Multiple-fluorescence double-sample POCT (Point of Care Testing) optical detection system
By designing a multi-fluorescence dual-sample POCT optical detection system with a common optical path excitation light source module and a spectroscopic prism, the problems of low multi-sample detection efficiency and poor stability in the prior art are solved, and efficient and accurate multi-fluorescence detection is achieved, miniaturizing the system and signal enhancement.
Patent Information
- Application Number
- CN202510581954.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-05-07
- Publication Date
- 2025-08-08
AI Technical Summary
The existing optical detection system is difficult to detect multiple samples at the same time, and the mechanical motion structure leads to poor stability, inability to miniaturize, low detection efficiency, lack of control groups, and weak fluorescence signal.
A multi-fluorescence dual-sample POCT optical detection system is designed, using a common optical path excitation light source module and a spectroscopic prism to divide the excitation light into two beams perpendicular to each other into the dual-sample slot, combining multi-channel excitation and detection, and using a reflector to enhance the fluorescence signal to realize synchronous detection of the two samples.
Multi-channel multi-fluorescence detection is realized, which improves detection efficiency and accuracy, has high system stability and no mechanical components, which can achieve miniaturization and enhances the intensity of fluorescence signal.
Smart Images
Figure CN120446066A_ABST
Abstract
Description
Technical Field
[0001] The present invention belongs to the field of optical detection technology, and in particular relates to a multiple fluorescence dual-sample POCT optical detection system. Background Art
[0002] Point-of-care testing (POCT) refers to a method that uses portable analytical instruments and supporting reagents to quickly obtain test results at the sampling site. The operating principle of the multi-fluorescence POCT optical detection system is as follows: Multiple specific wavelengths of excitation light are first applied to the sample tube to be tested, causing the reagents in the sample tube to emit fluorescence at specific wavelengths. This fluorescence is then filtered, and finally, a photosensitive element is used to collect the emitted fluorescence and perform photoelectric signal processing in real time.
[0003] A typical fluorescence POCT optical detection system generally consists of one or two excitation light channels and a corresponding number of receiving channels, and can only perform detection on a single sample. Some systems also use a mechanical turntable to control the filter switching, or use a motor to drive the photosensitive element to collect signals. However, the current optical path system has the following defects: on the one hand, the number of excitation light channels is scarce, making it difficult to detect multi-dye tube samples; on the other hand, due to the existence of a mechanical motion structure, the stability of the experimental results is poor, and the numerous mechanical components make it impossible to achieve the goals of miniaturization and modularization; in addition, the single sample detection mode is inefficient and lacks a control group. Coupled with the weak fluorescence signal, it brings considerable difficulties to the detection work. In view of this, the present invention proposes a multiple fluorescence dual-sample POCT optical detection system. Summary of the Invention
[0004] The object of the present invention is to provide a multiple fluorescence dual-sample POCT optical detection system, aiming to solve the problems raised in the above background technology.
[0005] The purpose of the present invention is achieved through the following technical solutions:
[0006] A multiple fluorescence dual-sample POCT optical detection system includes an excitation light source module, a sample tank, a beam splitter placed between the excitation light source module and the sample tank, a reflector for deflecting the light path, and a fluorescence detection module;
[0007] The sample slots include a first sample slot and a second sample slot, and the first sample slot and the second sample slot have the same structure, both including a sample slot body, a first plane reflector, and a second plane reflector. A sample to be tested is placed in the sample slot body, and the first plane reflector and the second plane reflector are placed at the upper and lower ends of the sample slot body, respectively. The excitation light emitted by the excitation light source module is divided into two mutually perpendicular beams of excitation light by a beam splitter prism, and enters the first sample slot and the second sample slot, respectively. After the samples to be tested in the first sample slot and the second sample slot absorb the excitation light, they are stimulated to emit fluorescence, and the fluorescence is reflected and enhanced by the first plane reflector and the second plane reflector before entering the fluorescence detection module.
[0008] The fluorescence detection module includes a first sample fluorescence detection module and a second sample fluorescence detection module, each of which includes three groups of detection units, which are respectively located on three sides of the sample tank, wherein one group of detection units is coaxial with the excitation light incident on the sample tank, and the axis directions of the other two groups of detection units are perpendicular to the excitation light axis incident on the sample tank; each group of detection units includes a converging lens, a filter, and a photodetector, which are coaxial and arranged in sequence; the fluorescence reflected and enhanced by the plane reflector 1 and the plane reflector 2 respectively enters the photodetectors of the three groups of detection units to complete signal conversion and acquisition;
[0009] The first sample slot and the second sample slot are filled with samples of the same type to be tested, or one of the sample slots is filled with a sample without a fluorescent group as a control group, and the other sample slot is filled with the sample to be tested as an experimental group.
[0010] Furthermore, the excitation light source module includes a first channel excitation light source, a second channel excitation light source, a third channel excitation light source, a dichroic mirror 1, a dichroic mirror 2, a filter 1, a filter 2, a filter 3, a collimating lens 1, a collimating lens 2 and a collimating lens 3; the dichroic mirror 1 and the dichroic mirror 2 are long-wave pass, that is, they reflect the short-wave band and transmit the long-wave band; the first channel excitation light source, the second channel excitation light source and the third channel excitation light source are set as medium, short and long-wave LED light sources respectively.
[0011] Furthermore, the first channel excitation light source emits a medium-band excitation light, which passes through filter 1 and collimating lens 1 and enters dichroic mirror 1. The medium-band excitation light passes through dichroic mirror 1 and enters dichroic mirror 2. The medium-band excitation light is reflected by dichroic mirror 2 to the beam splitter prism; the second channel excitation light source emits a short-band excitation light, which passes through filter 2 and collimating lens 2 and enters dichroic mirror 1. The short-band excitation light is reflected by dichroic mirror 1 and enters dichroic mirror 2. The short-band excitation light is reflected by dichroic mirror 2 to the beam splitter prism; the third channel The excitation light source emits long-wavelength excitation light, which passes through filter three and collimating lens three and enters dichroic mirror two. The long-wavelength excitation light passes through dichroic mirror two to the beam splitter prism; the medium-wavelength excitation light, short-wavelength excitation light and long-wavelength excitation light enter the beam splitter prism along the same optical path; the combined three-channel excitation light is transmitted and reflected by the beam splitter prism and is divided into two mutually perpendicular and identical beams of excitation light. The excitation light beam coaxial with the three-channel excitation light is deflected by the reflector and enters the first sample tank, and the excitation light beam perpendicular to the three-channel excitation light enters the second sample tank.
[0012] Furthermore, the first sample fluorescence detection module includes a first channel detector of the first sample slot, a second channel detector of the first sample slot, a third channel detector of the first sample slot, filter four, filter five, filter six, converging lens one, converging lens two and converging lens three; the converging lens one, filter four and the first channel detector of the first sample slot constitute a group of detection units in the first sample fluorescence detection module that are perpendicular to the excitation light axis of the incident sample slot; the converging lens three, filter six and the third channel detector of the first sample slot constitute another group of detection units in the first sample fluorescence detection module that are perpendicular to the excitation light axis of the incident sample slot; the converging lens two, filter five and the second channel detector of the first sample slot constitute a group of detection units in the first sample fluorescence detection module that are coaxial with the excitation light of the incident sample slot.
[0013] Furthermore, the fluorescence enhanced by reflection by plane mirror 1 and plane mirror 2 of the first sample slot enters the first channel detector of the first sample slot through the converging lens 1 and filter 4, enters the second channel detector of the first sample slot through the converging lens 2 and filter 5, and enters the third channel detector of the first sample slot through the converging lens 3 and filter 6.
[0014] Furthermore, the second sample fluorescence detection module includes a first channel detector of the second sample slot, a second channel detector of the second sample slot, a third channel detector of the second sample slot, filter seven, filter eight, filter nine, converging lens four, converging lens five and converging lens six; the converging lens four, filter seven and the first channel detector of the second sample slot constitute a group of detection units in the second sample fluorescence detection module that are perpendicular to the excitation light axis of the incident sample slot; the converging lens six, filter nine and the third channel detector of the second sample slot constitute another group of detection units in the second sample fluorescence detection module that are perpendicular to the excitation light axis of the incident sample slot; the converging lens five, filter eight and the second channel detector of the second sample slot constitute a group of detection units in the second sample fluorescence detection module that are coaxial with the excitation light of the incident sample slot.
[0015] Furthermore, the fluorescence enhanced by reflection by plane mirror 1 and plane mirror 2 of the second sample slot enters the first channel detector of the second sample slot through the converging lens 4 and filter 7, enters the second channel detector of the second sample slot through the converging lens 5 and filter 8, and enters the third channel detector of the second sample slot through the converging lens 6 and filter 9.
[0016] Compared with the prior art, the present invention has the following beneficial effects:
[0017] 1. The present invention designs a common optical path excitation light source module, integrates multi-band light sources, and realizes beam combining and common path transmission through the coordination of optical components. It can carry out multi-channel multiple fluorescence detection and accurately identify and detect multi-stained tube samples.
[0018] 2. The present invention designs a splitting optical path, using a splitter prism to split the co-excitation light source beam into a dual sample tank, combining multi-channel excitation and detection, and dual-sample synchronous multiple fluorescence detection, with high detection efficiency.
[0019] 3. The present invention realizes a double-tube co-excitation light path and multiple fluorescence excitation. The sample slot can measure two samples at the same time and can also conduct sample group and control group experiments at the same time, thereby improving the detection accuracy.
[0020] 4. There is no relative movement between the components of the present invention, the optical detection system has no mechanical components, and has high stability.
[0021] 5. The optical path design of the present invention is compact, which enables the miniaturization of the detection system.
[0022] 6. The sample tank of the present invention is designed with reflectors at both ends. The sample's stimulated fluorescence is reflected multiple times in the tank, and the emitted fluorescence signal is enhanced by self-reflection, thereby improving detection accuracy. BRIEF DESCRIPTION OF THE DRAWINGS
[0023] Figure 1 This is a diagram of the optical element arrangement of the present invention.
[0024] Figure 2 Schematic diagram of the sample tank in the present invention.
[0025] Figure 3 Schematic diagram of the light path of the excitation light incident on the first sample tank and the second sample tank of the present invention.
[0026] Figure 4 Schematic diagram of the component arrangement and light path of the first sample fluorescence detection module in the present invention.
[0027] In the figure: first channel excitation light source 1, second channel excitation light source 2, third channel excitation light source 3, first sample slot first channel detector 4, first sample slot second channel detector 5, first sample slot third channel detector 6, second sample slot first channel detector 7, second sample slot second channel detector 8, second sample slot third channel detector 9, dichroic mirror 1 10, dichroic mirror 2 11, beam splitter 12, filter 1 13, filter 2 14, filter 3 15 , filter four 16, filter five 17, filter six 18, filter seven 19, filter eight 20, filter nine 21, collimating lens one 22, collimating lens two 23, collimating lens three 24, converging lens one 25, converging lens two 26, converging lens three 27, converging lens four 28, converging lens five 29, converging lens six 30, sample tank body 31, plane reflector one 32, plane reflector two 33, reflector 34, first sample tank A, second sample tank B. DETAILED DESCRIPTION
[0028] In order to have a clearer understanding of the technical features, objectives and beneficial effects of the present invention, the technical solution of the present invention is now described in detail below, but it should not be understood as limiting the scope of implementation of the present invention.
[0029] The specific implementation of the present invention is described in detail below with reference to specific embodiments.
[0030] like Figures 1-4 As shown, one embodiment of the present invention provides a multiple fluorescence dual-sample POCT optical detection system, including an excitation light source module, a sample slot, a dichroic prism (50:50 dichroic prism) 12 placed between the excitation light source module and the sample slot, a reflector 34 for refracting the light path, and a fluorescence detection module.
[0031] The excitation light source module includes a first channel excitation light source 1, a second channel excitation light source 2, a third channel excitation light source 3, a dichroic mirror 10, a dichroic mirror 2 11, a filter 13, a filter 2 14, a filter 3 15, a collimating lens 1 22, a collimating lens 2 23, and a collimating lens 3 24. The dichroic mirrors 10 and 11 are long-wavelength pass filters, reflecting short-wavelength wavelengths and transmitting long-wavelength wavelengths. The first channel excitation light source 1, the second channel excitation light source 2, and the third channel excitation light source 3 are configured as medium-, short-, and long-wavelength LED light sources, respectively.
[0032] The sample tank includes a first sample tank A and a second sample tank B. The first sample tank A and the second sample tank B have the same structure, including a sample tank body 31, a plane reflector 1 32, and a plane reflector 2 33. The sample to be tested is placed in the sample tank body 31, and the plane reflector 1 32 and the plane reflector 2 33 are respectively placed at the upper and lower ends of the sample tank body 31. The excitation light emitted by the excitation light source module is split into two mutually perpendicular excitation light beams by the beam splitter prism 12, and enters the first sample tank A and the second sample tank B respectively. After absorbing the excitation light, the samples to be tested in the first sample tank A and the second sample tank B are stimulated to emit fluorescence. After being reflected and enhanced by the plane reflector 1 32 and the plane reflector 2 33, the fluorescence enters the fluorescence detection module.
[0033] The fluorescence detection module includes a first sample fluorescence detection module and a second sample fluorescence detection module. Each of the first and second sample fluorescence detection modules includes three groups of detection units, which are located on three sides of the sample tank. One group of detection units is coaxial with the excitation light incident on the sample tank, and the axis direction of the other two groups of detection units is perpendicular to the excitation light axis incident on the sample tank. Each group of detection units includes a converging lens, a filter, and a photodetector, which are coaxial and arranged in sequence. The fluorescence, after being reflected and enhanced by plane reflector 1 32 and plane reflector 2 33, enters the photodetectors of the three detection units respectively, completing signal conversion and acquisition.
[0034] Specifically, the first sample fluorescence detection module includes a first sample slot first channel detector 4, a first sample slot second channel detector 5, a first sample slot third channel detector 6, a filter four 16, a filter five 17, a filter six 18, a converging lens one 25, a converging lens two 26 and a converging lens three 27; the converging lens one 25, the filter four 16 and the first sample slot first channel detector 4 constitute a group of detection units in the first sample fluorescence detection module that are perpendicular to the excitation light axis of the incident sample slot; the converging lens three 27, the filter six 18 and the first sample slot third channel detector 6 constitute another group of detection units in the first sample fluorescence detection module that are perpendicular to the excitation light axis of the incident sample slot; the converging lens two 26, the filter five 17 and the first sample slot second channel detector 5 constitute a group of detection units in the first sample fluorescence detection module that are coaxial with the excitation light of the incident sample slot.
[0035] The second sample fluorescence detection module includes a second sample slot first channel detector 7, a second sample slot second channel detector 8, a second sample slot third channel detector 9, a filter seven 19, a filter eight 20, a filter nine 21, a converging lens four 28, a converging lens five 29 and a converging lens six 30; the converging lens four 28, the filter seven 19 and the second sample slot first channel detector 7 constitute a group of detection units in the second sample fluorescence detection module that are perpendicular to the excitation light axis of the incident sample slot; the converging lens six 30, the filter nine 21 and the second sample slot third channel detector 9 constitute another group of detection units in the second sample fluorescence detection module that are perpendicular to the excitation light axis of the incident sample slot; the converging lens five 29, the filter eight 20 and the second sample slot second channel detector 8 constitute a group of detection units in the second sample fluorescence detection module that are coaxial with the excitation light of the incident sample slot.
[0036] In an embodiment of the present invention, the working principle of the multiple fluorescence dual-sample POCT optical detection system is as follows:
[0037] like Figure 3As shown, the first channel excitation light source 1 emits medium-band excitation light, which passes through filter 13 and collimating lens 1 22 and enters dichroic mirror 1 10. The medium-band excitation light passes through dichroic mirror 1 10 and enters dichroic mirror 2 11. The medium-band excitation light is reflected by dichroic mirror 2 11 to the dichroic prism 12; the second channel excitation light source 2 emits short-band excitation light, which passes through filter 2 14 and collimating lens 2 23 and enters dichroic mirror 1 10. The short-band excitation light is reflected by dichroic mirror 1 10 and enters dichroic mirror 2 11. The short-band excitation light is reflected by dichroic mirror 2 11 to the dichroic prism 12; the third channel excitation light source 3 emits long-band excitation light, which passes through filter 3 15 and collimating lens 3 24 and enters dichroic mirror 2 11. The long-band excitation light passes through dichroic mirror 2 11 to the dichroic prism 12. Adjust the positions and angles of the first-channel excitation light source 1, the second-channel excitation light source 2, and the third-channel excitation light source 3 so that the light beams are combined and share a common optical path after passing through the dichroic mirror 11. The combined three-channel excitation light is transmitted and reflected by the beam splitter prism 12, splitting it into two mutually perpendicular and identical excitation light beams. The excitation light beam coaxial with the three-channel excitation light is deflected by the reflector 34 and enters the first sample tank A, while the excitation light beam perpendicular to the three-channel excitation light enters the second sample tank B.
[0038] The principle of the first sample fluorescence detection module is the same as that of the second sample fluorescence detection module. Taking the first sample fluorescence detection module as an example, the working process of the sample tank and the detection module is described. Figure 4 As shown, the excitation light beam enters the first sample tank A, exciting the corresponding fluorescent groups in the sample, causing the sample to emit fluorescence under stimulation. The fluorescence is reflected multiple times by plane mirror 1 32 and plane mirror 2 33 in the sample tank body 31, completing the fluorescence signal enhancement. Then, the fluorescence passes through converging lens 1 25, converging lens 2 26, converging lens 3 27, filter 4 16, filter 5 17, and filter 6 18, and enters three photodetectors (photodiodes or photomultiplier tubes), namely, the first channel detector 4 of the first sample tank, the second channel detector 5 of the first sample tank, and the third channel detector 6 of the first sample tank. Specifically, the fluorescence enters the first channel detector 4 of the first sample tank through converging lens 1 25 and filter 4 16, enters the second channel detector 5 of the first sample tank through converging lens 2 26 and filter 5 17, and enters the third channel detector 6 of the first sample tank through converging lens 3 27 and filter 6 18.
[0039] Similarly, the second sample fluorescence detection module: the excitation light beam enters the second sample tank B, stimulating the corresponding fluorescent groups in the sample, causing the sample to emit fluorescence. The fluorescence is reflected multiple times in the sample tank body 31 by plane reflector 1 32 and plane reflector 2 33, completing the fluorescence signal enhancement. The fluorescence then passes through converging lens 4 28, converging lens 5 29, converging lens 6 30, filter 7 19, filter 8 20, and filter 9 21, entering three photodetectors (photodiodes or photomultiplier tubes): the first channel detector 7 of the second sample tank, the second channel detector 8 of the second sample tank, and the third channel detector 9 of the second sample tank. Specifically, the fluorescence passes through converging lens 4 28 and filter 7 19 into the first channel detector 7 of the second sample tank, passes through converging lens 5 29 and filter 8 20 into the second channel detector 8 of the second sample tank, and passes through converging lens 6 30 and filter 9 21 into the third channel detector 9 of the second sample tank, thereby completing signal conversion and acquisition.
[0040] like Figure 1 As shown, as a preferred embodiment of the present invention, the first sample well A and the second sample well B can be used to place similar samples to be tested, allowing for simultaneous dual-sample detection. Alternatively, one of the sample wells can be used as a control group, containing a sample without a fluorescent group. The first and second sample fluorescence detection modules simultaneously receive fluorescence signals from both the experimental and control groups, using the control group's photoelectric signal as a real-time background signal for signal processing by the experimental group's photoelectric detector, thereby improving detection accuracy.
[0041] like Figure 2 As shown, as a preferred embodiment of the present invention, in order to enhance the intensity of emitted fluorescent light, in addition to placing a plane reflector 1 32 and a plane reflector 2 33 at the upper and lower ends of the sample tank body 31, a reflective film layer can also be coated on the wall of the sample tank body 31 to enhance the fluorescence self-reflection effect.
[0042] The multiple fluorescence dual-sample POCT optical detection system proposed in the present invention has broad application prospects in the field of fluorescence detection, including but not limited to medical diagnosis, environmental pollution monitoring and food safety detection. Through its unique compact optical path design, this system not only achieves a significant reduction in the size of the device, making it convenient to carry and use, but also greatly reduces energy consumption and effectively extends battery life. In addition, the adopted multiple fluorescence design scheme not only improves the detection efficiency, but also ensures the accuracy and reliability of the test results. The highly integrated and modular structural design makes the maintenance and upgrade of the system simple and easy, further enhancing its practicality and flexibility in various application scenarios. It is worth mentioning that the system uses advanced multiple self-reflection technology to significantly enhance the fluorescence signal intensity of the sample's stimulated emission. This enables the detector to more effectively capture the fluorescence emitted by the sample, thereby achieving high-sensitivity fluorescence detection.
[0043] The above are only preferred embodiments of the present invention. It should be pointed out that for those skilled in the art, several variations and improvements can be made without departing from the concept of the present invention. These should also be regarded as the scope of protection of the present invention. These will not affect the effect of the implementation of the present invention and the practicality of the patent.
Claims
1. A multiplex fluorescence dual-sample POCT optical detection system, characterized in that: It includes an excitation light source module, a sample tank, a beam splitter placed between the excitation light source module and the sample tank, a reflector for deflecting the light path, and a fluorescence detection module; The sample slots include a first sample slot and a second sample slot, and the first sample slot and the second sample slot have the same structure, both including a sample slot body, a first plane reflector, and a second plane reflector. A sample to be tested is placed in the sample slot body, and the first plane reflector and the second plane reflector are placed at the upper and lower ends of the sample slot body, respectively. The excitation light emitted by the excitation light source module is divided into two mutually perpendicular beams of excitation light by a beam splitter prism, and enters the first sample slot and the second sample slot, respectively. After the samples to be tested in the first sample slot and the second sample slot absorb the excitation light, they are stimulated to emit fluorescence, and the fluorescence is reflected and enhanced by the first plane reflector and the second plane reflector before entering the fluorescence detection module. The fluorescence detection module includes a first sample fluorescence detection module and a second sample fluorescence detection module, each of which includes three groups of detection units, which are respectively located on three sides of the sample tank, wherein one group of detection units is coaxial with the excitation light incident on the sample tank, and the axis directions of the other two groups of detection units are perpendicular to the excitation light axis incident on the sample tank; each group of detection units includes a converging lens, a filter, and a photodetector, which are coaxial and arranged in sequence; the fluorescence reflected and enhanced by the plane reflector 1 and the plane reflector 2 respectively enters the photodetectors of the three groups of detection units to complete signal conversion and acquisition; The first sample slot and the second sample slot are filled with samples of the same type to be tested, or one of the sample slots is filled with a sample without a fluorescent group as a control group, and the other sample slot is filled with the sample to be tested as an experimental group.
2. The multiple fluorescence dual-sample POCT optical detection system according to claim 1, characterized in that: The excitation light source module includes a first channel excitation light source, a second channel excitation light source, a third channel excitation light source, a dichroic mirror 1, a dichroic mirror 2, a filter 1, a filter 2, a filter 3, a collimating lens 1, a collimating lens 2 and a collimating lens 3; the dichroic mirror 1 and the dichroic mirror 2 are long-wave pass, that is, they reflect the short-wave band and transmit the long-wave band; the first channel excitation light source, the second channel excitation light source and the third channel excitation light source are set as medium, short and long-wave LED light sources respectively.
3. The multiple fluorescence dual-sample POCT optical detection system according to claim 2, characterized in that: The first channel excitation light source emits a medium-band excitation light, which passes through filter 1 and collimating lens 1 and enters dichroic mirror 1. The medium-band excitation light passes through dichroic mirror 1 and enters dichroic mirror 2. The medium-band excitation light is reflected by dichroic mirror 2 to the beam splitter prism; the second channel excitation light source emits a short-band excitation light, which passes through filter 2 and collimating lens 2 and enters dichroic mirror 1. The short-band excitation light is reflected by dichroic mirror 1 and enters dichroic mirror 2. The short-band excitation light is reflected by dichroic mirror 2 to the beam splitter prism; the third channel excitation light The source emits long-wavelength excitation light, which passes through filter three and collimating lens three and enters dichroic mirror two. The long-wavelength excitation light passes through dichroic mirror two to the beam splitter prism; the medium-wavelength excitation light, short-wavelength excitation light and long-wavelength excitation light enter the beam splitter prism along the same optical path; the combined three-channel excitation light is transmitted and reflected by the beam splitter prism and is divided into two mutually perpendicular and identical excitation light beams. The excitation light beam coaxial with the three-channel excitation light is deflected by the reflector and enters the first sample tank, and the excitation light beam perpendicular to the three-channel excitation light enters the second sample tank.
4. The multiple fluorescence dual-sample POCT optical detection system according to claim 1, characterized in that: The first sample fluorescence detection module includes a first channel detector of the first sample slot, a second channel detector of the first sample slot, a third channel detector of the first sample slot, filter four, filter five, filter six, converging lens one, converging lens two and converging lens three; the converging lens one, filter four and the first channel detector of the first sample slot constitute a group of detection units in the first sample fluorescence detection module that are perpendicular to the excitation light axis of the incident sample slot; the converging lens three, filter six and the third channel detector of the first sample slot constitute another group of detection units in the first sample fluorescence detection module that are perpendicular to the excitation light axis of the incident sample slot; the converging lens two, filter five and the second channel detector of the first sample slot constitute a group of detection units in the first sample fluorescence detection module that are coaxial with the excitation light of the incident sample slot.
5. The multiple fluorescence dual-sample POCT optical detection system according to claim 4, characterized in that: The fluorescence reflected and enhanced by the plane reflector 1 and the plane reflector 2 of the first sample tank enters the first channel detector of the first sample tank through the converging lens 1 and the filter 4, enters the second channel detector of the first sample tank through the converging lens 2 and the filter 5, and enters the third channel detector of the first sample tank through the converging lens 3 and the filter 6.
6. The multiple fluorescence dual-sample POCT optical detection system according to claim 1, characterized in that: The second sample fluorescence detection module includes a first channel detector of the second sample slot, a second channel detector of the second sample slot, a third channel detector of the second sample slot, filter seven, filter eight, filter nine, converging lens four, converging lens five and converging lens six; the converging lens four, filter seven and the first channel detector of the second sample slot constitute a group of detection units in the second sample fluorescence detection module that are perpendicular to the excitation light axis of the incident sample slot; the converging lens six, filter nine and the third channel detector of the second sample slot constitute another group of detection units in the second sample fluorescence detection module that are perpendicular to the excitation light axis of the incident sample slot; the converging lens five, filter eight and the second channel detector of the second sample slot constitute a group of detection units in the second sample fluorescence detection module that are coaxial with the excitation light of the incident sample slot.
7. The multiple fluorescence dual-sample POCT optical detection system according to claim 6, characterized in that: The fluorescence enhanced by the reflection of the plane mirror 1 and the plane mirror 2 of the second sample tank enters the first channel detector of the second sample tank through the converging lens 4 and the filter 7, enters the second channel detector of the second sample tank through the converging lens 5 and the filter 8, and enters the third channel detector of the second sample tank through the converging lens 6 and the filter 9.