular coordinate system to perform dynamic observation of the sample to be tested in three-dimensional orthogonal directions.

[0081] For details, see Figure 11 and Figure 12 The first microscope submodule includes a first sleeve assembly, the second microscope submodule includes a second sleeve assembly, and the third microscope submodule includes a third sleeve assembly. The first sleeve assembly, the second sleeve assembly, and the third sleeve assembly are vertically coupled via a sample carrier sleeve 10. According to the above technical solution, the three-dimensional orthogonal coupling design of the above-mentioned three-dimensional microscope module uses a three-way mechanical interlocking of the sample carrier sleeve 10 to forcibly constrain the spatial orthogonality of the three optical paths with a rigid structure, and simultaneously constructs a capture system for the three-dimensional dynamic deformation field in the rotating coordinate system, thereby realizing three-dimensional dynamic observation of the sample to be measured under centrifugal force.

[0082] It should be further explained that, in order to expand the observation dimension, based on the first microscope sub-module, two sets of identical microscope modules, namely the second microscope sub-module and the third microscope sub-module, are arranged along the two orthogonal directions of x and y, thereby forming a three-dimensional microscope module 3.

[0083] See also Figure 11 In this embodiment of the 3D centrifugal force microscopy dynamic simulation and observation device, the 3D microscope module 3 includes a housing 5, an optical component assembly, and a sleeve assembly 7 to meet the requirements of 3D observation. The housing 5 comprises a first housing 21, a second housing 22, and a third housing 23, which together tightly enclose the optical assembly 6 and sleeve assembly 7.

[0084] See also Figure 12In the three-dimensional centrifugal force microscopy dynamic simulation and observation device of this embodiment, the optical element assembly includes a first optical assembly, a second optical assembly and a third optical assembly. The first optical assembly includes a first light source lamp holder 611, a first light source lamp 621, a first optical diffuser 631, a first Fresnel lens 641, a glass slide 8, a sample carrier 9, a first objective lens 651 and a first camera 661; the second optical assembly includes a second light source lamp holder 612, a second light source lamp 622, a second optical diffuser 632, a second Fresnel lens 642, a glass slide 8, a sample carrier 9, a second objective lens 652 and a second camera 662; the third optical assembly includes a third light source lamp holder 613, a third light source lamp 623, a third optical diffuser 633, a third Fresnel lens 643, a glass slide 8, a sample carrier 9, a third objective lens 653 and a third camera 663. The sample carrier 9 is at a 45° angle to the microscope module in the x-direction and the microscope module in the y-direction.

[0085] See also Figure 12 In the three-dimensional centrifugal force microscopic dynamic simulation and observation device of this embodiment, the sleeve assembly 7 includes a first sleeve assembly, a second sleeve assembly and a third sleeve assembly. The first sleeve assembly includes a first light source lamp sleeve 711, a first optical diffuser sleeve 721, a first Fresnel lens sleeve 731, a sample carrier sleeve 10, a first objective lens sleeve 741 and a first camera connecting sleeve 751. The second sleeve assembly includes a second light source lamp sleeve 712, a second optical diffuser sleeve 721, a first Fresnel lens sleeve 731, a sample carrier sleeve 10, a first objective lens sleeve 741 and a first camera connecting sleeve 751. 2. The second Fresnel lens sleeve 732, the sample carrier sleeve 10, the second objective lens sleeve 742 and the second camera connecting sleeve 752. The third sleeve assembly includes a third light source lamp sleeve 713, a third optical diffuser sleeve 723, a third Fresnel lens sleeve 733, the sample carrier sleeve 10, the third objective lens sleeve 743 and the third camera connecting sleeve 753. The first sleeve assembly, the second sleeve assembly and the third sleeve assembly are vertically coupled and connected through the sample carrier sleeve 10. Among them, the first light source lamp 621 is embedded in the first light source lamp holder 611, the first optical diffuser 631 is fixed in the first optical diffuser sleeve 721, the first Fresnel lens 641 is fixed in the first Fresnel lens sleeve 731, the glass slide 8 is embedded in the sample carrier 9, the sample carrier 9 is inserted and fixed in the sample carrier sleeve 10, the first objective lens 651 is screwed tight in the first objective lens sleeve 741, the first light source lamp holder 611 and all the sleeves of the three-dimensional microscope module 3 are threadedly connected in the x direction, y direction and z direction in sequence, and the connection method of each component in the second microscope sub-module and the third microscope sub-module is consistent with the connection method of each component in the first microscope sub-module, thereby realizing three-dimensional dynamic observation of the sample to be tested on the glass slide 8 in the sample carrier sleeve 10.

[0086] See also Figure 9In this embodiment, the control module includes control software, a centrifugal motor control board and a WiFi module. The centrifugal motor control board is communicatively connected to the centrifugal motor 121. The centrifugal motor control board is used to control the speed and direction of the centrifugal motor 121. The WiFi module is communicatively connected to the control software. The WiFi module is used to wirelessly transmit the photos of the sample to be tested taken by the three-dimensional microscope module 3 to the control software.

[0087] See also Figure 9 Furthermore, the controller module in this embodiment is intended to control the operation of the three-dimensional centrifugal force microscopy dynamic simulation and observation device in this embodiment and transmit image signals. In addition to the control software, the centrifugal motor control board and the WiFi module, the control module also includes a power supply and a boost module, wherein the boost module adjusts the output voltage of the power supply to the rated voltage value of the WiFi module, the light source lamp holder 61 and the camera 66, thereby realizing the power supply of the three-dimensional microscope module 3.

[0088] It should be noted here that this embodiment provides a three-dimensional centrifugal force microscopic dynamic simulation and observation device, including a centrifugal force module for providing centrifugal force and a three-dimensional microscope module, for performing multi-dimensional dynamic observation of the sample to be tested; the control module is used to control the operation of the centrifugal force module and the three-dimensional microscope module, which solves the technical problems that traditional microscopic observation technology can only provide one-dimensional observation of a single axis in a centrifugal force field environment, cannot analyze complex mechanical behaviors in two-dimensional space, and optical elements and other components are not integrated into a closed space, resulting in low stability. It greatly improves the observation dimension and data acquisition capability of the microscopic behavior of samples in complex force fields, and realizes the synchronous capture of three-dimensional dynamic information of samples under centrifugal state through the spatial orthogonal layout of the three-dimensional microscope module and the coordinated design of the optical interface of the sample carrier sleeve; at the same time, the intelligent integration of the control module significantly improves the experimental efficiency and accuracy, and greatly improves the intelligence, usability and reliability of the present invention.

[0089] It should be noted that the terms "first", "second", etc. in the specification and claims of the present application and the above-mentioned drawings are used to distinguish similar objects and are not necessarily used to describe a specific order or sequential order. It should be understood that the data used in this way can be interchangeable where appropriate, so that the embodiments of the present application described herein can be implemented in a sequence other than those illustrated or described herein. In addition, the terms "including" and "having" and any of their variations are intended to cover non-exclusive inclusions, for example, a process, method, system, product or device comprising a series of steps or units is not necessarily limited to those steps or units clearly listed, but may include other steps or units that are not clearly listed or inherent to these processes, methods, products or devices.

[0090] The various technical features described above can be combined arbitrarily. Although not all possible combinations of these technical features are described, any combination of these technical features should be considered to be covered by this specification as long as such combination does not conflict.

[0091] The specific embodiments of the present invention described above do not limit the scope of protection of the present invention. Any other corresponding changes and modifications made based on the technical concept of the present invention should be included in the scope of protection of the claims of the present invention.

Claims

1. A centrifugal force microscopic dynamic simulation and observation device, characterized in that: include: A centrifugal force module (1), configured to provide centrifugal force; A multidimensional microscope module, fixedly arranged on the centrifugal force module (1), the multidimensional microscope module comprising at least two microscope submodules, for performing multidimensional dynamic observation of a sample to be measured; a control module, in communication with both the centrifugal force module (1) and the multidimensional microscope module, and configured to control the operation of the centrifugal force module (1) and the multidimensional microscope module; The microscope submodule includes a sleeve assembly (7); The sleeve assembly (7) includes a sample carrier sleeve (10); The sample carrier sleeve (10) comprises: A cylindrical body (14), wherein the cylindrical body (14) has a receiving cavity (15); At least two optical interfaces (16), the at least two optical interfaces (16) being arranged through the side wall of the cylindrical body (14), the at least two optical interfaces (16) being in communication with the accommodating cavity (15), and the at least two optical interfaces (16) being distributed along the circumference of the cylindrical body (14); The at least two optical interfaces (16) are used to connect the microscope submodules in the multidimensional microscope module; The microscope submodule further comprises a housing (5) and an optical assembly (6), wherein the optical assembly (6) and a sleeve assembly (7) are arranged inside the housing (5), and the optical assembly (6) is fixedly connected to the sleeve assembly (7); The optical assembly (6) comprises a glass slide (8) and a sample carrier (9). When in use, the glass slide (8) is embedded in the sample carrier (9), and the sample carrier (9) is fixedly arranged in the sample carrier sleeve (10); The centrifugal force module (1) comprises a centrifugal assembly (12), the centrifugal assembly (12) comprises a rotating rod (122), and the multidimensional microscope module is fixedly arranged on the rotating rod (122).

2. The centrifugal force microscopic dynamic simulation and observation device according to claim 1, characterized in that: The centrifugal force module (1) further comprises a fixed base (11), the fixed base (11) comprising a fixed bottom plate (111) and a fixed frame (112), the centrifugal assembly (12) further comprises a centrifugal motor (121), the rotating rod (122) is connected to a rotating shaft (123) of the centrifugal motor (121), and the centrifugal motor (121) is fixed to the fixed bottom plate (111) via the fixed frame (112).

3. The centrifugal force microscopic dynamic simulation and observation device according to claim 1, characterized in that: The accommodating cavity (15) is used for placing a glass slide (8).

4. The centrifugal force microscopic dynamic simulation and observation device according to claim 3, characterized in that: When the multidimensional microscope module is a two-dimensional microscope module (2), the at least two microscope submodules include a first microscope submodule and a second microscope submodule, and the first microscope submodule and the second microscope submodule are arranged in mutually orthogonal directions to simultaneously perform dynamic observation of the sample to be measured in two-dimensional orthogonal directions.

5. The centrifugal force microscopic dynamic simulation and observation device according to claim 4, characterized in that: The first microscope submodule includes a first sleeve assembly, the second microscope submodule includes a second sleeve assembly, and the first sleeve assembly and the second sleeve assembly are vertically coupled to each other via a sample carrier sleeve (10).

6. The centrifugal force microscopic dynamic simulation and observation device according to claim 3, characterized in that: When the multidimensional microscope module is a three-dimensional microscope module (3), the at least two microscope submodules include a first microscope submodule, a second microscope submodule and a third microscope submodule, and the first microscope submodule, the second microscope submodule and the third microscope submodule are orthogonal to each other in spatial orientation, forming a three-dimensional rectangular coordinate system to perform dynamic observation of the sample to be measured in three-dimensional orthogonal directions.

7. The centrifugal force microscopic dynamic simulation and observation device according to claim 6, characterized in that: The first microscope submodule includes a first sleeve assembly, the second microscope submodule includes a second sleeve assembly, and the third microscope submodule includes a third sleeve assembly; The first sleeve assembly, the second sleeve assembly and the third sleeve assembly are vertically coupled via a sample carrier sleeve (10).

8. The centrifugal force microscopic dynamic simulation and observation device according to claim 2, characterized in that: The control module includes control software, a centrifugal motor control board and a WiFi module; The centrifugal motor control board is used to control the rotation speed and direction of the centrifugal motor (121), and the WiFi module is used to transmit the photos of the sample to be tested taken by the multi-dimensional microscope module to the control software.

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