Application of norzelamic aldehyde in preparing products for preventing and controlling Phytophthora

Abstract

This invention belongs to the field of modern agricultural technology and specifically relates to the use of demethylzeylasteral (DZL) in the preparation of products for controlling Phytophthora spp. This invention demonstrates the inhibitory effect of pentacyclic triterpenoid demethylzeylasteral on the activity of Phytophthora capsici and other crop fungi. Demethylzeylasteral can inhibit the fungus, sporangium release, zoospore germination, and pathogenicity, demonstrating its anti-Phytophthora spp. activity. Therefore, the compounds provided by this invention can be used to prepare small molecule inhibitors of the growth, development, and pathogenicity of Phytophthora spp., demonstrating their potential as potential anti-Phytophthora spp. drugs and possessing promising development and application value.

Description

Technical Field

[0001] The invention belongs to the technical field of modern agriculture, and particularly relates to application of norzelamic aldehyde in preparing products for preventing and controlling Phytophthora. Background Art

[0002] Phytophthora pathogens cause numerous crop diseases, including potato late blight, soybean blight, litchi downy mildew, pepper blight, durian root rot, rubber monsoon leaf drop, and pineapple heart rot. Among these, Phytophthora capsici, Phytophthora palmi, Phytophthora parasiticus, and Phytophthora tobacco have a wide range of host plants, infecting both underground and aboveground parts of host plants, including roots, stems, leaves, and fruit. Due to their rapid and prolonged infection rate, insensitivity to many fungicides, and high genetic diversity, Phytophthora is one of the most difficult diseases to control.

[0003] Phytophthora fungi typically survive in the soil or on diseased plant debris as thick-walled spores or oospores. Under favorable conditions, they germinate and form hyphae and asexual sporangia, forming the primary source of infection. A frosty mildew layer forms on the surface of lesions on host plants, along with numerous asexual sporangia, which are then spread by water and air currents and subsequently infect. Under favorable conditions, the sporangia produce numerous zoospores, which, after a period of movement, recognize and infect the host plant, causing symptoms such as necrosis. Currently, crop disease control relies primarily on chemical agents such as metalaxyl, dimethomorph, cymoxanil, azoxystrobin, and propamocarb. However, the long-term, frequent use of single chemical agents has led to a decline in their efficacy in recent years, resulting in the development of resistance in many pathogens. Therefore, there is a need to develop more effective, environmentally friendly, and safer drugs against Phytophthora fungi.

[0004] Demethylzeramaldehyde is an effective monomeric compound extracted from Tripterygium wilfordii. Existing research only discloses its therapeutic effects in human diseases. For example, the Chinese patent "Potential Application of Demethylzeramaldehyde in Combating Mycobacterium Infection" (Publication No. CN112294825A) discloses that demethylzeramaldehyde inhibits 3-dehydroquinate dehydratase in Mycobacterium tuberculosis, making it a potential anti-TB drug. However, it provides no technical insights into plant diseases. To date, there are no reports on the use of demethylzeramaldehyde for controlling plant Phytophthora. Summary of the Invention

[0005] The first aspect of the present invention aims to provide a use of norzelamic aldehyde in the preparation of a product that inhibits the activity of Phytophthora.

[0006] The second aspect of the present invention aims to provide a use of norzelamic aldehyde in the preparation of products for preventing and treating crop blight.

[0007] The third aspect of the present invention aims to provide a method for preventing and controlling Phytophthora.

[0008] The fourth aspect of the present invention aims to provide a fungicide.

[0009] In order to achieve the above-mentioned purpose of the present invention, the technical solution adopted by the present invention is:

[0010] The first aspect of the present invention provides the use of norzelamic aldehyde in preparing a product for inhibiting the activity of Phytophthora.

[0011] In some embodiments of the present invention, the chemical formula of the demethylzeylasteral (DZL) is C 29 H 36 O6, CAS No. 107316-88-1, structural formula is shown in formula (I):

[0012] Formula (I).

[0013] In some embodiments of the present invention, the activity of inhibiting Phytophthora includes any one or more of the following:

[0014] (1) Inhibit the radial growth of Phytophthora hyphae;

[0015] (2) Inhibit the production of sporangia and release of zoospores of Phytophthora;

[0016] (3) Inhibit the germination of zoospores of Phytophthora;

[0017] (4) Inhibit the pathogenicity and virulence of Phytophthora.

[0018] In some embodiments of the present invention, the Phytophthora includes but is not limited to: Phytophthora capsici ( P. capsici ), Lychee Downy Phytophthora ( P. litchi ), Phytophthora nicotianae ( P.nicotianae )、Phytophthora palmitoides( P.palmivora )、Phytophthora sojae( P.sojae ), Phytophthora vulgaris ( P. vignae ), Phytophthora truncatula ( P. colocasiae )、Phytophthora cryptogenomica( P.cryptogea ) and Phytophthora diggellii ( P. drechsleri ).

[0019] In some embodiments of the present invention, the demethylzeramaldehyde comprises demethylzeramaldehyde or a pharmaceutically acceptable salt form thereof.

[0020] In some embodiments of the present invention, the pharmaceutically acceptable salts include acid addition salts and base addition salts.

[0021] In some embodiments of the present invention, "pharmaceutically acceptable acid addition salts" refers to salts that retain the biological effectiveness and properties of the free bases, are not biologically or otherwise undesirable, and are formed with inorganic acids such as, but not limited to, hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid, phosphoric acid, and the like, and organic acids such as, but not limited to, acetic acid, adipic acid, alginic acid, ascorbic acid, aspartic acid, benzenesulfonic acid, benzoic acid, camphoric acid, camphor-10sulfonic acid, capric acid, hexanoic acid, caprylic acid, carbonic acid, cinnamic acid, citric acid, cyclamic acid, dodecylsulfuric acid, ethane-1,2-disulfonic acid, ethanesulfonic acid, 2-hydroxyethanesulfonic acid, fumaric acid, galactaric acid, gentisic acid, gluconic acid, glucuronic acid, glutamic acid, glutaric acid, 2-hydroxy-1,2-disulfonic acid, ethanesulfonic ... Oxoglutaric acid, glycerophosphate, glycolic acid, hippuric acid, isobutyric acid, lactic acid, lactobionic acid, lauric acid, maleic acid, malic acid, malonic acid, mandelic acid, methanesulfonic acid, etc.

[0022] In some embodiments of the present invention, "pharmaceutically acceptable base addition salts" refer to salts that retain the biological effectiveness and properties of the free acid and are not biologically or otherwise undesirable. These salts are prepared by the addition of inorganic or organic bases to the free acid. Salts derived from inorganic bases include, but are not limited to, sodium salts, potassium salts, lithium salts, ammonium salts, calcium salts, magnesium salts, iron salts, zinc salts, copper salts, manganese salts, aluminum salts, and the like. Preferred inorganic salts are ammonium salts, sodium salts, potassium salts, calcium salts, and magnesium salts. Salts derived from organic bases include, but are not limited to, salts of primary, secondary, and tertiary amines, substituted amines (including naturally occurring substituted amines), cyclic amines, and basic ion exchange resins, such as ammonia, isopropylamine, trimethylamine, diethylamine, triethylamine, tripropylamine, diethanolamine, ethanolamine, dicyclohexylamine, lysine, arginine, histidine, caffeine, ethylenediamine, glucosamine, methylglucamine, theobromine, triethanolamine, tromethamine, purine, piperazine, piperidine, N-ethylpiperidine, polyamine resins, and the like. Particularly preferred organic bases are isopropylamine, diethylamine, ethanolamine, trimethylamine, dicyclohexylamine, choline, and caffeine. In some embodiments of the invention,

[0023] In some embodiments of the invention, the product comprises a pesticide.

[0024] In some embodiments of the present invention, the pesticide includes a pharmaceutically acceptable excipient.

[0025] In some embodiments of the present invention, the pharmaceutically acceptable excipients include at least one of solvents, propellants, solubilizers, cosolvents, emulsifiers, colorants, adhesives, disintegrants, fillers, lubricants, wetting agents, osmotic pressure regulators, stabilizers, glidants, preservatives, suspending agents, fragrances, anti-adhesives, penetration enhancers, pH regulators, buffers, plasticizers, surfactants, foaming agents, defoaming agents, thickeners, inclusion agents, humectants, absorbents, diluents, flocculants and deflocculating agents, and filter aids.

[0026] In some embodiments of the present invention, the pharmaceutically acceptable excipients are generally recognized for this purpose and are used as inactive ingredients in pharmaceutical preparations. A compilation of pharmaceutically acceptable excipients can be found in reference books such as the Handbook of Pharmaceutical Excipients (2nd edition, edited by A. Wade and P.J. Weller; published by the American Pharmaceutical Association, Washington and The Pharmaceutical Gess, London, 1994); and the Pharmacopoeia of the People's Republic of China - List of Pharmaceutical Excipients.

[0027] In some embodiments of the present invention, the dosage form of the pesticide includes one of a solid preparation, a liquid preparation, and a volatile preparation.

[0028] In some embodiments of the present invention, the dosage form of the pesticide can be found in national standards such as "Pesticide Formulation Names and Codes GB / T19378-2017".

[0029] In some embodiments of the present invention, the use concentration of norzelamic aldehyde is 1-200 μg / mL.

[0030] In some embodiments of the present invention, when used to inhibit the radial growth of Phytophthora mycelia, the concentration of demethylzearalenaldehyde is 10-60 μg / mL.

[0031] In some embodiments of the present invention, when used to inhibit the production of sporangia and release of zoospores of Phytophthora, the concentration of demethylzelaminaldehyde is 2-50 μg / mL.

[0032] In some embodiments of the present invention, when used to inhibit the germination of zoospores of Phytophthora, the concentration of demethylzeramaldehyde is 1-10 μg / mL.

[0033] In some embodiments of the present invention, when used to inhibit the pathogenicity and virulence of Phytophthora, the concentration of demethylzelaminaldehyde is 5-50 μg / mL.

[0034] The second aspect of the present invention provides the use of norzelamic aldehyde in the preparation of products for preventing and treating crop blight.

[0035] In some embodiments of the present invention, the prevention and treatment include prophylaxis and treatment.

[0036] In some embodiments of the present invention, the crop phytophthora diseases include but are not limited to: pepper phytophthora, soybean phytophthora, cowpea phytophthora, litchi downy mildew, pepper blight, durian root rot, rubber monsoon leaf drop, pineapple heart rot, tobacco phytophthora, palm phytophthora, taro phytophthora, and melon phytophthora.

[0037] In some embodiments of the present invention, the Phytophthora includes but is not limited to: Phytophthora capsici ( P. capsici ), Lychee Downy Phytophthora ( P. litchi ), Phytophthora nicotianae ( P.nicotianae )、Phytophthora palmitoides( P.palmivora )、Phytophthora sojae( P.sojae ), Phytophthora vulgaris ( P. vignae ), Phytophthora truncatula ( P. colocasiae )、Phytophthora cryptogenomica( P. cryptogea ) and Phytophthora diggellii ( P. drechsleri ).

[0038] In some embodiments of the present invention, the sites of occurrence of crop blight include but are not limited to: fruits, leaves, flowers, branches, and roots.

[0039] The third aspect of the present invention provides a method for preventing and treating phytophthora, comprising the following steps:

[0040] Plants were treated with norzelamic aldehyde.

[0041] In some embodiments of the present invention, the prevention and treatment include prophylaxis and treatment.

[0042] In some embodiments of the present invention, the plants include but are not limited to pepper, litchi, tobacco, palm, soybean, cowpea, taro, melon, etc.

[0043] In some embodiments of the present invention, the plant parts include but are not limited to fruits, leaves, flowers, branches, and roots.

[0044] In some embodiments of the present invention, the use concentration of norzelamic aldehyde is 10-100 μg / mL.

[0045] The beneficial effects of the present invention are:

[0046] The present invention discovers for the first time that demethyl zearalenaldehyde has the effect of preventing and controlling crop blight, and has significant antibacterial activity against pepper Phytophthora, litchi downy mildew, tobacco Phytophthora, palm Phytophthora, taro Phytophthora, cowpea Phytophthora, digger Phytophthora, and soybean Phytophthora. In addition, demethyl zearalenaldehyde significantly reduces the area of ​​lesions on pepper leaves inoculated with pepper Phytophthora. Experiments confirm the preventive effect of demethyl zearalenaldehyde on pepper blight, thereby achieving the purpose of the present invention and having great significance for preventing and controlling crop diseases.

[0047] Demethylzearalenaldehyde is a natural plant extract used in the food industry. It is safe, environmentally friendly, sustainable, and not prone to resistance. It has great development and application value in the prevention and control of post-harvest diseases of precious fruits.

[0048] Experiments of the present invention have shown that demethylzearalenaldehyde can be used for the prevention and treatment of diseases caused by pepper phytophthora, litchi downy mildew, tobacco phytophthora, palm phytophthora, taro phytophthora, and cowpea phytophthora. It can be used alone or in combination with other antibacterial agents to enhance the antibacterial effect or reduce side effects. It is green and safe, and plays a role in preventing and treating phytophthora diseases and preserving fruits and vegetables. BRIEF DESCRIPTION OF THE DRAWINGS

[0049] The present invention will be further described below with reference to the accompanying drawings and embodiments, in which:

[0050] Figure 1 The present invention discloses the inhibition of the radial growth of mycelium of pepper Phytophthora capsici LT1534 by demethyl zearalenaldehyde and the effect on the inhibition of radial growth of mycelium of pepper Phytophthora capsici in different regions; wherein, A: inhibition of radial growth of pepper Phytophthora capsici LT1534 by demethyl zearalenaldehyde; B: statistical analysis of the effect of demethyl zearalenaldehyde on the growth diameter of LT1534; C: statistical analysis of the growth inhibition rate of LT1534 by demethyl zearalenaldehyde; D: effect of demethyl zearalenaldehyde on the radial growth inhibition of pepper Phytophthora capsici in different regions; E: statistical analysis of the growth inhibition rate of pepper in different regions by demethyl zearalenaldehyde.

[0051] Figure 2 The present invention shows the effect of nor-zeramaldehyde on the inhibition of sporangium release of Phytophthora capsici; wherein, A: the effect of nor-zeramaldehyde on the sporangium release of Phytophthora capsici; B: the statistical analysis of nor-zeramaldehyde on the sporangium release of Phytophthora capsici; C: the statistical analysis of the half-maximal inhibition of nor-zeramaldehyde on the sporangium release of Phytophthora capsici.

[0052] Figure 3 The present invention shows the effect of nor-zeramaldehyde on the inhibition of zoospore germination of Phytophthora capsici; wherein, A: the effect of nor-zeramaldehyde on the zoospore germination of Phytophthora capsici; B: the statistical analysis of nor-zeramaldehyde on the zoospore germination of Phytophthora capsici; C: the statistical analysis of the half-maximal inhibition of nor-zeramaldehyde on the zoospore germination of Phytophthora capsici.

[0053] Figure 4 The present invention shows the effects of nor-zeramaldehyde on the pathogenicity and virulence of pepper Phytophthora capsici; wherein, A: the effect of nor-zeramaldehyde on the pathogenicity of pepper leaves infected by Phytophthora capsici; B: statistical analysis of the effect of nor-zeramaldehyde on the lesion area of ​​pepper leaves infected by Phytophthora capsici.

[0054] Figure 5 The inhibition graph of the present invention on the radial growth of mycelia of different Phytophthora species is shown in FIG; wherein, A: the inhibition of the present invention on the radial growth of different Phytophthora species by demethyl zara wood aldehyde P. litchi ), Phytophthora nicotianae ( P.nicotianae )、Phytophthora palmitoides( P.palmivora ), Phytophthora vulgaris ( P. vignae ), Phytophthora truncatula ( P. colocasiae ), and Phytophthora diggingii ( P. drechsleri ) Effect of radial growth; B: Statistical analysis of the inhibition rate of demethyl zarathoxylum aldehyde on the growth of different Phytophthora fungi.

[0055] Figure 6 This is a graph showing the inhibition of nor-zeramaldehyde on the production of sporangia of litchi peronophytum; wherein, A: the effect of nor-zeramaldehyde on the number of sporangia produced by litchi peronophytum; B: statistical analysis of the effect of nor-zeramaldehyde on the number of sporangia produced by litchi peronophytum.

[0056] Figure 7 The present invention shows the effects of nor-zeramaldehyde on the pathogenicity and virulence of litchi downy mildew; wherein, A: the effect of nor-zeramaldehyde on litchi downy mildew infection of litchi fruit; B: statistical analysis of nor-zeramaldehyde on the lesion area of ​​litchi fruit infected by litchi downy mildew. DETAILED DESCRIPTION

[0057] The following will clearly and completely describe the concept and technical effects of the present invention in conjunction with the embodiments to fully understand the purpose, features and effects of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, other embodiments obtained by those skilled in the art without creative work are all within the scope of protection of the present invention.

[0058] Unless otherwise specified, the experimental methods used in the following examples are conventional methods.

[0059] Unless otherwise specified, the materials and reagents used in the following examples can be obtained from commercial sources.

[0060] Example 1 Effect of demethylzeramaldehyde on the antibacterial activity of Phytophthora capsici

[0061] The test pathogens: Phytophthora capsici LT1534, HNPC, 700407, WYS12-11, AH10, and ZY1 were all isolated from pepper and preserved in our laboratory.

[0062] Main reagents: Demethylzelamidin was purchased from Sichuan Jingcui Tiancheng Pharmaceutical Technology Co., Ltd. with a purity of 95-99%.

[0063] V8 agar medium was used for the cultivation and spore production of Phytophthora capsici.

[0064] The mycelial growth rate method was used to determine the inhibitory effect of different concentrations of demethylzeramaldehyde on the mycelial growth of pathogens. The specific procedures were as follows: 1) DMSO was used as a solvent to dissolve demethylzeramaldehyde to prepare a stock solution; 2) a certain amount of the prepared demethylzeramaldehyde stock solution was accurately added to the V8 medium that was cooled to about 50°C and mixed evenly, so that the final concentrations of demethylzeramaldehyde in the medium reached 2, 4, 6, 10, 20, 30, 40, and 60 μg / mL, respectively. Four replicates were set for each concentration treatment, and the same volume of solvent at the half-inhibitory concentration was added as a control. 20 mL / dish of culture medium was poured into a 9 cm diameter culture dish; 3) A sterile yellow (1-200 μL) pipette tip was used to poke a bacterial cake (d=5 mm) of the same age from the edge of the colony of activated pepper fungus cultured on V8, and the bacterial cake was inoculated into the center of the V8 medium plate containing different concentrations of demethylzeramaldehyde and placed at 25 The cells were cultured in the dark in a constant temperature incubator at ℃, and their diameters were observed and measured regularly and photographed.

[0065] See the results Figure 1 , and demethylzeramaldehyde had a significant antibacterial effect on pepper phytophthora. When the concentration of demethylzeramaldehyde reached 29.16 μg / mL, the inhibition rate against pepper phytophthora LT1534 reached 50% ( Figure 1 AC); When the concentration of demethylzelaminaldehyde reached 35 μg / mL, it had a significant inhibitory effect on pepper phytophthora in several different regions, with an inhibition rate of 42.78%-55.18% ( Figure 1 middle DE).

[0066] Example 2 Effect of demethylzeramaldehyde on the antibacterial activity of different types of spores of Phytophthora capsici

[0067] Preparation of pathogen sporangium suspension: Pepper Phytophthora capsici was inoculated on V8 medium for 4-5 days and transferred to a light incubator to induce sporangia for 3 days. Sporangia of pepper were washed with different concentrations of demethylzelaminaldehyde (1, 3, 5, 7 and 9 μg / mL) and the sporangia were collected by filtration and the concentration was adjusted to 1*10 5 / mL, treated at 4 ℃ for 20 min to induce sporangium release, and the sporangium release was observed after standing at room temperature for 0, 15, 30 min and 1, 2 h respectively;

[0068] Zoospore germination: An appropriate amount of sterile water was added to the conidia-producing plate and treated at 4°C for 20 min to induce sporangium release. The zoospores were filtered and treated with 1, 3, 5, 7, and 9 μg / mL of demethylzelamidin, respectively. The zoospore germination was observed after standing at room temperature for 0, 15, 30 min and 1, 2 h, respectively.

[0069] See the results Figure 2 and Figure 3 , norzelaminaldehyde has a significant inhibitory effect on the release of sporangia and zoospore germination of pepper phytophthora. When the concentration of norzelaminaldehyde reaches above 1 μg / mL and the treatment lasts for more than 15 minutes, it has a significant inhibitory effect on the release of sporangia and zoospore germination of pepper phytophthora, while DMSO treatment has no inhibitory effect ( Figure 2 Middle AB and Figure 3 Effects of norzelaminaldehyde on sporangium release and zoospore germination of LT1534 50 1.3 and 3.39 μg / mL respectively ( Figure 2 Middle C and Figure 3 The results showed that norzelaminaldehyde significantly inhibited the sporangium release and zoospore germination of Phytophthora capsici.

[0070] Example 3 Effects of norzelamic aldehyde on the pathogenicity and virulence of Phytophthora capsici

[0071] Test materials: The test strain was Capsicum LT1534, and the test plant was Capsicum HNCB226.

[0072] The leaves of living pepper seedlings were treated with 2, 8, 16, 27 and 35 μg / mL of demethylzelaminaldehyde solutions, respectively. Freshly activated cultured Phytophthora capsici cakes of the same diameter were inoculated onto the surfaces of pepper leaves treated with different concentrations of the drug. The inoculated pepper plants were placed in a moisturizing box and sprayed with moisture. They were then cultured at room temperature. Leaves treated with DMSO and 35 μg / mL of demethylzelaminaldehyde served as blank controls, and leaves inoculated with Phytophthora capsici treated with DMSO served as a positive control. The leaf lesion area was observed and recorded.

[0073] See the results Figure 4 , norzelaminaldehyde has a good preventive effect on pepper phytophthora, and with the increase of norzelaminaldehyde concentration, the preventive effect is improved, and the area of ​​pepper phytophthora infection is reduced ( Figure 4 Middle AB).

[0074] Example 4 Inhibitory Effects of Demethyl Zeramaldehyde on Different Phytophthora

[0075] Test pathogens: P.litchi, P.nicotianae, P.palmivora, P.vignae, P.colocasiae, P.drechsleri, P.cryptogea Isolated from tropical crops in China, and P. capsici、P. sojae Stored by this laboratory.

[0076] V8 agar medium was used for the cultivation and spore production of Phytophthora capsici. The mycelial growth rate method was used to determine the inhibitory effect of different concentrations of demethylzeramaldehyde on the mycelial growth of the pathogen. The specific procedures were as follows: 1) demethylzeramaldehyde was dissolved in DMSO to prepare a stock solution; 2) a certain amount of the prepared demethylzeramaldehyde stock solution was accurately added to the melted V8 medium cooled to about 50°C and mixed evenly to make the final concentrations of demethylzeramaldehyde in the medium reach 2, 10, 20, 40, and 60 μg / mL, respectively. Four replicates were set for each concentration treatment. The same volume of solvent at the half-inhibitory concentration was added as a control. 20 mL / dish of culture medium was poured into 9 cm diameter culture dishes; 3) A sterile yellow (1-200 μL) pipette tip was used to poke a bacterial cake (d = 5 mm) of the same age at the edge of the activated pepper P. capsici colony cultured on V8. The bacterial cake was then inoculated into the center of a V8 medium plate containing different concentrations of demethylzeramaldehyde and incubated in a constant temperature incubator at 25°C in the dark. The diameter of the cake was regularly observed, measured, and photographed.

[0077] Table 1

[0078]

[0079] See the results Figure 5 As shown in Table 1, norzelaminaldehyde has a significant antibacterial effect on Phytophthora. P.litchi, P.nicotianae, P.palmivora, P.vignae, P.colocasiae, P.drechsleri, P. capsici、P.cryptogea and P.sojae EC 50 The concentrations were 26.27, 36.32, 40.07, 31.38, 25.53, 36.86, 30.54, 53.98 and 60 μg / mL (Table 1), and they all had different degrees of antibacterial effects on different Phytophthora species ( Figure 5 Middle AB).

[0080] Example 5 Inhibitory Effect of Demethylzeramaldehyde on Sporangia Production of Peronophytum litchii

[0081] Test pathogen: Lychee Downy Phytophthora P. litchi Strain SHS3.

[0082] Litchi downy mildew was inoculated onto V8 medium containing 2, 10, 20, and 40 μg / mL demethylzelaminaldehyde for 4-5 days, and then induced to produce sporangium. After the sporulation was completed, the number of sporangia was observed, photographed, recorded, and statistically analyzed.

[0083] See the results Figure 6 , norzelaminaldehyde had a significant inhibitory effect on the sporangium production of Peronophytum litchii at concentrations above 2 μg / mL, and the inhibitory effect became more obvious with increasing concentrations, while DMSO treatment had no significant inhibitory effect ( Figure 6 The results showed that norzelaminaldehyde had a significant inhibitory effect on the sporangium production of Peronophytum litchii.

[0084] Example 6: Effect of norzelamaldehyde on the prevention of litchi downy mildew

[0085] Test Materials: Litchi cultivar "Fei Zi Xiao" was purchased from the market. Individual lychee fruits were manually separated, and healthy lychee fruits of consistent size, shape, and maturity were selected. These fruits were sterilized in 75% alcohol solution for 3 minutes, rinsed three times with sterile water, air-dried, and then grouped and processed.

[0086] Specific procedures: 1) Air-dried litchi fruits were treated with 10, 20, 40, 60, and 80 μg / mL of norzelamic acid for 8 minutes. The water-treated group served as a blank control, and the DMSO-treated group served as a solvent control. Dimethomorph (80% active ingredient) commercially available from Shandong Bainongsida Biotechnology Co., Ltd. was used as a control. Five replicates were set for each concentration treatment, and a total of 40 fruits were inoculated. After air-drying at room temperature, 5 mm diameter cakes of Peronophytum litchii were inoculated onto the surface of the treated litchi fruits. The fruits treated with each concentration were placed in plastic containers, sprayed with moisture, and placed in a 25°C incubator for treatment. The fruit were observed and photographed 72, 96, and 120 hours after treatment to record the incidence and lesion area.

[0087] See the results Figure 7 Demethylzelaminaldehyde has a significant inhibitory effect on litchi downy mildew, and the control effect increases with the increase of concentration. When the concentration reaches 10-20 μg / mL, it has a significant control effect after 72 hours of treatment; when the concentration reaches above 40 μg / mL, it has a significant control effect after 96-120 hours of treatment; when the concentration reaches 80 μg / mL, it has a control effect equivalent to or even better than commercial drugs ( Figure 7 Middle AB).

[0088] While the embodiments of the present invention have been described in detail above with reference to the accompanying drawings, the present invention is not limited to the embodiments described above. Various modifications may be made within the scope of knowledge possessed by a person skilled in the art without departing from the spirit of the present invention. Furthermore, the embodiments of the present invention and the features thereof may be combined with one another unless there is a conflict.

Claims

1. Application of norzelamic aldehyde in the preparation of products for inhibiting the activity of Phytophthora; The phytophthora is Phytophthora capsici, Phytophthora litchii, Phytophthora tobacco, Phytophthora palmi, Phytophthora soybean, Phytophthora cowpea, Phytophthora taro, Phytophthora cryptogescens or Phytophthora digellii.

2. The use according to claim 1, characterized in that: The activity of inhibiting Phytophthora includes any one or more of the following: (1) Inhibit the radial growth of Phytophthora hyphae; (2) Inhibit the production of sporangia and release of zoospores of Phytophthora; (3) Inhibit the germination of zoospores of Phytophthora; (4) Inhibit the pathogenicity and virulence of Phytophthora.

3. The use according to claim 1, characterized in that: The demethylzeramaldehyde includes demethylzeramaldehyde or a pharmaceutically acceptable salt thereof.

4. The use according to claim 1, characterized in that: The products include pesticides.

5. The use according to claim 4, characterized in that: The pesticide includes pharmaceutically acceptable excipients.

6. The use according to claim 5, characterized in that: The pharmaceutically acceptable excipients include at least one of a colorant, a binder, a disintegrant, a lubricant, an osmotic pressure regulator, a stabilizer, a preservative, a suspending agent, a fragrance, an anti-adhesive agent, a penetration enhancer, a pH regulator, a buffer, a plasticizer, a surfactant, a foaming agent, a defoaming agent, a thickener, an inclusion agent, a humectant, an absorbent, a diluent, a flocculant and a deflocculating agent, and a filter aid.

7. The use according to claim 6, characterized in that: The dosage form of the pesticide includes one of a solid preparation, a liquid preparation and a volatile preparation.

8. The use according to claim 1, characterized in that: The use concentration of the demethylzeramaldehyde is 1-80 μg / mL.

9. A method for preventing and controlling phytophthora, comprising the following steps: Treating plants with norzelamic aldehyde; The pathogen of the phytophthora disease is Phytophthora capsici, Phytophthora litchii, Phytophthora tobacco, Phytophthora palmi, Phytophthora soybean, Phytophthora cowpea, Phytophthora taro, Phytophthora cryptogescens or Phytophthora digellii.

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