Blood culture bottle capable of rapidly and accurately detecting

Abstract

The invention provides a blood culture bottle capable of rapidly and accurately detecting, and belongs to the technical field of blood culture bottle preparation. A culture solution in the blood culture bottle comprises brain heart immersion liquid, SPS, activated carbon and hemolysin. The hemolysin is added into the blood culture bottle, so that the bacteria and the blood cells are conveniently distinguished, the blood cells are prevented from interfering bacteria counting to influence a detection result, and finally, the negative and positive properties are determined through Coulter counting. Therefore, the problems that a traditional blood culture bottle needs long-time culture and blood cells are not completely adsorbed by resin, so that a detection result is possibly influenced can be avoided, and the culture time can be compressed to 2 hours by determining the positive and negative properties through Coulter counting without a traditional positive reporting mode.

Description

Technical Field

[0001] The present invention relates to the technical field of blood culture bottle preparation, and in particular to a blood culture bottle capable of rapid and accurate detection. Background Art

[0002] Blood culture bottles are a common clinical tool for diagnosing bacterial infections. By culturing a patient's blood sample in a blood culture bottle, the pathogenic bacteria causing the infection can be quickly identified, including the bacterial species and antibiotic susceptibility. This is crucial for doctors to select the appropriate antibiotic for treatment, effectively improving treatment outcomes and reducing patient mortality.

[0003] In addition to bacterial infections, blood culture bottles are also used to detect fungal infections. Although fungal infections grow relatively slowly and require longer to culture, blood culture bottles still provide reliable test results. This is also of great significance for the diagnosis and treatment of fungal infections.

[0004] In medical laboratories, researchers can culture blood samples from patients in blood culture bottles containing different antibiotics to determine the bacterial sensitivity to these antibiotics. This method can help doctors understand a patient's antibiotic resistance profile and select appropriate antibiotics for treatment, avoiding unnecessary drug use and the development of drug resistance.

[0005] Some pathogens may multiply slowly in the patient's body and may present latent symptoms, making them difficult to detect through routine examinations. Blood culture bottles can help doctors identify potential latent infections by continuously monitoring the culture status, allowing doctors to take timely treatment measures to prevent the disease from worsening.

[0006] Blood culture bottles are also commonly used to detect infections during surgical procedures, drainage, and stomas. By collecting blood samples from patients for culture, postoperative infections can be detected and diagnosed promptly, providing doctors with a basis for timely diagnosis and treatment, helping to reduce postoperative complications and improve surgical success rates.

[0007] Cancer patients may experience a decrease in immunity after radiotherapy or chemotherapy, making them more susceptible to infection. Blood culture bottles can help doctors detect these infections in a timely manner so that appropriate treatment measures can be taken to protect the patient's life safety.

[0008] In addition, blood culture bottles can also be used to monitor hospital infections, assess antibiotic sensitivity, and study microbial growth characteristics. These application scenarios demonstrate the importance and wide application value of blood culture bottles in clinical medicine.

[0009] In summary, blood culture bottles have a wide range of applications and significant value in clinical medicine. They are not only used for the diagnosis and treatment of bacterial and fungal infections, but can also be used for antibiotic resistance testing, identifying occult infections, and detecting surgical and postoperative infections. Therefore, blood culture bottles are an indispensable tool in clinical microbiology laboratories.

[0010] However, in the prior art, the culture time in traditional culture bottles is relatively long, and incomplete adsorption of blood cells by the resin may affect the test results. In addition, the base membrane preparation process is relatively complicated.

[0011] Based on this, providing a blood culture bottle that can be quickly and accurately detected has important practical significance. Summary of the Invention

[0012] The purpose of the present invention is to provide a blood culture bottle that can be used for rapid and accurate testing, aiming to solve the technical problems in the prior art that traditional culture bottles have a long culture time, blood cells are not completely adsorbed by the resin, which may affect the test results, and the bottom membrane preparation process is relatively complex.

[0013] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0014] The present invention provides a blood culture bottle capable of rapid and accurate detection, wherein the culture fluid in the blood culture bottle comprises brain heart infusion, SPS, activated carbon and hemolysin.

[0015] Furthermore, the added amounts of the brain heart infusion and SPS are 2.4g-5.6g and 0.5g-1.18g respectively.

[0016] Furthermore, the activated carbon is specifically an activated carbon fiber filter, and the diameter of the activated carbon fiber filter is 3.4 cm to 3.6 cm; the hemolysin is specifically saponin, and the added amount of the saponin is 0.018 g to 0.042 g.

[0017] Furthermore, the culture solution also includes tryptone soy broth, tryptone, yeast powder, yeast nitrogen source, glucose, sucrose, sodium hydroxide, hemin chloride, vitamin K and pure water.

[0018] Furthermore, the added amounts of the tryptone soy broth, tryptone, yeast powder, yeast nitrogen source, glucose, sucrose, sodium hydroxide, hemin chloride, vitamin K, and pure water are 10.8g~25.2g, 4.8g~11.2g, 0.15g~0.35g, 0.15g~0.35g, 1.2g~2.8g, 2.1g~4.9g, 0.48g~1.12g, 0.003g~0.007g, 0.0003g~0.0007g, and 1L, respectively.

[0019] Furthermore, the concentration of the saponin is 0.05% to 0.1%.

[0020] Furthermore, the pH of the culture solution is 7.0-7.4.

[0021] The present invention also provides a method for preparing a blood culture bottle capable of rapid and accurate detection as described in the above technical solution, the steps of the preparation method are:

[0022] Mixing the raw materials in sequence to obtain a mixture;

[0023] The mixture was filtered through a microporous membrane and an activated carbon fiber filter was added to the culture medium, 30 mL of which was added to a PC bottle, and the bottle was sealed;

[0024] Then, the blood culture bottle capable of rapid and accurate detection is obtained after high pressure treatment at 121° C. for 15 to 20 minutes.

[0025] Furthermore, the tryptone soy broth, tryptone, yeast powder, yeast nitrogen source, glucose, sucrose, sodium hydroxide, hemin chloride, vitamin K, pure water and saponin are mixed.

[0026] Furthermore, the microporous filter membrane is a microporous filter membrane with a diameter of 0.22 mm to 0.25 mm.

[0027] Compared with the prior art, the technical solution of the present invention has the following beneficial effects:

[0028] Coulter counting typically requires digestion and suspension of cells in culture bottles. High protein in the culture medium (such as serum) can interfere with the Coulter count, leading to well blockage or signal noise. The formulation of the culture bottle indirectly determines the quality of the Coulter count sample and the reliability of the results by affecting the cell growth state, suspension composition, and physical properties. The present invention adds hemolysin to the blood culture bottle, which facilitates the differentiation of bacteria and blood cells, preventing blood cells from interfering with bacterial counts and affecting test results. Determining positive and negative results through Coulter counting can circumvent the problems of traditional blood culture bottles requiring long incubation times and the problem of not all blood cells being adsorbed by the resin, which may affect test results. In addition, the incubation time can be shortened to 2 hours by determining positive and negative results through Coulter counting, rather than the traditional positive reporting method.

[0029] At the same time, the blood culture bottle of the present invention eliminates the sensor, which simplifies the manufacturing process and saves costs. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 This is a schematic diagram of the blood culture bottle of the present invention;

[0031] Figure 2Schematic diagram of a blood culture bottle without the cancellation of the receptor. DETAILED DESCRIPTION

[0032] The present invention provides a blood culture bottle capable of rapid and accurate detection, wherein the culture fluid in the blood culture bottle comprises brain heart infusion, SPS, activated carbon and hemolysin.

[0033] In the present invention, the added amounts of the brain heart infusion and SPS are 2.4g-5.6g and 0.5g-1.18g respectively.

[0034] In the present invention, the activated carbon is specifically an activated carbon fiber filter disc, and the diameter of the activated carbon fiber filter disc is 3.4 cm to 3.6 cm; the hemolysin is specifically saponin, and the added amount of the saponin is 0.018 g to 0.042 g.

[0035] The present invention incorporates an activated carbon fiber filter into the blood culture bottle. The activated carbon fiber, with its highly developed pore structure and large specific surface area, effectively absorbs impurities, metabolites, and substances that may inhibit microbial growth, such as fatty acids and bile salts in the blood. These substances, if not removed, may interfere with microbial growth or even inhibit their reproduction. Adsorption creates a more favorable growth environment for microorganisms.

[0036] Furthermore, activated carbon fiber has certain adsorption and release properties for gases, which can regulate the gas environment in the blood culture bottle to a certain extent. It can absorb excess oxygen in the bottle, creating a micro-oxygen or anaerobic environment suitable for the growth of some anaerobic bacteria. It can also absorb gases such as carbon dioxide, maintaining the relative stability of the gas composition in the bottle, which is conducive to the growth and metabolism of microorganisms.

[0037] In addition, activated carbon fiber can absorb immune cells, antibodies and other immune-related substances that may exist in the blood, reduce their attack and inhibition on microorganisms, provide a relatively safe living space for microorganisms, and help improve the detection rate of microorganisms. In addition, it can also absorb some harmful metabolites produced by microorganisms during the culture process, avoiding the accumulation of these products and having a negative impact on the growth of the microorganisms themselves.

[0038] The activated carbon fiber filter of the present invention allows microorganisms to grow in a more suitable environment by adsorbing impurities and regulating the gas environment, thereby reducing the interference of external factors on microbial growth. As a result, the culture results can more accurately reflect the situation of microorganisms in the blood, improve the accuracy and reliability of blood culture testing, and reduce the probability of false negative or false positive results.

[0039] The present invention adds hemolysin to the blood culture bottle, which facilitates the differentiation of bacteria and blood cells. First, it can promote the release of microorganisms: there are a large number of blood cells in the blood, including red blood cells and white blood cells. Some microorganisms may be wrapped or engulfed by the blood cells, and placed in a relatively closed environment, which will affect their growth in the culture medium. Hemolysin can destroy the cell membrane of the red blood cells, allowing the microorganisms inside the cells to be released into the culture medium, increasing the contact area between the microorganisms and the culture medium, making it easier for them to obtain nutrients, thereby improving the detection rate of microorganisms and preventing blood cells from interfering with bacterial counts and affecting the test results.

[0040] Secondly, during the hemolysis process, the rupture of red blood cells releases a variety of nutrients within the cells, such as hemoglobin, amino acids, and iron ions. These substances can provide rich nutrients for the growth of microorganisms, help meet the nutritional needs of microbial growth, promote their growth and reproduction, and enable microorganisms that were originally nutrient-limited in the blood to grow better in the culture bottle, facilitating subsequent detection;

[0041] In addition, hemolysin causes red blood cells to rupture, which can reduce color interference in blood samples and make it easier to observe changes in culture medium (such as turbidity, etc.) caused by microbial growth. On the other hand, substances such as hemoglobin released after hemolysis may react specifically with certain detection reagents and can serve as an indirect indicator of microbial growth, helping to more accurately judge blood culture results and improve the sensitivity and accuracy of detection.

[0042] In the present invention, the culture solution further comprises tryptone soy broth, tryptone, yeast powder, yeast nitrogen source, glucose, sucrose, sodium hydroxide, hemin chloride, vitamin K and pure water.

[0043] Tryptone soy broth is the basic culture medium for blood culture bottles. It provides rich nitrogen sources such as polypeptides and amino acids, providing necessary nutrients for the growth of microorganisms. Soy peptone contains a variety of vitamins, minerals, etc., which can meet the various needs of microbial growth. The broth ingredients contain some soluble nutrients such as carbohydrates, which can provide carbon sources and other nutrients for microbial growth, and contribute to the growth and reproduction of various microorganisms.

[0044] The present invention provides nitrogen source through tryptone, which is a product obtained by digesting protein with trypsin. It contains various amino acids and can be directly used by microorganisms to synthesize their own proteins and other nitrogen-containing biomolecules, thereby promoting the growth of microorganisms.

[0045] Yeast powder is rich in various nutrients such as vitamins, amino acids, nucleotides, etc. Among them, B vitamins can serve as precursors of coenzymes or cofactors required for microbial growth and participate in the metabolic process of microorganisms; amino acids provide nitrogen sources for microorganisms, and nucleotides can provide raw materials for microorganisms to synthesize nucleic acids, thereby promoting the growth and reproduction of microorganisms.

[0046] Yeast nitrogen source provides nitrogen source for microbial growth and may also contain some trace elements and vitamins necessary for microbial growth. It can provide comprehensive nutritional support for the growth of microorganisms such as yeast and ensure the normal growth and metabolism of microorganisms in blood culture bottles.

[0047] Glucose is an important carbon source. Microorganisms can break down glucose to generate energy, which they use to sustain their own life activities, such as cell division and biosynthesis. Glucose also serves as a carbon skeleton for the synthesis of other organic substances within microbial cells.

[0048] Sucrose is also a carbon source. Some microorganisms can secrete sucrase to break down sucrose into glucose and fructose, and then use them to provide energy and carbon sources. Compared with glucose, sucrose has better stability and can supplement and slowly release carbon sources in the culture medium, allowing microorganisms to continuously obtain carbon source supply for a longer period of time.

[0049] The present invention also adds sodium hydroxide, which is mainly used to adjust the pH value of the culture medium. The growth of microorganisms requires a suitable pH environment. Different microorganisms have different requirements for pH. Sodium hydroxide can be used to adjust the pH value of the culture medium to a range suitable for microbial growth, so as to ensure the enzyme activity and cell structure stability of the microorganisms and promote the growth of microorganisms.

[0050] As for hemin chloride, many microorganisms need heme substances to synthesize cytochromes and other respiratory chain-related enzymes during their growth process, participate in cellular respiration, and thus obtain energy. Hemin chloride can provide iron for microorganisms. At the same time, as a precursor of hemoglobin, it can meet the microorganism's demand for hemoglobin and promote its growth. Especially for some bacteria such as Haemophilus that have special requirements for hemoglobin, hemin chloride is an indispensable component in blood culture bottles.

[0051] In addition, vitamin K is a nutrient necessary for the growth of some bacteria. Vitamin K participates in the electron transfer and redox reaction of bacteria and plays an important role in the respiratory metabolism of bacteria. In addition, it may also be related to the synthesis of the cell wall of some bacteria, helping to maintain the integrity and stability of bacterial cells, thereby promoting the growth and reproduction of bacteria.

[0052] In the present invention, the addition amounts of the tryptone soy broth, tryptone, yeast powder, yeast nitrogen source, glucose, sucrose, sodium hydroxide, hemin chloride, vitamin K, and pure water are preferably 10.8g-25.2g, 4.8g-11.2g, 0.15g-0.35g, 0.15g-0.35g, 1.2g-2.8g, 2.1g-4.9g, and 0.48g, respectively. ~1.12g, 0.003g~0.007g, 0.0003g~0.0007g, 1L, more preferably 18~25.2g, 8~11.2g, 0.2~0.35g, 0.2~0.35g, 1.6~2.8g, 2.8~3.5g, 0.8~1.12g, 0.003~0.005g, 0.003~0.005g, 1L.

[0053] In the present invention, the concentration of the saponin is preferably 0.05% to 0.1%, more preferably 0.05 to 0.08%.

[0054] In the present invention, the pH of the culture solution is preferably 7.0 to 7.4, more preferably 7.0 to 7.2.

[0055] The present invention also provides a method for preparing a blood culture bottle capable of rapid and accurate detection as described in the above technical solution, the steps of the preparation method are:

[0056] Mixing the raw materials in sequence to obtain a mixture;

[0057] The mixture was filtered through a microporous membrane and an activated carbon fiber filter was added to the culture medium, 30 mL of which was added to a PC bottle, and the bottle was sealed;

[0058] Then, the blood culture bottle capable of rapid and accurate detection is obtained after high pressure treatment at 121° C. for 15 to 20 minutes.

[0059] In the present invention, tryptone soy broth, tryptone, yeast powder, yeast nitrogen source, glucose, sucrose, sodium hydroxide, hemin chloride, vitamin K, pure water and saponin are mixed.

[0060] In the present invention, the microporous filter membrane is a microporous filter membrane with a thickness of 0.22 mm to 0.25 mm.

[0061] After the blood culture bottle is obtained, the present invention uses Coulter counting to determine the positive or negative result during use, thereby avoiding the problems of traditional blood culture bottles requiring long culture time and the possibility that the blood cells are not completely adsorbed by the resin, which may affect the test results. In addition, the culture time can be shortened to 2 hours by determining the positive or negative result through Coulter counting instead of the traditional positive reporting method.

[0062] In the present invention, unless otherwise specified, the raw materials required for preparation are all commercially available products well known to those skilled in the art.

[0063] The technical solutions provided by the present invention are described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0064] Examples 1 to 5

[0065] (1) Cleaning preparation: ultrasonically clean the container and rinse with pure water to ensure it is clean;

[0066] (2) Weighing: weigh the raw materials according to the requirements of the formula;

[0067] (3) dissolving and mixing tryptone soy broth, tryptone, yeast powder, yeast nitrogen source, glucose, sucrose, sodium hydroxide, hemin chloride, vitamin K, purified water, and saponin to obtain a dissolving solution;

[0068] (4) filtering the dissolved solution twice using a microporous filter membrane with a diameter of 0.1 μm to 1 μm to obtain a culture medium;

[0069] (5) Packaging: Packaging the prepared culture medium according to the filling volume requirements to obtain culture bottles;

[0070] (6) Quality inspection: The prepared culture bottles are inspected for physical and chemical indicators, and qualified culture bottles are reserved;

[0071] The amounts of different components in different formulations are shown in Table 1. Examples 1 to 5 correspond to formulations A, B, C, D, and E, respectively;

[0072] Table 1 Dosage of different components in different formulas

[0073]

[0074]

[0075] Example 6

[0076] The other steps are the same as those of Examples 1 to 5, except that the main components are weighed according to the different formulations in Table 1, and the pore size of the filter membrane used for filtering the dissolved substance twice can be selected from 0.1 μm to 1 μm.

[0077] Test Case

[0078] (1) Prepare a culture medium according to the preparation method described in Examples 1 to 5;

[0079] (2) Ultrasonic cleaning and pure water rinsing of the container to ensure that the container is clean and avoid contamination during the entire experiment;

[0080] (3) The physical and chemical indicators and pH values of the above culture medium were tested to see if they met the requirements. The results are shown in Table 2.

[0081] (4) Bacteria with a colony size of 1.5 were selected for colony counting. After the measurement, the bacteria were added to the culture bottles of each group and cultured for 2 hours. During this period, the colony count was performed every half hour and the test results were recorded. The results are shown in Table 3.

[0082] Table 2 Physical and chemical index determination results of culture medium obtained in Examples 1 to 5

[0083] index Required indicators Group A Group B Formula C D group Formula E pH value 7.0~7.8 (7.2 is the best) 7.62 7.43 7.2 7.0 7.64

[0084] Table 3 Results of colony determination on culture medium obtained in Examples 1 to 3

[0085]

[0086] Based on Tables 2 and 3, it can be seen that the formulations of the present invention can meet the requirements of the bacteria counter for physical and chemical indicators.

[0087] Application Example 1

[0088] (1) Six common standard strains (ATCC 25922 Escherichia coli, ATCC 25923 Staphylococcus aureus, ATCC 27853 Pseudomonas aeruginosa, ATCC 29212 Enterococcus faecalis, ATCC 29213 Staphylococcus aureus, ATCC 700603 Klebsiella pneumoniae) were transferred and incubated at 37°C for 18 hours;

[0089] (2) Prepare the strain and grind it on the wall of the AST (antibiotic susceptibility test) broth bottle, mix it evenly, cover the bottle cap and measure the turbidity with a turbidimeter. The turbidity should be 0.5 McFarland units. Set aside. Pipette 5-30 bacterial strains of the bacterial solution and add it to the blood culture bottle obtained in Example 1 to obtain a positive bottle sample.

[0090] (3) The positive bottle samples were cultured and confirmed to be positive by Coulter counting. The results are shown in Table 4.

[0091] Table 4 Culture results of six different strains

[0092]

[0093]

[0094] Based on Table 4, it can be seen that the culture bottle of the present invention can normally culture the sample and can correctly report positive.

[0095] Comparative Example 1

[0096] Comparison of the performance differences between traditional culture bottles and the blood culture bottles of the present invention in positive detection

[0097] (1) Prepare a conventional blood culture bottle; a commercially available standard blood culture bottle containing a nutrient medium and a CO2 sensing bottom membrane; prepare the blood culture bottle obtained in Example 1 of the present invention, remove the bottom membrane, and use the Coulter counting method to detect bacterial growth.

[0098] Prepare blood samples: Blood samples collected from clinical patients (known positive and negative controls);

[0099] Coulter counting device: used to detect the bacterial concentration in new culture bottles;

[0100] Constant temperature incubator: 37°C, used for culturing blood culture bottles.

[0101] Microbial identification equipment: used to confirm the type of microorganisms in positive samples

[0102] (2) Blood samples were collected from clinical patients, each blood sample was divided into two parts, and injected into a traditional blood culture bottle and a blood culture bottle of the present invention, respectively, with 5-10 mL of blood injected into each bottle; a positive control (blood sample known to contain common pathogens) and a negative control (sterile blood sample) were set; all blood culture bottles were placed in a constant temperature incubator and cultured at 37°C.

[0103] (3) Observe the color change of the bottom membrane of the conventional blood culture bottle and record the time of positive result (bottom membrane color change); use a Coulter counter to detect the bacterial concentration in the blood culture bottle of the present invention every 30 minutes and record the time when the bacterial concentration increases significantly; use a microbial identification device well known in the art to confirm the type of pathogen;

[0104] For traditional blood culture bottles: if the bottom membrane changes color, it is positive, and the time of color change is recorded;

[0105] For the blood culture bottle of the present invention: a Coulter counter detected a significant increase in bacterial concentration, which was considered positive. The time of concentration increase was recorded. The results are shown in Table 5.

[0106] Table 5 Comparison of culture status between traditional culture bottles and culture bottles of the present invention

[0107]

[0108] Based on Table 5, it can be seen that the blood culture bottle of the present invention performs better than the traditional culture bottle in terms of sensitivity, specificity and detection time.

[0109] The basic concepts have been described above. It will be apparent to those skilled in the art that the detailed disclosure above is merely illustrative and does not limit the present application. Although not explicitly stated herein, those skilled in the art may make various modifications, improvements, and amendments to the present application. Such modifications, improvements, and amendments are suggested in the present application and remain within the spirit and scope of the exemplary embodiments of the present application.

[0110] At the same time, this application uses specific terms to describe the embodiments of this application. For example, "one embodiment," "an embodiment," and / or "some embodiments" refer to a certain feature, structure, or characteristic related to at least one embodiment of this application. Therefore, it should be emphasized and noted that "one embodiment," "an embodiment," or "an alternative embodiment" mentioned twice or multiple times in different locations in this specification does not necessarily refer to the same embodiment. In addition, certain features, structures, or characteristics in one or more embodiments of this application may be appropriately combined.

[0111] Furthermore, those skilled in the art will appreciate that various aspects of the present application may be illustrated and described in terms of several patentable categories or situations, including any new and useful process, machine, product or composition of matter, or any new and useful improvement thereof.

[0112] In addition, unless explicitly stated in the claims, the order of the processing elements and sequences, the use of alphanumeric characters, or the use of other names described in this application are not intended to limit the order of the processes and methods of this application. Although the above disclosure discusses some of the invention embodiments currently considered useful through various examples, it should be understood that such details are only for illustrative purposes, and the appended claims are not limited to the disclosed embodiments. On the contrary, the claims are intended to cover all modifications and equivalent combinations that are consistent with the spirit and scope of the embodiments of this application.

[0113] Similarly, it should be noted that, in order to simplify the presentation of this application and thus facilitate understanding of one or more embodiments of the invention, the foregoing descriptions of the embodiments of this application sometimes combine multiple features into a single embodiment, figure, or description thereof. However, this disclosure method does not mean that the subject matter of this application requires more features than those recited in the claims. In fact, an embodiment may have fewer features than all of the features of a single embodiment disclosed above.

[0114] In some embodiments, numbers are used to describe the quantity of components and attributes. It should be understood that such numbers used in the description of the embodiments are modified by the modifiers "about", "approximately" or "substantially" in some examples. Unless otherwise stated, "about", "approximately" or "substantially" indicate that the numbers are allowed to vary by ±20%. Accordingly, in some embodiments, the numerical parameters used in the description and claims are approximate values, which may change according to the required features of individual embodiments. In some embodiments, the numerical parameters should take into account the specified significant digits and adopt the general method of retaining digits. Although the numerical domains and parameters used to confirm the breadth of their range in some embodiments of the present application are approximate values, in specific embodiments, the settings of such numerical values are as accurate as possible within the feasible range.

[0115] Each patent, patent application, patent application disclosure, and other materials, such as articles, books, specifications, publications, documents, etc., cited in this application is hereby incorporated by reference in its entirety. This includes application history documents that are inconsistent with or conflict with the content of this application, as well as documents (currently or subsequently attached to this application) that limit the broadest scope of the claims of this application. It should be noted that if the descriptions, definitions, and / or use of terms in the accompanying materials of this application are inconsistent or conflicting with the content of this application, the descriptions, definitions, and / or use of terms in this application shall prevail.

[0116] Finally, it should be understood that the embodiments described in this application are merely illustrative of the principles of the embodiments of this application. Other variations may also fall within the scope of this application. Therefore, by way of example and not limitation, alternative configurations of the embodiments of this application may be considered consistent with the teachings of this application. Accordingly, the embodiments of this application are not limited to the embodiments explicitly introduced and described in this application.

Claims

1. A blood culture bottle capable of rapid and accurate detection, characterized in that: The culture medium in the blood culture bottle includes brain heart infusion, SPS, activated carbon and hemolysin.

2. The blood culture bottle capable of rapid and accurate detection according to claim 1, characterized in that: The added amounts of the brain heart infusion and SPS are 2.4g-5.6g and 0.5g-1.18g respectively.

3. The blood culture bottle capable of rapid and accurate detection according to claim 1, characterized in that: The activated carbon is specifically an activated carbon fiber filter disc, and the diameter of the activated carbon fiber filter disc is 3.4 cm to 3.6 cm; the hemolysin is specifically saponin, and the added amount of the saponin is 0.018 g to 0.042 g.

4. The blood culture bottle capable of rapid and accurate detection according to claim 1, characterized in that: The culture solution also includes tryptone soy broth, tryptone, yeast powder, yeast nitrogen source, glucose, sucrose, sodium hydroxide, hemin chloride, vitamin K and purified water.

5. The blood culture bottle capable of rapid and accurate detection according to claim 4, characterized in that: The added amounts of the tryptone soy broth, tryptone, yeast powder, yeast nitrogen source, glucose, sucrose, sodium hydroxide, hemin chloride, vitamin K, and pure water are 10.8g-25.2g, 4.8g-11.2g, 0.15g-0.35g, 0.15g-0.35g, 1.2g-2.8g, 2.1g-4.9g, 0.48g-1.12g, 0.003g-0.007g, 0.0003g-0.0007g, and 1L, respectively.

6. The blood culture bottle capable of rapid and accurate detection according to claim 3, characterized in that: The concentration of the saponin is 0.05% to 0.1%.

7. The blood culture bottle capable of rapid and accurate detection according to claim 1, characterized in that: The pH of the culture solution is 7.0-7.

4.

8. A method for preparing a blood culture bottle capable of rapid and accurate detection according to any one of claims 1 to 7, characterized in that: The steps of the preparation method are: Mixing raw materials of tryptone soy broth, tryptone, yeast powder, yeast nitrogen source, glucose, sucrose, sodium hydroxide, hemin chloride, vitamin K, and purified water to obtain a mixture; The mixture was filtered through a microporous membrane and an activated carbon fiber filter was added to the culture medium, 30 mL of which was added to a PC bottle, and the bottle was sealed; Then, the blood culture bottle capable of rapid and accurate detection is obtained after high pressure treatment at 121° C. for 15 to 20 minutes.

9. The method for preparing a blood culture bottle capable of rapid and accurate detection according to claim 8, characterized in that: The tryptone soy broth, tryptone, brain heart infusion, yeast powder, yeast nitrogen source, glucose, sucrose, SPS, sodium hydroxide, hemin chloride, vitamin K, purified water and saponin are mixed.

10. The method for preparing a blood culture bottle capable of rapid and accurate detection according to claim 8, characterized in that: The microporous filter membrane is a microporous filter membrane with a diameter of 0.22 mm to 0.25 mm.

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