Biopharmaceutical integrated raw material fermentation treatment device

Abstract

The invention belongs to the technical field of fermentation equipment, and particularly relates to a biopharmaceutical integrated raw material fermentation treatment device which comprises a tank body and further comprises a rotating shaft, stirring blades, an oxygen pipe and a cooling water tank, the rotating shaft is arranged in the tank body in the axial direction, and the rotating shaft is sequentially provided with a driving element, a thermosensitive part and blades from top to bottom; a plurality of stirring blades are arranged around the rotating shaft in the tank body, cutting edges are installed on the edges, away from the rotating shaft, of the stirring blades, a spiral assembly is vertically arranged between every two adjacent stirring blades, and the thermosensitive part absorbs heat generated by fermentation of the raw materials to cause expansion and is used for pushing the cutting edges to be attached to the wall in the tank body; the linkage spiral assembly rotates close to the wall and reversely pushes the raw materials relative to the blades. According to the invention, heat generated during fermentation is utilized to push wall adhesion, inner wall attachments can be scraped off, multi-layer stirring can be carried out, the space is opened after cooling, and enough space is reserved for disinfection.

Description

Technical Field

[0001] The present invention belongs to the technical field of fermentation equipment, and in particular relates to an integrated biopharmaceutical raw material fermentation processing device. Background Art

[0002] Biopharmaceutical integrated raw material fermentation tanks typically adopt an integrated design, integrating fermentation, stirring, cooling, heating, and control functions for easy operation and maintenance. Their operating principle is primarily based on the fermentation process of microorganisms. By controlling the environmental conditions within the fermentation tank (such as temperature, pH, oxygen supply, etc.), a sterile and controllable optimal growth environment is provided for microorganisms. Under suitable conditions, microorganisms can synthesize the desired biopharmaceuticals in large quantities, which are then extracted through the separation and purification of the fermentation broth.

[0003] Problems with existing technologies:

[0004] During the microbial fermentation process, the fermentation tank is mainly stirred by rotating blades, including:

[0005] Some manufacturers choose to attach one side of the stirring blade to the wall to scrape off the debris on the inner wall of the fermentation tank. However, the area between the stirring blade and the inner wall of the fermentation tank is extremely narrow and difficult to disinfect, so it is not often used.

[0006] Some manufacturers also choose to use driving elements such as cylinders to move the stirring blades, so that they can stick to the wall or move away from the open distance while stirring. However, the hidden areas such as holes, grooves, and threads in the cylinder itself become areas where microorganisms remain, making it difficult to disinfect them. There is great pressure to balance the scraping and cleaning of the inner wall and internal disinfection, which still needs to be improved. Summary of the Invention

[0007] The purpose of the present invention is to provide an integrated biopharmaceutical raw material fermentation processing device, which can use the heat generated during fermentation to promote wall adhesion, scrape off inner wall attachments and multi-level stirring, open the spacing after cooling, and leave enough space for disinfection, which can well coordinate the inner wall scraping and cleaning and internal disinfection work.

[0008] The technical solutions adopted by the present invention are as follows:

[0009] A biopharmaceutical integrated raw material fermentation processing device, comprising a tank body and:

[0010] A rotating shaft, the rotating shaft being axially arranged inside the tank body, the rotating shaft being provided with a driving element, a heat-sensitive element and blades in order from top to bottom, and the blades being driven by the rotating shaft to push the raw material in one direction;

[0011] A stirring blade, wherein a plurality of the stirring blades are arranged around the rotating shaft inside the tank body, and a cutting edge is installed on the edge of the stirring blade away from the rotating shaft. A spiral component is vertically arranged between two adjacent stirring blades. The heat-sensitive element absorbs the heat generated by the fermentation of the raw materials and causes expansion, which is used to push the cutting edge to adhere to the wall inside the tank body, and the spiral component is driven to rotate against the wall and push the raw materials in the opposite direction relative to the blades;

[0012] An oxygen pipe extends from the bottom into the tank body, and the oxygen flow ejected by the oxygen pipe is dispersed by the spiral component, and the stirring blade is used to stir the oxygen flow to achieve diffusion inside the tank body;

[0013] The cooling water tank is annular and covers the tank body. The cooling water tank absorbs the heat of the tank body, cools the heat-sensitive component, and then pulls the stirring blade away from the inner wall of the tank body, opening the gap for disinfection.

[0014] As a preferred embodiment, the heat-sensitive element includes an ether solution layer and an airtight plug arranged radially along the rotating shaft. The ether solution layer absorbs heat and expands to push the stirring blade to adhere to the wall inside the tank. The end of the airtight plug away from the ether solution layer is in a rod-shaped manner to support the adjacent stirring blade.

[0015] After the ether solution layer is cooled, it is used to pull the stirring blade away from the inner wall of the tank.

[0016] As a preferred solution, the outer portion of the ether solution layer is covered with an alloy cylinder, the alloy cylinders are arranged radially along the rotation axis and the openings of the alloy cylinders cover the rod of the airtight plug, and the inner portion of the alloy cylinders is further provided with a liquid sealing layer and an inert circular plate for separating the ether solution layer;

[0017] Wherein, when the airtight plug, the liquid sealing layer and the inert circular plate support the stirring blade, the ether solution layer is blocked inside the alloy cylinder.

[0018] As a preferred solution, a concave plate fixedly connected to the stem of the airtight plug is provided on the outside of the rotating shaft, and diagonal braces are fixed between the opening of the concave plate and one side of two adjacent stirring blades;

[0019] The concave plate and the diagonal support open a gap between two adjacent stirring blades, which is used for the spiral assembly to rotate and separate the blades.

[0020] As a preferred solution, the spiral assembly includes a spiral piece vertically arranged between two stirring blades, and coaxial metal rings are welded to both ends of the spiral piece;

[0021] The spiral blades scrape the attached objects after being attached to the wall along with the stirring blades, and the spiral blades are rotated by the friction between the metal ring and the inner wall of the tank body. The spiral blades rotate on themselves and stir the raw materials in the opposite direction relative to the blades.

[0022] As a preferred solution, both ends of the concave plate are welded with a rotating seat, one end of each of the two rotating seats extends between two adjacent stirring blades and a vertical shaft is rotatably installed between the ends, and the vertical shaft axially penetrates the spiral blade along the axial direction;

[0023] The rotating seat and the vertical shaft support the spiral piece to be suspended in the air and separated from the stirring blade, leaving a space for disinfection.

[0024] As a preferred solution, a silicone ring is nested on the outside of the metal ring. The deformation of the silicone ring itself is used to buffer the adhesion action between the metal ring and the inner wall of the tank. The outer surface of the silicone ring is embedded with ceramic balls for friction with the inner wall of the tank.

[0025] As a preferred solution, the driving element includes a reduction motor and a sterile shaft seal sleeved on the output end of the reduction motor, and the output end of the reduction motor is used to extend into the tank body to rotate the rotating shaft.

[0026] As a preferred solution, a feeding hatch cover and an observation window are arranged at intervals on the upper surface of the tank body. The feeding hatch cover is used to add raw materials and inoculate microorganisms. The lower surface of the tank body is connected downwardly with a discharge valve and a discharge pipe in sequence. The outside of the discharge pipe is connected to a steam pipe for tank disinfection.

[0027] As a preferred solution, the lower edge of the cooling water tank is connected to a cooling water valve, a temperature sensor in contact with the outer surface of the tank body is inserted inside the cooling water tank, and the upper edge of the cooling water tank is connected to a drain pipe.

[0028] The technical effects achieved by the present invention are:

[0029] The present invention activates a driving element to rotate the rotating shaft, blades, stirring blades, and spiral assembly to achieve uniform mixing. Heat generated during fermentation causes the heat-sensitive element to expand, pushing the stirring blades, cutting edges, and spiral assembly radially along the rotating shaft to adhere to the wall. This stirring action scrapes off debris attached to the inner wall while stirring the mixture, and drives the spiral assembly to rotate vertically to push the mixture. This multi-layered stirring process facilitates enhanced mixing. After cooling, the heat-sensitive element cools and contracts, driving the stirring blades, cutting edges, and spiral assembly away from the inner wall of the tank, freeing them up to allow sufficient space for disinfection, minimizing residual microorganisms, and effectively coordinating inner wall scraping and cleaning with internal disinfection.

[0030] The heat generated by the present invention heats the ether solution layer to 30-37°C during the fermentation reaction of the mixture, causing expansion and outward squeezing of the airtight plug, thereby pushing the stirring blades horizontally. There is no need to build pipelines into the tank body, and the use of structures such as threaded holes is avoided, thereby preventing the increase of microbial residues in hidden areas during the disinfection process.

[0031] The present invention comprises an airtight plug and an alloy cylinder body to form a closed piston structure, with an ether solution layer, a liquid sealing layer and an inert circular plate built in. The structure can expand when heated to push a stirring blade in a straight line, and contract after cooling to pull the stirring blade away from the wall. In addition, the unique airtight plug, liquid sealing layer and inert circular plate respectively form three layers of sealing on the outside of the ether solution layer, thereby reducing leakage and improving the safety of the mixture.

[0032] In the present invention, as the spiral piece moves laterally, the spiral piece drives the metal ring to stick to the inner wall of the tank body. On the one hand, it revolves with the rotating shaft to stir the mixture circumferentially. On the other hand, the friction between the metal ring and the inner wall drives the spiral piece to rotate. The spiral piece stirs the mixture downward, while the blade stirs the mixture upward, thereby achieving stirring of mixtures at different depths and avoiding stratification of the mixture. At the same time, the spiral piece is subjected to the upward reaction of the mixture, which can reduce the vertical load of the closed piston structure. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] Figure 1 This is a front view of an integrated biopharmaceutical raw material fermentation processing device according to an embodiment of the present invention;

[0034] Figure 2 This is a cross-sectional view of an integrated biopharmaceutical raw material fermentation processing device according to an embodiment of the present invention;

[0035] Figure 3 This is a front view of the driving element mounting rotation axis in an embodiment of the present invention;

[0036] Figure 4 is a top view of the driving element mounting rotation axis in an embodiment of the present invention;

[0037] Figure 5 This is a front view of a heat-sensitive member supporting a stirring blade in an embodiment of the present invention;

[0038] Figure 6 is a cross-sectional view of a thermal-sensitive member in an embodiment of the present invention;

[0039] Figure 7 is a front view of a spiral sheet in an embodiment of the present invention;

[0040] Figure 8 2 is a front view of a metal ring in an embodiment of the present invention.

[0041] In the accompanying drawings, the components represented by the reference numerals are as follows:

[0042] 1. Tank body; 101. Support legs; 2. Rotating shaft; 201. Blades; 3. Stirring blades; 301. Cutting edge; 4. Oxygen pipe; 5. Cooling water tank; 6. Ether solution layer; 7. Airtight plug; 8. Alloy cylinder; 9. Liquid seal layer; 10. Inert circular plate; 11. Concave plate; 12. Diagonal brace; 13. Spiral blade; 14. Metal ring; 15. Vertical axis; 16. Rotating seat; 17. Silicone ring; 18. Ceramic ball; 19. Sterile shaft seal; 20. Reducer motor; 21. Feeding hatch; 22. Observation window; 23. Discharge valve; 24. Discharge pipe; 25. Steam pipe; 26. Cooling water valve; 27. Temperature sensor; 28. Drain pipe. DETAILED DESCRIPTION

[0043] In order to make the purpose and advantages of the present invention more clearly understood, the present invention is described in detail below with reference to the following examples. It should be understood that the following text is only used to describe one or more specific embodiments of the present invention and does not strictly limit the scope of protection of the present invention.

[0044] like Figures 1-8 As shown, a biopharmaceutical integrated raw material fermentation processing device includes a tank body 1, a rotating shaft 2 and stirring blades 3, an oxygen tube 4 and a cooling water tank 5. Three legs 101 are welded to the lower edge of the tank body 1 for grounding. The tank body 1 is controlled by an external industrial computer. Before the tank body 1 is put into use, its inner tube and various pipes and valves are thoroughly disinfected. The rotating shaft 2 is axially arranged inside the tank body 1, and multiple stirring blades 3 are arranged around the rotating shaft 2 inside the tank body 1. A cutting edge 301 is installed on the edge of the stirring blade 3 away from the rotating shaft 2, and a spiral component is vertically arranged between two adjacent stirring blades 3. The rotating shaft 2 is sequentially provided with a driving element and a blade 201 from top to bottom. After the driving element is started, the blade 201 is pushed upward by the unidirectional rotation of the rotating shaft 2, the cutting edge 301 scrapes off attachments against the wall, and the spiral component rotates against the wall and pushes the raw material in the opposite direction relative to the blade 201.

[0045] Refer to the attached Figure 1 and Figure 2 A feeding hatch cover 21 is provided on the upper surface of the tank body 1. The feeding hatch cover 21 is closed by six hand wheels and can be opened manually for adding raw materials and inoculating microorganisms. It can be closed after adding enough materials.

[0046] Refer to the attached Figure 1 and Figure 2The driving element includes a reduction motor 20 and a sterile shaft seal 19 mounted on the output end of the reduction motor 20. The reduction motor 20 can select the DSZRF27-58 / Y2711-4E-Z-IF-M1 reducer motor. The output end of the reduction motor 20 is used to extend into the tank body 1 to rotate the rotating shaft 2. The reduction motor 20 is connected to the industrial computer through a relay signal. After starting, it drives the rotating shaft 2 to rotate the blades 201 and the stirring blades 3. The gear set is used for transmission, and the differential ratio is adjusted to the set range. The sterile shaft seal 19 is used to seal the gap between the tank body 1 and the rotating shaft 2 to improve the sealing during stirring.

[0047] Furthermore, the number of blades 201 is four, and the number of stirring blades 3 can be set to six, with every two stirring blades 3 forming a pair and arranged at intervals. When stirring the raw materials, the load on the rotating shaft 2 is small, making it convenient for the driving element to start rotating the rotating shaft 2 faster.

[0048] Furthermore, the number of stirring blades 3 can be set to eight, with two stirring blades 3 forming a pair and arranged at intervals. Compared with six stirring blades 3, the stirring area is larger. Although the load of the rotating shaft 2 is increased, the stirring efficiency can be improved.

[0049] As an optional embodiment, a heat-sensitive component is provided on the rotating shaft 2, which absorbs the heat generated by the fermentation of the raw materials and causes expansion, and is used to push the cutting edge 301 to adhere to the wall inside the tank body 1, and can link the spiral component to rotate against the wall and push the raw materials in the opposite direction relative to the blade 201.

[0050] Refer to the attached Figure 4 、 Figure 5 and Figure 6 The heat-sensitive component includes an ether solution layer 6 and an airtight plug 7 arranged radially along the rotating shaft 2. The ether solution layer 6 absorbs heat and expands to push the stirring blade 3 to adhere to the wall inside the tank body 1. The end of the airtight plug 7 away from the ether solution layer 6 is rod-shaped to support the adjacent stirring blade 3, and the other end is plunger-shaped to contact the ether solution layer 6 with a larger area. The plunger is sealed with the ether solution layer 6 outside the rotating shaft 2. While the mixture is fermenting, the heat generated heats the ether solution layer 6 to 30-37°C, causing expansion to squeeze the airtight plug 7 outward, thereby pushing the stirring blade 3 horizontally. There is no need to build a pipeline into the tank body 1, and it also avoids the use of structures such as threaded holes, which prevents the increase of hidden areas during the disinfection process and causes microbial residues.

[0051] Furthermore, after the ether solution layer 6 is cooled, it is used to pull the stirring blade 3 away from the inner wall of the tank body 1 and is separated by the airtight plug 7 so as not to contact the stirring blade 3 in diameter.

[0052] Refer to the attached Figure 3 、 Figure 5 and Figure 6The outside of the ether solution layer 6 is covered with an alloy cylinder body 8. The alloy cylinder bodies 8 are arranged radially along the rotating shaft 2 and the openings are covered with the rod of the airtight plug 7. The end faces of the alloy cylinder bodies 8 are welded to the outside of the rotating shaft 2. The inside of the alloy cylinder body 8 is also provided with a liquid sealing layer 9 and an inert circular plate 10 for separating the ether solution layer 6. The liquid sealing layer 9 can be made of pure water, which is weakly soluble in ether. The inert circular plate 10 can be made of tin-nickel alloy, which has good mechanical structural properties. The airtight plug 7 and the alloy cylinder body 8 form a closed piston structure with built-in ether solution layer 6, liquid sealing layer 9 and inert circular plate 10. It can expand in a straight line when heated and push the stirring blade 3. It shrinks after cooling and can pull the stirring blade 3 away from the wall. The unique airtight plug 7, liquid sealing layer 9 and inert circular plate 10 respectively form three layers of sealing on the outside of the ether solution layer 6 to reduce leakage and improve the safety of the mixture.

[0053] Furthermore, when the airtight plug 7, the liquid sealing layer 9 and the inert circular plate 10 support the stirring blade 3, the ether solution layer 6 is sealed inside the alloy cylinder 8. In the cooled state, the length of the ether solution layer 6 is less than half the depth of the alloy cylinder 8, leaving enough space for the airtight plug 7 to move.

[0054] The oxygen pipe 4 extends into the tank body 1 from the bottom, and the air inlet of the oxygen pipe 4 is connected to the oxygen tank. The oxygen flow can be sprayed out by opening the oxygen tank valve and the oxygen pipe 4. The oxygen flow sprayed by the oxygen pipe 4 is broken up by the spiral component, and the stirring of the stirring blade 3 is combined to realize the diffusion of the oxygen flow inside the tank body 1. The upper surface of the tank body 1 is also connected to an exhaust pipe, and the exhaust gas is collected by the drainage method.

[0055] During this process, oxygen flow is introduced to ensure that the bacteria have sufficient nutrients to reproduce, and the culture is carried out at a set temperature. The driving unit is started to rotate the rotating shaft 2, blades 201, stirring blades 3 and spiral components to stir the mixture so that it is evenly mixed. The cutting edge 301 can scrape off attachments along the inner wall of the tank body 1.

[0056] In particular, the air outlet of the oxygen tube 4 is directed toward the path of the spiral assembly rotating with the stirring blade 3. The spiral assembly can diffuse the oxygen flow while stirring, so that as many bacteria as possible can be exposed to the oxygen flow, and the excess exhaust gas from the tank body 1 flows out from the exhaust pipe.

[0057] Heat is generated during fermentation, and circulating cold water is pumped into the cooling water tank 5 to take away the heat of the tank 1 and continuously cool it down to prevent the mixture from being overheated and causing the bacteria to be inactivated.

[0058] Refer to the attached Figure 1 and Figure 2 An observation window 22 is also provided on the upper surface of the tank body 1. Transparent glass is embedded in the observation window 22. The inner surface of the transparent glass is coated with a non-stick coating. The mixture is stirred at a constant speed for a certain period of time, and the state of the mixture can be viewed through the observation window 22.

[0059] Moreover, the outer surface of the closed piston structure is smooth without obvious pits, gaps, etc., and it is not easy for microorganisms to remain. The three-layer sealing can also prevent disinfection gas from entering the alloy cylinder 8 and avoid contact with the ether solution layer 6.

[0060] Refer to the attached Figure 3 、 Figure 4 and Figure 5 A concave plate 11 is provided on the outside of the rotating shaft 2 and is fixedly connected to the rod of the airtight plug 7. An oblique support 12 is fixed between the opening of the concave plate 11 and one side of two adjacent stirring blades 3. The concave plate 11 and the oblique support 12 are used to support the stirring blade 3 and the closed piston structure. Each concave plate 11 supports two stirring blades 3, making it convenient for the two stirring blades 3 to be suspended in the air and surround the spiral assembly from both sides.

[0061] Furthermore, the concave plate 11 and the diagonal support 12 open the interval between two adjacent stirring blades 3 for the rotation of the spiral assembly and for separating the blades 201 for the flow of the mixture and for facilitating the flow of the sterilizing gas or liquid.

[0062] Refer to the attached Figure 4 、 Figure 5 and Figure 7 The spiral assembly includes a spiral piece 13 vertically arranged between the two stirring blades 3, and a coaxial metal ring 14 is welded at both ends of the spiral piece 13. As the spiral piece 13 moves horizontally, the spiral piece 13 drives the metal ring 14 to stick to the inner wall of the tank body 1. On the one hand, it revolves with the rotating shaft 2 to stir the mixture circumferentially. On the other hand, the friction between the metal ring 14 and the inner wall drives the spiral piece 13 to rotate. In this embodiment, the spiral piece 13 stirs the mixture downward, while the blade 201 stirs the mixture upward, so as to achieve stirring of mixtures of different depths and avoid stratification of the mixture. At the same time, the spiral piece 13 is subjected to the upward reaction of the mixture, which can reduce the vertical load of the closed piston structure.

[0063] In particular, the spiral blades 13 scrape the attached matter after the stirring blades 3 adhere to the wall, reducing the thickness of the attached matter. The scraping amount of each cutting edge 301 decreases, thereby alleviating the workload of the cutting edge 301.

[0064] Refer to the attached Figure 3 、 Figure 4 and Figure 7 , both ends of the concave plate 11 are welded with a rotating seat 16, one end of the two rotating seats 16 extends between the two adjacent stirring blades 3 and a vertical shaft 15 is rotatably installed inside the one end. The vertical shaft 15 axially penetrates the spiral piece 13. The rotating seat 16 is used to support the vertical shaft 15. The suspended spiral piece 13 is separated from the two stirring blades 3 to prevent mutual friction. It is also convenient to keep the spiral piece 13 parallel to the inner wall of the tank body 1 to avoid uneven wear after sticking to the wall.

[0065] Furthermore, the spiral blade 13 is suspended in the air by supporting the rotating base 16 and the vertical shaft 15, and is separated from the stirring blade 3 to maintain a large gap, leaving a space for disinfection and reducing the residual zone of microorganisms.

[0066] As an optional embodiment, a silicone ring 17 is nested on the outside of the metal ring 14. The deformation of the silicone ring 17 itself is used to buffer the adhesion action of the metal ring 14 to the inner wall of the tank body 1. The outer surface of the silicone ring 17 is embedded with a ceramic ball 18 for friction with the inner wall of the tank body 1.

[0067] Furthermore, the silicone ring 17 partially extends out of the metal ring 14 and can be deformed as the stirring blade 3 adheres to the wall to cushion the metal ring 14 and avoid hard collisions with the inner wall of the tank body 1 to form pits.

[0068] Furthermore, the ceramic balls 18 may be made of silicon nitride ceramics or silicon carbide ceramics, which increases the friction with the inner wall of the tank body 1 and prevents slipping, so that the metal ring 14 can smoothly drive the spiral piece 13 to rotate.

[0069] Refer to the attached Figure 1 and Figure 2 The cooling water tank 5 is filled with circulating cold water, which can dissipate heat continuously. The cooling water tank 5 is annular and covers the tank body 1. The cooling water tank 5 absorbs the heat of the tank body 1 and cools the mixture in the tank body 1. The lower edge of the cooling water tank 5 is connected to a cooling water valve 26. The water inlet of the cooling water valve 26 is connected to the tap water source. A temperature sensor 27 in contact with the outer surface of the tank body 1 is inserted into the cooling water tank 5. The temperature sensor 27 is connected to the industrial computer through a signal converter. The upper edge of the cooling water tank 5 is connected to a drain pipe 28. When the cooling water tank 5 cools the tank body 1, the tap water source and the cooling water valve 26 are opened to continuously supply tap water to the cooling water tank 5 to take away the heat of the tank body 1. The tail water is discharged from the drain pipe 28, and the temperature sensor 27 monitors the temperature signal of the tank body 1 in real time. If the temperature is too low, the amount of tap water is reduced, or if the temperature is too high, the amount of tap water is increased.

[0070] The cooling water tank 5 pumps circulating cold water to continuously reduce the temperature to prevent the mixture from overheating and causing the inactivation of the bacteria.

[0071] The lower surface of the tank body 1 is connected downward in sequence with a discharge valve 23 and a discharge pipe 24. The outside of the discharge pipe 24 is connected to a steam pipe 25 for disinfecting the tank body 1. The steam pipe 25 is connected in sequence with a high-temperature resistant air pump and a steam source. The discharge valve 23 can be opened and the oxygen pipe 4 can be closed to discharge the mixed material along the discharge pipe 24.

[0072] After emptying, the steam source is turned on, and high-temperature steam is continuously introduced into the interior of the tank body 1 through the steam pipe 25 to kill the remaining microorganisms by the high temperature and perform disinfection.

[0073] Among them, the cooling water tank 5 is annular and covers the tank body 1. The cooling water tank 5 absorbs the heat of the tank body 1, cools the heat-sensitive component, and drives the stirring blade 3, the blade 301 and the spiral component to separate from the inner wall of the tank body 1, opening the spacing to leave enough space for disinfection and minimize the residual microorganisms.

[0074] The disinfection steps involved in this embodiment are as follows:

[0075] Step 1: Emptying: Empty all the materials in the fermentation tank;

[0076] Step 2: Rinse: Use plenty of clean water to preliminarily rinse the inside of the fermentation tank to remove residual materials and some pollutants;

[0077] Step 3: Alkali washing: prepare an alkaline solution (such as sodium hydroxide solution) of appropriate concentration, inject it into the fermentation tank, soak it for a period of time, turn on the stirring device, etc., so that the alkaline solution can fully contact all parts of the tank to remove pollutants such as grease and protein, and then discharge the alkaline solution;

[0078] Step 4: Pickling: Use an acidic solution of appropriate concentration (such as dilute hydrochloric acid) to clean and further remove some stubborn dirt and possible residual alkaline substances, soak and stir, and then discharge;

[0079] Step 5. Disinfection: Chemical disinfectants (such as hydrogen peroxide solution, chlorine-containing disinfectants, etc.) can be used to disinfect the fermentation tank to ensure that residual bacteria and other microorganisms are killed. After disinfection, keep it for a period of time before discharging;

[0080] Step 6: Rinse with clean water: Rinse the fermentation tank again with plenty of clean water to remove residual disinfectant and other impurities

[0081] The foregoing is merely a preferred embodiment of the present invention. It should be noted that those skilled in the art may make various improvements and modifications without departing from the principles of the present invention, and such improvements and modifications are also within the scope of protection of the present invention. Structures, devices, and operating methods not specifically described or explained herein shall, unless otherwise specified or limited, be implemented in accordance with conventional means in the art.

Claims

1. A biopharmaceutical integrated raw material fermentation processing device, comprising a tank body (1), characterized in that: Also includes: A rotating shaft (2), the rotating shaft (2) being axially arranged inside the tank body (1), the rotating shaft (2) being provided with a driving element, a heat-sensitive element, and a blade (201) in order from top to bottom, the blade (201) pushing the raw material in a unidirectional manner when being rotated by the rotating shaft (2); A stirring blade (3), wherein a plurality of the stirring blades (3) are arranged around the rotating shaft (2) inside the tank body (1), and a cutting edge (301) is installed on the edge of the stirring blade (3) away from the rotating shaft (2), and a spiral component is vertically arranged between two adjacent stirring blades (3), and the heat-sensitive element absorbs the heat generated by the fermentation of the raw materials to cause expansion, and is used to push the cutting edge (301) to adhere to the wall inside the tank body (1), and the spiral component is linked to rotate against the wall and push the raw materials in the opposite direction relative to the blade (201); An oxygen pipe (4) extends from the bottom into the tank body (1), and the oxygen pipe (4) sprays an oxygen flow which is dispersed by a spiral assembly and cooperates with the stirring blade (3) to achieve diffusion of the oxygen flow inside the tank body (1); A cooling water tank (5) is provided, wherein the cooling water tank (5) is annularly enclosing the tank body (1). The cooling water tank (5) absorbs heat from the tank body (1), cools the heat-sensitive element, and pulls the stirring blade (3) away from the inner wall of the tank body (1), thereby opening the gap for disinfection.

2. The integrated biopharmaceutical raw material fermentation processing device according to claim 1, characterized in that: The heat-sensitive element comprises an ether solution layer (6) and an airtight plug (7) radially arranged along the rotating shaft (2); the ether solution layer (6) absorbs heat and expands to push the stirring blade (3) against the wall inside the tank (1); and the end of the airtight plug (7) away from the ether solution layer (6) is in a rod-like shape to support the adjacent stirring blade (3); After the ether solution layer (6) is cooled, it is used to pull the stirring blade (3) away from the inner wall of the tank body (1).

3. The integrated biopharmaceutical raw material fermentation processing device according to claim 2, characterized in that: The exterior of the ether solution layer (6) is provided with an alloy cylinder (8), the alloy cylinder (8) is radially arranged along the rotating shaft (2) and the opening thereof covers the rod of the airtight plug (7), and the interior of the alloy cylinder (8) is further provided with a liquid sealing layer (9) and an inert circular plate (10) for separating the ether solution layer (6); When the airtight plug (7), the liquid sealing layer (9) and the inert circular plate (10) support the stirring blade (3), the ether solution layer (6) is blocked inside the alloy cylinder (8).

4. The integrated biopharmaceutical raw material fermentation processing device according to claim 2, characterized in that: A concave plate (11) fixedly connected to the rod of the airtight plug (7) is provided on the outside of the rotating shaft (2), and a diagonal brace (12) is fixed between the opening of the concave plate (11) and one side of two adjacent stirring blades (3); The concave plate (11) and the diagonal support (12) open the interval between two adjacent stirring blades (3) for the spiral assembly to rotate and separate the blades (201).

5. The integrated biopharmaceutical raw material fermentation processing device according to claim 1, characterized in that: The spiral assembly comprises a spiral piece (13) vertically arranged between two stirring blades (3), and coaxial metal rings (14) are welded to both ends of the spiral piece (13); The spiral blade (13) scrapes the attached matter after adhering to the wall with the stirring blade (3), and rotates the spiral blade (13) through the friction between the metal ring (14) and the inner wall of the tank body (1). The spiral blade (13) rotates on its own and stirs the raw materials in the opposite direction relative to the blade (201).

6. The integrated biopharmaceutical raw material fermentation processing device according to claim 4, characterized in that: Both ends of the concave plate (11) are welded with a rotating seat (16), one end of each of the two rotating seats (16) extends between two adjacent stirring blades (3) and a vertical shaft (15) is rotatably installed between the ends, and the vertical shaft (15) is axially fixed inside, and the vertical shaft (15) axially penetrates the spiral blade (13); The rotating seat (16) and the vertical shaft (15) support the spiral piece (13) to be suspended in the air and separated from the stirring blade (3) to leave a spacing for disinfection.

7. The integrated biopharmaceutical raw material fermentation processing device according to claim 5, characterized in that: A silicone ring (17) is nested on the outside of the metal ring (14), and the deformation of the silicone ring (17) itself is used to buffer the action of the metal ring (14) adhering to the inner wall of the tank body (1). The outer surface of the silicone ring (17) is embedded with a ceramic ball (18) for friction with the inner wall of the tank body (1).

8. The integrated biopharmaceutical raw material fermentation processing device according to claim 1, characterized in that: The driving element comprises a reduction motor (20) and a sterile shaft seal (19) sleeved on an output end of the reduction motor (20), wherein the output end of the reduction motor (20) is used to extend into the tank body (1) to rotate the rotating shaft (2).

9. The integrated biopharmaceutical raw material fermentation processing device according to claim 1, characterized in that: The upper surface of the tank body (1) is provided with a feeding hatch cover (21) and an observation window (22) at intervals. The feeding hatch cover (21) is used for adding raw materials and inoculating microorganisms. The lower surface of the tank body (1) is connected downwardly with a discharge valve (23) and a discharge pipe (24) in sequence. The outer side of the discharge pipe (24) is connected with a steam pipe (25) for sterilizing the tank body (1).

10. The integrated biopharmaceutical raw material fermentation processing device according to claim 1, characterized in that: The lower edge of the cooling water tank (5) is connected to a cooling water valve (26), a temperature sensor (27) in contact with the outer surface of the tank body (1) is inserted into the cooling water tank (5), and the upper edge of the cooling water tank (5) is connected to a drain pipe (28).

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