A temperature detection device and method applied to recovery in NK cell preparation process

Abstract

The application discloses a temperature detection device and method applied to recovery in an NK cell preparation process, relates to the technical field of NK cell preparation, and comprises a servo motor and a temperature detection assembly. The output end of the servo motor is connected with a shaking table. The temperature detection assembly is arranged on one side of a hole site. A probe is fixed to the bottom of a temperature sensor body. A sleeve plate is arranged on the lower part of the outer wall of the probe. A sleeve box is fixed to the upper part of the outer wall of the probe. A rotating ring is connected to the inner wall of the bearing. The temperature detection device and method applied to recovery in the NK cell preparation process can provide a water body with stable and persistent temperature for the continuous heat absorption process of the freezing tube. When the water body flows, the transmission blade is pushed to make the rotating ring rotate through the bearing, so that the second detection end can detect the temperature of different parts of the outer wall of the freezing tube. The second detection end can shake synchronously with the freezing tube and always keep stable temperature detection.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of NK cell preparation, in particular to a temperature detection device and method applied to the recovery in the NK cell preparation process. BACKGROUND

[0002] NK cells, also known as natural killer cells, are an important type of immune cells and belong to the part of the innate immune system. They play a role in recognizing and eliminating infected cells, tumor cells and some abnormal cells in the human body without prior antigen contact or specific immune response activation. NK cells usually involve several steps such as collection, enrichment, expansion, cryopreservation and recovery in the preparation process. When NK cells are converted from the cryopreservation state to the use state, they need to be thawed and recovered through the recovery step. Usually, the frozen reagent tube is placed in a 37-degree Celsius water bath and gently shaken to quickly thaw it. In the recovery process, the water bath temperature and the temperature of the frozen reagent tube need to be monitored in real time by a temperature detection device to strictly control the temperature in the recovery process.

[0003] In the existing recovery process of NK cells, the frozen reagent tube needs to be gently shaken. If the detection end of the temperature detection device is inside the reagent tube, the internal cells are easily damaged by shaking. If the detection end is outside the reagent tube, it is difficult to monitor the temperature of the reagent tube in real time according to the shaking state, resulting in discontinuous and inaccurate temperature detection.

[0004] Therefore, in view of the above problems, the present application provides a temperature detection device and method applied to the recovery in the NK cell preparation process. SUMMARY

[0005] In view of the deficiencies of the prior art, the present application provides a temperature detection device and method applied to the recovery in the NK cell preparation process, which solves the problems raised in the background art.

[0006] To achieve the above purpose, the present application realizes the following technical scheme: a temperature detection device applied to the recovery in the NK cell preparation process, comprising a servo motor and a temperature detection assembly, the output end of the servo motor is connected with a shaking table, and the surface of the shaking table is provided with equidistant hole positions, the temperature detection assembly is arranged on one side of the hole positions, the temperature detection assembly comprises a temperature sensor main body, a probe, a first detection end, a sleeve plate, a second detection end, a sleeve box, a bearing, a rotating ring and a rubber sleeve, the bottom of the temperature sensor main body is fixed with the probe, the bottom of the probe is provided with the first detection end, the outer wall of the probe is sleeved with the sleeve plate at the lower part, the side surface of the sleeve plate is provided with the second detection end, the outer wall of the probe is fixed with the sleeve box at the upper part, the inner wall of the sleeve box is provided with the bearing at the top and the bottom, the inner wall of the bearing is connected with the rotating ring, and the inner wall of the rotating ring is fixed with the rubber sleeve.

[0007] Further, the vertical central axis of the rotating ring coincides with the vertical central axis of the hole position, and a refrigeration tube is arranged in the hole position.

[0008] Further, the temperature detection assembly further comprises an annular groove, and the inner portion of the sleeve box is provided with the annular groove.

[0009] Further, the temperature detection assembly further comprises a water inlet end, and one side of the outer wall of the sleeve box is provided with the water inlet end.

[0010] Further, the temperature detection assembly further comprises a micro pump, and the end portion of the water inlet end is connected with the micro pump.

[0011] Further, the temperature detection assembly further comprises a water inlet pipe, and the end portion of the micro pump is connected with the water inlet pipe, and the end portion of the water inlet pipe is located at the side of the inner wall of the water bath tank.

[0012] Further, the temperature detection assembly further comprises a transmission blade, and the outer wall of the rotating ring is fixed with the transmission blade.

[0013] Further, the temperature detection assembly further comprises a water outlet end, and the outer wall of the sleeve box is provided with the water outlet end at the opposite side of the water inlet end.

[0014] Further, the temperature detection assembly further comprises a spiral water distribution pipe, and the end portion of the water outlet end is connected with the spiral water distribution pipe, and the outer wall of the spiral water distribution pipe is in a microporous structure.

[0015] A temperature detection method applied to the recovery in the preparation process of NK cells, which is applied to the temperature detection device for the recovery in the preparation process of NK cells, and the temperature detection method applied to the recovery in the preparation process of NK cells comprises the following steps.

[0016] Step one: before the recovery, the temperature sensor body collects the water temperature around the rotating ring by using the first detection end, and because the water bath tank is heated, the temperature of the side of the tank is higher than the temperature of the middle part, based on this, the water in the side area of the tank is extracted by the micro pump and the water inlet pipe, so that the relatively high-temperature water passes through the water inlet end, the annular groove and the water outlet end in turn and is discharged from the micropores on the surface of the spiral water distribution pipe, so that the relatively high-temperature water in the side area of the tank is transmitted to the periphery of the rotating ring to ensure the uniformity of the water temperature.

[0017] Step two: the first detection end synchronously detects with the temperature sensor at the inner side of the water bath tank, when the temperature of each area reaches the preset temperature, the frozen tube to be recovered is inserted into the hole position from top to bottom, rotates inside the ring, and the rubber sleeve is used to strengthen the clamping firmness of the frozen tube, the servo motor is bidirectionally and uniformly rotated to drive the shaking table to uniformly shake, the frozen tube shakes in the water bath tank, the outer wall of the frozen tube is closely attached to the arc surface of the sleeve plate, and the second detection end is used to monitor the temperature of the outer wall of the frozen tube in real time; and the second detection end synchronously shakes with the frozen tube;

[0018] Step three: during the recovery process of the frozen tube, the micro pump still keeps conveying the water in the tank to the periphery of the rotating ring to ensure that the water temperature outside the frozen tube is moderate and within the temperature threshold required for recovery, and when the water flows along the inner part of the annular groove, the transmission blade is pushed to make the rotating ring rotate through the bearing, so that the frozen tube rotates on the arc surface of the sleeve plate, and thus the second detection end can detect the temperature of different parts of the outer wall of the frozen tube.

[0019] The application provides a temperature detection device and method applied to recovery in an NK cell preparation process, and has the following beneficial effects:

[0020] 1. The temperature detection device and method applied to recovery in the NK cell preparation process, since the temperature sensor of the water bath tank is usually arranged at the side, the water temperature at the inner side of the tank is more accurate, the relatively accurate water in the tank is extracted by the micro pump at the inner side to reach the periphery of the frozen tube, the water with stable and lasting temperature is provided for the continuous heat absorption process of the frozen tube recovery, so that the frozen tube recovery conditions are up to standard and more accurate, and secondly, when the water flows along the inner part of the annular groove, the transmission blade is pushed to make the rotating ring rotate through the bearing, so that the frozen tube rotates on the arc surface of the sleeve plate, and thus the second detection end can detect the temperature of different parts of the outer wall of the frozen tube, and the second detection end can synchronously shake with the frozen tube and always keep stable temperature detection.

[0021] 2. The temperature detection device and method applied to recovery in the NK cell preparation process, before recovery, the temperature sensor body collects the water temperature at the periphery of the rotating ring by the first detection end, since when the water in the water bath tank is heated, the temperature at the inner side of the tank is higher than that in the middle, based on this, the water in the tank is extracted by the micro pump and the water inlet pipe, so that the relatively high-temperature water passes through the water inlet end, the annular groove and the water outlet end in turn and is discharged from the micropores on the surface of the spiral water distribution pipe, so that the relatively high-temperature water at the inner side of the tank is transmitted to the periphery of the rotating ring to ensure uniform water temperature, so as to shorten the heating time required for uniform water temperature, and the water bath tank reaches the preset temperature faster to perform water bath recovery work. BRIEF DESCRIPTION OF DRAWINGS

[0022] Figure 1 Structure diagram of the shaker when the freezing tube is clamped;

[0023] Figure 2 Structure diagram of the shaker when the freezing tube is not clamped;

[0024] Figure 3 Structure diagram of the inside of the box before the freezing tube is clamped;

[0025] Figure 4 Structure diagram of the inside of the box after the freezing tube is clamped;

[0026] Figure 5 Structure diagram of the inside of the box.

[0027] In the figure: 1, servo motor; 2, shaker; 3, hole site; 4, temperature detection assembly; 401, temperature sensor main body; 402, probe; 403, first detection end; 404, sleeve plate; 405, second detection end; 406, box; 407, bearing; 408, rotating ring; 409, rubber sleeve; 410, annular groove; 411, water inlet end; 412, micro pump; 413, water inlet pipe; 414, transmission blade; 415, water outlet end; 416, spiral water distribution pipe; 5, freezing tube. DETAILED DESCRIPTION

[0028] The embodiments of the present application will be further described below in conjunction with the drawings and examples. The following examples are used to illustrate the present application, but cannot be used to limit the scope of the present application.

[0029] As Figures 1-5As shown, the present application provides technical solutions: a temperature detection device applied to the recovery of NK cell preparation process, comprising a servo motor 1 and a temperature detection assembly 4, the output end of the servo motor 1 is connected with a rocking bed 2, and the surface of the rocking bed 2 is equidistantly provided with a hole position 3, the temperature detection assembly 4 is arranged on one side of the hole position 3, the temperature detection assembly 4 comprises a temperature sensor main body 401, a probe 402, a first detection end 403, a sleeve plate 404, a second detection end 405, a sleeve box 406, a bearing 407, a rotating ring 408 and a rubber sleeve 409, the bottom of the temperature sensor main body 401 is fixedly provided with the probe 402, the bottom of the probe 402 is provided with the first detection end 403, the outer wall of the probe 402 is sleeved with the sleeve plate 404, the side of the sleeve plate 404 is provided with the second detection end 405, the outer wall of the probe 402 is fixedly provided with the sleeve box 406, the inner wall of the sleeve box 406 is provided with the bearing 407, the inner wall of the bearing 407 is connected with the rotating ring 408, the inner wall of the rotating ring 408 is fixedly provided with the rubber sleeve 409, the vertical central axis of the rotating ring 408 coincides with the vertical central axis of the hole position 3, and the inside of the hole position 3 is provided with a freezing tube 5, the temperature detection assembly 4 further comprises an annular groove 410, the inside of the sleeve box 406 is provided with the annular groove 410, the temperature detection assembly 4 further comprises a water inlet end 411, one side of the outer wall of the sleeve box 406 is provided with the water inlet end 411, the temperature detection assembly 4 further comprises a micro pump 412, the end of the water inlet end 411 is connected with the micro pump 412, the temperature detection assembly 4 further comprises a water inlet pipe 413, the end of the micro pump 412 is connected with the water inlet pipe 413, and the end of the water inlet pipe 413 is located at the side of the inner wall of the water bath tank, the temperature detection assembly 4 further comprises a transmission blade 414, the outer wall of the rotating ring 408 is fixedly provided with the transmission blade 414, the temperature detection assembly 4 further comprises a water outlet end 415, the outer wall of the sleeve box 406 is provided with the water outlet end 415 on the opposite side of the water inlet end 411, the temperature detection assembly 4 further comprises a spiral water distribution pipe 416, the end of the water outlet end 415 is connected with the spiral water distribution pipe 416, and the outer wall of the spiral water distribution pipe 416 is in a microporous structure;

[0030] The specific operation is as follows: since the heating assembly of the water bath tank is closer to the side of the water body, the heating direction of the water body is from the outside to the inside, which causes the temperature of the outer water body to be higher than the temperature of the middle of the water body in the heating state, so as to prolong the heating time of the water body to maintain the overall temperature of the water body, thereby ensuring the uniform temperature of the water body;

[0031] And the temperature sensor body 401 collects the water temperature around the rotating ring 408 by using the first detection end 403 before the resuscitation, and because the water bath tank is heated when the water inside the tank, the temperature is usually higher on the side than in the middle, based on this, the water in the side area of the tank is extracted by the micro pump 412 and the water inlet pipe 413, so that the relatively high temperature water passes through the water inlet end 411, the annular groove 410 and the water outlet end 415 in turn and is discharged from the micro holes on the surface of the spiral water pipe 416, so that the relatively high temperature water in the side area of the tank is transmitted to the periphery of the rotating ring 408 to ensure uniform water temperature, thereby shortening the heating time required for uniform water temperature, so that the water bath tank reaches the preset temperature faster to carry out the water bath resuscitation operation;

[0032] The first detection end 403 and the temperature sensor in the side area of the water bath tank are detected synchronously, when the temperature of each area reaches the preset temperature, the frozen pipe 5 to be resuscitated is inserted into the hole position 3 inside the rotating ring 408 from top to bottom in turn, and the rubber sleeve 409 is used to strengthen the clamping firmness of the frozen pipe 5, the servo motor 1 is bidirectional and uniform speed to drive the shaking table 2 to shake uniformly, the frozen pipe 5 shakes with the shaking table 2 in the water bath tank to resuscitate, at this time, the outer wall of the frozen pipe 5 is closely attached to the arc surface of the sleeve plate 404, and the second detection end 405 is used to monitor the temperature of the outer wall of the frozen pipe 5 in real time, and the second detection end 405 shakes synchronously with the frozen pipe 5, and in the process of resuscitation of the frozen pipe 5, the micro pump 412 still keeps the water in the side area of the tank to the periphery of the rotating ring 408 to ensure that the water temperature outside the frozen pipe 5 is moderate and within the temperature threshold required for resuscitation, at the same time, when the water flows along the inside of the annular groove 410, it pushes the transmission blade 414 to make the rotating ring 408 rotate through the bearing 407, so that the frozen pipe 5 is carried on the arc surface of the sleeve plate 404, so that the second detection end 405 can detect the temperature of different parts of the outer wall of the frozen pipe 5;

[0033] Based on the above description, because the temperature sensor of the water bath tank is usually arranged at the side, the water temperature in the side area of the tank is more accurate, the relatively accurate water in the side area of the tank is extracted by using the micro pump 412 to reach the periphery of the frozen pipe 5, which provides stable and lasting water for the resuscitation of the frozen pipe 5 during the continuous heat absorption process, so as to ensure that the resuscitation condition of the frozen pipe 5 meets the standard and is more accurate, secondly, when the water flows along the inside of the annular groove 410, it pushes the transmission blade 414 to make the rotating ring 408 rotate through the bearing 407, so that the frozen pipe 5 is carried on the arc surface of the sleeve plate 404, so that the second detection end 405 can detect the temperature of different parts of the outer wall of the frozen pipe 5, and the second detection end 405 can shake synchronously with the frozen pipe 5 and always keep stable temperature detection.

[0034] As Figures 1-5As shown, a temperature detection method applied to the recovery in the NK cell preparation process, which applies the temperature detection device applied to the recovery in the NK cell preparation process, the temperature detection method applied to the recovery in the NK cell preparation process comprises the following steps:

[0035] Step one: before recovery, the temperature sensor body 401 uses the first detection end 403 to collect the water temperature around the rotating ring 408, and because the water bath tank is heated inside the water, the temperature is often higher than the middle temperature, based on this, the water in the side area of the tank is extracted through the micro pump 412 and the water inlet pipe 413, so that the relatively high temperature water passes through the water inlet end 411, the annular groove 410 and the water outlet end 415 in turn and is discharged from the micropore on the surface of the spiral water pipe 416, so that the relatively high temperature water in the side area of the tank is transmitted to the periphery of the rotating ring 408 to ensure uniform water temperature;

[0036] Step two: the first detection end 403 and the temperature sensor in the side area of the water bath tank are synchronously detected, when the temperature of each area reaches the preset temperature, the frozen tube 5 to be recovered is inserted into the hole position 3 and the inside of the rotating ring 408 from top to bottom, and the rubber sleeve 409 is used to enhance the clamping firmness of the frozen tube 5, the servo motor 1 is bidirectionally rotated at a constant speed to drive the shaking table 2 to rotate at a constant speed, and the frozen tube 5 is recovered in the water bath tank with the shaking table 2, at this time, the outer wall of the frozen tube 5 is tightly attached to the arc surface of the sleeve plate 404, and the second detection end 405 is used to monitor the temperature of the outer wall of the frozen tube 5 in real time, and the second detection end 405 is synchronously shaken with the frozen tube 5;

[0037] Step three: in the process of recovering the frozen tube 5, the micro pump 412 still transports the water in the side area of the tank to the periphery of the rotating ring 408 to ensure that the water temperature outside the frozen tube 5 is moderate and within the temperature threshold required for recovery, and when the water flows along the inside of the annular groove 410, it pushes the transmission blade 414 to make the rotating ring 408 rotate through the bearing 407, so that the frozen tube 5 is attached to the arc surface of the sleeve plate 404, thereby enabling the second detection end 405 to detect the temperature of different parts of the outer wall of the frozen tube 5.

[0038] Embodiments of the present application are given for the purpose of illustration and description, and are not intended to be exhaustive or to limit the application to the disclosed form. Many modifications and variations will be apparent to those of ordinary skill in the art. Embodiments are chosen and described in order to best explain the principles of the application and its practical application, and to enable others skilled in the art to understand the application for various embodiments with various modifications as are suited to the particular use contemplated.

Claims

1. A temperature detection device for resuscitation during NK cell preparation, comprising a servo motor (1) and a temperature detection component (4), characterized in that: The output end of the servo motor (1) is connected to a shaking table (2), and holes (3) are equidistantly opened on the surface of the shaking table (2). The temperature detection component (4) is disposed on one side of the holes (3). The temperature detection component (4) includes a temperature sensor body (401), a probe (402), a first detection end (403), a sleeve (404), a second detection end (405), a housing (406), a bearing (407), a rotating ring (408), and a rubber sleeve (409). The probe (402) is fixed at the bottom of the temperature sensor body (401), and the bottom of the probe (402) is provided with The first detection end (403) is fitted with a sleeve plate (404) on the lower part of the outer wall of the probe (402), and a second detection end (405) is provided on the side of the sleeve plate (404). A sleeve box (406) is fixed on the upper part of the outer wall of the probe (402), and bearings (407) are provided at the top and bottom of the inner wall of the sleeve box (406). A rotating ring (408) is connected to the inner wall of the bearing (407), and a rubber sleeve (409) is fixed to the inner wall of the rotating ring (408). The vertical central axis of the rotating ring (408) coincides with the vertical central axis of the hole (3), and a hole (3) is inserted inside. The temperature detection component (4) includes a freezing tube (5), an annular groove (410) is provided inside the housing (406), the temperature detection component (4) also includes a water inlet (411) is provided on one side of the outer wall of the housing (406), the temperature detection component (4) also includes a micro pump (412) is connected to the end of the water inlet (411), the temperature detection component (4) also includes a water inlet pipe (413) is connected to the end of the micro pump (412), and the water inlet pipe... The end of (413) is located on the side of the inner wall of the water bath. The temperature detection component (4) also includes a transmission blade (414). The transmission blade (414) is fixed in the middle of the outer wall of the rotating ring (408). The temperature detection component (4) also includes a water outlet (415). The outer wall of the sleeve (406) is provided with a water outlet (415) on the opposite side of the water inlet (411). The temperature detection component (4) also includes a spiral water distribution pipe (416). The end of the water outlet (415) is connected to the spiral water distribution pipe (416), and the outer wall of the spiral water distribution pipe (416) has a microporous structure.

2. A method for detecting the temperature of resuscitation during NK cell preparation, comprising the temperature detection device for resuscitation during NK cell preparation as described in claim 1, characterized in that: The temperature detection method applied to the resuscitation process of NK cells includes the following steps: Step 1: Before the recovery, the temperature sensor body (401) collects the water temperature around the rotating ring (408) using the first detection end (403). Since the temperature of the water bath is often higher at the side of the tank than in the middle when the water bath is heating the water inside, the water in the side area of ​​the tank is extracted by the micro pump (412) and the inlet pipe (413). The relatively high temperature water passes through the inlet end (411), the annular tank (410), and the outlet end (415) in sequence and is discharged from the micropores on the surface of the spiral water distribution pipe (416). This allows the relatively high temperature water in the side of the tank to be transferred to the area around the rotating ring (408) to ensure uniform water temperature. Step 2: The first detection end (403) and the temperature sensor on the side of the water bath are detected synchronously. When the temperature of each area reaches the preset temperature, the cryotube (5) to be revived is inserted into the hole (3) and the rotating ring (408) from top to bottom. The rubber sleeve (409) is used to strengthen the clamping firmness of the cryotube (5). The servo motor (1) rotates bidirectionally at a constant speed to drive the shaker (2) to shake at a constant speed. The cryotube (5) is located in the water bath for revival along with the shaker (2). At this time, the outer wall of the cryotube (5) is tightly attached to the arc surface of the sleeve plate (404). The second detection end (405) is used to monitor the temperature of the outer wall of the cryotube (5) in real time. The second detection end (405) shakes synchronously with the cryotube (5). Step 3: During the revival process of the cryotube (5), the micro pump (412) continues to deliver water from the side of the tank to the periphery of the rotating ring (408) to ensure that the temperature of the water outside the cryotube (5) is moderate and within the temperature threshold required for revival. At the same time, as the water flows along the inside of the annular tank (410), it will push the transmission blades (414) to make the rotating ring (408) rotate through the bearing (407), thereby rotating the cryotube (5) against the arc surface of the sleeve plate (404), so that the second detection end (405) can detect the temperature of different parts of the outer wall of the cryotube (5).

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