Efficient cultivation technology for stropharia rugoso-annulata

Through the factory cultivation method of vertical segmentation of bacteria rods and layered soil covering combined with environmental regulation, the problems of long fungic cycle and dispersion of mushrooms in traditional large-sized caisson cultivation are solved, and efficient and concentrated large-sized large-sized caisson production is achieved.

CN120457948APending Publication Date: 2025-08-12TIANJIN ACAD OF AGRI SCI
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Patent Information

Application Number
CN202510706378.1
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-05-29
Publication Date
2025-08-12

AI Technical Summary

Technical Problem

In the traditional cultivation method of large ball caisson mushrooms, the long cycle of mycelium bacteria, the dispersed mushroom production time, and extensive environmental parameter control lead to poor synchrony of bud appearance, making it difficult to achieve centralized management and efficient production.

Method used

Factory-based cultivation methods are adopted for vertical segmentation of bacteria rods, layered soil cladding and precise environmental regulation, including vertical segmentation of bacteria rods, layered soil cladding structure, CO₂ concentration monitoring and programmatic ventilation management, and are combined with humidity and light control during the bacterial and budding periods.

Benefits of technology

The bacterial cycle has been shortened to 25-30 days, the mushroom production concentration has reached 85%, the bioconversion rate has been increased by 20-30%, the operation is standardized, and the resource utilization rate is high, which is suitable for cultivation in early spring facilities in the north.

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Abstract

The invention discloses an efficient cultivation method for stropharia rugoso-annulata. Factory production is achieved through mushroom stick vertical segmentation, a layered soil covering structure and precise environment regulation and control. The cylindrical mushroom sticks full of hyphae are vertically placed after being divided in half, so that a mushroom wall structure is formed, and the hypha contact surface is enlarged; a layered soil covering technology combining original soil bottom layer covering and a straw moisturizing layer is adopted, and staged humidity (the spawn running period is 60-70% and the squaring period is 50-60%) and illumination control (light shielding to 500-800 lux scattered light) are matched, so that the spawn running period is shortened to 25-30 days, and the fruiting concentration degree reaches 85% or above. COconcentration monitoring and programmed ventilation management are introduced, and the biological conversion rate is 65-75% and is increased by 20-30% compared with that of a traditional method. The method is especially suitable for early spring facility cultivation in northern areas, and has the characteristics of operation standardization, high resource utilization rate, convenience in harvesting management and the like.
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Description

Technical Field

[0001] The invention belongs to the technical field of edible fungus cultivation, and in particular relates to a factory-based efficient cultivation method of Stropharia rugosa based on vertical sowing of mushroom sticks and layered soil covering, which is particularly suitable for large-scale production in early spring in northern regions. Background Art

[0002] Stropharia rugosoannulata has attracted much attention due to its rich nutrition and wide range of cultivation materials. However, traditional cultivation methods have the following drawbacks: 1. The mycelium spawning cycle is as long as 45-50 days, and the fruiting time is scattered (the harvest period lasts 2-3 months), making centralized management difficult; 2. Conventional cultivation methods in facilities require pre-preparation of fermentation substrates, which is time-consuming and labor-intensive, and carries the risk of bacterial contamination. 3. The environmental parameters are controlled in a rough manner, the budding synchronization is poor, the harvesting period is relatively scattered, the mushroom batches are large, and the harvesting efficiency is low.

[0003] Therefore, there is an urgent need to develop a new cultivation method that can achieve rapid fungus growth, concentrated mushroom production, and high bioconversion rate. Summary of the Invention

[0004] A high-efficiency cultivation method for Stropharia rugosa is implemented according to the following steps: (1) Preparation of bacterial strains Select polyethylene bags with a ring size of 17 cm or above, and culture them in a dark environment at 22-25℃ and 65% humidity for 35-40 days until the mycelium fills the bag; after removing the bag, split the cylindrical mushroom stick in half along the axial direction into two semi-cylinders.

[0005] (2) Construction of cultivation beds Cultivation pits with a depth of 20-30cm and a width of 1-1.5m are dug in the greenhouse, with a distance of 30-40cm between adjacent pits; a breathable net with a pore size of 5mm can be laid at the bottom of the pit, and an atomizing spray pipe can be arranged above it.

[0006] (3) Vertical sowing When the ground temperature is ≥15℃ and the temperature in the greenhouse is ≥25℃ (preferably February-March in northern regions), place the semi-cylindrical mushroom sticks vertically at the bottom of the pit with the cut surface facing upwards, with a spacing of 5-10cm, to form a "mushroom wall" structure.

[0007] (4) Layered soil covering Bottom soil cover: backfill with original soil that has been exposed to sunlight and disinfected, completely covering the mushroom sticks and gaps, with a soil moisture content of 30-35%; Moisturizing layer: Lay a 3-5cm thick layer of straw or rice husk mixture and spray 0.1% lime water for disinfection.

[0008] (5) Environmental control Spawning period (from sowing to budding): Keep in a dark environment, with air humidity of 60-70% and temperature of 20-25℃; The CO2 concentration is tested every 3 days and controlled at ≤2000ppm.

[0009] Bud stage: Adjust to diffuse light (light intensity 500-800 lux) and humidity 50-60%; Ventilate twice a day, each time for 0.5-1 hour, and maintain a day-night temperature difference of 8-10℃.

[0010] Example (in a planting base in Xiqing District, Tianjin in 2025) Culture: Culture at 25°C for 38 days. When the mycelium fills a 17 cm bag, divide it into semi-cylinders. Constructing the cultivation bed: dig a 25cm deep and 1.2m wide cultivation pit and lay the misting pipe on the top; Vertical sowing: On March 30 (temperature in the greenhouse 26°C), place mushroom sticks vertically at intervals of 8 cm. Soil covering treatment: First cover with sterilized original soil (water content 32%), then lay a 3cm thick rice husk mixture layer; Environmental control: During the spawning period, the CO2 concentration should be controlled below 1800ppm. After the buds appear, ventilation should be carried out for 1 hour at 9:00 and 15:00 every day. Harvesting management: Buds appear 25 days after sowing, and the peak harvest period is 40 days.

Claims

1. A high-efficiency cultivation method for Stropharia rugosa, characterized in that The following steps are involved: (1) Mushroom stick processing: Split the cylindrical mushroom bag full of mycelium into two semi-cylinders; (2) Cultivation bed construction: dig a cultivation pit with a depth of 20-30 cm in the greenhouse and lay a breathable net at the bottom of the pit; (3) Vertical sowing: Place the semi-cylindrical mushroom sticks vertically at the bottom of the pit, with the cut surface facing outwards, with a spacing of 5-10 cm; (4) Layered soil covering: First cover the sterilized soil until the mushroom sticks are completely buried, and then lay a 3-5 cm layer of straw for moisture retention; (5) Staged control: Keep the germination period away from light and maintain a humidity of 60-70%. During the budding period, adjust to scattered light, humidity of 50-60% and implement programmed ventilation.

2. The method according to claim 1, characterized in that The bacteria in step (1) are cultured at 22-25° C. for 35-40 days, the diameter of the bacteria bag is 17 cm, and the height of the divided semi-cylinders is maintained at 15-17 cm.

3. The method according to claim 1, characterized in that In step (4), the covering soil material is sterilized by exposure to sunlight for 3 days, the moisture content of the original soil is 30-35%, and the straw moisture-retaining layer is a mixture of rice straw and rice husk in a mass ratio of 1:

2.

4. The method according to claim 1, characterized in that In step (5): During the germination period, the CO2 concentration is controlled at ≤2000ppm; during the budding period, ventilation is carried out twice a day for 0.5-1 hour each time, maintaining a day and night temperature difference of 8-10℃.

5. The method according to claim 1, characterized in that The intensity of the scattered light is 500-800 lux, which is achieved by the cooperation of the sunshade net and the fill light.

6. The method according to any one of claims 1 to 5, characterized in that It is suitable for areas above 35° north latitude. Buds will appear 25-30 days after sowing and the concentrated harvest period will begin 40-45 days after sowing.