Composite seaweed fertilizer and application thereof

Abstract

The invention relates to a compound seaweed fertilizer and application thereof, and belongs to the technical field of agricultural chemicals. According to the invention, the pyridine ester compound and the seaweed fertilizer are compounded for use, and the pyridine ester compound and the seaweed fertilizer have a synergistic effect, so that the growth of cucumbers and the quality of fruits are remarkably improved. The compound seaweed fertilizer disclosed by the invention can be used for remarkably improving the yield, the length, the transverse diameter and the thickness of cucumbers and the contents of protein, total sugar, fat, vitamin B2, vitamin C, iron element and calcium element of the cucumbers.

Description

Technical Field

[0001] The invention relates to a composite seaweed fertilizer and application thereof, belonging to the technical field of agricultural chemicals. Background Art

[0002] Seaweed fertilizer, an organic fertilizer made from natural seaweed, contains trace elements such as iodine, manganese, and titanium, as well as seaweed polysaccharides, which are rarely found in terrestrial plants. It also contains a variety of plant-derived endogenous hormones, including auxin, cytokinin, gibberellin, abscisic acid, and ethylene. These endogenous hormones can promote plant growth and increase yields.

[0003] Cucumber, also known as cucumber or green melon, is an important vegetable crop. Its stems and branches are elongated, grooved, and covered with white, coarse hairs. The tendrils are thin, and the petioles are slightly rough and hairy. The leaves are broadly ovate-cordate, membranous, with triangular, serrated lobes. The fruit is oblong or cylindrical, yellow-green when ripe, and has a rough surface. The seeds are small, narrowly ovate, white, without margins, and with nearly acute ends. Cucumbers have considerable edible and medicinal value.

[0004] With the improvement of living standards, people's requirements for the quality of vegetable products are also increasing. They not only require beautiful appearance and good flavor, but also require them to have rich and balanced nutrients and high health value. Although the combination of seaweed fertilizer and conventional plant growth regulators can increase plant yield and improve plant quality to a certain extent, the improvement is limited. Summary of the Invention

[0005] The purpose of the present invention is to provide a composite seaweed fertilizer and application thereof, so as to improve the yield and fruit quality of cucumbers.

[0006] The present invention provides a composite seaweed fertilizer, comprising a pyridine ester compound and seaweed fertilizer, wherein the mass of the pyridine ester compound is 1.5-2.5% of the mass of the seaweed fertilizer; the structure of the pyridine ester compound is as follows:

[0007]

[0008] Wherein, R is a C1-C3 alkylene group;

[0009] The preparation method of the seaweed fertilizer is as follows: mixing Ascophyllum nodosum, kelp, pomelo peel and water and then crushing them to obtain a crushed liquid; then enzymatically hydrolyzing the crushed liquid to obtain the seaweed fertilizer; the mass ratio of Ascophyllum nodosum, kelp and pomelo peel is 3-4:3-4:1-1.5.

[0010] Preferably, the R is ethylene.

[0011] Preferably, the pyridine ester compound is prepared by reacting 2-propanol pyridine and an acyl chloride compound, the acyl chloride compound is 3-chloropropionyl chloride, 4-chlorobutyryl chloride or chloroacetyl chloride, and the molar ratio of the 2-propanol pyridine to the acyl chloride compound is 1:1.1-1.2.

[0012] Preferably, the mass ratio of the ascophyllum nodosum, kelp, grapefruit peel and water is 3-4:3-4:1-1.5:10; the complex enzyme used in the enzymatic hydrolysis is composed of protease, pectinase, oxidoreductase and cellulase in a mass ratio of 20:8:15:35; the mass ratio of the crushing liquid and the complex enzyme is 100:1, the pH during enzymatic hydrolysis is 6.5-7.5, the temperature is 35-40°C, and the time is 70-80 hours.

[0013] An application of the composite seaweed fertilizer as described above.

[0014] Preferably, the application includes the following steps: spraying the pyridine ester compound and the seaweed fertilizer in the composite seaweed fertilizer on the leaves of the plants in the form of diluted solutions respectively.

[0015] Preferably, the plant is cucumber; the spraying is carried out after the cucumber is planted and the seedlings have grown, and the seaweed fertilizer dilution is sprayed once every 15 to 17 g, with a spraying amount of 600 to 700 kg / hm 2 18-20 days after cucumbers are transplanted and seedlings are hardened, spray the diluted solution of pyridine ester compounds once, with a spraying rate of 600-700 kg / hm2. 2 The seaweed fertilizer dilution liquid is prepared by mixing the seaweed fertilizer and water in a mass ratio of 1-1.5 g:1.5 kg, and the pyridine ester compound dilution liquid is prepared by mixing the pyridine ester compound and water in a mass ratio of 0.0015-0.0025 g:1.5 kg.

[0016] Preferably, the application is to increase the yield of cucumber.

[0017] Preferably, the application is to increase the length, transverse diameter and flesh thickness of cucumbers.

[0018] Preferably, the application is to increase the content of protein, total sugar, fat, vitamin B2, vitamin C, iron and calcium in cucumbers.

[0019] Compared to existing technologies, the present invention has the following beneficial effects: by combining pyridinium ester compounds with seaweed fertilizer, the two have a synergistic effect, significantly improving the growth and fruit quality of cucumbers. The composite seaweed fertilizer of the present invention can significantly increase cucumber yield, length, diameter, flesh thickness, and protein, total sugar, fat, vitamin B2, vitamin C, iron, and calcium content. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 This is the H NMR spectrum of the pyridine ester compound prepared in Example 1 of the present invention. DETAILED DESCRIPTION

[0021] The following examples are intended to further illustrate the present invention, but are not intended to limit the scope of protection of the present invention.

[0022] Example 1

[0023] The composite seaweed fertilizer of this embodiment includes a pyridine ester compound and seaweed fertilizer, wherein the mass of the pyridine ester compound is 1.5% of the mass of the seaweed fertilizer. The preparation method of the seaweed fertilizer is as follows: Ascophyllum nodosum, kelp, grapefruit peel and water are stirred and mixed in a mass ratio of 3:4:1:10, and then crushed to obtain a crushed liquid; then the crushed liquid and a composite enzyme (the composite enzyme consists of protease, pectinase, oxidoreductase and cellulase in a mass ratio of 20:8:15:35, and the protease, pectinase, oxidoreductase and cellulase are all commercially available) are added to a stirring tank in a mass ratio of 100:1 for enzymolysis, wherein the pH during enzymolysis is 6.5, the temperature is 35°C, and the time is 70 hours. After the enzymolysis is completed, the seaweed fertilizer is obtained.

[0024] The preparation method of pyridine ester compounds is as follows: add 2-propanol pyridine and dichloromethane to a reactor, stir evenly to obtain a 20% mass fraction of 2-propanol pyridine solution, then adjust the temperature of the material in the reactor to -7°C, start stirring, add the acyl chloride compound to the reactor, after the addition is complete, add triethylamine to the reactor, and then adjust the temperature of the material in the reactor to room temperature, continue stirring and reacting for 5 hours, then add 20% mass fraction of ammonia water to the reactor, stir for 30 minutes, let stand and separate, evaporate the organic phase to obtain a concentrated solution, and then purify the concentrated solution by column chromatography (the eluent used for column chromatography purification is a mixed solvent of ethyl acetate and petroleum ether with a volume ratio of 3:7) to obtain a pyridine ester compound; wherein the acyl chloride compound is 3-chloropropionyl chloride, and the molar ratio of N element in 2-propanol pyridine, acyl chloride compound and ammonia water is 1:1.1:1.4. The nuclear magnetic hydrogen spectrum of the pyridine ester compound prepared in this embodiment is shown as follows Figure 1 The chemical structure is shown below:

[0025]

[0026] Example 2

[0027] The composite seaweed fertilizer of this embodiment includes a pyridine ester compound and seaweed fertilizer, wherein the mass of the pyridine ester compound is 2.0% of the mass of the seaweed fertilizer; wherein the preparation method of the seaweed fertilizer is as follows: Ascophyllum nodosum, kelp, grapefruit peel and water are stirred and mixed in a mass ratio of 4:3:1.2:10, and then crushed to obtain a crushed liquid; then the crushed liquid and a composite enzyme (the composite enzyme is composed of protease, pectinase, oxidoreductase and cellulase in a mass ratio of 20:8:15:35, and the protease, pectinase, oxidoreductase and cellulase are all commercially available) are added to a stirring tank in a mass ratio of 100:1 for enzymolysis, wherein the pH during enzymolysis is 7, the temperature is 37°C, and the time is 75 hours. After the enzymolysis is completed, the seaweed fertilizer is obtained.

[0028] The preparation method of pyridine ester compounds is as follows: 2-propanol pyridine and dichloromethane are added to a reactor and stirred evenly to obtain a 25% mass fraction of 2-propanol pyridine solution, and then the temperature of the material in the reactor is adjusted to -4 ° C, stirring is started, and the acyl chloride compound is added dropwise to the reactor. After the dropwise addition is completed, triethylamine is added to the reactor, and the temperature of the material in the reactor is adjusted to room temperature. The stirring reaction is continued for 6 hours, and then 20% mass fraction of ammonia water is added to the reactor, stirred for 40 minutes, and allowed to stand for stratification. The organic phase is rotary evaporated to obtain a concentrated solution, and the concentrated solution is purified by column chromatography (the eluent used for column chromatography purification is a mixed solvent of ethyl acetate and petroleum ether with a volume ratio of 3:7) to obtain a pyridine ester compound; wherein the acyl chloride compound is 3-chloropropionyl chloride, and the molar ratio of the N element in 2-propanol pyridine, the acyl chloride compound and the ammonia water is 1:1.1:1.5. The chemical structure of the pyridine ester compound prepared in this embodiment is shown below:

[0029]

[0030] Example 3

[0031] The composite seaweed fertilizer of this embodiment includes a pyridine ester compound and seaweed fertilizer, wherein the mass of the pyridine ester compound is 2.5% of the mass of the seaweed fertilizer; wherein the preparation method of the seaweed fertilizer is as follows: Ascophyllum nodosum, kelp, grapefruit peel and water are stirred and mixed in a mass ratio of 4:4:1.5:10, and then crushed to obtain a crushed liquid; then the crushed liquid and a composite enzyme (the composite enzyme consists of protease, pectinase, oxidoreductase and cellulase in a mass ratio of 20:8:15:35, and the protease, pectinase, oxidoreductase and cellulase are all commercially available) are added to a stirring tank in a mass ratio of 100:1 for enzymolysis, wherein the pH during enzymolysis is 7.5, the temperature is 40°C, and the time is 80 hours. After the enzymolysis is completed, the seaweed fertilizer is obtained.

[0032] The preparation method of pyridine ester compounds is as follows: 2-propanol pyridine and dichloromethane are added to a reactor and stirred evenly to obtain a 2-propanol pyridine solution with a mass fraction of 30%, and then the temperature of the material in the reactor is adjusted to 0°C, stirring is started, and the acyl chloride compound is added dropwise to the reactor. After the dropwise addition is completed, triethylamine is added to the reactor, and the temperature of the material in the reactor is adjusted to room temperature. The stirring reaction is continued for 8 hours, and then 20% ammonia water is added to the reactor, stirred for 45 minutes, and allowed to stand for stratification. The organic phase is rotary evaporated to obtain a concentrated solution, and the concentrated solution is purified by column chromatography (the eluent used for column chromatography purification is a mixed solvent of ethyl acetate and petroleum ether with a volume ratio of 3:7) to obtain a pyridine ester compound; wherein the acyl chloride compound is 3-chloropropionyl chloride, and the molar ratio of the N element in 2-propanol pyridine, the acyl chloride compound and the ammonia water is 1:1.2:1.5. The chemical structure of the pyridine ester compound prepared in this embodiment is shown below:

[0033]

[0034] Example 4

[0035] The only difference between the composite seaweed fertilizer of this embodiment and the composite seaweed fertilizer of Example 1 is that the acyl chloride compound in the preparation method of the pyridine ester compound in the composite seaweed fertilizer of this embodiment is 4-chlorobutyryl chloride.

[0036] Example 5

[0037] The only difference between the composite seaweed fertilizer of this embodiment and the composite seaweed fertilizer of Example 1 is that the acyl chloride compound in the preparation method of the pyridine ester compound in the composite seaweed fertilizer of this embodiment is chloroacetyl chloride.

[0038] Comparative Example 1

[0039] The difference between the composite seaweed fertilizer of this comparative example and the composite seaweed fertilizer of Example 1 is that the pyridine ester compound is replaced by diethylaminoethyl hexanoate in the composite seaweed fertilizer of this comparative example.

[0040] Comparative Example 2

[0041] The only difference between the composite seaweed fertilizer of this comparative example and the composite seaweed fertilizer of Example 1 is that the acyl chloride compound in the preparation method of the pyridine ester compound in the composite seaweed fertilizer of this comparative example is propionyl chloride.

[0042] Comparative Example 3

[0043] The only difference between the composite seaweed fertilizer of this comparative example and the composite seaweed fertilizer of Example 1 is that the acyl chloride compound in the preparation method of the pyridine ester compound in the composite seaweed fertilizer of this comparative example is butyryl chloride.

[0044] Comparative Example 4

[0045] The difference between the composite seaweed fertilizer of this comparative example and the composite seaweed fertilizer of Example 1 is that the preparation method of the composite seaweed fertilizer of this comparative example is as follows: Ascophyllum nodosum, Laminaria japonica and water are stirred and mixed in a mass ratio of 3.5:4.5:10 and then crushed to obtain a crushed liquid; then the crushed liquid and a composite enzyme (the composite enzyme consists of protease, pectinase, oxidoreductase and cellulase in a mass ratio of 20:8:15:35, and the protease, pectinase, oxidoreductase and cellulase are all commercially available) are added into a stirring tank in a mass ratio of 100:1 for enzymolysis, the pH during enzymolysis is 6.5, the temperature is 35°C, and the time is 70 h. After the enzymolysis is completed, the seaweed fertilizer is obtained.

[0046] Comparative Example 5

[0047] The difference between the composite seaweed fertilizer of this comparative example and the composite seaweed fertilizer of Example 1 is that the preparation method of the composite seaweed fertilizer of this comparative example is as follows: grapefruit peel and water are stirred and mixed in a mass ratio of 8:10 and then crushed to obtain a crushed liquid; then the crushed liquid and a composite enzyme (the composite enzyme consists of protease, pectinase, oxidoreductase and cellulase in a mass ratio of 20:8:15:35, and the protease, pectinase, oxidoreductase and cellulase are all commercially available) are added to a stirring tank in a mass ratio of 100:1 for enzymolysis, the pH during enzymolysis is 6.5, the temperature is 35°C, and the time is 70 hours. After the enzymolysis is completed, seaweed fertilizer is obtained.

[0048] Experimental example

[0049] This experimental example is used to evaluate the effects of the composite seaweed fertilizers of various embodiments and comparative examples on cucumber yield, fruit appearance, and nutritional components in the fruit.

[0050] The experiment was conducted in a high-yield experimental field. The soil fertility parameters were as follows: organic matter 15.11g / kg, alkaline nitrogen 45.83mg / kg, available phosphorus (P2O5) 55.21mg / kg, and available potassium (K2O) 50.46mg / kg. The Yuexiu No. 1 cucumber variety was selected for the experiment. Before the experiment began, base fertilizer was applied to the experimental field. A total of 10 treatments were carried out. For each treatment, 3 plots were randomly selected from the experimental field for testing. Each plot had an area of 20m 2 , and set up protection rows, and each community is managed in the same way.

[0051] Treatment 1 is experimental group 1. Treatment method: starting from the time when cucumbers are planted and seedlings have been established, the seaweed fertilizer dilution in the composite seaweed fertilizer of Example 1 is applied once every 15 days (the seaweed fertilizer dilution is prepared by mixing 1 g of the composite seaweed fertilizer of Example 1 with 1.5 kg of water), for a total of 3 applications, with each application amount being 600 kg / hm2. 2The application method is to spray on the cucumber leaves; and on the 20th day after the cucumbers are planted and the seedlings are slowly grown, the pyridine ester compound dilution in the composite seaweed fertilizer of Example 1 is applied once (the pyridine ester compound dilution is prepared by uniformly stirring 0.015g of the pyridine ester compound in the composite seaweed fertilizer of Example 1 and 1.5kg of water), and the application amount is 600kg / hm 2 The application method is to spray it on the cucumber leaves.

[0052] Treatment 2 is experimental group 2. Treatment method: starting from the time when cucumbers are planted and seedlings have been established, the seaweed fertilizer dilution in the composite seaweed fertilizer of Example 2 is applied once every 15 days (the seaweed fertilizer dilution is prepared by mixing 1 g of the composite seaweed fertilizer of Example 2 with 1.5 kg of water), for a total of 3 applications, with each application amount being 600 kg / hm2. 2 The application method is to spray on the cucumber leaves; and on the 20th day after the cucumbers are planted and the seedlings are slowly grown, the pyridine ester compound dilution in the composite seaweed fertilizer of Example 2 is applied once (the pyridine ester compound dilution is prepared by uniformly stirring 0.015g of the pyridine ester compound in the composite seaweed fertilizer of Example 2 and 1.5kg of water), and the application amount is 600kg / hm 2 The application method is to spray it on the cucumber leaves.

[0053] Treatment 3 is experimental group 3. Treatment method: starting from the time when cucumbers are planted and seedlings have been established, the seaweed fertilizer dilution in the composite seaweed fertilizer of Example 3 is applied once every 15 days (the seaweed fertilizer dilution is prepared by mixing 1 g of the composite seaweed fertilizer of Example 3 with 1.5 kg of water), for a total of 3 applications, with each application amount being 600 kg / hm2. 2 The application method is to spray on the cucumber leaves; and on the 20th day after the cucumbers are planted and the seedlings are slowly grown, the pyridine ester compound dilution in the composite seaweed fertilizer of Example 3 is applied once (the pyridine ester compound dilution is prepared by uniformly stirring 0.015g of the pyridine ester compound in the composite seaweed fertilizer of Example 3 and 1.5kg of water), and the application amount is 600kg / hm 2 The application method is to spray it on the cucumber leaves.

[0054] Treatment 4 is experimental group 4. Treatment method: starting from the time when cucumbers are planted and seedlings have been established, the seaweed fertilizer dilution in the composite seaweed fertilizer of Example 4 is applied once every 15 days (the seaweed fertilizer dilution is prepared by mixing 1 g of the composite seaweed fertilizer of Example 4 with 1.5 kg of water), for a total of 3 applications, with each application amount being 600 kg / hm2. 2The application method is to spray it on the cucumber leaves; and on the 20th day after the cucumbers are planted and the seedlings are slowly grown, the pyridine ester compound dilution in the composite seaweed fertilizer of Example 4 is applied once (the pyridine ester compound dilution is prepared by uniformly stirring 0.015g of the pyridine ester compound in the composite seaweed fertilizer of Example 4 and 1.5kg of water), and the application amount is 600kg / hm 2 The application method is to spray it on the cucumber leaves.

[0055] Treatment 5 is experimental group 5. Treatment method: starting from the time when cucumbers are planted and seedlings have been established, the seaweed fertilizer dilution in the composite seaweed fertilizer of Example 5 is applied once every 15 days (the seaweed fertilizer dilution is prepared by mixing 1 g of the composite seaweed fertilizer of Example 5 with 1.5 kg of water), for a total of 3 applications, with each application amount being 600 kg / hm2. 2 The application method is to spray on the cucumber leaves; and on the 20th day after the cucumbers are planted and the seedlings are hardened, the pyridine ester compound dilution in the composite seaweed fertilizer of Example 5 is applied once (the pyridine ester compound dilution is prepared by mixing 0.015g of the pyridine ester compound in the composite seaweed fertilizer of Example 5 with 1.5kg of water), and the application amount is 600kg / hm 2 The application method is to spray it on the cucumber leaves.

[0056] Treatment 6 is control group 1. Treatment method: starting from the time when cucumbers are planted and seedlings have recovered, the diluted seaweed fertilizer in the composite seaweed fertilizer of comparative example 1 is applied once every 15 days (the diluted seaweed fertilizer is prepared by mixing 1g of the composite seaweed fertilizer in comparative example 1 with 1.5kg of water), for a total of 3 applications, with each application amount of 600kg / hm2. 2 The application method is to spray it on the cucumber leaves; and on the 20th day after the cucumbers are planted and the seedlings are hardened, the diethylaminoethyl ester dilution solution in the composite seaweed fertilizer of comparative example 1 is applied once (the diethylaminoethyl ester dilution solution is prepared by uniformly mixing 0.015g of diethylaminoethyl ester with 1.5kg of water), and the application amount is 600kg / hm 2 The application method is to spray it on the cucumber leaves.

[0057] Treatment 7 is control group 2. Treatment method: starting from the time when cucumbers are planted and seedlings have recovered, the diluted seaweed fertilizer in the composite seaweed fertilizer of comparative example 2 is applied once every 15 days (the diluted seaweed fertilizer is prepared by mixing 1g of the composite seaweed fertilizer in comparative example 2 with 1.5kg of water), for a total of 3 applications, with each application amount of 600kg / hm2. 2The application method is to spray it on the cucumber leaves; and on the 20th day after the cucumbers are planted and the seedlings are hardened, the pyridine ester compound dilution in the composite seaweed fertilizer of Comparative Example 2 is applied once (the pyridine ester compound dilution is prepared by mixing 0.015g of the pyridine ester compound in the composite seaweed fertilizer of Comparative Example 2 with 1.5kg of water), and the application amount is 600kg / hm 2 The application method is to spray it on the cucumber leaves.

[0058] Treatment 8 is control group 3. Treatment method: starting from the time when cucumbers are planted and seedlings have recovered, the diluted seaweed fertilizer in the composite seaweed fertilizer of comparative example 3 is applied once every 15 days (the diluted seaweed fertilizer is prepared by mixing 1g of the composite seaweed fertilizer in comparative example 3 with 1.5kg of water). It is applied 3 times in total, and the application rate for each application is 600kg / hm2. 2 The application method is to spray it on the cucumber leaves; and on the 20th day after the cucumbers are planted and the seedlings are slowly grown, the pyridine ester compound dilution in the composite seaweed fertilizer of Comparative Example 3 is applied once (the pyridine ester compound dilution is prepared by mixing 0.015g of the pyridine ester compound in the composite seaweed fertilizer of Comparative Example 3 with 1.5kg of water), and the application amount is 600kg / hm 2 The application method is to spray it on the cucumber leaves.

[0059] Treatment 9 is control group 4. Treatment method: starting from the time when cucumbers are planted and seedlings have been established, the diluted seaweed fertilizer in the composite seaweed fertilizer of comparative example 4 is applied once every 15 days (the diluted seaweed fertilizer is prepared by mixing 1 g of the composite seaweed fertilizer of comparative example 4 with 1.5 kg of water), for a total of 3 applications, with each application amount of 600 kg / hm2. 2 The application method is to spray it on the cucumber leaves; and on the 20th day after the cucumbers are planted and the seedlings are slowly grown, the pyridine ester compound dilution in the composite seaweed fertilizer of Comparative Example 4 is applied once (the pyridine ester compound dilution is prepared by mixing 0.015g of the pyridine ester compound in the composite seaweed fertilizer of Comparative Example 4 with 1.5kg of water), and the application amount is 600kg / hm 2 The application method is to spray it on the cucumber leaves.

[0060] Treatment 10 is control group 5. Treatment method: starting from the time the cucumbers are planted and the seedlings have grown, the diluted seaweed fertilizer in the composite seaweed fertilizer of comparative example 5 is applied once every 15 days (the diluted seaweed fertilizer is prepared by mixing 1 g of the composite seaweed fertilizer in comparative example 5 with 1.5 kg of water), for a total of 3 applications, with each application amount being 600 kg / hm2. 2The application method is to spray it on the cucumber leaves; and on the 20th day after the cucumbers are planted and the seedlings are slowly grown, the pyridine ester compound dilution in the composite seaweed fertilizer of Comparative Example 5 is applied once (the pyridine ester compound dilution is prepared by mixing 0.015g of the pyridine ester compound in the composite seaweed fertilizer of Comparative Example 5 with 1.5kg of water), and the application amount is 600kg / hm 2 The application method is to spray it on the cucumber leaves.

[0061] Treatment 11 was the seaweed fertilizer control group. The treatment method was as follows: starting from the time the cucumbers were planted and the seedlings were grown, the seaweed fertilizer dilution in the composite seaweed fertilizer of Example 1 was applied once every 15 days (the seaweed fertilizer dilution was prepared by mixing 1 g of the composite seaweed fertilizer of Example 1 with 1.5 kg of water). The application was carried out 3 times in total, with an application rate of 600 kg / hm2 each time. 2 The application method is to spray it on the cucumber leaves.

[0062] Treatment 12 was a pyridinium ester compound control group. Treatment method: 20 days after the cucumbers were planted and seedlings were hardened, the pyridinium ester compound dilution in the composite seaweed fertilizer of Example 1 was applied once (the pyridinium ester compound dilution was prepared by mixing 0.015 g of the pyridinium ester compound in the composite seaweed fertilizer of Example 1 with 1.5 kg of water), with an application rate of 600 kg / hm2. 2 The application method is to spray it on the cucumber leaves.

[0063] Treatment 13 was the water control group. Treatment method: starting from the time the cucumbers were planted and seedlings were hardened, water was applied once every 15 days for a total of 3 times, with each application rate of 600 kg / hm2. 2 The application method is to spray on the cucumber leaves; and apply clean water once on the 20th day after the cucumber seedlings are transplanted, with an application rate of 600kg / hm 2 The application method is to spray it on the cucumber leaves.

[0064] During the experiment, the cucumber yield, cucumber length, transverse diameter, flesh thickness, protein, total sugar, fat, vitamin B2, vitamin C, iron and calcium contents of different treatments were statistically analyzed; the cucumber yield was based on the yield from the beginning of harvest to 60 days; the protein content was determined according to the Kjeldahl method in standard GB5009.5-85, the fat content was determined according to the Soxhlet extraction method in standard GB5009.6-85, the total sugar content was determined according to the potassium permanganate titration method in standard GB5009.7-85, the vitamin B2 content was determined by fluorescence spectrometry, the vitamin C content was determined by 2,6-dichlorophenol indophenol method, and the iron and calcium contents were determined by inductively coupled plasma optical emission spectrometry.

[0065] When measuring cucumber yield, the average yield of the three plots corresponding to each treatment was used as the final experimental result. When measuring cucumber length, diameter, flesh thickness, and the content of protein, total sugar, fat, vitamin B2, vitamin C, iron, and calcium in cucumbers, 10 cucumbers were randomly selected from the harvested cucumbers of each treatment and the average value of the measurements was used as the final experimental result. The experimental results are shown in Tables 1 and 2.

[0066] Table 1 Effects of composite seaweed fertilizers in various embodiments and comparative examples on cucumber yield and fruit appearance

[0067]

[0068]

[0069] Note: Different lowercase letters in the same column indicate significant differences at the 5% level.

[0070] Table 2 Effects of composite seaweed fertilizers in various embodiments and comparative examples on the nutritional components of cucumber fruits

[0071]

[0072] Note: Different lowercase letters in the same column indicate significant differences at the 5% level.

[0073] It can be seen from the test results in Table 1 and Table 2 that, compared with using seaweed fertilizer diluent or pyridinium ester compound diluent alone, the composite seaweed fertilizer of the present invention can significantly increase the yield, cucumber length, transverse diameter, flesh thickness, and protein, total sugar, fat, vitamin B2, vitamin C, iron and calcium content of cucumbers, proving that the seaweed fertilizer and the pyridinium ester compound synthesized in the present invention have a synergistic effect, which has a significant effect on improving the growth of cucumbers and the quality of fruits.

[0074] In addition, from the comparison results of treatment 1, treatment 4 and treatment 5, it can be seen that when the acyl chloride compound used in the preparation of pyridine ester compounds is replaced with 4-chlorobutyryl chloride or chloroacetyl chloride, the change in the carbon chain length on one side of the ester bond may cause the lipophilicity and hydrophilicity of the pyridine ester compound to change, affecting the effect of the pyridine ester compound and resulting in a decrease in cucumber yield and quality.

[0075] Finally, as shown in treatment 1 and treatment 6-8, when pyridinium ester compounds are replaced by conventional aminoethyl hexanoate or when the acyl chloride compound when preparing pyridinium ester compounds is replaced by propionyl chloride or butyryl chloride, due to the lack of chlorine element, the pyridinium ester compound action intensity is weakened, and then the yield and quality of cucumbers are significantly reduced. As shown in treatment 1 and treatment 9-10, the seaweed organic fertilizer prepared by using Ascophyllum nodosum, kelp and grapefruit peel can improve the yield and quality of cucumbers compared to the organic fertilizer prepared by using Ascophyllum nodosum, kelp or grapefruit peel alone. As shown in treatment 1 and treatment 11-12, when using seaweed fertilizer or pyridinium ester compounds to process cucumbers alone, the yield and quality of cucumbers are reduced, which once again proves that the seaweed fertilizer in the present invention and the synthetic pyridinium ester compounds have a synergistic effect, and the growth of cucumbers and the improvement of fruit quality have a significant lifting effect.

Claims

1. A composite seaweed fertilizer, characterized in that: The invention comprises a pyridine ester compound and seaweed fertilizer, wherein the mass of the pyridine ester compound is 1.5-2.5% of the mass of the seaweed fertilizer; the structure of the pyridine ester compound is as follows: Wherein, R is a C1-C3 alkylene group; The preparation method of the seaweed fertilizer is as follows: mixing Ascophyllum nodosum, kelp, pomelo peel and water and then crushing them to obtain a crushed liquid; then enzymatically hydrolyzing the crushed liquid to obtain the seaweed fertilizer; the mass ratio of Ascophyllum nodosum, kelp and pomelo peel is 3-4:3-4:1-1.

5.

2. The composite seaweed fertilizer according to claim 1, wherein The R is an ethylene group.

3. The composite seaweed fertilizer according to claim 1 or 2, wherein The pyridine ester compound is prepared by reacting 2-propanol pyridine and an acyl chloride compound, wherein the acyl chloride compound is 3-chloropropionyl chloride, 4-chlorobutyryl chloride or chloroacetyl chloride, and the molar ratio of the 2-propanol pyridine to the acyl chloride compound is 1:1.1-1.

2.

4. The composite seaweed fertilizer according to claim 1 or 2, wherein The mass ratio of the ascophyllum nodosum, kelp, pomelo peel and water is 3-4:3-4:1-1.5:10; the complex enzyme used in the enzymatic hydrolysis is composed of protease, pectinase, oxidoreductase and cellulase in a mass ratio of 20:8:15:35; the mass ratio of the crushing liquid and the complex enzyme is 100:1, the pH value during the enzymatic hydrolysis is 6.5-7.5, the temperature is 35-40°C, and the time is 70-80 hours.

5. An application of the composite seaweed fertilizer according to any one of claims 1 to 4.

6. The use according to claim 5, characterized in that The application comprises the following steps: spraying the pyridine ester compound and the seaweed fertilizer in the composite seaweed fertilizer on the leaves of the plants in the form of diluted solutions respectively.

7. The use according to claim 6, characterized in that The plant is cucumber; the spraying is carried out after the cucumber is planted and the seedlings have grown, and the seaweed fertilizer diluted solution is sprayed once every 15 to 17 g, and the spraying amount each time is 600 to 700 kg / hm 2 18-20 days after cucumbers are transplanted and seedlings are hardened, spray the diluted solution of pyridine ester compounds once, with a spraying rate of 600-700 kg / hm2. 2 The seaweed fertilizer dilution liquid is prepared by mixing the seaweed fertilizer and water in a mass ratio of 1-1.5 g:1.5 kg, and the pyridine ester compound dilution liquid is prepared by mixing the pyridine ester compound and water in a mass ratio of 0.0015-0.0025 g:1.5 kg.

8. The use according to claim 7, characterized in that The application is to increase the yield of cucumber.

9. The use according to claim 7, characterized in that The application is to increase the length, transverse diameter and flesh thickness of cucumbers.

10. The use according to claim 7, characterized in that The application is to increase the content of protein, total sugar, fat, vitamin B2, vitamin C, iron and calcium in cucumbers. The molar ratio of the ketodiamide dinitrile compound to hydroxylamine hydrochloride is 1:4.2-4.5.

Images