Device and method for preparing scutellaria baicalensis compound fermentation liquor

Abstract

The invention belongs to the technical field of cosmetics, and discloses a preparation device of scutellaria baicalensis compound fermentation broth, which comprises a fermentation tank, the fermentation tank is provided with supporting legs, a console, a discharge port, a liquid adding pipe, a heating pipe, a bracket, a weighing device, a charging cylinder, an electromagnetic valve, a stirring mechanism, a closing mechanism, a feeding mechanism and a grinding mechanism; the preparation method comprises the following preparation steps: S1, removing impurities from baical skullcap root, lightyellow sophora root, liquoric root, purslane herb and Chinese gentian root, and putting into a grinding mechanism; s2, starting a grinding mechanism and a feeding mechanism, and feeding the raw materials into a fermentation tank in proportion by matching with a weighing device; s3, water is introduced into the fermentation tank through a liquid adding pipe for soaking; s4, performing enzymolysis; s5, obtaining a fermentation substrate; s6, anaerobic fermentation; s7, polyhydric alcohol is added; according to the radix scutellariae compound fermentation liquid, the modern traditional Chinese medicine theory is combined, a more effective traditional Chinese medicine formula aiming at improving the skin problems of people with sensitive skin is screened and designed, and the formula design is more reasonable.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics, and particularly relates to a device and method for preparing a scutellaria baicalensis composite fermentation liquid. Background Art

[0002] In recent years, due to ecological degradation and the overuse of various industrial additives, the number of people suffering from allergies has steadily increased, and skin allergies have become a major factor affecting human health. According to statistics from five dermatology diagnostic institutions, contact dermatitis accounts for over 80% of the total incidence of cosmetic-related skin diseases in recent years. Analysis of data from my country's Cosmetics Adverse Reaction Monitoring Network in recent years shows that contact dermatitis caused by cosmetic allergies or irritation accounts for over 90% of cosmetic skin diseases. Sensitive skin is a special skin type that, when exposed to external stimuli, is prone to objective symptoms such as erythema, papules, and dilated capillaries, accompanied by subjective symptoms such as itching, stinging, burning, and tightness.

[0003] Although relying on some hormone drugs can treat the disease more quickly on the surface, it will not cause recurrence in a short period of time. However, it will lead to severe recurrence over a long period of time, causing repeated attacks of allergic dermatitis and causing severe pain to patients.

[0004] "Purely natural, additive-free, and irritation-free" is the goal pursued by major cosmetics companies. Developing active ingredients from natural plants with anti-allergic, anti-itching, and restorative properties, and incorporating them into everyday skincare products to create natural anti-allergic cosmetics, has become a key research focus in the cosmetics industry. Furthermore, natural plant-derived anti-allergic active ingredients have minimal toxicity and side effects, generally do not cause drug dependency, and are easily accepted and recognized by patients. Therefore, research into natural plant-derived substances with anti-allergic, anti-inflammatory, and anti-itching properties, as well as exploring their pathways of action, is gaining increasing attention among scholars and researchers both domestically and internationally.

[0005] Fermentation refers to the theoretical and engineering system that utilizes the growth, reproduction, and metabolic activities of microorganisms through modern engineering techniques to industrially produce desired products. It is a crucial component of bioengineering and biotechnology, and is widely used in the food, biology, and cosmetics industries. Numerous reports have been published on the synthesis and extraction of bioactive substances using fermentation techniques. However, the effectiveness and effects of synthesis and extraction vary significantly depending on the fermentation substrate and process conditions. Therefore, further exploration of more fermentation substrate combinations and fermentation methods is needed to produce a wider range of products with improved efficacy to meet consumer demand.

[0006] Currently, a wide variety of skincare products claim to have anti-allergic and soothing properties. Among them, ingredients like dipotassium glycyrrhizate and bisabolol are the most common. While these ingredients offer promising results, they also present challenges such as high price, poor water solubility, and limited efficacy. Therefore, leveraging traditional medicine and combining its complex formulas with modern bio-fermentation technology to develop cosmetics that can improve sensitive skin and offer enhanced anti-allergic and soothing properties holds greater potential for application and commercial success. Summary of the Invention

[0007] In view of the problems raised by the above background technology, the purpose of the present invention is to provide a device and method for preparing a composite fermentation broth of Scutellaria baicalensis.

[0008] In order to achieve the above technical objectives, the technical solutions adopted by the present invention are as follows:

[0009] A preparation device for a scutellaria baicalensis composite fermentation liquid comprises a fermentation tank, the fermentation tank is equipped with supporting feet, a control console, a discharge port, a liquid adding pipe and a heating pipe, the fermentation tank is equipped with a liquid level gauge and a thermometer, the fermentation tank is equipped with a stirring mechanism, the top of the fermentation tank is provided with a plurality of feeding ports in a ring shape, the fermentation tank is equipped with a closing mechanism whose position corresponds to the feeding ports, the top of the fermentation tank is equipped with brackets whose position and number correspond to the feeding ports, a weighing device is fixedly mounted on the bracket, a feeding cylinder is placed at the center of the weighing device, the output end of the feeding cylinder is located at the feeding port, the feeding cylinder is equipped with a solenoid valve, a feeding mechanism is installed at the input end of the feeding cylinder, a grinding mechanism is installed at the input end of the feeding mechanism, the feeding mechanism is matched with a supporting frame, the supporting frame is equipped with a supporting seat, and the grinding mechanism is installed on the supporting seat;

[0010] A method for preparing a scutellaria baicalensis composite fermentation broth comprises the following steps:

[0011] S1. After removing impurities, the medicinal materials of Scutellaria baicalensis, Sophora flavescens, Glycyrrhiza uralensis, Portulaca oleracea, and Gentiana scabra are put into the grinding mechanism;

[0012] S2, starting the grinding mechanism and the feeding mechanism, and cooperating with the weighing device to feed the raw materials into the fermentation tank in proportion, wherein the mixing ratio of scutellaria baicalensis, sophora flavescens, liquorice, purslane and gentian is 3-5:1-3:1-2:2-3:1-3;

[0013] S3, adding 40-60°C water to the fermentation tank through the liquid adding pipe to soak for 1-4 hours, with a soaking solid-liquid ratio of 1:8-1:15;

[0014] S4, adding a complex enzyme preparation for enzymatic hydrolysis;

[0015] S5. Heating the liquid to 85-95° C., sterilizing and inactivating the enzyme for 20-30 min to obtain a fermentation substrate;

[0016] S6, inoculating compound lactobacillus freeze-dried powder and performing anaerobic fermentation;

[0017] S7, using a 100 mesh bag filter to separate and obtain a coarse filtrate, adding 8.0 to 15.0 wt% of polyol and stirring to mix evenly, and letting it stand for 2 to 4 hours after the mixing is completed;

[0018] S8. After the standing period, the mixed solution is filtered through a microporous cross-flow membrane, and the filtrate is packaged and sterilized to finally obtain the finished product of the Scutellaria baicalensis composite fermentation liquid.

[0019] It is further defined that the stirring mechanism is installed in a manner such that a working end thereof is eccentric to a central axis of the fermentation tank.

[0020] It is further defined that the closing mechanism includes a mounting shaft, a fork plate is installed on the mounting shaft, a gear ring is placed between the fork plate and the fermentation tank, a number of baffles corresponding to the feed port are fixedly installed on the outside of the gear ring, the outer edge of the baffle contacts the inner wall of the fermentation tank, the gear ring is meshed with a circular gear, the fermentation tank is installed with a servo motor, and the output end of the servo motor is connected to the central axis of the circular gear.

[0021] It is further defined that the grinding mechanism includes a grinding barrel, the bottom of the grinding barrel is in an inverted cone shape, the bottom of the grinding barrel is provided with grinding teeth, the area of the grinding barrel where the grinding teeth are provided is matched with a grinding table, the power end of the grinding table is connected to a grinding motor, and the grinding barrel is provided with a feed hopper.

[0022] It is further defined that in step S4, the complex enzyme preparation used for enzymatic hydrolysis is a complex enzyme preparation composed of cellulase, medium-temperature amylase, and papain, the ratio of which is 2-5:1-2:1-3, the enzyme dosage is 1.0-3.0 wt%, the enzymatic hydrolysis temperature is 45-55°C, and the enzymatic hydrolysis time is 1-4 hours.

[0023] Further defined, in step S6, the composite lactobacillus freeze-dried powder is prepared by mixing freeze-dried powder of Lactobacillus plantarum, Lactobacillus bulgaricus, Lactobacillus casei, and Bifidobacterium infantis in a ratio of 4:1:2:3 or 3:3:2:2 or 3:1:3:3 or 4:2:1:3 or 2:3:4:1, and the freeze-dried powder concentration of the strains is 10 9 -10 10 CFU / g.

[0024] It is further defined that in step S6, the anaerobic fermentation conditions are: inoculation amount of 0.5-1.0 wt%, fermentation temperature of 28° C.-42° C., liquid volume of 60.0-85.0 wt%, and fermentation time of 12-36 h.

[0025] It is further defined that in step S7, the polyol is butanediol.

[0026] It is further defined that in step S8, the sterilization temperature of the finished filtrate is 65-75°C and the sterilization time is 30-60 minutes.

[0027] The beneficial effects of the present invention are as follows:

[0028] 1. Combining modern Chinese medicine theory, we have screened and designed a more effective Chinese medicine formula to improve skin problems of people with sensitive skin. The formula design is more reasonable, applicable to a wider range of people, and safer.

[0029] 2. In the process flow, the medicinal materials are subjected to compound enzyme hydrolysis treatment: on the one hand, the anti-allergic and soothing components in the medicinal materials can be fully released, which can reduce the use of production raw materials to a certain extent, reduce production costs, and improve the actual efficacy of the product; on the other hand, through the catalytic hydrolysis of the compound enzyme, the nutrients in the medicinal materials that can be used as microbial fermentation can be converted and released into the fermentation substrate. Therefore, there is no need to add additional fermentation auxiliary raw materials to the fermentation substrate, which can reduce production costs and improve the stability of the fermentation process and fermentation products.

[0030] 3. The fermentation bacteria used are a carefully selected combination of Lactobacillus species. Through biotransformation during the fermentation process, the Lactobacillus species not only fully release the active ingredients in the fermentation substrate, but also enhance their bioactivity and stability. Furthermore, during the fermentation process, the Lactobacillus species also synthesize a large number of beneficial bioactive substances unique to Lactobacillus species, including organic acids, exopolysaccharides, and lactobacilli. These substances maintain the skin's microecological balance, improve the skin's microbial composition, strengthen the microbial protective film, and promote skin regeneration.

[0031] 4. The Scutellaria baicalensis composite fermentation broth obtained through the present invention can be used as an auxiliary material or functional ingredient in cosmetics to prepare cosmetics with anti-allergic and repairing effects, such as creams, lotions, essences, facial masks, and toners. The Scutellaria baicalensis composite fermentation broth provided by the present invention can effectively relieve allergic symptoms such as redness, dryness, itching, burning, and stinging. It can also promote keratinocyte proliferation and differentiation, accelerate epidermal regeneration, repair the skin barrier, and reshape the skin's biochemical function. Adding it to cosmetics as a functional ingredient can achieve excellent anti-allergic and repairing effects.

[0032] 5. Fermentation conditions such as the solid-liquid ratio, fermentation temperature, inoculum size, and liquid volume are optimized, and the optimal fermentation process is determined based on indicators such as the pH value and soluble solids content of the fermentation liquid. Therefore, the fermentation process of the present invention is simple to control and easy to operate, has relatively low requirements for product production line equipment, is easy to implement large-scale industrial production, can be widely promoted, and has a good market prospect.

[0033] 6. The present invention realizes the automatic grinding of raw materials and can automatically carry out quantitative proportioning of multiple raw materials. It is highly automatic and adaptable to the rapidly developing society. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] The present invention can be further illustrated by the non-limiting examples given in the accompanying drawings;

[0035] Figure 1 This is a schematic structural diagram of an embodiment of a device for preparing a composite fermentation liquid of Scutellaria baicalensis according to the present invention;

[0036] Figure 2 Schematic diagram of the cross-sectional structure of an embodiment of a device for preparing a composite fermentation liquid of Scutellaria baicalensis according to the present invention;

[0037] Figure 3 This is a partial exploded structural diagram of an embodiment of a device for preparing a composite fermentation liquid of Scutellaria baicalensis according to the present invention;

[0038] Figure 4 It is a partial structural schematic diagram of an embodiment of a device for preparing a composite fermentation liquid of Scutellaria baicalensis according to the present invention;

[0039] Figure 5 This is a schematic flow chart of an embodiment of a method for preparing a composite fermentation broth of Scutellaria baicalensis according to the present invention;

[0040] Figure 6 The line graph shows that the Scutellaria baicalensis compound water extract and the Scutellaria baicalensis compound fermentation liquid were prepared into solutions of different concentrations ranging from 2% to 20% and used as sample solutions for the in vitro hyaluronidase inhibition rate test;

[0041] Figure 7 This is a picture of the initial allergic reaction on the volunteer's arm;

[0042] Figure 8 This is a comparison picture of volunteers 2 hours after applying cream with 10% Scutellaria baicalensis compound fermentation liquid (the right half is the place where the cream was applied).

[0043] The main component symbols are described as follows:

[0044] Fermentation tank 1; support leg 2; control console 3; discharge port 4; liquid adding pipe 5; heating pipe 6; liquid level gauge 7; thermometer 8; stirring mechanism 9; feeding port 10; closing mechanism 11; bracket 12; weighing device 13; feeding cylinder 14; solenoid valve 15; feeding mechanism 16; grinding mechanism 17; support frame 18; support base 19;

[0045] Mounting shaft 111; fork plate 112; gear ring 113; baffle 114; circular gear 115; servo motor 116;

[0046] Grinding barrel 171; grinding table 172; grinding motor 173; feeding hopper 174. DETAILED DESCRIPTION

[0047] In order to enable those skilled in the art to better understand the present invention, the technical solution of the present invention is further described below with reference to the accompanying drawings and embodiments.

[0048] like Figures 1 to 4 As shown, a preparation device for a scutellaria baicalensis composite fermentation liquid of the present invention comprises a fermentation tank 1, the fermentation tank 1 is equipped with a supporting foot 2, a control console 3, a discharge port 4, a liquid adding pipe 5 and a heating pipe 6, the fermentation tank 1 is configured and equipped with a liquid level meter 7 and a thermometer 8, the fermentation tank 1 is equipped with a stirring mechanism 9, a plurality of feeding ports 10 are provided in a ring shape on the top of the fermentation tank 1, the fermentation tank 1 is equipped with a closing mechanism 11 whose position corresponds to the feeding port 10, a bracket 12 whose position and number correspond to the feeding port 10 is installed on the top of the fermentation tank 1, a weighing device 13 is fixedly installed on the bracket 12, a feeding cylinder 14 is placed at the center of the weighing device 13, the output end of the feeding cylinder 14 is located at the feeding port 10, the feeding cylinder 14 is equipped with a solenoid valve 15, a feeding mechanism 16 is installed at the input end of the feeding cylinder 14, a grinding mechanism 17 is installed at the input end of the feeding mechanism 16, the feeding mechanism 16 is matched with a supporting frame 18, the supporting frame 18 is equipped with a supporting seat 19, and the grinding mechanism 17 is installed on the supporting seat 19;

[0049] A method for preparing a scutellaria baicalensis composite fermentation broth comprises the following steps:

[0050] S1, after removing impurities from the medicinal materials Scutellaria baicalensis, Sophora flavescens, Glycyrrhiza uralensis, Portulaca oleracea, and Gentiana scabra, put them into the grinding mechanism 17;

[0051] S2, start the grinding mechanism 17 and the feeding mechanism 16, cooperate with the weighing device 13 to feed the raw materials into the fermentation tank 1 in proportion, and the mixing ratio of scutellaria baicalensis, sophora flavescens, liquorice, purslane and gentian is 3-5:1-3:1-2:2-3:1-3;

[0052] S3, adding 40-60°C water to the fermentation tank 1 through the liquid adding pipe 5 to soak for 1-4 hours, with a soaking solid-liquid ratio of 1:8-1:15;

[0053] S4, adding a complex enzyme preparation for enzymatic hydrolysis;

[0054] S5. Heating the liquid to 85-95° C., sterilizing and inactivating the enzyme for 20-30 min to obtain a fermentation substrate;

[0055] S6, inoculating compound lactobacillus freeze-dried powder and performing anaerobic fermentation;

[0056] S7, using a 100 mesh bag filter to separate and obtain a coarse filtrate, adding 8.0 to 15.0 wt% of polyol and stirring to mix evenly, and letting it stand for 2 to 4 hours after the mixing is completed;

[0057] S8. After the standing period, the mixed solution is filtered through a microporous cross-flow membrane, and the filtrate is packaged and sterilized to finally obtain the finished product of the Scutellaria baicalensis composite fermentation liquid.

[0058] Preferably, the stirring mechanism 9 is installed with the working end eccentric to the central axis of the fermentation tank 1. Such a design can prevent the stirring liquid of the stirring mechanism 9 from forming a vortex at the center position inside the fermentation tank 1, that is, breaking the symmetrical flow pattern, generating non-uniform turbulence, reducing the existence of "dead zones" in the tank, and making the fluid form a more complex circulation path in the tank body, which is beneficial to the mixing and stirring effect. At the same time, it can effectively avoid the position of the fork plate 112 to prevent interference. In fact, the installation position of the stirring mechanism 9 can also be considered according to specific circumstances.

[0059] The preferred closing mechanism 11 includes a mounting shaft 111, on which a fork plate 112 is mounted, and a gear ring 113 is placed between the fork plate 112 and the fermentation tank 1, and baffles 114 corresponding to the number of the feed port 10 are fixedly mounted on the outside of the gear ring 113, and the outer edge of the baffle 114 contacts the inner wall of the fermentation tank 1, and the gear ring 113 is meshed with a circular gear 115. The fermentation tank 1 is equipped with a servo motor 116, and the output end of the servo motor 116 is connected to the central axis of the circular gear 115. With this design, the forward and reverse rotation of the servo motor 116 can drive the gear ring 113 to rotate forward and reverse, thereby realizing the distance and approach of the baffle 114 relative to the discharge port 4, achieving the purpose of opening and closing, and providing the fermentation tank 1 with a good fermentation environment. In fact, the closing and opening measures for the discharge port 4 can also be considered according to specific circumstances.

[0060] Preferably, the grinding mechanism 17 includes a grinding barrel 171, the bottom of the grinding barrel 171 is in an inverted cone shape, and the bottom of the grinding barrel 171 is provided with grinding teeth. The area of the grinding barrel 171 provided with the grinding teeth is matched with a grinding table 172, and the power end of the grinding table 172 is connected to a grinding motor 173, and the grinding barrel 171 is provided with a hopper 174. With this design, the grinding of medicinal materials can be achieved by controlling the operation of the grinding motor 173, which is simple, convenient and fast. In fact, the structure and selection of the grinding mechanism 17 can also be considered according to specific circumstances.

[0061] Preferably, in step S4, the complex enzyme preparation used for enzymatic hydrolysis is a complex enzyme preparation composed of cellulase, medium-temperature amylase, and papain, with a ratio of 2-5:1-2:1-3, an enzyme dosage of 1.0-3.0 wt%, an enzymatic hydrolysis temperature of 45-55° C., and an enzymatic hydrolysis time of 1-4 hours.

[0062] Preferably, in step S6, the composite lactobacillus freeze-dried powder is prepared by mixing freeze-dried powders of Lactobacillus plantarum, Lactobacillus bulgaricus, Lactobacillus casei and Bifidobacterium infantis in a ratio of 4:1:2:3 or 3:3:2:2 or 3:1:3:3 or 4:2:1:3 or 2:3:4:1, and the freeze-dried powder concentration is 10 9-10 10 CFU / g.

[0063] Preferably, in step S6, the anaerobic fermentation conditions are: inoculation amount of 0.5-1.0 wt%, fermentation temperature of 28° C.-42° C., liquid volume of 60.0-85.0 wt%, and fermentation time of 12-36 h.

[0064] Preferably, in step S7, the polyol is butanediol.

[0065] Preferably, in step S8, the sterilization temperature of the finished filtrate is 65-75° C., and the sterilization time is 30-60 min.

[0066] In this embodiment, before fermentation begins, the interior of the fermentation tank 1 can be flushed and cleaned by the liquid addition pipe 5 and the discharge port 4 to ensure that the fermentation tank 1 is clean;

[0067] After removing impurities, the Radix Scutellariae, Radix Sophorae Flavescentis, Radix Glycyrrhizae, Herba Portulacae and Gentianae are respectively put into different grinding barrels 171 from the feeding hoppers 174 at different positions. The grinding motor 173 is started to drive the grinding table 172 to cooperate with the grinding teeth provided at the bottom of the grinding barrel 171 to grind the raw materials. The raw materials flow into the feeding mechanism 16 under their own weight and are fed into the feeding barrel 14 by the feeding mechanism 16. The increase of the raw materials inside the feeding barrel 14 causes the weight of the feeding barrel 14 to change, and the weighing device 13 obtains data in real time. It is explained here that the weighing device 13 uses the JH-LFB1 spoke-type tension and pressure weighing sensor which can be purchased directly on the market. The weighing device 13 can well measure the weight of the feeding barrel 14. 4 is limited on the central axis to prevent the feeding barrel 14 from tilting, and at the same time, the weight change data of the feeding barrel 14 can be accurately obtained. After the weight reaches the set parameter, the feeding mechanism 16 stops running. It is explained here that the feeding mechanism 16 can be an auger-type feeding mechanism. The material feeding and stopping can be achieved by controlling the rotation start and stop of the auger. It is also a common equipment that can be purchased directly on the market. The solenoid valve 15 is opened, and the raw materials fall into the fermentation tank 1 under their own weight. Because the parameters set for each weighing device 13 are inconsistent, the weight of the materials fed into the fermentation tank 1 by each grinding mechanism 17 and the feeding mechanism 16 is inconsistent, thereby achieving the purpose of feeding various raw materials into the fermentation tank 1 in proportion;

[0068] After the raw materials are put into the fermentation tank 1, water is introduced into the fermentation tank 1 through the liquid adding pipe 5 for soaking. During the soaking process, the stirring mechanism 9 can be used to stir and mix the interior. It is explained here that the stirring mechanism 9 is installed in an eccentric manner, that is, the stirring blades of the stirring mechanism 9 are not on the central axis of the fermentation tank 1. This method can prevent the stirring from forming a vortex and can cooperate well with the gap of the fork plate 112 to prevent interference with the closing mechanism 11. The function of the closing mechanism 11, as the name implies, is to block the feed port 10 to prevent the gas in the stirring, fermentation and mixing process from being discharged from the feed port 10. At the same time, it also makes the interior of the fermentation tank 1 closed to prevent external factors from interfering with the internal fermentation environment. The operating principle of the closing mechanism 11 is as follows: , the servo motor 116 drives the circular gear 115 to rotate, and then the circular gear 115 drives the gear ring 113 to rotate. Because the outer edge of the baffle 114 is limited by the inner wall of the fermentation tank 1, the gear ring 113 will not be out of engagement with the circular gear 115. At the same time, under the obstruction of the fork plate 112, it will not fall down under its own weight, ensuring the engagement effect. The rotation of the gear ring 113 causes the baffle 114 to move to the lower side of the feeding port 10 to achieve closing. Conversely, when the servo motor 116 rotates in the opposite direction, it is opened. It should be noted that the number of rotations of the servo motor 116 is limited by the number of feeding ports 10. The larger the number, the fewer the number of rotations, and the smaller the movement angle of the baffle 114.

[0069] After soaking, the compound enzyme preparation is added through the liquid adding pipe 5 for enzymolysis, and the internal liquid is heated through the heating pipe 6 to achieve the effect of sterilization and enzyme inactivation to obtain the fermentation substrate.

[0070] After the compound lactobacillus freeze-dried powder is inoculated through the liquid adding pipe 5, anaerobic fermentation is carried out, and a 100-mesh bag filter cloth is wrapped or installed at the output end of the discharge port 4. The raw material is discharged and the coarse filtrate is obtained. It should be noted that, although not marked in the figure, the discharge port 4 is also equipped with a valve body, or the corresponding discharge pipe is equipped with a valve body to achieve the non-loss of raw materials during the fermentation process and the discharge of the finished product after the fermentation is completed. This is a conventional design, which is also not difficult to understand and implement for those skilled in the relevant art.

[0071] The above is the operating principle of a device for preparing a composite fermentation liquid of Scutellaria baicalensis.

[0072] like Figures 5 to 8 Shown: A method for preparing a composite fermentation liquid of Scutellaria baicalensis.

[0073] Preparation Example: Preparation of Scutellaria baicalensis compound fermentation broth

[0074] After removing impurities, the medicinal materials of scutellaria baicalensis, sophora flavescens, licorice, purslane, and gentian are put into the preparation device, and the ratio of scutellaria baicalensis, sophora flavescens, licorice, purslane, and gentian is set to 3:2:1:2:2 through the control console 3, and the grinding fineness is 80 mesh. It should be noted here that scutellaria baicalensis, sophora flavescens, licorice, purslane, and gentian are the medicinal parts of medicinal plants specified in the 2020 edition of the Pharmacopoeia of the People's Republic of China;

[0075] The crushed medicinal materials were soaked in 60°C water for 4 hours, with a solid-liquid ratio of 1:10. After the soaking, a composite enzyme preparation was added for enzymatic hydrolysis. The enzymatic hydrolysis temperature was 50°C and the enzymatic hydrolysis time was 4 hours. After the enzymatic hydrolysis, the liquid was heated to 90°C and sterilized for 30 minutes.

[0076] After the liquid temperature dropped to 40°C, a mixed bacterial powder made of freeze-dried bacterial powders of Lactobacillus plantarum, Lactobacillus bulgaricus, Lactobacillus casei, and Bifidobacterium infantis in a ratio of 3:3:2:2 was inoculated. The inoculation amount was 1.0wt%, the fermentation temperature was 39°C, the liquid volume was 75.0wt%, and the fermentation time was 36h.

[0077] After the fermentation is completed, the fermentation liquid is bag filtered to obtain a coarse filtrate, 10.0 wt% butanediol is added to the coarse filtrate to prepare it, and after preparation, it is filtered through a microporous cross-flow membrane and then allowed to stand for 3 hours to obtain a clarified Scutellaria baicalensis composite fermentation liquid;

[0078] The scutellaria baicalensis compound fermentation liquid was filled into a 1 L aluminum bottle and sterilized by heating at 70° C. for 40 min to obtain a finished product of the scutellaria baicalensis compound fermentation liquid.

[0079] Comparative Example: Preparation of Scutellaria baicalensis composite aqueous extract

[0080] After removing impurities, the medicinal materials of Scutellaria baicalensis, Sophora flavescens, Glycyrrhiza uralensis, Portulaca oleracea, and Gentiana scabra were weighed in a ratio of 3:2:1:2:2, mixed and pulverized to a fineness of 80 mesh. The pulverized medicinal materials were soaked in 60°C water for 4 hours with a solid-liquid ratio of 1:10. After the soaking, a composite enzyme preparation was added for enzymatic hydrolysis at a temperature of 50°C for 4 hours. After the enzymatic hydrolysis, the liquid was heated to 90°C and sterilized and enzyme-inactivated for 30 minutes.

[0081] After the temperature of the feed liquid drops to 45° C., the feed liquid is bag-filtered to obtain a coarse filtrate, 10 wt % of butanediol is added to the coarse filtrate to prepare it, and after preparation, the mixture is filtered through a microporous cross-flow membrane to obtain a clarified Scutellaria baicalensis composite water extract;

[0082] The scutellaria baicalensis compound water extract was filled into a 1L aluminum bottle and sterilized by heating at 70°C for 40 minutes to obtain the finished scutellaria baicalensis compound water extract:

[0083] Test example: comparative experiment on inhibition of hyaluronidase activity

[0084] Take 0.1mL CaCl2 solution (0.25mmol / L) and 0.5mL hyaluronidase solution (100U / mL) and incubate in a water bath at 37℃ for 20min; add 0.5mL of the test sample solution and continue to incubate for 20min; then add 0.5mL sodium hyaluronate solution (0.5mg / mL), incubate in a water bath at 37℃ for 30min, then take out and place at room temperature for 5min; add 0.1mL NaOH solution (0.4mol / L) and

[0085] Heat 0.5 mL of acetylacetone solution (3.5 mL of acetylacetone dissolved in 50 mL of 1.0 mol / L sodium carbonate solution) in a boiling water bath for 15 minutes, then immediately transfer to an ice-water bath and cool for 5 minutes. Add 1.0 mL of Ehrlich reagent (0.8 g of p-dimethylaminobenzaldehyde dissolved in 15 mL of concentrated hydrochloric acid and 15 mL of anhydrous ethanol) dropwise, dilute with 3.0 mL of anhydrous ethanol, and allow to stand at room temperature for 20 minutes for color development. Measure the absorbance at 540 nm using a spectrophotometer. The hyaluronidase inhibition rate of the sample is calculated as follows:

[0086] Hyaluronidase inhibition rate = [(AB) - (CD)] % / (AB).

[0087] Where: A - absorbance of control solution (acetic acid buffer solution is used instead of sample solution); B - absorbance of control blank solution (acetic acid buffer solution is used instead of sample solution and enzyme solution); C - absorbance of sample solution; D - absorbance of sample blank solution (acetic acid buffer solution is used instead of enzyme solution);

[0088] The scutellaria baicalensis compound water extract and the scutellaria baicalensis compound fermentation liquid were respectively prepared into solutions with different concentrations of 2%-20%, and used as sample solutions for the in vitro hyaluronidase inhibition rate test.

[0089] Get as Figure 6 Line chart of

[0090] Conclusion: The experimental results show that the hyaluronidase inhibition rate increases with increasing concentration. When the concentration of the Scutellaria baicalensis composite aqueous extract solution is 20%, the inhibition rate reaches 76.02%, and the 50% inhibitory concentration is 8.274%. When the concentration of the Scutellaria baicalensis composite fermentation solution is 20%, the inhibition rate reaches 91.42%, and the 50% inhibitory concentration is 5.413%. The experimental results show that, under the same raw material processing ratio, the hyaluronidase inhibition rate of the Scutellaria baicalensis composite fermentation solution is significantly higher than that of conventional aqueous extracts, and the 50% inhibitory concentration is significantly lower. This indicates that the anti-allergic effect of the Scutellaria baicalensis composite fermentation solution is significantly improved compared with conventional Scutellaria baicalensis composite aqueous extracts, and its dosage in products can be appropriately reduced.

[0091] Example: Composition formulated into cream formula and anti-histamine sensitization effect of cream

[0092] The composition was formulated into a cream. Table 1 shows the test cream formula. The cream was prepared according to the following formula to conduct an antihistamine sensitization test.

[0093] Table 1 Test cream formula

[0094]

[0095]

[0096] Ten volunteers in good health were recruited and numbered from 1 to 10. After washing the inner arm, a 1% histamine solution was applied 15 minutes later. A moderate-positive reaction was observed (-) indicating no reaction, (±) indicating a questionable positive (mild erythema), (+) indicating a weak positive (erythema, infiltrate, and possibly a few papules), (++) indicating a moderate-positive (erythema, infiltrate, papules, and blisters), and (+++) indicating a strong-positive (erythema, infiltrate, papules, and bullae). Cream was then applied to the right inner arm. The skin condition was observed and recorded 0.5, 1, 1.5, 2, 2.5, 3, 3.5, and 4 hours after the test.

[0097] Table 2. Experimental record of antihistamine sensitization by applying cream with 10% Scutellaria baicalensis compound fermentation liquid

[0098]

[0099]

[0100] The volunteers actually used the picture as follows Figure 7 、 Figure 8 ;

[0101] From Table 2 and Figure 7 、 Figure 8 The data from the actual usage comparison chart shows that when the Scutellaria baicalensis compound fermentation liquid is added at a dosage of 10%, it has a significant redness-removing and soothing effect on the volunteers' skin, and can quickly remove redness and relieve itching within 0.5h-2h.

[0102] The above embodiments are merely illustrative of the principles and effects of the present invention and are not intended to limit the present invention. Anyone skilled in the art may modify or alter the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or alterations made by one of ordinary skill in the art without departing from the spirit and technical principles disclosed herein are intended to be covered by the claims of the present invention.

Claims

1. A device for preparing a scutellaria baicalensis composite fermentation liquid, comprising a fermentation tank (1), wherein the fermentation tank (1) is equipped with support legs (2), a control console (3), a discharge port (4), a liquid adding pipe (5) and a heating pipe (6), and the fermentation tank (1) is equipped with a liquid level meter (7) and a thermometer (8), characterized in that: The fermentation tank (1) is equipped with a stirring mechanism (9), a plurality of feeding ports (10) are provided in a ring shape on the top of the fermentation tank (1), a closing mechanism (11) is provided on the fermentation tank (1) and a bracket (12) is provided on the top of the fermentation tank (1) and the brackets (12) are provided in positions and numbers corresponding to the feeding ports (10), a weighing device (13) is fixedly installed on the bracket (12), a feeding cylinder (14) is placed at the center of the weighing device (13), a solenoid valve (15) is provided on the feeding cylinder (14), a feeding mechanism (16) is provided on the input end of the feeding cylinder (14), a grinding mechanism (17) is provided on the input end of the feeding mechanism (16), a supporting frame (18) is provided on the feeding mechanism (16), and a supporting seat (19) is provided on the supporting frame (18).

2. The device for preparing a composite fermentation liquid of Scutellaria baicalensis according to claim 1, characterized in that: The stirring mechanism (9) is installed in such a way that the working end is eccentric to the central axis of the fermentation tank (1).

3. The device for preparing a composite fermentation liquid of Scutellaria baicalensis according to claim 2, characterized in that: The closing mechanism (11) includes a mounting shaft (111), the mounting shaft (111) is equipped with a fork plate (112), a gear ring (113) is placed between the fork plate (112) and the fermentation tank (1), and baffles (114) corresponding in number to the feed inlet (10) are fixedly mounted on the outer side of the gear ring (113), the outer edges of the baffles (114) contact the inner wall of the fermentation tank (1), the gear ring (113) is meshed with a circular gear (115), and the fermentation tank (1) is equipped with a servo motor (116), and the output end of the servo motor (116) is connected to the central axis of the circular gear (115).

4. The device for preparing a composite fermentation liquid of Scutellaria baicalensis according to claim 3, characterized in that: The grinding mechanism (17) comprises a grinding barrel (171), the bottom of the grinding barrel (171) is in an inverted cone shape, the bottom of the grinding barrel (171) is provided with grinding teeth, a grinding table (172) is matched and installed in the area of the grinding barrel (171) provided with the grinding teeth, a grinding motor (173) is connected to the power end of the grinding table (172), and the grinding barrel (171) is provided with a hopper (174).

5. A method for preparing a scutellaria baicalensis compound fermentation liquid, comprising the apparatus for preparing a scutellaria baicalensis compound fermentation liquid according to claims 1 to 4, characterized in that: The method comprises the following preparation steps: S1, putting the medicinal materials of scutellaria baicalensis, sophora flavescens, liquorice, purslane and gentian into the grinding mechanism (17) after removing impurities; S2, starting the grinding mechanism (17) and the feeding mechanism (16), and cooperating with the weighing device (13) to feed the raw materials into the fermentation tank (1) in proportion, wherein the mixing ratio of scutellaria baicalensis, sophora flavescens, liquorice, purslane and gentian is 3-5:1-3:1-2:2-3:1-3; S3, introducing 40-60°C water into the fermentation tank (1) through the liquid adding pipe (5) for soaking for 1-4 hours, with a soaking solid-liquid ratio of 1:8-1:15; S4, adding a complex enzyme preparation for enzymatic hydrolysis; S5. Heating the liquid to 85-95° C., sterilizing and inactivating the enzyme for 20-30 min to obtain a fermentation substrate; S6, inoculating compound lactobacillus freeze-dried powder and performing anaerobic fermentation; S7, using a 100 mesh bag filter to separate and obtain a coarse filtrate, adding 8.0 to 15.0 wt% of polyol and stirring to mix evenly, and letting it stand for 2 to 4 hours after the mixing is completed; S8. After the standing period, the mixed solution is filtered through a microporous cross-flow membrane, and the filtrate is packaged and sterilized to finally obtain the finished product of the Scutellaria baicalensis composite fermentation liquid.

6. The method for preparing a composite fermentation broth of Scutellaria baicalensis according to claim 5, characterized in that: In step S4, the complex enzyme preparation used for enzymatic hydrolysis is a complex enzyme preparation composed of cellulase, medium-temperature amylase, and papain, the ratio of which is 2-5:1-2:1-3, the enzyme dosage is 1.0-3.0 wt%, the enzymatic hydrolysis temperature is 45-55° C., and the enzymatic hydrolysis time is 1-4 hours.

7. The method for preparing a composite fermentation broth of Scutellaria baicalensis according to claim 5, characterized in that: In step S6, the composite lactobacillus freeze-dried powder is prepared by mixing freeze-dried powders of Lactobacillus plantarum, Lactobacillus bulgaricus, Lactobacillus casei and Bifidobacterium infantis in a ratio of 4:1:2:3 or 3:3:2:2 or 3:1:3:3 or 4:2:1:3 or 2:3:4:1, and the freeze-dried powder concentration is 10 9 -10 10 CFU / g.

8. The method for preparing a composite fermentation broth of Scutellaria baicalensis according to claim 5, characterized in that: In step S6, the anaerobic fermentation conditions are: inoculation amount of 0.5-1.0 wt%, fermentation temperature of 28° C.-42° C., liquid volume of 60.0-85.0 wt%, and fermentation time of 12-36 h.

9. The method for preparing a composite fermentation broth of Scutellaria baicalensis according to claim 5, characterized in that: In step S7, the polyol is butanediol.

10. The method for preparing a composite fermentation broth of Scutellaria baicalensis according to claim 5, characterized in that: In step S8, the sterilization temperature of the finished filtrate is 65-75° C., and the sterilization time is 30-60 min.

Images