Special bacterial fertilizer for sorghum and application of special bacterial fertilizer in prevention and control of sorghum diseases

Abstract

The invention discloses a special bacterial fertilizer for sorghum and application of the special bacterial fertilizer in prevention and control of sorghum diseases, and belongs to the technical field of microbial fertilizers and agriculture. The special bacterial fertilizer for sorghum comprises the following components in parts by weight: 8-10 parts of a composite microbial agent, 40-50 parts of sorghum residues, 5-10 parts of bentonite, 1-2 parts of potassium humate, 1-2 parts of composite trace elements, 10-20 parts of sawdust and 10-20 parts of rice bran, wherein the complex microbial inoculant consists of a bacillus subtilis microbial inoculant, a rhizobium microbial inoculant, a bacillus velezensis microbial inoculant and a trichoderma harzianum microbial inoculant. The special bacterial fertilizer for sorghum provided by the invention contains rich microorganisms and nutrient substances, and can effectively prevent and control sorghum diseases and enhance the disease resistance of sorghum. The components such as microorganisms and organic acids in the special bacterial fertilizer for sorghum can promote the formation of soil organic matters, improve the soil structure, improve the soil quality and effectively improve the growth environment of sorghum.

Description

Technical Field

[0001] The invention belongs to the field of microbial fertilizers and agricultural technology, and particularly relates to a special microbial fertilizer for sorghum and application thereof in preventing and controlling sorghum diseases. Background Art

[0002] Sorghum is a crucial crop in dryland agriculture worldwide. It can be used for food, feed, and brewing, and is also a key raw material for industrial and energy development, boasting high comprehensive utilization value. In the context of global climate change, sorghum, a key crop with strong resistance to multiple stresses, including drought, flooding, high temperatures, salinity, and infertility, plays a crucial role in the development of the crop industry.

[0003] Leaf diseases are a significant factor affecting sorghum growth at all stages of its growth cycle. These include major diseases such as sorghum anthracnose, sorghum purple spot, and sorghum target spot, which severely impact sorghum plant health and yield. Currently, the primary method for controlling sorghum diseases is chemical pesticides, such as thiophanate-methyl, carbendazim, triadimefon, difenoconazole, tebuconazole, and iprodione. However, the long-term use of single chemical control methods carries a range of risks, including increased resistance to pesticides, harm to human health, damage to beneficial organisms, and disruption of ecological balance. Therefore, to improve sorghum yield and quality and ensure safe and efficient sorghum production, it is imperative to develop an environmentally friendly, green, and efficient method for controlling sorghum diseases. Summary of the Invention

[0004] To address the challenges of sorghum production, the present invention aims to provide a sorghum-specific bacterial fertilizer and its use in preventing and controlling sorghum diseases. By applying a composite bacterial agent that antagonizes sorghum disease pathogens, promotes sorghum growth, and enhances sorghum's stress resistance, combined with organic matter, bentonite, potassium humate, and complex trace elements, the sorghum-specific bacterial fertilizer effectively prevents and controls sorghum diseases and enhances sorghum's disease resistance.

[0005] To achieve the above object, the present invention provides the following technical solutions:

[0006] One of the technical solutions of the present invention is to provide a special bacterial fertilizer for sorghum, which comprises, by weight, 8 to 10 parts of a composite bacterial agent, 40 to 50 parts of sorghum residue, 5 to 10 parts of bentonite, 1 to 2 parts of potassium humate, 1 to 2 parts of composite trace elements, 10 to 20 parts of sawdust and 10 to 20 parts of rice bran; the composite bacterial agent is composed of a Bacillus subtilis agent, a Rhizobium agent, a Bacillus Velezius agent and a Trichoderma harzianum agent.

[0007] The composite bacterial agent in the special bacterial fertilizer for sorghum of the present invention is composed of Bacillus subtilis agent, Rhizobium agent, Bacillus velezii agent and Trichoderma harzianum agent. The composite bacterial agent of this combination can not only antagonize a variety of sorghum disease pathogens and improve the disease resistance of sorghum, but also improve the soil, increase fertility, improve the growth performance and stress resistance of sorghum, and at the same time enable it to have a certain insect resistance. The sorghum residue in the special bacterial fertilizer for sorghum is an organic fertilizer, which contains a large amount of organic matter, can provide sufficient nutrients for sorghum and improve the fertility of the soil; bentonite has physical and chemical properties such as adsorption, expansion and contraction, and cation exchange, which can adsorb and retain nutrients in the bacterial fertilizer and has a slow-release effect. On the other hand, the beneficial organisms and Na contained in the bentonite + , K + , Ca 2+ Major and trace elements such as humic acid can also promote the growth and development of sorghum roots; potassium humate, as a high-efficiency organic potassium fertilizer, can increase the content of available potassium in the soil, and at the same time has strong adsorption, exchange, complexation and chelation capabilities, enhancing the stress resistance of sorghum and effectively improving soil properties; complex trace elements can promote the growth and reproduction of complex bacterial agents and beneficial microorganisms, and rebuild a good ecological environment for soil microorganisms; sawdust and rice bran can not only serve as carbon sources for microorganisms, but their good air permeability and water retention can serve as carriers for microbial growth and reproduction, which is beneficial to microbial growth.

[0008] Preferably, the mass ratio of the Bacillus subtilis agent, the Rhizobium agent, the Bacillus Velezii agent and the Trichoderma harzianum agent is 1-2:1-2:1-2:1-2.

[0009] More preferably, the effective viable bacteria count of the Bacillus subtilis agent is 20 to 40 billion / g; the effective viable bacteria count of the Rhizobium agent is 200 to 320 million / g; the effective viable bacteria count of the Bacillus Velezii agent is 1 to 2 billion / g; and the effective viable bacteria count of the Trichoderma harzianum agent is 200 to 300 million / g.

[0010] Preferably, the trace elements in the composite trace elements include iron, manganese, molybdenum, copper, boron and zinc.

[0011] More preferably, the composite trace elements are a mixture of ferrous sulfate, manganese sulfate, ammonium molybdate, copper sulfate, borax and zinc sulfate in equal mass ratios.

[0012] The second technical solution of the present invention is to provide an application of the above-mentioned special bacterial fertilizer for sorghum in the preparation of bacterial fertilizer for preventing and resisting sorghum anthracnose.

[0013] The third technical solution of the present invention is to provide an application of the above-mentioned special bacterial fertilizer for sorghum in the preparation of bacterial fertilizer for preventing and resisting sorghum purple spot disease.

[0014] The fourth technical solution of the present invention is to provide an application of the above-mentioned special bacterial fertilizer for sorghum in the preparation of bacterial fertilizer for preventing and resisting sorghum target spot disease.

[0015] The fifth technical solution of the present invention is to provide an application of the above-mentioned sorghum-specific bacterial fertilizer in sorghum cultivation.

[0016] Preferably, the application amount of the sorghum-specific bacterial fertilizer is 50 to 100 kg / mu.

[0017] The beneficial technical effects of the present invention are as follows:

[0018] The special bacterial fertilizer for sorghum provided by the invention contains rich microorganisms and nutrients, and can enhance the disease resistance of sorghum.

[0019] By applying a specialized bacterial fertilizer specifically for sorghum, this method can reduce the use of chemical fertilizers and pesticides, lowering production costs while also mitigating pollution and protecting the soil ecosystem. Compared to conventional fertilization methods, using bacterial fertilizer to grow sorghum significantly reduces soil and water pollution from harmful substances such as chemical pesticides.

[0020] The microorganisms, organic acids and other components in the special bacterial fertilizer for sorghum provided by the present invention can promote the formation of organic matter, improve soil structure, enhance soil quality, and effectively improve the growth environment of sorghum. DETAILED DESCRIPTION

[0021] Sorghum anthracnose, caused by the fungus C. sublineola, can damage sorghum leaves, stalks, stalks, ears, and grains, resulting in a yield reduction of over 69%, seriously threatening sorghum yield and quality. The pathogen overwinters in weeds and diseased plant debris in the soil. It can directly infect seedling roots or stem bases through soil debris, or it can overwinter as mycelium or conidia on seeds, becoming the primary source of infection the following year. Anthracnose can occur at all growth stages of sorghum, but develops most rapidly during the booting stage. The rising temperatures, long sunshine hours, and high humidity of June and July each year favor the occurrence and spread of the disease. Sorghum anthracnose lesions often begin at the leaf tips and are round or oval in shape, with a reddish-brown center and purple-red, orange-red, or brown edges. Lesions on leaf sheaths are oval or elongated, red, purple, or black. Later, small black conidiophores form on the lesions, and in severe cases, the leaves partially die.

[0022] The present invention uses a composite bacterial agent composed of Bacillus subtilis, Rhizobium, Bacillus Velezii and Trichoderma harzianum to antagonize sorghum anthracnose pathogens by competing for nutrients with them, secreting antibacterial substances and triggering sorghum's self-defense response, thereby effectively reducing the occurrence of the soil-borne disease sorghum anthracnose and even preventing its further spread.

[0023] The causative agent of sorghum purple leaf spot is Cercospora sorghum, which primarily attacks leaves, sheaths, and stems, typically in the late stages of growth. The pathogen overwinters as mycelium and conidia on diseased field debris, other weeds, and seeds, serving as a primary source of infection the following year. High temperatures, high humidity, and heavy rain favor the growth, reproduction, and spread of purple leaf spot. Sorghum purple leaf spot primarily infects the lower leaves. Lesions expand from small red spots to elliptical to oblong shapes, often confined to the space between parallel veins. Lesions are purple-red with no distinct edges or sometimes with a pale purple halo. When humidity is high, dense gray mold grows on both sides of the leaf lesions, producing large numbers of conidia.

[0024] The composite bacterial agent provided by the present invention fully utilizes the favorable conditions of the accumulation of dominant bacterial communities and nutrients, competes with pathogenic bacteria for nutritional space, and lays the foundation for sorghum to quickly absorb nutrients, thereby effectively improving the growth ability and disease resistance of sorghum and significantly inhibiting the infection and expansion of sorghum Cercospora kaoliang.

[0025] The causative agent of sorghum target spot disease is Helminthosporium bilateri, which can cause yield losses of over 50% in susceptible varieties. The pathogen overwinters in diseased plant debris in the soil. The following year, the mycelium produces conidiophores and conidia, which serve as the primary source of infection. Multiple reinfections occur during a single growing season, gradually exacerbating the disease. Target spot disease can occur at all stages of sorghum growth, with the disease spreading most rapidly during the hot and rainy months of July and August. Target spot disease initially appears as small, pale purple-red spots on leaves, which expand into oval, elliptical, or oblong lesions with a distinct concentric ring of brown or purple-red necrosis at their centers. In severe cases, leaves gradually become diseased and die from the bottom up, resulting in yield losses.

[0026] The composite bacterial agent provided by the present invention improves the competitiveness of biocontrol bacteria with pathogens. It prevents the invasion of target spot disease pathogens and reduces the risk of sorghum target spot disease through multiple mechanisms such as secreting antibacterial active metabolites, competing with sorghum helices for nutrition and space, and inducing sorghum plant immunity.

[0027] Furthermore, the combination of microorganisms in the compound inoculant makes it difficult for pathogens to develop tolerance to the antimicrobial mechanisms of multiple microorganisms, hindering the development of resistance and thus prolonging the effectiveness of the microbial inoculant. While combating pathogens, the compound inoculant also plays a positive role by restoring soil microbial communities, improving soil structure, and promoting sorghum plant growth. Trichoderma can secrete plant growth hormones, which promote sorghum growth; while Bacillus, while secreting plant growth hormones, can increase soil porosity, improve soil structure, and enhance soil fertility. This combined effect helps sorghum plants better adapt to environmental stresses and enhance their disease resistance.

[0028] On the other hand, sorghum is a plant that can coexist with rhizobia. This symbiotic relationship enables sorghum to fix nitrogen from the air into the soil, thereby improving soil fertility. After the rhizobia invade the sorghum roots, they secrete substances that stimulate the proliferation of root hair cells to form nodules. The rhizobia fix nitrogen in the nodules and make it available to the sorghum. This nitrogen fixation is important for sorghum growth and soil fertility. The symbiotic relationship between sorghum and rhizobia increases the nitrogen content of the soil, improves the physical and chemical properties of the soil, and provides a favorable environment for the growth and reproduction of soil microorganisms.

[0029] The special bacterial fertilizer for sorghum provided by the invention can improve soil fertility and enhance the growth performance and stress resistance of sorghum by antagonizing pathogenic bacteria of sorghum diseases.

[0030] At present, the development of sorghum bacterial fertilizers is mainly focused on the field of increasing sorghum yield, such as EM bacterial fertilizers, and the development of disease-resistant sorghum fertilizers is still in a blank stage.

[0031] Various exemplary embodiments of the present invention are now described in detail. This detailed description should not be considered as a limitation of the present invention, but should be understood as a more detailed description of certain aspects, features, and embodiments of the present invention. It should be understood that the terms used in the present invention are only for describing specific embodiments and are not intended to limit the present invention.

[0032] In addition, for numerical ranges in the present invention, it is understood that each intervening value between the upper and lower limits of the range is also specifically disclosed. Each smaller range between any stated value or stated range, and any other stated value or intervening value in the stated range, is also included in the present invention. The upper and lower limits of these smaller ranges may independently be included or excluded in the range.

[0033] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which the invention pertains. Although preferred methods and materials have been described herein, any methods and materials similar or equivalent to those described herein can also be used in the practice or testing of the present invention.

[0034] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.

[0035] The Bacillus subtilis inoculant used in the examples and comparative examples of the present invention is a commercially available variety, and the effective viable count is 20 billion / g.

[0036] The rhizobium agent used in the examples and comparative examples of the present invention is a soybean rhizobium agent, a commercially available variety, with an effective viable bacterial count of 320 million / g.

[0037] The Bacillus velezensis agent used in the examples and comparative examples of the present invention is a commercially available variety, and the effective viable count is 1 billion / g.

[0038] The Trichoderma harzianum fungal agent used in the examples and comparative examples of the present invention is a commercially available variety, and the effective viable bacteria count is 200 million / g.

[0039] The composite trace elements used in the examples and comparative examples of the present invention are a mixture of ferrous sulfate, manganese sulfate, ammonium molybdate, copper sulfate, borax and zinc sulfate in equal mass ratios.

[0040] Example 1

[0041] The raw materials were prepared according to 10 parts by weight of a composite microbial agent (Bacillus subtilis agent: soybean rhizobium agent: Bacillus velezii agent: Trichoderma harzianum agent = 1:1:1:1, w / w), 45 parts by weight of sorghum residue, 8 parts by weight of bentonite, 2 parts by weight of potassium humate, 1.5 parts by weight of complex trace elements, 15 parts by weight of sawdust and 15 parts by weight of rice bran, and the mixture was uniformly mixed to obtain a microbial fertilizer.

[0042] Example 2

[0043] The raw materials were prepared according to 10 parts by weight of a composite microbial agent (Bacillus subtilis agent: soybean rhizobium agent: Bacillus velezii agent: Trichoderma harzianum agent = 2:1:2:2, w / w), 50 parts by weight of sorghum residue, 10 parts by weight of bentonite, 1 part by weight of potassium humate, 1 part by weight of complex trace elements, 10 parts by weight of sawdust and 20 parts by weight of rice bran, and the mixture was uniformly mixed to obtain a microbial fertilizer.

[0044] Example 3

[0045] The raw materials were prepared according to 10 parts by weight of a composite microbial agent (Bacillus subtilis agent: soybean rhizobium agent: Bacillus velezii agent: Trichoderma harzianum agent = 1:2:1:1, w / w), 40 parts by weight of sorghum residue, 5 parts by weight of bentonite, 2 parts by weight of potassium humate, 2 parts by weight of complex trace elements, 20 parts by weight of sawdust and 10 parts by weight of rice bran, and the mixture was uniformly mixed to obtain a microbial fertilizer.

[0046] Comparative Example 1

[0047] The raw materials were prepared according to 10 parts by weight of a composite microbial agent (Bacillus subtilis agent: soybean rhizobium agent: Trichoderma harzianum agent = 2:1:1, w / w), 45 parts by weight of sorghum residue, 8 parts by weight of bentonite, 2 parts by weight of potassium humate, 1.5 parts by weight of complex trace elements, 15 parts by weight of sawdust and 15 parts by weight of rice bran, and the mixture was uniformly mixed to obtain a microbial fertilizer.

[0048] Comparative Example 2

[0049] The raw materials were prepared according to 10 parts by weight of a composite microbial agent (Bacillus subtilis agent: soybean rhizobium agent: Bacillus velezii agent = 2:1:1, w / w), 45 parts by weight of sorghum residue, 8 parts by weight of bentonite, 2 parts by weight of potassium humate, 1.5 parts by weight of complex trace elements, 15 parts by weight of sawdust and 15 parts by weight of rice bran, and the mixture was uniformly mixed to obtain a microbial fertilizer.

[0050] Test Example 1

[0051] Investigate the control ability of the bacterial fertilizers prepared in Examples 1 to 3 and Comparative Examples 1 to 2 on sorghum anthracnose

[0052] Methods: Six planting plots were selected. Five of the plots were treated with 100 kg / mu of each microbial fertilizer as basal fertilizer. One plot remained unfertilized and served as a blank control. Each plot had 10 planting rows, 5 m long, 55 cm apart, and a density of 8,080 plants / mu. The high-quality, high-yielding hybrid glutinous sorghum variety "Jinyu Nuo 3," suitable for local cultivation, was selected. A spore suspension of Colletotrichum sorghum was inoculated onto sterilized seeds and incubated for 7 days to allow full infection. Using the infected seed inoculation method, when the sorghum had 4-5 leaves and 1 heart, infected seeds were inoculated into the leaf sheaths of the 3rd to 4th leaf, with 3-5 seeds per plant. The day after inoculation, irrigation and spraying were carried out according to weather conditions to maintain the required humidity for disease development. Conventional field management was followed. Disease occurrence was investigated during sorghum maturity, and the disease index and control effectiveness were calculated.

[0053] The disease severity grading standards for sorghum anthracnose are shown in Table 1, and the control effects of each treatment group are shown in Table 2.

[0054] Table 1 Sorghum anthracnose disease severity grading standards

[0055] Disease level Lesion area and description 0 No obvious symptoms 1 The lesion area accounts for 1-10% of the leaf area 2 The lesion area accounts for 11-25% of the leaf area, and no conidiophores are produced. 3 The lesion area accounts for 26-50% of the leaf area and produces conidia. 4 The lesion area accounts for more than 50% of the leaf area, and conidia are produced.

[0056] Note: Disease index = (∑(number of diseased leaves at each level × representative value of each level)) / (total number of leaves surveyed × highest representative value) × 100;

[0057] Control effect = (disease index of blank control area - disease index of treated area) / disease index of blank control area × 100%. (The same below)

[0058] Table 2 Control effect of each treatment group on sorghum anthracnose

[0059]

[0060]

[0061] Table 2 shows that the sorghum-specific bacterial fertilizer prepared in Examples 1-3 of the present invention has the ability to prevent and resist sorghum anthracnose. The synergistic disease resistance of Bacillus subtilis, soybean rhizobium, Bacillus velezii and Trichoderma harzianum is good, and the prevention efficiency can reach more than 62%, which is better than the 26% to 41% prevention efficiency of the composite bacterial agent in which Bacillus velezii or Trichoderma harzianum is replaced with the same mass of Bacillus subtilis.

[0062] Test Example 2

[0063] Investigate the control ability of the bacterial fertilizers prepared in Examples 1 to 3 and Comparative Examples 1 to 2 on sorghum purple spot disease

[0064] Methods: Six planting plots were selected. Five of the plots were treated with 100 kg / mu of each microbial fertilizer as basal fertilizer. One plot remained unfertilized and served as a blank control. Each plot had 10 planting rows, 5 m long, 55 cm apart, and a density of 8,080 plants / mu. A high-quality, high-yielding hybrid glutinous sorghum variety, "Jinyu Nuo No. 3," suitable for local cultivation, was selected. Sterilized sorghum seeds were inoculated with Cercospora sorghumium for 5-7 days. The infected seeds were then inoculated in the field. When the sorghum had 4-5 leaves and 1 heart, 3-5 seeds were inoculated per plant into the leaf sheaths of the third to fourth leaves. The day after inoculation, irrigation and spraying were carried out according to weather conditions to maintain the required humidity for disease. Conventional field management was followed. Disease occurrence was investigated at sorghum maturity, and the disease index and control effectiveness were calculated.

[0065] The disease severity grading standards for sorghum purple spot are shown in Table 3. The statistical results of sorghum purple spot prevention and control effects in each group are shown in Table 4.

[0066] Table 3 Disease severity grading standards for sorghum purple spot

[0067]

[0068]

[0069] Table 4 The effect of each treatment group on the prevention and resistance of sorghum purple spot

[0070] Group Disease index Prevention effect% Example 1 5.50 74.41 Example 2 4.00 81.40 Example 3 6.00 72.10 Comparative Example 1 14.00 34.88 Comparative Example 2 17.50 18.60 Blank group 21.50 _

[0071] As can be seen from Table 4, the sorghum-specific bacterial fertilizers prepared in Examples 1-3 of the present invention have the ability to prevent and resist sorghum purple spot disease. The synergistic disease resistance of Bacillus subtilis, soybean rhizobium, Bacillus velezii and Trichoderma harzianum is good, and the prevention efficiency can reach more than 72%, which is better than the 18-34% prevention efficiency of the composite bacterial agent in which Bacillus velezii or Trichoderma harzianum is replaced by the same mass of Bacillus subtilis.

[0072] Test Example 3

[0073] Investigate the control ability of the bacterial fertilizers prepared in Examples 1 to 3 and Comparative Examples 1 to 2 on sorghum target spot disease

[0074] Methods: Six planting plots were selected. Five of the plots were treated with 100 kg / mu of microbial fertilizer from each group. One plot remained unfertilized and served as a blank control. Each plot had 10 planting rows, 5 m long, 55 cm apart, and a density of 8,080 plants / mu. The high-quality, high-yielding hybrid glutinous sorghum variety "Jinyu Nuo No. 3," suitable for local cultivation, was selected. Using the inoculation method, sorghum kernels infected with target spot disease (prepared by inoculating sterilized seeds with a spore suspension of the pathogen) were inoculated into the sorghum plant's bell stage, with 3 to 5 kernels per plant. Conventional field management was followed, and disease activity was assessed at the physiological maturity stage of the sorghum kernels. Disease index and control efficacy were calculated.

[0075] The disease severity grading standards for sorghum target leaf spot are shown in Table 5. The statistical results of the sorghum target leaf spot air defense effects in each group are shown in Table 6.

[0076] Table 5 Sorghum target spot disease severity grading standards

[0077]

[0078]

[0079] Table 6 The effect of each treatment group on the prevention and control of sorghum target spot disease

[0080] Group Disease index Prevention effect% Example 1 5.00 65.52 Example 2 2.00 86.21 Example 3 6.00 58.62 Comparative Example 1 9.00 37.93 Comparative Example 2 10.00 31.03 Blank group 14.50 _

[0081] As can be seen from Table 6, the sorghum-specific bacterial fertilizers prepared in Examples 1-3 of the present invention have the ability to prevent and resist sorghum target spot disease. The synergistic disease resistance of Bacillus subtilis, soybean rhizobium, Bacillus velezii and Trichoderma harzianum is good, and the prevention efficiency can reach more than 58%, which is better than the 31-37% prevention efficiency of the composite bacterial agent in which Bacillus velezii or Trichoderma harzianum is replaced by the same mass of Bacillus subtilis.

[0082] The embodiments described above are merely descriptions of preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Without departing from the spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by persons skilled in the art should fall within the scope of protection defined by the claims of the present invention.

Claims

1. A special bacterial fertilizer for sorghum, characterized in that: The components include, by weight, 8 to 10 parts of a composite bacterial agent, 40 to 50 parts of sorghum residue, 5 to 10 parts of bentonite, 1 to 2 parts of potassium humate, 1 to 2 parts of composite trace elements, 10 to 20 parts of sawdust and 10 to 20 parts of rice bran; the composite bacterial agent consists of a Bacillus subtilis agent, a Rhizobium agent, a Bacillus velezii agent and a Trichoderma harzianum agent.

2. The sorghum-specific bacterial fertilizer according to claim 1, characterized in that The mass ratio of the Bacillus subtilis agent, the Rhizobium agent, the Bacillus Velezii agent and the Trichoderma harzianum agent is 1-2:1-2:1-2:1-2.

3. The sorghum-specific bacterial fertilizer according to claim 2, characterized in that The effective live bacteria count of the Bacillus subtilis agent is 20 to 40 billion / g; the effective live bacteria count of the Rhizobium agent is 200 to 320 million / g; the effective live bacteria count of the Bacillus Velezii agent is 1 to 2 billion / g; and the effective live bacteria count of the Trichoderma harzianum agent is 200 to 300 million / g.

4. The special bacterial fertilizer for sorghum according to claim 1, characterized in that The trace elements in the composite trace elements include iron, manganese, molybdenum, copper, boron and zinc.

5. The special bacterial fertilizer for sorghum according to claim 4, characterized in that The composite trace elements are a mixture of ferrous sulfate, manganese sulfate, ammonium molybdate, copper sulfate, borax and zinc sulfate in equal mass ratios.

6. Use of the sorghum-specific bacterial fertilizer according to any one of claims 1 to 5 in the preparation of a sorghum anthracnose-resistant bacterial fertilizer.

7. Use of the sorghum-specific bacterial fertilizer according to any one of claims 1 to 5 in the preparation of a bacterial fertilizer resistant to sorghum purple spot disease.

8. Use of the sorghum-specific bacterial fertilizer according to any one of claims 1 to 5 in the preparation of a bacterial fertilizer resistant to sorghum target leaf spot disease.

9. Use of the sorghum-specific bacterial fertilizer according to any one of claims 1 to 5 in growing sorghum.

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