Microbial strain survival rate activity detection equipment and method

Abstract

The invention belongs to the technical field of biology, and discloses microbial strain survival rate activity detection equipment and method.The microbial strain survival rate activity detection equipment comprises an equipment bottom plate, a temperature control mechanism is slidably mounted in the equipment bottom plate, a clamping groove is formed in the top face of the equipment bottom plate, and a heat conduction box is clamped to the inner side of the clamping groove; a culture medium flat plate is arranged in the heat conduction box, a mounting ring is fixedly connected to the outer side of the top of the clamping groove and located at the top of the equipment bottom plate, a fixing block is fixedly mounted on one side of the heat conduction box and located at the top of the equipment base, a groove is formed in one side of the mounting block, and a driving structure is arranged in the groove. Through cooperative use of the droppers, the driving structure, the mounting block, the connecting block, the clamping structure and the fixing plate, the droppers can be conveniently replaced at a time, the droppers do not need to be additionally taken for sampling, the droppers do not need to be replaced for multiple times, use is convenient, and the working efficiency can be improved.

Description

Technical Field

[0001] The present invention belongs to the technical field of biotechnology, and in particular relates to a device and method for detecting the survival rate and activity of microbial strains. Background Art

[0002] The preservation of biological strains is a crucial task. Microbial strains of research value in scientific research and testing need to be preserved, and many specialized institutions specialize in this area. Microbial strains are preserved using various methods and using different protective agents. After preservation, samples are collected periodically to measure their survival and activity. This information is then used to determine whether the strains can be preserved further or require rejuvenation and re-preservation.

[0003] For example, the Chinese patent application number CN2021227422163 discloses a microbial strain survival rate and activity detection device, including a device base plate, a temperature control mechanism is slidably installed on the inner side of the device base plate, the temperature control mechanism includes a mounting frame, and a Peltier fixedly mounted on the inner side of the mounting frame. The Peltiers are evenly distributed, and the cooling surface and heating surface of adjacent Peltiers are staggered. A number of temperature dissipation holes are provided on the bottom surface of the device base plate, and the number of temperature dissipation holes corresponds to the bottom surface of the Peltier. During use, the temperature can be controlled by a number of staggered Peltiers, and the cooling surface and heating surface between adjacent Peltiers are staggered, so that the culture medium plate can ensure that the inner side of the culture medium plate is at a suitable temperature and humidity before use, thereby avoiding the phenomenon of water droplets on the colonies in the inner cavity of the culture medium plate, which in turn affects the measurement of the colonies and improves the accuracy of the detection.

[0004] However, during use, after each sampling and dilution, the used pipette can be placed inside the placement box, and another sterile pipette is used for sampling. The pipette needs to be replaced many times, which is troublesome to use and is not conducive to improving the efficiency of detection. Summary of the Invention

[0005] The present invention aims to solve the problem in the prior art that after each sampling and dilution, the used pipette can be placed inside the placement box, and another sterile pipette is taken for sampling, which requires multiple replacement of pipettes, is cumbersome to use and is not conducive to improving the efficiency of detection. The present invention proposes the following technical solution: a microbial strain survival rate and activity detection device, comprising a device base plate, a temperature control mechanism is slidably installed inside the device base plate, a clamping groove is provided on the top surface of the device base plate, a heat conduction box is clamped inside the clamping groove, a culture medium plate is provided inside the heat conduction box, a mounting ring is fixedly connected to the top of the device base plate on the outside of the top of the clamping groove, a fixing block is fixedly installed on one side of the heat conduction box and on the top of the device base, a groove is provided on one side of the mounting block, a driving structure is provided inside the groove, a mounting block is provided on one side of the driving structure, a connecting block is fixedly installed in the middle position of the mounting block, a clamping structure is provided inside the connecting block, and an insertion interface is provided on the top of the connecting block, a dropper is provided inside the insertion interface, a fixing plate is provided at the bottom end of the dropper and on one side of the fixing block, and a fixing device is provided inside the fixing plate.

[0006] As a preferred embodiment of the above technical solution, mounting grooves are provided on the front and rear sides of the mounting ring, a slot is provided at the bottom of the mounting groove, an insert is inserted into the inside of the slot, a compression spring is fixedly connected to the top of the insert, a block is fixedly connected to the bottom of the compression spring, and one side of the block is fixedly connected to the outer surface of the heat conduction box.

[0007] As a preferred embodiment of the above technical solution, the driving structure includes a motor, the bottom of the motor is fixedly connected to the bottom inner wall of the groove, the output end of the motor is fixedly connected to a rotating shaft, the top of the rotating shaft is rotatably connected to the top inner wall of the groove, and the top outer surface of the rotating shaft is sleeved with a first gear.

[0008] As a preferred embodiment of the above technical solution, a slide is fixedly connected to the bottom of the mounting block, a slide groove is provided on one side of the groove, one side of the slide is slidably connected to the slide groove, the middle part of the slide is fixedly socketed with the outer surface of the rotating shaft, a through hole is provided in the middle part of the slide, and the rotating shaft extends upward through the through hole.

[0009] As a preferred embodiment of the above technical solution, the clamping structure includes a second gear, a screw rod is fixedly connected to the middle part of the second gear, a first clamping plate is fixedly sleeved on the outer surface of one end of the screw rod, and a second clamping plate is fixedly sleeved on the outer surface of the other end of the screw rod, the inner walls of the first clamping plate and the second clamping plate are fixedly connected to the buffer spring, and one end of the buffer spring is fixedly connected to the clamping block.

[0010] As a preferred embodiment of the above technical solution, movable openings are provided on the outer surfaces of both sides of the connecting block, the second gear is located outside the mounting block, one end of the screw rod passes through the first clamping block and is rotatably connected to the second clamping block and the inner wall of the mounting block, the second clamping plate and the second clamping plate are located inside the connecting block, the first clamping plate and the second clamping plate are both arc-shaped, and both ends of the first clamping plate are provided with sockets, and both ends of the second clamping plate are connected to the sockets.

[0011] As a preferred embodiment of the above technical solution, the clamping block is clamped with the dropper, the clamping block is arc-shaped, and is distributed in a central ring array of the connecting block. A rubber pad is provided inside the clamping block, and the rubber pad fits the outer surface of the dropper.

[0012] As a preferred embodiment of the above technical solution, a moving block is fixedly connected to one side of the fixed plate, one end of the moving block is slidably connected to the slide groove, the middle part of the moving block is fixedly sleeved with the rotating shaft, a fixing hole is provided on the top of the fixed plate, a fixing groove is provided inside the fixed plate, and the fixing device is located inside the fixing groove and on both sides of the dropper.

[0013] As a preferred embodiment of the above technical solution, the fixing device includes a support ring, the top surface of the support ring is fixedly connected to the top inner wall of the fixed groove, two movable grooves are opened on the top of the support ring, the internal movability of the movable groove is connected with a movable rod, the bottom of the movable rod is fixedly connected with a reset spring, the bottom of the reset spring is fixedly connected with a movable plate, one end of the movable plate is fixedly connected with a support plate, the bottom of one end of the movable plate is hinged with a support rod, the bottom of the support rod is fixedly connected to the bottom inner wall of the fixed groove, and a telescopic rod is fixedly connected to one side of the support rod and located at the bottom of the movable plate.

[0014] The present invention also provides a method for using the above-mentioned microbial strain survival rate activity detection device, which comprises the following steps:

[0015] Step 1: First, pour the diluted bacterial solution into the dropper. The bacterial solution falls from the dropper onto the culture medium plate. The temperature control mechanism increases the temperature of the heat conduction box. Hot air is transferred to the inner side of the culture medium plate through the heat conduction box, drying the water flow inside the culture medium plate. When the dryness reaches a certain level, the temperature control mechanism is turned off.

[0016] Step 2: Then start the motor, which drives the rotating shaft and the first gear to rotate, and at the same time drives the movable plate and the slide plate to move upward along the slide slot, so that the fixed plate and the mounting block move upward with the dropper, and the mounting block moves upward to drive the second gear to move upward. When the second gear moves and engages with the first gear, the first gear rotates to drive the second gear to rotate, and the second gear rotates to drive the screw rod to rotate, so that the first clamping plate and the second clamping plate move toward the two ends of the screw rod, driving the clamping block away from the dropper, so that the dropper is no longer clamped;

[0017] Step 3: Then the bottom end of the dropper moves toward the inside of the fixed plate, so that the bottom end of the dropper supports the moving rod on the top of the extrusion ring, causing the moving rod to move downward in the moving groove. The return spring is compressed, causing one end of the moving plate to deflect downward, and the other end drives the abutment plate to deflect upward, causing the telescopic rod to extend and retract. While the dropper moves downward, the low rod and the moving plate support the bottom end of the dropper, and then the dropper is removed from the mounting block and the fixed plate.

[0018] Step 4: After the final static state is completed, pull the block upwards so that the block drives the compression spring to stretch, separating the plug from the slot, thereby removing the culture medium plate inside the thermal box from the bottom plate of the device, replacing it with a new culture medium plate, and conducting the next test.

[0019] The beneficial effects of the present invention are:

[0020] 1. Through the coordinated use of the dropper, the driving structure, the mounting block, the connecting block, the clamping structure and the fixing plate, after the dropper has dripped the bacterial solution into the culture medium plate, the driving structure causes the mounting block, the fixing block and the connecting block to move upward, so that the dropper moves upward. At the same time, the driving structure drives the clamping structure so that the dropper is no longer clamped on the dropper, which is convenient for replacing multiple droppers at one time. There is no need to take another dropper for sampling, and there is no need to replace the dropper multiple times. It is convenient to use and is conducive to improving work efficiency.

[0021] 2. Through the coordinated use of the dropper, fixing plate, fixing groove and fixing device, when the clamping structure no longer clamps the dropper, the dropper moves downward. When the dropper moves downward, the bottom end squeezes the fixing device inside the fixing groove, so that the fixing device supports the bottom of the dropper, preventing the dropper from falling and facilitating the replacement of the dropper. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 shows a schematic diagram of the overall structure of an embodiment of the present invention;

[0023] Figure 2 It shows the internal structure of the bottom plate of the device according to the embodiment of the present invention;

[0024] Figure 3 Shows the internal structure of the mounting block according to an embodiment of the present invention;

[0025] Figure 4 shows the internal structure of the connection block according to an embodiment of the present invention;

[0026] Figure 5 Shows a structural diagram of a clamping device according to an embodiment of the present invention;

[0027] Figure 6 Shows the internal structure of the fixing plate according to an embodiment of the present invention;

[0028] Figure 7 shows a structural diagram of a fixing device according to an embodiment of the present invention;

[0029] Figure 8 A flow chart of a method according to an embodiment of the present invention is shown.

[0030] In the figure: 1. Equipment base plate; 2. Temperature control mechanism; 3. Heat conduction box; 4. Culture medium plate; 5. Mounting ring; 6. Fixed block; 7. Mounting block; 8. Connecting block; 9. Clamping structure; 91. Second gear; 92. Screw; 93. First clamping plate; 94. Second clamping plate; 95. Buffer spring; 96. Clamping block; 10. Dropper; 11. Fixed plate; 12. Slot; 13. Insert block; 14. Compression spring; 15. Stop block; 16. Motor; 17. Rotating shaft; 18. First gear; 19. Slide plate; 20. Moving block; 21. Support ring; 22. Moving rod; 23. Reset spring; 24. Moving plate; 25. Abutment plate; 26. Support rod; 27. Telescopic rod. DETAILED DESCRIPTION

[0031] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions of the present invention will be clearly and completely described below in conjunction with the embodiments.

[0032] The present invention provides a device for detecting the survival rate and activity of microbial strains. Figure 1-3 As shown, it includes a device base plate 1, a temperature control mechanism 2 is slidably installed inside the device base plate 1, a snap-in groove is provided on the top surface of the device base plate 1, a heat conduction box 3 is snapped on the inner side of the snap-in groove, a culture medium plate 4 is provided inside the heat conduction box 3, and a mounting ring 5 is fixedly connected to the top of the device base plate 1 on the top outer side of the snap-in groove, a fixed block 6 is fixedly installed on one side of the heat conduction box 3 and on the top of the device base, a groove is provided on one side of the mounting block 7, a driving structure is provided inside the groove, a mounting block 7 is provided on one side of the driving structure, a connecting block 8 is fixedly installed in the middle position inside the mounting block 7, a clamping structure 9 is provided inside the connecting block 8, and a plug interface is provided on the top, a dropper 10 is provided inside the plug interface, a fixing plate 11 is provided at the bottom end of the dropper 10 and on one side of the fixed block 6, and a fixing device is provided inside the fixing plate 11.

[0033] The diluted bacterial liquid is poured into the dropper 10, and the bacterial liquid falls onto the culture medium plate 4 from the dropper 10. The temperature of the heat conduction box 3 is increased by the temperature control mechanism 2, and the hot air is transferred to the inside of the culture medium plate 4 through the heat conduction box 3, so that the water flow inside the culture medium plate 4 is dried. After reaching a certain dryness and humidity, the temperature control mechanism 2 is turned off. After completion, the driving structure is started to move the fixing plate 11 and the mounting block 7 upward, driving the dropper 10 to move upward, and at the same time, the clamping structure 9 no longer clamps the dropper 10. The bottom of the dropper 10 is supported by the fixing device inside the fixing plate 11 to prevent the dropper 10 from falling, making it easy to remove the dropper 10 and replace multiple droppers 10 at one time, thereby improving work efficiency.

[0034] like Figure 2 As shown, mounting grooves are provided on the front and rear sides of the mounting ring 5, a slot 12 is provided at the bottom of the mounting groove, an insert 13 is inserted into the inside of the slot 12, a compression spring 14 is fixedly connected to the top of the insert 13, a stopper 15 is fixedly connected to the bottom of the compression spring 14, and one side of the stopper 15 is fixedly connected to the outer surface of the heat conduction box 3.

[0035] Pulling the block 15 upward drives the heat conduction box 3 to move upward, so that the compression and stretching of the bottom of the block 15 drives the plug 13 to separate from the slot 12, and the heat conduction box 3 is removed from the equipment base plate 1, and the bacterial strains that have been left stationary in the culture medium plate 4 are separated, which is convenient for the next bacterial strain detection.

[0036] like Figure 2-3 As shown, the driving structure includes a motor 16, the bottom of the motor 16 is fixedly connected to the bottom inner wall of the groove, the output end of the motor 16 is fixedly connected to the rotating shaft 17, the top of the rotating shaft 17 is rotatably connected to the top inner wall of the groove, the top outer surface of the rotating shaft 17 is sleeved with a first gear 18, the bottom of the mounting block 7 is fixedly connected to a slide 19, a sliding groove is provided on one side of the groove, one side of the slide 19 is slidably connected to the sliding groove, the middle part of the slide 19 is fixedly sleeved with the outer surface of the rotating shaft 17, a through hole is provided in the middle part of the slide 19, and the rotating shaft 17 extends upward through the through hole.

[0037] The starting motor 16 drives the rotating shaft 17 and the first gear 18 to rotate. The rotation of the first gear 18 facilitates the clamping structure 9 to clamp and release the dropper 10. At the same time, the rotation of the rotating shaft 17 drives the slide 19 to move upward in the slide groove, so that the mounting block 7 drives the dropper 10 to move upward, driving the dropper 10 away from the culture medium plate 4, making it easier to replace the dropper 10.

[0038] like Figure 4-5As shown, the clamping structure 9 includes a second gear 91, a screw rod 92 is fixedly connected to the middle of the second gear 91, a first clamping plate 93 is fixedly sleeved on the outer surface of one end of the screw rod 92, and a second clamping plate 94 is fixedly sleeved on the outer surface of the other end thereof, the inner walls of the first clamping plate 93 and the second clamping plate 94 are fixedly connected to a buffer spring 95, one end of the buffer spring 95 is fixedly connected to a clamping block 96, the clamping block 96 is clamped with the dropper 10, the shape of the clamping block 96 is arc-shaped, and it is distributed in a central annular array of the connecting block 8, the clamping block 96 A rubber pad is provided inside, which fits the outer surface of the dropper 10. A movable opening is provided on the outer surfaces of both sides of the connecting block 8. The second gear 91 is located outside the mounting block 7. One end of the screw rod 92 passes through the first clamping block 96 and the second clamping block 96 and is rotatably connected to the inner wall of the mounting block 7. The second plywood 94 and the second plywood 94 are located inside the connecting block 8. The first plywood 93 and the second plywood 94 are both arc-shaped. Sockets are provided at both ends of the first plywood 93, and both ends of the second plywood 94 are plugged into the sockets.

[0039] The first clamping plate 93 and the second clamping plate 94 drive the clamping block 96 to clamp the dropper 10, and the rubber pad is used to increase the friction on the dropper 10 to fix the dropper 10 and prevent the dropper 10 from moving. The mounting block 7 is driven upward by the slide plate 19 to move the second gear 91 upward. When the first gear 18 moves to engage with the second gear 91, the second gear 91 rotates and drives the screw rod 92 to rotate, so that the first clamping block 96 and the second clamping block 96 move away from each other on the screw rod 92, so that the second clamping plate 94 is separated from the jack of the first clamping plate 93, so that the buffer spring 95 stretches and drives the clamping block 96 away from the dropper 10, so that the dropper 10 is no longer fixed.

[0040] like Figure 4 、 Figure 6 、 Figure 7 As shown, a moving block 20 is fixedly connected to one side of the fixed plate 11, one end of the moving block 20 is slidably connected to the slide groove, the middle part of the moving block 20 is fixedly sleeved with the rotating shaft 17, a fixing hole is opened at the top of the fixed plate 11, and a fixing groove is opened inside the fixed plate 11. The fixing device is located inside the fixing groove and on both sides of the dropper 10.

[0041] The motor 16 rotates to drive the rotating shaft 17 to rotate. Since the moving block 20 is fixedly connected to the rotating shaft 17, the moving plate 24 is driven to move upward when the rotating shaft 17 rotates, so that the fixed plate 11 and the dropper 10 can move upward and away from the culture medium plate 4 to facilitate the static placement of the bacterial strain. When the dropper 10 falls, the bottom of the dropper 10 is supported and fixed by the fixing device to prevent the dropper 10 from falling.

[0042] like Figure 6-7As shown, the fixing device includes a support ring 21, the top surface of the support ring 21 is fixedly connected to the top inner wall of the fixed groove, two movable grooves are opened on the top of the support ring 21, and the internal movability of the movable groove is connected with a movable rod 22, the bottom of the movable rod 22 is fixedly connected with a return spring 23, the bottom of the return spring 23 is fixedly connected with a movable plate 24, one end of the movable plate 24 is fixedly connected with a support plate 25, and the bottom of one end of the movable plate 24 is hinged with a support rod 26, the bottom of the support rod 26 is fixedly connected to the bottom inner wall of the fixed groove, and a telescopic rod 27 is fixedly connected to one side of the support rod 26 and located at the bottom of the movable plate 24.

[0043] The dropper 10 moves downward to squeeze the moving rod 22 on the top of the support ring 21, causing the moving rod 22 to move downward to squeeze the return spring 23. The moving plate 24 is subjected to the downward squeezing force and one end of the moving plate 24 deflects downward, while the other end drives the support plate 25 to deflect upward, causing the telescopic rod 27 to stretch. When the dropper 10 gradually falls and moves to the inside of the bottom fixing groove, the support plate 25 and the moving plate 24 support and fix the bottom of the dropper 10 to prevent the dropper 10 from moving downward and falling, making it easy to take out the dropper 10 for replacement.

[0044] like Figure 8 As shown, the present invention also provides a method for using the above-mentioned microbial strain survival rate activity detection device, which includes the following steps:

[0045] Step 1: First, the diluted bacterial solution is poured into the dropper 10. The bacterial solution falls from the dropper 10 onto the culture medium plate 4. The temperature control mechanism 2 increases the temperature of the heat conduction box 3. Hot air is transferred to the inner side of the culture medium plate 4 through the heat conduction box 3, drying the water flow inside the culture medium plate 4. After reaching a certain dryness and humidity, the temperature control mechanism 2 is turned off.

[0046] Step 2: Then start the motor 16, which drives the rotating shaft 17 and the first gear 18 to rotate, and at the same time drives the movable plate 24 and the slide plate 19 to move upward along the slide groove, so that the fixed plate 11 and the mounting block 7 move upward with the dropper 10, and the upward movement of the mounting block 7 drives the second gear 91 to move upward. When the second gear 91 moves and engages with the first gear 18, the first gear 18 rotates and drives the second gear 91 to rotate. The rotation of the second gear 91 drives the screw rod 92 to rotate, so that the first clamping plate 93 and the second clamping plate 94 move toward the two ends of the screw rod 92, driving the clamping block 96 away from the dropper 10, so that the dropper 10 is no longer clamped;

[0047] Step 3: Then the bottom end of the dropper 10 moves toward the inside of the fixed plate 11, so that the bottom end of the dropper 10 supports the moving rod 22 on the top of the extrusion ring, so that the moving rod 22 moves downward in the moving groove, and the return spring 23 is compressed, so that one end of the moving plate 24 deflects downward, and the other end drives the support plate 25 to deflect upward, so that the telescopic rod 27 extends and retracts. While the dropper 10 moves downward, the lower rod and the moving plate 24 support the bottom end of the dropper 10, and the dropper 10 is removed from the mounting block 7 and the fixed plate 11;

[0048] Step 4: After the final standing is completed, pull the block 15 upwards so that the block 15 drives the compression spring 14 to stretch, separating the plug 13 from the slot 12, thereby removing the culture medium plate 4 inside the thermal box 3 from the equipment base plate 1, replacing it with a new culture medium plate 4, and conducting the next test.

[0049] Working principle: When in use, the diluted bacterial liquid is poured into the dropper 10, and the bacterial liquid falls onto the culture medium plate 4 from the dropper 10. The temperature of the heat conduction box 3 is increased by the temperature control mechanism 2, and the hot air is transferred to the inside of the culture medium plate 4 through the heat conduction box 3, and the water flow inside the culture medium plate 4 is dried. After reaching a certain dryness and humidity, the temperature control mechanism 2 is turned off and the motor 16 is started. The motor 16 drives the rotating shaft 17 and the first gear 18 to rotate, and at the same time drives the movable plate 24 and the slide plate 19 to move upward along the slide groove, so that the fixed plate 11 and the mounting block 7 move upward with the dropper 10. The upward movement of the mounting block 7 drives the second gear 91 to move upward. When the second gear 91 moves and engages with the first gear 18, the first gear 18 rotates to drive the second gear 91 to rotate, and the rotation of the second gear 91 drives the screw rod 92 to rotate, so that the first clamping plate 93 and the second clamping plate 94 move toward the two ends of the screw rod 92, driving the clamping block 96 away from the dropper 10, so that the dropper 10 is no longer clamped and the dropper 10 falls.

[0050] The dropper 10 falls, causing the bottom end of the dropper 10 to move toward the inside of the fixed plate 11, and supporting the moving rod 22 on the top of the support ring 21 at the bottom end of the dropper 10, so that the moving rod 22 moves downward in the moving groove, and the return spring 23 is compressed, causing one end of the moving plate 24 to deflect downward, and the other end drives the support plate 25 to deflect upward, causing the telescopic rod 27 to extend and retract. While the dropper 10 moves downward, the low rod and the moving plate 24 support the bottom end of the dropper 10, and then the dropper 10 is removed from the mounting block 7 and the fixed plate 11. After standing still, pull the block 15 upward, so that the block 15 drives the compression spring 14 to stretch, and separates the insert 13 from the slot 12, thereby removing the culture medium plate 4 inside the thermal box 3 from the equipment base plate 1, replacing the new culture medium plate 4, and performing the next inspection.

[0051] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same.

Claims

1. A device for detecting the survival rate and activity of microbial strains, comprising: The device base plate (1) is provided with a temperature control mechanism (2) which is slidably installed inside the device base plate (1), a clamping groove is provided on the top surface of the device base plate (1), a heat conduction box (3) is clamped inside the clamping groove, and a culture medium plate (4) is provided inside the heat conduction box (3), and the top outer side of the clamping groove is fixedly connected to the top of the device base plate (1) with a mounting ring (5), a fixed block (6) is fixedly installed on one side of the heat conduction box (3) and located at the top of the device base, and the mounting block (7 ) is provided with a groove on one side, a driving structure is provided inside the groove, a mounting block (7) is provided on one side of the driving structure, a connecting block (8) is fixedly installed at a middle position inside the mounting block (7), a clamping structure (9) is provided inside the connecting block (8), and a plug interface is provided on the top thereof, a dropper (10) is provided inside the plug interface, a fixing plate (11) is provided at the bottom end of the dropper (10) and located on one side of the fixing block (6), and a fixing device is provided inside the fixing plate (11).

2. A microbial strain survival rate activity detection device according to claim 1, characterized in that: The front and rear sides of the mounting ring (5) are both provided with mounting grooves, the bottom of the mounting groove is provided with a slot (12), an insert (13) is inserted into the interior of the slot (12), the top of the insert (13) is fixedly connected to a compression spring (14), the bottom of the compression spring (14) is fixedly connected to a stopper (15), and one side of the stopper (15) is fixedly connected to the outer surface of the heat conduction box (3).

3. The microbial strain survival rate and activity detection device according to claim 1, characterized in that: The driving structure comprises a motor (16), the bottom of the motor (16) is fixedly connected to the bottom inner wall of the groove, the output end of the motor (16) is fixedly connected to a rotating shaft (17), the top of the rotating shaft (17) is rotatably connected to the top inner wall of the groove, and the outer surface of the top end of the rotating shaft (17) is fixedly sleeved with a first gear (18).

4. The microbial strain survival rate and activity detection device according to claim 3, characterized in that: The bottom of the mounting block (7) is fixedly connected to a slide plate (19), one side of the groove is provided with a slide groove, one side of the slide plate (19) is slidably connected to the slide groove, the middle part of the slide plate (19) is fixedly sleeved with the outer surface of the rotating shaft (17), the middle part of the slide plate (19) is provided with a through hole, and the rotating shaft (17) extends upward through the through hole.

5. The microbial strain survival rate and activity detection device according to claim 1, characterized in that: The clamping structure (9) includes a second gear (91), a screw rod (92) is fixedly connected to the middle part of the second gear (91), a first clamping plate (93) is fixedly sleeved on the outer surface of one end of the screw rod (92), and a second clamping plate (94) is fixedly sleeved on the outer surface of the other end thereof, the inner walls of the first clamping plate (93) and the second clamping plate (94) are fixedly connected to a buffer spring (95), and one end of the buffer spring (95) is fixedly connected to a clamping block (96).

6. The microbial strain survival rate and activity detection device according to claim 5, characterized in that: Both sides of the outer surface of the connecting block (8) are provided with movable openings, the second gear (91) is located outside the mounting block (7), one end of the screw rod (92) passes through the first clamping block (96) and the second clamping block (96) and is rotatably connected to the inner wall of the mounting block (7), the second clamping plate (94) and the second clamping plate (94) are located inside the connecting block (8), the first clamping plate (93) and the second clamping plate (94) are both arc-shaped, both ends of the first clamping plate (93) are provided with sockets, and both ends of the second clamping plate (94) are plugged into the sockets.

7. The microbial strain survival rate and activity detection device according to claim 5, characterized in that: The clamping block (96) is clamped with the dropper (10), the clamping block (96) is arc-shaped, and is distributed in a central annular array of the connecting block (8), and a rubber pad is provided inside the clamping block (96), and the rubber pad is in contact with the outer surface of the dropper (10).

8. The microbial strain survival rate and activity detection device according to claim 1, characterized in that: A moving block (20) is fixedly connected to one side of the fixed plate (11), one end of the moving block (20) is slidably connected to the slide groove, the middle part of the moving block (20) is fixedly sleeved with the rotating shaft (17), a fixing hole is provided on the top of the fixed plate (11), a fixing groove is provided inside the fixed plate (11), and the fixing device is located inside the fixing groove and on both sides of the dropper (10).

9. The microbial strain survival rate and activity detection device according to claim 8, characterized in that: The fixing device comprises a support ring (21), the top surface of the support ring (21) is fixedly connected to the top inner wall of the fixed groove, two movable grooves are provided on the top of the support ring (21), and the two movable grooves are movably connected to a movable rod (22) inside, the bottom of the movable rod (22) is fixedly connected to a reset spring (23), the bottom of the reset spring (23) is fixedly connected to a movable plate (24), one end of the movable plate (24) is fixedly connected to a push plate (25), the bottom of one end of the movable plate (24) is hinged to a support rod (26), the bottom of the support rod (26) is fixedly connected to the bottom inner wall of the fixed groove, and a telescopic rod (27) is fixedly connected to one side of the support rod (26) and located at the bottom of the movable plate (24).

10. A method for using the microbial strain survival rate activity detection device according to any one of claims 1 to 9, characterized in that: The method comprises the following steps: Step 1: First, the diluted bacterial solution is poured into the dropper (10), and the bacterial solution falls from the dropper (10) onto the culture medium plate (4). The temperature of the heat conduction box (3) is increased by the temperature control mechanism (2), and hot air is transferred to the inner side of the culture medium plate (4) through the heat conduction box (3), so that the water flow inside the culture medium plate (4) is dried. After reaching a certain dryness and wetness, the temperature control mechanism (2) is closed; Step 2: Then start the motor (16), the motor (16) drives the rotating shaft (17) and the first gear (18) to rotate, and at the same time drives the movable plate (24) and the slide plate (19) to move upward along the slide groove, so that the fixed plate (11) and the mounting block (7) move upward with the dropper (10), and the mounting block (7) moves upward to drive the second gear (91) to move upward. When the second gear (91) moves and engages with the first gear (18), the first gear (18) rotates to drive the second gear (91) to rotate, and the second gear (91) rotates to drive the screw rod (92) to rotate, so that the first clamping plate (93) and the second clamping plate (94) move toward the two ends of the screw rod (92), driving the clamping block (96) away from the dropper (10), so that the dropper (10) is no longer clamped; Step 3: Then the bottom end of the dropper (10) moves toward the inside of the fixed plate (11), so that the bottom end of the dropper (10) supports the moving rod (22) on the top of the extrusion ring, so that the moving rod (22) moves downward in the moving groove, and the return spring (23) is compressed, so that one end of the moving plate (24) deflects downward, and the other end drives the support plate (25) to deflect upward, so that the telescopic rod (27) is telescoped. When the dropper (10) moves downward, the lower rod and the moving plate (24) support the bottom end of the dropper (10), and then the dropper (10) is removed from the mounting block (7) and the fixed plate (11); Step 4: After the final resting is completed, pull the block (15) upwards so that the block (15) drives the compression spring (14) to stretch, separating the plug (13) from the slot (12), thereby removing the culture medium plate (4) inside the heat conduction box (3) from the equipment base plate (1), replacing it with a new culture medium plate (4), and performing the next test.

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