Kidney-tonifying oyster peptide extraction process

Abstract

The invention discloses a kidney-tonifying oyster peptide extraction process which comprises the following extraction steps: S1, removing shells of fresh oysters, taking meat, washing with running water to remove silt and impurities, pulping the oyster meat by adopting a high-speed tissue crusher, and adding pure water with the volume 3 times that of the oyster meat to prepare homogenate; s2, a stainless steel enzymolysis reaction tank is used, neutral protease and flavourzyme are added, the pH is adjusted to 7.0, the temperature is controlled to be 50 DEG C, and the enzymolysis time is 4 hours; raising the temperature to 85 DEG C and maintaining the temperature for 15 minutes to terminate the enzyme activity; s3, preliminarily removing macromolecular impurities by using 400-mesh filter cloth, and obtaining a clear peptide solution through a tubular centrifuge; s4, adopting a 10kDa hollow fiber ultrafiltration membrane to intercept a target peptide fragment with the molecular weight of 500-1000Da, and then adopting a roll type nanofiltration membrane to concentrate the solution until the solid content is greater than or equal to 25%; s5, carrying out spray drying by using a spray drying unit to obtain faint yellow powder with the water content of less than or equal to 5 percent, and sieving by using a 80-mesh sieve and sub-packaging; and then lycium barbarum polysaccharide or rhizoma polygonati extract is added in proportion to enhance the kidney tonifying effect.

Description

Technical Field

[0001] The invention belongs to the technical field of oyster peptide preparation, in particular to a process for extracting kidney-tonifying oyster peptide. Background Art

[0002] Oyster peptide is a bioactive peptide extracted from oysters. It is rich in various amino acids and trace elements and has high nutritional and medicinal value. Currently, the preparation and extraction methods of oyster peptide mostly use enzymatic hydrolysis technology and membrane separation technology. Membrane separation technology is used to separate oyster peptide from other impurities to obtain a high-purity oyster peptide solution. This can significantly improve the extraction efficiency and purity while ensuring the biological activity of oyster peptide.

[0003] In the concentrated extraction of oyster peptide solutions, drying towers are mainly used for drying. For example, in the prior art, the utility model patent with publication number CN208917112U uses hot air in the drying tower to contact and dry the atomized slurry, thereby increasing the drying speed. However, a relatively high temperature is used for spray drying, which may cause the heat-sensitive components (such as bioactive peptides) in the oyster peptides to be destroyed, thereby reducing their biological activity and medicinal value. In addition, the drying temperature and time cannot be automatically adjusted according to the solution concentration during the drying extraction, resulting in inconsistent oyster peptide content and activity in different batches of products, affecting product quality. Therefore, it is necessary to provide a kidney-tonifying oyster peptide extraction process to solve the problems raised in the above-mentioned background technology. Summary of the Invention

[0004] To achieve the above object, the present invention provides the following technical solution: a process for extracting oyster peptides for tonifying the kidney, comprising the following extraction steps:

[0005] S1. Shell and remove the fresh oysters, rinse with running water to remove sediment and impurities, and use a high-speed tissue crusher to pulp the oyster meat. Add 3 times the volume of pure water to make a homogenate.

[0006] S2. Use a stainless steel enzymatic hydrolysis reaction tank, add neutral protease and flavor protease, adjust the pH to 7.0, control the temperature at 50°C, and perform enzymatic hydrolysis for 4 hours; then raise the temperature to 85°C and maintain for 15 minutes to terminate the enzyme activity;

[0007] S3, using a 400-mesh filter cloth to preliminarily remove macromolecular impurities, and obtaining a clarified peptide solution by a tubular centrifuge;

[0008] S4, using a 10 kDa hollow fiber ultrafiltration membrane to retain the target peptide with a molecular weight cutoff of 500-1000 Da, and then using a spiral nanofiltration membrane to concentrate the solution to a solid content of ≥25%;

[0009] S5. Spray drying is performed using a spray drying unit to obtain a light yellow powder with a moisture content of ≤5%, which is sieved through an 80-mesh sieve and packaged; and wolfberry polysaccharides or polygonatum extract are then added in proportion to enhance the kidney-tonifying effect;

[0010] In S5, the spray drying unit includes a liquid storage tank, an outer barrel, an inner barrel, a graded drying unit, and a cyclone separator; the liquid storage tank is fixed above the outer barrel, a stirring shaft is provided in the liquid storage tank, and a discharge pipe is connected to one side of the lower end surface of the liquid storage tank, and a discharge pump is connected in series to the discharge pipe;

[0011] The inner cylinder is coaxially rotatably arranged in the outer cylinder, and a plurality of evaporation chambers are distributed circumferentially in the inner cylinder, and microporous liquid drainage seats are arranged above the interior of each evaporation chamber;

[0012] The graded drying unit is fixed in the outer cylinder and connected below the inner cylinder, and the graded drying unit is in sealed communication with each of the evaporation chambers;

[0013] A discharge pipe is vertically connected to the middle portion of the lower end surface of the outer barrel. The discharge pipe passes through the outer barrel and is connected to the cyclone separator.

[0014] Furthermore, preferably, a sealing seat is coaxially mounted on the upper end of the inner cylinder, and a plurality of inner channel tubes corresponding to the evaporation chambers are distributed in the sealing seat, and the lower parts of the inner channel tubes are connected to the microporous liquid drainage seat through drainage channels;

[0015] A liquid guide valve is fixed on the upper part of the inner part of the outer tank, the upper part of the liquid guide valve is connected to the liquid discharge pipe, the liquid guide valve is rotatably connected to the sealing seat, and a bypass channel is opened on one side of the liquid guide valve, and the bypass channel is connected to the inner channel pipe.

[0016] Furthermore, as a preferred embodiment, a concentration detection probe is installed on the liquid discharge pipe; a hot air discharge cylinder is fixed in the middle of the outer cylinder, an exhaust pipe is installed below the hot air discharge cylinder, and a plurality of microfiltration holes are opened on the side wall of the hot air discharge cylinder, and a plurality of air holes are opened at corresponding positions on the side wall of the evaporation chamber;

[0017] A driving part is installed on the outer barrel, and an output end of the driving part is connected with the inner barrel for transmission through gear meshing.

[0018] Furthermore, preferably, the lower inner wall of the evaporation chamber is configured as a slope structure, and a discharge port is provided on the inner wall near the bottom of the slope, a connecting hole is provided on the middle inner wall of the outer barrel, the upper end of the discharge pipe is sealed and connected to the connecting hole, and each of the discharge ports is connected to the connecting hole as the inner barrel rotates.

[0019] Furthermore, as a preference, the graded drying unit comprises an air ring seat, which is fixed concentrically in the outer cylinder, and a plurality of air supply cavities are distributed on the inner circumference of the air ring seat, and the lower parts of the air supply cavities are connected to hot air seats, and the air supply cavities are arranged in sequence according to the heating level;

[0020] An air flow disk is installed above the air ring seat, and a plurality of air holes are opened on the air flow disk at each air delivery cavity;

[0021] A plurality of heat-conducting columns are vertically fixed in the evaporation chamber, and the heat-conducting columns are arranged in a one-to-one correspondence with the air holes.

[0022] Furthermore, preferably, the high-temperature heat flow transported from each of the hot air seats passes through the heat-conducting columns and enters the evaporation chamber, and the temperatures of the high-temperature heat flow in the hot air seats are different and rise in a gradient.

[0023] Furthermore, as a preference, a tracheal sleeve is fixed to the lower end of the heat-conducting column, a rubber ring is provided on the outer surface of the tracheal sleeve, and the heat-conducting column is sealed and connected to the air hole through the rubber ring;

[0024] A shaft tube is slidably connected in the heat-conducting column, a plurality of micro-holes are opened on the side wall of the shaft tube, and a diversion hole is provided on the outer wall of the heat-conducting column;

[0025] A valve tube is inherently provided below the shaft tube. The valve tube is in sealing and sliding cooperation with the tracheal sleeve, and a side hole is provided on the side wall of the valve tube.

[0026] Furthermore, preferably, a shaft column is rotatably connected to the upper part of the heat-conducting column, an assembly gap is provided between the shaft column and the heat-conducting column, an inner spring is connected between the shaft column and the shaft tube, and the upper end of the shaft column is in contact with the heat-conducting column, and the contact surface thereof is configured as a toothed disc structure;

[0027] An axle pin is fixed to the inner wall of the axle tube, a guide groove is provided in the axle column, and the axle pin is slidably connected to the guide groove.

[0028] Furthermore, preferably, each of the hot air seats delivers high-temperature heat flow in a pulsed manner or a steady-pressure manner; and the elastic strength of the inner springs in each heat-conducting column in the evaporation chamber is different.

[0029] Compared with the prior art, the present invention has the following beneficial effects:

[0030] The prefabricated oyster peptide solution in the present invention can be continuously and evenly stirred in the liquid storage tank, and when the liquid guide valve is connected to the inner channel tube in the sealing seat, the oyster peptide solution can enter the microporous drainage seat through the drainage channel, and be sprayed out by the microporous drainage seat to form a mist liquid and diffusely distributed in the evaporation chamber, thereby realizing the evaporation extraction of the oyster peptide solution. Here, the evaporation chamber can be connected with the air supply chambers of different heat supply levels in the graded drying unit as the inner cylinder rotates, so that the oyster peptide mist in the evaporation chamber can achieve a multi-stage evaporation drying effect, thereby being able to dynamically adjust the evaporation drying intensity based on the change in the concentration of the oyster peptide solution in the evaporation chamber. On the one hand, it avoids the destruction of heat-sensitive components by continuous high temperature, and maximizes the retention of the biological activity and medicinal value of the oyster peptide. On the other hand, it helps to maintain the stability of the bioactive components in the oyster peptide, avoids over-drying or insufficient drying, and improves the quality consistency of the final product. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 is a process flow chart of the present invention;

[0032] Figure 2 It is a schematic diagram of the overall structure of the present invention;

[0033] Figure 3 for Figure 2 A schematic diagram of the structure at center A;

[0034] Figure 4 is a cross-sectional view of the evaporation chamber in the present invention;

[0035] Figure 5 Schematic diagram of the structure of the graded drying unit in the present invention;

[0036] Figure 6 Schematic diagram of the structure of the air ring seat and the air flow disk in the present invention;

[0037] Figure 7 Schematic diagram of the structure of the heat-conducting column in the present invention;

[0038] Figure 8 It is a structural schematic diagram of the central axis column of the present invention;

[0039] In the figure: 1. Liquid storage tank; 11. Drain pipe; 12. Drain pump; 13. Cyclone separator; 2. Outer tank; 21. Discharge pipe; 22. Liquid guide valve; 23. Bypass channel; 24. Hot air discharge cylinder; 25. Exhaust pipe; 3. Inner cylinder; 31. Evaporation chamber; 32. Microporous drainage seat; 33. Sealing seat; 34. Inner channel tube; 35. Drain channel; 36. Air hole; 37. Delivery and discharge port; 4. Grading drying unit; 41. Air ring seat; 42. Air supply chamber; 43. Air flow disk; 44. Air hole; 45. Hot air seat; 5. Heat conducting column; 51. Air pipe sleeve; 52. Rubber ring; 53. Shaft tube; 54. Micropore; 55. Valve tube; 56. Bypass hole; 57. Shaft column; 58. Shaft pin. DETAILED DESCRIPTION

[0040] See also Figures 1-8 In an embodiment of the present invention, a kidney-tonifying oyster peptide extraction process comprises the following extraction steps:

[0041] S1. Fresh oysters were shelled and the meat was removed. The meat was rinsed with running water to remove sediment and impurities. The oyster meat was pulped (particle size ≤ 0.5 mm) using a high-speed tissue crusher and then slurried with 3 times the volume of pure water.

[0042] S2, using a stainless steel enzymatic reaction tank (equipped with a pH / temperature automatic control system that can accurately adjust pH and temperature), add neutral protease (enzyme activity 5×10 4 U / g, dosage 1.5%) and flavor protease (enzyme activity 3×10 4 U / g, dosage 0.8%), adjust pH to 7.0, control temperature at 50°C, enzymatic hydrolysis time 4 hours; then raise temperature to 85°C and maintain for 15 minutes to terminate enzyme activity;

[0043] S3. Use 400 mesh filter cloth to preliminarily remove macromolecular impurities, and obtain a clarified peptide solution by tubular centrifuge (parameters: 12000 rpm\15 min);

[0044] S4. Use a 10 kDa hollow fiber ultrafiltration membrane to retain the target peptide with a molecular weight cutoff of 500-1000 Da, and then use a spiral nanofiltration membrane (molecular weight cutoff of 200 Da) to concentrate the solution to a solid content of ≥25%;

[0045] S5. Use a spray drying unit to spray dry the powder to obtain a light yellow powder with a moisture content of ≤5%, and sieve through an 80-mesh sieve for packaging; then add wolfberry polysaccharide (3%) or polygonatum extract (2%) in proportion to enhance the kidney-tonifying effect.

[0046] In the S5, the spray drying unit includes a liquid storage tank 1, an outer barrel 2, an inner barrel 3, a graded drying unit 4 and a cyclone separator 13; the liquid storage tank 1 is fixed above the outer barrel 2, a stirring shaft is provided in the liquid storage tank 1, and a discharge pipe 11 is connected to one side of the lower end surface thereof, and a discharge pump 12 is connected in series to the discharge pipe 11; peptide substances are extracted from oysters after enzymatic hydrolysis, and the clarified liquid is extracted after filtering and impurity removal and concentrated to form a prefabricated oyster peptide solution, which can be stored in the liquid storage tank 1;

[0047] The inner cylinder 3 is coaxially rotatably disposed in the outer cylinder 2. A plurality of evaporation chambers 31 are circumferentially distributed in the inner cylinder 3. A microporous liquid discharge seat 32 is disposed above each of the evaporation chambers 31. The microporous liquid discharge seat 32 is used to diffuse and spray the oyster peptide solution into a mist. The viscosity of the oyster peptide solution must be between 10 and 50 cP to ensure that the solution can smoothly pass through the nozzle of the microporous liquid discharge seat 32 to form uniform droplets.

[0048] The graded drying unit 4 is fixed in the outer cylinder 2 and connected to the bottom of the inner cylinder 3. The graded drying unit 4 is in sealed communication with each of the evaporation chambers 31.

[0049] A discharge pipe 21 is vertically connected to the middle of the lower end surface of the outer barrel 2. The discharge pipe 21 passes through the outer barrel 2 and is connected to the cyclone separator 13. Therefore, the dried oyster peptide powder enters the cyclone separator 13 through the discharge pipe 21, and is finally collected and discharged by the cyclone separator 13.

[0050] In this embodiment, a sealing seat 33 is coaxially mounted on the upper end of the inner cylinder 3. A plurality of inner channel tubes 34 corresponding to the evaporation chamber 31 are distributed in the sealing seat 33. The lower portions of the inner channel tubes 34 are connected to the microporous liquid drainage seat 32 via drainage channels 35.

[0051] A liquid guide valve 22 is fixed on the upper part of the inner part of the outer canister 2. The upper part of the liquid guide valve 22 is connected to the liquid discharge pipe 11. The liquid guide valve 22 is rotatably connected to the sealing seat 33, and a bypass channel 23 is opened on one side of the liquid guide valve 22. The bypass channel 23 is connected to the inner channel tube 34. When the inner canister rotates, the bypass channel 23 on the liquid guide valve 22 can connect with each inner channel tube 34 one by one, thereby transporting the oyster peptide solution to each evaporation chamber 31 and spraying it out in atomization from the microporous liquid discharge seat 32 in the evaporation chamber 31.

[0052] As a preferred embodiment, a concentration detection probe is installed on the liquid discharge pipe 11; a hot air discharge cylinder 24 is fixed to the middle of the outer cylinder 2, and an exhaust pipe 25 is installed below the hot air discharge cylinder 24. A plurality of microfiltration holes are opened on the side wall of the hot air discharge cylinder 24, and a plurality of air holes 36 are opened at corresponding positions on the side wall of the evaporation chamber 31; when the microfiltration holes and the air holes 36 are connected to each other, the high-temperature heat flow in the evaporation chamber 31 can be discharged through the microfiltration holes, thereby realizing the flow and discharge of the high-temperature heat flow in the evaporation chamber 31;

[0053] A driving part (not shown in the figure) is installed on the outer barrel 2, and the output end of the driving part is connected to the inner barrel 3 for transmission through gear meshing.

[0054] In this embodiment, the lower inner wall of the evaporation chamber 31 is set to a slope structure, and a discharge port 37 is opened on its inner wall near the bottom of the slope. A connecting hole is opened on the middle inner wall of the outer barrel 2, and the upper end of the discharge pipe 21 is sealed and connected to the connecting hole. Each of the discharge ports 37 rotates with the inner barrel 3 and is connected to the connecting hole. The evaporated and dried oyster peptide powder can be accumulated at the discharge port 37 position through the slope structure of the inner wall of the evaporation chamber 31. When the discharge port 37 is connected to the connecting hole, the oyster peptide powder enters the discharge pipe 21 through the connecting hole.

[0055] In this embodiment, the graded drying unit 4 includes an air ring seat 41, which is fixed concentrically in the outer cylinder 2. A plurality of air supply cavities 42 are distributed on the inner circumference of the air ring seat 41. The lower parts of the air supply cavities 42 are connected to hot air seats 45. The air supply cavities 42 are arranged in sequence according to the heating level.

[0056] An air flow disk 43 is installed above the air ring seat 41, and a plurality of air holes 44 are opened on the air flow disk 43 at each air delivery cavity 42;

[0057] A plurality of heat-conducting columns 5 are vertically fixed in the evaporation chamber 31, and the heat-conducting columns 5 are arranged in a one-to-one correspondence with each of the air holes 44. The plurality of heat-conducting columns 5 in the evaporation chamber 31 increase the contact area between the mist-like oyster peptide solution and the heat source, thereby accelerating the drying speed. At the same time, it is convenient for the oyster peptide solution to mainly adhere to the heat-conducting columns 5, while reducing the probability of its adherence to the inner wall of the evaporation chamber 31, which can effectively avoid scaling and adhesion problems on the inner wall of the evaporation chamber 31.

[0058] In this embodiment, the high-temperature heat flow transported in each of the hot air seats 45 passes through the heat-conducting column 5 and enters the evaporation chamber 31, and the temperature of the high-temperature heat flow in the hot air seat 45 is different and rises in a gradient. Therefore, when the evaporation chamber 31 rotates with the inner cylinder 3, the evaporation chamber 31 can be connected one by one with the air supply chamber 42 of different heat supply levels, thereby realizing multi-stage evaporation and drying of the oyster peptide solution. The concentration detection probe on the liquid pipe 11 can monitor the concentration change of the oyster peptide solution during delivery and spraying in real time, so as to adjust the concentration of the oyster peptide solution based on the concentration of the oyster peptide solution in the evaporation chamber 31. The evaporation drying intensity can be adjusted dynamically. For example, the initial concentration of the oyster peptide solution is 20%. At this time, the water content in the solution is relatively high. A lower hot air temperature (such as 80°C) and a slower air flow rate (such as 5m / s) are used in the initial drying. In the secondary drying, the hot air temperature can be increased by about 10°C and the air flow rate by 1-2m / s. The same is true for the third, fourth, and fifth stage drying. The gradient size between the drying links at each level can be adjusted to maximize the working capacity of the equipment, shorten the overall drying time, and improve production efficiency.

[0059] As a preferred embodiment, a tracheal sleeve 51 is fixed to the lower end of the heat-conducting column 5, and a rubber ring 52 is provided on the outer cover of the tracheal sleeve 51. The heat-conducting column 5 is sealed and docked with the air hole 44 through the rubber ring 52;

[0060] A shaft tube 53 is slidably connected to the heat-conducting column 5, and a plurality of micro-holes 54 are opened on the side wall of the shaft tube 53, and a diversion hole is provided on the outer wall of the heat-conducting column 5;

[0061] A valve tube 55 is inherently provided below the shaft tube 53. The valve tube 55 is in sealed sliding cooperation with the tracheal sleeve 51, and a side hole 56 is provided on the side wall of the valve tube 55. High-temperature heat flow enters the tracheal sleeve 51 and the air pressure pushes the valve tube 55 to slide vertically, thereby entering the shaft tube 53 through the side hole 56 on the valve tube 55. At this time, the micropores 54 on the shaft tube 53 are connected to the diversion hole, and the high-temperature heat flow is discharged through the diversion hole.

[0062] In this embodiment, a shaft column 57 is rotatably connected to the upper inner portion of the heat-conducting column 5, and an assembly gap is provided between the shaft column 57 and the heat-conducting column 5, and an inner spring is connected between the shaft column 57 and the shaft tube 53, and the upper end of the shaft column 57 is in contact with the heat-conducting column 5, and its contact surface is set as a toothed disc structure; when the shaft column 57 rotates relative to the heat-conducting column 5, it can realize the vibration of the end of the heat-conducting column 5 through the toothed disc contact surface with the heat-conducting column 5, so that the oyster peptide powder attached to the surface of the heat-conducting column 5 is quickly detached, that is, when the shaft column 57 and the heat-conducting column 5 are relatively deflected, the tooth peaks of the toothed disc structure thereof periodically contact and stagger with each other, thereby forming an instantaneous release of energy and causing a short vibration effect; specifically, when the tooth peaks of the two toothed disc structures contact, the shaft column 57 and the heat-conducting column 5 form a maximum gap difference, and when the tooth peaks stagger, the gap difference disappears, thereby causing the end of the heat-conducting column 5 to produce a short vibration effect, with a small vibration amplitude but a high frequency;

[0063] The inner wall of the shaft tube 53 is fixed with a shaft pin 58, and a guide groove is provided in the shaft column 57, and the shaft pin 58 is slidably connected to the guide groove; specifically, when a high-temperature heat flow is transported in the tracheal sleeve 51, the end of the valve tube 55 can slide upward and separate from the tracheal sleeve 51. At this time, the shaft tube 53 slides upward synchronously with the valve tube 55 (the shaft tube 53 does not rotate), and the high-temperature heat flow can enter the shaft tube 53 through the bypass hole 56. The guide groove is set to an arc structure, which can cause the shaft column 57 to deflect when the shaft pin 58 on the shaft tube 53 slides upward; and when the shaft tube 53 slides downward, the shaft column 57 rotates in the opposite direction. Therefore, when the high-temperature heat flow enters the heat-conducting column 5 in a pulsed manner, the valve tube 55 in the tracheal sleeve 51 can push the shaft tube 53 to slide while continuously sliding up and down. At this time, the shaft pin 58 on the shaft tube 53 realizes a rapid deflection of the shaft column 57 through the sliding action with the guide groove, thereby realizing the shaking of the heat-conducting column 5.

[0064] In this embodiment, each of the hot air seats 45 delivers high-temperature heat flow in a pulsed manner or a steady-pressure manner; the elastic strength of the inner springs in each of the heat-conducting columns 5 in the evaporation chamber 31 is different, so that the corresponding heat-conducting columns 5 can be opened to supply air by precisely controlling the pressure of the high-temperature heat flow. For example, when the air pressure is low, only the heat-conducting column 5 with the smallest elastic force will open; as the air pressure gradually increases, the heat-conducting columns 5 with larger elastic force will also open in turn, which allows the system to accurately adjust the air supply according to actual needs, avoid delivering too much high-temperature gas at one time, and prevent the temperature or pressure in the evaporation chamber 31 from being too high.

[0065] Specifically, the above-mentioned spray drying extraction comprises the following steps:

[0066] Step 1: The pre-made oyster peptide solution is delivered to the liquid storage tank 1, and the stirring shaft in the liquid storage tank 1 continuously rotates and stirs;

[0067] Step 2: The stirred prefabricated oyster peptide solution is pumped to the liquid guide valve 22 through the drainage pump 12 on the drainage pipe 11, and enters the corresponding inner channel tube 34 through the bypass channel 23 on the drainage valve 22. Finally, the oyster peptide solution is evenly sprayed into a fine mist through the microporous drainage seat 32 below, thereby being evenly distributed in the evaporation chamber 31;

[0068] Step 3: Adjust the working intensity of the graded drying unit 4 according to the overall concentration of the oyster peptide solution in the evaporation chamber 31; wherein, the graded drying unit 4 at the lower part of the outer barrel 2 transmits high-temperature heat flow through the first-level air supply cavity 42 in the air ring seat 41. At this time, the high-temperature heat flow passes through the heat-conducting columns 5 at the lower part of the evaporation chamber 31 and enters the evaporation chamber 31, thereby performing primary evaporation of the water in the mist liquid oyster peptide solution; the multiple heat-conducting columns 5 in the evaporation chamber 31 increase the contact area between the mist liquid oyster peptide solution and the heat source, accelerate the drying speed, and at the same time facilitate the oyster peptide solution to mainly adhere to the heat-conducting columns 5, while reducing the probability of it adhering to the inner wall of the evaporation chamber 31, which can effectively avoid scaling and adhesion problems on the inner wall of the evaporation chamber 31, optimize the drying process, improve product quality, simplify the operation process, and reduce the maintenance cost and cleaning frequency of the equipment.

[0069] Step 4: The driving unit drives the inner cylinder 3 to perform directional deflection, and the evaporation chamber 31 is connected to the secondary air supply chamber 42 in the air ring seat 41. At this time, on the one hand, the liquid guide valve 22 delivers the oyster peptide solution to the microporous liquid discharge seat 32 in the new evaporation chamber 31 through the bypass channel 23, and on the other hand, the secondary air supply chamber 42 delivers high-temperature heat flow to the evaporation chamber 31 again;

[0070] Step 5: Repeat step 4 to allow the mist-like oyster peptide solution in the evaporation chamber 31 to evaporate through multiple stages of temperature rise, ensuring that the water in the droplets is completely evaporated to form a dry powder. Part of the oyster peptide solution will form dry powder and accumulate at the bottom of the slope of the evaporation chamber 31, and the other part will remain on the surface of the heat-conducting column 5. At this time, the hot air seat 45 pulses high-temperature heat flow, causing the shaft tube 53 in the heat-conducting column 5 to continuously slide up and down, so that the shaft column 57 rotates forward and backward, thereby achieving micro-vibration of the end of the heat-conducting column 5 under the contact action of the toothed disc structure, so that the dry powder can be quickly separated;

[0071] Step 6: The dried oyster peptide powder is discharged through the discharge pipe 21 in the outer barrel 2, and the dried oyster peptide powder is captured and separated by the cyclone separator 13 to ensure effective collection of the powder.

[0072] The above is only a preferred specific embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any technician familiar with this technical field, within the technical scope disclosed by the present invention, who makes equivalent replacements or changes based on the technical solutions and inventive concepts of the present invention, should be covered by the scope of protection of the present invention.

Claims

1. A kidney-tonifying oyster peptide extraction process, characterized in that: The extraction steps include: S1. Shell and remove the fresh oysters, rinse with running water to remove sediment and impurities, and use a high-speed tissue crusher to pulp the oyster meat. Add 3 times the volume of pure water to make a homogenate. S2. Use a stainless steel enzymatic hydrolysis reaction tank, add neutral protease and flavor protease, adjust the pH to 7.0, control the temperature at 50°C, and perform enzymatic hydrolysis for 4 hours; then raise the temperature to 85°C and maintain for 15 minutes to terminate the enzyme activity; S3, using a 400-mesh filter cloth to preliminarily remove macromolecular impurities, and obtaining a clarified peptide solution by a tubular centrifuge; S4, using a 10 kDa hollow fiber ultrafiltration membrane to retain the target peptide with a molecular weight cutoff of 500-1000 Da, and then using a spiral nanofiltration membrane to concentrate the solution to a solid content of ≥25%; S5. Use a spray drying unit to perform spray drying to obtain a light yellow powder with a moisture content of ≤5%, and sieve through an 80-mesh sieve for packaging; and then add wolfberry polysaccharide or polygonatum extract in proportion to enhance the kidney-tonifying effect.

2. The process for extracting oyster peptides for invigorating the kidney according to claim 1, wherein: In the S5, the spray drying unit comprises a liquid storage tank (1), an outer barrel (2), an inner barrel (3), a graded drying unit (4), and a cyclone separator (13); wherein the liquid storage tank (1) is fixed above the outer barrel (2), a stirring shaft is provided in the liquid storage tank (1), and a liquid discharge pipe (11) is connected to one side of the lower end surface thereof, and a liquid discharge pump (12) is connected in series to the liquid discharge pipe (11); The inner cylinder (3) is coaxially rotatably disposed in the outer cylinder (2), and a plurality of evaporation chambers (31) are circumferentially distributed in the inner cylinder (3). Microporous liquid drainage seats (32) are disposed above the interiors of the evaporation chambers (31); The graded drying unit (4) is fixed in the outer cylinder (2) and connected below the inner cylinder (3), and the graded drying unit (4) is in sealed communication with each of the evaporation chambers (31); A discharge pipe (21) is vertically connected to the middle of the lower end surface of the outer barrel (2), and the discharge pipe (21) passes through the outer barrel (2) and is connected to the cyclone separator (13).

3. The process for extracting oyster peptides for invigorating the kidney according to claim 2, wherein: A sealing seat (33) is coaxially mounted on the upper end of the inner cylinder (3), and a plurality of inner channel tubes (34) corresponding to the evaporation chamber (31) are distributed in the sealing seat (33), and the lower parts of the inner channel tubes (34) are connected to the microporous liquid drainage seat (32) through drainage channels (35); A liquid guide valve (22) is fixed on the upper part of the inner portion of the outer canister (2). The upper part of the liquid guide valve (22) is connected to the liquid discharge pipe (11). The liquid guide valve (22) is rotatably connected to the sealing seat (33). A bypass channel (23) is provided on one side of the liquid guide valve (22). The bypass channel (23) is connected to the inner channel pipe (34).

4. The process for extracting oyster peptides for invigorating the kidney according to claim 3, wherein: A concentration detection probe is installed on the liquid discharge pipe (11); a hot air discharge cylinder (24) is fixed in the middle of the outer cylinder (2); an exhaust pipe (25) is installed below the hot air discharge cylinder (24); a plurality of microfiltration holes are opened on the side wall of the hot air discharge cylinder (24); and a plurality of air holes (36) are opened at corresponding positions on the side wall of the evaporation chamber (31); A driving part is installed on the outer barrel (2), and the output end of the driving part is connected to the inner barrel (3) for transmission through gear meshing.

5. The process for extracting oyster peptides for invigorating the kidney according to claim 2, characterized in that: The lower inner wall of the evaporation chamber (31) is configured as a slope structure, and a delivery port (37) is provided on the inner wall near the bottom of the slope. A connecting hole is provided on the middle inner wall of the outer barrel (2). The upper end of the discharge pipe (21) is sealed and connected to the connecting hole. Each of the delivery ports (37) rotates with the inner barrel (3) and is connected to the connecting hole.

6. The process for extracting kidney-tonifying oyster peptides according to claim 3, characterized in that: The graded drying unit (4) includes an air ring seat (41) fixed concentrically in the outer cylinder (2), a plurality of air delivery cavities (42) are distributed on the inner circumference of the air ring seat (41), and a hot air seat (45) is connected below each of the air delivery cavities (42). The air delivery cavities (42) are arranged and divided in sequence according to the heating level. An air flow disk (43) is installed above the air ring seat (41), and a plurality of air holes (44) are provided on the air flow disk (43) at each air delivery cavity (42); A plurality of heat-conducting columns (5) are vertically fixed in the evaporation chamber (31), and the heat-conducting columns (5) are arranged in a one-to-one correspondence with each of the air holes (44).

7. The process for extracting oyster peptides for invigorating the kidney according to claim 6, characterized in that: The high-temperature heat flow transported in each of the hot air seats (45) passes through the heat-conducting column (5) and enters the evaporation chamber (31), and the temperatures of the high-temperature heat flow in the hot air seats (45) are different and rise in a gradient.

8. The process for extracting kidney-tonifying oyster peptides according to claim 6, characterized in that: A tracheal sleeve (51) is fixed to the lower end of the heat-conducting column (5), and a rubber ring (52) is provided on the outer cover of the tracheal sleeve (51). The heat-conducting column (5) is sealed and docked with the air hole (44) through the rubber ring (52); A shaft tube (53) is slidably connected inside the heat-conducting column (5), a plurality of micropores (54) are provided on the side wall of the shaft tube (53), and a diversion hole is provided on the outer wall of the heat-conducting column (5); A valve tube (55) is provided below the shaft tube (53). The valve tube (55) is in sealing and sliding cooperation with the tracheal sleeve (51), and a side hole (56) is provided on the side wall of the valve tube (55).

9. The process for extracting kidney-tonifying oyster peptides according to claim 8, characterized in that: A shaft column (57) is rotatably connected to the upper part of the interior of the heat-conducting column (5), an assembly gap is provided between the shaft column (57) and the heat-conducting column (5), an inner spring is connected between the shaft column (57) and the shaft tube (53), and the upper end of the shaft column (57) is in contact with the heat-conducting column (5), and the contact surface thereof is provided as a toothed disc structure; An axle pin (58) is fixed to the inner wall of the axle tube (53), a guide groove is provided in the axle column (57), and the axle pin (58) is slidably connected to the guide groove.

10. The process for extracting kidney-tonifying oyster peptides according to claim 9, characterized in that: Each of the hot air seats (45) delivers high-temperature heat flow in a pulsed manner or a steady-pressure manner; and the elastic strength of the inner springs in each of the heat-conducting columns (5) in the evaporation chamber (31) is different.

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