Automatic zebra fish breeding system and method

Abstract

The invention discloses an automatic zebra fish breeding system and method, and relates to the technical field of breeding, the system comprises a breeding box, the breeding box comprises a box body and a box cover, a fish tank, a PH peristaltic pump, a conductivity peristaltic pump, a PH sensor, a conductivity sensor and a temperature sensor are arranged in the box body, and a heating fan is arranged on the side face of the box body; the computer is electrically connected with the PH peristaltic pump, the conductivity peristaltic pump, the PH sensor, the conductivity sensor, the temperature sensor and the heating fan, and the computer controls the PH peristaltic pump, the conductivity peristaltic pump and the heating fan to work, so that the PH value, the conductivity and the temperature in the box body are all in set states. The system is specially designed for the zebra fish, various environment requirements of the zebra fish are accurately controlled, and the automatic breeding effect of the zebra fish is greatly improved.

Description

Technical Field

[0001] The present application relates to the field of aquaculture technology, and in particular to an automated zebrafish aquaculture system and method. Background Art

[0002] As an important model organism, zebrafish has been widely used in many fields, including physiology, ecology, toxicology, breeding, and hatching. Therefore, in order to improve the efficiency and quality of zebrafish breeding, automated breeding systems have emerged.

[0003] Although there are many fish farming technologies available, zebrafish are different from other fish. They have very high requirements for the farming environment and some special water quality conditions, such as electrical conductivity. As a result, existing conventional fish farming technologies cannot be directly applied to zebrafish farming. Summary of the Invention

[0004] The embodiments of the present application provide a zebrafish automated breeding system and method to solve the problem that there is no automated breeding technology specifically for zebrafish in the prior art.

[0005] On the one hand, the embodiments of the present application provide an automated zebrafish breeding system, comprising:

[0006] A breeding box, which includes a box body and a box cover. A fish tank, a pH peristaltic pump, a conductivity peristaltic pump, a pH sensor, a conductivity sensor, and a temperature sensor are provided inside the box body. A communication interface and a heating fan are provided on the side of the box body. The fish tank is used to breed zebrafish. The pH peristaltic pump and the conductivity peristaltic pump are used to transport pH liquid and conductivity liquid into the box body, respectively. The pH sensor, the conductivity sensor, and the temperature sensor are used to collect the real-time pH value, real-time conductivity, and real-time temperature in the box body, respectively. The heating fan is used to transport heated air into the box body.

[0007] The computer is connected to the communication interface through a data cable, and the communication interface is electrically connected to the pH peristaltic pump, the conductivity peristaltic pump, the pH sensor, the conductivity sensor, the temperature sensor and the heating fan respectively. The computer compares the real-time pH value, the real-time conductivity and the real-time temperature with the corresponding control thresholds to control the operation of the pH peristaltic pump, the conductivity peristaltic pump and the heating fan so that the pH value, conductivity and temperature inside the box are all in the set state.

[0008] On the other hand, the present invention also provides an automated zebrafish farming method, comprising:

[0009] Start the water pump and UV lamp;

[0010] Set the control thresholds of pH value, conductivity and temperature, as well as the light control time on the computer;

[0011] Use a data cable to connect the computer and the breeding box;

[0012] The computer obtains the real-time pH value, real-time conductivity and real-time temperature in the breeding box, compares the real-time pH value, real-time conductivity and real-time temperature with the corresponding control thresholds, and controls the operation of the pH peristaltic pump, conductivity peristaltic pump and heating fan according to the comparison results;

[0013] The computer controls the operation of the lighting according to the light control time.

[0014] The automated zebrafish breeding system and method in this application have the following advantages:

[0015] 1. Improved production efficiency: Automated aquaculture systems can significantly reduce manpower input and achieve high yields. By precisely controlling the aquaculture environment, the system can support large-scale, multi-strain zebrafish farming, significantly improving production efficiency.

[0016] 2. Reduce operating costs: The automated breeding system introduces modern automatic control equipment, which can automatically monitor and adjust conditions such as light, pH value, conductivity and temperature, effectively reducing the waste of water, electricity and feed, thereby reducing operating costs.

[0017] 3. Improved aquaculture quality: The system can automatically adjust according to needs to fully guarantee the growth of zebrafish. At the same time, by automatically monitoring and adjusting water quality, it ensures that zebrafish grow and reproduce in a suitable environment, thereby improving aquaculture quality.

[0018] 4. Enhanced Experimental Stability: In scientific research, the automated zebrafish culture system provides a stable and controllable environment, enabling scientists to study zebrafish behavior, physiology, and ecology. Furthermore, automated analysis through online strategies and video processing allows for comprehensive assessment of zebrafish behavior, helping to improve experimental accuracy and stability. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without any creative work.

[0020] Figure 1 This is a schematic diagram of the three-dimensional structure of the breeding box in the zebrafish automated breeding system provided in an embodiment of the present application.

[0021] Figure 2 This is a left view of the breeding box provided in an embodiment of the present application.

[0022] Figure 3 This is a rear view of the breeding box provided in an embodiment of the present application.

[0023] Figure 4 This is a top view of the interior of the box provided in an embodiment of the present application.

[0024] Explanation of the accompanying figures: 1. Box body; 2. Box cover; 3. Lighting; 4. Camera; 5. Fish tank; 6. Communication interface; 7. Power interface; 8. Heating fan; 9. UV lamp; 10. pH peristaltic pump; 11. Conductivity peristaltic pump; 12. pH liquid storage box; 13. Conductivity liquid storage box; 14. Water tank; 15. pH sensor; 16. Conductivity sensor; 17. Temperature sensor. DETAILED DESCRIPTION

[0025] The following will be combined with the drawings in the embodiments of this application to clearly and completely describe the technical solutions in the embodiments of this application. Obviously, the embodiments described are only part of the embodiments of this application, not all of the embodiments. Based on the embodiments in this application, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of this application.

[0026] Figure 1-4 This is a schematic diagram of the structure of the breeding box in the zebrafish automated breeding system provided in the embodiment of the present application. The embodiment of the present application provides an automated zebrafish breeding system, which includes:

[0027] A breeding box, the breeding box includes a box body 1 and a box cover 2, a fish tank 5, a pH peristaltic pump 10, a conductivity peristaltic pump 11, a pH sensor 15, a conductivity sensor 16 and a temperature sensor 17 are provided inside the box body 1, and a communication interface 6 and a heating fan 8 are provided on the side of the box body 1. The fish tank 5 is used to breed zebrafish, the pH peristaltic pump 10 and the conductivity peristaltic pump 11 are respectively used to transport pH liquid and conductivity liquid into the box body 1, the pH sensor 15, the conductivity sensor 16 and the temperature sensor 17 are respectively used to collect the real-time pH value, real-time conductivity and real-time temperature in the box body 1, and the heating fan 8 is used to transport heated air to the inside of the box body 1;

[0028] The computer is connected to the communication interface 6 via a data cable. The communication interface 6 is electrically connected to the pH peristaltic pump 10, the conductivity peristaltic pump 11, the pH sensor 15, the conductivity sensor 16, the temperature sensor 17 and the heating fan 8 respectively. The computer compares the real-time pH value, the real-time conductivity and the real-time temperature with the corresponding control thresholds to control the operation of the pH peristaltic pump 10, the conductivity peristaltic pump 11 and the heating fan 8 so that the pH value, conductivity and temperature inside the box 1 are all in the set state.

[0029] Exemplarily, the box body 1 is a hollow cubic structure with an opening at the top, and the box cover 2 is connected to the opening at the top of the box body 1 by a hinge. When the box cover 2 is closed, the internal environment of the box body 1 can be isolated from the outside world to prevent the external environment from affecting the breeding environment inside the box body 1.

[0030] The fish tank 5 is also a hollow cubic structure with an open top, but the size of the fish tank 5 is much smaller than that of the box 1, and multiple fish tanks 5 are placed in the box 1. The multiple fish tanks 5 are arranged in multiple rows and columns in the box 1, and each fish tank 5 can raise one or several zebrafish.

[0031] Furthermore, the housing 1 is formed by splicing together multiple aluminum alloy plates, with adjacent aluminum alloy plates being fixedly connected using screws. The overall dimensions of the fish tank 5 are 13 cm long, 11.5 cm wide, and 12.5 cm high, and the fish tank 5 is made of acrylic plates with a thickness of 2-3 mm and bonded with non-toxic fish tank glue.

[0032] The communication interface 6 can specifically be a USB (Universal Serial Bus) interface, which is connected to the USB interface of a computer via a USB data cable to enable the computer to automatically control the breeding box.

[0033] The heating fan 8 is an integrated structure of a fan and a heating unit, and is installed at the air inlet provided on the side of the housing 1. Under computer control, the heating fan 8 heats the air outside the housing 1 to a set temperature and ultimately delivers it to the interior of the housing 1. To ensure that the air input by the heating fan 8 can properly enter the housing 1, an air outlet is also provided on the other side of the housing 1. Furthermore, both the air inlet and outlet of the housing 1 are required to be equipped with one-way valves to ensure that air can only enter through the air inlet and exit through the air outlet.

[0034] In a possible embodiment, a water tank 14 is provided inside the housing 1 , and the water in the fish tank 5 overflows and flows into the water tank 14 .

[0035] Exemplarily, the water tank 14 is made of PP board, and a water pump and a filtering device are provided in the water tank 14. The filtering device is used to filter the water in the water tank 14. The water inlet of the water pump is connected to the water outlet of the filtering device, and the water outlet of the water pump is connected to the fish tank 5.

[0036] The pH sensor 15, the conductivity sensor 16 and the temperature sensor 17 all adopt a waterproof packaging structure, and the three sensors are all installed on the inner bottom surface of the water tank 14 to respectively collect the real-time pH value, real-time conductivity and real-time temperature of the water collected in the water tank 14. Specifically, the temperature measurement range of the temperature sensor 17 in the embodiment of the present application is -50°C-110°C, the temperature measurement accuracy is ±0.2°C, and the temperature control accuracy of the heating fan 8 is ±0.1°C. When the real-time temperature collected by the temperature sensor 17 is lower than the set temperature, preferably 28°C, the computer will control the heating fan 8 to start to heat the air in the box body 1. When the real-time temperature reaches 28°C, the heating fan 8 will stop working.

[0037] In the filtration device, a physical filtration layer, an activated carbon filtration layer and a reverse osmosis membrane filtration layer are arranged in sequence in the direction of water flow. The physical filtration layer can be made of filter mesh, filter cotton, etc., and this multi-layer filtration structure can effectively purify harmful substances such as mud, rust, bacteria, suspended matter, algae and large molecular organic matter in the water, providing a good breeding environment for zebrafish.

[0038] Furthermore, a horizontal partition is provided inside the box body 1 , and a water hole is provided on the partition. The fish tank 5 is provided on the top surface of the partition, and the water tank 14 is provided on the inner bottom surface of the box body 1 , and the water hole is located directly above the water tank 14 .

[0039] Specifically, the partition may also be made of aluminum alloy, and is fixed to the inner side of the box body 1 by welding or screw connection.

[0040] The water pump continuously delivers filtered water to the fish tank 5, and a portion of the water will overflow from the top of the fish tank 5. The water will return to the water tank 14 through the water hole, thereby achieving water circulation, filtration and utilization.

[0041] Furthermore, the pH sensor 15, the conductivity sensor 16 and the temperature sensor 17 are all disposed inside the water tank 14 to detect the state of the water in the water tank 14. Accordingly, the pH peristaltic pump 10 and the conductivity peristaltic pump 11 are both disposed inside the housing 1 near the water tank 14.

[0042] Furthermore, the housing 1 is provided with a pH liquid reservoir 12 and a conductivity liquid reservoir 13, which are connected to the pH peristaltic pump 10 and the conductivity peristaltic pump 11, respectively. The pH liquid reservoir 12 and the conductivity liquid reservoir 13 are used to store sodium bicarbonate solution and sodium chloride solution, respectively. When the real-time pH value is lower than 7, the pH peristaltic pump 10 will start under computer control and add sodium bicarbonate solution to the water in the water tank 14 at a certain flow rate. When the real-time pH value reaches 7, the pH peristaltic pump 10 will stop. When the real-time conductivity is lower than 500, the conductivity peristaltic pump 11 will start under computer control and add sodium chloride solution to the water in the water tank 14 at a certain flow rate. When the real-time conductivity reaches 500, the conductivity peristaltic pump 11 will stop.

[0043] Furthermore, an ultraviolet lamp 9 is screwed onto the inner bottom surface of the housing 1. The ultraviolet light generated by the ultraviolet lamp 9 irradiates the water in the water tank 14, which can achieve a sterilization effect. The input voltage range of the ultraviolet lamp 9 is 200-240VAC.

[0044] In a possible embodiment, there are multiple fish tanks 5, which are arranged in multiple rows and columns in the box body 1. The box cover 2 is provided with multiple cameras 4 on the side facing the box body 1. The cameras 4 correspond one-to-one to the fish tanks 5, and the cameras 4 are electrically connected to the communication interface 6.

[0045] For example, the camera 4 is fixed to the tank cover 2 by screws, has an operating temperature of -20°C to 70°C, a maximum resolution of 4556*3496, an operating voltage of 5V, and a fixed focal length range of 140mm. At the same time, the distance between the fish tank 5 and the camera 4 is 140mm.

[0046] The video captured by the camera 4 can be transmitted to the display screen of the computer through the communication interface 6, so that the management personnel can view the status of the zebrafish in each fish tank 5 in the breeding box in real time.

[0047] Furthermore, a light 3 is provided on the side of the lid 2 facing the tank body 1. The light 3 is electrically connected to the communication interface 6. The computer controls the operation of the light 3 according to the set light control time. The light 3 is an LED (light emitting diode) light strip. Since the light 3 is also installed on the lid 2, the distance between the light 3 and the fish tank 5 is also 140 mm.

[0048] In the embodiment of the present application, the light control time is set to 10 hours during the day and 14 hours at night. The computer will control the lighting lamp 3 to turn on and off according to this time length, providing the zebrafish with a lighting environment that simulates the natural state.

[0049] In a possible embodiment, a power interface 7 is provided on the outer surface of the box body 1, and the power interface 7 is used to plug in the power cord. The power interface 7 is also electrically connected to the pH peristaltic pump 10, the conductivity peristaltic pump 11 and the heating fan 8 respectively.

[0050] For example, the voltage input to the power interface 7 is 220VAC, and the power ends of the lighting lamp 3, ultraviolet lamp 9, pH peristaltic pump 10, conductivity peristaltic pump 11, heating fan 8 and water pump are respectively connected to the power interface 7. When the power is connected, the switch on the outer surface of the operating box 1 can control the start of the ultraviolet lamp 9 and the water pump, while the lighting lamp 3, pH peristaltic pump 10, conductivity peristaltic pump 11 and heating fan 8 are started under the control of the computer. At the same time, the camera 4, pH sensor 15, conductivity sensor 16 and temperature sensor 17 are all powered by the communication interface 6.

[0051] The present invention also provides an automated zebrafish farming method, which comprises the following steps:

[0052] Start the water pump and UV lamp;

[0053] Set the control thresholds of pH value, conductivity and temperature, as well as the light control time on the computer;

[0054] Use a data cable to connect the computer and the breeding box;

[0055] The computer obtains the real-time pH value, real-time conductivity and real-time temperature in the breeding box, compares the real-time pH value, real-time conductivity and real-time temperature with the corresponding control thresholds, and controls the operation of the pH peristaltic pump 10, the conductivity peristaltic pump 11 and the heating fan 8 according to the comparison results;

[0056] The computer controls the operation of the lighting lamp 3 according to the light control time.

[0057] Although the preferred embodiments of the present application have been described, those skilled in the art may make additional changes and modifications to these embodiments once they have learned the basic creative concept. Therefore, the appended claims are intended to be interpreted as including the preferred embodiments and all changes and modifications that fall within the scope of the present application.

[0058] Obviously, those skilled in the art may make various changes and modifications to this application without departing from the spirit and scope of this application. Thus, if these modifications and variations of this application fall within the scope of the claims of this application and their equivalents, this application is intended to include these modifications and variations.

Claims

1. The zebrafish automated breeding system is characterized by: include: A breeding box, comprising a box body (1) and a box cover (2), wherein a fish tank (5), a pH peristaltic pump (10), a conductivity peristaltic pump (11), a pH sensor (15), a conductivity sensor (16) and a temperature sensor (17) are provided inside the box body (1), a communication interface (6) and a heating fan (8) are provided on the side of the box body (1), the fish tank (5) is used for breeding zebrafish, the pH peristaltic pump (10) and the conductivity peristaltic pump (11) are used for respectively transporting pH liquid and conductivity liquid into the box body (1), the pH sensor (15), the conductivity sensor (16) and the temperature sensor (17) are used for respectively collecting real-time pH value, real-time conductivity and real-time temperature in the box body (1), and the heating fan (8) is used for transporting heated air into the inside of the box body (1); A computer is connected to the communication interface (6) via a data line. The communication interface (6) is electrically connected to the pH peristaltic pump (10), the conductivity peristaltic pump (11), the pH sensor (15), the conductivity sensor (16), the temperature sensor (17) and the heating fan (8), respectively. The computer compares the real-time pH value, the real-time conductivity and the real-time temperature with corresponding control thresholds to control the operation of the pH peristaltic pump (10), the conductivity peristaltic pump (11) and the heating fan (8), so that the pH value, conductivity and temperature inside the box (1) are all in a set state.

2. The zebrafish automated breeding system according to claim 1, characterized in that: A water tank (14) is provided inside the box body (1), and the water in the fish tank (5) overflows and flows into the water tank (14).

3. The zebrafish automated breeding system according to claim 2, characterized in that: A water pump and a filter device are provided in the water tank (14). The filter device is used to filter the water in the water tank (14). The water inlet of the water pump is connected to the water outlet of the filter device, and the water outlet of the water pump is connected to the fish tank (5).

4. The zebrafish automated breeding system according to claim 2, characterized in that: A horizontal partition is provided inside the box body (1), and a water hole is provided on the partition. The fish tank (5) is provided on the top surface of the partition. The water tank (14) is provided on the inner bottom surface of the box body (1), and the water hole is located directly above the water tank (14).

5. The zebrafish automated breeding system according to claim 4, characterized in that: An ultraviolet lamp (9) is provided on the inner bottom surface of the box (1).

6. The zebrafish automated breeding system according to claim 1, characterized in that: There are multiple fish tanks (5), and the multiple fish tanks (5) are arranged in multiple rows and columns in the box body (1). The box cover (2) is provided with multiple cameras (4) on the side facing the box body (1). The cameras (4) correspond to the fish tanks (5) one by one, and the cameras (4) are electrically connected to the communication interface (6).

7. The zebrafish automated breeding system according to claim 1, characterized in that: The box cover (2) is provided with a lighting lamp (3) on the side facing the box body (1); the lighting lamp (3) is electrically connected to the communication interface (6); and the computer controls the operation of the lighting lamp (3) according to a set illumination control time.

8. The zebrafish automated breeding system according to claim 1, characterized in that: A pH liquid storage box (12) and a conductivity liquid storage box (13) are also provided inside the box (1), and the pH liquid storage box (12) and the conductivity liquid storage box (13) are connected to the pH peristaltic pump (10) and the conductivity peristaltic pump (11) respectively.

9. The zebrafish automated breeding system according to claim 1, characterized in that: A power interface (7) is provided on the outer surface of the box body (1), and the power interface (7) is used to plug in a power cord. The power interface (7) is also electrically connected to the pH peristaltic pump (10), the conductivity peristaltic pump (11) and the heating fan (8) respectively.

10. The method for applying the zebrafish automated breeding system according to any one of claims 1 to 9, characterized in that: include: Start the water pump and UV lamp; Set the control thresholds of pH value, conductivity and temperature, as well as the light control time on the computer; Use a data cable to connect the computer and the breeding box; The computer obtains the real-time pH value, real-time conductivity and real-time temperature in the breeding box, compares the real-time pH value, the real-time conductivity and the real-time temperature with the corresponding control threshold values, and controls the operation of the pH peristaltic pump (10), the conductivity peristaltic pump (11) and the heating fan (8) according to the comparison results; The computer controls the operation of the lighting lamp (3) according to the illumination control time.

Images