A method for promoting microalgae growth using pharmaceutical products
By adding different types and concentrations of pharmaceutical products in the early stages of microalgae cultivation, the problems of slow microalgae growth and low production efficiency were solved, enabling rapid increase in microalgae biomass and large-scale production, reducing production costs and environmental pollution.
Patent Information
- Application Number
- CN202510953799.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2025-07-11
- Publication Date
- 2025-12-02
- Estimated Expiration
- 2045-07-11
AI Technical Summary
Existing microalgae production processes have low growth rates and low production efficiency, making it difficult to meet the needs of large-scale emission reduction in terms of carbon dioxide fixation. Traditional promotion methods have significant limitations in improving these processes.
Different types and concentrations of pharmaceutical products, such as steroids, fluorine, antibiotics, and non-method steps, are added during the early stages of microalgae cultivation. These include adding steroids, fluoroquinolone antibiotics, sulfonamide antibiotics, or non-steroidal anti-inflammatory drugs during the early stages of Chlorella growth, and promoting microalgae growth through light cultivation.
It significantly increases microalgae biomass, enables large-scale production, reduces production costs, minimizes environmental pollution, and enhances the market competitiveness of microalgae-related products.
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Figure CN120442514B_ABST
Abstract
Description
Technical Field
[0001] This invention belongs to the field of microalgae biology and aquaculture technology, specifically relating to a method for promoting microalgae growth using pharmaceutical products. Background Technology
[0002] Microalgae are diverse and widely distributed on land and in the ocean. They have high photosynthetic efficiency, making efficient use of light energy and absorbing CO2 to convert it into nutrients such as proteins, polysaccharides, and lipids. They have great development prospects in the fields of food, medicine, energy, and environmental protection.
[0003] In the production of high-value-added products from microalgae, growth rate and production efficiency are key factors. However, current production processes and technologies still have certain limitations in microalgae growth and product synthesis, resulting in long production cycles and limited by-product yields. Microalgae absorb carbon dioxide through photosynthesis, but due to their limited growth rate and scale, the amount of carbon dioxide fixed is insufficient to meet the needs of large-scale emission reduction. Accelerating microalgae growth can increase their biomass and photosynthetic efficiency, thereby fixing more carbon dioxide per unit time and making a greater contribution to mitigating the greenhouse effect.
[0004] Traditional methods for promoting microalgae growth, such as optimizing culture conditions like light, temperature, and nutrients, have achieved some success but face limitations in further improvement. Therefore, finding a low-cost, efficient, and environmentally friendly method to promote microalgae growth is of significant practical importance. Summary of the Invention
[0005] To address the shortcomings of the existing technology, this invention provides a method for promoting microalgae growth using pharmaceutical products. By adding different types and concentrations of pharmaceutical products to microalgae, the effects of adding different types and concentrations of pharmaceutical products on promoting microalgae growth are analyzed. This method can rapidly increase the biomass of Chlorella, which is of great significance for the large-scale production of microalgae.
[0006] The specific technical solution is as follows:
[0007] A method for promoting microalgae growth using pharmaceutical products includes the following steps: activating microalgae on a solid plate, inoculating the activated microalgae into a liquid culture medium, adding pharmaceutical products in the early stage of microalgae growth, and culturing in a constant temperature shaker under light.
[0008] Furthermore, the concentration of the pharmaceutical product added is 0.1~30 mg / L.
[0009] Furthermore, the preferred concentration of the pharmaceutical product is 5 mg / L, 10 mg / L, 20 mg / L, or 30 mg / L, more preferably 20 mg / L.
[0010] Furthermore, the initial OD of the microalgae 680 The concentration of the light source is 0.1~0.3, the shaking speed is 80~150 rpm, the light intensity is 1000~3000 lux, and the culture temperature is 25~30℃.
[0011] Furthermore, the pharmaceutical product is one or more of the following: steroids, fluoroquinolone antibiotics, sulfonamide antibiotics, or nonsteroidal anti-inflammatory drugs.
[0012] Furthermore, the steroid drug is prednisone and / or dexamethasone, preferably dexamethasone.
[0013] Furthermore, the fluoroquinolone antibiotic is one or more of norfloxacin, enoxacin, fleroxacin, ofloxacin, ciprofloxacin, or lomefloxacin, preferably norfloxacin.
[0014] Furthermore, the sulfonamide antibiotic is one or more of sulfadiazine, sulfamethoxazole, sulfisoxazole, sulfacetyl or sulfadiazine, preferably sulfisoxazole.
[0015] Furthermore, the nonsteroidal anti-inflammatory drug is one or more of diclofenac, ibuprofen, or indomethacin, preferably indomethacin.
[0016] Furthermore, the microalgae is Chlorella vulgaris, the solid plate is a BG11 solid plate, and the culture medium is a BG11 culture medium.
[0017] Furthermore, the activation includes the following steps: using an inoculation loop to pick up the preserved Chlorella liquid and streak it on a BG11 solid plate; picking up a single algal colony that has grown on the plate and inoculating it into liquid culture medium BG11 for growth; after growing to a dark green color, expanding the culture; the expanded Chlorella is activated Chlorella.
[0018] Compared with the prior art, the beneficial effects of the present invention are as follows:
[0019] (1) By adding low concentrations of different pharmaceutical products to the microalgae culture system, the present invention can effectively promote the growth of microalgae, rapidly increase the biomass of microalgae, and realize the large-scale production of microalgae.
[0020] (2) This invention uses different pharmaceutical products as growth regulators for Chlorella, which effectively increases the biomass of Chlorella and is of great significance for the large-scale production of Chlorella.
[0021] (3) The present invention utilizes a pharmaceutical product to promote the growth of microalgae. When the concentration of the pharmaceutical product is 20 mg / L, it can significantly promote the growth of Chlorella and achieve the best promotion effect.
[0022] (4) The pharmaceutical product mixture of the present invention will affect the growth of Chlorella. The mixture containing different pharmaceutical pollutants can significantly promote the growth of Chlorella at a low concentration through synergistic effect.
[0023] (5) The present invention utilizes pharmaceutical products to promote the growth of microalgae. On the one hand, it can realize the resource utilization of pharmaceutical by-products, reduce environmental pollution, be environmentally friendly, and reduce treatment costs. On the other hand, it provides a new strategy for large-scale cultivation of microalgae, reduces production costs, improves the market competitiveness of microalgae-related products, and promotes the development of related industries. Attached Figure Description
[0024] Figure 1 The OD of Examples 1-4 and Comparative Example 1 of the present invention 680 A graph showing the change over time;
[0025] Figure 2 The figures show the biomass results of Examples 1-4 and Comparative Example 1 of this invention;
[0026] Figure 3 These are photographs of Embodiments 1-4 and Comparative Example 1 of the present invention;
[0027] Figure 4 The present invention adds the OD of prednisone and dexamethasone, two steroid drugs. 680 A graph showing the change over time;
[0028] Figure 5 The present invention adds the OD values of norfloxacin, enoxacin, fleroxacin, ofloxacin, ciprofloxacin, and lomefloxacin, which are fluoroquinolone antibiotics. 680 A graph showing the change over time;
[0029] Figure 6 To this invention, the OD values of sulfadiazine, sulfamethoxazole, sulfisoxazole, sulfacetyl, and sulfadiazine in sulfonamide antibiotics are added. 680 A graph showing the change over time;
[0030] Figure 7 The invention adds the OD of diclofenac, ibuprofen, and indomethacin, which are nonsteroidal anti-inflammatory drugs. 680 A graph showing the change over time;
[0031] Figure 8 Adding dexamethasone OD to this invention 680 A graph showing the change in concentration over time.
[0032] Figure 9 The OD of the addition of sulfamethoxazole to this invention 680 A graph showing the change in concentration over time.
[0033] Figure 10 Adding norfloxacin to the OD of this invention 680 A graph showing the change in concentration over time.
[0034] Figure 11 Adding indomethacin to the OD of this invention 680 A graph showing the change in concentration over time.
[0035] Figure 12 The OD of the mixed formulation of different pharmaceutical products added to this invention 680 A graph showing the change over time. Detailed Implementation
[0036] The principles and features of the present invention are described below with reference to examples. The examples are only used to explain the present invention and are not intended to limit the scope of the present invention. The parts mentioned in the specific embodiments are parts by weight.
[0037] In the specific implementation method, the Chlorella pyrenoidosa (GY-D26) used was isolated and purified by the Laboratory of the School of Pharmacy, Binzhou Medical University. The specific process is as follows: a Chlorella suspension was obtained by sampling from Jixia Lake, Shandong University of Technology; the Chlorella suspension was serially diluted with sterile water to a concentration of 10. -8 The concentration was determined; 0.1 mL of the diluted solution was dropped onto a BG11 solid medium plate, and the bacterial solution was evenly spread on the plate surface using a sterile spreader to ensure uniform distribution; the inoculated plate was placed upside down in an incubator with a light intensity of 2000 lux, a temperature of 25℃, and a photoperiod of 12h light / 12h dark for one week until a single algal colony grew; a single colony was picked up with a sterile inoculation loop and inoculated onto a new BG11 solid medium plate, and the plate was streaked again, repeated 2-3 times to obtain a purified Chlorella strain; after obtaining the strain, the genome was extracted for species identification; after identification, the obtained strain was Chlorella pyrenoidosa GY-D26.
[0038] All chemical reagents used were commercially available products.
[0039] The absorbance of Chlorella was measured at a UV absorption wavelength of 680 nm using a UV spectrophotometer. Therefore, the growth effect of Chlorella can be judged based on the change in absorbance at 680 nm.
[0040] Example 1
[0041] A method for promoting the growth of Chlorella using pharmaceutical products includes the following steps: activating Chlorella on a BG11 solid plate; using an inoculation loop to collect preserved Chlorella algal solution and streaking it on the BG11 solid plate; picking single algal colonies grown on the plate and inoculating them into liquid BG11 medium for growth; after growth to a deep green color, expanding the culture to obtain activated Chlorella; inoculating the activated Chlorella into liquid BG11 medium; and measuring the initial OD of the Chlorella. 680 The concentration was 0.15, and 20 mg / L of the pharmaceutical steroid prednisone was added at the early stage of Chlorella growth. The mixture was placed in a constant temperature shaker at 80 rpm, with a light intensity of 1000 lux, and cultured at 25℃ for 12 days. The absorbance of the algal solution was measured every 2 days.
[0042] Examples 2-4
[0043] A method for promoting the growth of Chlorella using pharmaceutical products, referring to Example 1, wherein the steroid drug prednisone in Example 1 is replaced sequentially with the fluoroquinolone antibiotic enoxacin, the sulfonamide antibiotic sulfacetamide, and the nonsteroidal anti-inflammatory drug ibuprofen, and the rest are the same as in Example 1.
[0044] Comparative Example 1
[0045] A method for growing Chlorella, referring to Example 1, except that the steroid drug prednisone in Example 1 is removed, and the rest is the same as in Example 1.
[0046] Example 5
[0047] A method for promoting the growth of Chlorella using pharmaceutical products includes the following steps: activating Chlorella on a BG11 solid plate; using an inoculation loop to collect preserved Chlorella algal solution and streaking it on the BG11 solid plate; picking single algal colonies grown on the plate and inoculating them into liquid BG11 medium for growth; after growth to a deep green color, expanding the culture to obtain activated Chlorella; inoculating the activated Chlorella into liquid BG11 medium; and measuring the initial OD of the Chlorella. 680 The concentration was 0.15, and 10 mg / L of the pharmaceutical steroid prednisone was added at the early stage of Chlorella growth. The mixture was placed in a constant temperature shaker at 150 rpm, with a light intensity of 3000 lux, and cultured at 30℃ for 12 days. The absorbance of the algal solution was measured every 2 days.
[0048] Examples 6-20
[0049] A method for promoting the growth of Chlorella using pharmaceutical products, referring to Example 5, wherein the steroid drug prednisone in Example 5 is replaced with dexamethasone in sequence, the fluoroquinolone antibiotics are: norfloxacin, enoxacin, fleroxacin, ofloxacin, ciprofloxacin, and lomefloxacin, the sulfonamide antibiotics are: sulfadiazine, sulfamethoxazole, sulfisoxazole, sulfacetamide, and sulfadiazine, the nonsteroidal anti-inflammatory drugs are: diclofenac, ibuprofen, and indomethacin, and the rest are the same as in Example 5.
[0050] Comparative Example 2
[0051] A method for growing Chlorella, referring to Example 5, except that the steroid drug prednisone in Example 5 is removed, and the rest is the same as in Example 5.
[0052] Example 21
[0053] A method for promoting the growth of Chlorella using pharmaceutical products includes the following steps: activating Chlorella on a BG11 solid plate; using an inoculation loop to collect preserved Chlorella algal solution and streaking it on the BG11 solid plate; picking single algal colonies grown on the plate and inoculating them into liquid BG11 medium for growth; after growth to a deep green color, expanding the culture to obtain activated Chlorella; inoculating the activated Chlorella into liquid BG11 medium; and measuring the initial OD of the Chlorella. 680 The concentration was 0.3, and 5 mg / L of the pharmaceutical steroid dexamethasone was added at the early stage of Chlorella growth. The mixture was placed in a constant temperature shaker at 120 rpm, with a light intensity of 2000 lux, and cultured at 28℃ for 12 days. The absorbance of the algal solution was measured every 2 days.
[0054] Examples 22-24
[0055] A method for promoting the growth of Chlorella using pharmaceutical products, referring to Example 21, wherein the concentrations added in Example 21 are successively replaced with 10 mg / L, 20 mg / L, and 30 mg / L, and the rest are the same as in Example 21.
[0056] Comparative Example 3
[0057] A method for growing Chlorella, referring to Example 21, except that the steroid drug prednisone in Example 21 is removed, and the rest is the same as in Example 21.
[0058] Comparative Examples 4 and 5
[0059] A method for growing Chlorella, referring to Example 21, except that the concentrations added in Example 21 are successively replaced with 0.5 mg / L and 40 mg / L, and the rest are the same as in Example 21.
[0060] Example 25
[0061] A method for promoting the growth of Chlorella using pharmaceutical products, referring to Example 21, except that the steroid drug dexamethasone in Example 21 is replaced with the sulfonamide antibiotic sulfamethoxazole, and the rest is the same as in Example 21.
[0062] Examples 26-28
[0063] A method for promoting the growth of Chlorella using pharmaceutical products, referring to Example 25, wherein the concentrations added in Example 25 are successively replaced with 10 mg / L, 20 mg / L, and 30 mg / L, and the rest are the same as in Example 25.
[0064] Comparative Examples 6 and 7
[0065] A method for growing Chlorella, referring to Example 25, except that the concentrations added in Example 25 are successively replaced with 0.5 mg / L and 40 mg / L, and the rest are the same as in Example 25.
[0066] Example 29
[0067] A method for promoting the growth of Chlorella using pharmaceutical products, referring to Example 21, except that the steroid drug dexamethasone in Example 21 is replaced with the fluoroquinolone antibiotic norfloxacin, and the rest is the same as in Example 21.
[0068] Examples 30-32
[0069] A method for promoting the growth of Chlorella using pharmaceutical products, referring to Example 29, wherein the concentrations added in Example 29 are successively replaced with 10 mg / L, 20 mg / L, and 30 mg / L, and the rest are the same as in Example 29.
[0070] Comparative Examples 8 and 9
[0071] A method for growing Chlorella, referring to Example 29, except that the concentrations added in Example 29 are successively replaced with 0.5 mg / L and 40 mg / L, and the rest are the same as in Example 29.
[0072] Example 33
[0073] A method for promoting the growth of Chlorella using pharmaceutical products, referring to Example 21, except that the steroid drug dexamethasone in Example 21 is replaced with the non-steroidal anti-inflammatory drug indomethacin, and the rest is the same as in Example 21.
[0074] Examples 34-36
[0075] A method for promoting the growth of Chlorella using pharmaceutical products, referring to Example 33, wherein the concentrations added in Example 33 are successively replaced with 10 mg / L, 20 mg / L, and 30 mg / L, and the rest are the same as in Example 33.
[0076] Comparative Examples 10 and 11
[0077] A method for growing Chlorella, referring to Example 33, except that the concentrations added in Example 33 are successively replaced with 0.5 mg / L and 40 mg / L, and the rest are the same as in Example 33.
[0078] Example 37
[0079] A method for promoting the growth of Chlorella using pharmaceutical products, referring to Example 31, replaces the 20 mg / L norfloxacin added in Example 31 with a mixture of four pharmaceutical products at 2.5 mg / L. The mixture includes: 2.5 mg / L dexamethasone, 2.5 mg / L norfloxacin, 2.5 mg / L sulfamethoxazole and 2.5 mg / L indomethacin, with the remainder as in Example 31.
[0080] Example 38
[0081] A method for promoting the growth of Chlorella using pharmaceutical products, referring to Example 31, involves adding 20% of the pharmaceutical product from Example 31. The mg / L norfloxacin was replaced with a mixture of 16 pharmaceutical products at a concentration of 0.1 mg / L, the mixture comprising: 0.1 mg / L prednisone and 0.1 mg / L dexamethasone (steroids); 0.1 mg / L norfloxacin, 0.1 mg / L enoxacin, 0.1 mg / L fleroxacin, 0.1 mg / L ofloxacin, 0.1 mg / L ciprofloxacin and 0.1 mg / L lomefloxacin (fluoroquinolone antibiotics); 0.1 mg / L sulfadiazine, 0.1 mg / L sulfamethoxazole, 0.1 mg / L sulfisoxazole, 0.1 mg / L sulfacetamide and 0.1 mg / L sulfadiazine (sulfonamide antibiotics); 0.1 mg / L diclofenac, 0.1 mg / L ibuprofen and 0.1 mg / L indomethacin (nonsteroidal anti-inflammatory drugs), the remainder being as described in Example 31.
[0082] Comparative Example 12
[0083] A method for growing Chlorella, referring to Example 31, involves replacing the 20 mg / L norfloxacin added in Example 31 with a mixture of 16 pharmaceutical products at a concentration of 0.01 mg / L. This mixture includes: 0.01 mg / L prednisone and 0.01 mg / L dexamethasone (steroid drugs); 0.01 mg / L norfloxacin, 0.01 mg / L enoxacin, 0.01 mg / L fleroxacin, 0.01 mg / L ofloxacin, 0.01 mg / L ciprofloxacin, and 0.01 mg / L of... / L lomefloxacin (fluoroquinolone antibiotic); 0.01 mg / L sulfadiazine, 0.01 mg / L sulfamethoxazole, 0.01 mg / L sulfisoxazole, 0.01 mg / L sulfacetamide and 0.01 mg / L sulfadiazine (sulfonamide antibiotics); 0.01 mg / L diclofenac, 0.01 mg / L ibuprofen and 0.01 mg / L indomethacin (nonsteroidal anti-inflammatory drug), the rest as described in Example 31.
[0084] Results explanation:
[0085] 1. Examples 1-4 investigated the effects of adding different types of pharmaceutical products (steroids, fluoroquinolone antibiotics, sulfonamide antibiotics, or nonsteroidal anti-inflammatory drugs) on the growth of Chlorella. Comparative Example 1, without the addition of pharmaceutical products, served as the control group.
[0086] Biomass on day 8 was calculated based on the OD-dry weight standard curve.
[0087] Chlorella OD 680 Adjust the time to 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, centrifuge and discard the supernatant. Freeze-dry the precipitate, weigh it after freeze-drying, prepare a standard curve, and input the OD of the eighth day into the standard curve to calculate the Chlorella biomass.
[0088] In Examples 1-4 and Comparative Example 1, OD 680 The curve of change over time is as follows Figure 1 As shown, the biomass results on day 8 are as follows: Figure 2 As shown, the photos in each group on the eighth day are as follows: Figure 3 As shown.
[0089] Depend on Figure 1 OD 680 Curve of change over time Figure 2 medium biomass results and Figure 3 The darkening of colors in the photos demonstrates that, compared to Comparative Example 1 without the addition of pharmaceutical products, the addition of low concentrations of steroids, fluoroquinolone antibiotics, sulfonamide antibiotics, and nonsteroidal anti-inflammatory drugs in Examples 1-4 significantly promoted the growth of Chlorella. These pharmaceutical products may promote the growth and biomass accumulation of Chlorella through multiple mechanisms (such as metabolic pathway regulation, enhanced antioxidant capacity, and competitive microbial inhibition).
[0090] 2. Examples 5-20 investigated the effects of adding different types of pharmaceutical products on the growth of Chlorella. Comparative Example 2, without the addition of any pharmaceutical product, served as the control group. The OD values of prednisone and dexamethasone, two of the added steroid drugs, were also studied. 680 The curve of change over time is as follows Figure 4 As shown, the OD values of the fluoroquinolone antibiotics norfloxacin, enoxacin, fleroxacin, ofloxacin, ciprofloxacin, and lomefloxacin were added. 680 The curve of change over time is as follows Figure 5 As shown, the OD values of sulfadiazine, sulfamethoxazole, sulfaisoxazole, sulfacetyl, and sulfadiazine, among other sulfonamide antibiotics, are... 680 The curve of change over time is as follows Figure 6 As shown, the OD values of diclofenac, ibuprofen, and indomethacin, which are nonsteroidal anti-inflammatory drugs, were increased. 680 The curve of change over time is as follows Figure 7 As shown.
[0091] Depend on Figures 4-7 The results demonstrate that, compared to Comparative Example 1 without the addition of pharmaceutical products, the addition of different types of steroid drugs, different types of fluoroquinolone antibiotics, different types of sulfonamide antibiotics, and different types of non-steroidal anti-inflammatory drugs in Examples 5-20 all affected the growth of Chlorella, thus indicating that different types of pharmaceutical products can have a good growth-promoting effect on Chlorella.
[0092] 3. Examples 21-36 investigated the effects of adding different concentrations of the pharmaceutical product on the growth of Chlorella. Comparative Example 3 (without added pharmaceutical product) and Comparative Examples 4-11 (with added pharmaceutical product concentrations of 0.5 mg / L or 40 mg / L) served as control groups. On day 8, the OD of dexamethasone was... 680 The curve of change with concentration is as follows: Figure 8 As shown, the OD of the product with added sulfamethoxazole 680 The curve of change with concentration is as follows: Figure 9 As shown, the OD of norfloxacin added 680 The curve of change with concentration is as follows: Figure 10 As shown, the OD of adding indomethacin 680 The curve of change with concentration is as follows: Figure 11 As shown.
[0093] Depend on Figures 8-11The results demonstrate that, compared to Comparative Example 3 without the addition of the pharmaceutical product and Comparative Examples 4-11 with the addition of the pharmaceutical product at concentrations of 0.5 mg / L or 40 mg / L, the addition of 5-30 mg / L of dexamethasone, sulfamethoxazole, norfloxacin, and indomethacin in Examples 21-36 all promoted the growth of Chlorella; and the optimal promotion effect on the growth of Chlorella was achieved when the concentration of the pharmaceutical product was 20 mg / L.
[0094] 4. Examples 37, 38, and Comparative Example 12 investigated the effects of mixed formulations containing different pharmaceutical products on the growth of Chlorella, comparing them with Comparative Example 3 (without pharmaceutical product) and Example 31 (with 20 mg / L norfloxacin added). In Examples 31, 37, and 38, and Comparative Examples 3 and 12, OD... 680 The curve of change over time is as follows Figure 12 As shown.
[0095] Depend on Figure 12 The results demonstrate that pharmaceutical product mixtures can affect the growth of Chlorella. Mixed formulations containing different pharmaceutical contaminants can significantly promote the growth of Chlorella at lower concentrations through synergistic effects.
[0096] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.
Claims
1. A method for promoting microalgae growth using pharmaceutical products, characterized in that, The process includes the following steps: activating microalgae on a solid plate, inoculating the activated microalgae into a liquid culture medium, adding pharmaceutical products in the early stage of microalgae growth, and culturing in a constant temperature shaker under light. The microalgae is Chlorella vulgaris; The pharmaceutical product is a fluoroquinolone antibiotic; When the fluoroquinolone antibiotic is enoxacin, the concentration added is 10-20 mg / L; When the fluoroquinolone antibiotic is norfloxacin, the concentration added is 0.5~30 mg / L; When the fluoroquinolone antibiotic is fleroxacin or lomefloxacin, the added concentration is 10 mg / L.
2. The method for promoting microalgae growth using pharmaceutical products according to claim 1, characterized in that, The initial OD of the microalgae 680 The concentration of the light source is 0.1~0.3, the shaking speed is 80~150 rpm, the light intensity is 1000~3000 lux, and the culture temperature is 25~30℃.
3. The method for promoting microalgae growth using pharmaceutical products according to claim 1, characterized in that, The solid plate is a BG11 solid plate, and the culture medium is a BG11 culture medium.
4. The method for promoting microalgae growth using pharmaceutical products according to claim 3, characterized in that, The activation includes the following steps: using an inoculation loop to pick up the preserved Chlorella liquid and streak it on a BG11 solid plate; picking up a single algal colony that has grown on the plate and inoculating it into liquid culture medium BG11 for growth; after growing until the color is dark green, it is expanded cultured, and the expanded Chlorella is activated Chlorella.