A method for artificially inducing functional components of Syringa ulmoides

Abstract

The present invention provides a method for artificially inducing functional components of pinnate lilac, relating to the field of botanical technology, comprising the following steps: S1, seedling adaptive cultivation, placing the pinnate lilac seedlings in an experimental greenhouse, using the experimental greenhouse to maintain 28-33°C, and growing under a natural light environment, for 8 weeks to obtain the initial adaptation of the pinnate lilac seedlings. The present invention accurately regulates the cultivation environment of the pinnate lilac seedlings, adopts the experimental greenhouse to implement adaptive cultivation for 8 weeks at a stable temperature of 28-33°C, and cooperates with natural light to enable the pinnate lilac seedlings to quickly adapt to the artificial environment, thereby laying a stable physiological foundation for subsequent lignan accumulation; at the same time, before planting, the substrate in the experimental greenhouse is configured to optimize the soil quality and microecology, providing excellent conditions for the subsequent growth of the plant; and then implementing a water stress treatment for 4 consecutive months to control the soil moisture content within the range of 15%-45%.

Description

Technical Field

[0001] The invention relates to the technical field of botany, and in particular to a method for artificially inducing functional components of Syringa pinnata. Background Art

[0002] Syringa syringae (called "mountain agarwood" in Mongolian medicine, named for its tendency to sink like water, indicating its higher specific gravity than common wood or branches) has been developed as a clinical medication for inhibiting gynecological inflammation, treating uterine prolapse, and combating myocardial ischemia. Its core functional components are polyphenols, cyclopentene (schizopentene) ether terpenes, and lignans, primarily derived from the perennial wild Syringa syringae. However, due to its long growth cycle, low natural regeneration capacity, and slow population growth in the wild, coupled with historically heavy harvesting, Syringa syringae was listed as a vulnerable to endangered species in the 1980s (see the China Red List of Precious and Endangered Plants), prohibiting its harvesting and making it difficult to obtain medicinal materials. Therefore, a method for artificially inducing the functional components of Syringa syringae is urgently needed. Summary of the Invention

[0003] The purpose of the present invention is to solve the shortcomings of the prior art and to propose a method for artificially inducing functional components of Syringa pinnata.

[0004] In order to achieve the above object, the present invention adopts the following technical solution: a method for artificially inducing functional components of Syringa ulmoides, comprising the following steps:

[0005] S1, seedling adaptation cultivation, placing the Syringa pinnata seedlings in an experimental greenhouse, using the experimental greenhouse to maintain 28-33 ° C, and growing them under natural light environment for 8 weeks to obtain the initial adaptation seedlings of Syringa pinnata;

[0006] S2, applying water stress to the Syringa pinnata seedlings in the experimental greenhouse for 4 months, stabilizing the moisture content at 15%-45%, and ending the stress after 4 months to obtain Syringa pinnata seedlings rich in lignans.

[0007] Preferably, in step S1, the substrate of the experimental greenhouse is made of vermiculite, garden soil, humus and perlite in a ratio of 1:3:2:1, and the moisture content of the substrate of the experimental greenhouse is controlled in the range of 15%-45%.

[0008] Compared to the existing technology, the present invention precisely regulates the cultivation environment of the syringa seedlings, adopts an experimental greenhouse at a stable temperature of 28-33 ° C for 8 weeks, and uses natural light to enable the syringa seedlings to quickly adapt to the artificial environment, laying a stable physiological foundation for subsequent lignan accumulation. At the same time, before planting, the substrate in the experimental greenhouse is configured to optimize the soil quality and microecology, providing excellent conditions for the subsequent growth of the plants. Then, a water stress treatment is implemented for 4 consecutive months to control the soil moisture content within the range of 15%-45%, so that the seedlings are in a mild stress state for a long time, stimulate the secondary metabolic pathways in the seedlings and increase the synthesis and accumulation of lignan active ingredients. As a result, the lignan content of the syringa seedlings is increased during growth, and the medicinal value is enhanced.

[0009] The technical solution of the present invention is further described in detail below through the accompanying drawings and embodiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0010] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0011] Figure 1 The present invention proposes a flow chart of a method for artificially inducing functional components of Syringa pinnata;

[0012] Figure 2 is a histogram of lignan compounds. DETAILED DESCRIPTION

[0013] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. All other embodiments obtained by ordinary technicians in this field based on the embodiments of the present invention without making any creative efforts shall fall within the scope of protection of the present invention.

[0014] Example 1, see Figure 1-2 As shown, a method for artificially inducing functional components of Syringa ulmoides L. comprises the following steps:

[0015] S1, seedling adaptation cultivation, two-year-old Syringa pinnata seedlings were placed in an experimental greenhouse, maintained at 33°C, and grown under natural light for 8 weeks to obtain initially adapted Syringa pinnata seedlings;

[0016] Inducing the growth of two-year-old Syringa pinnata seedlings is more effective in increasing the synthesis and accumulation of lignan bioactive ingredients. This is primarily due to the vigor and active metabolic systems of these seedlings at this stage. Two-year-old seedlings already possess relatively well-developed growth and defense systems, making them more sensitive to external induction signals and able to rapidly activate and enhance the activity of relevant enzymes, thereby promoting the lignan biosynthesis pathway. Compared to younger or mature plants, the induction effect is more pronounced, promoting the efficient accumulation of the target active ingredients.

[0017] S2, water stress was applied to two-year-old Syringa pinnata seedlings in an experimental greenhouse for 4 months, and the moisture content was stabilized at 17%. The stress was terminated after 4 months, and Syringa pinnata seedlings rich in lignans were obtained.

[0018] In step S1, the substrate of the experimental greenhouse is made of vermiculite, garden soil, humus and perlite in a ratio of 1:3:2:1, and the moisture content of the substrate of the experimental greenhouse is controlled at 15%.

[0019] Example 2 is the same as Example 1 in other respects, except that: S2, water stress is applied to the Syringa syringae seedlings in the experimental greenhouse for 4 months, and the moisture content is stabilized at 15%. After the stress lasts for 4 months, the water stress is ended to obtain Syringa syringae seedlings rich in lignans.

[0020] Example 3 is the same as Example 1 in other respects, except that: S2, water stress is applied to the Syringa syringae seedlings in the experimental greenhouse for 4 months, and the moisture content is stabilized at 30%. After the stress lasts for 4 months, the water stress is ended to obtain Syringa syringae seedlings rich in lignans.

[0021] Example 4 is the same as Example 1 except that: S2, the Syringa syringa seedlings are subjected to water stress for 4 months in the experimental greenhouse, and the moisture content is stabilized at 45%. After the stress lasts for 4 months, the water stress is ended to obtain Syringa syringa seedlings rich in lignans.

[0022] Comparative Example 1 is a wild Syringa ulmoides of the same age.

[0023] See Figure 2 As shown, the content of lignan compounds extracted from Eugenia caryophyllus obtained in Examples 1-4 and Comparative Example 1 is:

[0024] A: Monolignan has a protective effect against myocardial ischemia.

[0025] B: Monolignan glycoside is a compound formed by the glycosidic bond between monolignan and sugar molecules, which has antioxidant and anti-inflammatory biological activities.

[0026] C: Flavonol, a flavonol, is an antioxidant found in plants that helps protect against free radical damage and participates in a variety of biological processes.

[0027] D: Phenylethanol, as an aromatic alcohol, is often used in fragrances and cosmetics to provide a rose-like scent.

[0028] E: Polyphenol, whose main function is antioxidant, protecting cells from free radical damage.

[0029] F: Sesquiterpenoid, a sesquiterpenoid compound, whose main function is to participate in the plant's defense mechanism and has multiple biological activities, such as anti-inflammatory and antibacterial.

[0030] G: Secoiridoid, a secoiridoid terpene, has multiple biological activities such as anti-inflammatory, antioxidant and anti-tumor.

[0031] Figure 2 In the figure, treatment 1 corresponds to Example 2, treatment 2 corresponds to Example 3, treatment 3 corresponds to Example 4, treatment 4 corresponds to Example 1, and the wild same-age plants correspond to proportion 1; it can be seen from the figure that Example 1 is the best example, which can induce higher lignan chemical diversity and higher lignan content, which is significantly improved compared with normal wild planting.

Claims

1. A method for artificially inducing functional components of Syringa pinnata, characterized in that: The following steps are involved: S1, seedling adaptation cultivation, placing the Syringa pinnata seedlings in an experimental greenhouse, using the experimental greenhouse to maintain 28-33 ° C, and growing them under natural light environment for 8 weeks to obtain the initial adaptation seedlings of Syringa pinnata; S2, applying water stress to the Syringa pinnata seedlings in the experimental greenhouse for 4 months, stabilizing the moisture content at 15%-45%, and ending the stress after 4 months to obtain Syringa pinnata seedlings rich in lignans.

2. The method for artificially inducing functional components of Syringa pinnata according to claim 1, characterized in that: In step S1, the substrate of the experimental greenhouse is made of vermiculite, garden soil, humus and perlite in a ratio of 1:3:2:1, and the moisture content of the substrate of the experimental greenhouse is controlled in the range of 15%-45%.

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