Plant growth regulator for inhibiting plant germination and application thereof

Abstract

The invention belongs to the technical field of plant growth regulators, and particularly relates to a plant growth regulator for inhibiting plant germination and application thereof, the plant growth regulator for inhibiting plant germination is obtained by compounding S-abscisic acid and dihydrogibberellin; in the plant growth regulator for inhibiting plant germination, the molar ratio of dihydrogibberellin to S-abscisic acid is (0.2-2): (0.1-1). By combining dihydrogibberellin with S-abscisic acid, the proportion of endogenous hormone gibberellin and abscisic acid in wheat grains can be effectively reduced, the activity of alpha-amylase is reduced, the content of soluble sugar is reduced, the dormancy capacity of the wheat grains is enhanced, and the germination rate of the wheat grains in the mature period is remarkably reduced.

Description

Technical Field

[0001] The present invention belongs to the technical field of plant growth regulators, and in particular relates to a plant growth regulator for inhibiting plant germination and an application thereof. Background Art

[0002] wheat( Triticum aestivum L. ) is widely cultivated as a staple food source due to its exceptional adaptability. If wheat encounters continuous rainy weather before harvest at maturity, the kernels will sprout directly on the ear. This sprouting can lead to a sharp decline in wheat yield and quality. The starch and protein-degrading enzymes produced by the kernels during germination deplete storage substances like starch and protein, resulting in a decrease in thousand-kernel weight and negatively impacting wheat processing quality, such as significant reductions in gluten content, sedimentation value, and dough rheological properties. Swelling kernels, kernels with cracked seed coats, kernels with exposed white areas, and kernels with extended radicles or germs resulting from wheat ear sprouting are all considered imperfect kernels. This not only seriously affects kernel morphology, but also increases the proportion of imperfect kernels, leading to a decline in wheat grade. Sprouted kernels consume some of the seed's nutrients, preventing them from germinating or growing into normal seedlings. Furthermore, the seed extracts contain toxic substances and mold that can affect the germination of otherwise normal seeds, severely impacting seed quality.

[0003] During the wheat harvest season, rainy and humid weather can reduce seed dormancy, often leading to spike sprouting. Current methods for combating spike sprouting primarily rely on leveraging wheat's inherent resistance to spike sprouting, spraying the plant growth regulator S-inducer, or implementing field management measures. S-inducer has been widely used in recent years, but its effectiveness alone in inhibiting spike sprouting is not ideal. Summary of the Invention

[0004] In order to solve the above technical problems, the present invention provides a plant growth regulator for inhibiting wheat ear germination before harvest, so as to reduce the occurrence of the problem of wheat grains germinating on the ears due to the inability to harvest normally due to continuous rainy weather during the wheat maturity period.

[0005] To achieve the above objectives, the technical solutions of the present invention are specifically as follows.

[0006] A first aspect of the present invention provides a plant growth regulator for inhibiting wheat spike germination, wherein the plant growth regulator for inhibiting plant germination is a compound of S-absorbent and dihydrogibberellin to regulate the ratio of endogenous hormones in plant grains; In the plant growth regulator for inhibiting plant germination, the molar ratio of dihydrogibberellin to S-absorbent is 0.2-2:0.1-1.

[0007] The present invention combines dihydrogibberellin with S-inducer in a molar ratio of 0.2-2:0.1-1, thereby effectively reducing the ratio of endogenous hormones gibberellin to abscisic acid in wheat grains, reducing α-amylase activity, reducing soluble sugar content, enhancing the dormancy ability of wheat grains, and thus significantly reducing the germination rate of wheat grains at maturity.

[0008] In another preferred embodiment, the plant growth regulator is a liquid preparation and a solid preparation.

[0009] In another preferred embodiment, the liquid preparation is any one of an aqueous suspension, an aqueous emulsion, a microemulsion, and a soluble liquid.

[0010] In another preferred embodiment, the solid preparation is any one of a soluble powder, a water-dispersible granule, an effervescent tablet, and a soluble tablet.

[0011] The second aspect of the present invention provides the use of the plant growth regulator for inhibiting plant germination in inhibiting wheat ear germination.

[0012] The third aspect of the present invention provides the use of the plant growth regulator for inhibiting plant germination in improving the dormancy ability of wheat grains.

[0013] The fourth aspect of the present invention provides the use of the plant growth regulator for inhibiting plant germination in regulating endogenous hormones in wheat grains.

[0014] In another preferred embodiment, the endogenous hormones are gibberellins and abscisic acid.

[0015] A fifth aspect of the present invention provides a method for applying the plant growth regulator for inhibiting plant germination, comprising the following steps: According to a fourth aspect of the present invention, after the wheat flowers, the diluted solution of the plant growth regulator for inhibiting plant germination and the diluted solution of its preparation are sprayed on the leaves and ears of the wheat.

[0016] In another preferred embodiment, the spraying amount of the plant sprouting inhibitor is 15 L / mu to 30 L / mu.

[0017] Compared with the prior art, the present invention has the following beneficial effects: The plant growth regulator used in the present invention is compounded with dihydrogibberellin and S-inducible in a molar ratio of 0.2-2:0.1-1, which can reduce the ratio of endogenous hormones gibberellin to abscisic acid in wheat grains, enhance wheat grain dormancy, and significantly reduce the spike germination rate.

[0018] The plant growth regulator for inhibiting wheat ear germination used in the present invention can reduce the α-amylase activity of wheat grains, inhibit the conversion of starch into soluble sugars, and reduce the loss of wheat yield and quality caused by ear germination. The plant growth regulator for inhibiting wheat ear germination provided by the present invention has a small usage amount, a significant effect, no health hazards to the human body, a simple operation process, low cost, and is suitable for large-scale promotion and application. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 The figures show the effects of different concentrations of dihydrogibberellic acid treatment on the germination rate of the whole ear of detached wheat ears harvested 35 days after flowering on the 7th day of simulated rainfall; in the figure, (a) shows the effects of different concentrations of dihydrogibberellic acid treatment on the germination rate of the whole ear of detached ears of Jimai 22 harvested 35 days after flowering on the 7th day of simulated rainfall, in the figure, a1 represents the three parallels of the plant growth regulator 1 treatment group, a2 represents the three parallels of the plant growth regulator 2 treatment group, a3 represents the three parallels of the plant growth regulator 3 treatment group, a4 represents the three parallels of the control group, and a5 represents the three parallels of the positive control group; (b) shows the effects of different concentrations of dihydrogibberellic acid treatment on the germination rate of the whole ear of detached ears of Yannong 1212 harvested 35 days after flowering on the 7th day of simulated rainfall, in the figure, b1 represents the three parallels of the plant growth regulator 1 treatment group, b2 represents the three parallels of the plant growth regulator 2 treatment group, b3 represents the three parallels of the plant growth regulator 3 treatment group, b4 represents the three parallels of the control group, and b5 represents the three parallels of the positive control group.

[0020] Figure 2 The figures show the effects of different concentrations of S-absorbent on the germination rate of detached ears of wheat harvested 35 days after flowering on the 7th day of simulated rainfall; in the figure, (a) shows the effects of different concentrations of S-absorbent on the germination rate of detached ears of Jimai 22 harvested 35 days after flowering on the 7th day of simulated rainfall, in the figure, a1 represents three parallels of the plant growth regulator 4 treatment group, a2 represents three parallels of the plant growth regulator 5 treatment group, a3 represents three parallels of the plant growth regulator 6 treatment group, a4 represents three parallels of the control group, and a5 represents three parallels of the positive control group; (b) shows the effects of different concentrations of S-absorbent on the germination rate of detached ears of Yannong 1212 harvested 35 days after flowering on the 7th day of simulated rainfall, in the figure, b1 represents three parallels of the plant growth regulator 4 treatment group, b2 represents three parallels of the plant growth regulator 5 treatment group, b3 represents three parallels of the plant growth regulator 6 treatment group, b4 represents three parallels of the control group, and b5 represents three parallels of the positive control group.

[0021] Figure 3This is a graph showing the effect of the combined treatment of dihydrogibberellin and S-inducible on the germination rate of the whole ear of wheat detached ears harvested 35 days after anthesis and on the 7th day of simulated rainfall; in the figure, (a) shows the effect of the combined treatment of dihydrogibberellin and S-inducible on the germination rate of the whole ear of Jimai 22 detached ears harvested 35 days after anthesis and on the 7th day of simulated rainfall, in the figure, a1 represents three parallel samples of the group treated with plant growth regulator 7, a2 represents three parallel samples of the group treated with plant growth regulator 8, a3 represents three parallel samples of the group treated with plant growth regulator 9, and a4 a represents three parallels of the control group, a5 represents three parallels of the positive control group; (b) shows the effect of the combined treatment of dihydrogibberellin and S-inducing agent on the germination rate of the whole ear of Tobacco Farmer 1212 harvested 35 days after flowering on the 7th day of simulated rainfall. In the figure, b1 represents three parallels of the group treated with plant growth regulator 7, b2 represents three parallels of the group treated with plant growth regulator 8, b3 represents three parallels of the group treated with plant growth regulator 9, b4 represents three parallels of the control group, and b5 represents three parallels of the positive control group.

[0022] Figure 4 The results of the effect of the combined treatment of dihydrogibberellic acid and S-inducing agent on the α-amylase activity of detached wheat ears harvested 35 days after anthesis and subjected to simulated rainfall for 7 days are shown in the figure; in the figure, (a) is the result of α-amylase activity in the grains of detached ears of Jimai 22, and (b) is the result of α-amylase activity in the grains of detached ears of Yannong 1212; in the figure, CK represents the water control assistant group, A3 represents the plant growth regulator 2 treatment group, B3 represents the plant growth regulator 6 treatment group, C1 represents the plant growth regulator 7 treatment group, C2 represents the plant growth regulator 8 treatment group, and C3 represents the plant growth regulator 9 treatment group.

[0023] Figure 5 The results show the effect of the combined treatment of dihydrogibberellic acid and S-inducing agent on the soluble sugar content of detached wheat ears harvested 35 days after flowering and subjected to simulated rainfall for 7 days; in the figure, (a) is the result graph of the soluble sugar content of the grains of the detached ears of Jimai 22, and (b) is the result graph of the soluble sugar content of the grains of the detached ears of Yannong 1212. In the figure, CK represents the water control treatment group, A3 represents the plant growth regulator 2 treatment group, B3 represents the plant growth regulator 6 treatment group, C1 represents the plant growth regulator 7 treatment group, C2 represents the plant growth regulator 8 treatment group, and C3 represents the plant growth regulator 9 treatment group.

[0024] Figure 6The results of the effect of the combined treatment of dihydrogibberellic acid and S-elicitor on the content of plant hormones GA and ABA in detached wheat ears harvested 35 days after flowering and subjected to simulated rainfall for 7 days; in the figure, (a) is the result of the ratio of plant hormones GA and ABA content in grains of detached ears of Jimai 22, and (b) is the result of the ratio of plant hormones GA and ABA content in grains of detached ears of Yannong 1212; in the figure, CK represents the water control treatment group, A3 represents the plant growth regulator 2 treatment group, B3 represents the plant growth regulator 6 treatment group, C1 represents the plant growth regulator 7 treatment group, C2 represents the plant growth regulator 8 treatment group, and C3 represents the plant growth regulator 9 treatment group. DETAILED DESCRIPTION

[0025] The present invention will be described in detail below with reference to the accompanying drawings and specific examples, but they should not be construed as limiting the present invention. Unless otherwise specified, the technical means used in the following examples are conventional means well known to those skilled in the art, and the materials, reagents, etc. used in the following examples, unless otherwise specified, can be obtained from commercial sources.

[0026] The tested wheat varieties were Jimai 22, recorded as JM22, Yannong 1212, recorded as YN1212, gibberellin, recorded as GA, and abscisic acid, recorded as ABA.

[0027] Dihydrogibberellic acid was provided by the Chemical Control Center of the College of Agriculture, China Agricultural University, and S-inducible and 2 wt% gibberellic acid were provided by Sichuan Guoguang Agrochemical Co., Ltd.

[0028] Example 1: Screening of active ingredients of plant growth regulators that inhibit wheat ear germination.

[0029] Experiments were conducted with different concentrations of dihydrogibberellic acid, S-absorbent, and a combination of dihydrogibberellic acid and S-absorbent, and water was used as a control to obtain different plant growth regulators that inhibit plant germination. The specific components are shown in Table 1.

[0030] Table 1 Components of different plant germination inhibitors Note: Clean water can be any one of tap water, well water, and deionized water. Deionized water is used in this embodiment.

[0031] The experiment adopted a split-plot design. Each treatment was repeated three times, and the area of each plot was 4m 2Plant growth regulators listed in Table 1 were sprayed on wheat leaves and ears at a rate of 30 L / mu 30 days after anthesis. Forty ears were collected from each plot 35 days after anthesis and brought indoors. Ten ears were threshed, and 250 seeds were divided into five groups for seed germination testing to determine the germination rate. The remaining 30 ears were subjected to simulated rainfall for 2 hours daily for five days. On the seventh day, ten ears were randomly selected to calculate the whole-ear germination rate and bud length.

[0032] Grain germination rate formula: ; Formula for whole ear germination rate: ; Bud growth formula: .

[0033] The results are shown in Table 2 and Figures 1 to 3 As shown, different plant growth regulators that inhibit plant germination have different degrees of inhibitory effects on ear germination compared with the blank group.

[0034] Table 2 Inhibitory effects of different treatments on wheat spike germination Note: Different lowercase letters after the values indicate differences at the 0.05 significance level.

[0035] Combine Figures 1 to 3 From Table 2, it can be seen that at the same concentration, the inhibitory effect of dihydrogibberellic acid is higher than that of S-absorbent, and the effect of using dihydrogibberellic acid and S-absorbent together is higher than that of using them alone, and the effect is best with the increase of the concentration of dihydrogibberellic acid and S-absorbent, that is, the combined treatment of 1.2mmol / L dihydrogibberellic acid and 0.3mmol / L S-absorbent has the most significant effect on the two wheat varieties. Compared with the blank group, the grain germination rate of Jimai 22 decreased by 25.6%, the whole ear germination rate decreased by 17.07%, and the bud length decreased by 1.76cm. The grain germination rate of Yannong 1212 decreased by 27.6%, the whole ear germination rate decreased by 20.99%, and the bud length decreased by 2.06cm. This shows that the combination of dihydrogibberellic acid and S-absorbent has a significant inhibitory effect on wheat ear germination.

[0036] Example 2: Related physiological and biochemical indicators of plant growth regulators.

[0037] Ten ears of rice were sprayed with equal amounts of plant growth regulators 3, 6, 7, 8, and 9, using water as a blank control. Soluble sugar content, α-amylase activity, and the levels of the plant hormones gibberellins and abscisic acid were measured. α-amylase and soluble sugar content were measured using kits provided by Beijing Shenggong Box, while gibberellins and abscisic acid were measured using enzyme-linked immunosorbent assays.

[0038] The results are as follows Figures 4 to 6 As shown in the figure, it can be seen that plant growth regulators 7, 8 and 9 have the effect of reducing the activity of α-amylase and inhibiting the hydrolysis of starch into soluble sugars, and soluble sugars are the energy substances required for wheat grain germination. The germination rate of wheat grains is reduced due to the restriction of the energy substances required for germination; the endogenous hormone GA in wheat grains can promote seed germination, and ABA inhibits seed germination. When the ratio of gibberellin to abscisic acid becomes higher, the dormancy of seeds is enhanced and the germination ability is reduced. The plant growth regulators 7, 8 and 9 in the present invention can inhibit the synthesis of endogenous GA in wheat grains and promote the production of endogenous ABA. The ratio of GA to ABA is significantly lower than that of the clear water control group, indicating that plant growth regulators 7, 8 and 9 can inhibit the germination of wheat ears.

[0039] Example 3: Liquid formulation of plant growth regulator for inhibiting plant germination.

[0040] A liquid preparation was prepared using the plant growth regulator 8 for plant germination in Example 1 as an example. The specific formula is shown in Table 2. The components in Table 2 were mixed to obtain an aqueous emulsion.

[0041] Table 3 Liquid preparation ratio Note: The emulsifier OP-10 was purchased from Guangzhou Haosheng Chemical Co., Ltd., and the defoaming agent SAG630 was purchased from Guangdong Zhonglianban Fine Chemical Co., Ltd.

[0042] Example 4: Preparation of a solid formulation of a plant growth regulator for inhibiting wheat ear germination.

[0043] A solid preparation was prepared using the plant growth regulator 8 for plant germination in Example 1 as an example, and the specific formula is shown in Table 3. The components in Table 3 were mixed, and soluble tablets were obtained by granulation and tableting.

[0044] Table 4 Solid preparation ratio Note: Defoaming agent B290 was purchased from Guangdong Zhonglianban Fine Chemical Co., Ltd.

[0045] Although the preferred embodiments of the present invention have been described, those skilled in the art may make additional changes and modifications to these embodiments once they have learned the basic creative concept. Therefore, the appended claims are intended to be interpreted as including the preferred embodiments and all changes and modifications that fall within the scope of the present invention.

[0046] Obviously, those skilled in the art may make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if such modifications and variations of the present invention fall within the scope of the claims and their equivalents, the present invention is intended to include such modifications and variations.

Claims

1. A plant growth regulator for inhibiting plant germination, characterized in that: The plant growth regulator for inhibiting plant germination is obtained by compounding S-antibiotic and dihydrogibberellin; In the plant growth regulator for inhibiting plant germination, the molar ratio of dihydrogibberellin to S-absorbent is 0.2-2:0.1-1.

2. The plant growth regulator for inhibiting plant germination according to claim 1, characterized in that The plant growth regulator is a liquid preparation or a solid preparation.

3. The plant growth regulator for inhibiting plant germination according to claim 1, characterized in that The liquid preparation is any one of an aqueous suspension, an aqueous emulsion, a microemulsion, and a soluble liquid.

4. The plant growth regulator for inhibiting plant germination according to claim 1, characterized in that The solid preparation is any one of soluble powder, water-dispersible granules, effervescent tablets, and soluble tablets.

5. Use of the plant growth regulator for inhibiting plant germination according to any one of claims 1 to 4 in inhibiting wheat ear germination.

6. Use of the plant growth regulator for inhibiting plant germination according to any one of claims 1 to 4 in improving the dormancy ability of wheat grains.

7. Use of the plant growth regulator for inhibiting plant germination according to any one of claims 1 to 4 in regulating endogenous hormones in wheat grains.

8. Use of the plant growth regulator for inhibiting plant germination according to claim 7 in regulating endogenous hormones in wheat grains, characterized in that: The endogenous hormones are gibberellins and abscisic acid.

9. A method for applying the plant growth regulator for inhibiting plant germination according to any one of claims 1 to 4, characterized in that: The following steps are involved: 25 to 30 days after the wheat flowers, the plant growth regulator for inhibiting plant germination is sprayed on the leaves and ears of the wheat.

10. The method for applying a plant growth regulator for inhibiting plant germination according to claim 9, characterized in that: The spraying amount of the plant growth regulator for inhibiting plant germination is 15L / mu to 30L / mu.

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