Preparation technology of glycolysis postbiotic polypeptide

Abstract

The invention discloses a glycolysis biogen polypeptide preparation technology, and belongs to the field of biological products, the glycolysis biogen polypeptide preparation technology comprises the following steps: blanching and stirring a cereal substrate and water with a temperature of more than or equal to 95 DEG C according to a ratio of 1: 0.8-1.1, cooking and curing at a temperature of more than or equal to 100 DEG C for more than or equal to 30 min, inoculating with a material 1 and 0.2-0.4% of lactobacillus plantarum, stirring and mixing at a speed of 50-80 r / min, and carrying out sealed fermentation for 24-36 h at a thickness of 20-25 cm and a temperature of 30-35 DEG C, crushing the fermented material until the particle size is less than or equal to 5mm and more than or equal to 100 DEG C, cooking for more than or equal to 10 minutes, performing secondary fermentation by using the material 1 and 1.5-3% of rhizopus, fermenting for less than or equal to 30 hours at the material thickness of less than or equal to 15cm, hot air at the temperature of 30-35 DEG C and the fan rotating speed of 70-100 revolutions / min, grinding into thick liquid by using a colloid mill with the material-liquid ratio of 1: (5-6), performing spray drying, and performing quantitative packaging. According to the process, cereal substrates which are wide in source and low in cost are adopted, conditions of all the steps are clear and easy to control, the product is rich in metabiotics and polypeptide, the synergistic effect on regulating the physiological function of the human body is higher compared with a single enzymolysis peptide product, health can be comprehensively promoted, and large-scale industrial production is facilitated.

Description

Technical Field

[0001] The present invention relates to the field of biological products, and specifically discloses a process for preparing a post-fermentation biotic polypeptide. Background Art

[0002] In today's food and biopharmaceutical sectors, the development and preparation of functional ingredients remains a key research focus. Peptides, with their diverse bioactivities, such as antioxidant, immunomodulatory, and blood pressure-lowering properties, hold broad application prospects in the nutrition, health, and pharmaceutical industries, attracting the attention of numerous researchers and businesses.

[0003] Currently, the predominant method for producing peptides on the market is enzymatic hydrolysis. Enzymatic hydrolysis involves the hydrolysis of substrates using specific enzymes to produce peptide products. However, this traditional enzymatic hydrolysis process has significant limitations. Enzymatic hydrolysis merely utilizes the catalytic action of enzymes to break down macromolecules into small peptides; the entire process does not involve microbial fermentation and metabolism. Consequently, enzymatic peptide products lack the production of microbial metabolites, or postbiotics. Postbiotics, substances produced by microorganisms during their growth and metabolism, such as short-chain fatty acids, exopolysaccharides, and bacteriocins, possess diverse physiological activities. They play important roles in regulating intestinal microbial balance, enhancing immunity, and improving intestinal barrier function, exerting significant and positive impacts on human health. Therefore, enzymatic peptide products lacking postbiotics lack functional diversity and fail to meet consumer demand for products with more comprehensive health benefits. To address the limitations of traditional enzymatic hydrolysis, the development of a preparation process that can simultaneously produce both peptides and postbiotics is urgently needed.

[0004] Therefore, it is necessary to invent a process for preparing post-fermentation polypeptides to solve the above problems. Summary of the Invention

[0005] The purpose of the present invention is to provide a process for preparing a glycolytic postbiotic polypeptide.

[0006] In order to achieve the above object, the present invention provides the following technical solutions: A process for preparing postbiotic polypeptides after fermentation, comprising the following steps: Step 1: Blanch and stir the cereal substrate at a material:water ratio of 1:0.8-1.1; Step 2: Steam the blanched and stirred material until cooked, with the cooking temperature being ≥100°C and the cooking time being ≥30 min; Step 3: Inoculation of matured materials: Add 2-4‰ of fermentation bacteria (Lactobacillus plantarum) to the treated materials according to step 2, stirring at 50-80 rpm until the mixture is evenly mixed. Step 4: Loading and fermentation: the material thickness is 20-25cm, the temperature is kept at 30-35℃, sealed and fermented, and the fermentation time is 24-36h; Step 5: crush the fermented material and steam it, with the steaming temperature being ≥100°C and the steaming time being ≥10min; Step 6: Secondary fermentation: Treat the material according to step 5, add 1.5-3% of fermentation bacteria (Rhizopus), and mix well during the stirring process; Step 7: The inoculated material should be ≤15cm thick, heated at 30-35℃, with a fan speed of 70-100 rpm and a fermentation time of ≤30 hours; Step 8: Perform colloid mill refining treatment according to the material-liquid ratio of 1:5-6, and the material particle size is required to be 50-150 microns; Step 9: Spray drying: air inlet temperature 175-200°C, air outlet temperature 120-135°C, collect the material; Step 10: The collected spray-dried material is quantitatively packaged.

[0007] Specifically, in step 1, the temperature of water should be ≥95°C.

[0008] Specifically, the particle size of the material after crushing is ≤5mm.

[0009] The beneficial effects of the present invention are: Compared to single enzymatic peptide products, the postbiotic- and peptide-rich products produced through this fermentation-based postbiotic peptide preparation process exhibit a more powerful synergistic effect in regulating human physiological functions. Postbiotics regulate the intestinal microbiome, creating a favorable internal environment for peptides to better exert their immune-modulating and antioxidant effects. The two complement each other, promoting a more comprehensive health boost and providing consumers with a superior nutritional and health option. During the production process, this process uses cereal substrates, which are widely available and relatively inexpensive. This effectively controls production costs while ensuring product quality, making it suitable for large-scale industrial production. While the multi-step fermentation process may appear complex, the conditions of each step are clearly defined and easily controlled, with excellent stability and reproducibility, ensuring consistent quality across batches. DETAILED DESCRIPTION

[0010] The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

[0011] Unless otherwise specified, the technical solutions described in the present invention are all conventional solutions in the field; the reagents or materials described are all from commercial channels unless otherwise specified.

[0012] A process for preparing postbiotic polypeptides after fermentation, comprising the following steps: Step 1: Blanch and stir the cereal substrate in a ratio of 1:0.8-1.1 of material to water (temperature ≥95°C); Step 2: Steam the blanched and stirred material until cooked, with the cooking temperature being ≥ 100°C and the cooking time being ≥ 30 minutes; Step 3: Inoculation of matured materials: Add 2-4‰ of fermentation bacteria (Lactobacillus plantarum) to the treated materials according to step 2, stirring at 50-80 rpm until the mixture is evenly mixed. Step 4: Loading and fermentation: the material thickness is 20-25cm, the temperature is kept at 30-35℃, sealed and fermented, and the fermentation time is 24-36h; Step 5: crush the fermented material (particle size ≤ 5mm) and steam it at a temperature ≥ 100°C for a time ≥ 10 minutes; Step 6: Secondary fermentation: after processing the material according to step 5, add 1.5-3% of the fermentation bacteria (Rhizopus) and mix well during the stirring process; Step 7: The inoculated material should be ≤15cm thick, heated at 30-35℃, with a fan speed of 70-100 rpm and a fermentation time of ≤30 hours; Step 8: Perform colloid mill refining treatment according to the material-liquid ratio of 1:5-6, and the material particle size is required to be 50-150 microns; Step 9: Spray drying: air inlet temperature 175-200℃, air outlet temperature 120-135℃, collect the material; Step 10: quantitatively package the collected spray-dried material.

[0013] In summary, the product produced by the fermentation process of this application is rich in postbiotics (microbial metabolites) and peptides. However, enzymatic peptide products on the market do not contain postbiotics (enzymatic hydrolysis cannot produce microbial metabolites).

[0014] The technical features of the above-mentioned embodiments can be combined arbitrarily. In order to make the description concise, not all possible combinations of the technical features in the above-mentioned embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0015] The above-described embodiments merely illustrate several implementations of the present invention, and while their descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the patent. It should be noted that a person skilled in the art would be able to make numerous variations and improvements without departing from the spirit of the present invention, all of which fall within the scope of protection of the present invention. Therefore, the scope of protection of the patent for this invention shall be determined by the appended claims.

Claims

1. A process for preparing postbiotic polypeptides from fermentation, characterized in that: The following steps are involved: Step 1: Blanch and stir the cereal substrate at a material:water ratio of 1:0.8-1.1; Step 2: Steam the blanched and stirred material until cooked, with the cooking temperature being ≥100°C and the cooking time being ≥30 min; Step 3: Inoculation of matured materials: Add 2-4‰ of fermentation bacteria (Lactobacillus plantarum) to the treated materials according to step 2, stirring at 50-80 rpm until the mixture is evenly mixed. Step 4: Loading and fermentation: the material thickness is 20-25cm, the temperature is kept at 30-35℃, sealed and fermented, and the fermentation time is 24-36h; Step 5: crush the fermented material and steam it, with the steaming temperature being ≥100°C and the steaming time being ≥10min; Step 6: Secondary fermentation: Process the material according to step 5, add 1.5-3% of fermentation bacteria (Rhizopus), and stir to mix evenly; Step 7: The inoculated material should be ≤15cm thick, heated at 30-35℃, with a fan speed of 70-100 rpm and a fermentation time of ≤30 hours; Step 8: Perform colloid mill refining treatment according to the material-liquid ratio of 1:5-6, and the material particle size is required to be 50-150 microns; Step 9: Spray drying: air inlet temperature 175-200°C, air outlet temperature 120-135°C, collect the material; Step 10: The collected spray-dried material is quantitatively packaged.

2. The process for preparing a postbiotic polypeptide from fermentation as claimed in claim 1, wherein: In step 1, the water temperature should be ≥95℃.

3. The process for preparing a postbiotic polypeptide from fermentation as claimed in claim 1, wherein: In step 5, the particle size of the material after crushing is ≤5mm.