Chromogenic reaction detection method of portable borax rapid detection kit

Abstract

The invention relates to the technical field of detection kits, in particular to a chromogenic reaction detection method of a portable rapid borax detection kit.The method comprises the steps that 2 g of a sample is weighed and cut into pieces, 5 mL of distilled water is added, oscillation is carried out for multiple times, standing is carried out for 5 min, then supernate is taken as sample to-be-detected liquid, a piece of borax test paper is taken, sample soaking liquid is dropped to the middle of the test paper, and the sample to-be-detected liquid is obtained; then dropping a drop of curcumin solution at the same position, if reddish brown spots appear on the test paper, dropping a drop of hydrochloric acid on the spots, and if the test paper becomes green black or bluish violet, judging that the sample is doped with borax; the curcumin solution is used as a color developing agent, the curcumin solution and boric acid generate a specific reddish brown complex under the acidic condition, hydrochloric acid is used for reinforcing the color developing reaction, and the temperature-controlled electric hair drier is used for accelerating the drying of the test paper, so that the detection time is shortened to 5-10 minutes; therefore, the detection sensitivity is effectively improved, the anti-interference capability is enhanced, and the operation convenience and the application universality are greatly improved.

Description

Technical Field

[0001] The invention relates to the technical field of detection kits, in particular to a color development reaction detection method of a portable borax rapid detection kit. Background Art

[0002] Borax, as an industrial chemical, has antiseptic and gluten-enhancing effects and is often illegally added to foods such as aquatic products, meat products, and pasta to improve texture or extend shelf life. However, borax is significantly toxic to the human body. Long-term intake can lead to loss of appetite, indigestion, liver and kidney damage, and even multiple organ failure. GB2760, China's "National Food Safety Standard", clearly stipulates that borax shall not be used as a food additive. However, due to its low cost and significant effect, the use of borax as an additive still persists in some areas, posing a serious threat to people's health and safety.

[0003] At present, the main methods for borax detection are spectroscopy, chromatography, chemical titration, etc. Although the above methods are highly accurate, they have problems such as high equipment cost, complex operation, and poor timeliness. They are difficult to adapt to the needs of grassroots market supervision, food company self-inspection, and consumer on-site rapid testing.

[0004] Therefore, in order to solve the above problems, the present invention proposes a colorimetric reaction detection method for a portable borax rapid detection kit. Summary of the Invention

[0005] In order to overcome the problems of poor timeliness and complicated operation of existing borax detection methods, the present invention proposes a colorimetric reaction detection method of a portable borax rapid detection kit.

[0006] The technical solution of the present invention is: a color reaction detection method of a portable borax rapid detection kit, comprising:

[0007] S1, processing the sample to be tested to prepare a sample solution to be tested;

[0008] S2, take an appropriate amount of the solution to be tested and add it to the borax test paper in the kit;

[0009] S3, adding a color developing reagent to the borax test paper;

[0010] S5. Determine the borax content based on the color change and compare it with the standard color chart.

[0011] Preferably, the color developing reagent is a curcumin solution composed of 0.35 mol / L curcumin + 3% hydrochloric acid. Curcumin combines with borax under acidic conditions to form a red complex for color development reaction with borax.

[0012] Preferably, the sample treatment requires weighing 2 g of sample and chopping it into pieces, then adding 5 mL of distilled water, shaking it several times, and taking the supernatant as the sample solution after standing for 5 minutes.

[0013] Preferably, step S2 comprises: first taking a piece of borax test paper, then using a plastic straw to draw 1 drop of sample soaking liquid into the middle of the test paper, and the amount of sample soaking liquid added is 1-2 ml.

[0014] Preferably, in step S3, one drop of curcumin solution is added to the same position where the sample soaking solution is added, and then the two solutions are evenly mixed.

[0015] Preferably, after step S3 is completed, pinch a corner of the test paper with your hands or tweezers and blow dry it with hot air from a hair dryer.

[0016] Preferably, if no reddish-brown spots appear on the test paper, the operation can be stopped. If reddish-brown spots appear on the test paper, drop one drop of 3-6 mol / L hydrochloric acid on the spots and observe the color change of the test paper.

[0017] Preferably, after drying with a hair dryer, no reddish-brown spots appear in the center of the droplet, which can be used to determine that the sample does not contain borax. After adding 3-6 mol / L hydrochloric acid, the test paper does not change color, which can also be used to determine that the sample does not contain borax. After drying with a hair dryer, reddish-brown spots appear in the center of the droplet, and after adding 3-6 mol / L hydrochloric acid, the test paper turns green-black or blue-purple, which can be used to determine that the sample is mixed with borax.

[0018] Preferably, the temperature of the hair dryer is controlled at 60-70° C., the wind speed is medium-speed, and the test paper must be blown through before the result can be judged based on its color.

[0019] Preferably, the standard colorimetric card includes multiple color scales from colorless to deep red, corresponding to borax concentrations of 0-100 mg / L respectively.

[0020] Beneficial effects of the present invention:

[0021] The present invention adopts curcumin solution as a color developer to generate a specific reddish-brown complex with boric acid under acidic conditions, supplemented with 3-6 mol / L hydrochloric acid to enhance the color development reaction, and accelerates the drying of the test paper by a temperature-controlled hair dryer, thereby shortening the detection time to 5-10 minutes, thereby effectively improving the sensitivity of the detection, enhancing the anti-interference ability, and greatly improving the convenience of operation and the wide range of applications. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 What is shown is a schematic diagram of the workflow of the present invention. DETAILED DESCRIPTION

[0023] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without making creative work are within the scope of protection of the present invention.

[0024] See also Figure 1 The present invention provides an embodiment: a color reaction detection method of a portable borax rapid detection kit, comprising:

[0025] S1, processing the sample to be tested to prepare a sample solution to be tested;

[0026] S2, take an appropriate amount of the solution to be tested and add it to the borax test paper in the kit;

[0027] S3, adding a color developing reagent to the borax test paper;

[0028] S5. Determine the borax content based on the color change and compare it with the standard color chart.

[0029] The color developing reagent is a curcumin solution composed of 0.35 mol / L curcumin + 3% hydrochloric acid. Curcumin combines with borax under acidic conditions to form a red complex for color development reaction with borax.

[0030] The sample treatment required weighing 2 g of sample and cutting it into pieces, then adding 5 mL of distilled water, shaking several times, and taking the supernatant as the sample solution after standing for 5 minutes.

[0031] The step S2 comprises: first taking a piece of borax test paper, then using a plastic straw to draw 1 drop of sample soaking liquid into the middle of the test paper, and the amount of sample soaking liquid added is 1-2 ml.

[0032] In step S3, one drop of curcumin solution is added to the same position where the sample soaking solution is added, and then the two solutions are evenly mixed.

[0033] After step S3 is completed, pinch a corner of the test paper with your hands or tweezers and blow dry it with hot air from a hair dryer.

[0034] If no reddish-brown spots appear on the test paper, stop the operation. If reddish-brown spots appear on the test paper, add one drop of 3-6 mol / L hydrochloric acid to the spots and observe the color change of the test paper.

[0035] After drying with a hair dryer, no red-brown spots appear in the center of the droplet, which means the sample does not contain borax. After adding 3-6mol / L hydrochloric acid, the test paper does not change color, which also means the sample does not contain borax. After drying with a hair dryer, red-brown spots appear in the center of the droplet, and after adding 3-6mol / L hydrochloric acid, the test paper turns green-black or blue-purple, which means the sample is mixed with borax.

[0036] The temperature of the hair dryer is controlled at 60-70°C, the wind speed is medium wind, and the test paper must be blown through before the result can be judged according to its color.

[0037] The standard colorimetric card includes multiple color scales from colorless to deep red, corresponding to borax concentrations of 0-100 mg / L respectively.

[0038] Further, the workflow of the present invention is described, specifically:

[0039] First, weigh 2g of sample and cut it into pieces, then add 5mL of distilled water and shake it several times to make it fully blend. After standing for 5 minutes, take the supernatant as the sample test solution, take a borax test paper, use a plastic straw to draw 1 drop of sample soaking solution to the middle of the test paper, and then drop 1 drop of curcumin solution in the same position, try to mix the two solutions evenly. After mixing, pinch a corner of the test paper with your hands or tweezers, and then blow dry with hot air from a hair dryer. If no reddish-brown spots appear on the test paper, you can stop the operation and it can be judged that the sample does not contain borax. After adding 3-6mol / L hydrochloric acid, the color of the test paper does not change, which can also be judged that the sample does not contain borax; if reddish-brown spots appear on the test paper, drop 1 drop of 3-6mol / L hydrochloric acid on the spots, and the test paper turns green-black or blue-purple, it can be judged that the sample may be mixed with borax.

[0040] The present invention provides an embodiment for testing purchased beef balls:

[0041] Take 2 grams of commercially available beef balls sample, chop them, add 5 ml of warm water and soak for 5 minutes. After filtering, take 1.5 ml of the filtrate and add it to borax test paper. Add 1 drop of curcumin solution, and reddish-brown spots will appear in the center of the drop. Then add 3-6 mol / L hydrochloric acid, and the test paper will turn green-black or blue-purple, indicating that borax has been added to the beef balls.

[0042] The present invention provides an embodiment for testing purchased noodle products:

[0043] In the testing of noodle products, 0.5 grams of flour sample was mixed with 8 milliliters of distilled water. After centrifugation, 2 milliliters of the supernatant was added to borax test paper. One drop of curcumin solution was added. There was no obvious change in the center of the drop. After adding 3-6 mol / L hydrochloric acid, the color of the test paper remained unchanged, indicating that the sample met the food safety standards.

[0044] The present invention provides an embodiment for on-site detection of sauce samples:

[0045] Weigh 2g of sauce and mix it with 5mL of distilled water. After oscillation extraction, filter. Take 1 drop of the filtrate and mix it with the curcumin solution on the test paper. After hot air drying, reddish-brown spots appear. After adding 6mol / L hydrochloric acid, the spots turn blue-purple, confirming that the sample contains borax. This method is suitable for rapid screening of fluid samples and the whole process takes only 10 minutes.

[0046] The present invention provides an embodiment to simplify the detection process of solid samples:

[0047] Grind the dried bean curd sheets directly into powder, take 2g, add 5mL of warm water and soak for 10 minutes, take 1 drop of the supernatant to react with curcumin, and if no color is shown after drying, it is judged to be borax-free. If color is shown and changes color after treatment with hydrochloric acid, the test needs to be repeated to eliminate the influence of interfering substances.

[0048] Through the above steps, curcumin solution is used as a color developer to generate a specific reddish-brown complex with boric acid under acidic conditions, 3-6 mol / L hydrochloric acid is added to enhance the color reaction, and a temperature-controlled hair dryer is used to accelerate the drying of the test paper, thereby shortening the detection time to 5-10 minutes. This effectively improves the sensitivity of the detection, enhances the anti-interference ability, and greatly improves the convenience of operation and the wide range of applications, thereby solving the problems of poor timeliness and complex operation of existing borax detection methods.

Claims

1. The color reaction detection method of the portable borax rapid detection kit is characterized in that: Includes: S1, processing the sample to be tested to prepare a sample solution to be tested; S2, take an appropriate amount of the solution to be tested and add it to the borax test paper in the kit; S3, adding a color developing reagent to the borax test paper; S5. Determine the borax content based on the color change and compare it with the standard color chart.

2. The color reaction detection method of the portable borax rapid detection kit according to claim 1, characterized in that: The color developing reagent is a curcumin solution composed of 0.35 mol / L curcumin + 3% hydrochloric acid. Curcumin combines with borax under acidic conditions to form a red complex for color development reaction with borax.

3. The color reaction detection method of the portable borax rapid detection kit according to claim 1, characterized in that: The sample treatment required weighing 2 g of sample and cutting it into pieces, then adding 5 mL of distilled water, shaking several times, and taking the supernatant as the sample solution after standing for 5 minutes.

4. The color reaction detection method of the portable borax rapid detection kit according to claim 1, characterized in that, The step S2 comprises: first taking a piece of borax test paper, then using a plastic straw to draw 1 drop of sample soaking liquid into the middle of the test paper, and the amount of sample soaking liquid added is 1-2 ml.

5. The color reaction detection method of the portable borax rapid detection kit according to claim 1, characterized in that: In step S3, one drop of curcumin solution is added to the same position where the sample soaking solution is added, and then the two solutions are evenly mixed.

6. The color reaction detection method of the portable borax rapid detection kit according to claim 5, characterized in that: After step S3 is completed, pinch a corner of the test paper with your hands or tweezers and blow dry it with hot air from a hair dryer.

7. The color reaction detection method of the portable borax rapid detection kit according to claim 6, characterized in that: If no reddish-brown spots appear on the test paper, stop the operation. If reddish-brown spots appear on the test paper, add one drop of 3-6 mol / L hydrochloric acid to the spots and observe the color change of the test paper.

8. The color reaction detection method of the portable borax rapid detection kit according to claim 7, characterized in that: After drying with a hair dryer, no red-brown spots appear in the center of the droplet, which means the sample does not contain borax. After adding 3-6mol / L hydrochloric acid, the test paper does not change color, which also means the sample does not contain borax. After drying with a hair dryer, red-brown spots appear in the center of the droplet, and after adding 3-6mol / L hydrochloric acid, the test paper turns green-black or blue-purple, which means the sample is mixed with borax.

9. The color reaction detection method of the portable borax rapid detection kit according to claim 8, characterized in that: The temperature of the hair dryer is controlled at 60-70°C, the wind speed is medium wind, and the test paper must be blown through before the result can be judged according to its color.

10. The color reaction detection method of the portable borax rapid detection kit according to claim 1, characterized in that: The standard colorimetric card includes multiple color scales from colorless to deep red, corresponding to borax concentrations of 0-100 mg / L respectively.

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