A strain of Pediococcus pentosaceus and its application

Abstract

The present invention discloses a strain of Pediococcus pentosaceus and its application. It belongs to the technical field of conversion of effective ingredients. Based on the total saponin and polysaccharide content in American ginseng, the present invention screened a lactic acid bacterium that efficiently converts saponins and polysaccharides using a comprehensive score as an indicator. The strain was identified as Pediococcus pentosaceus through morphological, physiological, biochemical, and molecular biological identification and deposited in the General Microbiology Center of the China Microorganism Culture Collection Administration with the deposit number CGMCC No. 33430. After the transformation, the total saponin content in American ginseng and ginseng increased by 53.90% and 209.61%, respectively, and the polysaccharide content increased by 124.68% and 68.16%, respectively. After the transformation, the DPPH antioxidant capacity, ABTS antioxidant capacity, blood sugar lowering capacity, and blood lipid lowering capacity of the total saponin extracts and polysaccharide extracts of American ginseng and ginseng were all improved. The Pediococcus pentosaceus screened by the present invention has broad application prospects.

Description

Technical Field

[0001] The present invention relates to the technical field of functional component transformation, and more particularly to a Pediococcus pentosaceus strain and application thereof. Background Art

[0002] Ginseng and American ginseng have a long history of consumption in my country. Currently, my country's total ginseng production reaches 90,000 tons, and American ginseng production exceeds 10,000 tons. Both are abundant and possess significant medicinal benefits, such as antioxidant and blood sugar-lowering properties. my country added ginseng and American ginseng to the list of medicinal and edible herbs in 2014 and 2023, respectively, propelling their development into a new era.

[0003] Ginseng and American ginseng contain dietary fiber, starch, protein, polysaccharides, and saponins, among which saponins and polysaccharides are the main active ingredients. However, research has found that the content of these active ingredients in ginseng and American ginseng is relatively low, and some rare saponins are not even present in natural ginseng and American ginseng. This has greatly hindered the development and utilization of ginseng and American ginseng. Therefore, the conversion of active ingredients from ginseng and American ginseng has become a research hotspot and an urgent issue to be addressed for industrialization.

[0004] Currently, methods for converting active ingredients can be categorized into physical, chemical, and biological conversion methods. Bioconversion offers advantages such as ease of use, a wide variety of options, strong targeting, and high conversion efficiency. Therefore, bioconversion is the primary approach for converting active ingredients. Microbial conversion can produce rare saponins by cleaving the glycosidic bonds within saponins through the production of glycoside hydrolases. Ginseng and American ginseng can also convert carbohydrates such as starch and dietary fiber into polysaccharides through microbial respiration, further enhancing their therapeutic value. Among the many microorganisms, lactic acid bacteria are the preferred choice for bioconversion due to their high safety and excellent conversion performance. However, few lactic acid bacteria strains have been reported that can efficiently convert the active ingredients of ginseng or American ginseng.

[0005] In summary, how to provide a lactic acid bacterium that can efficiently convert saponins and polysaccharides is an urgent problem that needs to be solved by those skilled in the art. Summary of the Invention

[0006] In view of this, the present invention provides a Pediococcus pentosaceus strain and applications thereof.

[0007] The purpose of the present invention is to screen for dominant lactic acid bacteria strains capable of converting saponins and polysaccharides and apply them to the conversion of the active ingredients of ginseng, American ginseng, and other products. Based on cost-effectiveness considerations, the present invention first selects American ginseng as a raw material to identify lactic acid bacteria strains with high conversion efficiency. The optimal conversion process is determined using total saponin and polysaccharide content as evaluation indicators. American ginseng and ginseng are then converted using the optimized conversion process, and the composition of the conversion products is analyzed and the efficacy of the conversion products is evaluated.

[0008] In order to achieve the above object, the present invention adopts the following technical solutions:

[0009] A strain of Pediococcus pentosaceus, named JL-RS13, and classified as Pediococcus pentosaceus, was deposited in the General Microbiology Center of the China Culture Collection of Microorganisms on January 16, 2025, with a deposit number of CGMCC No. 33430, and a deposit address of the Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing.

[0010] The application of the above-mentioned Pediococcus pentosaceus in the fermentation and conversion of ginsenosides and polysaccharides.

[0011] Furthermore, the ginseng is Panax ginseng or American ginseng.

[0012] Furthermore, it is used to increase the content of saponin and polysaccharide.

[0013] Furthermore, it is used to improve antioxidant capacity, blood sugar lowering capacity and blood lipid lowering capacity.

[0014] The application of the above-mentioned Pediococcus pentosaceus in the fermentation preparation of ginseng / American ginseng lactic acid bacteria beverage.

[0015] A bacterial agent for converting saponins and polysaccharides comprises the above-mentioned Pediococcus pentosaceus.

[0016] A method for increasing saponins and polysaccharides in ginseng / American ginseng, using the above-mentioned Pediococcus pentosaceus.

[0017] It can be seen from the above technical solutions that, compared with the prior art, the present invention has the following beneficial effects:

[0018] The present invention screened a lactic acid bacterium that efficiently converts saponins and polysaccharides based on the total saponin and polysaccharide content in American ginseng using a comprehensive scoring index. The bacterium was identified as Pediococcus pentosaceus through morphological, physiological, biochemical, and molecular biological analysis and deposited with the General Microbiology Center of the China Microorganism Culture Collection Administration under the deposit number CGMCC No. 33430. After transformation, the total saponin content in American ginseng and Panax ginseng increased by 53.90% and 209.61%, respectively, and the polysaccharide content increased by 124.68% and 68.16%, respectively. The total saponin extracts and polysaccharide extracts of American ginseng and Panax ginseng after transformation showed improved DPPH antioxidant capacity, ABTS antioxidant capacity, blood sugar lowering capacity, and blood lipid lowering capacity. The Pediococcus pentosaceus screened by the present invention has broad application prospects. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are merely embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the provided drawings without paying any creative work.

[0020] Figure 1 This is the colony morphology of strain JL-RS13 in Example 2 of the present invention;

[0021] Figure 2 The cell morphology of the strain JL-RS13 after staining in Example 2 of the present invention is shown;

[0022] Figure 3 This is an electrophoresis diagram of the PCR product of strain JL-RS13 in Example 2 of the present invention;

[0023] Figure 4 This is the phylogenetic tree of strain JL-RS13 in Example 2 of the present invention;

[0024] Figure 5 The results of the single factor test on the particle size of American ginseng in Example 4 of the present invention are shown, where a represents the polysaccharide and saponin content, and b represents the comprehensive score.

[0025] Figure 6 The results of the single-factor test of the inoculum amount of American ginseng in Example 4 of the present invention are shown in Table 1, where a represents the polysaccharide and saponin content, and b represents the comprehensive score result.

[0026] Figure 7 The results of the single-factor test on the cultivation temperature of American ginseng in Example 4 of the present invention are shown in Figure 4, where a represents the polysaccharide and saponin content, and b represents the comprehensive score result.

[0027] Figure 8 The results of the single-factor test of American ginseng cultivation time in Example 4 of the present invention are shown, where a represents the polysaccharide and saponin content results, and b represents the comprehensive score result;

[0028] Figure 9 This is a liquid phase diagram of American ginseng before and after transformation in Example 5 of the present invention;

[0029] Figure 10 This is a liquid phase diagram of ginseng before and after transformation in Example 5 of the present invention. DETAILED DESCRIPTION

[0030] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0031] The reagents required for the present invention are conventional experimental reagents, purchased from commercial channels; the experimental methods not mentioned are conventional experimental methods and will not be described in detail here.

[0032] Example 1

[0033] Strain screening

[0034] 1. Activation and expansion of lactic acid strains

[0035] Lactic acid bacteria strains screened and preserved in the Agricultural Products Processing and Storage Engineering Laboratory of the College of Food Science and Engineering of Jilin Agricultural University (isolated from kimchi, cheese, yogurt and other foods, numbered JL-RS1, JL-RS2, ..., JL-RS14) were inoculated into MRS medium at a 1% inoculum volume and cultured in a shaking incubator at 37 °C and 180 rpm for 24 h to obtain the activated bacterial solution (OD 600 The activated bacterial solution was inoculated into MRS medium at a 2% inoculum volume and cultured in a shaking incubator at 37°C and 180 rpm for 24 h to obtain the expanded bacterial solution (OD 600 Activation is complete when the value reaches 1.8).

[0036] 2. Raw material pretreatment

[0037] Commercially available American ginseng was coarsely ground in a food processor to obtain coarse particles with a diameter of 0.5-1 cm. The coarse particles were further ground and sieved in an ultrafine grinder to obtain American ginseng powder with a mesh size of 100-150.

[0038] 3. Fermentation Broth Preparation

[0039] The American ginseng powder was diluted with a solid-liquid ratio of 1:10 to obtain an American ginseng fermentation broth. The broth was sterilized in an autoclave at 121°C for 20 minutes to prevent contaminants in the fermentation broth from affecting the test results.

[0040] 4. Microbial transformation treatment

[0041] The culture solution described in step 1 was inoculated into the American ginseng fermentation liquid at a 10% inoculum rate, placed in a shaking incubator at 180 r / min, and cultured at 37°C for 7 days to obtain the American ginseng transformation liquid.

[0042] 5. Determine the optimal transformed lactic acid bacteria strain

[0043] (1) Screening method for optimal transformed lactic acid bacteria strains

[0044] The American ginseng transformation liquid was removed, sterilized at 121°C for 20 minutes, and freeze-dried to obtain the American ginseng transformation product. The total saponin and polysaccharide contents of the transformation products from different strains were compared. The weights of total saponins and polysaccharides were determined using the entropy method. A comprehensive score for the transformation effect of each strain was calculated, and the strain with the best transformation effect was selected using this comprehensive score.

[0045] (2) Comprehensive score calculation method

[0046] (1)

[0047] Among them, R in Y is the normalized result of the ith test of the nth indicator (e.g., the normalized result of the first test of the saponin indicator in the strain screening test); in is the value of the i-th sample indicator of the n-th indicator; Y imax is the maximum value of the nth index; Y imin is the minimum value of the nth indicator.

[0048] (2)

[0049] Among them, V in is the non-negative translation result of the i-th sample of the n-th indicator.

[0050] (3)

[0051] Among them, P in is the proportion of the i-th sample on the n-th indicator, and m is the number of samples.

[0052] (4)

[0053] Among them, E n is the entropy value of the nth indicator.

[0054] (5)

[0055] Among them, W n is the weight of the nth indicator; j is the number of indicators.

[0056] (6)

[0057] Among them, U in It is the comprehensive score of the nth indicator Y1 and Y2.

[0058] 6. Filter results

[0059] The 14 lactic acid bacteria strains described above were screened, and the resulting conversion effects are shown in Table 1. Among them, JL-RS13 had the strongest conversion capacity for total saponins and polysaccharides (total saponin content increased by 45.83% and polysaccharide content increased by 100.70% after conversion) and the highest overall score (0.9799).

[0060]

[0061] Example 2

[0062] Strain identification

[0063] 1. Morphological identification: After activation, strain JL-RS13 was inoculated on MRS solid plates for culture and observation of colony morphology; strain JL-RS13 was subjected to Gram staining to observe bacterial cell morphology.

[0064] Depend on Figure 1 、 Figure 2 Strain JL-RS13 is a round, smooth, moist colony with a central, upwardly bulging center and neatly margined, milky-white colonies. The cells are spherical, arranged in tetrads or sheets, and do not form spores. Gram staining is purple and positive.

[0065] 2. Physiological and biochemical identification

[0066] The strain JL-RS13 was subjected to catalase reaction, hydrogen sulfide, gelatin liquefaction, indole production, VP test and carbohydrate utilization test, and the results were comprehensively evaluated against the "Classification, Identification and Experimental Methods of Lactic Acid Bacteria".

[0067] (1) Catalase reaction: Inoculate strain JL-RS13 onto MRS slant medium and culture at 37°C for 24 h. Then, add a few drops of hydrogen peroxide solution and observe whether bubbles are generated.

[0068] (2) Hydrogen sulfide experiment: strain JL-RS13 was inoculated into trisaccharide iron agar medium and cultured at 20°C for 7 days to observe whether it turned black.

[0069] (3) Gelatin liquefaction experiment: The strain JL-RS13 was inoculated into a gelatin culture medium test tube and cultured at 20°C to observe the growth of the strain and whether the gelatin melted.

[0070] (4) Indole test: strain JL-RS13 was inoculated into peptone water culture medium and cultured at 37 °C for 24 h. Ether and indole reagent were then added. If the liquid layer interface turned rose-colored, it was positive; otherwise, it was negative.

[0071] (5) VP test: Inoculate strain JL-RS13 into a glucose-peptone water medium and incubate at 37°C for 24 h. Add VP reagent I and mix thoroughly. Add VP reagent II and mix again. If a pink to red ring appears within 5–15 min, the test is positive; otherwise, it is negative.

[0072] (6) Carbohydrate utilization experiment: strain JL-RS13 was inoculated into various carbohydrate culture tubes and cultured at 37 °C for 24 h to observe the utilization.

[0073] The physiological and biochemical identification results of strain JL-RS13 are shown in Table 2. The strain tested negative for indole, VP, hydrogen sulfide, catalase, and gelatin liquefaction. It was able to utilize glucose, maltose, and rhamnose, but not lactose or mannitol. Furthermore, it fermented glucose to produce acid but not gas. This strain was preliminarily identified as a lactic acid bacterium.

[0074]

[0075] 3.16S rDNA molecular biological identification

[0076] The genome of strain JL-RS13 was extracted using the Omega Bacterial DNA Kit (Model: D3350). PCR amplification was performed using Vazyme's 2× Taq Master Mix. The PCR reaction system and conditions are shown in Table 3.

[0077]

[0078] The strain JL-RS13 was amplified by PCR and then analyzed by agarose gel electrophoresis. Figure 3 shown.

[0079] Sequencing was performed using an ABI DNA sequencer (model: 3730XL). The gene sequence of strain JL-RS13 is as follows:

[0080]

[0081] Sequence alignment was performed using NCBI, and a phylogenetic tree was constructed using the maximum likelihood (ML) method. The results are shown in Figure 4 , strain JL-RS13 is in the same branch as Pediococcus pentosaoeus.

[0082] Combining phenotypic identification such as morphology, physiological and biochemical indicators and molecular biological identification results, strain JL-RS13 was identified as Pediococcus pentosaceus.

[0083] Example 3

[0084] strain collection

[0085] Pediococcus pentosaceus JL-RS13, whose classification name is Pediococcus pentosaceus, was deposited in the General Microbiology Center of China Culture Collection of Microorganisms on January 16, 2025, with the deposit number CGMCC No. 33430, and the deposit address is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing.

[0086] Example 4

[0087] Optimization of the optimal transformation process of strain JL-RS13

[0088] 1. Activation and expansion of strain JL-RS13

[0089] The specific operation is the same as that in Example 1.

[0090] 2. Raw material pretreatment

[0091] Commercially available American ginseng was coarsely ground in a food processor to obtain coarse particles with a diameter of 0.5-1 cm. The coarse particles were further ground and sieved in an ultrafine grinder to obtain American ginseng powder with mesh sizes of 50-100, 100-150, 150-200, 200-250, and 250-300.

[0092] 3. Fermentation Broth Preparation

[0093] The specific operation is the same as that in Example 1.

[0094] 4. Determine the optimal conversion process and parameters

[0095] (1) Single-factor experiment

[0096] The culture solution described in step 1 was inoculated into the American ginseng fermentation broth, and single-factor experiments were performed with the crushing particle size (50-100, 100-150, 150-200, 200-250, 250-300 mesh), inoculation amount (the fraction of the total fermentation liquid volume after inoculation: 6, 8, 10, 12, 14%), incubation temperature (31, 34, 37, 40, 43 ℃), and incubation time (5, 6, 7, 8, 9 d) as factors.

[0097] The results of single factor evaluation are as follows Figures 5 to 8 As shown in the figure, according to the comprehensive score and considering the economic factors, each factor obtained three significant levels for the next step of orthogonal optimization, and three groups of factors were selected for orthogonal optimization: the crushing particle size of 50-100, 100-150, 150-200 mesh, the inoculation amount of 6, 8, 10%, the incubation temperature of 34, 37, 40℃, and the incubation time of 5, 6, and 7d.

[0098] (2) Orthogonal experiment

[0099] Based on the results of the single-factor experiment, a four-factor, three-level orthogonal experiment was conducted with particle size (A), inoculation size (B), incubation temperature (C), and incubation time (D) as factors. The transformed American ginseng transformation liquid was removed, sterilized at 121°C for 15-20 minutes, and freeze-dried to obtain the American ginseng transformation product. The total saponin and polysaccharide content of the American ginseng transformation product was determined, and a comprehensive score was calculated to determine the optimal transformation process and parameters for strain JL-RS13. The comprehensive score calculation method was the same as in Example 1.

[0100]

[0101] Table 4 shows that among the factors influencing the conversion of American ginseng's active ingredients, incubation temperature has the greatest impact, followed by incubation time, then inoculum size, and the smallest influence is particle size. Based on the K value, the optimal conversion is A2B1C2D2, but this does not appear in the results analysis table. Furthermore, the orthogonal experiment and results analysis revealed that A3B1C2D2 had the highest overall score, necessitating verification testing of both groups.

[0102]

[0103] Table 5 shows that A2B1C2D2 had the highest conversion capacity in the validation test. Therefore, the optimal conversion conditions for the active ingredients of American ginseng were A2B1C2D2: 100-150 mesh particle size, 6% inoculation, 37°C incubation temperature, and 6 days of incubation. The polysaccharide content of American ginseng increased from 96.47±1.53 mg / g to 216.75±2.48 mg / g, a 124.68% increase. The total saponin content of American ginseng increased from 36.05±0.28 mg / g to 55.48±0.54 mg / g, a 53.90% increase.

[0104] Example 5

[0105] Comparative test on the content of ingredients of American ginseng and ginseng before and after transformation

[0106] 1. Activation and expansion of strain JL-RS13

[0107] The specific operation is the same as that in Example 1.

[0108] 2. Raw material pretreatment

[0109] Commercially available American ginseng and ginseng were coarsely ground in a food processor to obtain coarse particles with a diameter of 0.5 to 1 cm. The coarse particles were transferred to an ultrafine grinder for further grinding and sieving to obtain 100 to 150 mesh American ginseng powder and ginseng powder.

[0110] 3. Fermentation Broth Preparation

[0111] American ginseng powder and ginseng powder were diluted at a solid-liquid ratio of 1:10 to obtain American ginseng fermentation liquid and ginseng fermentation liquid. The liquid was sterilized in an autoclave at 121°C for 20 minutes to prevent contaminants in the fermentation liquid from affecting the test results.

[0112] 4. Transformation of American ginseng and Panax ginseng by strain JL-RS13

[0113] Inoculate the culture from step 1 into the American ginseng and ginseng fermentation broths, respectively, at an inoculum size of 6%, an incubation temperature of 37°C, and a 6-day incubation period. After transformation is complete, remove the transformed American ginseng and ginseng fermentation broths and sterilize them at 121°C for 20 minutes.

[0114] 5. Determination of lactic acid content

[0115] The American ginseng conversion solution and the Panax ginseng conversion solution were each ultrasonically treated at 150 W for 20 minutes, followed by centrifugation at 4000 rpm for 20 minutes. The supernatants were collected to obtain the American ginseng conversion solution and the Panax ginseng conversion solution, respectively, for testing. Lactic acid content was determined using GB 12456-2021. The lactic acid content in the American ginseng conversion solution was 6.9 ± 0.2 g / L, and the lactic acid content in the Panax ginseng conversion solution was 6.8 ± 0.3 g / L.

[0116] The sterilized American ginseng transformation liquid and ginseng transformation liquid were freeze-dried to obtain American ginseng transformation products and ginseng transformation products, which were used for the following component determinations.

[0117] 6. Determination of dietary fiber content

[0118] The enzymatic hydrolysis method in GB 5009.88-2023 was used to determine the contents of total dietary fiber, soluble dietary fiber, and insoluble dietary fiber.

[0119] 7. Determination of starch content

[0120] The starch content was determined by the enzymatic hydrolysis method in GB 5009.9-2023.

[0121] 8. Protein Content Determination

[0122] The protein content was determined by the Kjeldahl method in GB5009.5-2025.

[0123] 9. Polysaccharide content analysis

[0124] The polysaccharide content was determined by spectrophotometry according to DB 22 / T1685-2012.

[0125] 10. Ash content determination

[0126] The ash content was determined by the combustion method in GB 5009.4-2016.

[0127] 11. Analysis of saponin content

[0128] (1) Determination of total saponin content

[0129] The total saponin content was determined by the spectrophotometric method in DB 22 / T 1668-2012.

[0130] (2) Determination of monomer saponin content

[0131] Extraction: Weigh 1 g (accurate to 0.001 g) of ginseng powder, American ginseng powder, ginseng transformation product, and American ginseng transformation product (ground through a 60-mesh sieve) into a 250 mL Erlenmeyer flask. Add 50 mL of water-saturated n-butanol and soak overnight. The next day, place the Erlenmeyer flask in an ultrasonic cleaner at 50 / 60 Hz, 1500 W, for 30 minutes. Centrifuge at 4000 rpm for 10 minutes, and transfer the supernatant to an evaporating dish. Repeat the extraction process twice for 10 minutes each. Combine the n-butanol solutions and evaporate to dryness at 90°C. Dissolve the evaporated material in methanol several times and dilute to 5 mL. This is the monomer saponin test solution and is set aside.

[0132] Purification: Pipette 2 mL of monomer saponin test solution into a centrifuge tube, centrifuge at 4000 r / min for 10 min, and filter the supernatant through a 0.22 µm filter for later use.

[0133] Chromatographic conditions for determination of monomeric saponins: chromatographic column: C18 column (2.1 mm × 100 mm, 2.6 µm), mobile phase: 0.1% formic acid solution-acetonitrile, column temperature: 40 °C, flow rate: 0.3 mL / min, injection volume: 20.0 µL, gradient elution program is shown in Table 6.

[0134]

[0135] 12. Fat content determination

[0136] The fat content was determined by the acid hydrolysis method in GB5009.6-2016.

[0137] 13. Determination of flavonoid content

[0138] The flavonoids content was determined by spectrophotometry according to SN / T 4592-2016.

[0139]

[0140] Analysis of the main components in Table 7 revealed that the contents of polysaccharides, soluble dietary fiber, protein, total saponins, ash, and flavonoids increased, while the contents of total dietary fiber, insoluble dietary fiber, starch, and fat decreased. Polysaccharide content showed the greatest increase, increasing by 124.68%. Soluble dietary fiber content increased by 37.49%, protein content increased by 6.73%, total saponins content increased by 53.90%, ash content increased by 13.80%, and flavonoid content increased by 25.17%. Total dietary fiber content decreased by 11.30%, insoluble dietary fiber decreased by 23.52%, starch content decreased by 72.88%, and fat content decreased by 8.88%.

[0141] Depend on Figure 9 It can be seen that the peak area and number of peaks increased significantly after the transformation of American ginseng, indicating an increase in the content and variety of saponins. A comparison in Table 7 shows that after the transformation of American ginseng, the content of saponins Rf, Rb1, Rc, Rb2, Rb3, and Rd all increased to varying degrees, while the content of saponins Rg1 and Re decreased slightly. Saponins Rf and Rb2 are essentially absent in American ginseng, and their content increased from 0.0116 mg / g and 0.0181 mg / g to 0.3044 mg / g and 0.6919 mg / g respectively after transformation.

[0142]

[0143] Analysis of the main component contents shown in Table 8 revealed that transformation with strain JL-RS13 increased the polysaccharide, total saponin, ash, and flavonoid contents of ginseng, while decreased the total dietary fiber, insoluble dietary fiber, soluble dietary fiber, starch, protein, and fat contents. Specifically, polysaccharide content increased by 68.16%, ash content increased by 17.05%, total saponin content increased by 209.61%, and flavonoid content increased by 64.29%. Total dietary fiber content decreased by 33.81%, soluble dietary fiber decreased by 15.43%, insoluble dietary fiber content decreased by 43.62%, starch content decreased by 59.05%, protein content decreased by 8.32%, and fat content decreased by 6.56%.

[0144] Depend on Figure 10 As can be seen, the peak area and number of peaks increased significantly after ginseng transformation, indicating an increase in saponin content and variety. A comparison in Table 8 shows that after ginseng transformation, the content of saponins Rg1, Re, Rf, Rb1, Rc, Rb2, and Rb3 all increased to varying degrees, while the content of saponin Rd decreased slightly. The increase in saponin Rb1 was the most significant, increasing from 1.3328 mg / g to 5.8263 mg / g.

[0145] Example 6

[0146] Comparative test on the efficacy of American ginseng and ginseng before and after transformation

[0147] 1. Activation and expansion of strain JL-RS13

[0148] The specific operation is the same as that in Example 1.

[0149] 2. Raw material pretreatment

[0150] The specific operation is the same as that in Example 5.

[0151] 3. Fermentation Broth Preparation

[0152] The specific operation is the same as that in Example 5.

[0153] 4. Transformation of Ginseng and American Ginseng by Strain JL-RS13

[0154] The specific operation is the same as that in Example 5.

[0155] 5. Preparation of Ginseng and American Ginseng Test Solution

[0156] (1) Preparation of ginseng and American ginsenosides test solution

[0157] The extraction process for the total saponin test solutions of ginseng and American ginseng follows DB 22 / T 1668-2012. Dissolve the evaporated material in methanol several times to a volume of 10 mL. This yields the unconverted American ginseng total saponin test solution, the American ginseng conversion product total saponin test solution, the unconverted ginseng total saponin test solution, and the ginseng conversion product total saponin test solution, which are then set aside.

[0158] (2) Preparation of ginseng and American ginseng polysaccharide test solution

[0159] The extraction process for ginseng and American ginseng polysaccharide test solutions was performed according to DB 22 / T1685-2012. The residue was dissolved in water in a 25 mL volumetric flask to obtain unconverted American ginseng polysaccharide test solution, American ginseng conversion product polysaccharide test solution, unconverted ginseng polysaccharide test solution, and ginseng conversion product polysaccharide test solution, which were set aside.

[0160] The test solutions include: unconverted American ginseng total saponin test solution, American ginseng transformation product total saponin test solution, unconverted ginseng total saponin test solution, ginseng transformation product total saponin test solution, unconverted American ginseng polysaccharide test solution, American ginseng transformation product polysaccharide test solution, unconverted ginseng polysaccharide test solution, and ginseng transformation product polysaccharide test solution.

[0161] 6. DPPH free radical scavenging rate determination

[0162] The determination was performed with reference to the method of Mi Zhenzhen in “Analysis of components of ginger essential oil using different extraction methods and analysis of its biological activity”.

[0163]

[0164] As shown in Table 9, the DPPH radical scavenging rates of the American ginseng and ginseng transformation products after transformation by strain JL-RS13 were significantly improved compared with those before transformation.

[0165] 7.ABTS + Free radical scavenging rate assay

[0166] The determination was performed with reference to the method of Mi Zhenzhen in “Analysis of components of ginger essential oil using different extraction methods and analysis of its biological activity”.

[0167]

[0168] As shown in Table 10, the transformation products of American ginseng and Panax ginseng after transformation by strain JL-RS13 were significantly higher than those before transformation by ABTS. + The free radical scavenging rate is significantly improved.

[0169] 8. Determination of blood sugar lowering ability (α-glucosidase inhibition rate)

[0170] The determination was performed with reference to the experimental method described by Liang Liwen in “Study on the Inhibition of α-glucosidase Activity and Improvement of Insulin Resistance by American Ginseng and Red American Ginseng”.

[0171]

[0172] As shown in Table 11, the α-glucosidase inhibition rate of the American ginseng and ginseng transformation products after the transformation of strain JL-RS13 was significantly improved compared with that before transformation.

[0173] 9. Determination of blood lipid lowering ability (pancreatic lipase inhibition rate)

[0174] The determination was performed with reference to the experimental method of Fan Shengyu in “Effects of lactic acid bacteria fermentation on the structure and biological activity of kelp fucoidan”.

[0175]

[0176] As shown in Table 12, the pancreatic lipase inhibition rate of the American ginseng and ginseng transformation products after the transformation of strain JL-RS13 was significantly improved compared with that before transformation.

[0177] Example 7

[0178] Development of American ginseng lactic acid beverage

[0179] A ginseng conversion stock solution was prepared using the method described in Example 5. A ginseng lactic acid beverage was developed using this ginseng conversion stock solution. The beverage contained 10% ginseng conversion stock solution, 10% soluble solids, and 2.5 g / L lactic acid. The beverage had a total saponin content of 43.81 mg / 100 mL and a polysaccharide content of 183.80 mg / 100 mL. The resulting ginseng lactic acid beverage had a pleasant taste, a moderate sweet and sour flavor, and possessed the characteristic aroma of ginseng.

[0180] The various embodiments in this specification are described in a progressive manner, and each embodiment focuses on the differences from other embodiments. The same or similar parts between the various embodiments can be referenced to each other.

[0181] The above description of the disclosed embodiments is intended to enable one skilled in the art to implement or use the present invention. Various modifications to these embodiments will be readily apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention is not limited to the embodiments shown herein but is intended to conform to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. Application of Pediococcus pentosaceus in fermentation and conversion of ginsenosides and polysaccharides, characterized in that: Used to increase the content of total saponins, Rf, Rb1, Rc, Rb2, Rb3 and polysaccharides; Used to improve antioxidant capacity, blood sugar lowering capacity and blood lipid lowering capacity; The Pediococcus pentosaceus is named JL-RS13, and its classification name is Pediococcus pentosaceus. It has been deposited in the General Microbiology Center of China Culture Collection of Microorganisms on January 16, 2025, with the deposit number CGMCC No. 33430, and the deposit address is Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing; The ginseng genus is Panax ginseng or American ginseng.

2. Use of the Pediococcus pentosaceus according to claim 1 in fermenting ginseng or American ginseng lactic acid bacteria beverage.

3. A bacterial agent for transforming ginseng or American ginseng saponins and polysaccharides, characterized in that: The method comprises the Pediococcus pentosaceus according to claim 1.

4. A method for increasing saponins and polysaccharides in ginseng or American ginseng, characterized in that: The Pediococcus pentosaceus according to claim 1 is used to ferment and transform ginseng or American ginseng.

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